CN109411026A - A kind of normal form using sludge function enzyme response early warning sewage disposal system early stage risk - Google Patents
A kind of normal form using sludge function enzyme response early warning sewage disposal system early stage risk Download PDFInfo
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Abstract
The present invention provides a kind of normal form using sludge function enzyme response early warning sewage disposal system early stage risk, and sludge function enzyme responds the difference of active emerging pollutant during being related to municipal sewage treatment, the early stage risk of early warning sewage disposal system.The sensibility that the present invention passes through Biological indicators and chemical index in comparison activated sludge, it can quickly early warning Sludge Property change or abnormal normal form to provide one, this method assessment result accuracy is higher, can stable sewage treatment to a certain extent efficiency, municipal wastewater treatment plant biological treatment system is reduced because Sludge Property causes to be discharged risk not up to standard extremely, the chemical index that sewage disposal system faces is made up to a certain extent and monitors brought limitation, improves business risk prevention ability.
Description
Technical field
The present invention relates to difference of the sludge function enzyme during municipal sewage treatment to active emerging pollutant to ring
It answers, the early stage risk of early warning sewage disposal system.
Background technique
With the increasingly increase of municipal sewage treatment amount, the unexpected thing that activated sludge process in sewage treatment may occur
Therefore processing becomes particularly important.Since each pollutant is mixed in water body, occur physically or chemically to make between pollutant
With generating new noxious material and cause secondary pollution to water body, effluent quality is caused to decline, the safety for influencing every water is asked
Topic, which results in the extensive concerns of the public and scientific circles.Existing method of environmental monitoring remains in conventional, traditional chemistry and refers to
Level monitoring is marked, the advanced means that chemical, biological multi objective interpenetrates are lacked to the sewage disposal system analysis of complicated components,
It has become unfavorable stress change induced organic pollutant (EOCs) and implements the bottle effectively supervised and controlled with ecology influence
Neck problem.Therefore, measure and predict influence of the different classes of EOCs to sewerage ecological system and sludge microbe group, screening shadow
The sensitive indicator for ringing sludge ecological functions facilitates to the greatest extent to the potential risk of sewage disposal system in the presence of scientific evaluation EOCs
There is the case where unusual fluctuation in early discovery and early warning sewage disposal system sludge activity.
It is still relatively fewer for the research of active sludge microorganism enzyme at home, therefore have very big application prospect.With
Research deepens continuously, and Microbial Communities in Activated Sludge enzyme can preferably be recognized by people, for preferably dirty using activity in the future
Mud has important scientific guidance meaning.Influence based on the pollutant of various concentration to microbial enzymatic activities surveys method using enzyme
Evaluation Controlling model is established, implements dynamic risk assessment in controlled range to form the exposure normal form of system, including
Theoretical basis and practice operation, to reach early warning and the preventive effect of risk.
Summary of the invention
What is used since sewage treatment plant is most of at present is all chemical index to monitor the operation conditions of sludge.To make up
The deficiency of conventional method, the present invention propose that Biological indicators are substantially better than chemical index to the sensibility of pollutant in activated sludge,
Providing one can quickly early warning Sludge Property change or abnormal normal form, and the activity of sensitive function enzyme is for being discharged water
Matter can play better indicative function, and for stablizing sewage treating efficiency, improving business risk prevention ability has important meaning
Justice.
To achieve the above object, the present invention adopts the following technical scheme:
S1. sample constant temperature incubation 24 hours in 37 DEG C of incubator are taken, aerobic sludge function enzymatic activity is analyzed,
Urease activity and invertase activity are calculated according to formula (1) and formula (2)；
Urease activity=(aSample-aWithout sludge-aWithout matrix)·nV/m； (1)
Invertase activity=(a 'Sample-a’Without sludge-a’Without matrix)·n/m； (2)
In formula, aSample, a 'SampleThe glucose in milligrams number or NH of standard curve calculating are corresponded to according to absorbance value for sample3-N
Milligram number；
aWithout sludge, a 'Without sludgeThe glucose in milligrams number or NH of standard curve calculating are corresponded to according to absorbance value for no sludge3- N milli
Grams；
aWithout matrix, a 'Without matrixThe glucose in milligrams number or NH of standard curve calculating are corresponded to according to absorbance value for no matrix3- N milli
Grams；
N is to divide to take multiple；
M is drying sludge quality/g；
V is developing solution volume/mL；
S2. corresponding dynamic variation of enzyme activity rate is calculated separately by the resulting urease activity of S1, invertase activity；
Dynamic variation of enzyme activity rate:
In formula, Δ RUraseFor urease activity dynamic change rate；
ΔRInvertaseFor invertase activity dynamic change rate；
RUrea nFor this measuring point urease activity；
RUrea n-1For upper measuring point urease activity；
RUrea 0Urease activity when for pollutant exposure concentrations being zero；
RConversion nFor this measuring point invertase activity；
RConversion n-1For upper measuring point invertase activity；
RConversion 0Invertase activity when for pollutant exposure concentrations being zero；
S3. chemical index TOC degradation rate is calculated
TOC degradation rate:
In formula: Δ RTOCFor the degradation rate of TOC；
For this measuring point TOC；
For upper measuring point TOC；
TOC when for pollutant exposure concentrations being zero.
S4. pass through Δ RUrase, Δ RInvertase, Δ RTOCThe case where characterizing sludge character Monitoring Indexes activated sludge.
Further, take multiple n obtained by leachate volume/absorption filtrate volume for described point.
The urase standard curve are as follows: y=4.8026x R2=0.999, wherein x is (aSample-aWithout sludge-aWithout matrix)；Institute
The invertase standard curve stated are as follows: y '=1.3335x ' R2=0.9991, wherein x ' is (a 'Sample-a’Without sludge-a’Without matrix)。
(aSample-aWithout sludge-aWithout matrix) or (a 'Sample-a’Without sludge-a’Without matrix) medicine, the aqueous solution that have removed in sludge, drug and aqueous mixtures can
Deviation caused by energy, therefore an entirety can be regarded as.
Urease activity of the present invention generates NH with 1g drying sludge afterwards for 24 hours3The milligram number of-N indicates；Invertase activity
With 1g drying sludge, rear glucogenic milligram number is indicated for 24 hours.
Enzyme is the protein or RNA generated by living cells, to its substrate with high degree of specificity and high catalytic efficiency,
It is a kind of important biocatalyst, has reaction condition mild, high catalytic efficiency, the advantages such as Substratspezifitaet is strong.EOCSIt is more
With different activities, the physiological and biochemical procedure of microorganism can be influenced.Common functional enzyme is undertaken in activated sludge sewage treatment
The mission of purification sewage, while its function also will receive in sewage and have the emerging EOCs of bioactivity is existing to influence.Research
The interaction mechanism and influence of EOCs and activated sludge functional enzyme obtain the interaction between them and influence system function
Risk in terms of data, can for comprehensively characterization activated sludge performance superiority and inferiority, assessment the steady and pre- bilge water resisting of activated sludge function at
Reason system risk lays the foundation.The present invention is with EOCs exposure concentrations and Biological indicators, the relationship of the dynamic change rate of chemical index
Indicate situation of change of each index under pollutant exposure.Since the degradation rate of total organic carbon (TOC) can characterize the place of sewage
Efficiency is managed, therefore selects TOC as chemical index.Urase is the hydrolase that itrogenous organic substance can be promoted to hydrolyze, can single-minded hydrolysis
Urea, while ammonia and carbon dioxide are released, it is of great significance to the decomposition of itrogenous organic substance in sewage.Invertase is can be with
The hydrolase for increasing ease of solubility nutriment in sludge, plays a significant role carbon cycle in sludge.Pass through TOC dynamic change rate
Compared with Biological indicators dynamic change rate, the sensibility of Biological indicators and chemical index is judged.
Beneficial effect
The present invention, can be fast to provide one by the sensibility of Biological indicators and chemical index in comparison activated sludge
Fast early warning Sludge Property changes or abnormal normal form, and this method assessment result accuracy is higher, can be steady to a certain extent
The efficiency for determining sewage treatment makes up the chemical index that sewage disposal system faces and monitors brought limitation, improves enterprise's wind
Dangerous prevention ability.
Detailed description of the invention
Fig. 1 urase standard curve；
Fig. 2 invertase standard curve；
The response of Fig. 3 Biological indicators, chemical index under bisphenol-A effect；Wherein, a is bisphenol-A to invertase activity
It influences；B is influence of the bisphenol-A to urease activity；C is influence of the bisphenol-A to TOC；
The response of Fig. 4 Biological indicators, chemical index under triclosan effect；Wherein, a is triclosan to invertase activity
Influence；B is influence of the triclosan to urease activity；C is influence of the triclosan to TOC.
Specific embodiment
The present invention is described in detail below by specific embodiment, but is not limited the scope of the invention.Unless otherwise specified, originally
Experimental method used by inventing is conventional method, and experiment equipment used, material, reagent etc. can chemically company be bought.
Activated sludge of the present invention is derived from Dalian sewage treatment plant aerobic tank, by pH be 6.9 activated sludge at 37 DEG C
Measure its enzymatic activity within constant temperature incubation 24 hours in incubator.Enzymatic activity when the pollutant exposure duration of various concentration gradient is 10h
More obvious with the dynamic change rate of TOC, following embodiments are by taking exposure duration is 10h as an example.
Influence of 1. bisphenol-A of embodiment to Biological indicators in activated sludge and chemical index
Influence of the 1. various concentration bisphenol-A of table to sludge organism index, chemical index dynamic change rate
Calculate step: for when bisphenol A concentration is 4 μ g/L, other situation calculating process are consistent with this,
(1)
(2)(aSample-aWithout sludge-aWithout matrix)=absorbance A/4.8026=0.0014/4.8026=0.00029
Urease activity=(aSample-aWithout sludge-aWithout matrix) nV/m=0.00029 × 2.5934 × 50/0.0015=25.07
(3)(aSample-aWithout sludge-aWithout matrix)=absorbance A/1.3335=0.8085/1.3335=0.6063
Invertase activity=(aSample-aWithout sludge-aWithout matrix) n/m=0.6063 × 2.5/0.0015=1010.56
Table 1 be shown in bisphenol A concentration be 4 μ g/L when, have certain inhibiting effect, Δ R to TOCInvertaseIt is Δ RTOC2.28
Times, Δ RUraseIt is Δ RTOC1.12 times；When bisphenol A concentration is 5 μ g/L, Δ RInvertaseIt is Δ RTOC13.15 times, Δ RUraseIt is Δ
RTOC20.72 times.Enzyme activities under the exposure of various concentration bisphenol-A are better than the variation of TOC, with the increase urea of bisphenol A concentration
Enzyme and invertase activity all obviously increase, can be according to selection urase or conversion under conditions of temperature and pH all more stable
Any one of enzyme monitors the dynamic change rate of its enzymatic activity, and then the variation that quickly early warning Sludge Property may occur.
Invertase activity and urease activity increase with the increase of bisphenol A concentration in Fig. 3, TOC with bisphenol A concentration increasing
Add first to reduce and increase afterwards, and amplitude of variation is smaller, is not easy to observe.Therefore, Biological indicators under the action of bisphenol-A are further illustrated in
Better than chemical index.
Influence of 2. triclosan of embodiment to Biological indicators in activated sludge and chemical index
Influence of the 2. various concentration triclosan of table to sludge organism index, chemical index dynamic change rate
Calculate step: by taking triclosan concentration is 0.5 μ g/L as an example, other situation calculating process are consistent with this,
(1)
(2)(aSample-aWithout sludge-aWithout matrix)=absorbance A/4.8026=0.0014/4.8026=0.00029
Urease activity=(aSample-aWithout sludge-aWithout matrix) nV/m=0.00029 × 2.4963 × 50/0.0015=24.131
(3)(aSample-aWithout sludge-aWithout matrix)=absorbance A/1.3335=0.9274/1.3335=0.6955
Invertase activity=(aSample-aWithout sludge-aWithout matrix) n/m=0.6955 × 3.5999/0.0015=1669.15
Table 2 be shown in triclosan concentration be 0.4 μ g/L when, have certain inhibiting effect, Δ R to TOCUraseIt is Δ RTOC's
33.67 times, Δ RInvertaseIt is Δ RTOC1.12 times；When triclosan concentration is 0.5 μ g/L, Δ RUraseIt is Δ RTOC17.14 times, Δ
RInvertaseIt is RTOC21.04 times.TOC variation under the exposure of various concentration triclosan is smaller, and the sensibility of Biological indicators is obvious
Better than chemical index, with the raising of triclosan concentration, urease activity decline destroys the ability of its oxidative metabolism；Invertase
Activity rises, and high density pollution object have stimulated its metabolic activity.It, can be according to three in the case where temperature and pH all more stable
The different selection urases or invertase of the raw concentration of chlorine monitor the dynamic change rate of its enzymatic activity, and then quickly early warning Sludge Property can
The variation that can occur.
Invertase activity first remains unchanged with the increase of triclosan concentration and increases afterwards in Fig. 4；Urease activity first increases and subtracts afterwards
It is small；TOC first reduces and increases afterwards, and variation range is basic it is considered that not changing less than 1.Therefore, it is further illustrated in trichlorine
Biological indicators are better than chemical index under the action of life.
Biological indicators urase and invertase are all more sensitive, but since different pollutant types and mechanism of action are different, no
Also different with Biological indicators sensibility, urase can promote the hydrolysis of itrogenous organic substance, certain dense when existing in aerobic activated sludge
When the noxious material of degree, the activity decline of the hydrolases such as urase may cause；The raising of invertase activity mentions for denitrifying bacteria
For sufficient carbon source, biological denitrificaion is carried out to supply nitrate reductase.Therefore, corresponding functional enzyme response has certain difference,
Generally speaking Biological indicators are better than chemical index.We can be realized by the monitoring to enzymatic activity to active sludge processing system
The prediction of middle Sludge Property abnormal conditions forms a kind of model of application function enzyme response early warning sewage disposal system early stage risk
Formula.
The preferable specific embodiment of the above, only the invention, but the protection scope of the invention is not
It is confined to this, anyone skilled in the art is in the technical scope that the invention discloses, according to the present invention
The technical solution of creation and its inventive concept are subject to equivalent substitution or change, should all cover the invention protection scope it
It is interior.
Claims (4)
1. a kind of normal form using sludge function enzyme response early warning sewage disposal system early stage risk, which is characterized in that
S1. sample constant temperature incubation 24 hours in 37 DEG C of incubator are taken, aerobic sludge function enzymatic activity is analyzed, according to
Formula (1) and formula (2) calculate urease activity and invertase activity；
Urease activity=(aSample-aWithout sludge-aWithout matrix)·nV/m (1)
Invertase activity=(a 'Sample-a’Without sludge-a’Without matrix)·n/m (2)
In formula, aSample, a 'SampleThe glucose in milligrams number or NH of standard curve calculating are corresponded to according to absorbance value for sample3- N milligrams
Number；
aWithout sludge, a 'Without sludgeThe glucose in milligrams number or NH of standard curve calculating are corresponded to according to absorbance value for no sludge3- N milligrams of numbers；
aWithout matrix, a 'Without matrixThe glucose in milligrams number or NH of standard curve calculating are corresponded to according to absorbance value for no matrix3- N milligrams of numbers；
N is to divide to take multiple；
M is drying sludge quality/g；
V is developing solution volume/mL；
S2. corresponding dynamic variation of enzyme activity rate is calculated separately by the resulting urease activity of S1, invertase activity；
Dynamic variation of enzyme activity rate:
In formula, Δ RUraseFor urease activity dynamic change rate；
ΔRInvertaseFor invertase activity dynamic change rate；
RUrea nFor this measuring point urease activity；
RUrea n-1For upper measuring point urease activity；
RUrea 0Urease activity when for pollutant exposure concentrations being zero；
RConversion nFor this measuring point invertase activity；
RConversion n-1For upper measuring point invertase activity；
RConversion 0Invertase activity when for pollutant exposure concentrations being zero；
S3. chemical index TOC degradation rate is calculated
TOC degradation rate:
In formula: Δ RTOCFor the degradation rate of TOC；
RTOC nFor this measuring point TOC；
RTOC n-1For upper measuring point TOC；
RTOC 0TOC when for pollutant exposure concentrations being zero；
S4. pass through Δ RUrase, Δ RInvertase, Δ RTOCThe case where characterizing sludge character Monitoring Indexes activated sludge.
2. the normal form according to claim 1 using sludge function enzyme response early warning sewage disposal system early stage risk,
It is characterized in that, described point takes multiple n obtained by leachate volume/absorption filtrate volume.
3. the normal form according to claim 1 using sludge function enzyme response early warning sewage disposal system early stage risk,
It is characterized in that, the urase standard curve are as follows: y=4.8026x R2=0.999, wherein x is (aSample-aWithout sludge-aWithout matrix)。
4. the normal form according to claim 1 using sludge function enzyme response early warning sewage disposal system early stage risk,
It is characterized in that, the invertase standard curve are as follows: y '=1.3335x ' R2=0.9991, wherein x ' is (a 'Sample-a’Without sludge-
a’Without matrix)。
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103645152A (en) * | 2013-12-12 | 2014-03-19 | 尹军 | Method for detecting activity of catalase in active sludge |
| CN106242154A (en) * | 2015-06-04 | 2016-12-21 | 三菱丽阳株式会社 | The method and device that waste water processes |
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2018
- 2018-12-13 CN CN201811526251.8A patent/CN109411026A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103645152A (en) * | 2013-12-12 | 2014-03-19 | 尹军 | Method for detecting activity of catalase in active sludge |
| CN106242154A (en) * | 2015-06-04 | 2016-12-21 | 三菱丽阳株式会社 | The method and device that waste water processes |
Non-Patent Citations (3)
| Title |
|---|
| 中国预防医学科学院环境卫生监测所；重庆市卫生防疫站: "《全球水质监测操作指南 联合国环境规划署 世界卫生组织 联合国教科文组织 世界气象组织 1987》", 31 December 1987, 中国预防医学科学院环境卫生监测所；重庆市卫生防疫站 * |
| 汪灵伟等: "双酚A对好氧活性污泥中不同酶活性的影响", 《武汉大学学报(理学版)》 * |
| 赵晶晶等: "布洛芬对活性污泥中功能酶活性的影响", 《生态毒理学报》 * |
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Application publication date: 20190301 |