CN109430514A - A method of preparing Tilapia mossambica-dregs of beans co-precipitation albumen - Google Patents
A method of preparing Tilapia mossambica-dregs of beans co-precipitation albumen Download PDFInfo
- Publication number
- CN109430514A CN109430514A CN201811298253.6A CN201811298253A CN109430514A CN 109430514 A CN109430514 A CN 109430514A CN 201811298253 A CN201811298253 A CN 201811298253A CN 109430514 A CN109430514 A CN 109430514A
- Authority
- CN
- China
- Prior art keywords
- dregs
- beans
- tilapia mossambica
- protein
- albumen
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/04—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from fish or other sea animals
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/001—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from waste materials, e.g. kitchen waste
- A23J1/005—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from waste materials, e.g. kitchen waste from vegetable waste materials
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Peptides Or Proteins (AREA)
Abstract
The present invention provides a kind of method for preparing Tilapia mossambica-dregs of beans and being co-precipitated albumen, it is characterized in that, the following steps are included: Tilapia and defatted soybean meal powder are mixed (in terms of butt) by the mass ratio of 1:5-5:1 by S1., with material: water=1:5-15 (g:mL) distilled water on the rocks；S2.10000-15000r/min homogeneous 1-5 min；S3. the pH of homogeneous mixture is adjusted to 3.0-8.0, stands 5min；S4. the cosolvent of 2% mass ratio is added, dissolution 5-30 min is stirred at room temperature；S5.4 DEG C, 10-20 min is centrifuged under 8000-11000 r/min；S6. supernatant is taken, adjustment pH precipitates supernatant, repeats step S5；S7. the precipitating after the centrifugation of collection step S5, S6, vacuum freeze drying obtain Tilapia mossambica-dregs of beans and are co-precipitated albumen.The present invention prepares novel " double protein " product, to improve fish protein dissolubility, emulsibility, gel characteristic etc., the novel double protein product that exploitation animal/vegetable protein is combined.
Description
Technical field
The invention belongs to aquatic product protein higher value application technical fields, and in particular to a kind of to prepare Tilapia mossambica-dregs of beans coprecipitated
The method of shallow lake albumen.
Background technique
China is Tilapia mossambica big producer, big trading nation, and consumption big country, and Tilapia mossambica outlet is mainly marketing fresh, and is produced
Product are to freeze Tilapia Fillet and freeze based on whole Tilapia mossambica.Primary processing accounts for major part in Tilapia mossambica converted products, and product is more single
One, profit is low, therefore restricts the development of Tilapia mossambica industry.In view of the above problems, intensive processing with improve Tilapia mossambica added value and
Profit margin.
Different types of albumen generally has Different Nutrition characteristic and functional characteristic, and the albumen for mixing separate sources can mention
The utilization of high high protein raw material overcomes the shortcomings of essential amino acids content in single source protein, nutritive value discord function
The problems such as energy characteristic is poor.The mixing of protein mainly includes that protein raw materials directly mix (physical mixed) and chemical mixing.Altogether
Protein precipitation (Co-precipitated protein, Co-PP) refers to using isoelectric precipitation, acid-thermoprecipitation or acid-heat
It induces and is aided with precipitating reagent such as CaCl2The methods of, a kind of mixed protein obtained in one or more kinds of raw materials.
The co-precipitation albumen of the protein preparation of separate sources, not only nutritionally may be implemented the complementation of amino acid, and
And there are good functional characteristic and ideal organoleptic attribute.It is huge that the above research illustrates that co-precipitation albumen has in the food industry
Potential application.
Currently, the novel product occurred in the market has surimi product, pickled product, frozen goods etc. using Tilapia mossambica as raw material.Sieve
Non- Fish protein is unstable in actual production process, is easily denaturalized, and dissolubility is poor, and then causes the structure of albumen
It changes with functional characteristic.This makes Tilapia mossambica protein gel characteristic and emulsifiability lower, influences the quality of product, limitation
Its application in field of food.Currently, being badly in need of a kind of quality for improving Rofe fishery -ies product, expand its application in field of food
Method improves the whole profit margin of Tilapia mossambica.
