CN109422812A - Reagent is used in monoclonal antibody preparation - Google Patents
Reagent is used in monoclonal antibody preparation Download PDFInfo
- Publication number
- CN109422812A CN109422812A CN201710772006.4A CN201710772006A CN109422812A CN 109422812 A CN109422812 A CN 109422812A CN 201710772006 A CN201710772006 A CN 201710772006A CN 109422812 A CN109422812 A CN 109422812A
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- China
- Prior art keywords
- solution
- culture solution
- monoclonal antibody
- tri
- distilled water
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Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/44—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material not provided for elsewhere, e.g. haptens, metals, DNA, RNA, amino acids
Abstract
The present invention provides the monoclonal antibody preparation reagents in technical field of biology, including Pidolidone solution, dual anti-solution and cell culture, HAT culture solution, HT culture solution, cell freezing liquid, guanozola storing liquid and PEG(50%), cell culture fluid includes endless full nutrient solution and complete culture solution；Pidolidone solution is made of glutamic acid and tri-distilled water, dual anti-solution is made of penicillin, streptomysin and tri-distilled water, HAT culture solution is made of complete culture solution and HAT, HT culture solution is made of complete culture solution and HT, cells frozen storing liquid is made of complete culture solution and DMSO, guanozola storing liquid is made of guanozola, sodium hydroxide solution and tri-distilled water, PEG(50%) it is made of PEG and endless full nutrient solution.
Description
Technical field
The present invention relates to a kind of additive, in particular to reagent is used in monoclonal antibody preparation.
Background technique
It is a variety of that OTA is widely present in Cereals class, raisins, cocoa, chocolate, coffee, grape wine, condiment, dairy products etc.
It in food and feed, can be entered in humans and animals body by food chain, there is cumulative toxicity.The pollution of OTA is more in feed
Seriously, animal takes in ota by contaminated feed of searching for food.Largely pass through the study found that nonruminant takes in intracorporal OTA
Blood is transferred to kidney, and being proximally and distally absorbed in alimentary canal and renal tubule, and OTA is divided repeatedly by hepato-enteric circulation
It secretes and absorbs, OTA is converted to avirulent metabolite OT α by the microbes positioned at enteron aisle；And enter blood OTA with
HAS combines closely, highly stable in animal body, is not easy to be metabolized degradation, therefore the kidney of animal food, especially pig,
Liver, muscle, blood etc. often have OTA detection.For, there may be OTA, need to prepare in human body or animal body a kind of reagent with
Develop monoclonal antibody.
Summary of the invention
For the defects in the prior art, it is an object of the invention to overcome the technology of the above-mentioned pollution of OTA in the prior art
Problem provides monoclonal antibody preparation reagent, and the present invention is that preparation monoclonal antibody is prepared, and reduces OTA.
The object of the present invention is achieved like this: monoclonal antibody preparation reagent, including Pidolidone solution, dual anti-molten
Liquid and cell culture, HAT culture solution, HT culture solution, cell freezing liquid, guanozola storing liquid and PEG(50%), it is described
Cell culture fluid includes endless full nutrient solution and complete culture solution；The Pidolidone solution is made of glutamic acid and tri-distilled water,
Dual anti-solution is made of penicillin, streptomysin and tri-distilled water, and the HAT culture solution is made of complete culture solution and HAT, the HT
Culture solution is made of complete culture solution and HT, and the cells frozen storing liquid is made of complete culture solution and DMSO, the 8- azepine bird
Purine storing liquid is made of guanozola, sodium hydroxide solution and tri-distilled water, the PEG(50%) by PEG and not exclusively training
Nutrient solution is made.
As a further improvement of the present invention, in the Pidolidone solution, glutamic acid 2.9g, tri-distilled water 100mL.
As a further improvement of the present invention, in the dual anti-solution, penicillin be ten thousand unit of G100, streptomysin 1g,
Tri-distilled water is 200mL.
As a further improvement of the present invention, the endless full nutrient solution is by 98ML DREM culture solution stoste, 1mL L-G
Solution and the dual anti-solution of 1mL are made；The complete culture solution is dual anti-by 78ML DREM culture solution stoste, 1mL L-G solution, 1mL
Solution and 20mL fetal calf serum are made.
As a further improvement of the present invention, in the HAT culture solution, complete culture solution 99mL, HAT 1mL.
As a further improvement of the present invention, in the HT culture solution, complete culture solution 99mL, HT 1mL.
As a further improvement of the present invention, in the cells frozen storing liquid, complete culture solution 90mL, DMSO 10mL.
As a further improvement of the present invention, in the guanozola storing liquid, guanozola 200mg,
Tri-distilled water is 100mL.
As a further improvement of the present invention, the PEG(50%) in, PEG 2g, endless full nutrient solution is 2mL.
Specific embodiment
Monoclonal antibody preparation reagent, including Pidolidone solution, dual anti-solution and cell culture, HAT culture solution, HT
Culture solution, cell freezing liquid, guanozola storing liquid and PEG(50%), cell culture fluid includes endless full nutrient solution and complete
Full nutrient solution；Pidolidone solution is made of glutamic acid and tri-distilled water, and dual anti-solution is by penicillin, streptomysin and tri-distilled water system
At HAT culture solution is made of complete culture solution and HAT, and HT culture solution is made of complete culture solution and HT, and cells frozen storing liquid is by complete
Full nutrient solution and DMSO are made, and guanozola storing liquid is made of guanozola, sodium hydroxide solution and tri-distilled water,
PEG(50% it) is made of PEG and endless full nutrient solution.
In Pidolidone solution, glutamic acid 2.9g, tri-distilled water 100mL, specific preparation method is 2.9g glutamic acid
It is dissolved in the tri-distilled water of 100mL, filtration sterilization dispenses bottle, and -20 DEG C freeze；In dual anti-solution, penicillin is ten thousand unit of G100, chain
Mycin is 1g, and tri-distilled water 200mL, specific preparation method is that 1,000,000 unit of benzyl penicillin and streptomysin 1g are dissolved in 200mL tri-
It steams in water, -20 DEG C freeze；Endless full nutrient solution is by 98ML DREM culture solution stoste, 1mL L-G solution and the dual anti-solution system of 1mL
At；Complete culture solution is by 78ML DREM culture solution stoste, 1mL L-G solution, the dual anti-solution of 1mL and 20mL fetal calf serum system
At；In HAT culture solution, complete culture solution 99mL, HAT 1mL；In HT culture solution, complete culture solution 99mL, HT 1mL；
In cells frozen storing liquid, complete culture solution 90mL, DMSO 10mL；In guanozola storing liquid, guanozola is
200mg, tri-distilled water 100mL, specific preparation method are guanozola with sodium hydroxide hydrotropy, are settled to tri-distilled water
100mL freezes, and is added when use according to 1% concentration；PEG(50% in), PEG 2g, endless full nutrient solution is 2mL, is specifically matched
Method processed is PEG 1450 after 8 pounds of sterilizing 15min, is added in the incomplete culture solution of preheating, mixes, 4 DEG C of storages.
Claims (9)
1. monoclonal antibody preparation reagent, it is characterised in that: including Pidolidone solution, dual anti-solution and cell culture, HAT
Culture solution, HT culture solution, cell freezing liquid, guanozola storing liquid and PEG(50%), the cell culture fluid includes not
Complete culture solution and complete culture solution；The Pidolidone solution is made of glutamic acid and tri-distilled water, dual anti-solution by penicillin,
Streptomysin and tri-distilled water are made, and the HAT culture solution is made of complete culture solution and HAT, and the HT culture solution by cultivating completely
Liquid and HT are made, and the cells frozen storing liquid is made of complete culture solution and DMSO, and the guanozola storing liquid is by 8- nitrogen
Miscellaneous guanine, sodium hydroxide solution and tri-distilled water are made, the PEG(50%) it is made of PEG and endless full nutrient solution.
2. monoclonal antibody preparation reagent according to claim 1, it is characterised in that: in the Pidolidone solution,
Glutamic acid is 2.9g, tri-distilled water 100mL.
3. monoclonal antibody preparation reagent according to claim 1, it is characterised in that: in the dual anti-solution, mould
Element is ten thousand unit of G100, streptomysin 1g, tri-distilled water 200mL.
4. monoclonal antibody according to claim 1 preparation reagent, it is characterised in that: the endless full nutrient solution by
98ML DREM culture solution stoste, 1mL L-G solution and the dual anti-solution of 1mL are made；The complete culture solution is trained by 78ML DREM
Nutrient solution stoste, 1mL L-G solution, the dual anti-solution of 1mL and 20mL fetal calf serum are made.
5. monoclonal antibody preparation reagent according to claim 1, it is characterised in that: in the HAT culture solution, completely
Culture solution is 99mL, HAT 1mL.
6. monoclonal antibody preparation reagent according to claim 1, it is characterised in that: in the HT culture solution, completely
Culture solution is 99mL, HT 1mL.
7. monoclonal antibody preparation reagent according to claim 1, it is characterised in that: complete in the cells frozen storing liquid
Full nutrient solution is 90mL, DMSO 10mL.
8. monoclonal antibody preparation reagent according to claim 1, it is characterised in that: the guanozola storage
In liquid, guanozola 200mg, tri-distilled water 100mL.
9. monoclonal antibody preparation reagent according to claim 1, it is characterised in that: the PEG(50%) in, PEG is
2g, endless full nutrient solution are 2mL.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710772006.4A CN109422812A (en) | 2017-08-31 | 2017-08-31 | Reagent is used in monoclonal antibody preparation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710772006.4A CN109422812A (en) | 2017-08-31 | 2017-08-31 | Reagent is used in monoclonal antibody preparation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109422812A true CN109422812A (en) | 2019-03-05 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710772006.4A Pending CN109422812A (en) | 2017-08-31 | 2017-08-31 | Reagent is used in monoclonal antibody preparation |
Country Status (1)
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| CN (1) | CN109422812A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP4042868A4 (en) * | 2019-10-08 | 2023-10-25 | Iwatani Corporation | Composition for freezing cells, method for freezing cells, method for culturing cells, and kit for freezing cells |
-
2017
- 2017-08-31 CN CN201710772006.4A patent/CN109422812A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP4042868A4 (en) * | 2019-10-08 | 2023-10-25 | Iwatani Corporation | Composition for freezing cells, method for freezing cells, method for culturing cells, and kit for freezing cells |
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| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20190305 |