CN109432484A - Medical super-hydrophobic antiseptic dressing of one kind and preparation method thereof - Google Patents
Medical super-hydrophobic antiseptic dressing of one kind and preparation method thereof Download PDFInfo
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- CN109432484A CN109432484A CN201811229292.0A CN201811229292A CN109432484A CN 109432484 A CN109432484 A CN 109432484A CN 201811229292 A CN201811229292 A CN 201811229292A CN 109432484 A CN109432484 A CN 109432484A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/46—Deodorants or malodour counteractants, e.g. to inhibit the formation of ammonia or bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/18—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing inorganic materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/20—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing organic materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/26—Macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/10—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing inorganic materials
- A61L2300/102—Metals or metal compounds, e.g. salts such as bicarbonates, carbonates, oxides, zeolites, silicates
- A61L2300/104—Silver, e.g. silver sulfadiazine
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/04—Materials for stopping bleeding
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/12—Nanosized materials, e.g. nanofibres, nanoparticles, nanowires, nanotubes; Nanostructured surfaces
Abstract
The present invention relates to a kind of medical super-hydrophobic antiseptic dressings and preparation method thereof, are related to medical product field.The preparation method of medical super-hydrophobic antiseptic dressing includes: dressing to be placed in the alkaline buffer containing dopamine the first dressing sample by poly-dopamine modified lithium is made using the autohemagglutination of dopamine.First dressing sample is placed in the solution of fluorine-bearing modifier react the poly-dopamine modified lithium through fluorine modification the second dressing sample.Second dressing sample, which is placed in reaction in silver ion solution, makes silver ion be reduced to Nano silver grain in poly-dopamine, easy to operate controllable, and controlling dressing by this method can sterilize in the different phase of wound healing to reach, the purpose of anti-adhesive.Medical super-hydrophobic antiseptic dressing provided by the invention can be used as acute wound or infective wound surface dressing with preferable hydrophobicity and antibiotic property.
Description
Technical field
The present invention relates to medical product fields, and in particular to a kind of medical super-hydrophobic antiseptic dressing and preparation method thereof.
Background technique
Gauze is one of common used material of hospital, and what present hospital generally used is all single layer absorbent gauze, when in use
Time has the following problems: firstly, woven structure method traditionally, retractility is poor, and gauze is made by cotton fiber, such as
Fruit is changed without for a long time, and bacterium easy to breed causes wound subinfection again, slows down the healing rate of wound;Secondly as adopting
It with single layer degreasing cotton structure, easily absorbs water when encountering water, if protecting accidentally in field when binding up a wound, is easy to cause wound
Inflammation, while the secretion of wound and gauze are adhered together, are not easy to remove gauze from wound, when removing gauze to trouble
Person causes very big pain, and is not easy ventilative, easily breeds germ in the environment of hot humid, wound is caused to feel again
Dye, very big injury is caused to patient, in Current prevention and cure technology, antibacterial generally reaches target effect using silver ion, simultaneously
Can promote blood circulation, eliminate or the sense that significantly lessens fatigue, be conducive to the quick healing of wound, but metal ion generally for
Biology is more toxic.In view of the above problems, the present invention is made that some improvement to existing gauze.
Summary of the invention
The purpose of the present invention is to provide a kind of medical super-hydrophobic antiseptic dressings, with preferable hydrophobicity and antibacterial
Property, it can be used as acute wound or infective wound surface dressing.
Another object of the present invention is to provide a kind of preparation method of medical super-hydrophobic antiseptic dressing, it is easy to operate can
Control, controlling dressing by this method can sterilize in the different phase of wound healing to reach, the purpose of anti-adhesive.
The present invention solves its technical problem and adopts the following technical solutions to realize.
The present invention proposes a kind of preparation method of medical super-hydrophobic antiseptic dressing comprising:
Dressing is placed in the alkaline buffer containing dopamine and is made using the autohemagglutination of dopamine by poly-dopamine modified lithium
The first dressing sample.
First dressing sample is placed in the solution of fluorine-bearing modifier react the poly-dopamine modified lithium through fluorine modification
Two dressing samples.
Second dressing sample, which is placed in reaction in silver ion solution, makes silver ion be reduced to silver nanoparticle in poly-dopamine
Particle.
The present invention proposes a kind of medical super-hydrophobic antiseptic dressing, by the preparation method of above-mentioned medical super-hydrophobic antiseptic dressing
It is made.
