CN109444395B - Platform for in-situ research and induction of tissue regeneration mechanism in vivo and application method thereof - Google Patents
Platform for in-situ research and induction of tissue regeneration mechanism in vivo and application method thereof Download PDFInfo
- Publication number
- CN109444395B CN109444395B CN201811291591.7A CN201811291591A CN109444395B CN 109444395 B CN109444395 B CN 109444395B CN 201811291591 A CN201811291591 A CN 201811291591A CN 109444395 B CN109444395 B CN 109444395B
- Authority
- CN
- China
- Prior art keywords
- rat
- platform
- tissue regeneration
- tissue
- situ
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B3/00—Apparatus for testing the eyes; Instruments for examining the eyes
- A61B3/10—Objective types, i.e. instruments for examining the eyes independent of the patients' perceptions or reactions
- A61B3/1005—Objective types, i.e. instruments for examining the eyes independent of the patients' perceptions or reactions for measuring distances inside the eye, e.g. thickness of the cornea
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B3/00—Apparatus for testing the eyes; Instruments for examining the eyes
- A61B3/10—Objective types, i.e. instruments for examining the eyes independent of the patients' perceptions or reactions
- A61B3/107—Objective types, i.e. instruments for examining the eyes independent of the patients' perceptions or reactions for determining the shape or measuring the curvature of the cornea
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
Abstract
A platform for in-situ research on tissue regeneration inducing mechanism in living body is composed of a living rat cornea, a region on said rat cornea for removing corneal epithelial cells, a rat corneal stroma layer in said region, a rat corneal stroma layer graft and a rat corneal stroma layer graft, which are separated from the surface of said rat corneal stroma layer graft and connected together, and the tissue engineering material to be researched and arranged in the cavity between said rat corneal stroma layer graft and the separated surface of said rat corneal stroma layer graft, and a connecting material for connecting said rat corneal stroma layer graft and said rat corneal stroma bed graft. The platform can be used for observing through a living body microscope, observing tissue slices and analyzing molecular biology, and can better realize the observation and evaluation of the interaction effect between the tissue engineering material and an organism.
Description
Technical Field
The invention relates to the field of tissue engineering, in particular to a platform for in-situ research and induction of tissue regeneration mechanism in vivo and a using method thereof.
Background
The keratopathy is the second most reversible and blinding ophthalmopathy after cataract in China and even in the world, and corneal transplantation is the only reliable and effective clinical vision recovery means. At present, cornea donors in China are seriously scarce, and although the cornea transplantation operation is widely accepted all over the world, the specific regeneration mechanism is not clear, and the research on the development of tissue engineering cornea and the cornea regeneration and repair mechanism is a difficult problem which is urgently needed to be solved by the current society.
The rat is a good experimental animal selection due to the fact that the growth cycle is fast, feeding is easy, and the genetic background is clear. The rat cornea is suitable for being used as a platform for in-situ research on the mechanism of induced tissue regeneration in vivo due to the immune exemption characteristic and the appropriate thickness of the cornea. The cornea has the characteristic of being transparent, so that the cornea can be directly observed by a slit lamp or an ophthalmic surgery microscope to observe the angiogenesis and scarring conditions. The techniques of corneal ablation and corneal RNA extraction are also well established and can be used to analyze changes in morphological structure and gene expression during tissue regeneration.
Most of the existing tissue engineering materials are researched on skin, bones and other parts, and the cornea is rarely used as a platform for in-situ research on the mechanism of induced tissue regeneration. Because the skeleton belongs to the internal structure of the body, if the internal situation of the skeleton needs to be continuously observed, the skin of an experimental part needs to be cut open, and the injury to experimental animals is large. The skin does not have transparent properties, which makes it difficult to observe pathological conditions inside the skin tissue. The cornea belongs to the external structure of the body, has the characteristic of transparency, and is a better platform for in-situ research on the mechanism of induced tissue regeneration of a living body.
