CN109438530A - A kind of preparation method of phosphorylation maltose - Google Patents
A kind of preparation method of phosphorylation maltose Download PDFInfo
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- CN109438530A CN109438530A CN201811461205.4A CN201811461205A CN109438530A CN 109438530 A CN109438530 A CN 109438530A CN 201811461205 A CN201811461205 A CN 201811461205A CN 109438530 A CN109438530 A CN 109438530A
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- maltose
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H3/00—Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
- C07H3/04—Disaccharides
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B4/00—General methods for preserving meat, sausages, fish or fish products
- A23B4/14—Preserving with chemicals not covered by groups A23B4/02 or A23B4/12
- A23B4/18—Preserving with chemicals not covered by groups A23B4/02 or A23B4/12 in the form of liquids or solids
- A23B4/20—Organic compounds; Microorganisms; Enzymes
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/02—Phosphorylation
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- Engineering & Computer Science (AREA)
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- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
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- General Chemical & Material Sciences (AREA)
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- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Meat, Egg Or Seafood Products (AREA)
Abstract
A kind of preparation method of phosphorylation maltose, it is characterized in that the preparation method includes the following steps: the preparation of 1) phosphorylation agent: by sodium tripolyphosphate and sodium trimetaphosphate mixture according to mass ratio be 10-15:1 after mixing, then being made into mass concentration is 15-20%(w/v) aqueous solution reagent；2) maltose is added in the phosphorylation agent of the above preparation number, makes its mixed 10-15%(w/v of mass concentration), after completely dissolution, adjusting mixed system pH is 7.5-8.5；The features such as it has method simply, reliably, energy improvement effect, and relevant metal ions and phosphate radical usage amount can be greatly reduced.
Description
Technical field:
This hair is related to a kind of preparation method of phosphorylation maltose, belongs to organic matter manufacturing technology field.
Background technique
In China's freezing shrimp meat production process, phosphoric acid salt is widely used as water-retaining agent and antifreeze, but phosphatic
On the one hand being excessively used will lead to deterioration in quality, on the other hand the intake of excessive P elements also has certain damage to human body.
It is phosphatic using brought negative effect, the both at home and abroad research and development of numerous and confused expansion phosphate-free water-retaining agent to reduce.Wheat
Bud sugar can be used as a kind of carbohydrate cryoprotective agent, ensure frozen fish quality, but its function and effect is relatively limited, substantially less than
Phosphoric acid salt；Meanwhile maltose has certain sweet taste, excessive addition will affect product taste.
Summary of the invention:
It is an object of the invention to overcome the shortcomings of the prior art, and it is simple, reliable to provide a kind of preparation method, can improve
The preparation method and application of the phosphorylation maltose of relevant metal ions and phosphate radical usage amount can be greatly reduced in effect.This hair
Bright purpose is completed by following technical solution, a kind of preparation method of phosphorylation maltose, the preparation method
Include the following steps:
1) preparation of phosphorylation agent: sodium tripolyphosphate and sodium trimetaphosphate mixture are mixed according to mass ratio for 10-15:1
After uniformly, then being made into mass concentration is 15-20%(w/v) aqueous solution reagent；
2) maltose is added in the phosphorylation agent of the above preparation number, makes its mixed 10-15%(w/ of mass concentration
V), after completely dissolution, adjusting mixed system pH is 7.5-8.5；
3) mixed system is placed in thermostatic control oscillator vibration, after 90-120 DEG C of water bath with thermostatic control concussion (revolving speed) reacts 1-5 h,
It takes out；
4) into mixed system, 95% ethyl alcohol of 2-4 times of volumes of addition, 20-28h of alcohol precipitation in 4 DEG C of refrigerators；4500-after alcohol precipitation
5500r/min is centrifuged 15min, is dissolved in a small amount of distilled water after taking precipitating, until can be completely dissolved；It is then transferred to bag filter
In, it dialyses, replaces distilled water at regular intervals, to remove unreacted phosphate ion in mixed system；
5) it dialyses after 24-72h, mixed liquor is freeze-dried, obtain phosphorylation maltose sample.
As preferred: in the step 1): according to mass ratio it is 11 by sodium tripolyphosphate and sodium trimetaphosphate mixture:
1 after mixing, then is made into mass concentration as 18%(w/v), 500mL aqueous solution reagent；
In the step 2), mixed mass concentration is 18%(w/v), adjusting mixed system pH is 8.0；The step
3) it in, after 70 DEG C of water bath with thermostatic control concussion (revolving speed) reaction 3h, takes out；
In the step 4), 95% ethyl alcohol of 2 times of volumes is added, and alcohol precipitation is for 24 hours in 4 DEG C of refrigerators；Alcohol precipitation 5000r/min centrifugation
15min；
It in the step 5), dialyses after 48h, mixed liquor is freeze-dried, obtain phosphorylation maltose sample.