The present invention proposes a kind of medical super-hydrophobic antiseptic dressing comprising dressing and antibacterial hydrophobic unit, antibacterial hydrophobic list
For member by constituting through fluorine modification and doped with the poly-dopamine of Nano silver grain, antibacterial hydrophobic unit is attached to dressing through poly-dopamine
Fiber surface.
The beneficial effect of the medical super-hydrophobic antiseptic dressing of the embodiment of the present invention and preparation method thereof is:
Operation of the present invention is simple, and material is easy to get, and utilization rate is high, by changing dopamine and silver ion solution concentration, that is, adjustable
Save load capacity;Nano silver grain made of the present invention is uniform and is firmly covered in gauze, therefore can save use for a long time;
It can be applied to surface of a wound promotion healing by the way that the gauze dressing of Nano silver grain will be loaded;And the concentration of silver ion solution is in 16mg/
Equal no cytotoxicity, safer within ml, while super-hydrophobic gauze made of the present invention does not adhere to blood, therefore is not present two
Secondary damage.Hydrophobicity fluorine component on the chain of poly-dopamine can promote polymer chain hydrophobic patch and deeper penetrate into membrane lipid
In domain, lead to film decomposition and cell death, to prevent bacteria planting and enhance antibacterial ability;To sum up, after reaching trauma surface infestation,
Dressing can achieve the effect of bactericidal haemostatic;It heals the later period, dressing does not adhere to wound, can tear easily.
Detailed description of the invention
In order to illustrate the technical solution of the embodiments of the present invention more clearly, below will be to needed in the embodiment attached
Figure is briefly described, it should be understood that the following drawings illustrates only certain embodiments of the present invention, therefore is not construed as pair
The restriction of range for those of ordinary skill in the art without creative efforts, can also be according to this
A little attached drawings obtain other relevant attached drawings.
Fig. 1 is the hydrophobicity comparative effectiveness figure for the different gauze dressings that test example 1 of the present invention provides;
Fig. 2 is antibacterial effect figure of the different gauze dressings that provide of test example 2 of the present invention to staphylococcus aureus;
Fig. 3 is the different gauze dressing surface of a wound bacterium number comparison schematic diagrams that test example 3 of the present invention provides;
Fig. 4 is antibacterial effect figure of the different gauze dressings that provide of test example 4 of the present invention to Escherichia coli;
Fig. 5 is the change dopamine that test example 5 of the present invention provides, AgNO3Concentration is dredged with adjustment fluorine-bearing modifier ratio
Aqueous comparison schematic diagram.
Specific embodiment
It in order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below will be in the embodiment of the present invention
Technical solution be clearly and completely described.The person that is not specified actual conditions in embodiment, according to normal conditions or manufacturer builds
The condition of view carries out.Reagents or instruments used without specified manufacturer is the conventional production that can be obtained by commercially available purchase
Product.
Medical super-hydrophobic antiseptic dressing of the embodiment of the present invention and preparation method thereof is specifically described below.
The present invention provides a kind of preparation method of medical super-hydrophobic antiseptic dressing comprising:
S1. dressing is placed in alkalescent buffer with dopamine or DOPA amine salt and is made using the autohemagglutination of dopamine by gathering
The first modified dressing sample of dopamine.It that is to say, by the oxidative polymerization of dopamine under alkaline condition, in dressing
Stop surface uniformly grows a strata dopamine film.The poly-dopamine film has preferable biocompatibility.
Wherein, dressing by fiber it is woven or interweave and obtain, such as dressing be gauze product, cotton products, cloth products or
Other can be used for product made from the material of medical aspect, and the setting of different size can be carried out according to practical situation.The present invention
In preferred embodiment, dressing is selected from hospital gauze or medical non-woven fabrics, such as dressing is common medical absorbent cotton/gauze or dressing
For double mesostructure gauze dressings.
Dressing and dopamine or DOPA amine salt are placed in the step in alkaline buffer, it can be for first by dopamine or DOPA
Amine salt is dissolved in alkaline buffer, and then dressing is placed in the solution again, in order to guarantee that the autohemagglutination of dopamine is made by poly- DOPA
Amine can effectively be wrapped in the fiber surface of dressing, and in preferred embodiments of the present invention, dressing and dopamine or DOPA amine salt are set
Step in alkaline buffer includes:
After dressing is placed in alkaline buffer, dopamine or DOPA amine salt are added.Wherein, DOPA amine salt is preferably more
Bar amine hydrochlorate.