Disclosure of Invention
The invention mainly aims to overcome the defects of the prior art and provide a platform for in-situ research on tissue regeneration induction mechanism in vivo and a using method thereof.
In order to achieve the purpose, the invention adopts the following technical scheme:
a platform for inducing tissue regeneration mechanism in vivo in situ research comprises a rat cornea of a living body, wherein the rat cornea is provided with a region from which corneal epithelial cells are removed, a rat corneal stroma layer in the region is provided with a rat corneal stroma layer graft and a rat corneal stroma layer graft which are separated from the surface formed by slicing from a set incision opening inwards and are still connected together, a tissue engineering material to be researched is arranged in a cavity formed between the rat corneal stroma layer graft and the separated surface of the rat corneal stroma layer graft, and the rat corneal stroma layer graft are connected through a connecting material at the incision opening.
Further:
the connecting material is an ophthalmic surgical suture or biological glue.
The area from which the corneal epithelial cells are removed is a circular area.
The rat corneal stroma in the corneal epithelial cell-removed region has a notch at the incision extending downward perpendicular to the surface of the rat corneal stroma, the notch forming a passage for the incision instrument to enter the rat corneal stroma.
The depth of the notch is 25-35 μm.
The depth of the notch is 30 μm.
The area from which corneal epithelial cells were removed was located in the central region of the rat cornea.
The method for using the living body in-situ research induced tissue regeneration mechanism platform observes the new vessels and the turbid condition of the rat cornea of the living body in-situ research induced tissue regeneration mechanism platform through a slit lamp or an ophthalmic surgery microscope, and observes the thickness of the rat cornea through OCT (optical coherence tomography) so as to comprehensively evaluate the effect of the studied tissue engineering material on the induction of the regeneration of the rat cornea tissue.
The method for using the living body in-situ research induced tissue regeneration mechanism platform effect comprises the steps of carrying out HE (high intensity intrinsic) dyeing or immunofluorescence dyeing on a rat corneal section of the living body in-situ research induced tissue regeneration mechanism platform through a paraffin section or a frozen section, observing cell distribution and tissue structure of the rat corneal section, and comprehensively evaluating the effect of a researched tissue engineering material on inducing the rat corneal tissue regeneration.
The method for in-situ research on the effect of the platform on the mechanism of inducing tissue regeneration by using the living body comprises the steps of extracting rat corneal tissue RNA of the platform on the mechanism of inducing tissue regeneration in situ research by using the living body, carrying out qPCR analysis, and analyzing the gene expression condition of the rat corneal tissue so as to comprehensively evaluate the effect of the researched tissue engineering material on inducing the rat corneal tissue regeneration.
The invention has the following beneficial effects:
the invention provides a platform for in-situ research and induction of tissue regeneration mechanism in vivo and a use method thereof.
Compared with the prior art, the invention has the following advantages:
the platform has immune-exempting characteristics, so that the interaction between tissue engineering materials and organisms can be better researched, and the failure of transplantation experiments caused by immune rejection is avoided. Also, since the cornea is a transparent, external body structure, it can be easily observed. The cornea section and RNA extraction can analyze the cornea tissue regeneration and repair effect and the gene expression condition.
The platform realizes the in-situ research of the living body to induce the tissue regeneration mechanism environment to construct, can be used for researching the effect of different materials for tissue regeneration and repair and the influence of the different materials on the immune rejection of the organism.
Drawings
FIG. 1 is a sectional view of a platform for in-situ study of tissue regeneration induction mechanism in vivo according to an embodiment of the present invention.
FIG. 2 is a detailed illustration of a platform for in vivo in situ tissue regeneration induction mechanism according to an embodiment of the present invention.
FIG. 3 is a three-dimensional overall view of a platform for in-situ study of induced tissue regeneration mechanism in vivo according to an embodiment of the present invention.
