CN109401967B - Lung cell culture device - Google Patents
Lung cell culture device Download PDFInfo
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- CN109401967B CN109401967B CN201811399403.2A CN201811399403A CN109401967B CN 109401967 B CN109401967 B CN 109401967B CN 201811399403 A CN201811399403 A CN 201811399403A CN 109401967 B CN109401967 B CN 109401967B
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- 238000004113 cell culture Methods 0.000 title claims abstract description 29
- 210000005265 lung cell Anatomy 0.000 title claims abstract description 28
- 238000000926 separation method Methods 0.000 claims abstract description 7
- 230000006835 compression Effects 0.000 claims description 32
- 238000007906 compression Methods 0.000 claims description 32
- 238000007789 sealing Methods 0.000 claims description 14
- 230000000903 blocking effect Effects 0.000 claims description 9
- 230000005540 biological transmission Effects 0.000 claims description 3
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims description 2
- 235000017491 Bambusa tulda Nutrition 0.000 claims description 2
- 241001330002 Bambuseae Species 0.000 claims description 2
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims description 2
- 239000011425 bamboo Substances 0.000 claims description 2
- 239000010408 film Substances 0.000 claims 8
- 230000002685 pulmonary effect Effects 0.000 claims 2
- 239000010409 thin film Substances 0.000 claims 1
- 239000012528 membrane Substances 0.000 description 13
- 210000004072 lung Anatomy 0.000 description 9
- 238000010586 diagram Methods 0.000 description 4
- 238000003780 insertion Methods 0.000 description 3
- 230000037431 insertion Effects 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 210000003205 muscle Anatomy 0.000 description 3
- 230000009471 action Effects 0.000 description 2
- 230000004888 barrier function Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 238000007792 addition Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
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Abstract
The invention relates to the relevant field of cell culture, in particular to a lung cell culture device which comprises an underframe, an ejection frame, a culture cylinder, a film baffle, a top cover and a front cover. The ejection rack is connected to the lower end of the bottom frame in a sliding mode, the culture cylinder is fixedly connected to the upper end of the bottom frame, the left end and the right end of the culture cylinder are fixedly connected with the film separation blades, the upper end of the culture cylinder is fixedly connected with the top cover, and the front end of the culture cylinder is fixedly connected with the front cover.
Description
Technical Field
The invention relates to the field related to cell culture, in particular to a lung cell culture device.
Background
The utility model discloses a cell culture device which can monitor the liquid environment of cell culture, and the application number is CN201320697500.6, the cell culture device comprises a monitor, a cell culture bottle, a sensor electrode and a sealing ring; the cell culture bottle is cuboid; one end of the cell culture bottle is provided with a bottle neck in an upward inclined way; at least two electrode sockets are arranged on the cell culture bottle; the electrode socket is in a cylindrical hole shape extending from the bottle body to the interior of the bottle cavity; the sensor electrode is inserted into the electrode socket through a sealing ring sleeved on the sensor electrode; the monitor is connected with the sensor electrode through a lead. The utility model has the advantages that: the utility model discloses a cell culture device that provides can monitor cell or the real-time concentration of certain specific material in the cell culture liquid among the tissue culture process, can study the influence of specific culture liquid environment to cell or tissue more accurately, dynamically. But the utility model can not simulate the dynamic environment of the lung and can culture the lung cells.
Disclosure of Invention
The invention provides a lung cell culture device which has the advantages that culture solution can be placed in the rectangular cylinder body to culture lung cells, two pressure columns can intermittently press two rubber membranes, the two rubber membranes simulate the movement of diaphragm muscles of the lung, the pressure in the rectangular cylinder body can be intermittently increased to simulate the dynamic environment of the lung, and therefore the lung cells are cultured in the dynamic environment, and the lung cells can be more truly cultured, researched and researched in an annular mode.
The invention relates to the relevant field of cell culture, in particular to a lung cell culture device which comprises an underframe, an ejection frame, a culture cylinder, a film baffle, a top cover and a front cover.
The ejection rack is connected to the lower end of the bottom frame in a sliding mode, the culture cylinder is fixedly connected to the upper end of the bottom frame, the left end and the right end of the culture cylinder are fixedly connected with the film separation blades, the upper end of the culture cylinder is fixedly connected with the top cover, and the front end of the culture cylinder is fixedly connected with the front cover.
The bottom frame comprises a supporting plate, vertical plates, compression legs, groove rods, convex seats I, fixed cylinders I, groove rod grooves, convex seats II, fixed cylinders II, fixed sleeves, trapezoidal slide rails, a motor frame plate, a motor, an incomplete gear and a limiting cylinder, wherein the vertical plates are fixedly connected at the left end and the right end of the supporting plate, the compression legs are respectively and slidably connected at the upper ends of the two vertical plates, the two convex seats I are respectively and fixedly connected at the middle ends of the outer sides of the two vertical plates, the fixed cylinders I are respectively and fixedly connected between the two convex seats I at the left end and the two convex seats I at the right end, the upper ends of the two groove rods are respectively and hingedly connected at the outer ends of the two compression legs, the groove rod grooves are respectively arranged on the two groove rods, the two fixed cylinders I are respectively and slidably connected at the middle ends of the two groove rod grooves, the fixed sleeves are respectively and fixedly connected at the left end and the right end of the lower end of the supporting plate, the convex seats II are respectively and transversely and slidably connected on the two fixed sleeves, the fixed cylinders II are respectively, two stationary cylinder II difference sliding connection are at the lower extreme in two groove pole grooves, the lower extreme left side fixedly connected with motor frame plate of layer board, the lower extreme fixedly connected with motor of motor frame plate, the incomplete gear of fixedly connected with on the right-hand member output shaft of motor, the tooth that is provided with the semicircle on the incomplete gear, the trapezoidal slide rail of lower extreme intermediate position fixedly connected with of layer board, the front end fixedly connected with of trapezoidal slide rail is spacing cylinder.
The ejection rack comprises a rack seat plate, a sliding ring, a rear baffle plate, an inclined rack rod, a compression spring, a spring sleeve rod, a sliding block, a front baffle plate, a constant arm rod and a rack, wherein the sliding block is connected on the trapezoidal sliding rail in a sliding manner, the sliding block is positioned at the rear end of the limiting cylinder, the left end and the right end of the lower end of the sliding block are respectively hinged with the constant arm rod, the other ends of the two constant arm rods are respectively hinged with the inner ends of the two convex seats II, the sliding block is fixedly connected with the front baffle plate, the rear end of the lower end of the front baffle plate is fixedly connected with the spring sleeve rod and the rack seat plate, the rack seat plate is positioned at the lower end of the spring sleeve rod, the spring sleeve rod is sleeved with the compression spring, the rear end of the spring sleeve rod is connected with the sliding ring, the compression spring is positioned between the sliding ring and the front baffle plate, the rear end of the spring sleeve rod is fixedly connected with the rear baffle plate, the sliding ring is positioned between the rear baffle plate and the front baffle plate, the upper end of the sliding ring is fixedly connected with two oblique frame rods, the upper ends of the two oblique frame rods are fixedly connected to the rear end of the supporting plate, the lower end of the rack seat plate is fixedly connected with a rack, and an incomplete gear is meshed with the rack for transmission.
The cultivation section of thick bamboo includes rectangular barrel, the nut, short double-screw bolt, rectangle side opening and rectangular channel, rectangular barrel fixed connection is in the upper end of layer board, both ends all are provided with the rectangle side opening about the rectangular barrel, the outside of two rectangle side openings all is provided with the rectangular channel, the equal short double-screw bolt of fixedly connected with in both ends around the rectangular barrel upper end, the upper end of two short double-screw bolts all has the nut through threaded connection, the upper end border position of rectangular barrel is provided with sealing washer I, both ends are inserted respectively on two short double-screw bolts around the top cap, the top cap compresses tightly on sealing washer I, two nuts all compress tightly the upper end at the top cap.
The film separation blade includes that the rectangle presses frame and rubber membrane, and the film separation blade is provided with two, and two rubber membranes bond respectively and seal two rectangle side openings in two rectangular channels departments, and the rectangle is pressed the frame all to have inserted in the outer end of two rectangular channels, and two rectangles press the frame to compress tightly respectively and fix two rubber membranes on two rubber membranes, and two rectangles press the equal fixed connection of frame on long barrel, and the inner of two compression columns is directional two rubber membranes respectively.
The front cover comprises a front cover plate, a discharge pipe and a valve, the discharge pipe is arranged at the lower end of the front cover plate, and the valve is arranged on the discharge pipe.
The chassis further comprises support posts, and the four corners of the lower end of the supporting plate are fixedly connected with the support posts.
The film separation blade further comprises screw inserting plates, the front end and the rear end of the rectangular pressing frame are fixedly connected with the screw inserting plates, and the two screw inserting plates are fixedly connected to the rectangular pressing frame through screws.
The culture cylinder further comprises a rectangular front hole, the front end of the rectangular cylinder body is provided with the rectangular front hole, and a sealing ring II is bonded at the edge position of the rectangular front hole.
The front cover plate is tightly pressed at the front end of the sealing ring II, the front cover plate is located at the position of the rectangular front hole, and the front cover plate is fixedly connected with the rectangular barrel through a screw.
The lung cell culture device has the beneficial effects that:
according to the lung cell culture device, culture solution can be placed in the rectangular cylinder body to culture lung cells, the two pressing columns can intermittently press the two rubber membranes, the two rubber membranes simulate the movement of diaphragm muscles of the lung, the pressure in the rectangular cylinder body can be intermittently increased, the dynamic environment of the lung is simulated, the lung cells are cultured in the dynamic environment, and the lung cells can be more truly cultured, researched and researched in an annular mode.
Drawings
The invention is described in further detail below with reference to the accompanying drawings and specific embodiments.
FIG. 1 is a schematic diagram showing the overall structure of a lung cell culture device according to the present invention;
FIG. 2 is a schematic diagram of the overall structure of a lung cell culture device according to the present invention;
FIG. 3 is a first schematic structural diagram of a chassis;
FIG. 4 is a second schematic structural view of the chassis;
FIG. 5 is a schematic view of a portion of the structure of the chassis;
fig. 6 is a first structural schematic diagram of the ejection rack;
FIG. 7 is a second schematic structural view of the ejector rack;
FIG. 8 is a first schematic structural view of a culture drum;
FIG. 9 is a second schematic structural view of the culture drum;
FIG. 10 is a first schematic structural view of a film barrier;
FIG. 11 is a second schematic structural view of the film barrier;
FIG. 12 is a schematic structural view of the top cover;
fig. 13 is a schematic structural view of the front cover.
In the figure: a chassis 1; 1-1 of a supporting plate; a vertical plate 1-2; 1-3 of a compression column; 1-4 of a grooved rod; a boss I1-5; a fixed cylinder I1-6; 1-7 of a slotted rod groove; a boss II 1-8; a fixed cylinder II 1-9; 1-10 parts of a fixing sleeve; 1-11 of a strut rod; 1-12 parts of a trapezoidal slide rail; 1-13 of motor frame plate; motors 1-14; 1-15 of incomplete gear; 1-16 parts of a limiting cylinder; an ejection rack 2; a rack seat plate 2-1; 2-2 of a smooth circular ring; 2-3 of a rear baffle plate; 2-4 of an oblique frame rod; 2-5 of a compression spring; 2-6 parts of a spring sleeve rod; 2-7 of a slide block; 2-8 parts of a front baffle plate; 2-9 of equal arm rods; 2-10 of racks; a culture cylinder 3; 3-1 of a rectangular cylinder; 3-2 parts of a nut; 3-3 of short studs; 3-4 rectangular side holes; 3-5 of rectangular groove; 3-6 rectangular front holes; a film baffle 4; a rectangular pressing frame 4-1; 4-2 parts of a rubber film; 4-3 of screw inserting plates; a top cover 5; a front cover 6; a front cover plate 6-1; 6-2 of a discharge pipe; and 6-3 of a valve.
Detailed Description
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on those shown in the drawings, and are used only for convenience in describing the present invention and for simplicity in description, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention. Furthermore, the terms "first", "second", etc. are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first," "second," etc. may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless otherwise specified.
The first embodiment is as follows:
the present invention relates to the field of cell culture, and more particularly to a lung cell culture device, which is described below with reference to fig. 1 to 13, and includes a bottom frame 1, an ejection frame 2, a culture cylinder 3, a film blocking sheet 4, a top cover 5 and a front cover 6, wherein a culture solution can be placed inside a rectangular cylinder 3-1 to culture lung cells, two compression columns 1-3 can intermittently compress two rubber films 4-2, the two rubber films 4-2 simulate the movement of the diaphragm muscle of the lung, and the pressure inside the rectangular cylinder 3-1 can intermittently increase to simulate the dynamic environment of the lung, so that the lung cells can be cultured in the dynamic environment, and the lung cells can be more truly cultured and circularly and researched.
The ejection rack 2 is slidably connected to the lower end of the bottom frame 1, the culture cylinder 3 is fixedly connected to the upper end of the bottom frame 1, the film blocking pieces 4 are fixedly connected to the left end and the right end of the culture cylinder 3, the top cover 5 is fixedly connected to the upper end of the culture cylinder 3, and the front cover 6 is fixedly connected to the front end of the culture cylinder 3.
The second embodiment is as follows:
the present embodiment is described below with reference to fig. 1 to 13, and the present embodiment further describes the first embodiment, where the base frame 1 includes a supporting plate 1-1, vertical plates 1-2, compression columns 1-3, groove rods 1-4, boss I1-5, fixed cylinders I1-6, groove rod grooves 1-7, boss II1-8, fixed cylinders II1-9, fixed sleeves 1-10, trapezoidal sliding rails 1-12, motor frame plates 1-13, motors 1-14, incomplete gears 1-15, and limit cylinders 1-16, the vertical plates 1-2 are fixedly connected to left and right ends of the supporting plate 1-1, the compression columns 1-3 are slidably connected to upper ends of the two vertical plates 1-2, and the two boss I1-5 are fixedly connected to middle ends of outer sides of the two vertical plates 1-2, fixed cylinders I1-6 are fixedly connected between two convex seats I1-5 positioned at the left end and between two convex seats I1-5 positioned at the right end, the upper ends of two groove rods 1-4 are respectively hinged at the outer ends of two compression columns 1-3, groove rod grooves 1-7 are respectively arranged on the two groove rods 1-4, two fixed cylinders I1-6 are respectively and slidably connected at the middle ends of the two groove rod grooves 1-7, the left and right ends of the lower end of the supporting plate 1-1 are respectively and fixedly connected with fixed sleeves 1-10, convex seats II1-8 are respectively and transversely and slidably connected on the two fixed sleeves 1-10, the outer ends of the two convex seats II1-8 are respectively and fixedly connected with fixed cylinders II1-9, the two fixed cylinders II1-9 are respectively and slidably connected at the lower ends of the two groove rod grooves 1-7, the left side of the lower end of the supporting plate 1-1 is fixedly connected with motor frame plates 1-13, the lower end of the motor frame plate 1-13 is fixedly connected with a motor 1-14, an output shaft at the right end of the motor 1-14 is fixedly connected with an incomplete gear 1-15, the incomplete gear 1-1 is provided with a half-circle of teeth, the middle position of the lower end of the supporting plate 1-1 is fixedly connected with a trapezoidal sliding rail 1-12, and the front end of the trapezoidal sliding rail 1-12 is fixedly connected with a limiting cylinder 1-16. The two convex seats II1-8 can respectively slide left and right on the two fixed sleeves 1-10, when the two convex seats II1-8 simultaneously slide outwards, the two fixed cylinders II1-9 simultaneously move outwards, so that the two fixed cylinders II1-9 respectively drive the lower ends of the two groove rods 1-4 to slide outwards, and as the two fixed cylinders I1-6 are respectively connected to the middle ends of the two groove rod grooves 1-7 in a sliding manner, the two compression columns 1-3 respectively approach inwards to slide at the upper ends of the two vertical plates 1-2; conversely, when the two bosses II1-8 slide inwards at the same time, the two compression legs 1-3 slide outwards and away from the upper ends of the two vertical plates 1-2 respectively. The output shafts of the motors 1 to 14 can drive the incomplete gears 1 to 15 to rotate when rotating.
The third concrete implementation mode:
the second embodiment is further described with reference to fig. 1-13, wherein the ejection rack 2 includes a rack seat plate 2-1, a sliding ring 2-2, a rear baffle plate 2-3, an inclined frame rod 2-4, a compression spring 2-5, a spring sleeve rod 2-6, a slider 2-7, a front baffle plate 2-8, an equal arm rod 2-9 and a rack 2-10, the slider 2-7 is slidably connected to a trapezoidal sliding rail 1-12, the slider 2-7 is located at the rear end of a limiting cylinder 1-16, the left and right ends of the lower end of the slider 2-7 are respectively and hingedly connected with the equal arm rods 2-9, the other ends of the two equal arm rods 2-9 are respectively and hingedly connected to the inner ends of two boss seats II1-8, the slider 2-7 is fixedly connected with the front baffle plate 2-8, the rear end of the lower end of the front baffle 2-8 is fixedly connected with a spring loop bar 2-6 and a rack seat plate 2-1, the rack seat plate 2-1 is positioned at the lower end of the spring loop bar 2-6, the spring loop bar 2-6 is sleeved with a compression spring 2-5, the rear end of the spring loop bar 2-6 is connected with a sliding circular ring 2-2 in a sliding way, the compression spring 2-5 is positioned between the sliding circular ring 2-2 and the front baffle 2-8, the rear end of the spring loop bar 2-6 is fixedly connected with a rear baffle 2-3, the sliding circular ring 2-2 is positioned between the rear baffle 2-3 and the front baffle 2-8, the lower end of the rear baffle 2-3 is fixedly connected at the rear end of the rack seat plate 2-1, the upper end of the sliding circular ring 2-2 is fixedly connected with two inclined frame bars 2-4, the upper ends of the two inclined frame bars 2-4 are both fixedly connected at the rear end of the supporting plate 1-1, the lower end of the rack seat plate 2-1 is fixedly connected with a rack 2-10, and the incomplete gear 1-15 is in meshing transmission with the rack 2-10. The sliding block 2-7 can slide back and forth on the trapezoidal sliding rail 1-12, the limiting cylinder 1-16 plays a limiting role, so that when the sliding block 2-7 is in contact with the limiting cylinder 1-16, the sliding block 2-7 cannot continuously slide forward, and when the incomplete gear 1-15 rotates, teeth on the incomplete gear 1-15 are meshed with the rack 2-10, so that the rack seat plate 2-1 and the spring sleeve rod 2-6 are driven to slide backwards, the sliding ring 2-2 is fixed, the spring sleeve rod 2-6 is compressed when sliding backwards, and when teeth on the incomplete gear 1-15 are separated from the rack 2-10, the sliding block 2-7 is quickly rebounded under the action of the compression spring 2-5; when the sliding block 2-7 slides backwards, the two equal arm rods 2-9 respectively drive the two convex seats II1-8 to slide outwards at the same time, and when the sliding block 2-7 slides forwards, the two convex seats II1-8 slide inwards at the same time.
The fourth concrete implementation mode:
the third embodiment is further described with reference to fig. 1-13, wherein the culture cylinder 3 comprises a rectangular cylinder 3-1, a nut 3-2, short studs 3-3, rectangular side holes 3-4 and rectangular grooves 3-5, the rectangular cylinder 3-1 is fixedly connected to the upper end of the supporting plate 1-1, the rectangular side holes 3-4 are respectively arranged at the left and right ends of the rectangular cylinder 3-1, the rectangular grooves 3-5 are respectively arranged at the outer sides of the two rectangular side holes 3-4, the short studs 3-3 are respectively fixedly connected to the front and rear ends of the upper end of the rectangular cylinder 3-1, the nut 3-2 is connected to the upper end of the two short studs 3-3 through threads, a sealing ring I is arranged at the edge position of the upper end of the rectangular cylinder 3-1, the front and rear ends of the top cover 5 are respectively inserted on the two short studs 3-3, the top cover 5 is tightly pressed on the sealing ring I, and the two nuts 3-2 are tightly pressed on the upper end of the top cover 5. The rectangular cylinder 3-1 can be filled with culture solution and lung cells to culture the lung cells. When the two nuts 3-2 are removed, the top cover 5 can be removed, and the cell culture solution can be placed into the rectangular cylinder 3-1.
The fifth concrete implementation mode:
the following describes the present embodiment with reference to fig. 1 to 13, which further describes the fourth embodiment, the film blocking piece 4 includes two rectangular pressing frames 4-1 and two rubber films 4-2, the film blocking piece 4 is provided with two rubber films 4-2 which are respectively bonded at two rectangular grooves 3-5 to seal two rectangular side holes 3-4, the rectangular pressing frames 4-1 are respectively inserted at the outer ends of the two rectangular grooves 3-5, the two rectangular pressing frames 4-1 are respectively pressed against the two rubber films 4-2 to fix the two rubber films 4-2, the two rectangular pressing frames 4-1 are both fixedly connected to the rectangular cylinder 3-1, and the inner ends of the two pressing columns 1-3 are respectively directed to the two rubber films 4-2. When the two compression columns 1-3 slide inwards at the same time, the rubber membrane 4-2 is compressed inwards to work by simulating the diaphragm of the lung, at the moment, the pressure in the rectangular cylinder 3-1 is increased, when the two compression columns 1-3 rebound outwards rapidly, the rubber membrane 4-2 is restored to the original state, and the pressure in the rectangular cylinder 3-1 is restored to the initial pressure.
The sixth specific implementation mode:
the fourth embodiment is further described with reference to fig. 1-13, wherein the front cover 6 includes a front cover plate 6-1, a discharge pipe 6-2 and a valve 6-3, the discharge pipe 6-2 is disposed at the lower end of the front cover plate 6-1, and the valve 6-3 is disposed on the discharge pipe 6-2. After the valve 6-3 is opened, the culture solution and the lung cells in the rectangular cylinder 3-1 can be discharged from the discharge pipe 6-2.
The seventh embodiment:
the present embodiment is described below with reference to fig. 1 to 13, and the second embodiment is further described in the present embodiment, where the base frame 1 further includes support rods 1 to 11, and the support rods 1 to 11 are fixedly connected to four corners of the lower end of the supporting plate 1 to 1. The four support posts 1-11 function to support the pallet 1-1.
The specific implementation mode is eight:
the embodiment is described below with reference to fig. 1 to 13, and the fifth embodiment is further described in the present embodiment, the film blocking sheet 4 further includes screw insertion plates 4-3, the front and rear ends of the rectangular pressing frame 4-1 are fixedly connected with the screw insertion plates 4-3, and the two screw insertion plates 4-3 are fixedly connected to the rectangular pressing frame 4-1 through screws. The screw inserting plate 4-3 is fixedly connected to the rectangular pressing frame 4-1 through screws, and the film blocking piece 4 can be integrally taken down by dismounting the screws on the screw inserting plate 4-3, so that the rubber film 4-2 can be replaced when the rubber film 4-2 is damaged.
The specific implementation method nine:
the sixth embodiment is further described with reference to fig. 1-13, wherein the culture cylinder 3 further comprises a rectangular front hole 3-6, the front end of the rectangular cylinder 3-1 is provided with the rectangular front hole 3-6, and a sealing ring II is bonded to the edge of the rectangular front hole 3-6.
The detailed implementation mode is ten:
the following describes the present embodiment with reference to fig. 1 to 13, and the present embodiment further describes an embodiment nine, where the front cover plate 6-1 is pressed against the front end of the sealing ring II, the front cover plate 6-1 is located at the position of the rectangular front hole 3-6, and the front cover plate 6-1 is fixedly connected with the rectangular cylinder 3-1 through a screw. The front cover plate 6-1 is fixedly connected to the rectangular front hole 3-6 through a screw, the front cover plate 6-1 can be detached by detaching the screw on the front cover plate 6-1, and the inside of the rectangular cylinder 3-1 is convenient to clean.
The working principle of the invention is as follows: the two convex seats II1-8 can respectively slide left and right on the two fixed sleeves 1-10, when the two convex seats II1-8 simultaneously slide outwards, the two fixed cylinders II1-9 simultaneously move outwards, so that the two fixed cylinders II1-9 respectively drive the lower ends of the two groove rods 1-4 to slide outwards, and as the two fixed cylinders I1-6 are respectively connected to the middle ends of the two groove rod grooves 1-7 in a sliding manner, the two compression columns 1-3 respectively approach inwards to slide at the upper ends of the two vertical plates 1-2; conversely, when the two bosses II1-8 slide inwards at the same time, the two compression legs 1-3 slide outwards and away from the upper ends of the two vertical plates 1-2 respectively. The output shafts of the motors 1 to 14 can drive the incomplete gears 1 to 15 to rotate when rotating. The sliding block 2-7 can slide back and forth on the trapezoidal sliding rail 1-12, the limiting cylinder 1-16 plays a limiting role, so that when the sliding block 2-7 is in contact with the limiting cylinder 1-16, the sliding block 2-7 cannot continuously slide forward, and when the incomplete gear 1-15 rotates, teeth on the incomplete gear 1-15 are meshed with the rack 2-10, so that the rack seat plate 2-1 and the spring sleeve rod 2-6 are driven to slide backwards, the sliding ring 2-2 is fixed, the spring sleeve rod 2-6 is compressed when sliding backwards, and when teeth on the incomplete gear 1-15 are separated from the rack 2-10, the sliding block 2-7 is quickly rebounded under the action of the compression spring 2-5; when the sliding block 2-7 slides backwards, the two equal arm rods 2-9 respectively drive the two convex seats II1-8 to slide outwards at the same time, and when the sliding block 2-7 slides forwards, the two convex seats II1-8 slide inwards at the same time. The rectangular cylinder 3-1 can be filled with culture solution and lung cells to culture the lung cells. When the two nuts 3-2 are removed, the top cover 5 can be removed, and the cell culture solution can be placed into the rectangular cylinder 3-1. When the two compression columns 1-3 slide inwards at the same time, the rubber membrane 4-2 is compressed inwards to work by simulating the diaphragm of the lung, at the moment, the pressure in the rectangular cylinder 3-1 is increased, when the two compression columns 1-3 rebound outwards rapidly, the rubber membrane 4-2 is restored to the original state, and the pressure in the rectangular cylinder 3-1 is restored to the initial pressure. After the valve 6-3 is opened, the culture solution and the lung cells in the rectangular cylinder 3-1 can be discharged from the discharge pipe 6-2. The four support posts 1-11 function to support the pallet 1-1. The screw inserting plate 4-3 is fixedly connected to the rectangular pressing frame 4-1 through screws, and the film blocking piece 4 can be integrally taken down by dismounting the screws on the screw inserting plate 4-3, so that the rubber film 4-2 can be replaced when the rubber film 4-2 is damaged. The front cover plate 6-1 is fixedly connected to the rectangular front hole 3-6 through a screw, the front cover plate 6-1 can be detached by detaching the screw on the front cover plate 6-1, and the inside of the rectangular cylinder 3-1 is convenient to clean.
It is to be understood that the above description is not intended to limit the present invention, and the present invention is not limited to the above examples, and that various changes, modifications, additions and substitutions which are within the spirit and scope of the present invention and which may be made by those skilled in the art are also within the scope of the present invention.
Claims (3)
1. The utility model provides a lung cell culture device, includes chassis (1), ejection rack (2), culture section of thick bamboo (3), film separation blade (4), top cap (5) and protecgulum (6), its characterized in that: the ejection frame (2) is connected to the lower end of the bottom frame (1) in a sliding mode, the culture cylinder (3) is fixedly connected to the upper end of the bottom frame (1), the left end and the right end of the culture cylinder (3) are both fixedly connected with the thin film blocking pieces (4), the upper end of the culture cylinder (3) is fixedly connected with the top cover (5), and the front end of the culture cylinder (3) is fixedly connected with the front cover (6);
the bottom frame (1) comprises a supporting plate (1-1), vertical plates (1-2), compression legs (1-3), groove rods (1-4), convex seats I (1-5), fixed cylinders I (1-6), groove rod grooves (1-7), convex seats II (1-8), fixed cylinders II (1-9), fixed sleeves (1-10), trapezoidal sliding rails (1-12), motor frame plates (1-13), motors (1-14), incomplete gears (1-15) and limiting cylinders (1-16), the vertical plates (1-2) are fixedly connected to the left end and the right end of the supporting plate (1-1), the compression legs (1-3) are slidably connected to the upper ends of the two vertical plates (1-2), and the two convex seats I (1-5) are fixedly connected to the middle ends of the outer sides of the two vertical plates (1-2), the fixing cylinders I (1-6) are fixedly connected between the two convex seats I (1-5) at the left end and between the two convex seats I (1-5) at the right end, the upper ends of the two groove rods (1-4) are respectively hinged to the outer ends of the two compression columns (1-3), the two groove rods (1-4) are respectively provided with a groove rod groove (1-7), the two fixing cylinders I (1-6) are respectively connected to the middle ends of the two groove rod grooves (1-7) in a sliding manner, the left end and the right end of the lower end of the supporting plate (1-1) are respectively and fixedly connected with a fixing sleeve (1-10), the two fixing sleeves (1-10) are respectively and transversely connected with convex seats II (1-8) in a sliding manner, the outer ends of the two convex seats II (1-8) are respectively and fixedly connected with a fixing cylinder II (1-9), two fixing cylinders II (1-9) are respectively connected to the lower ends of the two groove rod grooves (1-7) in a sliding manner, the left side of the lower end of the supporting plate (1-1) is fixedly connected with a motor frame plate (1-13), the lower end of the motor frame plate (1-13) is fixedly connected with a motor (1-14), an output shaft at the right end of the motor (1-14) is fixedly connected with an incomplete gear (1-15), the incomplete gear (1-15) is provided with a half-circle of teeth, the middle position of the lower end of the supporting plate (1-1) is fixedly connected with a trapezoidal sliding rail (1-12), and the front end of the trapezoidal sliding rail (1-12) is fixedly connected with a limiting cylinder (1-16);
the ejection rack (2) comprises a rack seat plate (2-1), a sliding ring (2-2), a rear baffle (2-3), an inclined rack rod (2-4), a compression spring (2-5), a spring loop rod (2-6), a sliding block (2-7), a front baffle (2-8), an equal arm rod (2-9) and a rack (2-10), wherein the sliding block (2-7) is connected to a trapezoidal sliding rail (1-12) in a sliding manner, the sliding block (2-7) is positioned at the rear end of a limiting cylinder (1-16), the left end and the right end of the lower end of the sliding block (2-7) are respectively hinged to the inner ends of two convex seats II (1-8), the sliding block (2-7) is fixedly connected with the front baffle (2-8), the rear end of the lower end of the front baffle (2-8) is fixedly connected with a spring loop bar (2-6) and a rack seat plate (2-1), the rack seat plate (2-1) is positioned at the lower end of the spring loop bar (2-6), the spring loop bar (2-6) is sleeved with a compression spring (2-5), the rear end of the spring loop bar (2-6) is connected with a sliding circular ring (2-2) in a sliding manner, the compression spring (2-5) is positioned between the sliding circular ring (2-2) and the front baffle (2-8), the rear end of the spring loop bar (2-6) is fixedly connected with a rear baffle (2-3), the sliding circular ring (2-2) is positioned between the rear baffle (2-3) and the front baffle (2-8), the lower end of the rear baffle (2-3) is fixedly connected at the rear end of the rack seat plate (2-1), the upper end of the sliding circular ring (2-2) is fixedly connected with two inclined frame rods (2-4), the upper ends of the two inclined frame rods (2-4) are fixedly connected to the rear end of the supporting plate (1-1), the lower end of the rack seat plate (2-1) is fixedly connected with a rack (2-10), and the incomplete gear (1-15) is in meshing transmission with the rack (2-10);
the culture cylinder (3) comprises a rectangular cylinder body (3-1), nuts (3-2), short studs (3-3), rectangular side holes (3-4) and rectangular grooves (3-5), the rectangular cylinder body (3-1) is fixedly connected to the upper end of the supporting plate (1-1), the rectangular side holes (3-4) are formed in the left end and the right end of the rectangular cylinder body (3-1), the rectangular grooves (3-5) are formed in the outer sides of the two rectangular side holes (3-4), the short studs (3-3) are fixedly connected to the front end and the rear end of the upper end of the rectangular cylinder body (3-1), the nuts (3-2) are connected to the upper ends of the two short studs (3-3) through threads, a sealing ring I is arranged at the edge position of the upper end of the rectangular cylinder body (3-1), and the front end and rear end of a top cover (5) are respectively inserted into the two short studs (3-3), the top cover (5) is tightly pressed on the sealing ring I, and the two nuts (3-2) are tightly pressed at the upper end of the top cover (5);
the film separation blade (4) comprises two rectangular pressing frames (4-1) and two rubber films (4-2), the two rubber films (4-2) are respectively bonded at the two rectangular grooves (3-5) to seal the two rectangular side holes (3-4), the rectangular pressing frames (4-1) are inserted at the outer ends of the two rectangular grooves (3-5), the two rectangular pressing frames (4-1) are respectively pressed on the two rubber films (4-2) to fix the two rubber films (4-2), the two rectangular pressing frames (4-1) are respectively fixedly connected to the rectangular cylinder body (3-1), and the inner ends of the two pressing columns (1-3) respectively point to the two rubber films (4-2);
the front cover (6) comprises a front cover plate (6-1), a discharge pipe (6-2) and a valve (6-3), the discharge pipe (6-2) is arranged at the lower end of the front cover plate (6-1), and the valve (6-3) is arranged on the discharge pipe (6-2);
the film blocking piece (4) further comprises screw inserting plates (4-3), the front end and the rear end of the rectangular pressing frame (4-1) are fixedly connected with the screw inserting plates (4-3), and the two screw inserting plates (4-3) are fixedly connected to the rectangular pressing frame (4-1) through screws;
the front cover plate (6-1) is tightly pressed at the front end of the sealing ring II, the front cover plate (6-1) is located at the position of the rectangular front hole (3-6), and the front cover plate (6-1) is fixedly connected with the rectangular cylinder (3-1) through a screw.
2. The pulmonary cell culture device of claim 1, wherein: the chassis (1) further comprises support posts (1-11), and the four corners of the lower end of the support plate (1-1) are fixedly connected with the support posts (1-11).
3. The pulmonary cell culture device of claim 2, wherein: the culture cylinder (3) further comprises a rectangular front hole (3-6), the rectangular front hole (3-6) is formed in the front end of the rectangular cylinder body (3-1), and a sealing ring II is bonded to the edge of the rectangular front hole (3-6).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811399403.2A CN109401967B (en) | 2018-11-22 | 2018-11-22 | Lung cell culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811399403.2A CN109401967B (en) | 2018-11-22 | 2018-11-22 | Lung cell culture device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109401967A CN109401967A (en) | 2019-03-01 |
| CN109401967B true CN109401967B (en) | 2021-07-23 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201811399403.2A Expired - Fee Related CN109401967B (en) | 2018-11-22 | 2018-11-22 | Lung cell culture device |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10030528A1 (en) * | 2000-06-28 | 2002-01-24 | Endlich Nicole | Apparatus for mechanical dilation of cultured cells, to simulate effect of respiration on lung cells, comprises membrane which acts as support for cells and to which vacuum or pressure can be applied via pump |
| CN105142652A (en) * | 2013-01-08 | 2015-12-09 | 耶鲁大学 | Human and large-mammal lung bioreactor |
| CN108705795A (en) * | 2018-05-19 | 2018-10-26 | 李冬年 | A kind of biological medicine research testing stand |
-
2018
- 2018-11-22 CN CN201811399403.2A patent/CN109401967B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10030528A1 (en) * | 2000-06-28 | 2002-01-24 | Endlich Nicole | Apparatus for mechanical dilation of cultured cells, to simulate effect of respiration on lung cells, comprises membrane which acts as support for cells and to which vacuum or pressure can be applied via pump |
| CN105142652A (en) * | 2013-01-08 | 2015-12-09 | 耶鲁大学 | Human and large-mammal lung bioreactor |
| CN108705795A (en) * | 2018-05-19 | 2018-10-26 | 李冬年 | A kind of biological medicine research testing stand |
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| CN109401967A (en) | 2019-03-01 |
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Inventor after: Ni Xiaofeng Inventor after: Liu Xia Inventor after: Shaping treasure Inventor before: Shaping treasure |
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Effective date of registration: 20210702 Address after: 111 Chunqiu West Road, Qufu City, Jining City, Shandong Province 273100 (Qufu people's Hospital) Applicant after: Ni Xiaofeng Address before: Academy of Sciences, Harbin Institute of technology, No.92 xidazhi street, Nangang District, Harbin City, Heilongjiang Province, 150006 Applicant before: Shaping treasure |
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