CN109380634A - A kind of stalk functional beverage and preparation method thereof - Google Patents
A kind of stalk functional beverage and preparation method thereof Download PDFInfo
- Publication number
- CN109380634A CN109380634A CN201811472788.0A CN201811472788A CN109380634A CN 109380634 A CN109380634 A CN 109380634A CN 201811472788 A CN201811472788 A CN 201811472788A CN 109380634 A CN109380634 A CN 109380634A
- Authority
- CN
- China
- Prior art keywords
- stalk
- added
- preparation
- filter residue
- functional beverage
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
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- 238000002360 preparation method Methods 0.000 title claims abstract description 29
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- XPFJYKARVSSRHE-UHFFFAOYSA-K trisodium;2-hydroxypropane-1,2,3-tricarboxylate;2-hydroxypropane-1,2,3-tricarboxylic acid Chemical compound [Na+].[Na+].[Na+].OC(=O)CC(O)(C(O)=O)CC(O)=O.[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O XPFJYKARVSSRHE-UHFFFAOYSA-K 0.000 claims description 8
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Mycology (AREA)
- Non-Alcoholic Beverages (AREA)
Abstract
A kind of stalk functional beverage and preparation method thereof, which is characterized in that the preparation method comprises the following steps: Dry corn stalk stalk raw material preparation, water logging, dries pulverizing, alkaline ethanol solution washing, washing, acidic xylan enzyme hydrolysis, laccase hydrolysis, acid cellulose enzyme hydrolysis, high speed dispersion processing, high-pressure homogeneous processing, decoloration washing, flavor mixing processing, sterilization, cooling are filling.The invention has the beneficial effects that: a kind of stalk functional beverage provided by the invention has more health value while as a beverage, healthy and safe, has the characteristics that pure natural, can take for a long time, and convenient to take, with good market prospects；And preparation method have the advantages that low energy consumption, without using chemical hazard reagent, by-product is few, simple, Yi Shixian, the recycling of resource also may be implemented using discarded stalk, alleviate the pollution to environment.
Description
Technical field
The invention belongs to functional beverage preparation fields, and in particular to a kind of stalk functional beverage and preparation method thereof.
Background technique
The ingredient and content ratio that drinks refer to by adjusting nutrient in beverage, adjust human body to a certain extent
The beverage of function.Classification according to relevant information to functional beverage, it is believed that the drinks of broad sense include sports drink, energy drink
Material has the beverage of health-care effect with other.Drinks are that fashionable one kind in developed countries such as American-European and Japan is strong over 2000
Kang Yinpin.It contains the electrolyte such as potassium, sodium, calcium, magnesium, and ingredient is similar to human body fluid, can more be absorbed by the body rapidly after drinking,
The human body moisture and electrolyte (salinity) lost because of a large amount of movement perspirations is replenished in time, body fluid is made to reach equilibrium state.Work as drink
Become a kind of fashion with functional beverage, this industry is also thriving therewith.Trade journal " beverage series " edits Barry
Na Tansong says that the industrial value of functional beverage has been up to 1,500,000,000 dollars, and product type is more than 150 kinds.Drinks are in China
Liked by more and more consumers, China is increasingly becoming the consumption big country of functional beverage.
But current market sales of functional beverage generally all add caffeine energy excitor nerve substance, and this
A little substance long-term consumptions are not conducive to good health, so we need a kind of natural functional beverage, both containing abundant
Nutrient, and be not unfavorable for the substance of human health.
Summary of the invention
To solve the deficiencies in the prior art, the purpose of the present invention is to provide one kind rich in amino acid, minerals, dimension life
Element is beneficial to health, and has health value stalk functional beverage and preparation method thereof.
A kind of preparation method of stalk functional beverage, comprising the following steps:
(1) Dry corn stalk stalk raw material preparation: corn dry straw is cut to size 3cm or so, remove dust, the impurity on surface
Deng to get arrive Dry corn stalk stalk raw material；The too long operation that will affect subsequent step of the length of stalk；
(2) water logging: for 24 hours with distilled water immersion by the ready Dry corn stalk stalk raw material, filter residue is collected by filtration later
Spare, the filter residue is labeled as No. 1 filter residue；Soaking process requires Dry corn stalk stalk raw material described in distillation water submerged, so not only
The impurity such as dust can be removed, additionally it is possible to the Dry corn stalk stalk raw tissue softening be made to be convenient for the operation of subsequent step；
(3) dries pulverizing: No. 1 filter residue being put into thermostatic drying chamber and is dried, is crushed to 80 mesh later,
Obtain stalk powder；
(4) alkaline ethanol solution washs: taking a certain amount of stalk powder to be added in alkaline ethanol solution and slowly heats up
To 80 DEG C, 2h is reacted, filter residue is collected in filtering, is labeled as No. 2 filter residues；
(5) it washes: No. 2 filter residues being soaked in distilled water and maintain for 24 hours, to remove the wood in No. 2 filter residue tissues
Quality substance；
(6) acidic xylan enzyme hydrolysis: the substance taking-up after washing is placed in suitable container, according to solid-liquid ratio 1:20
0.05moL/L citric acid-sodium citrate buffer solution (pH=4.8) 200mL is added in ratio, is put into thermostat water bath and is warming up to 50
~55 DEG C, zytase 0.5g is added later, reaction time 12h obtains acidic xylan protease hydrolysate；
Addition acidic xylanase effectively alleviates the deposited phenomenon in fruit juice, deposited phenomenon be because
Contain more polysaccharose substance in fruit juice；
(7) laccase hydrolyzes: 0.2moL/L acetic acid-is added according to solid-liquid ratio 1:20 in the acidic xylan protease hydrolysate
Sodium acetate buffer (pH=4.8) 200mL, and hydrogen peroxide 0.6mL is added, 50~55 DEG C are warming up to, laccase 0.1g is added later,
Reaction time 3h obtains laccase hydrolysate；
Laccase effectively prevents the oxidizing brown stain of the stalk functional beverage, and makes the color of beverage more clear
Clearly, structural state is more stable；
(8) 0.05moL/L lemon acid cellulose enzyme hydrolysis: is added according to solid-liquid ratio 1:20 in the laccase hydrolysate
Acid-sodium citrate buffer solution (pH=4.8) 200mL, is put into thermostat water bath and is warming up to 50~55 DEG C, cellulose is added later
Enzyme 0.05g, reaction time 5h obtain acid cellulose protease hydrolysate；
Acidic cellulase can effectively soften plant tissue, promote the release of cellular content, improve crushing juice rate；
(9) high speed dispersion is handled: the acid cellulose protease hydrolysate carries out centrifugal treating, collects filter residue, is labeled as No. 3
No. 3 filter residues are carried out high speed dispersion and handle 2min, obtain high speed dispersion suspension by filter residue；
(10) high-pressure homogeneous processing: the high speed dispersion suspension is put into homogenizer, handles 30s, it is equal to obtain high pressure
Matter handles suspension；
(11) decoloration washing: the high-pressure homogeneous processing suspension is filtered, and collects filter residue, is labeled as No. 4 filter residues,
The hydrogen peroxide that concentration is 0.02mol/L is added in No. 4 filter residues, reacts 30min under the conditions of 50 DEG C, washes 5 times later,
Complete the preparation of nano-cellulose fiber NFC；
(12) flavor mixing is handled: a certain amount of sucrose and citric acid are added in the nano-cellulose fiber NFC, and
Appropriate pectin and sodium carboxymethylcellulose are added, beverage first product is obtained；
Pectin is a kind of hydrophilic natural plant gum, and the addition of pectin can not only reduce the generation of precipitating, can also make institute
The structure stability for stating stalk functional beverage is stronger, obtains the mouthfeel for being different from Other Drinks；Sodium carboxymethylcellulose is being eaten
Product application aspect plays the role of increasing toughness, concentration, the stability of food；And pectin and sodium carboxymethylcellulose are all nothings
The food additives of toxic side effect have met production safety, the demand of natural drink；
(13) it sterilizes, is cooling filling: by the way of the sterilization of low temperature bus, water bath with thermostatic control being warming up to 62 DEG C, is put into drink
Expect that first product carries out the sterilization of low temperature bus, sterilizing time 30min is cooled to room temperature filling later.
Bath temperature cannot be excessively high because natural beverage made of fruits or vegetables is food low in calories, it is contained therein also all
It is the component content that some human bodies easily absorb, such as monosaccharide, inorganic salts, vitamin C, high temperature can make nutritional ingredient therein
It is destroyed；The sterilization of low temperature bus can kill pathogenic bacteria and heat labile microorganism, and can guarantee that the stalk is functional
The genuineness of beverage.
The mass ratio of the corn dry straw, sucrose and citric acid is 50:20:1.
The acidic xylan enzyme hydrolysis, laccase hydrolyze, the temperature of acid cellulose enzyme hydrolysis is all 50 DEG C.
A kind of stalk functional beverage is prepared by a kind of preparation method of stalk functional beverage above-mentioned.
The invention has the beneficial effects that: a kind of stalk functional beverage provided by the invention is rich in soluble sugar, amino
Acid, minerals, vitamin, are beneficial to health, while having the function of adsorbing grease, cholesterol, removing nitrite,
While as a beverage, health value is had more, it is healthy and safe, have the characteristics that pure natural, can take for a long time, and take
It is convenient, there are good market prospects；And preparation method compares conventional method with low energy consumption, soda acid usage amount is few, no
Using chemical hazard reagent, the advantages of by-product is few, simple, Yi Shixian, the recycling of resource is realized using discarded stalk,
Maximum cost performance is obtained with the smallest cost, and waste straw recycling reduces crop straw burning amount, alleviates to environment
Pollution.
Specific embodiment
Embodiment 1
A kind of preparation method of stalk functional beverage, comprising the following steps:
(1) Dry corn stalk stalk raw material preparation: 10g corn dry straw being cut to size 3cm or so, remove surface dust,
Impurity etc. to get arrive Dry corn stalk stalk raw material；The too long operation that will affect subsequent step of the length of stalk；
(2) water logging: for 24 hours with distilled water immersion by the ready Dry corn stalk stalk raw material, filter residue is collected by filtration later
Spare, the filter residue is labeled as No. 1 filter residue；Soaking process requires Dry corn stalk stalk raw material described in distillation water submerged, so not only
The impurity such as dust can be removed, additionally it is possible to the Dry corn stalk stalk raw tissue softening be made to be convenient for the operation of subsequent step；
(3) dries pulverizing: No. 1 filter residue being put into thermostatic drying chamber and is dried, is crushed to 80 mesh later,
Obtain stalk powder；
(4) alkaline ethanol solution washs: taking a certain amount of stalk powder to be added in alkaline ethanol solution and slowly heats up
To 80 DEG C, 2h is reacted, filter residue is collected in filtering, is labeled as No. 2 filter residues；
(5) it washes: No. 2 filter residues being soaked in distilled water and maintain for 24 hours, to remove the wood in No. 2 filter residue tissues
Quality substance；
(6) acidic xylan enzyme hydrolysis: the substance taking-up after washing is placed in suitable container, 0.05moL/L is added
Citric acid-sodium citrate buffer solution (pH=4.8) 200mL, is put into thermostat water bath and is warming up to 50 DEG C, xylan is added later
Enzyme 0.5g, reaction time 12h obtain acidic xylan protease hydrolysate；
(7) laccase hydrolyzes: 0.2moL/L acetic acid-sodium acetate buffer solution being added in the acidic xylan protease hydrolysate
(pH=4.8) 200mL, and hydrogen peroxide 0.6mL is added, 50 DEG C are warming up to, laccase 0.1g is added later, reaction time 3h is obtained
Laccase hydrolysate；
(8) it is slow that 0.05moL/L citric acid-sodium citrate acid cellulose enzyme hydrolysis: is added in the laccase hydrolysate
Fliud flushing (pH=4.8) 200mL, is put into thermostat water bath and is warming up to 50 DEG C, and cellulase 0.05g, reaction time are added later
5h obtains acid cellulose protease hydrolysate；
(9) high speed dispersion is handled: the acid cellulose protease hydrolysate carries out centrifugal treating, collects filter residue, is labeled as No. 3
No. 3 filter residues are carried out high speed dispersion and handle 2min, obtain high speed dispersion suspension by filter residue；
(10) high-pressure homogeneous processing: the high speed dispersion suspension is put into homogenizer, handles 30s, it is equal to obtain high pressure
Matter handles suspension；
(11) decoloration washing: the high-pressure homogeneous processing suspension is filtered, and collects filter residue, is labeled as No. 4 filter residues,
The hydrogen peroxide that concentration is 0.02mol/L is added in No. 4 filter residues, reacts 30min under the conditions of 50 DEG C, washes 5 times later
The preparation of nano-cellulose fiber NFC is completed in left and right；
(12) flavor mixing is handled: being added 4g sucrose and 0.2g citric acid in the NFC, and is added appropriate pectin and carboxylic
Sodium carboxymethylcellulose pyce obtains beverage first product；
(13) it sterilizes, is cooling filling: by the way of the sterilization of low temperature bus, water bath with thermostatic control being warming up to 62 DEG C, is put into drink
Expect that first product carries out the sterilization of low temperature bus, sterilizing time 30min is cooled to room temperature filling later.
Properties of product detection:
1. sensory evaluation
Specific evaluation criterion is shown in Table 1.
1 sensory evaluation criteria table of table
According to the standards of grading of table 1, sensory evaluation is carried out to a kind of stalk functional beverage obtained in embodiment 1: outer
See limpid, the impurity such as no precipitating, suspended matter；The natural taste of beverage made of fruits or vegetables is remained, without undesirable smell, sweet mouthfeel is suitable
In, special taste；It is scored at 85.9.
Use commercial product for control group, sensory evaluation is scored at 74.5.
2. nutritional ingredient is evaluated
Experimentation:
(1) measurement of soluble sugar
Using reversed-phased high performace liquid chromatographic to soluble sugar (including glucose, fructose, sucrose, the wood in stalk beverage
Sugar, mannose, arabinose, lactose, galactolipin) it is analyzed, it is functional to weigh a kind of stalk prepared in 5mL embodiment 1
Beverage is diluted with water, and is settled to 10.0mL, shakes up, and crosses 0.22 μm of acetate fiber filter membrane, and micro syringe takes 20 μ L filtrate columns
Enter in chromatograph and is analyzed.
(2) ICP-MS of microelement and heavy metal element is measured
A kind of stalk functional beverage prepared in 0.5mL embodiment 1 is weighed in 50mL small beaker, 25mL is added and boils
Deionized water impregnate 15min, therefrom pipette 5mL and be settled to 50mL, using inductivity coupled plasma mass spectrometry (ICP-MS) method
12 kinds of elements are had altogether comprising Na, Mg, K, Ca, Mn, Zn, Fe, Cu, As, Se, Cd, Pb in measurement stalk functional beverage, are used in combination
Working curve method calculates content or residual quantity.
(3) analysis of amino acid content
Utilize the free amine group in a kind of stalk functional beverage prepared in high performance liquid chromatograph analysis embodiment 1
Acid, sample is after solid-phase extraction column Oasis HLB extraction, then the upper HPLC instrument after 0.22 μm of membrane filtration, using Acclaim
2 chromatographic column of Explosive E (2.1mm × 150mm, 3 μm) separation, using methanol -10mmo L/L ammonium acetate solution as mobile phase,
The amino acid under gradient elution mode.
(4) analysis of Vitamin C content
Using Vitamin C content in iodometric determination beverage.
Experimental result: know that soluble sugar is main in a kind of stalk functional beverage prepared in embodiment 1 by analysis
It is fructose and sucrose two major classes, fructose content highest, maximum level is 5 times of sucrose maximum level, measures to obtain fructose content
For 12.35mg/mL；The content of mineral matter element K, Na, Ca, Mg, Zn, Fe and Mn element be respectively 1567,75.8., 745,
587,2.05,1.25 and 3.14mg/L, heavy metal element Cu, As, Se, Cd, Pb are not detected；16 kinds of amino are detected altogether
Acid, wherein alanine, histidine, serine, lysine, the content of threonine are more, threonine needed by human, valine,
7 kinds of methionine, phenylalanine, isoleucine, leucine, lysine amino acid contents are respectively 1.51,0.48,0.61,0.20,
0.17,0.56 and 0.94mg/100mL；Vitamin C content is relatively high, has reached 71.16 μ g/g.
Use commercially available three kinds of products for control group simultaneously, the experimental results showed that amino acid in the functional beverage of blending type
Type is less, only alanine, two class of glutamic acid, and for carbohydrate also based on sucrose, plant fermented liquid is in basic functional beverage
The type of amino acid is more, but every kind of content is relatively low.
Embodiment 2
A kind of preparation method of stalk functional beverage, comprising the following steps:
(1) Dry corn stalk stalk raw material preparation: 10g corn dry straw being cut to size 3cm or so, remove surface dust,
Impurity etc. to get arrive Dry corn stalk stalk raw material；The too long operation that will affect subsequent step of the length of stalk；
(2) water logging: for 24 hours with distilled water immersion by the ready Dry corn stalk stalk raw material, filter residue is collected by filtration later
Spare, the filter residue is labeled as No. 1 filter residue；Soaking process requires Dry corn stalk stalk raw material described in distillation water submerged, so not only
The impurity such as dust can be removed, additionally it is possible to the Dry corn stalk stalk raw tissue softening be made to be convenient for the operation of subsequent step；
(3) dries pulverizing: No. 1 filter residue being put into thermostatic drying chamber and is dried, is crushed to 80 mesh later,
Obtain stalk powder；
(4) alkaline ethanol solution washs: taking a certain amount of stalk powder to be added in alkaline ethanol solution and slowly heats up
To 80 DEG C, 2h is reacted, filter residue is collected in filtering, is labeled as No. 2 filter residues；
(5) it washes: No. 2 filter residues being soaked in distilled water and maintain for 24 hours, to remove the wood in No. 2 filter residue tissues
Quality substance；
(6) acidic xylan enzyme hydrolysis: the substance taking-up after washing is placed in suitable container, 0.05moL/L is added
Citric acid-sodium citrate buffer solution (pH=4.8) 200mL, is put into thermostat water bath and is warming up to 50 DEG C, xylan is added later
Enzyme 0.5g, reaction time 12h obtain acidic xylan protease hydrolysate；
(7) laccase hydrolyzes: 0.2moL/L acetic acid-sodium acetate buffer solution being added in the acidic xylan protease hydrolysate
(pH=4.8) 200mL, and hydrogen peroxide 0.6mL is added, 50 DEG C are warming up to, laccase 0.1g is added later, reaction time 3h is obtained
Laccase hydrolysate；
(8) it is slow that 0.05moL/L citric acid-sodium citrate acid cellulose enzyme hydrolysis: is added in the laccase hydrolysate
Fliud flushing (pH=4.8) 200mL, is put into thermostat water bath and is warming up to 50 DEG C, and cellulase 0.05g, reaction time are added later
5h obtains acid cellulose protease hydrolysate；
(9) high speed dispersion is handled: the acid cellulose protease hydrolysate carries out centrifugal treating, collects filter residue, is labeled as No. 3
No. 3 filter residues are carried out high speed dispersion and handle 2min, obtain high speed dispersion suspension by filter residue；
(10) high-pressure homogeneous processing: the high speed dispersion suspension is put into homogenizer, handles 30s, it is equal to obtain high pressure
Matter handles suspension；
(11) decoloration washing: the high-pressure homogeneous processing suspension is filtered, and collects filter residue, is labeled as No. 4 filter residues,
The hydrogen peroxide that concentration is 0.02mol/L is added in No. 4 filter residues, reacts 30min under the conditions of 50 DEG C, washes 5 times later
The preparation of nano-cellulose fiber NFC is completed in left and right；
(12) flavor mixing is handled: being added 4g sucrose and 0.1g citric acid in the NFC, and is added appropriate pectin and carboxylic
Sodium carboxymethylcellulose pyce obtains beverage first product；
(13) it sterilizes, is cooling filling: by the way of the sterilization of low temperature bus, water bath with thermostatic control being warming up to 62 DEG C, is put into drink
Expect that first product carries out the sterilization of low temperature bus, sterilizing time 30min is cooled to room temperature filling later.
Properties of product detection:
1. sensory evaluation
According to the standards of grading of table 1, sensory evaluation is carried out to a kind of stalk functional beverage obtained in embodiment 2: outer
See limpid, the impurity such as no precipitating, suspended matter；The natural taste of beverage made of fruits or vegetables is remained, without undesirable smell, sweet mouthfeel is suitable
In, special taste；It is scored at 86.6.Use commercial product for control group, sensory evaluation is scored at 74.5.
2. nutritional ingredient is evaluated
Experimentation: according to nutritional ingredient evaluation method in embodiment 1, to a kind of stalk function of being prepared in embodiment 2
Property beverage carry out the ICP-MS measurement of the measurement of soluble sugar, microelement and heavy metal element, the analysis of amino acid content, dimension
The analysis of raw element C content.
Experimental result: know that soluble sugar is main in a kind of stalk functional beverage prepared in embodiment 2 by analysis
Fructose and sucrose two major classes, fructose content highest, maximum level is 4.8 times of sucrose maximum level, measure fructose contains
Amount is 11.87mg/mL；The content of mineral matter element K, Na, Ca, Mg, Zn, Fe and Mn element be respectively 1673,76.9., 801,
612,2.15,1.19 and 3.76mg/L, heavy metal element Cu, As, Se, Cd, Pb are not detected；16 kinds of amino are detected altogether
Acid, wherein alanine, histidine, serine, lysine, the content of threonine are more, threonine needed by human, valine,
7 kinds of methionine, phenylalanine, isoleucine, leucine, lysine amino acid contents are respectively 1.74,0.52,0.83,0.42,
0.34,0.89 and 1.12mg/100mL；Vitamin C content is relatively high, has reached 84.93 μ g/g.
Use commercially available three kinds of products for control group simultaneously, the experimental results showed that amino acid in the functional beverage of blending type
Type is less, only alanine, two class of glutamic acid, and for carbohydrate also based on sucrose, plant fermented liquid is in basic functional beverage
The type of amino acid is more, but every kind of content is relatively low.
Embodiment 3
A kind of preparation method of stalk functional beverage, comprising the following steps:
(1) Dry corn stalk stalk raw material preparation: 10g corn dry straw being cut to size 3cm or so, remove surface dust,
Impurity etc. to get arrive Dry corn stalk stalk raw material；The too long operation that will affect subsequent step of the length of stalk；
(2) water logging: for 24 hours with distilled water immersion by the ready Dry corn stalk stalk raw material, filter residue is collected by filtration later
Spare, the filter residue is labeled as No. 1 filter residue；Soaking process requires Dry corn stalk stalk raw material described in distillation water submerged, so not only
The impurity such as dust can be removed, additionally it is possible to the Dry corn stalk stalk raw tissue softening be made to be convenient for the operation of subsequent step；
(3) dries pulverizing: No. 1 filter residue being put into thermostatic drying chamber and is dried, is crushed to 80 mesh later,
Obtain stalk powder；
(4) alkaline ethanol solution washs: taking a certain amount of stalk powder to be added in alkaline ethanol solution and slowly heats up
To 80 DEG C, 2h is reacted, filter residue is collected in filtering, is labeled as No. 2 filter residues；
(5) it washes: No. 2 filter residues being soaked in distilled water and maintain for 24 hours, to remove the wood in No. 2 filter residue tissues
Quality substance；
(6) acidic xylan enzyme hydrolysis: the substance taking-up after washing is placed in suitable container, 0.05moL/L is added
Citric acid-sodium citrate buffer solution (pH=4.8) 200mL, is put into thermostat water bath and is warming up to 50 DEG C, xylan is added later
Enzyme 0.5g, reaction time 12h obtain acidic xylan protease hydrolysate；
(7) laccase hydrolyzes: 0.2moL/L acetic acid-sodium acetate buffer solution being added in the acidic xylan protease hydrolysate
(pH=4.8) 200mL, and hydrogen peroxide 0.6mL is added, 50 DEG C are warming up to, laccase 0.1g is added later, reaction time 3h is obtained
Laccase hydrolysate；
(8) it is slow that 0.05moL/L citric acid-sodium citrate acid cellulose enzyme hydrolysis: is added in the laccase hydrolysate
Fliud flushing (pH=4.8) 200mL, is put into thermostat water bath and is warming up to 50 DEG C, and cellulase 0.05g, reaction time are added later
5h obtains acid cellulose protease hydrolysate；
(9) high speed dispersion is handled: the acid cellulose protease hydrolysate carries out centrifugal treating, collects filter residue, is labeled as No. 3
No. 3 filter residues are carried out high speed dispersion and handle 2min, obtain high speed dispersion suspension by filter residue；
(10) high-pressure homogeneous processing: the high speed dispersion suspension is put into homogenizer, handles 30s, it is equal to obtain high pressure
Matter handles suspension；
(11) decoloration washing: the high-pressure homogeneous processing suspension is filtered, and collects filter residue, is labeled as No. 4 filter residues,
The hydrogen peroxide that concentration is 0.02mol/L is added in No. 4 filter residues, reacts 30min under the conditions of 50 DEG C, washes 5 times later
The preparation of nano-cellulose fiber NFC is completed in left and right；
(12) flavor mixing is handled: being added 5g sucrose and 0.2g citric acid in the NFC, and is added appropriate pectin and carboxylic
Sodium carboxymethylcellulose pyce obtains beverage first product；
(13) it sterilizes, is cooling filling: by the way of the sterilization of low temperature bus, water bath with thermostatic control being warming up to 62 DEG C, is put into drink
Expect that first product carries out the sterilization of low temperature bus, sterilizing time 30min is cooled to room temperature filling later.
Properties of product detection:
1. sensory evaluation
According to the standards of grading of table 1, sensory evaluation is carried out to a kind of stalk functional beverage obtained in embodiment 3: outer
See limpid, the impurity such as no precipitating, suspended matter；The natural taste of beverage made of fruits or vegetables is remained, without undesirable smell, sweet mouthfeel is suitable
In, special taste；It is scored at 87.0.Use commercial product for control group, sensory evaluation is scored at 74.5.
2. nutritional ingredient is evaluated
Experimentation: according to nutritional ingredient evaluation method in embodiment 1, to a kind of stalk function of being prepared in embodiment 2
Property beverage carry out the ICP-MS measurement of the measurement of soluble sugar, microelement and heavy metal element, the analysis of amino acid content, dimension
The analysis of raw element C content.
Experimental result: by the way that soluble sugar is mainly fructose and sucrose two major classes in stalk functional beverage known to analysis,
Fructose content highest, maximum level are 4.3 times of sucrose maximum level, measure fructose content be 10.87mg/mL；Mineral
The content of prime element K, Na, Ca, Mg, Zn, Fe and Mn element is respectively the and of 1654,86.7., 721,591,3.11,1.81
4.06mg/L, heavy metal element Cu, As, Se, Cd, Pb are not detected；16 kinds of amino acid are detected altogether, wherein alanine, group
Propylhomoserin, serine, lysine, the content of threonine are more, threonine needed by human, valine, methionine, phenylalanine,
7 kinds of isoleucine, leucine, lysine amino acid contents are respectively 1.74,0.56,0.41,0.17,0.23,0.64 and
1.51mg/100mL；Vitamin C content is relatively high, has reached 68.8 μ g/g.
Use commercially available three kinds of products for control group simultaneously, the experimental results showed that amino acid in the functional beverage of blending type
Type is less, only alanine, two class of glutamic acid, and for carbohydrate also based on sucrose, plant fermented liquid is in basic functional beverage
The type of amino acid is more, but every kind of content is relatively low.
It should be appreciated that described above, the specific embodiments are only for explaining the present invention, is not intended to limit the present invention.By
The obvious changes or variations that spirit of the invention is extended out are still in the protection scope of this invention.
Claims (4)
1. a kind of preparation method of stalk functional beverage, which comprises the following steps:
(1) Dry corn stalk stalk raw material preparation: corn dry straw is cut to size 3cm or so, dust, the impurity on surface etc. are removed,
Obtain Dry corn stalk stalk raw material；The too long operation that will affect subsequent step of the length of stalk；
(2) water logging: for 24 hours with distilled water immersion by the ready Dry corn stalk stalk raw material, it is spare to be collected by filtration filter residue later,
The filter residue is labeled as No. 1 filter residue；Soaking process requires Dry corn stalk stalk raw material described in distillation water submerged, not only can go
Except impurity such as dusts, additionally it is possible to soften the Dry corn stalk stalk raw tissue, convenient for the operation of subsequent step；
(3) dries pulverizing: No. 1 filter residue is put into thermostatic drying chamber and is dried, 80 mesh is crushed to later, obtained
Stalk powder；
(4) alkaline ethanol solution washs: taking a certain amount of stalk powder to be added in alkaline ethanol solution and is to slowly warm up to 80
DEG C, 2h is reacted, filter residue is collected in filtering, is labeled as No. 2 filter residues；
(5) it washes: No. 2 filter residues being soaked in distilled water and maintain for 24 hours, to remove the lignin in No. 2 filter residue tissues
Substance；
(6) acidic xylan enzyme hydrolysis: the substance taking-up after washing is placed in suitable container, according to solid-liquid ratio 1:20 ratio
0.05moL/L citric acid-sodium citrate buffer solution (pH=4.8) 200mL is added, is put into thermostat water bath and is warming up to 50~55
DEG C, zytase 0.5g is added, reaction time 12h obtains acidic xylan protease hydrolysate；
(7) laccase hydrolyzes: 0.2moL/L acetic acid-acetic acid is added according to solid-liquid ratio 1:20 in the acidic xylan protease hydrolysate
Sodium buffer (pH=4.8) 200mL, and hydrogen peroxide 0.6mL is added, 50~55 DEG C are warming up to, laccase 0.1g, reaction are added later
Time 3h obtains laccase hydrolysate；
(8) 0.05moL/L citric acid-acid cellulose enzyme hydrolysis: is added according to solid-liquid ratio 1:20 in the laccase hydrolysate
Sodium citrate buffer solution (pH=4.8) 200mL, is put into thermostat water bath and is warming up to 50~55 DEG C, cellulase is added later
0.05g, reaction time 5h obtain acid cellulose protease hydrolysate；
(9) high speed dispersion is handled: the acid cellulose protease hydrolysate carries out centrifugal treating, collects filter residue, labeled as No. 3 filters
No. 3 filter residues are carried out high speed dispersion and handle 2min, obtain high speed dispersion suspension by slag；
(10) high-pressure homogeneous processing: the high speed dispersion suspension is put into homogenizer, is handled 30s, is obtained high-pressure homogeneous place
Manage suspension；
(11) decoloration washing: the high-pressure homogeneous processing suspension is filtered, and collects filter residue, No. 4 filter residues is labeled as, in institute
The hydrogen peroxide for being added that concentration is 0.02mol/L in No. 4 filter residues is stated, reacts 30min under the conditions of 50 DEG C, is washed 5 times later, is completed
The preparation of nano-cellulose fiber NFC；
(12) flavor mixing is handled: being added a certain amount of sucrose and citric acid in the nano-cellulose fiber NFC, and is added
Appropriate pectin and sodium carboxymethylcellulose, obtain beverage first product；
(13) it sterilizes, is cooling filling: by the way of the sterilization of low temperature bus, water bath with thermostatic control being warming up to 62 DEG C, at the beginning of being put into beverage
Product carry out the sterilization of low temperature bus, and sterilizing time 30min is cooled to room temperature rear filling later.
2. a kind of preparation method of stalk functional beverage according to claim 1, which is characterized in that the dry straw of corn
The mass ratio of stalk, sucrose and citric acid is 50:20:1.
3. a kind of preparation method of stalk functional beverage according to claim 1 or claim 2, which is characterized in that the acid wood
Glycanase hydrolysis, laccase hydrolyze, the temperature of acid cellulose enzyme hydrolysis is all 50 DEG C.
4. a kind of stalk functional beverage, which is characterized in that a kind of stalk functional beverage according to claim 1 to 3
Preparation method is prepared.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811472788.0A CN109380634A (en) | 2018-12-04 | 2018-12-04 | A kind of stalk functional beverage and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811472788.0A CN109380634A (en) | 2018-12-04 | 2018-12-04 | A kind of stalk functional beverage and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109380634A true CN109380634A (en) | 2019-02-26 |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103741532A (en) * | 2013-12-23 | 2014-04-23 | 江南大学 | Preparation method for nano cellulosic fibers strong in grease and cholate adsorption |
| CN103981237A (en) * | 2014-05-29 | 2014-08-13 | 江南大学 | Method for preparing xylooligosaccharide, lignin and microcrystalline cellulose by completely utilizing straws |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103741532A (en) * | 2013-12-23 | 2014-04-23 | 江南大学 | Preparation method for nano cellulosic fibers strong in grease and cholate adsorption |
| CN103981237A (en) * | 2014-05-29 | 2014-08-13 | 江南大学 | Method for preparing xylooligosaccharide, lignin and microcrystalline cellulose by completely utilizing straws |
Non-Patent Citations (1)
| Title |
|---|
| 范娟等: "玉米秸秆饮料营养成分与生产工艺流程研究", 《江苏农业科学》 * |
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Application publication date: 20190226 |