Fig. 4 to 6 are process illustrations of a platform for in-situ study of induced tissue regeneration mechanism in vivo according to an embodiment of the present invention.
Detailed Description
The embodiments of the present invention will be described in detail below. It should be emphasized that the following description is merely exemplary in nature and is not intended to limit the scope of the invention or its application.
Referring to fig. 1 to 6, in one embodiment, a platform for in situ study of induced tissue regeneration mechanism in vivo comprises a rat cornea of a living body, wherein the rat cornea is provided with a region from which corneal epithelial cells 1 are removed, a rat corneal stroma layer in the region is provided with a rat corneal stroma layer graft 5 and a rat corneal stroma layer graft 4 which are separated from the surface formed by slicing from a set incision on the inner side and are still connected together, a tissue engineering material 6 to be studied is arranged in a chamber formed between the rat corneal stroma layer graft 5 and a separation surface 3 of the rat corneal stroma layer graft 4, and the rat corneal stroma layer graft 5 and the rat corneal stroma layer graft 4 are connected through a connecting material 7 at the incision.
In a preferred embodiment, the connecting material 7 is an ophthalmic surgical suture or a bio-gel.
In a preferred embodiment, the area from which the corneal epithelial cells 1 are removed is a circular area.
In a preferred embodiment, the rat corneal stroma in the area from which the corneal epithelial cells 1 are removed has a notch 2 at the incision extending downward perpendicular to the surface of the rat corneal stroma, and the notch 2 forms a passage for the incision instrument to enter the rat corneal stroma.
In a preferred embodiment, the depth of the indentations is 25-35 μm.
In a more preferred embodiment, the depth of the indentation is 30 μm.
In a preferred embodiment, the area from which corneal epithelial cells are removed is located in the central region of the rat cornea.
In another embodiment, a method for in situ study of tissue regeneration mechanism-inducing platform in vivo using any of the previous embodiments is used to observe the neovascularization and opacity of rat cornea of the in situ study tissue regeneration mechanism-inducing platform in vivo by slit lamp or ophthalmic surgery microscope, and observe the thickness of rat cornea by OCT to comprehensively evaluate the effect of the studied tissue engineering material on the induction of tissue regeneration of rat cornea.
In yet another embodiment, a method for in situ study of the effect of the platform for inducing tissue regeneration mechanism in vivo as described in any one of the above embodiments is used, wherein rat corneal sections of the platform for inducing tissue regeneration mechanism in vivo are subjected to HE staining or immunofluorescence staining through paraffin sections or frozen sections, and the cell distribution and tissue structure of the rat corneal sections are observed, so as to comprehensively evaluate the effect of the studied tissue engineering material on inducing the regeneration of rat corneal tissue.
In yet another embodiment, a method for in situ research of the effect of tissue regeneration mechanism platform induction by using a living body as described in any of the previous embodiments, comprises the steps of extracting rat corneal tissue RNA from the tissue regeneration mechanism platform induction by in situ research, performing qPCR analysis, and analyzing the gene expression of rat corneal tissue to comprehensively evaluate the effect of the studied tissue engineering material on the induction of rat corneal tissue regeneration.
The invention provides a platform for in-situ research and induction of tissue regeneration mechanism in vivo and a use method thereof.
Specific embodiments of the present invention are further described below with reference to the accompanying drawings.
Referring to fig. 1 to 6, in one embodiment, a platform for in situ study of induced tissue regeneration mechanism in vivo comprises rat cornea, at least one chamber on the rat cornea is constructed, the rat cornea has five structures, non-scraped corneal epithelial cells 1, a 30 μm deep gap 2, a separation surface 3, a rat corneal stroma layer graft 4, and a rat corneal stroma layer graft 5. The rat corneal stroma layer graft 4 and the rat corneal stroma layer graft 5 are used for bearing the tissue engineering material to be researched. The chamber is sealed by a 10-0 ophthalmic surgical suture or biological glue, then the eyelid is sutured by a 5-0 ophthalmic surgical suture, the rat is prevented from scratching the surgical eye, eye drops are dripped every day, eye ointment is smeared, and inflammatory reaction is avoided.