Phosphorylation maltose of the present invention is applied in the preprocessing process of frozen fish, passes through maltose+phosphorus
The synergistic protective effect of acid ion improves low-temperature protection effect；Meanwhile compared to phosphate is used merely, can be greatly reduced
The usage amount of relevant metal ions and phosphate radical.
Phosphorylation maltose application of the present invention is: phosphorylation maltose being configured to 7-10% aqueous solution, adjusts pH
Value is 8.5-9.5；Freezing shrimp meat is immersed in phosphorylation maltose solution, solution is that 1:9-10 speed is stirred with peeled shrimp volume ratio
Under the conditions of mixing, in -20 DEG C or less cold storage after immersion 15-20 progress is quick-frozen at a temperature of 4 DEG C.
As preferred: the phosphorylation maltose application is: phosphorylation maltose being configured to 8% aqueous solution, adjusts pH
Value is 9.0；Freezing shrimp meat is immersed in phosphorylation maltose solution, solution and peeled shrimp volume ratio are 1:9, mix slowly condition
Under, after impregnating 18h at a temperature of 4 DEG C, in -25 DEG C of cold storage after taking-up progress is quick-frozen.
Specific embodiment
Below with reference to specific implementation example, the present invention is described in detail；A kind of preparation method of phosphorylation maltose,
It is characterized in that the preparation method includes the following steps:
1) preparation of phosphorylation agent: sodium tripolyphosphate and sodium trimetaphosphate mixture are mixed according to mass ratio for 10-15:1
After uniformly, then being made into mass concentration is 15-20%(w/v) aqueous solution reagent；
2) maltose is added in the phosphorylation agent of the above preparation number, makes its mixed 10-15%(w/ of mass concentration
V), after completely dissolution, adjusting mixed system pH is 7.5-8.5；
3) mixed system is placed in thermostatic control oscillator vibration, after 90-120 DEG C of water bath with thermostatic control concussion (revolving speed) reacts 1-5 h,
It takes out；
4) into mixed system, 95% ethyl alcohol of 2-4 times of volumes of addition, 20-28h of alcohol precipitation in 4 DEG C of refrigerators；4500-after alcohol precipitation
5500r/min is centrifuged 15min, is dissolved in a small amount of distilled water after taking precipitating, until can be completely dissolved；It is then transferred to bag filter
In, it dialyses, replaces distilled water at regular intervals, to remove unreacted phosphate ion in mixed system；
5) it dialyses after 24-72h, mixed liquor is freeze-dried, obtain phosphorylation maltose sample.
Preferred embodiments: in the step 1): being according to mass ratio by sodium tripolyphosphate and sodium trimetaphosphate mixture
11:1 after mixing, then is made into mass concentration as 18%(w/v), 500mL aqueous solution reagent；
In the step 2), mixed mass concentration is 18%(w/v), adjusting mixed system pH is 8.0；The step
3) it in, after 70 DEG C of water bath with thermostatic control concussion (revolving speed) reaction 3h, takes out；
In the step 4), 95% ethyl alcohol of 2 times of volumes is added, and alcohol precipitation is for 24 hours in 4 DEG C of refrigerators；Alcohol precipitation 5000r/min centrifugation
15min；
It in the step 5), dialyses after 48h, mixed liquor is freeze-dried, obtain phosphorylation maltose sample.
Phosphorylation maltose of the present invention is applied in the preprocessing process of frozen fish, passes through maltose+phosphorus
The synergistic protective effect of acid ion improves low-temperature protection effect；Meanwhile compared to phosphate is used merely, can be greatly reduced
The usage amount of relevant metal ions and phosphate radical.
Phosphorylation maltose application of the present invention is: phosphorylation maltose being configured to 7-10% aqueous solution, adjusts pH
Value is 8.5-9.5；Freezing shrimp meat is immersed in phosphorylation maltose solution, solution is that 1:9-10 speed is stirred with peeled shrimp volume ratio
Under the conditions of mixing, in -20 DEG C or less cold storage after immersion 15-20 progress is quick-frozen at a temperature of 4 DEG C.
The preferably described phosphorylation maltose application of the present invention is: phosphorylation maltose being configured to 8% aqueous solution, is adjusted
PH value is 9.0；Freezing shrimp meat is immersed in phosphorylation maltose solution, solution and peeled shrimp volume ratio are 1:9, mix slowly item
Under part, after impregnating 18h at a temperature of 4 DEG C, in -25 DEG C of cold storage after taking-up progress is quick-frozen.