In preferred embodiments of the present invention, alkalescent buffer is the Tris buffer that pH value is 8-9;Such as pH value is
8.1,8.3,8.5,8.7 or 8.9 Tris buffer.The concentration of Tris buffer is, for example, 8-12mM, for example, 9,10,11 or
12mM etc..
Preferably, the concentration of addition of the dopamine in Tris buffer is 3-4.5mg/ml, such as 3mg/ml, 3.5mg/
Ml, 3.7mg/ml, 3.8mg/ml, 3.9mg/ml, 4mg/ml, 4.1mg/ml, 4.2mg/ml, 4.4mg/ml or 4.5mg/ml etc.,
Preferably, dressing is placed in the alkaline buffer containing dopamine and reacts 10-15h, such as 11h, 12h, 13h, 14h or 15h
Deng after, the first dressing sample is obtained.Under above-mentioned condition, self-polymeric reaction is good.
S2. the first dressing sample is placed in the solution of fluorine-bearing modifier and reacts to obtain the poly-dopamine modified lithium through fluorine modification
Second dressing sample.By poly-dopamine through fluorine grafting and modifying, the hydrophobicity of the second dressing sample is effectively improved.
In preferred embodiments of the present invention, before the first dressing sample is placed in the solution of fluorine-bearing modifier, removal the
The extra dopamine on one dressing sample surface, the mode rinsed for example, by using deionized water are removed, and are prevented to it to poly- more
Bar amine later period has an impact through fluorine modification and silver ion reduction, leads to the antibacterial hydrophobic of last medical super-hydrophobic antiseptic dressing
It is ineffective.Meanwhile before the first dressing sample is placed in silver ion solution, the first dressing sample surface of removal that is to say
It further include that rinse is carried out to the first dressing sample after extra dopamine, the solvent that rinse uses is molten for the solution of fluorine-bearing modifier
Agent.
In preferred embodiments of the present invention, fluorine-bearing modifier 1H, 1H, 2H, 2H-perfluor decyl mercaptan, perfluor trihydroxy methyl
Propane, perfluor polyethylene glycol, the fluoro- 1,4- benzene dimethanol of 2,3,5,6- tetra-, perfluor-n-butyl alcohol, perfluor -1- octanol, 1,4- fourth two
At least one of mercaptan and 1,6- ethanthiol;Preferably, fluorine-bearing modifier 1H, 1H, 2H, 2H-perfluor decyl mercaptan.
In preferred embodiments of the present invention, the solution of fluorine-bearing modifier is 1H, 1H, 2H, and 2H-perfluor decyl mercaptan ethyl alcohol is molten
Liquid, wherein 1H, 1H, 2H, 2H-perfluor decyl mercaptan addition volume are the 0.025%- of the liquor capacity of fluorine-bearing modifier
0.4%, such as 1H, 1H, 2H, 2H-perfluor decyl mercaptan addition volume be the liquor capacity of fluorine-bearing modifier 0.025%,
0.025%, 0.028%, 0.029%, 0.030%, 0.033%, 0.035%, 0.037%, 0.039% or 0.4% etc., effectively
Fluorine is grafted into poly-dopamine, the first dressing sample is modified.
S3. the second dressing sample is placed in reaction in silver ion solution makes silver ion be reduced to Yin Na in poly-dopamine
Rice corpuscles, doped with Nano silver grain in the poly-dopamine for the medical super-hydrophobic antiseptic dressing that is to say, antibacterial effect is good
And it is safer.
In preferred embodiments of the present invention, silver ion solution is selected from silver nitrate solution, silver acetate solution and silver lactate solutions
In any one;Preferably, silver ion solution is silver nitrate solution, is easier to be made, and it contains a large amount of silver ions, oxidisability
It is relatively strong, it is more conducive to be reduced.
Preferably, the concentration of silver ion is 7-16mg/ml in silver ion solution in preferred embodiments of the present invention;Such as
7.2mg/ml, 7.5mg/ml, 8mg/ml, 8.5mg/ml, 9mg/ml, 9.6mg/ml, 10mg/ml, 11mg/ml, 13mg/ml or
15.5mg/ml etc..It that is to say, the i.e. adjustable load capacity of the concentration by changing dopamine and silver ion solution meets different
Demand expands the range of application.