The 1.5ml 10% chloral hydrate solution can be used for intraperitoneal injection, a rat is used for anesthesia, tetracaine hydrochloride eye drops are used for ocular surface anesthesia, a 3.0 mm gem puncture knife is used for creating a gap 2 with the depth of 30 mu m on the cornea of the rat, a 2.8 mm gem tunnel knife is used for horizontally separating the corneal stroma layer of the rat, an Aikang 10-0 ophthalmic suture is used for suturing the stroma layer, a Lingqiao 5-0 ophthalmic suture is used for suturing the eyelid, and Diabium (tobramycin dexamethasone eye drops) and erythromycin eye ointment are used for postoperative care, so that a complete living body in-situ research induced tissue regeneration mechanism platform is constructed.
Fig. 4 is a schematic diagram showing a basic structure of a platform for in-situ research of a living body to induce tissue regeneration mechanism, wherein a rat cornea is subjected to conventional skin disinfection, an iodine type III disinfectant is wiped for 3 minutes, 75% alcohol disinfection is performed for 3 minutes, tetracaine hydrochloride eye drops are dripped for 1 minute, a trephine with a diameter of 2 mm is used for pressing a clear mark on the rat cornea, a No. 10 round head blade is used for scraping corneal epithelial cells in the area, a 3.0 mm gemstone puncturing knife is used for creating a 30-micrometer-deep notch 2 in the rat corneal stroma layer, a 2.8 mm gemstone tunnel knife is used for separating the rat corneal stroma layer from the 30-micrometer-deep position, and the rat corneal stroma layer implant 4 and the rat corneal stroma layer implant 5 are obtained. Fig. 5 is a schematic view showing tissue engineering material transplantation of a platform for in-situ study of a living body to induce tissue regeneration mechanism, in which a 1.5 mm trephine is used to drill the tissue engineering material, a forceps is used to uncover a rat corneal stroma layer implant 5, and an iris restorer is used to place the tissue engineering material with the diameter of 1.5 mm on a planting bed. Fig. 6 is a diagram showing the closing after transplantation of tissue engineering material of a platform for in situ research of tissue regeneration induction mechanism of a living body, a rat corneal stroma layer graft 4 and a rat corneal stroma layer graft 5 are sutured by using an Aierkang 10-0 ophthalmic suture, and air is exhausted as much as possible in the suturing process so as to prevent air bubbles from appearing in the rat corneal stroma layer. The eyelid suture was performed with a 5-0 eye suture of lingqiao to prevent rats from scratching the operated eye due to pain or itching, and the eyelid suture was opened 2 days later. After operation, dianbixin (tobramycin dexamethasone eye drops) is dripped 3 times a day until 2 times a day after one week, and erythromycin eye ointment or other anti-infective eye ointments are smeared 1 time in the morning and at the evening respectively until 1 time a day after one week.
The platform for in-situ study of the in-vivo induced tissue regeneration mechanism can observe the vascularization degree and the turbid condition of the cornea under a slit lamp or an ophthalmic surgery microscope according to arrangement, observe the thickness of the cornea of a rat through OCT (optical coherence tomography), and judge the edema condition.
The rat cornea can be taken down and fixed in 4% of a poly methanol solution, and the rat cornea section is subjected to HE staining or immunofluorescence staining through paraffin section or frozen section, so that the cell distribution and the tissue structure of the rat cornea section are observed.
The rat cornea is subjected to qPCR analysis through extraction of tissue RNA, gene expression conditions of rat cornea tissues are analyzed, and the effect of the researched materials on induction of rat cornea tissue regeneration is comprehensively evaluated.