Summary of the invention
In view of this, the present invention utilizes the nutritional advantages of Rofe Fish protein and the functional characteristic advantage of soybean protein, with sieve
The non-flesh of fish and defatted soybean meal are raw material, adjust method preparation co-precipitation albumen with pH, novel " double protein " product are prepared, to improve fish
Meat albumen dissolubility, emulsibility, gel characteristic etc., the novel double protein product that exploitation animal/vegetable protein is combined.
The technical solution of the present invention is as follows: a kind of prepare Tilapia mossambica-dregs of beans co-precipitation albumen method, which is characterized in that packet
Include following steps:
S1. Tilapia and defatted soybean meal powder are mixed into (in terms of butt) by the mass ratio of 1:5-5:1, with material: water=1:5-15
(g:mL) distilled water on the rocks；
S2.10000-15000r/min homogeneous 1-5 min；
S3. the pH of homogeneous mixture is adjusted to 3.0-8.0, stands 5min；
S4. the cosolvent of 2% mass ratio is added, dissolution 5-30 min is stirred at room temperature；The mass ratio is sieve of cosolvent and addition
The ratio of the sum of non-fish and Soybean Meal.
S5. 4 DEG C, 10-20 min is centrifuged under 8000-11000 r/min；
S6. supernatant is taken, adjustment pH precipitates supernatant, repeats step S5；
S7. the precipitating after the centrifugation of collection step S5, S6, vacuum freeze drying obtain Tilapia mossambica-dregs of beans and are co-precipitated albumen.
It further, is by the pH 4.0-6.0 of homogeneous mixture in step S3.
It further, in step S6, is adjusted pH to 4.0-6.0.
Being deviateed in the acidity and alkaline pH range of isoelectric point by discovery, ascendant trend is presented in soluble protein yield, and
Yield is dissolved under the conditions of extreme 2.0,3.0,11.0,12.0 soda acid pH reaches maximum.This is because in acid range, part
The negative electrical charge of the residue branch carboxyl institute band of acidic amino acid is neutralized, so protein molecular has net positive charge；Alkaline model
In enclosing, deprotonation reaction occurs for the alkaline group of partial amino-acid, causes protein molecular with net negative charge, the hydration of albumen
Effect enhancing, protein solubility increase.The present invention by a large amount of creative test discovery, Tilapia and defatted soybean meal powder with
The soluble protein solution that mass ratio (butt meter) mixing is extracted is dissolved in pH 2.0,3.0,11.0 and 12.0, pH 4.0-6.0
When precipitate, precipitating yield be 88%-95%, it is preferred that pH 4.5 precipitate when yield highest, thereby determine that Tilapia mossambica-dregs of beans
The most suitable isoelectric point of acid/base soluble protein is 4.5.And for vegetable protein acid sink isoelectric pH it is generally relatively low, Rofe
After fish mixes with dregs of beans, the pH of the heavy isoelectric point of acid is partial to the isoelectric point of dregs of beans.Thus may determine that Tilapia mossambica-dregs of beans acid/base
The precipitating pH of soluble protein is 4.0-6.0, preferably pH 4.5.
It further, is that Tilapia and defatted soybean meal powder are mixed into (butt with the mass ratio of 1:2-2:1 in step S1
Meter), with material: water=1:7-10 (g:mL) distilled water on the rocks.The present invention is by a large amount of creative experiments, in conjunction with two kinds of different eggs
The characteristic of white matter obtains material-water ratio when optimal mixed proportion and dissolution, obtains optimal co-precipitation albumen preparation condition.