In preferred embodiments of the present invention, the second dressing sample will be placed in silver ion solution and react 3-8h, such as
3.5h, 4h, 4.5h, 5h, 5.5h, 6h, 7h or 8h etc., make silver ion be reduced to Nano silver grain in poly-dopamine.
In preferred embodiments of the present invention, the second dressing sample, which is placed in reaction in silver ion solution, makes silver ion poly- more
After being reduced to Nano silver grain on bar amine, further include by obtained sample with washing repeatedly, be lyophilized later, such as use deionization
Water washing 2,3,5 or 7 are inferior, are subsequently placed in after freezing 12h-36h under freezing environment, and freeze-drying removes moisture.Wherein, the present invention compared with
In good embodiment, freezing environment is lower than -20 DEG C, and effect is more preferably.
The present invention also provides a kind of medical super-hydrophobic antiseptic dressings, by the preparation side of above-mentioned medical super-hydrophobic antiseptic dressing
Method is made, and with preferable hydrophobicity and antibiotic property, can be used as acute wound or infective wound surface dressing.
The present invention also provides a kind of medical super-hydrophobic antiseptic dressings comprising dressing and antibacterial hydrophobic unit, antibacterial hydrophobic
By constituting through fluorine modification and doped with the poly-dopamine of Nano silver grain, antibacterial hydrophobic unit is attached to deposited unit through poly-dopamine
The fiber surface of material.It can be used as acute wound or infective wound surface dressing with preferable hydrophobicity and antibiotic property.
Feature and performance of the invention are described in further detail with reference to embodiments.
Embodiment 1
A kind of medical super-hydrophobic antiseptic dressing comprising dressing and antibacterial hydrophobic unit, antibacterial hydrophobic unit through fluorine by repairing
It adorns and is constituted doped with the poly-dopamine of Nano silver grain, antibacterial hydrophobic unit is attached to the fiber table of dressing through poly-dopamine
Face.
Medical super-hydrophobic antiseptic dressing is made by following methods:
S1. the common medical absorbent cotton/gauze of 50mg is immersed in the Tris buffer for the pH=8.5 that 30ml concentration is 10mM,
Then 120mg dopamine hydrochloride is added, reaction overnight 12h makes dopamine self-assemble on medical absorbent cotton/gauze, obtain by
First dressing sample of poly-dopamine modified lithium.
S2. the first dressing sample is washed with deionized 3 times, removes extra dopamine, and with dehydrated alcohol rinse,
Then being immersed in volume ratio is ethyl alcohol: 12h overnight inside 1H, 1H, 2H, 2H- perfluor decyl mercaptan=1:500 solution, obtain through
Second dressing sample of the poly-dopamine modified lithium of fluorine modification.Wherein 1H, 1H, 2H, 2H- perfluor decyl mercaptan alcohol mixture are total
20 μ L of 10ml, 1H, 1H, 2H, 2H- perfluor decyl mercaptan.
S3. the second dressing sample is immersed in concentration 8mg/mlAgNO3In solution, 5h is reacted, makes silver ion in poly- DOPA
It is reduced to Nano silver grain on amine, the sample obtained after reaction is washed with deionized 3 times, is lyophilized later.
Embodiment 2
A kind of medical super-hydrophobic antiseptic dressing comprising dressing and antibacterial hydrophobic unit, antibacterial hydrophobic unit through fluorine by repairing
It adorns and is constituted doped with the poly-dopamine of Nano silver grain, antibacterial hydrophobic unit is attached to the fiber table of dressing through poly-dopamine
Face.
Medical super-hydrophobic antiseptic dressing is made by following methods:
S1. the common medical absorbent cotton/gauze of 50mg is immersed in the Tris buffer for the pH=8.5 that 30ml concentration is 10mM,
Then 100mg dopamine hydrochloride is added, reaction overnight 12h makes dopamine self-assemble on medical absorbent cotton/gauze, obtain by
First dressing sample of poly-dopamine modified lithium.
S2. the first dressing sample is washed with deionized 3 times, removes extra dopamine, and with dehydrated alcohol rinse,
Then being immersed in volume ratio is ethyl alcohol: 12h overnight inside 1H, 1H, 2H, 2H- perfluor decyl mercaptan=1:300 solution, obtain through
Second dressing sample of the poly-dopamine modified lithium of fluorine modification.Wherein 1H, 1H, 2H, 2H- perfluor decyl mercaptan alcohol mixture are total
30 μ L of 10ml, 1H, 1H, 2H, 2H- perfluor decyl mercaptan.
S3., second dressing sample is immersed in the AgNO of concentration 6mg/ml3In solution, 5h is reacted, makes silver ion poly- more
It is reduced to Nano silver grain on bar amine, the sample obtained after reaction is washed with deionized at least 2 times, is lyophilized later.
Embodiment 3
S1. the common medical non-woven fabrics of 50mg is immersed in the Tris buffer for the pH=8.5 that 25ml concentration is 9mM, so
100mg dopamine hydrochloride is added afterwards, reaction overnight 12h makes dopamine self-assemble on medical absorbent cotton/gauze, obtains being gathered
The first modified dressing sample of dopamine.
S2. the first dressing sample is washed with deionized 3 times, removes extra dopamine, and with dehydrated alcohol rinse,
Then being immersed in volume ratio is ethyl alcohol: 12h overnight inside 1H, 1H, 2H, 2H- perfluor decyl mercaptan=1:1000 solution, obtain through
Second dressing sample of the poly-dopamine modified lithium of fluorine modification.Wherein 1H, 1H, 2H, 2H- perfluor decyl mercaptan alcohol mixture are total
10 μ L of 10ml, 1H, 1H, 2H, 2H- perfluor decyl mercaptan.
S3., second dressing sample is immersed in the AgNO of concentration 8mg/ml3In solution, 5h is reacted, makes silver ion poly- more
It is reduced to Nano silver grain on bar amine, the sample obtained after reaction is washed with deionized at least 2 times, is lyophilized later.
Embodiment 4
S1. the common medical absorbent cotton/gauze of 50mg is immersed in the Tris buffer for the pH=8.5 that 30ml concentration is 10mM,
Then 110mg dopamine hydrochloride is added, reaction overnight 13h makes dopamine self-assemble on medical absorbent cotton/gauze, obtain by
First dressing sample of poly-dopamine modified lithium.
S2. the first dressing sample is washed with deionized 3 times, removes extra dopamine, and with dehydrated alcohol rinse,
Then being immersed in volume ratio is ethyl alcohol: 12h overnight inside 1H, 1H, 2H, 2H- perfluor decyl mercaptan=1:800 solution, obtain through
Second dressing sample of the poly-dopamine modified lithium of fluorine modification.Wherein 1H, 1H, 2H, 2H- perfluor decyl mercaptan alcohol mixture are total
20 μ L of 10ml, 1H, 1H, 2H, 2H- perfluor decyl mercaptan.
S3. the second dressing sample is immersed in the AgNO3 solution of concentration 6.5mg/ml, reacts 5h, make silver ion poly-
It is reduced to Nano silver grain on dopamine, the sample obtained after reaction is washed with deionized at least 2 times, is lyophilized later.
Embodiment 5
The medical super-hydrophobic antiseptic dressing and the difference of embodiment 1 are only that:
The first dressing sample is washed with deionized 3 times in step S2., removes extra dopamine, and with anhydrous second
Alcohol rinse, being then immersed in volume ratio is ethyl alcohol: 12h is stayed overnight inside 1H, 1H, 2H, 2H- perfluor decyl mercaptan=1:250 solution,
Obtain the second dressing sample of the poly-dopamine modified lithium through fluorine modification.Wherein 1H, 1H, 2H, 2H- perfluor decyl mercaptan alcohol mixture
Total 10ml, 1H, 1H, 2H, 40 μ L of 2H- perfluor decyl mercaptan.
Embodiment 6
The medical super-hydrophobic antiseptic dressing and the difference of embodiment 5 are only that:
Second dressing sample is immersed in the AgNO of concentration 12mg/ml by step S3.3In solution, 5h is reacted, silver ion is made to exist
It is reduced to Nano silver grain in poly-dopamine, the sample obtained after reaction is washed with deionized at least 2 times, is frozen later
It is dry.