The rat has the advantages of fast growth cycle, easy feeding, clear genetic background and the like, and the cornea of the rat has immune exemption characteristic and proper thickness of the cornea, and can well bear tissue engineering materials. The rat cornea is transparent, making in vitro observation easy. The application of molecular biology technology is helpful for the research on the effect of tissue engineering materials and the tissue regeneration mechanism.
The foregoing is a more detailed description of the invention in connection with specific/preferred embodiments and is not intended to limit the practice of the invention to those descriptions. It will be apparent to those skilled in the art that various substitutions and modifications can be made to the described embodiments without departing from the spirit of the invention, and these substitutions and modifications should be considered to fall within the scope of the invention.
Claims (10)
1. A platform for inducing tissue regeneration mechanism in vivo in situ research is characterized by comprising a rat cornea of a living body, wherein the rat cornea is provided with a region from which corneal epithelial cells are removed, a rat corneal stroma layer in the region is provided with a rat corneal stroma layer graft and a rat corneal stroma layer graft which are separated from the surface formed by slicing from a set incision inwards and are still connected together, a tissue engineering material to be researched is arranged in a cavity formed between the rat corneal stroma layer graft and the separated surface of the rat corneal stroma layer graft so as to induce the regeneration of rat corneal tissue through the tissue engineering material, and the rat corneal stroma layer graft are connected through a connecting material at the incision.
2. The platform for in vivo in situ tissue regeneration induction mechanism according to claim 1, wherein the connecting material is an ophthalmic surgical suture or a biological glue.
3. The platform for in vivo in situ tissue regeneration induction mechanism according to claim 1 or 2, wherein the area from which corneal epithelial cells are removed is a circular area.
4. The platform of any one of claims 1 to 2, wherein the rat corneal stroma in the corneal epithelial cell-removed region has a notch extending downward perpendicular to the surface of the rat corneal stroma at the incision, and the notch forms a passage for the incision instrument to enter the rat corneal stroma.
5. The platform of claim 4, wherein the depth of the gap is 25-35 μm.
6. The platform for in vivo in situ study of induced tissue regeneration mechanism as claimed in claim 5, wherein the depth of said gap is 30 μm.
7. The in vivo in situ study induced tissue regeneration mechanism platform of any one of claims 1-2, wherein said area from which corneal epithelial cells are removed is located in the central region of the rat cornea.
8. A method of using the platform for in situ study of induced tissue regeneration mechanisms in vivo according to any of claims 1-7, comprising: the new blood vessels and the turbid condition of the rat cornea of the living body in-situ research induction tissue regeneration mechanism platform are observed through a slit lamp or an ophthalmic surgery microscope, and the thickness of the rat cornea is observed through OCT (optical coherence tomography) so as to comprehensively evaluate the effect of the researched tissue engineering material on the induction of the rat cornea tissue regeneration.
9. A method of using the living body of any one of claims 1-7 for in situ studies of the effects of the platform of the mechanism inducing tissue regeneration, comprising: and carrying out HE (high intensity intrinsic phase) dyeing or immunofluorescence dyeing on the rat corneal section of the living body in-situ research induction tissue regeneration mechanism platform through a paraffin section or a frozen section, and observing cell distribution and tissue structure of the rat corneal section to comprehensively evaluate the effect of the researched tissue engineering material on induction of rat corneal tissue regeneration.