Further, the cosolvent includes following parts by weight component: lecithin 1-10, mannose 12-19, dioxy
SiClx 3-9, lysozyme 0.2-0.9, lysine 11-18, arginine 14-22, glutamic acid 7-16.In the present invention, addition
The effect of cosolvent is can to enhance its egg near isoelectric point during the formation of Tilapia mossambica-dregs of beans co-precipitation albumen
The reciprocation of white matter makes Rofe Fish protein further be combined to form stable co-precipitation protein structure with dregs of beans albumen, mentions
The yield of height co-precipitation albumen.
Specifically, facilitating protein renaturation in the environment of lysozyme, lysine, arginine, glutamic acid coexist, promote
Be properly fold into natural activity state into protein, avoid protein formed be co-precipitated albumen before occur in advance irreversible aggrengation and
Precipitating, it is ensured that the reciprocation of Rofe Fish protein and dregs of beans albumen in isoelectric point；Meanwhile lecithin, mannose, silica energy
Enough improve the homogeneity and stability of entire reaction system so that Rofe Fish protein and dregs of beans albumen near isoelectric point will not
There is undesirable reaction.
Further, the cosolvent includes following parts by weight component: lecithin 3-7, mannose 14-16, dioxy
SiClx 4-7, lysozyme 0.3-0.6, lysine 13-17, arginine 15-20, glutamic acid 9-14.
Further, the cosolvent includes following parts by weight component: lecithin 5, mannose 15, silica
5.5, lysozyme 0.4, lysine 14, arginine 17, glutamic acid 11.Particularly, the enzyme activity of the lysozyme is 100-
300U/g, it is preferred that the enzyme activity of the lysozyme is 150U/g.
The innovation of the invention consists in that: since Rofe Fish protein is unstable in actual production process, easily become
Property, and dissolubility is poor, and then the structural and functional characteristic of albumen is caused to change so that Tilapia mossambica protein gel characteristic and
Emulsibility is lower.The present invention is by combining the preferable dissolubility of soybean protein isolate, emulsion stability, foaminess and good sense
A kind of Tilapia mossambica-dregs of beans co-precipitation albumen is prepared in the advantages that official's characteristic, exploitation, so that fish protein dissolubility, emulsibility, solidifying
For glue characteristic etc. with improving well, the present invention has developed a kind of novel double protein product that animal/vegetable protein is combined.It is logical
Tilapia mossambica-dregs of beans co-precipitation albumen the yield for crossing invention preparation is high, and safety and stability expands for the quality for improving Rofe fishery -ies product
It provides a kind of effective way in the method for the application of field of food, the whole profit margin for improving Tilapia mossambica.
Specific embodiment
Technical solution of the present invention is clearly and completely described below in conjunction with embodiment, it is clear that described reality
Applying example is only a part of the embodiment of the present invention, instead of all the embodiments.
Embodiment 1
A method of preparing Tilapia mossambica-dregs of beans co-precipitation albumen, which comprises the following steps:
S1. Tilapia and defatted soybean meal powder are mixed into (in terms of butt) by the mass ratio of 1:5, with material: water=1:5 (g:mL) adds
Ice distilled water；
1 min of S2.10000r/min homogeneous；
S3. the pH of homogeneous mixture is adjusted to 3.0, stands 5min；
S4. the cosolvent of 2% mass ratio is added, dissolution 5min is stirred at room temperature；
S5. 4 DEG C, be centrifuged 10 min under 8000 r/min；
S6. supernatant is taken, adjustment pH precipitates supernatant, repeats step S5；
S7. the precipitating after the centrifugation of collection step S5, S6, vacuum freeze drying obtain Tilapia mossambica-dregs of beans and are co-precipitated albumen.
It further, in step S6, is adjusted pH to 4.5.
Further, the cosolvent includes following parts by weight component: lecithin 1, mannose 12, silica 3,
150U/g lysozyme 0.2, lysine 11, arginine 14, glutamic acid 7.In the present invention, the effect of the cosolvent of addition is
The reciprocation of its protein near isoelectric point can be enhanced during the formation of Tilapia mossambica-dregs of beans co-precipitation albumen,
Make Rofe Fish protein further be combined to form stable co-precipitation protein structure with dregs of beans albumen, improves obtaining for co-precipitation albumen
Rate.
