CN109384856A - A kind of preparation method of fern amylose ultramicron - Google Patents
A kind of preparation method of fern amylose ultramicron Download PDFInfo
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Abstract
The present invention provides a kind of ultramicron preparation methods of fern amylose, and the method comprising the steps of；1) fern fiber crops are pulverized and sieved；2) Soxhlet extractor reflux degreasing；3) it is extracted using water, buck or sour water, centrifugation obtains extracting solution；4) starch is taken off using amylase；5) add dehydrated alcohol to the extracting solution that gained takes off starch, sediment is precipitated, obtains Thick many candies；6) Thick many candies are soluble in water, configure polysaccharide solution；7) dispersant solution is configured；8) polysaccharide solution of equivalent is added into dispersant solution；9) micellization liquid is prepared in high-shear emulsion machine or the processing of high-pressure emulsification machine.The present invention mentioned by Different Extraction Method alkali carries/acid/and water raises solid Thick many candies, it handles without further purification, method is simple, ensure that the various components of polysaccharide；Average particle size is up to 500nm hereinafter, the ultramicron has good stability in the solution in solution, and more conducively intracorporal absorption, bioavilability is high.
Description
Technical field
The present invention relates to a kind of oxidation resistant fern amylose ultramicron preparation methods.
Background technique
Fern fiber crops (Potentilla anserine It L. is) root of rosaceae potentilla plants potentilla anserina, You Mingren
Join fruit, lengthen the life fruit and prickly-pear cactus etc..It is distributed widely in the provinces and regions such as China Jilin, Qinghai, Liaoning, Tibet, Ningxia.All herbal medicine,
With tonifying speen and tonifying kidney, promote the production of body fluid to quench thirst, blood-enrich and other effects.Fern fiber crops are with a long history as Tibetan medicine, but people are more for fern fiber crops
The research of sugar is still shallow.Modern pharmacological studies have shown that fern amylose has the weight-reducing of apparent reducing blood sugar and blood lipid, improves immunity of organism
Power, it is antifatigue, resist oxygen lack and antidiarrheal are antibacterial the effects of, be a kind of rising biological regulator, polysaccharide component is complicated,
The polysaccharide component that Different Extraction Method obtains may be different, and effect is also not quite similar, at present the extraction of fern amylose and effect side
There are reports for the research in face.
The method of the usually used extraction fern amylose of people is to extract fern amylose with water and/or ethyl alcohol at present.Patent
CN1872883A discloses a kind of preparation method of anti-radiation fern amylose, extracts fern amylose using water and ethyl alcohol, passes through
Dialysis bag retention molecular weight purifies polysaccharide, obtains the pure fern amylose of solid, which can enhance the anti-radiation of animal
Ability；Patent CN101619108A discloses a kind of preparation method of ultrasonic wave extraction radiation resistant fern amylose, using ultrasonic wave
Auxiliary water and ethyl alcohol extract fern amylose, same to obtain the pure fern amylose of solid using dialysis purification, can be used for treating X-ray and draw
The radiation injury risen；Patent CN104892784A provides a kind of method of purification of fern amylose, after fern amylose is extracted with water
It is purified using ultrafiltration means, gained fern amylose has antioxidant activity.
In the above patented technology, what the extraction of fern amylose was all made of is the method that traditional water/ethyl alcohol extracts polysaccharide, acid
The method that extraction and alkali carries take fern amylose yet there are no report, its yield of polysaccharide and effect that means of different is extracted may not
Together, we provide a kind of methods for extracting to obtain fern amylose crude product by acid/base in the present invention；
What is be provided which in the above patented technology is the solid powder of fern amylose purifying, and solid polysaccharide is in purification process, retention
Molecule causes the certain active principles of polysaccharide to be lost, and each active principle of polysaccharide is not fully utilized, and microwave and ultrasonic wave extraction can
Polysaccharide molecule structure can be destroyed, the bioactivity of polysaccharide is influenced, it is therefore contemplated that compared with previous product, it can be with pharmaceuticals, guarantor
The mode of health food or functional food efficiently uses the various components of polysaccharide.The report of this aspect is few at present, patent
A kind of preparation of fern leprosy function of odor solid beverage is provided in CN104856182A, using ultrasonic extraction fern fiber crops Thick many candies,
It is mixed mixing with fruit powder, starch to be prepared, having document, (Jin Hui, Zhang Zeming, Zhang Yongjun wait simulated gastric fluid to act on
The in-vitro simulated research " food science and technology " of squash polyoses, 2012,27(4), 178-181.) and report solid polysaccharide or containing polysaccharide
Solid beverage, which takes orally a part of polysaccharide in gastric acid, to be decomposed into monosaccharide by gastric acid, and the oral availability of polysaccharide is caused to drop
It is low.Phosphatide is dispersed in water the near-spherical that can be formed, a part of water phase of encapsulating vesicle, the similar biology of structure
Film has the characteristics that improve encapsulated medicine stability, long-term effect, reduces dosage and be widely used in pharmaceutical carrier.Specially
A kind of preparation method of immunopotentiator is disclosed in benefit 02808956.1, is mentioned through ultra micron after emulsifying polyoses extract
Change to average grain diameter 10um hereinafter, the absorbability of its mucous membrane can be increased, improves the bioavilability of polysaccharide.The present invention provides
A kind of preparation method of stable fern amylose oral administration solution, in such a way that lecithin and polysaccharide are included or are complexed in the solution
The activity of fern amylose in vivo is improved, the various components of polysaccharide is efficiently used, plays anti-oxidant immunological enhancement, the present invention
Middle average particle size is up to 500nm hereinafter, be more advantageous to the absorption of mucous membrane.
Summary of the invention
In view of the shortcomings of the prior art, the purpose of the present invention is to provide a kind of preparations of fern amylose ultramicron solution
Method, which is characterized in that the method includes the steps:
1) fern fiber crops cleaned, drying, crush, sieving, obtain fern fiber crops powder；
2) degreasing: taking the fern fiber crops powder in step 1) to be wrapped in filter paper bucket, be put into Soxhlet extractor, and petroleum ether is added and returns for the first time
Stream；After filter paper packet is dried, second of ethyl alcohol is added into Soxhlet extractor and flows back, volatilizes solvent;
3) it extracts: the obtained fern fiber crops powder of step 2 being put into water, buck or sour water and is extracted, centrifugation obtains extracting solution,
Residue repeats to extract primary；
4) it takes off starch: alpha amylase is added in the obtained extracting solution of step 3), heat lower stirring enzymatic hydrolysis, obtain mentioning for de- starch
Take liquid;
5) alcohol precipitation obtains Thick many candies: precipitating, obtains thick by dehydrated alcohol is added after the obtained extracting solution concentration of step 4)
Polysaccharide；
6) Thick many candies are soluble in water；
7) dispersant solution is configured；
8) polysaccharide solution of equivalent is added into dispersant solution, and stirs evenly；
9) micellization liquid is prepared in high-shear emulsion machine or the processing of high-pressure emulsification machine.
Wherein, the buck in step 3) is sodium hydrate aqueous solution, and preferred concentration is 0.3 ~ 0.7mol/L.
Wherein, sour water is aqueous hydrochloric acid solution in step 3), and preferred concentration is 0.2 ~ 0.6mol/L.
Wherein, extraction described in step 3) is 2 ~ 4h of stirring under the conditions of 40 ~ 80 DEG C, and then adjusting pH is neutrality.
Wherein, centrifugal rotational speed described in step 3) is 7000 ~ 10000 revs/min, and the time is 2 ~ 5 minutes.
Wherein, the first time return time in step 2 is 2 ~ 4h, and second of return time is 2 ~ 4h.
Wherein, concentration of alcohol is 70 ~ 95% in step 2.
Wherein, alpha amylase is the 0.05 ~ 0.1% of fern fiber crops powder weight in step 4).
Wherein, heating temperature is 50 ~ 70 DEG C in step 4), and enzymolysis time is 1 ~ 5h.
Wherein, dehydrated alcohol is added to the final concentration of 50% ~ 90%(v/v of ethyl alcohol) in step 5).
Wherein, the mass fraction of polysaccharide solution is 1%-5% in step 6).
Wherein, dispersing agent is surfactant, preferably lecithin in step 7).
Wherein, the mass fraction of dispersing agent is 2%-10% in step 7).
Wherein, the height in step 9) cuts the revolving speed 5000rad/min or more of mulser.
Wherein, the high-pressure emulsification machine emulsification pressure in step 9) is 300kgf/cm2More than.
Wherein, the micellization liquid average grain diameter 500nm or less in step 9).
Wherein the enzyme activity of alpha amylase described in step 4) is no less than 2000U/g.
Compared with prior art, the beneficial effects of the present invention are: mentioned by Different Extraction Method alkali carries/acid/water raises
Solid Thick many candies, are handled without further purification, and method is simple, ensure that the various components of polysaccharide；In the solution by polysaccharide and lecithin
Inclusion/complexing is prepared for stable fern amylose ultramicron oral administration solution, it is ensured that the activity of polysaccharide in vivo, the results showed that
In solution average particle size up to 500nm hereinafter, the ultramicron has good stability in the solution, more conducively intracorporal suction
It receives, bioavilability is high.
Detailed description of the invention
Fig. 1: micellization fern amylose extract particles size distribution figure in embodiment 1；Horizontal axis indicates partial size (nm), the longitudinal axis
The frequency (%) of particle is indicated with bar graph.
Fig. 2: micellization fern amylose extract particles size distribution figure in embodiment 2；Horizontal axis indicates partial size (nm), the longitudinal axis
The frequency (%) of particle is indicated with bar graph.
Fig. 3: micellization fern amylose extract particles size distribution figure in embodiment 3；Horizontal axis indicates partial size (nm), the longitudinal axis
The frequency (%) of particle is indicated with bar graph.
Fig. 4: micellization fern amylose extract particles size distribution figure in embodiment 4；Horizontal axis indicates partial size (nm), the longitudinal axis
The frequency (%) of particle is indicated with bar graph.
Fig. 5: micellization fern amylose extract particles size distribution figure in embodiment 5；Horizontal axis indicates partial size (nm), the longitudinal axis
The frequency (%) of particle is indicated with bar graph.
Fig. 6: micellization fern amylose extract particles size distribution figure in embodiment 6；Horizontal axis indicates partial size (nm), the longitudinal axis
The frequency (%) of particle is indicated with bar graph.
Fig. 7: micellization fern amylose extract particles size distribution figure in embodiment 7；Horizontal axis indicates partial size (nm), the longitudinal axis
The frequency (%) of particle is indicated with bar graph.
Specific embodiment
The present invention is specifically described below by example, it is necessary to which indicated herein is that following instance is only intended to pair
The present invention is further described, and should not be understood as limiting the scope of the invention, person skilled in the art's root in the field
The some nonessential modifications and adaptations made according to foregoing invention content, still fall within protection scope of the present invention.
Embodiment 1
1) fern fiber crops cleaned, drying, crush, cross 40 meshes；
2) degreasing: taking the fern fiber crops powder in step 1) to be wrapped in filter paper bucket, be put into Soxhlet extractor, and petroleum ether is added, and flow back 2 h
To slough surface fat；After filter paper packet is dried, 85% ethyl alcohol is added into Soxhlet extractor, the 2h that flows back is to remove small point of part
Son and pigment, volatilize solvent, spare；
3) it extracts: the sodium hydrate aqueous solution that the concentration that addition volume is 20 times of volume shared by fern fiber crops dry weight is 0.5mol/L,
2h is stirred under the conditions of 60 DEG C, adjusting pH is neutrality, under conditions of revolving speed is 10000 revs/min, is centrifuged 5 minutes, and residue repeats to extract
Once, extracting solution is obtained；
4) it takes off starch: the obtained extracting solution of step 3) being added to 0.1% alpha amylase of fern fiber crops powder weight, is stirred at 65 DEG C
4h is digested, inactivation obtains the extracting solution of de- starch；
5) alcohol precipitation obtains Thick many candies: by middle addition dehydrated alcohol after the obtained de- starch solution concentration of step 4), until ethyl alcohol is whole
Concentration is 75%(v/v), it is precipitated, obtains Thick many candies, recovery rate 25.06%；
6) Thick many candies add distilled water stirring and dissolving, are configured to the polysaccharide solution that mass fraction is 1%；
7) lecithin soln that configuration quality score is 2%；
8) polysaccharide solution of quality such as it is added into lecithin soln, 2h is to uniform for stirring；
9) high-shear emulsion machine is stirred at room temperature 4h (revolving speed 6000rad/min) and is prepared micellization liquid, nanometer dynamics and
It is 136.2nm that Zeta potential analyzer, which measures micella average particle size,.
Embodiment 2
1) fern fiber crops cleaned, drying, crush, cross 60 meshes；
2) degreasing: taking the fern fiber crops powder in step 1) to be wrapped in filter paper bucket, be put into Soxhlet extractor, and petroleum ether is added, and flow back 2 h
To slough surface fat；After filter paper packet is dried, 90% ethyl alcohol is added into Soxhlet extractor, the 2h that flows back is to remove small point of part
Son and pigment, volatilize solvent, spare；
3) it extracts: the sodium hydrate aqueous solution that the concentration that addition volume is 20 times of volume shared by fern fiber crops dry weight is 0.6mol/L,
2h is stirred under the conditions of 70 DEG C, adjusting pH is neutrality, under conditions of revolving speed is 10000 revs/min, is centrifuged 5 minutes, and residue repeats to extract
Once, extracting solution is obtained；
4) it takes off starch: the obtained extracting solution of step 3) being added to 0.05% alpha amylase of fern fiber crops powder weight, is stirred at 65 DEG C
Enzymatic hydrolysis 4h is mixed, inactivation obtains the extracting solution of de- starch；
5) alcohol precipitation obtains Thick many candies: by middle addition dehydrated alcohol after the obtained de- starch solution concentration of step 4), until ethyl alcohol is whole
Concentration is 50%(v/v), it is precipitated, obtains Thick many candies, recovery rate 23.74%；
6) Thick many candies add distilled water stirring and dissolving, are configured to the polysaccharide solution that mass fraction is 2%；
7) lecithin soln that configuration quality score is 5%；
8) polysaccharide solution of quality such as it is added into lecithin soln, 2h is to uniform for stirring；
9) high-shear emulsion machine is stirred at room temperature 4h (revolving speed 5000rad/min) and micellization liquid, nanometer dynamics and Zeta is prepared
It is 492.8nm that potential measurement instrument, which measures micella average particle size,.
Embodiment 3
1) fern fiber crops cleaned, drying, crush, cross 80 meshes；
2) degreasing: taking the fern fiber crops powder in step 1) to be wrapped in filter paper bucket, be put into Soxhlet extractor, and petroleum ether is added, and flow back 2 h
To slough surface fat；After filter paper packet is dried, 95% ethyl alcohol is added into Soxhlet extractor, the 2h that flows back is to remove small point of part
Son and pigment, volatilize solvent, spare；
3) it extracts: the sodium hydrate aqueous solution that the concentration that addition volume is 20 times of volume shared by fern fiber crops dry weight is 0.7mol/L,
2h is stirred under the conditions of 70 DEG C, adjusting pH is neutrality, under conditions of revolving speed is 10000 revs/min, is centrifuged 5 minutes, and residue repeats to extract
Once, extracting solution is obtained；
4) it takes off starch: the obtained extracting solution of step 3) being added to 0.1% alpha amylase of fern fiber crops powder weight, is stirred at 65 DEG C
4h is digested, inactivation obtains the extracting solution of de- starch；
5) alcohol precipitation obtains Thick many candies: by middle addition dehydrated alcohol after the obtained de- starch solution concentration of step 4), until ethyl alcohol is whole
Concentration is 60%(v/v), it is precipitated, obtains Thick many candies, recovery rate 24.54%；
6) Thick many candies add distilled water stirring and dissolving, are configured to the polysaccharide solution that mass fraction is 3%；
7) lecithin soln that configuration quality score is 7%；
8) polysaccharide solution of quality such as it is added into lecithin soln, 2h is to uniform for stirring；
9) high-shear emulsion machine is stirred at room temperature 4h (revolving speed 6500rad/min) and micellization liquid, nanometer dynamics and Zeta is prepared
It is 59.5nm that potential measurement instrument, which measures micella average particle size,.
Embodiment 4
1) fern fiber crops cleaned, drying, crush, cross 80 meshes；
2) degreasing: taking the fern fiber crops powder in step 1) to be wrapped in filter paper bucket, be put into Soxhlet extractor, and petroleum ether is added, and flow back 2 h
To slough surface fat；After filter paper packet is dried, 95% ethyl alcohol is added into Soxhlet extractor, the 2h that flows back is to remove small point of part
Son and pigment, volatilize solvent, spare；
3) it extracts: the aqueous hydrochloric acid solution that the concentration that addition volume is 20 times of volume shared by fern fiber crops dry weight is 0.3mol/L, at 80 DEG C
Under the conditions of stir 2h, adjusting pH is neutrality, under conditions of revolving speed is 10000 revs/min, is centrifuged 5 minutes, and residue repeats to extract one
It is secondary, obtain extracting solution；
4) it takes off starch: the obtained extracting solution of step 3) being added to 0.1% alpha amylase of fern fiber crops powder weight, is stirred at 65 DEG C
4h is digested, inactivation obtains the extracting solution of de- starch；
5) alcohol precipitation obtains Thick many candies: by middle addition dehydrated alcohol after the obtained de- starch solution concentration of step 4), until ethyl alcohol is whole
Concentration is 60%(v/v), it is precipitated, obtains Thick many candies, recovery rate 26.88%；
6) Thick many candies add distilled water stirring and dissolving, are configured to the polysaccharide solution that mass fraction is 2.5%；
7) lecithin soln that configuration quality score is 5%；
8) polysaccharide solution of quality such as it is added into lecithin soln, 2h is to uniform for stirring；
9) high-shear emulsion machine is stirred at room temperature 4h (revolving speed 6500rad/min) and micellization liquid, nanometer dynamics and Zeta is prepared
It is 62.6nm that potential measurement instrument, which measures micella average particle size,.
Embodiment 5
1) fern fiber crops cleaned, drying, crush, cross 60 meshes；
2) degreasing: taking the fern fiber crops powder in step 1) to be wrapped in filter paper bucket, be put into Soxhlet extractor, and petroleum ether is added, and flow back 2 h
To slough surface fat；After filter paper packet is dried, 90% ethyl alcohol is added into Soxhlet extractor, the 2h that flows back is to remove small point of part
Son and pigment, volatilize solvent, spare；
3) it extracts: the aqueous hydrochloric acid solution that the concentration that addition volume is 30 times of volume shared by fern fiber crops dry weight is 0.4mol/L, at 70 DEG C
Under the conditions of stir 2h, adjusting pH is neutrality, under conditions of revolving speed is 10000 revs/min, is centrifuged 5 minutes, and residue repeats to extract one
It is secondary, obtain extracting solution；
4) it takes off starch: the obtained extracting solution of step 3) being added to 0.05% alpha amylase of fern fiber crops powder weight, is stirred at 60 DEG C
Enzymatic hydrolysis 4h is mixed, inactivation obtains the extracting solution of de- starch；
5) alcohol precipitation obtains Thick many candies: by middle addition dehydrated alcohol after the obtained de- starch solution concentration of step 4), until ethyl alcohol is whole
Concentration is 50%(v/v), it is precipitated, obtains Thick many candies, recovery rate 27.88%；
6) Thick many candies add distilled water stirring and dissolving, are configured to the polysaccharide solution that mass fraction is 5%；
7) lecithin soln that configuration quality score is 10%；
8) polysaccharide solution of quality such as it is added into lecithin soln, 2h is to uniform for stirring；
9) high-shear emulsion machine is stirred at room temperature 4h (revolving speed 6300rad/min) and micellization liquid, nanometer dynamics and Zeta is prepared
It is 147.4nm that potential measurement instrument, which measures micella average particle size,.
Embodiment 6
1) fern fiber crops cleaned, drying, crush, cross 40 meshes；
2) degreasing: taking the fern fiber crops powder in step 1) to be wrapped in filter paper bucket, be put into Soxhlet extractor, and petroleum ether is added, and flow back 3 h
To slough surface fat；After filter paper packet is dried, 85% ethyl alcohol is added into Soxhlet extractor, the 3h that flows back is to remove small point of part
Son and pigment, volatilize solvent, spare；
3) it extracts: the aqueous hydrochloric acid solution that the concentration that addition volume is 20 times of volume shared by fern fiber crops dry weight is 0.2mol/L, at 60 DEG C
Under the conditions of stir 2h, adjusting pH is neutrality, under conditions of revolving speed is 10000 revs/min, is centrifuged 5 minutes, and residue repeats to extract one
It is secondary, obtain extracting solution；
4) it takes off starch: the obtained extracting solution of step 3) being added to 0.1% alpha amylase of fern fiber crops powder weight, is stirred at 65 DEG C
4h is digested, inactivation obtains the extracting solution of de- starch；
5) alcohol precipitation obtains Thick many candies: by middle addition dehydrated alcohol after the obtained de- starch solution concentration of step 4), until ethyl alcohol is whole
Concentration is 75%(v/v), it is precipitated, obtains Thick many candies, recovery rate 22.85%；
6) Thick many candies add distilled water stirring and dissolving, are configured to the polysaccharide solution that mass fraction is 4%；
7) lecithin soln that configuration quality score is 7.5%；
8) polysaccharide solution of quality such as it is added into lecithin soln, 2h is to uniform for stirring；
9) high-shear emulsion machine is stirred at room temperature 4h (revolving speed 7000rad/min) and micellization liquid, nanometer dynamics and Zeta is prepared
It is 47.3nm that potential measurement instrument, which measures micella average particle size,.
Embodiment 7
1) fern fiber crops cleaned, drying, crush, cross 40 meshes；
2) degreasing: taking the fern fiber crops powder in step 1) to be wrapped in filter paper bucket, be put into Soxhlet extractor, and petroleum ether is added, and flow back 3 h
To slough surface fat；After filter paper packet is dried, 85% ethyl alcohol is added into Soxhlet extractor, the 3h that flows back is to remove small point of part
Son and pigment, volatilize solvent, spare；
3) it extracts: the distilled water solution that volume is 20 times of volume shared by fern fiber crops dry weight is added, stirs 2h under the conditions of 90 DEG C, turning
It under conditions of speed is 10000 revs/min, is centrifuged 5 minutes, residue repetition extraction is primary, obtains extracting solution；
4) it takes off starch: the obtained extracting solution of step 3) being added to 0.1% alpha amylase of fern fiber crops powder weight, is stirred at 65 DEG C
4h is digested, inactivation obtains the extracting solution of de- starch；
5) alcohol precipitation obtains Thick many candies: by middle addition dehydrated alcohol after the obtained de- starch solution concentration of step 4), until ethyl alcohol is whole
Concentration is 75%(v/v), it is precipitated, obtains Thick many candies, recovery rate 9.07%；
6) Thick many candies add distilled water stirring and dissolving, are configured to the polysaccharide solution that mass fraction is 2%；
7) lecithin soln that configuration quality score is 6%；
8) polysaccharide solution of quality such as it is added into lecithin soln, 2h is to uniform for stirring；
9) high-shear emulsion machine is stirred at room temperature 4h (revolving speed 6400rad/min) processing and is prepared micellization liquid, nanometer dynamics and
It is 128.0nm that Zeta potential analyzer, which measures micella average particle size,.
Claims (10)
1. a kind of fern amylose ultramicron preparation method, which is characterized in that the described method comprises the following steps；
1) fern fiber crops cleaned, drying, crush, sieving, obtain fern fiber crops powder；
2) degreasing: taking the fern fiber crops powder in step 1) to be wrapped in filter paper bucket, be put into Soxhlet extractor, and petroleum ether is added and returns for the first time
Stream；After filter paper packet is dried, second of reflux of ethyl alcohol is added into Soxhlet extractor and volatilizes solvent；
3) it extracts: the obtained fern fiber crops powder of step 2 being put into water, buck or sour water and is extracted, centrifugation obtains extracting solution,
Residue repeats to extract primary；
4) it takes off starch: alpha amylase is added in the obtained extracting solution of step 3), heat lower stirring enzymatic hydrolysis, obtain mentioning for de- starch
Take liquid；
5) alcohol precipitation obtains Thick many candies: middle addition dehydrated alcohol after the obtained de- starch solution concentration of step 4) is precipitated,
Obtain Thick many candies；
6) Thick many candies are soluble in water；
7) dispersant solution is configured；
8) polysaccharide solution of equivalent is added into dispersant solution, and stirs evenly；
9) micellization liquid is prepared in high-shear emulsion machine or the processing of high-pressure emulsification machine.
2. preparation method as described in claim 1, it is characterised in that the buck in the step 3) is sodium hydrate aqueous solution,
The sodium hydrate aqueous solution concentration is 0.3 ~ 0.7mol/L, and sour water is aqueous hydrochloric acid solution, and concentration is 0.2 ~ 0.6mol/L.
3. preparation method as described in claim 1, it is characterised in that extraction described in the step 3) is in 40 ~ 80 DEG C of conditions
2 ~ 4h of lower stirring, then adjusting pH is neutrality.
4. preparation method as described in claim 1, it is characterised in that centrifugal rotational speed described in the step 3) be 7000 ~
10000 revs/min, the time is 2 ~ 5 minutes.
5. preparation method as described in claim 1, it is characterised in that the first time return time in the step 2 is 2 ~ 4h,
Second of return time is 2 ~ 4h.
6. preparation method as described in claim 1, it is characterised in that alpha amylase is fern fiber crops powder weight in the step 4)
0.05 ~ 0.1%, heating temperature is 50 ~ 70 DEG C in step 4), and enzymolysis time is 1 ~ 5h.
7. preparation method as described in claim 1, it is characterised in that dehydrated alcohol is added to ethyl alcohol final concentration in the step 5)
For 50% ~ 90%(v/v).
8. preparation method as described in claim 1, it is characterised in that the mass fraction of polysaccharide solution is 1%- in the step 6)
5%。
9. preparation method as described in claim 1, it is characterised in that dispersing agent is surfactant in the step 7), preferably
Lecithin, the mass fraction of dispersing agent are 2%-10%.
10. preparation method as described in claim 1, it is characterised in that the revolving speed of the high mulser earnestly in the step 9)
5000rad/min or more, high-pressure emulsification machine emulsification pressure is 300kgf/cm2More than, the micellization liquid average grain diameter in step 9)
500nm or less.
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| CN115227670A (en) * | 2022-06-24 | 2022-10-25 | 山东博德医药研究院有限公司 | Method for optimizing burdock polysaccharide nanoparticles |
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