10. A method of using the living body of any one of claims 1-7 for in situ studies of the effects of the platform of the mechanism inducing tissue regeneration, comprising: the rat corneal tissue RNA of the tissue regeneration mechanism platform is induced by in-situ research of the living body, qPCR analysis is carried out, and the gene expression condition of the rat corneal tissue is analyzed so as to comprehensively evaluate the effect of the researched tissue engineering material on the induction of the rat corneal tissue regeneration.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811291591.7A CN109444395B (en) | 2018-10-31 | 2018-10-31 | Platform for in-situ research and induction of tissue regeneration mechanism in vivo and application method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811291591.7A CN109444395B (en) | 2018-10-31 | 2018-10-31 | Platform for in-situ research and induction of tissue regeneration mechanism in vivo and application method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109444395A CN109444395A (en) | 2019-03-08 |
| CN109444395B true CN109444395B (en) | 2021-11-16 |
Family
ID=65549252
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811291591.7A Active CN109444395B (en) | 2018-10-31 | 2018-10-31 | Platform for in-situ research and induction of tissue regeneration mechanism in vivo and application method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109444395B (en) |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100333702C (en) * | 2004-04-28 | 2007-08-29 | 浙江大学医学院附属邵逸夫医院 | Exogenous cornea substrate without cells and its preparation method and use |
| CN101433478B (en) * | 2007-11-14 | 2012-05-23 | 上海交通大学医学院附属第九人民医院 | Whole layer biological cornea as well as construction method and use thereof |
| US20100080840A1 (en) * | 2008-07-31 | 2010-04-01 | Michael Cho | Hybrid superporous hydrogel scaffold for cornea regeneration |
| CN102294053B (en) * | 2011-07-24 | 2014-10-29 | 陕西省眼科研究所 | Acellular heterogeneous corneal stroma carrier and preparation method and application thereof |
| CN107929811A (en) * | 2017-12-15 | 2018-04-20 | 厦门大学 | A kind of tissue engineering comea |
-
2018
- 2018-10-31 CN CN201811291591.7A patent/CN109444395B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN109444395A (en) | 2019-03-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP7336885B2 (en) | Biocompatible substrates and methods of implanting same that promote interconnection between stem cells and target tissues | |
| Edwards et al. | A thin slice preparation for patch clamp recordings from neurones of the mammalian central nervous system | |
| US11110008B2 (en) | Intraocular device for dual incisions | |
| US4919664A (en) | Stimulation of hair growth | |
| Avadhanam et al. | A brief review of Boston type-1 and osteo-odonto keratoprostheses | |
| CN107582139A (en) | The method and apparatus of tissue transplantation | |
| CN108697436A (en) | Ophthalmology knife and application method | |
| CN103272274B (en) | Biological repair tablet for herniae and preparation method thereof | |
| KR20130098888A (en) | Method and apparatus for tissue grafting and copying | |
| CN106725679B (en) | A kind of kiss/closure mouth reinforcement and repair sub-assembly and its preparation and application | |
| CN104288837B (en) | A kind of acellular dermal matrix and its production and use | |
| AU653499B2 (en) | Surgical instrument and cell isolation and transplantation | |
| CN109444395B (en) | Platform for in-situ research and induction of tissue regeneration mechanism in vivo and application method thereof | |
| CN102697581B (en) | Method for constructing tissue engineering blood vessel | |
| RU2734280C1 (en) | Method for isolated extraction and fabrication of histological structures of vitreous body | |
| CN103272275B (en) | Biological repair tablet for endocranium and preparation method thereof | |
| CN103301507B (en) | Artificial biological tendon and preparation method thereof | |
| CN111035807A (en) | Preparation method of ultrathin APCS (amorphous silicon copper-zinc copper) implant | |
| CN102424817B (en) | Cell strain from human lung adenocarcinoma and preparation method thereof | |
| CN114088493A (en) | Animal eyeball pathological section manufacturing method | |
| CN103234774B (en) | Method for in vitro separation and preparation of retina nerve cell layer | |
| Catanese et al. | Fluorescent in situ hybridization (FISH) on corneal impression cytology specimens (CICS): Study of epithelial cell survival after keratoplasty | |
| RU2398555C1 (en) | Method of anterior deep layer-by-layer keratoplasty | |
| RU2807919C1 (en) | Method of obtaining biopsy material for intraocular formations | |
| RU2459601C1 (en) | Method of performing complete stromectomy of cornea |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |