CN109395100A - A kind of construction method of scutellarin magnetic Nano drug-loading system and application - Google Patents
A kind of construction method of scutellarin magnetic Nano drug-loading system and application Download PDFInfo
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- CN109395100A CN109395100A CN201810873466.0A CN201810873466A CN109395100A CN 109395100 A CN109395100 A CN 109395100A CN 201810873466 A CN201810873466 A CN 201810873466A CN 109395100 A CN109395100 A CN 109395100A
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Abstract
Present invention discloses a kind of construction method of scutellarin magnetic Nano drug-loading system provided by the invention and applications, in mass ratio, including 1-100 parts of ferroferric oxide nano granules, 1 part of scutellarin and ultrapure water are several；The ferroferric oxide nano granules are prepared through solvent-thermal method；Scutellarin magnetic Nano drug-loading system in the present invention, by the magnetically attractive for combining carrier ferroferric oxide nano granules；Dissolubility；Ferroferric oxide nano granules exist in the form of almost spherical, and the development of particle crystalline form is preferable, particle size, favorable dispersibility；With certain photothermal conversion property；With certain magnetic resonance imaging ability；Its good performance of physical and chemical stability at room temperature, substantially increases the biocompatibility and targeting of original scutellarin, can increase absorption of the patient to scutellarin, patient is made to obtain the effective treatment.
Description
Technical field
The present invention relates to cardiovascular and cerebrovascular diseases technical field, in particular to a kind of scutellarin magnetic Nano carries medicine system
The construction method of system and application.
Background technique
Scutellarin is a kind of chemical substance extracted in erigeron breviscapus.In light cover asarum rich in flavones and flavonoid glycoside at
Point, wherein scutellarin content a maximum of about of 1-2%, is the main active of erigeron breviscapus.Scutellarin is 7- hydroxy combining
The flavonoid glycoside of glucuronic acid base, erigeron breviscapus also known as fleabane flower are the drying herbs of compositae plant Erigeron breviscapus, are had preferable
Activating collaterals and relieving pain, dispelling wind and eliminating dampness, anti-inflammatory analgetic, expelling cold and relieving exterior syndrome, blood circulation invigorating efficacies all the time, clinic be mainly used for prevent and treat brain
Formation, cerebrovascular accident sequelae, diseases of cardiovascular and cerebrovascular systems of thrombus etc., in diseases such as diabetes, nephrosis and cervical vertigos
Treatment in also have certain booster action, between the scientific research never of cardiovascular medicament and treatment method only, with
The development and progress of medical technology, to obtain some improvement, but aggregate performance effect is there are still very big room for promotion, for into
One step improves treatment auxiliaring effect, we release construction method and the application of a kind of scutellarin magnetic Nano drug-loading system.
Summary of the invention
The object of the present invention is to provide a kind of construction method of scutellarin magnetic Nano drug-loading system and application, purports
The technical issues of solving in the prior art.
To achieve the above object, it the present invention provides a kind of construction method of scutellarin magnetic Nano drug-loading system and answers
With a kind of construction method of scutellarin magnetic Nano drug-loading system and application, it is characterised in that: in mass ratio, including four oxygen
Change 1-100 parts of three iron nano-particle, 1 part of scutellarin and ultrapure water are several；
The ferroferric oxide nano granules are prepared through solvent-thermal method.
A kind of construction method of scutellarin magnetic Nano drug-loading system and application, it is characterised in that: including such as
Lower step:
A, ferroferric oxide nano granules are dissolved in ultrapure water, are stirred well to its dissolution.
B, scutellarin is added toward above-mentioned prepare in resulting ferroso-ferric oxide solution, stirs；It is then centrifuged for handling；It removes
It is spare to collect the first lower sediment of gained for unsupported free drug.
C, obtained lower sediment will be collected with milli-Q water for several times, until supernatant is colourless, it is spare to collect supernatant,
It is to be dried overnight in 40 degrees Celsius of vacuum ovens that the second sediment of gained, which is placed in temperature, after be put into drier and save backup.
Preferably, stirring includes being mixed 0.5 hour in the step (2), is stirred by ultrasonic 0.5 hour.
Preferably, centrifugal treating selects 10000r/min in the step (2).
Beneficial effect of the invention is: scutellarin magnetic Nano drug-loading system in the present invention, by combining carrier four
The magnetically attractive of Fe 3 O nano particle；Dissolubility；Ferroferric oxide nano granules exist in the form of almost spherical, and particle
Crystalline form develops preferable, particle size, favorable dispersibility；With certain photothermal conversion property；With certain magnetic resonance imaging
Ability；Its good performance of physical and chemical stability at room temperature, substantially increases the biocompatibility of original scutellarin
And targeting, absorption of the patient to scutellarin can be increased, patient is made to obtain the effective treatment.
Detailed description of the invention
Fig. 1 is preparation and the physicochemical property research figure of ferroferric oxide magnetic nanoparticle
Fig. 2 is the transmission electron microscope picture of ferroferric oxide nano granules
Fig. 3 is the uv absorption spectra of ferroferric oxide nano granules
Fig. 4 is that ferroferric oxide nano granules solution temperature changes with time situation map
Fig. 5 is the infrared spectrogram of ferroferric oxide nano granules
Fig. 6 is the XRD spectrum of ferroferric oxide nano granules
Fig. 7 is ferriferrous oxide nano aqueous solution magnetic resonance radiography image
Three-dismensional effect face figure of the Fig. 8 between each factor
Fig. 9 is 25 DEG C of scutellarin-ferroferric oxide nano granules In-vitro release curves
Figure 10 is 37 DEG C of scutellarin-ferroferric oxide nano granules In-vitro release curves
Figure 11 is 45 DEG C of scutellarin-ferroferric oxide nano granules In-vitro release curves
Specific embodiment
Embodiment 1
A kind of construction method of scutellarin magnetic Nano drug-loading system and application: in mass ratio, including ferroso-ferric oxide
1-100 parts of nano particle, 1 part of scutellarin and ultrapure water are several；
The ferroferric oxide nano granules are prepared through solvent-thermal method.
A kind of construction method of scutellarin magnetic Nano drug-loading system and application, include the following steps:
A, ferroferric oxide nano granules are dissolved in ultrapure water, are stirred well to its dissolution.
B, scutellarin is added toward above-mentioned prepare in resulting ferroso-ferric oxide solution, stirs；It is then centrifuged for handling；It removes
It is spare to collect the first lower sediment of gained for unsupported free drug.
C, obtained lower sediment will be collected with milli-Q water for several times, until supernatant is colourless, it is spare to collect supernatant,
It is to be dried overnight in 40 degrees Celsius of vacuum ovens that the second sediment of gained, which is placed in temperature, after be put into drier and save backup.
Embodiment 2
A kind of construction method of scutellarin magnetic Nano drug-loading system and application: in mass ratio, including ferroso-ferric oxide
1 part of nano particle, 1 part of scutellarin and ultrapure water are several；
The ferroferric oxide nano granules are prepared through solvent-thermal method.
A kind of construction method of scutellarin magnetic Nano drug-loading system and application, include the following steps:
A, ferroferric oxide nano granules are dissolved in ultrapure water, are stirred well to its dissolution.
B, scutellarin is added toward above-mentioned prepare in resulting ferroso-ferric oxide solution, stirs；It is then centrifuged for handling；It removes
It is spare to collect the first lower sediment of gained for unsupported free drug.
C, obtained lower sediment will be collected with milli-Q water for several times, until supernatant is colourless, it is spare to collect supernatant,
It is to be dried overnight in 40 degrees Celsius of vacuum ovens that the second sediment of gained, which is placed in temperature, after be put into drier and save backup.
Embodiment 3
A kind of construction method of scutellarin magnetic Nano drug-loading system and application: in mass ratio, including ferroso-ferric oxide
10 parts of nano particle, 1 part of scutellarin and ultrapure water are several；
The ferroferric oxide nano granules are prepared through solvent-thermal method.
A kind of construction method of scutellarin magnetic Nano drug-loading system and application, include the following steps:
A, ferroferric oxide nano granules are dissolved in ultrapure water, are stirred well to its dissolution.
B, scutellarin is added toward above-mentioned prepare in resulting ferroso-ferric oxide solution, stirs；It is then centrifuged for handling；It removes
It is spare to collect the first lower sediment of gained for unsupported free drug.
C, obtained lower sediment will be collected with milli-Q water for several times, until supernatant is colourless, it is spare to collect supernatant,
It is to be dried overnight in 40 degrees Celsius of vacuum ovens that the second sediment of gained, which is placed in temperature, after be put into drier and save backup.
Embodiment 4
A kind of construction method of scutellarin magnetic Nano drug-loading system and application: in mass ratio, including ferroso-ferric oxide
30 parts of nano particle, 1 part of scutellarin and ultrapure water are several；
The ferroferric oxide nano granules are prepared through solvent-thermal method.
A kind of construction method of scutellarin magnetic Nano drug-loading system and application, include the following steps:
A, ferroferric oxide nano granules are dissolved in ultrapure water, are stirred well to its dissolution.
B, scutellarin is added toward above-mentioned prepare in resulting ferroso-ferric oxide solution, stirs；It is then centrifuged for handling；It removes
It is spare to collect the first lower sediment of gained for unsupported free drug.
C, obtained lower sediment will be collected with milli-Q water for several times, until supernatant is colourless, it is spare to collect supernatant,
It is to be dried overnight in 40 degrees Celsius of vacuum ovens that the second sediment of gained, which is placed in temperature, after be put into drier and save backup.
Embodiment 5
A kind of construction method of scutellarin magnetic Nano drug-loading system and application: in mass ratio, including ferroso-ferric oxide
40 parts of nano particle, 1 part of scutellarin and ultrapure water are several；
The ferroferric oxide nano granules are prepared through solvent-thermal method.
A kind of construction method of scutellarin magnetic Nano drug-loading system and application, include the following steps:
A, ferroferric oxide nano granules are dissolved in ultrapure water, are stirred well to its dissolution.
B, scutellarin is added toward above-mentioned prepare in resulting ferroso-ferric oxide solution, stirs；It is then centrifuged for handling；It removes
It is spare to collect the first lower sediment of gained for unsupported free drug.
C, obtained lower sediment will be collected with milli-Q water for several times, until supernatant is colourless, it is spare to collect supernatant,
It is to be dried overnight in 40 degrees Celsius of vacuum ovens that the second sediment of gained, which is placed in temperature, after be put into drier and save backup.
Embodiment 6
A kind of construction method of scutellarin magnetic Nano drug-loading system and application: in mass ratio, including ferroso-ferric oxide
60 parts of nano particle, 1 part of scutellarin and ultrapure water are several；
The ferroferric oxide nano granules are prepared through solvent-thermal method.
A kind of construction method of scutellarin magnetic Nano drug-loading system and application, include the following steps:
A, ferroferric oxide nano granules are dissolved in ultrapure water, are stirred well to its dissolution.
B, scutellarin is added toward above-mentioned prepare in resulting ferroso-ferric oxide solution, stirs；It is then centrifuged for handling；It removes
It is spare to collect the first lower sediment of gained for unsupported free drug.
C, obtained lower sediment will be collected with milli-Q water for several times, until supernatant is colourless, it is spare to collect supernatant,
It is to be dried overnight in 40 degrees Celsius of vacuum ovens that the second sediment of gained, which is placed in temperature, after be put into drier and save backup.
Embodiment 7
A kind of construction method of scutellarin magnetic Nano drug-loading system and application: in mass ratio, including ferroso-ferric oxide
80 parts of nano particle, 1 part of scutellarin and ultrapure water are several；
The ferroferric oxide nano granules are prepared through solvent-thermal method.
A kind of construction method of scutellarin magnetic Nano drug-loading system and application, include the following steps:
A, ferroferric oxide nano granules are dissolved in ultrapure water, are stirred well to its dissolution.
B, scutellarin is added toward above-mentioned prepare in resulting ferroso-ferric oxide solution, stirs；It is then centrifuged for handling；It removes
It is spare to collect the first lower sediment of gained for unsupported free drug.
C, obtained lower sediment will be collected with milli-Q water for several times, until supernatant is colourless, it is spare to collect supernatant,
It is to be dried overnight in 40 degrees Celsius of vacuum ovens that the second sediment of gained, which is placed in temperature, after be put into drier and save backup.
Embodiment 8
A kind of construction method of scutellarin magnetic Nano drug-loading system and application: in mass ratio, including ferroso-ferric oxide
100 parts of nano particle, 1 part of scutellarin and ultrapure water are several；
The ferroferric oxide nano granules are prepared through solvent-thermal method.
A kind of construction method of scutellarin magnetic Nano drug-loading system and application, include the following steps:
A, ferroferric oxide nano granules are dissolved in ultrapure water, are stirred well to its dissolution.
B, scutellarin is added toward above-mentioned prepare in resulting ferroso-ferric oxide solution, stirs；It is then centrifuged for handling；It removes
It is spare to collect the first lower sediment of gained for unsupported free drug.
C, obtained lower sediment will be collected with milli-Q water for several times, until supernatant is colourless, it is spare to collect supernatant,
It is to be dried overnight in 40 degrees Celsius of vacuum ovens that the second sediment of gained, which is placed in temperature, after be put into drier and save backup.
Embodiment 9
A kind of construction method of scutellarin magnetic Nano drug-loading system and application: including ferroferric oxide nano granules
48.02mg, scutellarin 56.53mg and ultrapure water 120mL；
The ferroferric oxide nano granules are prepared through solvent-thermal method.
A kind of construction method of scutellarin magnetic Nano drug-loading system and application, include the following steps:
A, ferroferric oxide nano granules are dissolved in ultrapure water, are stirred well to its dissolution.
B, scutellarin is added toward above-mentioned prepare in resulting ferroso-ferric oxide solution, stirs；It is then centrifuged for handling；It removes
It is spare to collect the first lower sediment of gained for unsupported free drug.
C, obtained lower sediment will be collected with milli-Q water for several times, until supernatant is colourless, it is spare to collect supernatant,
It is to be dried overnight in 40 degrees Celsius of vacuum ovens that the second sediment of gained, which is placed in temperature, after be put into drier and save backup.
Implement related experimental methods and analysis of experimental results in 9:
One, the preparation of ferroferric oxide nano granules:
It is surface modified with polyethylene glycol 2000, ferroferric oxide nano granules is prepared by solvent-thermal method.Its
Concrete operations are as follows: a certain amount of Iron(III) chloride hexahydrate powder being taken to be dissolved in 40ml mixed solvent (20ml ethylene glycol and 20ml
Ethanol amine) in, it forms stable orange solution and sodium acetate and polyethylene glycol 2000 is added after agitated ultrasound half an hour.It is mixed
After closing uniformly, solution is transferred in polytetrafluoroethylene (PTFE) stainless steel autoclave, heated sealed is to 200 DEG C.After reacting 10h,
Autoclave cooled to room temperature through ethyl alcohol and is gone to get ferroferric oxide nano granules using the suction-operated of magnet
After ionized water washs in order, save backup.The wherein amount of taking point of Iron(III) chloride hexahydrate, sodium acetate, polyethylene glycol 2000
It Wei not 1~3g, 1~3g, 0.5~1.5g.
Fig. 1 reflects ferroferric oxide nano granules with magnetically attractive (A: ferroferric oxide nano granules aqueous solution；B: will
Magnet is placed in bottle wall；C: magnet is removed)；As shown in Figure 1, the ferroferric oxide nano granules being prepared are in black, have one
Fixed water solubility, when magnet is close to ferroferric oxide nano granules aqueous solution, ferroferric oxide nano granules are towards magnet direction
Mobile (such as Figure 1B), is finally collected at magnet set-down location (such as Fig. 1 C), shows that the ferroferric oxide nano granules being prepared have
There is certain magnetically attractive.
From Figure 2 it can be seen that carrying out morphologic observation, ferriferrous oxide nano to ferroferric oxide nano granules using transmission electron microscope
Particle exists in the form of almost spherical, and the development of particle crystalline form is preferable, particle size, favorable dispersibility.
As shown in figure 3, finding four after investigating to the ultra-violet absorption spectrum of various concentration ferroferric oxide nano granules
Fe 3 O nano particle has certain suction in visible light (400-700nm) and near infrared light (800-1100nm) range
It receives, as the situation risen afterwards first drops in the red shift presentation of wavelength, occurs minimum at 900nm, reflect four to a certain extent
Fe 3 O nano particle in the near infrared region in may have certain photothermal conversion property.
It is not studied in various concentration or not by irradiating ferroferric oxide nano granules using 808nm semiconductor laser
It changes with time situation with temperature under illumination intensity illumination.As shown in fig. 4 a, when intensity of illumination is fixed as 2W/cm2When, with
Ferroferric oxide nano granules concentration increases, and the elevation amplitude of temperature is also increasing, but amplification is not obvious.But as long as
A small amount of ferroferric oxide nano granules (0.102mg/ml) is added, rising sharply for temperature will be caused.It can be seen that from Fig. 4 b
When ferroferric oxide nano granules concentration is 1.01mg/ml, with gradually rising for intensity of illumination, gradient of temperature amplitude is step by step
Increase, variation is obvious, and showing ferroferric oxide nano granules really has certain photothermal conversion property.
Ferroferric oxide nano granules temperature changes with time situation；B: concentration is that 1.01mg/ml ferroso-ferric oxide is received
Rice grain temperature under different illumination intensity changes with time situation), it is contemplated that ferroferric oxide nano granules have certain
Magnetic thermal property, therefore we also examine the magnetic thermal property for the ferroferric oxide nano granules that different ratio is prepared
It examines, discovery ferroferric oxide nano granules are no more than 2 DEG C since the thermogenetic temperature of magnetic increases, ferriferrous oxide nano
The raised variation of temperature occurs under laser irradiation and is mainly generated by photothermal conversion property for grain, and increases the amplitude size of temperature
Related with the particle size of ferroferric oxide nano granules, specific experimental data is shown in document " Jun Hu, Ashkan
Dehsorkhi,Wafa T.Al-Jamal, Yan Zhang,Siying Chen,Chuanyu Yang,Dan Tan,Qing
Zhao,Chang Yang*, Yonglin Wang*,Studies on the photothermal effect of
PEGylated ferroso-ferric oxide nanoparticles, Nanoscience andNanotechnology Letters, 2017,
9(4):553-558”。
What Fig. 5 reflected is the infrared spectrogram of ferroferric oxide nano granules.As can be seen from the figure ferroso-ferric oxide is received
Rice grain is in 579cm-1There is Fe-O characteristic peak, there is the stretching vibration peak of C=O at 1591cm-1, there is poly- second at 3412cm-1
The peak-OH of glycol molecules, the common presence of three of the above characteristic peak, which shows ferroferric oxide nano granules surface, PEG molecule
In the presence of, and the two bonding is good.
Fig. 6 is the X ray diffracting spectrum (X-ray diffraction, XRD) of ferroferric oxide nano granules.From figure
As can be seen that sample spectral line goes out at 2 θ=30.11 °, 35.50 °, 43.32 °, 53.75 °, 57.06 °, 62.68 °, 74.49 °
Existing diffraction maximum, it is corresponding with (220), (311), (400), (422), (511), (440), (533) crystal face respectively, the result with
Ferroso-ferric oxide PDF standard card (JCPDS 01-085-1436) unanimously, shows to contain ferroso-ferric oxide crystal phase in sample.
We are by Philips Achieva 3.0T MRI scan instrument to 0.1mg/ml ferriferrous oxide nano
Particle aqueous solution has carried out T2Scanning imagery, as a result as shown in fig. 7, T can be enhanced in ferroferric oxide nano granules2Signal has
Certain magnetic resonance imaging ability.
2. the study on the stability of ferroferric oxide magnetic nanoparticle
Ferroferric oxide nano granules are stored in drier, is taken out when 0 day, 1 month, 6 months and is made four
Fe 3 O nanoparticles solution evaluates using its particle size of tem observation ferroferric oxide nano granules at room temperature
Physical and chemical stability.The results are shown in Table 1 for it, and ferroferric oxide nano granules particle size variation under room temperature state is unknown
It is aobvious, it was demonstrated that physical and chemical stability is good at room temperature for it.
1. ferriferrous oxide nano grain physical and chemical stability of table investigates result table
3. utilizing the prescription of Box-Behnken effect surface model optimization ferroferric oxide magnetic nanoparticle
According to Box-Behnken experimental design principles, the investment of ferric trichloride, sodium acetate and polyethylene glycol 2000 is chosen
Amount is independent variable, using Box-Behnken design experiment.Its design factor and level are shown in Table 2.
Table 2.Box-Behnken experimental design factor and level
Wherein Box-Behnken design scheme and it the results are shown in Table 3.
Table 3.Box-Behnken design scheme and result
Using solubility values as effect value (Y), multiple regression point is carried out to data using Design-Expert 8.0.6 software
Analysis, obtains regression equation
Y=42.20+0.35X1+0.70X2+5.49X3-1.87X1X2+3.50X1X3+7.94X2X3+5.49X1 2-1
6.22X2 2+15.29X3 2(R2=0.9158), the results of analysis of variance of each factor is shown in Table 4, the three-dismensional effect face figure between each factor
See Fig. 8.
As shown in Table 4, model P=0.0309, otherness is significant, has statistical significance.By the three-dimensional effect between each factor
It is 3.00g that answer face figure (Fig. 8) that optimal prescription proportion can be predicted, which be ferric trichloride, sodium acetate 2.21g, and polyethylene glycol 2000 is
1.50g。
4. the results of analysis of variance of table
| Source | Quadratic sum | Freedom degree | Mean square deviation | F |
| Model | 2663.36 | 9 | 295.93 | 6.05 |
| X1- ferric trichloride | 0.98 | 1 | 0.98 | 0.02 |
| X2- sodium acetate | 3.92 | 1 | 3.92 | 0.08 |
| X3- polyethylene glycol 2000 | 240.74 | 1 | 240.74 | 4.92 |
| X1X2 | 13.95 | 1 | 13.95 | 0.28 |
| X1X3 | 49.04 | 1 | 49.04 | 1 |
| X2X3 | 251.96 | 1 | 251.96 | 5.15 |
| X12 | 111.11 | 1 | 111.11 | 2.27 |
| X22 | 971.82 | 1 | 971.82 | 19.85 |
| X32 | 863.18 | 1 | 863.18 | 17.63 |
| Residual error | 244.77 | 5 | 48.95 | |
| It is quasi- poor to lose | 211.64 | 3 | 70.55 | |
| Pure error | 33.13 | 2 | 16.57 | |
| Summation | 2908.13 | 14 |
Experimental summary: detection and analysis experiment is carried out by preparing ferroferric oxide nano granules, and to it, can be learnt
Ferroferric oxide nano granules have certain magnetically attractive；Dissolubility is stronger；Ferroferric oxide nano granules are with almost spherical
Form exists, and the development of particle crystalline form is preferable, particle size, favorable dispersibility；Ferroferric oxide nano granules are near infrared region
There is certain photothermal conversion property in domain；With certain magnetic resonance imaging ability；Its physical and chemical stability at room temperature
The features such as good.
Two, the preparation of scutellarin magnetic nanoparticle
48.02mg ferroferric oxide nano granules are dissolved in 120ml ultrapure water, stirring to its dissolution.56.53mg is added
0.5h is mixed in scutellarin, and 0.5h is stirred by ultrasonic；Then 10000r/min is centrifuged, and removes unsupported free drug lamp
Small cup B prime collects the first sediment of gained, and with milli-Q water for several times until supernatant is completely colorless, it is total that supernatant is collected in merging
110ml measures absorbance value in 335nm with ultraviolet spectrophotometry, calculates drugloading rate and encapsulation rate according to standard curve.Most
The second sediment of gained is placed in 40 DEG C of vacuum ovens afterwards and is dried overnight, rear taking-up, which is put into drier, to be saved backup.
A. the vitro release measurement of scutellarin magnetic nanoparticle
Second sediment is configured to the solution of 1mg/ml, 15ml is taken to be fitted into processed bag filter, at 25 DEG C, 37
DEG C, dialyse under 45 DEG C (100r/min), respectively 0.5,1,2,4,6,8,10,12, point in time sampling 5ml for 24 hours, simultaneously
The fresh dissolution medium for supplementing same volume takes part dialysis solution to measure absorbance value in 335nm, calculates release percentage.
B. the preparation of standard curve
5.12mg scutellarin is weighed in 50ml volumetric flask, is dissolved with ultrapure water water, constant volume mixes, as stock solution.
Respectively draw 2,10,50,250,750,3750ul stock solution in the volumetric flask of 100ml, ultrapure water is settled to graduation mark.Take part
Solution measures absorbance value at 335nm, and standard curve is calculated according to absorbance value A and solution concentration C.
As a result
A. standard curve: A=59.668C+0.0002 (R2=0.9998)
| Concentration C (mg/mL) | 0.000002 | 0.00001 | 0.00005 | 0.00025 | 0.00075 | 0.00375 |
| Absorbance A | 0.0007 | 0.0013 | 0.0035 | 0.016 | 0.0427 | 0.2244 |
C. encapsulation rate and drugloading rate
The supernatant of 110ml is diluted 100 times, takes and measures absorbance value at 335nm on a small quantity.
C=0.000533 is calculated according to standard curve in A=0.032, so m=5.86mg, i.e. free drug amount are
5.86mg。
D. cumulative in vitro burst size
E. the fitting of mathematical model
Scutellarin-ferroferric oxide nano granules drug accumulation release percentage is quasi- from different models at 5. 25 DEG C of table
The result of conjunction
Scutellarin-ferroferric oxide nano granules drug accumulation release percentage is quasi- from different models at 6. 37 DEG C of table
The result of conjunction
Scutellarin-ferroferric oxide nano granules drug accumulation release percentage is quasi- from different models at 7. 45 DEG C of table
The result of conjunction
It can be obtained by above-mentioned experiment, in the construction method of scutellarin magnetic Nano drug-loading system, the encapsulating of scutellarin
Rate is up to 89.63%, and drugloading rate is up to 53.82%；Scutellarin magnetic Nano drug-loading system is external over time
Burst size scutellarin magnetic Nano drug-loading system in explosive increase, the present invention, by being received in conjunction with carrier ferroso-ferric oxide
The magnetically attractive of rice grain；Dissolubility；Ferroferric oxide nano granules exist in the form of almost spherical, and particle crystalline form development compared with
Good, particle size, favorable dispersibility；With certain photothermal conversion property；With certain magnetic resonance imaging ability；It is in room
The lower good performance of physical and chemical stability of temperature, substantially increases the biocompatibility and targeting of original scutellarin, can
To increase absorption of the patient to scutellarin, patient is made to obtain the effective treatment.
Above disclosed is only a preferred embodiment of the present invention, cannot limit the power of the present invention with this certainly
Sharp range, those skilled in the art can understand all or part of the processes for realizing the above embodiment, and weighs according to the present invention
Benefit requires made equivalent variations, still belongs to the scope covered by the invention.
Claims (4)
1. construction method and the application of a kind of scutellarin magnetic Nano drug-loading system, it is characterised in that: in mass ratio, including four
1-100 parts of Fe 3 O nano particle, 1 part of scutellarin and ultrapure water are several；
The ferroferric oxide nano granules are prepared through solvent-thermal method.
2. construction method and the application of a kind of scutellarin magnetic Nano drug-loading system according to claim 1, feature
It is: includes the following steps:
A, ferroferric oxide nano granules are dissolved in ultrapure water, are stirred well to its dissolution.
B, scutellarin is added toward above-mentioned prepare in resulting ferroso-ferric oxide solution, stirs；It is then centrifuged for handling；Removing is not born
It is spare to collect the first lower sediment of gained for the free drug of load.
C, obtained lower sediment will be collected with milli-Q water for several times, until supernatant is colourless, it is spare to collect supernatant, gained
It is to be dried overnight in 40 degrees Celsius of vacuum ovens that second sediment, which is placed in temperature, after be put into drier and save backup.
3. construction method and the application of a kind of scutellarin magnetic Nano drug-loading system according to claim 1, feature
Be: stirring includes being mixed 0.5 hour in the step (2), is stirred by ultrasonic 0.5 hour.
4. a kind of scutellarin magnetic Nano drug-loading system according to claim 2, it is characterised in that: the step (2)
Middle centrifugal treating selects 10000r/min.
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| CN112007035A (en) * | 2020-09-02 | 2020-12-01 | 贵州医科大学 | Magnetic carrier for targeted medicine and preparation method thereof |
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