CN109369913A - PH-responsive polyaspartic acid grafted with hydrophobic amino acid and preparation method thereof - Google Patents
PH-responsive polyaspartic acid grafted with hydrophobic amino acid and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a pH responsive polyaspartic acid grafted with hydrophobic amino acid and a preparation method thereof, wherein polyaspartic acid-grafted-phenylalanine is obtained by taking polyaspartic acid and L-phenylalanine methyl ester hydrochloride as raw materials through 1- (3-dimethylaminopropyl) -3-ethylcarbodiimide coupling reaction and alkali washing; the method comprises the following steps of taking polyaspartic acid-graft-phenylalanine and hydrophobic amino acid hydrochloride as raw materials, and carrying out coupling reaction and alkali washing to obtain a product. The hydrochloride salt of a hydrophobic amino acid comprises Nε-benzyloxycarbonyl lysine benzyl ester hydrochloride, tryptophan methyl ester hydrochloride, valine methyl ester hydrochloride and isoleucine methyl ester hydrochloride; two amino acids are continuously grafted on the side group, the polymer contains anionic carboxyl with pH responsiveness, the biocompatibility is good, and the survival rate of smooth muscle cells is over 80% when the concentration of the polymer is less than 0.1 mg/mL. Simple operation and low cost. Is applicable to the field of biomedical drug carrier materials.
Description
Technical field
The present invention relates to a kind of pH responsiveness poly-aspartate of grafted hydrophobic acidic amino acid and preparation methods, and in particular to
The preparation and its application of the pH responsiveness polyaminoacid of poly-aspartate grafted hydrophobic acidic amino acid belong to bio-medical drug load
Body Material Field.
Background technique
Change with solution ph, the conformation of pH responsive polymer can occur to change accordingly.Currently, poly- to pH responsiveness
Close research existing a large amount of report (Kanamala M, Wilson WR, Yang M, the Palmer BD, Wu of object
Z.Mechanisms and biomaterials in pH-responsive tumor targeted drug delivery:A
review.Biomaterials,2016,85,152-167).PH responsive polymer can be divided into according to its structure intermediate ion attribute
Anionic polymer and cationic polymer.Wherein, pH responsiveness anionic polymer has poly-aspartate, polyacrylic acid, gathers
Ethylacrylic acid and polymethylacrylic acid, polysulfonamide etc.；And pH responsiveness cationic polymer has polylysine, polyhistidyl
Deng.In addition, also thering is the block copolymer of pH responsiveness to report, such as polyethylene glycol-b- polyglutamic acid-b- poly sarcosine, such pH
Responsive polymer has important application value in bio-medical field, such as the delivering of tumor-targeting drug.
In recent years, it the preparation of amphipathic amino acid grafted hydrophobic compound and its nanoparticle and is answered as pharmaceutical carrier
Research report is more and more.Chinese patent (105492494 B of CN) discloses polyaminoacid and hydrophobicity primary amino-compound
Graft copolymer, and the graft product nanoparticle of obtained poly- (gamma-glutamic acid) and phenylalanine ethyl ester is used as vaccine
Adjuvant.Chinese patent (102875818 B of CN) discloses the graft copolymer of polyaminoacid Yu hydrophily and hydrophobic compound,
Such as polyglutamic acid, the fatty alcohol of Polysucciuimide grafting C8~C30, which can wrap up in an aqueous medium
Drug, the IV delivery suitable for drug.In addition, Chinese patent (102369242 B of CN) discloses one kind containing hydrophobization acid
Property polyaminoacid and basic polypeptide poly ion complexes, which can be used as immune system with antigen binding at nanoparticle
Agent.
Therefore, as the pH responsive polymer of drug delivery, still there is life after poly-aspartate grafted hydrophobic amino acid
The pH responsiveness that the amphipathic and anionic carboxyl that object degradability and biocompatibility and hydrophobe group provide provides, phase
For cation amino polymer, the cytotoxicity of such anionic carboxylic polymers is relatively small.That reports at present is this kind of
Anionic polypeptide is mostly the single hydrophobic amino acid esters of side chain graft, has no the report of continuously two kinds of hydrophobic amino acids of grafting
Road.
Summary of the invention
The purpose of the present invention is intended to provide pH responsiveness poly-aspartate and the preparation side of a kind of grafted hydrophobic acidic amino acid
Method, after this method is using degradable poly-aspartate grafting phenylalanine, continuous grafted hydrophobic amino acid prepares pH responsiveness
Amphipathic amino acid bio material, enrich Polysucciuimide grafted hydrophobic amino acid structure type, and easy to operate, reaction
Mild condition, experimental raw are easy to get.The poly-aspartate of the grafted hydrophobic amino acid has good biocompatibility and pH
Responsiveness is suitable for bio-medical drug carrier material field.
Technical scheme is as follows:
A kind of pH responsiveness poly-aspartate of grafted hydrophobic acidic amino acid, structural formula are as follows:
In formula (I), R NεBenzyloxycarbonyl group lysine or tryptophan or valine or isoleucine；N=39~800；
X >=0, y >=0, z >=0 and x, y and z cannot be 0 simultaneously；n>x+y>0,n>x+y+z>0.
The preparation method of the poly-aspartate of grafted hydrophobic amino acid of the invention passes through using poly-aspartate as raw material
1- (3- dimethylaminopropyl) -3- ethyl carbodiimide coupling reaction, after being grafted L-phenylalanine methyl ester hydrochloride, alkali cleaning is obtained
To poly-aspartate-grafting-phenylalanine；
Further using poly-aspartate-grafting-phenylalanine as raw material, pass through 1- (3- dimethylaminopropyl) -3- second
Base carbodiimide coupling reaction, grafted hydrophobic amino acid methyl ester hydrochloride (NεBenzyloxycarbonyl group lysine methyl ester hydrochloride, color ammonia
Acid methyl ester hydrochloride salt, valine methyl ester hydrochloride or Isoleucine methyl ester hydrochloride), alkali cleaning obtains the poly- of grafted hydrophobic amino acid
Aspartic acid product.
Wherein, the molar ratio that the carboxyl of poly-aspartate and L-phenylalanine methyl esters feed intake is 1:0.6~1.2；Poly- asparagus fern
The molar ratio that propylhomoserin-grafting-phenylalanine carboxyl and hydrophobic amino acid methyl esters feed intake is 1:0.5~1.2；Poly-aspartate carboxylic
Base and 1- (3- dimethylaminopropyl) -3- ethyl carbodiimide molar ratio are 1:0.5~1.0.
Wherein, several hydrophobic amino acid methyl ester hydrochloride (NεBenzyloxycarbonyl group lysine methyl ester hydrochloride, tryptophan methyl ester
Hydrochloride, valine methyl ester hydrochloride or Isoleucine methyl ester hydrochloride) respectively with poly-aspartate-grafting-phenylalanine into
Row reaction, respectively obtains Polysucciuimide-grafting-phenylalanine-grafting-benzyloxycarbonyl group lysine, Polysucciuimide-grafting-
Phenylalanine-grafting-tryptophan, Polysucciuimide-grafting-phenylalanine-grafting-valine and Polysucciuimide-grafting-benzene
Alanine-grafting-isoleucine；Wherein, the amino of above-mentioned four kinds of hydrophobic amino acids respectively with Polysucciuimide-grafting-phenylpropyl alcohol
The carboxyl of propylhomoserin strand side group carries out random coupling reaction, and after the reaction was completed, hydrophobic amino acid is in polymer side base location
Random, concrete structure formula is such as shown in (I).
The preparation method of the pH responsiveness poly-aspartate of grafted hydrophobic acidic amino acid of the invention, steps are as follows:
1) it takes water as a solvent, configuration quality concentration is the sodium bicarbonate aqueous solution of 0.21~0.63wt%, then by poly- day
Carboxyl-content and 1- (3- dimethylaminopropyl) -3- ethyl carbodiimide of aspartic acid is dissolved according to molar ratio 1:0.5~1.0
In sodium bicarbonate aqueous solution, the solution that poly-aspartate concentration is 1~2wt% is obtained, 30~60min is stirred；
2) poly-aspartate carboxyl and L-phenylalanine methyl esters molar ratio 1:0.6~1.2 are pressed, L-phenylalanine methyl esters is added
Hydrochloric acid saline solution is reacting at room temperature 48~72h；
3) concentration is added is the ethanol solution alkali cleaning of 2.0~5.0wt% sodium hydroxide, is stirred overnight at room temperature, wherein hydrogen-oxygen
The volume fraction for changing sodium ethoxide solution relative response system is 50~80%；
4) solution is fitted into the bag filter of 0.5~1kDa molecular cut off, with deionized water dialysis 3~5 days, after freeze-drying
Poly-aspartate-grafting-phenylalanine is obtained, such as formula (II).
T is poly-aspartate-grafting-phenylalanine number of repeat unit, x+y=t in formula (II)；n>t>0；N=39~
800。
5) it regard the poly-aspartate of step 4)-grafting-phenylalanine (II) as raw material, the carboxyl of poly-aspartate is contained
Amount and 1- (3- dimethylaminopropyl) -3- ethyl carbodiimide 1:0.5~1.0 in molar ratio；It is dense to be dissolved in sodium bicarbonate quality
Degree be 0.21~0.63wt% sodium bicarbonate aqueous solution in, obtain poly-aspartate-grafting-concentration of phenylalanine be 0.5~
The solution of 1.0wt%；30~60min is activated at room temperature；
6) by poly-aspartate-grafting-phenylalanine carboxyl and hydrophobic amino acid molar ratio 1:0.1~1.2, add hydrophobic
Amino acid methyl ester hydrochloride (NεBenzyloxycarbonyl group lysine methyl ester hydrochloride, tryptophan methyl ester hydrochloride, valine methyl ester hydrochloric acid
Salt or Isoleucine methyl ester hydrochloride) aqueous solution, in room temperature the reaction was continued 48-72h；
7) concentration is added is the ethanol solution alkali cleaning of 2.0~5.0wt% sodium hydroxide, is stirred overnight at room temperature, wherein hydrogen-oxygen
The volume fraction for changing sodium ethoxide solution relative response system is 50~80%；
8) it uses deionized water dialysis solution 3~5 days, obtains poly-aspartate-grafting-hydrophobic amino after 24~48h is lyophilized
Acid, such as formula (I).
Poly-aspartate-grafting-the hydrophobic amino acid is suitable for bio-medical drug carrier material field.
The present invention passes through 1- (3- dimethylamino third using poly-aspartate and L-phenylalanine methyl ester hydrochloride as raw material
Base) -3- ethyl carbodiimide coupling reaction and alkali cleaning, obtain poly-aspartate-grafting-phenylalanine；Again with poly-aspartate-
Grafting-phenylalanine and hydrophobic amino acid are raw material, by coupling reaction simultaneously alkali cleaning, obtain product.The advantages of this method is
Easy to operate, at low cost, reaction condition is mild, and experimental raw is easy to get.Poly-aspartate-grafting-hydrophobic amino of the invention
The hydrophilic and hydrophobic of acid is adjustable, has pH responsiveness and good cell compatibility.This pH responsiveness poly-aspartate-grafting-
Hydrophobic amino acid is suitable for bio-medical drug carrier material field.
Specific embodiment
Below by case study on implementation to technical solution of the present invention further description, following case study on implementation is to the present invention
Further explanation, be not intended to limit the scope of application of the invention.
Embodiment 1:
(1) poly-aspartate-grafting-phenylalanine-grafting-benzyloxycarbonyl group lysine preparation
1) by poly-aspartate (molecular weight Mw=4500) (3.00g, the amount of carboxyl substance are 0.026mol) and 1- (3- bis-
Dimethylaminopropyl) -3- ethyl carbodiimide 4.98g, 0.026mol) it is dissolved in the carbonic acid that 150mL mass concentration is 0.63wt%
In hydrogen sodium water solution, the solution that poly-aspartate concentration is 2.0wt% is obtained, 30min is stirred；
2) poly-aspartate carboxyl and L-phenylalanine methyl ester hydrochloride molar ratio 1:1.2 are pressed, L-phenylalanine first is added
Ester hydrochloride (6.69g, 0.031mol) aqueous solution is in room temperature reaction 72h；
3) 75mL concentration is added is the ethanol solution alkali cleaning of 5.0wt% sodium hydroxide, is stirred overnight at room temperature；
4) solution is fitted into the bag filter of molecular cut off 0.5kDa, with deionized water dialysis 3 days, is purified, frozen
Dry to obtain poly-aspartate-grafting-phenylalanine afterwards for 24 hours, phenylalanine grafting rate is 71.8%, as structural formula is as follows:
5) using poly-aspartate-grafting-phenylalanine of step 4) as raw material, in the 250mL that magnetic stir bar is housed
In single-necked flask, it is added poly-aspartate-grafting-phenylalanine (0.5g, the amount 0.0023mol of carboxyl substance), 1- (3- diformazan
Base aminopropyl) -3- ethyl carbodiimide (0.44g, 0.0023mol), 100mL sodium bicarbonate aqueous solution (0.42wt%) obtains
The solution for being 0.5wt% to poly-aspartate-grafting-concentration of phenylalanine；60min is activated at room temperature；
6) by poly-aspartate-grafting-phenylalanine carboxyl and hydrophobic amino acid molar ratio 1:1.2, add benzyloxycarbonyl group
Lysine methyl ester hydrochloride (1.14g, 0.0028mol) aqueous solution, in room temperature the reaction was continued 56h；
7) 50mL concentration is added is the ethanol solution alkali cleaning of 3.0wt% sodium hydroxide, is stirred overnight at room temperature；
8) solution is fitted into the bag filter of molecular cut off 1kDa, with deionized water dialysis solution 4 days, after 48h is lyophilized
Poly-aspartate-grafting-phenylalanine-grafting-benzyloxycarbonyl group lysine is obtained, yield is about 79%, benzyloxycarbonyl group lysine
Grafting rate is 41.1%, and structural formula is as follows:
Wherein, x+y=28；X+z=16.
(2) poly-aspartate-grafting-phenylalanine-grafting-tryptophan preparation
1) it regard step 4) product poly-aspartate-grafting-phenylalanine (II) in (1) as raw material, is stirred equipped with magnetic force
In the 250mL single-necked flask for mixing son, poly-aspartate-grafting-phenylalanine (1.0g, the amount of carboxyl substance is added
0.0046mol), 1- (3- dimethylaminopropyl) -3- ethyl carbodiimide (0.44g, 0.0023mol), 100mL sodium bicarbonate
Aqueous solution (0.21wt%) obtains the solution that poly-aspartate-grafting-concentration of phenylalanine is 1wt%；It activates at room temperature
30min；
2) by poly-aspartate-grafting-phenylalanine carboxyl and hydrophobic amino acid molar ratio 1:0.5, additive color propylhomoserin first
Ester hydrochloride (0.59g, 0.0023mol) aqueous solution, in room temperature the reaction was continued 48h；
3) 80mL concentration is added is the ethanol solution alkali cleaning of 2.0wt% sodium hydroxide, is stirred overnight at room temperature；
4) solution is fitted into the bag filter of molecular cut off 0.8kDa, freeze-drying obtains poly-aspartate-grafting-afterwards for 24 hours
Phenylalanine-grafting-tryptophan, yield are about 75%, and tryptophan grafting rate is 28.2%, and structural formula is as follows:
Wherein, x+y=28；X+z=11.
(3) poly-aspartate-grafting-phenylalanine-grafting-valine preparation
1) it regard step 4) product poly-aspartate-grafting-phenylalanine (II) in (1) as raw material, is stirred equipped with magnetic force
In the 250mL single-necked flask for mixing son, poly-aspartate-grafting-phenylalanine (0.75g, the amount of carboxyl substance is added
0.0031mol), 1- (3- dimethylaminopropyl) -3- ethyl carbodiimide (0.45g, 0.0024mol), 100mL sodium bicarbonate
Aqueous solution (0.45wt%) obtains the solution that poly-aspartate-grafting-concentration of phenylalanine is 0.75wt%；It activates at room temperature
45min；
2) by poly-aspartate-grafting-phenylalanine carboxyl and hydrophobic amino acid molar ratio 1:0.6, add valine first
Ester hydrochloride (0.31g, 0.0019mol) aqueous solution, in room temperature the reaction was continued 56h；
3) 80mL concentration is added is the ethanol solution alkali cleaning of 4.0wt% sodium hydroxide, is stirred overnight at room temperature；
4) solution is fitted into the bag filter of molecular cut off 1kDa, obtains poly-aspartate-grafting-benzene after 48h is lyophilized
Alanine-grafting-valine, yield are about 78%, and valine grafting rate is 35.9%, and structural formula is as follows:
X+y=28；X+z=14
(4) poly-aspartate-grafting-phenylalanine-grafting-isoleucine preparation
1) it regard step 4) product poly-aspartate-grafting-phenylalanine (II) in (1) as raw material, is stirred equipped with magnetic force
In the 250mL single-necked flask for mixing son, poly-aspartate-grafting-phenylalanine (0.80g, the amount of carboxyl substance is added
0.0036mol), 1- (3- dimethylaminopropyl) -3- ethyl carbodiimide (0.70g, 0.0036mol), 100mL sodium bicarbonate
Aqueous solution (0.50wt%) obtains the solution that poly-aspartate-grafting-concentration of phenylalanine is 0.8wt%；It activates at room temperature
50min；
2) by poly-aspartate-grafting-phenylalanine carboxyl and hydrophobic amino acid molar ratio 1:0.8, add isoleucine
Methyl ester hydrochloride (0.53g, 0.0029mol) aqueous solution, in room temperature the reaction was continued 54h；
3) 60mL concentration is added is the ethanol solution alkali cleaning of 4.5wt% sodium hydroxide, is stirred overnight at room temperature；
4) solution is fitted into the bag filter of molecular cut off 0.8kDa, obtains poly-aspartate-grafting-after 36h is lyophilized
Phenylalanine-grafting-isoleucine, yield are about 79%, and isoleucine grafting rate is 30.8%, and structural formula is as follows:
X+y=28；X+z=12
(5) performance test
Poly-aspartate-grafting-phenylalanine-grafting-benzyloxycarbonyl group lysine of preparation has pH responsiveness, is in pH
Clear solution when 7.4, when pH is less than 5.8, solution becomes emulsion, and polymer aggregational forms particle；Using Alamar Blue
Kit carries out cytotoxicity experiment, when polymer concentration is less than 0.1mg/mL, to the survival rate of smooth muscle cell all 80%
More than, show that poly-aspartate-grafting-phenylalanine-grafting-benzyloxycarbonyl group lysine has good cell compatibility.
Poly-aspartate-grafting-phenylalanine-grafting-tryptophan of preparation is 1.0mg/mL with concentration, is 4.8- in pH
Between 7.4 when self assembly, when pH value is greater than 5.5, solution becomes clear solution from emulsion.The poly-aspartate-of preparation connects
Branch-phenylalanine-grafting-tryptophan carries out cytotoxicity using Alamar Blue cell Proliferation/citotoxicity detection kit
Test experience, when concentration is less than 0.2mg/mL, smooth muscle cell survival rate is 80% or more.