Embodiment 2
A method of preparing Tilapia mossambica-dregs of beans co-precipitation albumen, which comprises the following steps:
S1. Tilapia and defatted soybean meal powder are mixed into (in terms of butt) by the mass ratio of 5:1, with material: water=1:15 (g:mL)
Distilled water on the rocks；
5 min of S2.15000r/min homogeneous；
S3. the pH of homogeneous mixture is adjusted to 8.0, stands 5min；
S4. the cosolvent of 2% mass ratio is added, 30 min of dissolution are stirred at room temperature；
S5. 4 DEG C, be centrifuged 20 min under 11000 r/min；
S6. supernatant is taken, adjustment pH precipitates supernatant, repeats step S5；
S7. the precipitating after the centrifugation of collection step S5, S6, vacuum freeze drying obtain Tilapia mossambica-dregs of beans and are co-precipitated albumen.
It further, in step S6, is adjusted pH to 4.5.
Further, the cosolvent includes following parts by weight component: lecithin 10, mannose 19, silica
9,150U/g lysozyme 0.9, lysine 18, arginase 12 2, glutamic acid 16.In the present invention, the effect of the cosolvent of addition
It is that can enhance its interaction of protein near isoelectric point during the formation of Tilapia mossambica-dregs of beans co-precipitation albumen and make
With, make Rofe Fish protein further be combined to form stable co-precipitation protein structure with dregs of beans albumen, improve co-precipitation albumen
Yield.
Embodiment 3
A method of preparing Tilapia mossambica-dregs of beans co-precipitation albumen, which comprises the following steps:
S1. Tilapia and defatted soybean meal powder are mixed into (in terms of butt) by the mass ratio of 1:2, with material: water=1:7 (g:mL)
Distilled water on the rocks；
2.5 min of S2.10000r/min homogeneous；
S3. the pH of homogeneous mixture is adjusted to 4.0, stands 5min；
S4. the cosolvent of 2% mass ratio is added, 10 min of dissolution are stirred at room temperature；
S5. 4 DEG C, be centrifuged 12min under 9000 r/min；
S6. supernatant is taken, adjustment pH precipitates supernatant, repeats step S5；
S7. the precipitating after the centrifugation of collection step S5, S6, vacuum freeze drying obtain Tilapia mossambica-dregs of beans and are co-precipitated albumen.
It further, in step S6, is adjusted pH to 4.0.
Further, the cosolvent includes following parts by weight component: lecithin 3, mannose 14, silica 4,
150U/g lysozyme 0.3, lysine 13, arginine 15, glutamic acid 9.
Embodiment 4
A method of preparing Tilapia mossambica-dregs of beans co-precipitation albumen, which comprises the following steps:
S1. Tilapia and defatted soybean meal powder are mixed into (in terms of butt) by the mass ratio of 2:1, with material: water=1:10 (g:mL)
Distilled water on the rocks；
3.5 min of S2.13000r/min homogeneous；
S3. the pH of homogeneous mixture is adjusted to 6.0, stands 5min；
S4. the cosolvent of 2% mass ratio is added, 25 min of dissolution are stirred at room temperature；
S5. 4 DEG C, be centrifuged 17 min under 10000 r/min；
S6. supernatant is taken, adjustment pH precipitates supernatant, repeats step S5；
S7. the precipitating after the centrifugation of collection step S5, S6, vacuum freeze drying obtain Tilapia mossambica-dregs of beans and are co-precipitated albumen.
It further, in step S6, is adjusted pH to 6.0.
Further, the cosolvent includes following parts by weight component: lecithin 7, mannose 16, silica 7,
150U/g lysozyme 0.6, lysine 17, arginase 12 0, glutamic acid 14.
Embodiment 5
A method of preparing Tilapia mossambica-dregs of beans co-precipitation albumen, which comprises the following steps:
S1. Tilapia and defatted soybean meal powder are mixed into (in terms of butt) by the mass ratio of 2:3, with material: water=1:9 (g:mL) adds
Ice distilled water；
2 min of S2.12000r/min homogeneous；
S3. the pH of homogeneous mixture is adjusted to 4.2, stands 5min；
S4. the cosolvent of 2% mass ratio is added, 20 min of dissolution are stirred at room temperature；
S5. 4 DEG C, be centrifuged 15 min under 9500 r/min；
S6. supernatant is taken, adjustment pH precipitates supernatant, repeats step S5；
S7. the precipitating after the centrifugation of collection step S5, S6, vacuum freeze drying obtain Tilapia mossambica-dregs of beans and are co-precipitated albumen.
It further, in step S6, is adjusted pH to 4.5.
Further, the cosolvent includes following parts by weight component: lecithin 5, mannose 15, silica
5.5,150U/g lysozyme 0.4, lysine 14, arginine 17, glutamic acid 11.
Comparative example 1
A method of preparing Tilapia mossambica-dregs of beans co-precipitation albumen, which comprises the following steps:
S1. Tilapia and defatted soybean meal powder are mixed into (in terms of butt) by the mass ratio of 2:3, with material: water=1:9 (g:mL) adds
Ice distilled water；
2 min of S2.12000r/min homogeneous；
S3. the pH of homogeneous mixture is adjusted to 4.2, stands 5min；
S4. the cosolvent of 2% mass ratio is added, 20 min of dissolution are stirred at room temperature；
S5. 4 DEG C, be centrifuged 15 min under 9500 r/min；
S6. supernatant is taken, adjustment pH precipitates supernatant, repeats step S5；
S7. the precipitating after the centrifugation of collection step S5, S6, vacuum freeze drying obtain Tilapia mossambica-dregs of beans and are co-precipitated albumen.
It further, in step S6, is adjusted pH to 4.5.
Further, the cosolvent includes following parts by weight component: lecithin 5, mannose 15, silica
5.5。
Comparative example 2
A method of preparing Tilapia mossambica-dregs of beans co-precipitation albumen, which comprises the following steps:
S1. Tilapia and defatted soybean meal powder are mixed into (in terms of butt) by the mass ratio of 2:3, with material: water=1:9 (g:mL) adds
Ice distilled water；
2 min of S2.12000r/min homogeneous；
S3. the pH of homogeneous mixture is adjusted to 4.2, stands 5min；
S4. the cosolvent of 2% mass ratio is added, 20 min of dissolution are stirred at room temperature；
S5. 4 DEG C, be centrifuged 15 min under 9500 r/min；
S6. supernatant is taken, adjustment pH precipitates supernatant, repeats step S5；
S7. the precipitating after the centrifugation of collection step S5, S6, vacuum freeze drying obtain Tilapia mossambica-dregs of beans and are co-precipitated albumen.
It further, in step S6, is adjusted pH to 4.5.
Further, the cosolvent includes following parts by weight component: 150U/g lysozyme 0.4, lysine 14,
Arginine 17, glutamic acid 11.
Comparative example 3
A method of preparing Tilapia mossambica-dregs of beans co-precipitation albumen, which comprises the following steps:
S1. Tilapia and defatted soybean meal powder are mixed into (in terms of butt) by the mass ratio of 2:3, with material: water=1:9 (g:mL) adds
Ice distilled water；
2 min of S2.12000r/min homogeneous；
S3. the pH of homogeneous mixture is adjusted to 4.2, stands 5min；
S4. 20 min of dissolution are stirred at room temperature；
S5. 4 DEG C, be centrifuged 15 min under 9500 r/min；
S6. supernatant is taken, adjustment pH precipitates supernatant, repeats step S5；
S7. the precipitating after the centrifugation of collection step S5, S6, vacuum freeze drying obtain Tilapia mossambica-dregs of beans and are co-precipitated albumen.
It further, in step S6, is adjusted pH to 4.5.
Experiment test
It is co-precipitated albumen yield
SDS-PAGE electrophoretic analysis is carried out to the supernatant of embodiment 1-5 and comparative example 1-3, obtains containing for soluble protein
Amount obtains protein precipitation yield, is as co-precipitated the thick yield of albumen by deducting the content of soluble protein.Using separation
Gum concentration is 12%, concentration gum concentration is 5% carry out SDS-PAGE electrophoresis.Sample supernatant and SDS-PAGE loading buffer liquid phase are mixed
It closes (4:1, v/v), boils 6 min before electrophoresis tank is added, i.e. loading volume is 10 μ L in equal volume, bright with R-250 coomassie
Indigo plant is dyed, and decoloration uses the acetum of high methanol, i.e., plus then the acetic acid of 75 mL, 50 mL methanol are settled to 1 with water
L.As a result as shown in the table:
。
It is co-precipitated protein emulsifying performance test
Protein concentration 0.5% is configured, using the emulsifying activity (EA) and emulsion stability (ES) of Nephelometric Determination albumen.Take 6 mL
In 50 mL small beakers the golden dragonfish level-one soybean oil of 2 mL is added, with 1 min(fixed rotating speed of homogenizer homogeneous in sample
12000 r/min).With it is ultraviolet under 500 nm wavelength using 0.1% SDS as blank, take 20 from beaker bottom rapidlyμL emulsus
Liquid point is dissolved in the SDS solution of 4 mL 0.1%, is shaken up, measure 0 min absorbance value (A 0)；20 are taken again after standing 10 minμL emulsion measurement absorbance value (A 10).EA with emulsifying activity index (EAI) indicate, ES with stable emulsifying sex index (ESI) come
It indicates.Formula is as follows:
；
；
Wherein:DFor extension rate, D=200；CFor protein concentration,C=0.5%(g/mL)；ΦFor oil volumetric fraction,Φ=1/4；LFor the thickness of cuvette,L=1 cm；△t=10 min.As a result as shown in the table:
。
It is obvious to a person skilled in the art that invention is not limited to the details of the above exemplary embodiments, Er Qie
In the case where without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, the present embodiments are to be considered as illustrative and not restrictive, and the scope of the present invention is by appended power
Benefit requires rather than above description limits, it is intended that all by what is fallen within the meaning and scope of the equivalent elements of the claims
Variation is included within the present invention.
In addition, it should be understood that although this specification is described in terms of embodiments, but not each embodiment is only wrapped
Containing an independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should
It considers the specification as a whole, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
The other embodiments being understood that.It is noted that the technical characteristic being not described in detail in the present invention, can pass through this
Field any prior art is realized.
Claims (7)
1. a kind of prepare Tilapia mossambica-dregs of beans co-precipitation albumen method, which comprises the following steps:
S1. Tilapia and defatted soybean meal powder are mixed into (in terms of butt) by the mass ratio of 1:5-5:1, with material: water=1:5-15
(g:mL) distilled water on the rocks；
S2.10000-15000r/min homogeneous 1-5 min；
S3. the pH of homogeneous mixture is adjusted to 3.0-8.0, stands 5min；
S4. the cosolvent of 2% mass ratio is added, dissolution 5-30 min is stirred at room temperature；
S5. 4 DEG C, 10-20 min is centrifuged under 8000-11000 r/min；
S6. supernatant is taken, adjustment pH precipitates supernatant, repeats step S5；
S7. the precipitating after the centrifugation of collection step S5, S6, vacuum freeze drying obtain Tilapia mossambica-dregs of beans and are co-precipitated albumen.
2. preparation Tilapia mossambica according to claim 1-dregs of beans co-precipitation albumen method, which is characterized in that step S3
In, it is by the pH 4.0-6.0 of homogeneous mixture.
3. preparation Tilapia mossambica according to claim 1-dregs of beans co-precipitation albumen method, which is characterized in that in step S6,
It is to adjust pH to 4.0-6.0.