Embodiment 7
The medical super-hydrophobic antiseptic dressing and the difference of embodiment 1 are only that:
The first dressing sample is washed with deionized 4 times in step S2., removes extra dopamine, and with anhydrous second
Alcohol rinse, being then immersed in volume ratio is ethyl alcohol: staying overnight 12h inside perfluor -1- octanol=1:300 solution, obtains repairing through fluorine
Second dressing sample of the poly-dopamine modified lithium of decorations.Wherein perfluor -1- octanol alcohol mixture amounts to 20ml, perfluor -1- octanol
40μL。
Embodiment 8
The medical super-hydrophobic antiseptic dressing and the difference of embodiment 7 are only that:
The first dressing sample is washed with deionized 3 times in step S2, removes extra dopamine, and with anhydrous second
Alcohol rinse, being then immersed in volume ratio is ethyl alcohol: inside 1H, 1H, 2H, 2H- perfluor decyl mercaptan=1:1000 solution overnight
12h obtains the second dressing sample of the poly-dopamine modified lithium through fluorine modification.Wherein 1H, 1H, 2H, 2H- perfluor decyl mercaptan ethyl alcohol are mixed
It closes object and amounts to 10ml, 1H, 1H, 2H, 10 μ L of 2H- perfluor decyl mercaptan.
Embodiment 9
The medical super-hydrophobic antiseptic dressing and the difference of embodiment 8 are only that:
Second dressing sample is immersed in the AgNO of concentration 8mg/ml by step S3.3In solution, 5h is reacted, silver ion is made to exist
It is reduced to Nano silver grain in poly-dopamine, the sample obtained after reaction is washed with deionized at least 2 times, is frozen later
It is dry.
Embodiment 10
The medical super-hydrophobic antiseptic dressing and the difference of embodiment 1 are only that:
Step S1. buffers the Tris that the common medical absorbent cotton/gauze of 50mg is immersed in the PH=8.5 that 30ml concentration is 10mM
In liquid, 60mg dopamine hydrochloride is then added, reaction overnight 11h makes dopamine self-assemble on medical absorbent cotton/gauze, obtains
To by the first dressing sample of poly-dopamine modified lithium.
Embodiment 11
The medical super-hydrophobic antiseptic dressing and the difference of embodiment 10 are only that:
S3., second dressing sample is immersed in the AgNO of concentration 6mg/ml3In solution, 5h is reacted, makes silver ion poly- more
It is reduced to Nano silver grain on bar amine, the sample obtained after reaction is washed with deionized at least 2 times, is lyophilized later.
Reference examples 1
This reference examples provides a kind of antibacterial gauze dressing, is made by following methods:
S1. the common medical absorbent cotton/gauze of 50mg is immersed in the Tris buffer for the PH=8.5 that 30ml concentration is 10mM, is added
Enter 120mg dopamine, reaction overnight 12h is obtained by the first dressing sample of poly-dopamine modified lithium.
S2. the first dressing sample is washed with deionized 3 times, removes extra dopamine, and with dehydrated alcohol rinse.
Then the AgNO of concentration 8mg/ml is added3After reacting 5h in solution, the first sample is obtained.
S3. the first sample is immersed in volume ratio ethyl alcohol: mistake inside 1H, 1H, 2H, 2H- perfluor decyl mercaptan=1:500 solution
Night 12h, wherein 1H, 1H, 2H, 2H- perfluor decyl mercaptan alcohol mixture amount to 10ml, 1H, 1H, 2H, 20 μ of 2H- perfluor decyl mercaptan
l.The second sample obtained after reaction is washed with deionized 3 times, is lyophilized later.
Reference examples 2
This reference examples provides a kind of antibacterial gauze dressing, is made by following methods:
S1. the common medical absorbent cotton/gauze of 50mg is immersed in the Tris buffer for the PH=8.5 that 30ml concentration is 10mM, is added
Enter 120mg dopamine, reaction overnight 12h is obtained by the first dressing sample of poly-dopamine modified lithium.
S2. the first dressing sample is washed with deionized 3 times, removes extra dopamine, and with dehydrated alcohol rinse.
Then it is 8mg/mlAgNO that concentration, which is added,3React 5h in solution, obtained sample be washed with deionized 3 times, be placed on
Freeze-drying removes moisture under freezing environment.
Test example 1
The gauze dressing that simple vaseline, the clinical business wet silver of moral, embodiment 1, reference examples 1 and reference examples 2 are provided,
Water contact angle is measured with water contact angle measuring instrument, hydrophobicity experiment is done and compares, as shown in Figure 1, wherein A applies for petrolatum gauze
Material, B are the wet silver-colored gauze dressing of moral, and C is the medical super-hydrophobic antiseptic dressing that embodiment 1 provides, and D is the antibacterial that reference examples 1 provide
Gauze dressing, E are the antibacterial gauze dressing that control group 2 provides.
According to Fig. 1, the water contact angle of medical super-hydrophobic antiseptic dressing provided by the invention is 135.3 ± 1, and it is each to be greater than remaining
Group that is to say that its hydrophobicity is apparently higher than other groups.Wherein, reference examples 2 are without hydrophobicity.
Test example 2
It with CFU is diluted to is 10 with the staphylococcus aureus of meat soup culture7A bacterium takes the bacterium of 100 μ L to 96 orifice plates,
Addition is cut into uniform different gauze, 37 DEG C of insulating box culture 4h, after bacterium solution dilutes 40 times, takes 100 μ L in agar plate, and 37
DEG C culture 12h.Compare petrolatum gauze, the clinical business wet silver-colored gauze of moral, the embodiment of the present invention 1 provide it is medical super-hydrophobic anti-
The gauze dressing that the gauze dressing and reference examples 2 that bacterium dressing, reference examples 1 provide provide imitates the antibacterial of staphylococcus aureus
Fruit, as a result as shown in Figure 2.
According to fig. 2, it can be seen that medical super-hydrophobic antiseptic dressing provided by the invention has preferably anti-golden yellow grape
The effect of coccus.Wherein, test example 1 is due to first plus silver nitrate, then plus fluorine-bearing modifier, dredged although gauze dressing obtained has
It is aqueous, but anti-Staphylococcus aureus is ineffective, it may be possible to because the perfluor of grafting forms a tunic, protected silver from
Son prevents silver ion from discharging.
Test example 3
Wound healing situation after wounds in mice administration to the 14th day is described, the results are shown in Table 1.
Effect of each gauze dressing of table 1 to the surface of a wound
When mouse is administered the 4th, 7,10 day, the juice taken on every group of wounds in mice is wiped with aseptic cotton carrier respectively, with life
It manages it after salt water dilutes in being coated on agar plate, as a result as shown in figure 3, in conjunction with Fig. 3 and table 1, it is known that the 1st group and the 3rd
Bacterium amount significantly reduces the surface of a wound of group on day 4, and the bacterium amount in the 2nd group of the surface of a wound is always maintained at highest.
It is tested by the above wound healing it is found that medical super-hydrophobic antiseptic dressing produced by the present invention, antibiotic property are obviously big
In vaseline, hydrophobicity is significantly greater than the wet silver of moral.
Test example 4
Use identical method: Escherichia coli with meat soup culture are with being diluted to CFU as 107A bacterium takes the bacterium of 100 μ L
To 96 orifice plates, addition is cut into uniform different gauzes, 37 DEG C of insulating box culture 4h, after bacterium solution dilutes 40 times, take 100 μ L in
Agar plate, 37 DEG C of culture 12h.Compare reference examples 1, embodiment 1, reference examples 2, the clinical business wet silver-colored gauze of moral, vaseline gauze
Cloth for Escherichia coli antibacterial effect, as a result as shown in Figure 4.
Test example 5
The common medical absorbent cotton/gauze of 50mg is immersed in the PH=8.5Tris buffer that 30ml concentration is 10mM, is added not
With concentration dopamine 30mg, 60mg;Reaction overnight 12h.After 12h, manufactured dopamine gauze is washed with deionized 3 times,
Remove extra dopamine, and with dehydrated alcohol rinse.Various concentration AgNO is added in obtained sample3In solution, 5h is reacted,
Wherein AgNO3Concentration 0.5mg/ml, 1mg/ml;Different ethyl alcohol and fluorine-bearing modifier ratio 250:1,500:1,1000:1 are adjusted,
Sample obtained above is washed with deionized 3 times, be placed under freezing environment freeze 12h~36h after, freeze-drying remove water
Point.Compare various concentration dopamine, various concentration AgNO3Hydrophobic gauze, adjust different ethyl alcohol and fluorine-bearing modifier ratio
Contact angle, as a result as shown in Figure 5.Wherein, in Fig. 5,250,500 and 1000 cylindricality respectively refers to ethyl alcohol and fluorine-bearing modifier ratio
Example 250,500 and 1000.
Can be obtained according to Fig. 5, under conditions of ethyl alcohol and fluorine-bearing modifier ratio 250:1,500:1,1000:1, dopamine with
Under conditions of concentration proportion 1:0.5,1:1,1:2,2:2.05,2:1 and 2:2 of silver ion, medical super-hydrophobic antibacterial obtained
Dressing all has hydrophobicity.
To sum up, the preparation method of the medical super-hydrophobic antiseptic dressing of the offer of the embodiment of the present invention, it is easy to operate controllable, lead to
Crossing this method control dressing can sterilize in the different phase of wound healing to reach, the purpose of anti-adhesive.It is provided by the invention
Medical super-hydrophobic antiseptic dressing can be used as acute wound or infective wound surface dressing with preferable hydrophobicity and antibiotic property.
Embodiments described above is a part of the embodiment of the present invention, instead of all the embodiments.Reality of the invention
The detailed description for applying example is not intended to limit the range of claimed invention, but is merely representative of selected implementation of the invention
Example.Based on the embodiments of the present invention, obtained by those of ordinary skill in the art without making creative efforts
Every other embodiment, shall fall within the protection scope of the present invention.
Claims (10)
1. a kind of preparation method of medical super-hydrophobic antiseptic dressing, characterized in that it comprises:
Dressing and dopamine or DOPA amine salt are placed in alkalescent buffer and are made using the autohemagglutination of the dopamine by poly- more
The first modified dressing sample of bar amine;
First dressing sample is placed in the solution of fluorine-bearing modifier react the poly-dopamine modified lithium through fluorine modification
Two dressing samples;
Second dressing sample, which is placed in reaction in silver ion solution, is reduced the silver ion in the poly-dopamine
For Nano silver grain.
2. the preparation method of medical super-hydrophobic antiseptic dressing according to claim 1, which is characterized in that described to contain fluorine richness
Agent is 1H, 1H, 2H, 2H-perfluor decyl mercaptan, perfluor trimethylolpropane, perfluor polyethylene glycol, the fluoro- 1,4- benzene of 2,3,5,6- tetra-
At least one of dimethanol, perfluor-n-butyl alcohol, perfluor -1- octanol, 1,4- succinimide mercaptans and 1,6- ethanthiol;
Preferably, the fluorine-bearing modifier is 1H, 1H, 2H, 2H-perfluor decyl mercaptan.
3. the preparation method of medical super-hydrophobic antiseptic dressing according to claim 1, which is characterized in that described to contain fluorine richness
The solution of agent is 1H, 1H, 2H, 2H-perfluor decyl mercaptan ethanol solution, wherein the 1H, 1H, 2H, 2H-perfluor decyl mercaptan
Add the 0.025%-0.4% for the liquor capacity that volume is the fluorine-bearing modifier.
4. the preparation method of medical super-hydrophobic antiseptic dressing according to claim 1, which is characterized in that by the dressing with
The step that dopamine or DOPA amine salt are placed in alkaline buffer includes:
After the dressing is placed in alkaline buffer, dopamine or DOPA amine salt are added;
Preferably, the DOPA amine salt is dopamine hydrochloride.
5. the preparation method of medical super-hydrophobic antiseptic dressing according to claim 1, which is characterized in that the alkaline buffer
Liquid is the Tris buffer that pH value is 8-9;
Preferably, the concentration of addition of the dopamine in the Tris buffer is 3-4.5mg/ml;
Preferably, the dressing is placed in the alkaline buffer containing dopamine after reacting 10-15h, obtains first dressing
Sample.
6. the preparation method of medical super-hydrophobic antiseptic dressing according to claim 1, which is characterized in that silver ion solution choosing
From any one in silver nitrate solution, silver acetate solution and silver lactate solutions;
Preferably, the concentration of silver ion described in the silver ion solution is 7-16mg/ml;
Preferably, the silver ion solution is silver nitrate solution.
7. the preparation method of medical super-hydrophobic antiseptic dressing according to claim 1, which is characterized in that by the first dressing
Sample is placed in front of silver ion solution, further includes carrying out rinse to the first dressing sample, the solvent that the rinse uses is described
The solvent of the solution of fluorine-bearing modifier.
8. the preparation method of medical super-hydrophobic antiseptic dressing according to claim 1, which is characterized in that the dressing is selected from
Hospital gauze or medical non-woven fabrics.
9. a kind of medical super-hydrophobic antiseptic dressing, which is characterized in that medical super-hydrophobic as described in claim 1-8 any one
The preparation method of antiseptic dressing is made.
10. a kind of medical super-hydrophobic antiseptic dressing, which is characterized in that it includes dressing and antibacterial hydrophobic unit, and the antibacterial is dredged
Water unit through fluorine modification and doped with the poly-dopamine of Nano silver grain by constituting, and the antibacterial hydrophobic unit is through the poly- DOPA
Amine is attached to the fiber surface of the dressing.
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