CN109401942A - A kind of sequencing optical system - Google Patents
A kind of sequencing optical system Download PDFInfo
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- CN109401942A CN109401942A CN201811558439.0A CN201811558439A CN109401942A CN 109401942 A CN109401942 A CN 109401942A CN 201811558439 A CN201811558439 A CN 201811558439A CN 109401942 A CN109401942 A CN 109401942A
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- 230000003287 optical effect Effects 0.000 title claims abstract description 84
- 238000012163 sequencing technique Methods 0.000 title claims abstract description 28
- 230000007246 mechanism Effects 0.000 claims abstract description 71
- 238000003384 imaging method Methods 0.000 claims abstract description 20
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 claims description 9
- 238000013016 damping Methods 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 2
- 108090000623 proteins and genes Proteins 0.000 abstract description 11
- 230000005540 biological transmission Effects 0.000 description 5
- 238000010586 diagram Methods 0.000 description 5
- 230000002452 interceptive effect Effects 0.000 description 4
- 230000001678 irradiating effect Effects 0.000 description 3
- 238000012423 maintenance Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 230000004313 glare Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 1
- 229910052753 mercury Inorganic materials 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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- Life Sciences & Earth Sciences (AREA)
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- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
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- Health & Medical Sciences (AREA)
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- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Microscoopes, Condenser (AREA)
Abstract
The invention discloses a kind of sequencing optical systems, it belongs to gene sequencing field, including optical filter switching mechanism, light-transmitting means, object lens and imaging mechanism, the first reflection subassembly is arranged in one end of light-transmitting means, the first hood is provided with outside first reflection subassembly, the side of light-transmitting means is provided with the first shading sleeve connecting with the first reflection subassembly, and the first shading sleeve is connect with optical filter switching mechanism；One end of object lens is connect by the second shading sleeve with optical filter switching mechanism；The second reflection subassembly and imager is respectively set in the both ends of imaging mechanism, the second hood is arranged outside the second reflection subassembly, the second hood and the second reflection subassembly are connect with optical filter switching mechanism.The light that light-transmitting means issues passes through the first reflection subassembly and injects optical filter switching mechanism, emits by optical filter towards object lens, transmitting fluorescence emits after optical filter towards imaging mechanism from object lens after sample is illuminated.
Description
Technical field
The present invention relates to gene sequencing field more particularly to a kind of sequencing optical systems.
Background technique
Gene sequencing can analyze measurement gene complete sequence from blood or saliva, and the possibility of a variety of diseases is suffered from prediction
Property.
The method that fluorescent marker is commonly used carries out gene sequencing, needs to be arranged optical system use in gene sequencer
In excitation fluorescent marker and acquire the fluorescence signal that these fluorescent markers are launched (fluorescence signal is light).Carry out
Before gene sequencing, need in optical system object lens and gene sequencing chip focus, when carrying out gene sequencing, need
Operation of continuously taking pictures is carried out to detection sample by picture pick-up device.
But structure is complicated and the accuracy of focusing is not high for gene sequencer focusing system in the prior art, meanwhile,
Existing gene sequencer optical system overall structure is also more complicated, therefore causes to debug very inconvenient, production maintenance cost
Height, accuracy be not high.It currently when take pictures operation, is taken pictures by the relative complex optical module of structure
Operation, although can satisfy using needs, operation and maintenance cost are relatively high, and regulating power is relatively low, Bu Nengyou
The needs of the satisfaction actual use of effect, while also causing the precision for detecting operation of taking pictures relatively low.
Summary of the invention
The purpose of the present invention is to provide a kind of sequencing optical system, with solve debugging existing in the prior art it is inconvenient,
The technical problem that production maintenance is at high cost, precision is low.
As above design, the technical scheme adopted by the invention is that:
A kind of sequencing optical system, comprising:
Optical filter switching mechanism；
Light-transmitting means, one end are provided with the first reflection subassembly, are provided with the first screening outside first reflection subassembly
Light shield, the side of the light-transmitting means are provided with the first shading sleeve connecting with first reflection subassembly, and described first
Shading sleeve is connect with the optical filter switching mechanism；
Object lens, one end are connect by the second shading sleeve with the optical filter switching mechanism, and the side of the object lens is set
It is equipped with automatic focusing mechanism；
Imaging mechanism, one end are provided with the second reflection subassembly, and the other end is provided with imager, second reflection subassembly
Outside is provided with the second hood, and second hood and second reflection subassembly connect with the optical filter switching mechanism
It connects.
Wherein, the light-transmitting means includes guide cylinder, and first reflection subassembly is set to one end of the guide cylinder,
The other end of the guide cylinder is provided with light source.
Wherein, the first shading sleeve includes that the first inner cylinder and sliding sleeve are set to outside first on first inner cylinder
Cylinder, first inner cylinder are fixedly connected with first hood, and first outer cylinder is connect with the optical filter switching mechanism.
Wherein, the optical filter switching mechanism includes cabinet, and light inlet is offered on the cabinet and two are oppositely arranged
Light-emitting window, the first shading sleeve is set at the light inlet, and the object lens and the imaging mechanism are respectively arranged at
At two light-emitting windows.
Wherein, it is provided with motor in the cabinet, is nibbled with the gear of the output axis connection of the motor and with the gear
The rack gear of conjunction, the moving direction on the rack gear along the rack gear are arranged at intervals with the first optical filter and the second optical filter.
Wherein, the second shading sleeve includes that the second inner cylinder and sliding sleeve are set to outside second on second inner cylinder
Cylinder, second inner cylinder are connect with the optical filter switching mechanism, and second outer cylinder is connect with the object lens.
Wherein, the automatic focusing mechanism includes power motor and the mobile branch that connect with the output end of the power motor
Seat, the object lens are connect with the mobile support saddle.
Wherein, the imaging mechanism includes transmitting cylinder, and second reflection subassembly and the imager are located at described
Transmit the both ends of cylinder.
Wherein, further include pedestal, the optical filter switching mechanism, the light-transmitting means, the automatic focusing mechanism and
The imaging mechanism is mounted on the pedestal.
Wherein, elastic damping component is provided between the automatic focusing mechanism and the pedestal.
Beneficial effects of the present invention:
Sequencing optical system proposed by the present invention, the light that light-transmitting means issues pass through the reflection of the first reflection subassembly, penetrate
Enter optical filter switching mechanism, reflected by optical filter, is emitted towards object lens, by the eyeglass irradiating sample of object lens, sample is shone
Emit fluorescence after penetrating from object lens directive optical filter switching mechanism, emits after passing through optical filter towards imaging mechanism, ultimately produce figure
Picture.Because the first reflection subassembly and the second reflection subassembly are arranged at corner, the first hood, the second hood, the first shading
The setting of sleeve and the second shading sleeve, is used to closed shading, prevents extraneous light from interfering light source, improve sequencing
Accuracy.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of sequencing optical system provided in an embodiment of the present invention；
Fig. 2 is the partial structure diagram of Fig. 1；
Fig. 3 is the structural schematic diagram of the optical filter switching mechanism of Fig. 1；
Fig. 4 is the partial structure diagram of Fig. 3；
Fig. 5 is the structural schematic diagram of the imaging mechanism of Fig. 1.
In figure:
1, pedestal；
2, optical filter switching mechanism；21, cabinet；211, light inlet；212, light-emitting window；22, motor；23, rack gear；24,
One optical filter；25, the second optical filter；
3, light-transmitting means；31, guide cylinder；32, the first reflection subassembly；33, light source；34, the first hood；35, first
Shading sleeve；351, the first inner cylinder；352, the first outer cylinder；
41, object lens；42, the second shading sleeve；
5, automatic focusing mechanism；51, power motor；52, mobile support saddle；
6, imaging mechanism；61, cylinder is transmitted；62, imager；63, the second hood.
Specific embodiment
To keep the technical problems solved, the adopted technical scheme and the technical effect achieved by the invention clearer, below
In conjunction with attached drawing and pass through specific embodiment to further illustrate the technical scheme of the present invention.It is understood that this place is retouched
The specific embodiment stated is used only for explaining the present invention rather than limiting the invention.It also should be noted that in order to just
Only the parts related to the present invention are shown in description, attached drawing and it is not all.
Referring to Fig. 1 to Fig. 5, the embodiment of the present invention provides a kind of sequencing optical system, including pedestal 1, is equipped on pedestal 1
Optical filter switching mechanism 2, light-transmitting means 3, automatic focusing mechanism 5 and imaging mechanism 6.
One end of light-transmitting means 3 is provided with the first reflection subassembly 32, is provided with the first screening outside the first reflection subassembly 32
Light shield 34, the side of light-transmitting means 3 are provided with the first shading sleeve 35 connecting with the first reflection subassembly 32, the first shading sleeve
Cylinder 35 is connect with optical filter switching mechanism 2；One end of object lens 41 is connected by the second shading sleeve 42 with optical filter switching mechanism 2
It connects, the side of object lens 41 is provided with automatic focusing mechanism 5；One end of imaging mechanism 6 is provided with the second reflection subassembly, and the other end is set
It is equipped with imager 62, is provided with the second hood 63 outside the second reflection subassembly, the second hood 63 and the second reflection subassembly are equal
It is connect with optical filter switching mechanism 2.
The light that light-transmitting means 3 issues passes through the reflection of the first reflection subassembly 32, injects optical filter switching mechanism 2, passes through
Optical filter reflection, emits towards object lens 41, and by the eyeglass irradiating sample of object lens 41, fluorescence is emitted after sample is illuminated from object lens
41 directive optical filter switching mechanisms 2 make the biography of light after optical filter by the second hood 63 and the second reflection subassembly
After defeated path changes 90 degree, emits towards imaging mechanism 6, ultimately produce image.Because of the first reflection subassembly 32 and the second reflection group
Part is arranged at corner, and the first hood 34, the second hood 63, the first shading sleeve 35 and the second shading sleeve 42 are set
It sets, is used to closed shading, prevent extraneous light from interfering light source 33, improve the accuracy of sequencing.
Light-transmitting means 3 includes guide cylinder 31, and the first reflection subassembly 32 is set to one end of guide cylinder 31, guide cylinder 31
The other end is provided with light source 33.In the present embodiment, adjusting window is offered on guide cylinder 31, for adjusting the degree of focus of light source 33.
The light that light-transmitting means 3 issues propagates at the first reflection subassembly 32 along guide cylinder 31, is reflected by the first reflection subassembly 32.First
Reflection subassembly 32 is 45 degree of reflective mirrors being obliquely installed, so that the transmission path of light changes 90 degree.In the present embodiment, light source
33 be mercury lamp or LED.
First shading sleeve 35 includes the first outer cylinder 352 of the first inner cylinder 351 and sliding sleeve on the first inner cylinder 351,
First inner cylinder 351 is fixedly connected with the first hood 34, and the first outer cylinder 352 is connect with optical filter switching mechanism 2.First shading sleeve
The structure setting of cylinder 35 convenient for adjusting the spacing of the first hood 34 and optical filter switching mechanism 2 according to actual needs, and guarantees
The airtightness of optical path prevents extraneous light from interfering light source 33.
Optical filter switching mechanism 2 includes cabinet 21, and light inlet 211 and two light out being oppositely arranged are offered on cabinet 21
Mouth 212, the first shading sleeve 35 is set at light inlet 211, and object lens 41 and imaging mechanism 6 are respectively arranged at two light-emitting windows
At 212.The light that light-transmitting means 3 issues passes through the first reflection subassembly 32, and transmission path changes 90 degree and injected by light inlet 211
Optical filter switching mechanism 2, reflects by optical filter, and transmission path changes 90 degree and passes through one of light-emitting window 212 towards object lens
41 transmittings, pass through the eyeglass irradiating sample of object lens 41.
Be provided in cabinet 21 motor 22, with the gear of the output axis connection of motor 22 and the rack gear 23 engaged with gear,
Moving direction on rack gear 23 along rack gear 23 is arranged at intervals with the first optical filter 24 and the second optical filter 25.Motor 22 passes through gear
Band carry-over bar 23 is mobile, and then changes the position of the first optical filter 24 and the second optical filter 25 so that the first optical filter 24 or
At second optical filter, 25 face light inlet 211.First optical filter 24 and the second optical filter 25 can be used in filtering different wave length
Light.
Second shading sleeve 42 includes the second outer cylinder that the second inner cylinder and sliding sleeve are set on the second inner cylinder, the second inner cylinder with
Optical filter switching mechanism 2 connects, and the second outer cylinder is connect with object lens 41.The structure setting of second shading sleeve 42, convenient for focusing
In the process, the airtightness for guaranteeing optical path prevents extraneous light from interfering light source 33.
Automatic focusing mechanism 5 includes power motor 51 and the mobile support saddle 52 connecting with the output end of power motor 51, object
Mirror 41 is connect with mobile support saddle 52.The output shaft of power motor 51 drives mobile support saddle 52 mobile by transmission mechanism, and then adjusts
Save the focal length of object lens 41.In order to guarantee the stability adjusted, elastic damping group is provided between automatic focusing mechanism 5 and pedestal 1
Part.
Imaging mechanism 6 includes transmitting cylinder 61, and the second reflection subassembly and imager 62 are located at the both ends of transmitting cylinder 61.Sample
Transmitting fluorescence is from 41 directive optical filter switching mechanism 2 of object lens after product are illuminated, from another 212 court of light-emitting window after optical filter
Emit to imaging mechanism 6, be irradiated to the second reflection subassembly, transmission path changes 90 and exposes to imager 62, ultimately produces image.
In the present embodiment, transmitting cylinder 61 is identical as the extending direction of guide cylinder 31, and is located at the same side of pedestal 61, so that structure is tight
It gathers, saves space hold.
In addition to above-mentioned first hood 34, the second hood 63, the first shading sleeve 35 and the second shading sleeve 42, light
Each interface of road part is all made of sealing means, and veiling glare is avoided to influence.
Embodiment of above only elaborates that the basic principles and features of the invention, the present invention are not limited by above embodiment
System, without departing from the spirit and scope of the present invention, the invention also has various changes and changes, and these changes and changes are all
It falls into scope of the claimed invention.The claimed scope of the invention is by appended claims and its equivalent circle
It is fixed.
Claims (10)
1. a kind of sequencing optical system characterized by comprising
Optical filter switching mechanism (2)；
Light-transmitting means (3), one end are provided with the first reflection subassembly (32), are provided with outside first reflection subassembly (32)
First hood (34), the side of the light-transmitting means (3) are provided with first connect with first reflection subassembly (32)
Shading sleeve (35), the first shading sleeve (35) connect with the optical filter switching mechanism (2)；
Object lens (41), one end are connect by the second shading sleeve (42) with the optical filter switching mechanism (2), the object lens
(41) side is provided with automatic focusing mechanism (5)；
Imaging mechanism (6), one end are provided with the second reflection subassembly, and the other end is provided with imager (62), second reflection
Be provided with the second hood (63) outside component, second hood (63) and second reflection subassembly with the optical filtering
Piece switching mechanism (2) connection.
2. sequencing optical system according to claim 1, which is characterized in that the light-transmitting means (3) includes guide cylinder
(31), first reflection subassembly (32) is set to one end of the guide cylinder (31), and the other end of the guide cylinder (31) is set
It is equipped with light source (33).
3. sequencing optical system according to claim 1, which is characterized in that the first shading sleeve (35) includes first
Inner cylinder (351) and sliding sleeve are set to the first outer cylinder (352) on first inner cylinder (351), first inner cylinder (351) and institute
It states the first hood (34) to be fixedly connected, first outer cylinder (352) connect with the optical filter switching mechanism (2).
4. sequencing optical system according to claim 1, which is characterized in that the optical filter switching mechanism (2) includes case
Body (21) offers light inlet (211) and two light-emitting windows (212) being oppositely arranged on the cabinet (21), and described first hides
Light sleeve (35) is set at the light inlet (211), and the object lens (41) and the imaging mechanism (6) are respectively arranged at two
At the light-emitting window (212).
5. sequencing optical system according to claim 4, which is characterized in that be provided with motor in the cabinet (21)
(22), with the gear of the output axis connection of the motor (22) and the rack gear (23) engaged with the gear, the rack gear (23)
On along the moving direction of the rack gear (23) be arranged at intervals with the first optical filter (24) and the second optical filter (25).
6. sequencing optical system according to claim 1, which is characterized in that the second shading sleeve (42) includes second
Inner cylinder and sliding sleeve are set to the second outer cylinder on second inner cylinder, second inner cylinder and the optical filter switching mechanism (2)
Connection, second outer cylinder are connect with the object lens (41).
7. sequencing optical system according to claim 1, which is characterized in that the automatic focusing mechanism (5) includes power
Motor (51) and the mobile support saddle (52) being connect with the output end of the power motor (51), the object lens (41) and the movement
Support (52) connection.
8. sequencing optical system according to claim 1, which is characterized in that the imaging mechanism (6) includes transmitting cylinder
(61), second reflection subassembly and the imager (62) are located at the both ends of transmitting cylinder (61).
9. sequencing optical system according to claim 1, which is characterized in that it further include pedestal (1), the optical filter switching
Mechanism (2), the light-transmitting means (3), the automatic focusing mechanism (5) and the imaging mechanism (6) are mounted on the base
On seat (1).
10. sequencing optical system according to claim 9, which is characterized in that the automatic focusing mechanism (5) and the base
Elastic damping component is provided between seat (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811558439.0A CN109401942A (en) | 2018-12-19 | 2018-12-19 | A kind of sequencing optical system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811558439.0A CN109401942A (en) | 2018-12-19 | 2018-12-19 | A kind of sequencing optical system |
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| Publication Number | Publication Date |
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| CN109401942A true CN109401942A (en) | 2019-03-01 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201811558439.0A Pending CN109401942A (en) | 2018-12-19 | 2018-12-19 | A kind of sequencing optical system |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110607222A (en) * | 2019-09-24 | 2019-12-24 | 广州市金圻睿生物科技有限责任公司 | Precision optical device auxiliary motion mechanism and gene sequencer |
| CN113533324A (en) * | 2020-04-16 | 2021-10-22 | 广东润鹏生物技术有限公司 | Optical detection mechanism |
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2018
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Application publication date: 20190301 |