Claims (5)
1. a kind of preparation method of phosphorylation maltose, it is characterised in that the preparation method includes the following steps:
1) preparation of phosphorylation agent: sodium tripolyphosphate and sodium trimetaphosphate mixture are mixed according to mass ratio for 10-15:1
After uniformly, then being made into mass concentration is 15-20%(w/v) aqueous solution reagent；
2) maltose is added in the phosphorylation agent of the above preparation number, makes its mixed 10-15%(w/ of mass concentration
V), after completely dissolution, adjusting mixed system pH is 7.5-8.5；
3) mixed system is placed in thermostatic control oscillator vibration, after 90-120 DEG C of water bath with thermostatic control concussion (revolving speed) reacts 1-5 h,
It takes out；
4) into mixed system, 95% ethyl alcohol of 2-4 times of volumes of addition, 20-28h of alcohol precipitation in 4 DEG C of refrigerators；4500-after alcohol precipitation
5500r/min is centrifuged 15min, is dissolved in a small amount of distilled water after taking precipitating, until can be completely dissolved；It is then transferred to bag filter
In, it dialyses, replaces distilled water at regular intervals, to remove unreacted phosphate ion in mixed system；
5) it dialyses after 24-72h, mixed liquor is freeze-dried, obtain phosphorylation maltose sample.
2. the preparation method of phosphorylation maltose according to claim 1, it is characterised in that: in the step 1): will
Sodium tripolyphosphate and sodium trimetaphosphate mixture according to mass ratio be 11:1 after mixing, then being made into mass concentration is 18%(w/
V), 500mL aqueous solution reagent；
In the step 2), mixed mass concentration is 18%(w/v), adjusting mixed system pH is 8.0；The step
3) it in, after 70 DEG C of water bath with thermostatic control concussion (revolving speed) reaction 3h, takes out；
In the step 4), 95% ethyl alcohol of 2 times of volumes is added, and alcohol precipitation is for 24 hours in 4 DEG C of refrigerators；Alcohol precipitation 5000r/min centrifugation
15min；
It in the step 5), dialyses after 48h, mixed liquor is freeze-dried, obtain phosphorylation maltose sample.
3. a kind of application of phosphorylation maltose as claimed in claim 1 or 2, it is characterised in that the phosphorylation maltose is answered
For by maltose+phosphate anion synergistic protective effect, improving low-temperature protection in the preprocessing process of frozen fish
Effect；Meanwhile compared to phosphate is used merely, the usage amount of relevant metal ions and phosphate radical can be greatly reduced.
4. the application of phosphorylation maltose according to claim 3, it is characterised in that the phosphorylation maltose application
It is: phosphorylation maltose is configured to 7-10% aqueous solution, adjusting pH value is 8.5-9.5；Freezing shrimp meat is immersed in phosphorylation
In maltose solution, solution and peeled shrimp volume ratio are that it is quick-frozen that 15-20 progress is impregnated at a temperature of 4 DEG C under 1:9-10 speed stirring condition
Afterwards in -20 DEG C or less cold storage.
5. the application of phosphorylation maltose according to claim 4, it is characterised in that the phosphorylation maltose application
It is: phosphorylation maltose is configured to 8% aqueous solution, adjusting pH value is 9.0；It is molten that freezing shrimp meat is immersed in phosphorylation maltose
In liquid, solution and peeled shrimp volume ratio are 1:9, under the conditions of mixing slowly, after impregnating 18h at a temperature of 4 DEG C, after taking-up progress is quick-frozen
In -25 DEG C of cold storage.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811461205.4A CN109438530A (en) | 2018-12-01 | 2018-12-01 | A kind of preparation method of phosphorylation maltose |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811461205.4A CN109438530A (en) | 2018-12-01 | 2018-12-01 | A kind of preparation method of phosphorylation maltose |
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| CN109438530A true CN109438530A (en) | 2019-03-08 |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105601760A (en) * | 2016-03-14 | 2016-05-25 | 南京农业大学 | Preparation method of phosphorylated epimedium polysaccharide |
| CN108003254A (en) * | 2017-12-14 | 2018-05-08 | 浙江海洋大学 | A kind of preparation method and application of phosphorylation carrageenan oligosaccharide |
-
2018
- 2018-12-01 CN CN201811461205.4A patent/CN109438530A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105601760A (en) * | 2016-03-14 | 2016-05-25 | 南京农业大学 | Preparation method of phosphorylated epimedium polysaccharide |
| CN108003254A (en) * | 2017-12-14 | 2018-05-08 | 浙江海洋大学 | A kind of preparation method and application of phosphorylation carrageenan oligosaccharide |
Non-Patent Citations (1)
| Title |
|---|
| ALEXANDER APELBLAT: "A new two-parameter equation for correlation and prediction of densities as a function of concentration and temperature in binary aqueous solutions", 《JOURNAL OF MOLECULAR LIQUIDS》 * |
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