4. preparation Tilapia mossambica according to claim 1-dregs of beans co-precipitation albumen method, which is characterized in that in step S1,
It is that Tilapia and defatted soybean meal powder are mixed into (in terms of butt) by the mass ratio of 1:2-2:1, with material: water=1:7-10 (g:mL)
Distilled water on the rocks.
5. preparation Tilapia mossambica according to claim 1-dregs of beans co-precipitation albumen method, which is characterized in that the hydrotropy
Agent includes following parts by weight component: lecithin 1-10, mannose 12-19, silica 3-9, lysozyme 0.2-0.9, being relied
Propylhomoserin 11-18, arginine 14-22, glutamic acid 7-16.
6. preparation Tilapia mossambica according to claim 5-dregs of beans co-precipitation albumen method, which is characterized in that the hydrotropy
Agent includes following parts by weight component: lecithin 3-7, mannose 14-16, silica 4-7, lysozyme 0.3-0.6, being relied
Propylhomoserin 13-17, arginine 15-20, glutamic acid 9-14.
7. preparation Tilapia mossambica according to claim 6-dregs of beans co-precipitation albumen method, which is characterized in that the hydrotropy
Agent includes following parts by weight component: lecithin 5, mannose 15, silica 5.5, lysozyme 0.4, lysine 14, essence
Propylhomoserin 17, glutamic acid 11.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811298253.6A CN109430514B (en) | 2018-11-02 | 2018-11-02 | Method for preparing tilapia mossambica-soybean meal coprecipitation protein |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811298253.6A CN109430514B (en) | 2018-11-02 | 2018-11-02 | Method for preparing tilapia mossambica-soybean meal coprecipitation protein |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109430514A true CN109430514A (en) | 2019-03-08 |
| CN109430514B CN109430514B (en) | 2022-07-26 |
Family
ID=65550558
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811298253.6A Active CN109430514B (en) | 2018-11-02 | 2018-11-02 | Method for preparing tilapia mossambica-soybean meal coprecipitation protein |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109430514B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116439314A (en) * | 2023-04-12 | 2023-07-18 | 东北农业大学 | Method for improving protein emulsification properties by preparing soybean-phycocyanin |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004041848A1 (en) * | 2002-11-04 | 2004-05-21 | Proteome Systems Intellectual Property Pty Ltd | Improved extraction of a proteinaceous macromolecule from a biological sample |
| CN1575298A (en) * | 2001-10-22 | 2005-02-02 | 多姆普公司 | Supercritical fluids processing: preparation of protein microparticles and their stabilisation |
| TW200722435A (en) * | 2005-04-11 | 2007-06-16 | Savient Pharmaceuticals Inc | Purification of proteins with cationic surfactant |
| CN101317623A (en) * | 2008-07-04 | 2008-12-10 | 华南理工大学 | Preparation method for oil-in-water type soybean isolated protein |
| CN102613382A (en) * | 2012-04-10 | 2012-08-01 | 青岛海健堂生物科技有限公司 | Fish collagen granular formulation and preparation method thereof |
-
2018
- 2018-11-02 CN CN201811298253.6A patent/CN109430514B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1575298A (en) * | 2001-10-22 | 2005-02-02 | 多姆普公司 | Supercritical fluids processing: preparation of protein microparticles and their stabilisation |
| WO2004041848A1 (en) * | 2002-11-04 | 2004-05-21 | Proteome Systems Intellectual Property Pty Ltd | Improved extraction of a proteinaceous macromolecule from a biological sample |
| TW200722435A (en) * | 2005-04-11 | 2007-06-16 | Savient Pharmaceuticals Inc | Purification of proteins with cationic surfactant |
| CN101317623A (en) * | 2008-07-04 | 2008-12-10 | 华南理工大学 | Preparation method for oil-in-water type soybean isolated protein |
| CN102613382A (en) * | 2012-04-10 | 2012-08-01 | 青岛海健堂生物科技有限公司 | Fish collagen granular formulation and preparation method thereof |
Non-Patent Citations (5)
| Title |
|---|
| HAGAN, RC ET AL: "TEXTURIZATION OF CO-PRECIPITATED SOYBEAN AND PEANUT PROTEINS BY TWIN-SCREW EXTRUSION", 《JOURNAL OF FOOD SCIENCE》 * |
| 付苇娅: "氨基酸诱导低离子强度下罗非鱼肌球蛋白增溶行为及机制", 《中国优秀硕士学位论文全文数据库（电子期刊） 工程科技1辑》 * |
| 代振清等: "罗非鱼蛋白-大豆蛋白共沉淀物的乳化性", 《食品与发酵工业》 * |
| 柴莎莎: "不同人群所需蛋白粉的研究", 《中国优秀硕士论文全文数据库 工程科技Ⅰ辑》 * |
| 齐慧红等: "罗非鱼-豆粕共沉淀蛋白的提取工艺及蛋白组成分析", 《食品工业科技》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116439314A (en) * | 2023-04-12 | 2023-07-18 | 东北农业大学 | Method for improving protein emulsification properties by preparing soybean-phycocyanin |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109430514B (en) | 2022-07-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Chen et al. | Physicochemical and functional properties of proteins extracted from three microalgal species | |
| Bashir et al. | Functional properties and amino acid profile of Spirulina platensis protein isolates | |
| CN1286389C (en) | Highly soluble, high molecular weight soy bean protein | |
| CN113017095B (en) | Preparation method and application of corn oil quinoa protein pickering high internal phase emulsion | |
| CN101427728B (en) | Method for improving wheat plantule protein capability with supersonic wave | |
| Ding et al. | Effect of ultrasonic treatment on the structure and functional properties of mantle proteins from scallops (Patinopecten yessoensis) | |
| CN105661474B (en) | Plant animal protein crosslinking recombination gel sauce production method | |
| CA2615854A1 (en) | Calcium containing soy protein isolate composition | |
| Yoon et al. | Chemical composition and functional properties of roe concentrates from skipjack tuna (Katsuwonus pelamis) by cook‐dried process | |
| KR920001702B1 (en) | Hydrolyzed protein iosolate & process of producing | |
| CN103082081A (en) | Yeast protein and preparation method thereof, food prepared from the protein as raw material and preparation method thereof | |
| CN105018558B (en) | A kind of clover multiple target coproduction extraction separation method | |
| CN103989152A (en) | Preparation method of needle mushroom hydrolysate | |
| Wang et al. | Improved backward extraction of walnut protein using AOT reverse micelles with microwave and its characteristics | |
| CN109430514A (en) | A method of preparing Tilapia mossambica-dregs of beans co-precipitation albumen | |
| Deng et al. | Chinese quince seed proteins: sequential extraction processing and fraction characterization | |
| Wu et al. | Physicochemical, structural, and functional properties of protein fractions and protein isolate from jackfruit seeds | |
| CN104957357B (en) | A kind of method that wet heating glycosylation modification improves ovalbumin emulsibility | |
| CN106636281A (en) | Enzymolysis method of sea bass byproduct and application thereof in emulsion product | |
| Tu et al. | Critical functional properties of defatted peanut meal produced by aqueous extraction and conventional methods | |
| Wang et al. | Physicochemical properties of seed protein isolates extracted from pepper meal by pressure-assisted and conventional solvent defatting | |
| CN110407917A (en) | A kind of octopus oligopeptides and the preparation method and application thereof promoting the synthesis of mammary gland casein | |
| Fu et al. | Physicochemical and functional properties of the muscle protein fraction of Hypomesus olidus | |
| James et al. | Chemical composition and functional properties of protein concentrate from selected cowpea seeds in Nigeria | |
| Wang et al. | Effects of selenium yeast and jujube powder dietary supplements on conformational and functional properties of post-mortem chicken myofibrillar protein |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |