CN109385461A - A kind of duck diet external source zymogram optimization method - Google Patents
A kind of duck diet external source zymogram optimization method Download PDFInfo
- Publication number
- CN109385461A CN109385461A CN201811230125.8A CN201811230125A CN109385461A CN 109385461 A CN109385461 A CN 109385461A CN 201811230125 A CN201811230125 A CN 201811230125A CN 109385461 A CN109385461 A CN 109385461A
- Authority
- CN
- China
- Prior art keywords
- diet
- enzyme
- simulation
- duck
- zymogram
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/40—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving amylase
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Enzymes And Modification Thereof (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The present invention relates to a kind of duck diet external source zymogram optimization methods, include the following steps: the parsing of diet protein and carbohydrate ingredient, the setting of exogenous enzymes type and dosage, diet simulation peptic digest, diet simulation intestinal digestion, the optimization of external source zymogram.The present invention uses carbohydrate ingredient analytical technology and in vitro digestion technology, and the function and effect of exogenous enzymes, the optimal zymogram added in quick obtaining duck diet are characterized using the external effective energy value of feed.
Description
Technical field
The present invention relates to a kind of duck diet external source zymogram optimization methods, more particularly to utilization diet carbohydrate ingredient
The method of parsing and in vitro digestion technology rapid Optimum duck diet external source zymogram.
Background technique
Protein is the important component of body and livestock products, and carbohydrate is the main part of feed.In Sino-U.S.
Under trade war background, feedstuff cost continuous rise, consumer seeks less expensive unconventional feed part substitution and passes
The corn and bean-dregs feed raw material of system.However, these unconventional feed often thick protein rich in and higher carbon
Hydrate, and unconventional water resources material carbon hydrate cannot be made full use of by animal body (Anguita et al.,
2006).Currently, generally use addition exogenous protease come improve protein utilization and add external source carbohydrase come
The digestion for improving dietary fiber, as zytase (Engberg et al., 2006), cellulase (Nahm et al.,
1985), 1,4 beta-glucanase (Yu et al., 1998) etc., and the function and effect of exogenous enzymes and diet composition, the activity of enzyme, zymogram
Compatibility is closely related.However, the uncertain factors such as the type of exogenous enzymes and compatibility, cause at present generally to have the addition of exogenous enzymes
There is certain blindness.
The method for screening the optimal zymogram of feed mainly has in vivo method (in vivo) and in vitro method (in vitro).In vivo
Method is exactly to carry out animal experiment, usually cost, laborious, time-consuming, systematic error and accidental error influence factor it is more (Chen Liang etc.,
2013).And in vitro method has the advantages that quick, easy, expense is low etc., but the parameter of in vitro method selection at present and condition usually lack
Theoretical explanation, with certain randomness (Coles et al., 2005).Therefore, in parsing diet protein and carbon aquation
On the basis of polymer component, reasonable in vitro digestion parameter is chosen, simulates the digestion process of duck diet, is raised for different ducks
Grain protein and carbohydrate component characteristic, the protease and carbohydrate zymogram that screening is adapted therewith, for abundant
Feed potential is played, efficiency of feed utilization is improved and increase breeding production benefit is of great significance.
Summary of the invention
Technology of the invention solves the problems, such as: in order to overcome the blindness for adding exogenous enzymes in current duck diet, providing one kind
The fast Optimization of duck diet external source zymogram is simulated by parsing duck diet protein and carbohydrate ingredient and in vitro method
Animal digestion process uses the function and effect of the external effective energy value characterization exogenous enzymes of diet, the optimal zymogram of quick obtaining duck diet.
Technical solution of the invention is as follows: duck diet external source zymogram optimization method comprises the following steps that
(1) it the parsing of diet protein and carbohydrate ingredient: chooses duck diet and is put into 3~5 points of crushing in pulverizer
Clock；Smashed feed is crossed into 40~60 meshes；- 20 DEG C are placed in store for future use；125~200 milligrams of duck diet are weighed, duck is measured
The content of diet protein and carbohydrate ingredient；
(2) setting of exogenous enzyme preparations type and dosage: according to the duck diet sample protein matter and carbohydrate
Component according to principal component analysis as a result, weighing 1.8~2.2 grams of duck diet, and adds 1~2 kind of albumen in the duck diet
Enzyme and 3~5 kinds of carbohydrases, the additive capacity setting 0 of every kind of enzyme preparation, 50,100,150,200,250,300,350,
400 μ g/g series of concentrations gradients, prepare and are added to the enzyme diet of various concentration；
(3) it simulates peptic digest: the sample of the enzyme diet is placed in triangular flask, and simulation stomach is added in triangular flask
Buffer is digested, the simulation peptic digest buffer is pH 2.0~3.0, and molar concentration rate is (16~17): the chlorine of 10:10
Change sodium: potassium chloride: hydrochloric acid solution；700~800U/mL stomach cardia enzyme solution is added simultaneously；Wherein enzyme diet, simulation peptic digest are slow
Fliud flushing stomach function regulating liquid of protease is according to (2~4): 20:1 weight ratio is uniformly mixed；It will be equipped with uniformly mixed enzyme diet, simulation stomach
Digestion buffer stomach function regulating liquid of protease triangular flask be placed in water bath with thermostatic control shaking table, set water bath with thermostatic control shaking table revolving speed 170~
190rpm and 41~43 DEG C of temperature carry out 4~6h and simulate peptic digest；
(4) simulate intestinal digestion: after to the simulation peptic digest, molar concentration rate, which is added, in the triangular flask is
(13~18): the sodium chloride of 3:40: potassium chloride: phosphatic simulation intestinal digestion buffer；PH 6.2~6.6 is added simultaneously, it is single
Position enzyme activity ratio (85~90): 40:300:30:5:1=trypsase: chymotrypsin: amylase: maltose: invertase: rouge
The simulation intestinal digestion enzyme solution of fat enzyme；Wherein the weight percentage ranges of simulation intestinal digestion buffer and simulation intestinal digestion enzyme solution are
55:1~60:1；Continue set water bath with thermostatic control shaking table 170~190rpm of revolving speed and 41~43 DEG C of temperature, carry out 12~16h mould
The digestion of quasi- intestines；
(5) optimization of external source zymogram: after simulating digestion, by slaking residue, drying to constant weight, and measurement residue is external
Effective energy value calculates the external effective energy value of diet of addition every kind of exogenous protease and carbohydrase, and according to external source
The regression relation of enzyme and external effective energy value show that every kind of exogenous enzymes can improve the critical of the external effective energy value of diet of target
Point；When every kind of exogenous enzymes are encoded according to the critical point, according to the type of a variety of exogenous enzymes of addition, corresponding number is set
5 levels are arranged in the impact factor of amount, each impact factor, analyze external available energy using regression rotation Orthogonal Composite Design
Value, and using external effective energy value and exogenous enzymes additive capacity relationship, symbolize optimal external source zymogram.
The advantages of the present invention over the prior art are that:
(1) present invention is using experimental data in a large amount of animal bodies as foundation, and the in-vitro simulated parameter of selection is more close to animal
Practical physiological status, have the effect of preferably simulating diet digestion process in duck body；
(2) the present invention overcomes the blindness of previous exogenous enzyme preparation, in parsing duck diet protein and carbon aquation
On the basis of polymer component, efficiently external means simulate feed in the digestion process of chicken gastrointestinal tract for use, and propose have in vitro
Efficiency value characterizes optimal zymogram, has achieved the purpose that rapid Optimum external source zymogram.
Detailed description of the invention
Fig. 1 is the flow chart that the present invention realizes.
Specific embodiment
With reference to the accompanying drawing and the present invention is discussed in detail in specific embodiment.But embodiment below is only limitted to explain this hair
Bright, protection scope of the present invention should include the full content of claim, be not limited only to the present embodiment.
Embodiment 1,
As shown in Figure 1, concrete operation method of the present invention can be implemented as follows:
It chooses corn-soybean meal diet to be put into pulverizer, crush 3~5 minutes；Smashed feed is crossed into 40~60 mesh
Sieve；- 20 DEG C are placed in store for future use；125~200 milligrams of corn-soybean meal diets are weighed, diet protein and carbon hydrate are measured
The content of object component；
According to the diet sample protein matter and carbohydrate ingredient, according to principal component analysis as a result, weighing corn-
1.8~2.2 grams of dregs of beans diet, and a kind of protease (acidic protein) and 3 kinds of carbohydrate are added in the duck diet
Enzyme (zytase, cellulase, 1,4 beta-glucanase), the additive capacity setting 0 of every kind of enzyme preparation, 50,100,150,200,
250,300,350,400 μ g/g series of concentrations gradient, prepares the jade for being added to various concentration protease and carbohydrase
Rice-dregs of beans diet；
The sample of the enzyme diet is placed in triangular flask, and simulation peptic digest buffer, institute are added in triangular flask
The simulation peptic digest buffer stated is pH 2.0~3.0, and molar concentration rate is (16~17): the sodium chloride of 10:10: potassium chloride:
Hydrochloric acid solution；700~800U/mL stomach cardia enzyme solution is added simultaneously；Wherein enzyme diet, simulation peptic digest buffer and stomach cardia
Enzyme solution is according to (2~4): 20:1 weight ratio is uniformly mixed；Will equipped with uniformly mixed enzyme diet, simulation peptic digest buffer and
The triangular flask of stomach cardia enzyme solution is placed in water bath with thermostatic control shaking table, set shaking table 170~190rpm of revolving speed and 41~43 DEG C of temperature,
4~6h of digestion time；
After to the simulation peptic digest, it is (13~18) that molar concentration rate, which is added, in the triangular flask: 3:40's
Sodium chloride: potassium chloride: phosphatic simulation intestinal digestion buffer；While pH 6.2~6.6 is added, unit enzyme activity ratio (85~
90): 40:300:30:5:1=trypsase: chymotrypsin: amylase: maltose: invertase: the simulation intestines of lipase disappear
Change enzyme solution；Wherein the weight percentage ranges of simulation intestinal digestion buffer and simulation intestinal digestion enzyme solution are (55~60): 1；Continue
Set shaking table 170~190rpm of revolving speed and 41~43 DEG C of temperature, 12~16h of digestion time；
After simulating digestion, by slaking residue, drying to constant weight, measures the external effective energy value of residue, calculates addition
The external effective energy value of the diet of every kind of exogenous protease and carbohydrase, and according to time of exogenous enzymes and external effective energy value
Return relationship, show that every kind of exogenous enzymes can improve the critical point of the external effective energy value of diet of target；By every kind of exogenous enzymes according to institute
When stating critical point and being encoded, according to the type of a variety of exogenous enzymes of addition, the impact factor of corresponding number, each influence are set
5 levels (table 1) are arranged in the factor, analyze external effective energy value using quaternary quadratic regression rotating orthogonal Combination Design:
=a0+a1x1+a2x2+a3x3+a4x4+a12x1x2+a13x1x3+a14x1x4+a23x2x3+a24x2x4+a34x3x4
+a11x1 2+a22x2 2+a33x3 2+a44x4 2(a, regression equation coefficient；x1, acid protease；x2, zytase；x3, fine
Tie up plain enzyme；x4, 1,4 beta-glucanase).It is combined using the non-starch polysaccharide zymogram in partial derivative method solution regression equation and each
Non-starch polysaccharide enzyme additive capacity.
Table 1
| Encoded radio | Acidic protein | Zytase | Cellulase | 1,4 beta-glucanase |
| 2.378 | 50μg/g | 200μg/g | 50μg/g | 50μg/g |
| 1 | 100μg/g | 250μg/g | 100μg/g | 100μg/g |
| 0 | 150μg/g | 300μg/g | 150μg/g | 150μg/g |
| -1 | 200μg/g | 350μg/g | 200μg/g | 200μg/g |
| -2.378 | 250μg/g | 400μg/g | 250μg/g | 250μg/g |
Embodiment 2,
It chooses wheat-miscellaneous meal type diet to be put into pulverizer, crush 3~5 minutes；Smashed feed is crossed into 40~60 mesh
Sieve；- 20 DEG C are placed in store for future use；125~200 milligrams of wheats-miscellaneous meal type diet is accurately weighed, diet protein and carbon water are measured
The content of compound component；
According to the diet sample protein matter and carbohydrate ingredient, according to principal component analysis as a result, weighing wheat-
1.8~2.2 grams of miscellaneous meal type diet, and 2 kinds of protease (acidic protein and neutral proteinase) are added in the duck diet
With 4 kinds of carbohydrases (zytase, cellulase, 1,4 beta-glucanase, arabanase), the addition of every kind of enzyme preparation
0,50,100,150,200,250,300,350,400 μ g/g series of concentrations gradients are arranged in dosage, prepare a series of be added to not
With the wheat of concentration protein enzyme and carbohydrase-miscellaneous meal type diet；
The sample of the enzyme diet is placed in triangular flask, and simulation peptic digest buffer, institute are added in triangular flask
The simulation peptic digest buffer stated is pH 2.0~3.0, and molar concentration rate is (16~17): the sodium chloride of 10:10: potassium chloride:
Hydrochloric acid solution；700~800U/mL stomach cardia enzyme solution is added simultaneously；Wherein enzyme diet, simulation peptic digest buffer and stomach cardia
Enzyme solution is according to (2~4): 20:1 weight ratio is uniformly mixed；Will equipped with uniformly mixed enzyme diet, simulation peptic digest buffer and
The triangular flask of stomach cardia enzyme solution is placed in water bath with thermostatic control shaking table, set shaking table 170~190rpm of revolving speed and 41~43 DEG C of temperature,
4~6h of digestion time；
After to the simulation peptic digest, it is (13~18) that molar concentration rate, which is added, in the triangular flask: 3:40's
Sodium chloride: potassium chloride: phosphatic simulation intestinal digestion buffer；While pH 6.2~6.6 is added, unit enzyme activity ratio (85~
90): 40:300:30:5:1=trypsase: chymotrypsin: amylase: maltose: invertase: the simulation intestines of lipase disappear
Change enzyme solution；Wherein the weight percentage ranges of simulation intestinal digestion buffer and simulation intestinal digestion enzyme solution are (55~60): 1；Continue
Set shaking table 170~190rpm of revolving speed and 41~43 DEG C of temperature, 12~16h of digestion time；
After simulating digestion, by slaking residue, drying to constant weight, measures the external effective energy value of residue, calculates addition
The external effective energy value of the diet of every kind of exogenous protease and carbohydrase, and according to time of exogenous enzymes and external effective energy value
Return relationship, show that every kind of exogenous enzymes can improve the critical point of the external effective energy value of diet of target；By every kind of exogenous enzymes according to institute
When stating critical point and being encoded, according to the type of a variety of exogenous enzymes of addition, the impact factor of corresponding number, each influence are set
5 levels (table 2) are arranged in the factor, analyze external effective energy value using quaternary quadratic regression rotating orthogonal Combination Design:
=a0+a1x1+a2x2+a3x3+a4x4+a5x5+a6x6+a12x1x2+a13x1x3+a14x1x4+a15x1x5+a16x1x6+
a23x2x3+a24x2x4+a25x2x5+a26x2x6+a34x3x4+a35x3x5+a36x3x6+a45x4x5+a46x4x6+a56x5x6+a11x1 2+a22x2 2
+a33x3 2+a44x4 2+a55x5 2+a66x6 2(a, regression equation coefficient；x1, acid protease；x2, neutral proteinase；x3, xylan
Enzyme；x3, cellulase；x5, 1,4 beta-glucanase；x6, arabinoxylanase).It is solved using partial derivative method outer in regression equation
The combination of source zymogram and each exogenous enzymes additive capacity.
Table 2
| Encoded radio | Acidic protein | Neutral protein | Zytase | Cellulase | 1,4 beta-glucanase | Arabinoxylanase |
| 2.378 | 50μg/g | 50μg/g | 200μg/g | 50μg/g | 50μg/g | 100μg/g |
| 1 | 100μg/g | 100μg/g | 250μg/g | 100μg/g | 100μg/g | 150μg/g |
| 0 | 150μg/g | 150μg/g | 300μg/g | 150μg/g | 150μg/g | 200μg/g |
| -1 | 200μg/g | 200μg/g | 350μg/g | 200μg/g | 200μg/g | 250μg/g |
| -2.378 | 250μg/g | 250μg/g | 400μg/g | 250μg/g | 250μg/g | 300μg/g |
Protein and carbohydrate ingredient of the present invention by parsing duck diet, foundation protein and carbohydrate group
Point, the type and dosage of protease and non-starch polysaccharide enzyme are set, is chosen close to the in-vitro simulated of the practical physiological status of animal
Parameter simulates diet digestion process in duck body using regression rotation Orthogonal Composite Design and uses the external effective energy value table of feed
Levy the function and effect of exogenous enzymes, the optimal zymogram added in quick obtaining duck diet.
It should be noted that those skilled in the art are that this hair may be implemented completely according to the various embodiments described above of the present invention
Bright independent claims and the full scope of appurtenance, realize process and the same the various embodiments described above of method；And the present invention is not
It elaborates and partly belongs to techniques well known.
The above, part specific embodiment only of the present invention, but scope of protection of the present invention is not limited thereto, appoints
In the technical scope disclosed by the present invention, any changes or substitutions that can be easily thought of, should all cover by what those skilled in the art
Within protection scope of the present invention.
Claims (1)
1. a kind of duck diet external source zymogram optimization method, includes the following steps: the solution of diet protein and carbohydrate ingredient
Analysis, the setting of exogenous enzyme preparations type and dosage, diet simulation peptic digest, diet simulate intestinal digestion, the optimization of external source zymogram,
It is characterized in that:
(1) parsing of diet protein and carbohydrate ingredient: selection duck diet, which is put into pulverizer, to be crushed 3~5 minutes；It will
Smashed feed crosses 40~60 meshes；- 18~-20 DEG C are placed in store for future use；125~200 milligrams of duck diet are weighed, duck is measured
The content of diet protein and carbohydrate ingredient；
(2) setting of exogenous enzyme preparations type and dosage: according to the duck diet protein and carbohydrate ingredient, according to
Principal component analysis is as a result, weigh 1.8~2.2 grams of duck diet, and add 1~2 kind of protease and 3~5 in the duck diet
0,50,100,150,200,250,300,350,400 μ g/g systems are arranged in the additive capacity of kind carbohydrase, every kind of enzyme preparation
Column concentration gradient is prepared and is added to the enzyme diet of various concentration；
(3) it simulates peptic digest: the sample of the enzyme diet is placed in triangular flask, and simulation peptic digest is added in triangular flask
Buffer, the simulation peptic digest buffer are pH 2.0~3.0, and molar concentration rate is (16~17): the chlorination of 10:10
Sodium: potassium chloride: hydrochloric acid solution；700~800U/mL stomach cardia enzyme solution is added simultaneously；Wherein enzyme diet, simulation peptic digest buffering
Liquid stomach function regulating liquid of protease is according to (2~4): 20:1 weight ratio is uniformly mixed；It will disappear equipped with uniformly mixed enzyme diet, simulation stomach
Change buffer stomach function regulating liquid of protease triangular flask be placed in water bath with thermostatic control shaking table, set water bath with thermostatic control shaking table revolving speed 170~
190rpm and 41~43 DEG C of temperature carry out 4~6h and simulate peptic digest；
(4) it simulates intestinal digestion: after to the simulation peptic digest, sodium chloride is added in the triangular flask: potassium chloride: phosphoric acid
The simulation intestinal digestion buffer of salt, wherein sodium chloride: potassium chloride: phosphatic simulation intestinal digestion buffer molar concentration rate is
(13~18): 3:40；PH 6.2~6.6, unit enzyme activity ratio (85~90): 40:300:30:5:1=tryptose are added simultaneously
Enzyme: chymotrypsin: amylase: maltose: invertase: the simulation intestinal digestion enzyme solution of lipase；Wherein simulation intestinal digestion buffering
The weight percentage ranges of liquid and simulation intestinal digestion enzyme solution are (55~60): 1；Continue the revolving speed 170 of setting water bath with thermostatic control shaking table
~190rpm and 41~43 DEG C of temperature carry out the digestion of 12~16h simulation intestines；
(5) optimization of external source zymogram: after simulating digestion, by slaking residue, drying to constant weight, and measurement residue is effective in vitro
Can value, calculate the external effective energy value of diet of addition every kind of exogenous protease and carbohydrase, and according to exogenous enzymes and
The regression relation of external effective energy value, show that every kind of exogenous enzymes can improve the critical point of the external effective energy value of diet of target；It will
When every kind of exogenous enzymes are encoded according to the critical point, according to the type of a variety of exogenous enzymes of addition, corresponding number is set
5 levels are arranged in impact factor, each impact factor, analyze external effective energy value using regression rotation Orthogonal Composite Design, and
Using external effective energy value and exogenous enzymes additive capacity relationship, optimal external source zymogram is symbolized.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811230125.8A CN109385461A (en) | 2018-10-22 | 2018-10-22 | A kind of duck diet external source zymogram optimization method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811230125.8A CN109385461A (en) | 2018-10-22 | 2018-10-22 | A kind of duck diet external source zymogram optimization method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109385461A true CN109385461A (en) | 2019-02-26 |
Family
ID=65427849
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811230125.8A Pending CN109385461A (en) | 2018-10-22 | 2018-10-22 | A kind of duck diet external source zymogram optimization method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109385461A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110927154A (en) * | 2019-12-13 | 2020-03-27 | 武汉新华扬生物股份有限公司 | Method for evaluating action effect of thermotolerant phytase in vitro |
| CN114657230A (en) * | 2022-02-23 | 2022-06-24 | 中国农业科学院北京畜牧兽医研究所 | Method for evaluating fermentation characteristics of fiber raw material by combining bionic digestion with in-vitro fermentation |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102242180A (en) * | 2011-03-22 | 2011-11-16 | 北京华美源生物科技有限公司 | Optimal enzyme preparation application system (OEATM) |
| CN102590017A (en) * | 2012-02-22 | 2012-07-18 | 北京农学院 | Biomimetic enzyme method for evaluating digestible energy of pig feed |
| CN103882094A (en) * | 2014-01-15 | 2014-06-25 | 中国农业科学院北京畜牧兽医研究所 | Method for screening exogenous carbohydrase spectrum of chicken feed |
| CN105087754A (en) * | 2015-08-06 | 2015-11-25 | 中国农业科学院北京畜牧兽医研究所 | Fast screening method of pig feed non-starch polysaccharide enzyme |
| CN106124429A (en) * | 2016-06-16 | 2016-11-16 | 湖南中本智能科技发展有限公司 | A kind of Bionic digestion assay method of feedstuff digestible carbohydrate total amount |
-
2018
- 2018-10-22 CN CN201811230125.8A patent/CN109385461A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102242180A (en) * | 2011-03-22 | 2011-11-16 | 北京华美源生物科技有限公司 | Optimal enzyme preparation application system (OEATM) |
| CN102590017A (en) * | 2012-02-22 | 2012-07-18 | 北京农学院 | Biomimetic enzyme method for evaluating digestible energy of pig feed |
| CN103882094A (en) * | 2014-01-15 | 2014-06-25 | 中国农业科学院北京畜牧兽医研究所 | Method for screening exogenous carbohydrase spectrum of chicken feed |
| CN105087754A (en) * | 2015-08-06 | 2015-11-25 | 中国农业科学院北京畜牧兽医研究所 | Fast screening method of pig feed non-starch polysaccharide enzyme |
| CN106124429A (en) * | 2016-06-16 | 2016-11-16 | 湖南中本智能科技发展有限公司 | A kind of Bionic digestion assay method of feedstuff digestible carbohydrate total amount |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110927154A (en) * | 2019-12-13 | 2020-03-27 | 武汉新华扬生物股份有限公司 | Method for evaluating action effect of thermotolerant phytase in vitro |
| CN114657230A (en) * | 2022-02-23 | 2022-06-24 | 中国农业科学院北京畜牧兽医研究所 | Method for evaluating fermentation characteristics of fiber raw material by combining bionic digestion with in-vitro fermentation |
| CN114657230B (en) * | 2022-02-23 | 2023-11-24 | 中国农业科学院北京畜牧兽医研究所 | Method for evaluating fermentation characteristics of fiber raw materials by combining bionic digestion and in-vitro fermentation |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Liu et al. | Effect of diet containing phytate and phytase on the activity and messenger ribonucleic acid expression of carbohydrase and transporter in chickens | |
| Al-Rabadi et al. | Effect of extrusion temperature and pre-extrusion particle size on starch digestion kinetics in barley and sorghum grain extrudates | |
| Żyła et al. | Simultaneous application of phytase and xylanase to broiler feeds based on wheat: in vitro measurements of phosphorus and pentose release from wheats and wheat‐based feeds | |
| CN102711513A (en) | Compositions including pyruvate for companion animals and methods of use thereof | |
| CN105146067A (en) | New technology for converting crop straws into pig and chicken forage resource | |
| Lee et al. | Exogenous phytase and xylanase exhibit opposing effects on real-time gizzard pH in broiler chickens | |
| CN109596837A (en) | The Bionic digestion measuring method of one boar food protein digestibility | |
| Traksele et al. | Investigation of in vitro and in vivo digestibility of black soldier fly (Hermetia illucens L.) larvae protein | |
| CN109385461A (en) | A kind of duck diet external source zymogram optimization method | |
| Saki et al. | Intestinal characteristics, alkaline phosphatase and broilers performance in response to extracted and mechanical soybean meal replaced by fish meal | |
| CN108967832A (en) | A kind of detoxicated dose of field corn zeranol and preparation method thereof | |
| Morgan et al. | Non-starch polysaccharide degradation in the gastrointestinal tract of broiler chickens fed commercial-type diets supplemented with either a single dose of xylanase, a double dose of xylanase, or a cocktail of non-starch polysaccharide-degrading enzymes | |
| Perera et al. | Barley, an undervalued cereal for poultry diets: Limitations and opportunities | |
| Lee et al. | Starch characteristics and their influences on in vitro and pig prececal starch digestion | |
| Wang et al. | Effect of probiotic Lactobacillus reuteri XC1 coexpressing endoglucanase and phytase on intestinal pH and morphology, carcass characteristics, meat quality, and serum biochemical indexes of broiler chickens | |
| Osman et al. | Nutrient composition and in vitro fermentation characteristics of sorghum depending on variety and year of cultivation in northern Italy | |
| CN105087754B (en) | The rapid screening method of one boar food non-starch polysaccharide enzyme spectrum | |
| CN104171406A (en) | Selenium-enriched traditional Chinese herbal medicine fermented broiler feed | |
| CN104755616A (en) | Xylanases for solubilising arabinoxylan-containing material | |
| CN110093333A (en) | A kind of application in compound protease and its low-protein diet | |
| Tawfik et al. | Orange pulp in Nile tilapia (Oreochromis niloticus) diets: Growth performance, biochemical parameters and gene expression for growth and fat metabolism | |
| Xu et al. | Effects of different dietary starch sources on growth and glucose metabolism of geese | |
| Sonko et al. | Determination of the biological value of minced semi-finished products with a complex food additive by the enzymatic method. | |
| Zhong et al. | Effects of newly harvested corn on growth performance, intestine development and metabolism of nutrients in broilers | |
| CN109156689A (en) | A kind of albumen generation meal solid beverage and its preparation process |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20190226 |
|
| WD01 | Invention patent application deemed withdrawn after publication |