Poly-aspartate-grafting-phenylalanine-grafting-valine of preparation is 1.0mg/mL with concentration, is 4.4- in pH
Between 7.4 when self assembly, when pH is greater than 4.4, solution becomes clear solution from emulsion.Poly-aspartate-grafting-of preparation
Phenylalanine-grafting-valine carries out cytotoxicity inspection using Alamar Blue cell Proliferation/citotoxicity detection kit
Experiment is surveyed, when concentration is less than 1.2mg/mL, smooth muscle cell survival rate is 80% or more.
Poly-aspartate-grafting-phenylalanine-grafting-isoleucine of preparation is 1.0mg/mL with concentration, is in pH
Between 4.6-7.4 when self assembly, when pH is greater than 4.6, solution becomes clear solution from emulsion.The poly-aspartate-of preparation
Grafting-phenylalanine-grafting-isoleucine using AB reagent carry out cytotoxicity test experience, when concentration be less than 1.5mg/mL,
Smooth muscle cell survival rate is 80% or more.
Embodiment 2:
(1) poly-aspartate-grafting-phenylalanine-grafting-benzyloxycarbonyl group lysine preparation
1) by poly-aspartate (molecular weight Mw=46100) (1.50g, the amount of carboxyl substance are 0.013mol) and 1- (3-
Dimethylaminopropyl) -3- ethyl carbodiimide 4.98g, 0.026mol) it is dissolved in the carbon that 150mL mass concentration is 0.21wt%
Sour hydrogen sodium water solution obtains the solution that poly-aspartate concentration is 1.0wt%, stirs 60min；
2) poly-aspartate carboxyl and L-phenylalanine methyl ester hydrochloride molar ratio 1:0.6 are pressed, L-phenylalanine first is added
Ester hydrochloride (1.68g, 0.0078mol) aqueous solution is in room temperature reaction 72h；
3) 80mL concentration is added is the ethanol solution alkali cleaning of 2.0wt% sodium hydroxide, is stirred overnight at room temperature；
4) solution is fitted into the bag filter of molecular cut off 1kDa, with deionized water dialysis 3 days, is purified, is lyophilized
Obtain poly-aspartate-grafting-phenylalanine afterwards for 24 hours, phenylalanine grafting rate is 31.5%, as structural formula is as follows:
5) using poly-aspartate-grafting-phenylalanine of step 4) as raw material, in the 250mL that magnetic stir bar is housed
In single-necked flask, it is added poly-aspartate-grafting-phenylalanine (1.0g, the amount 0.0062mol of carboxyl substance), 1- (3- diformazan
Base aminopropyl) -3- ethyl carbodiimide (1.19g, 0.0062mol), 100mL sodium bicarbonate aqueous solution (0.42wt%) obtains
The solution for being 1.0wt% to poly-aspartate-grafting-concentration of phenylalanine；45min is activated at room temperature；
6) by poly-aspartate-grafting-phenylalanine carboxyl and hydrophobic amino acid molar ratio 1:1.2, benzyloxycarbonyl group is added to rely
Propylhomoserin methyl ester hydrochloride (3.01g, 0.0074mol) aqueous solution, in room temperature the reaction was continued 60h；
7) 50mL concentration is added is the ethanol solution alkali cleaning of 5.0wt% sodium hydroxide, is stirred overnight at room temperature；
8) solution is fitted into the bag filter of molecular cut off 0.5kDa, is dialysed 5 days, obtain poly- asparagus fern ammonia after 48h is lyophilized
Acid-grafting-phenylalanine-grafting-benzyloxycarbonyl group lysine, yield is about 78%, and benzyloxycarbonyl group lysine grafting rate is
27.5%, structural formula is as follows:
Wherein, x+y=126；X+z=110.
(2) poly-aspartate-grafting-phenylalanine-grafting-tryptophan preparation
1) using poly-aspartate-grafting-phenylalanine of step 4) as raw material, in the 250mL that magnetic stir bar is housed
In single-necked flask, it is added poly-aspartate-grafting-phenylalanine (1.0g, the amount 0.0062mol of carboxyl substance), 1- (3- diformazan
Base aminopropyl) -3- ethyl carbodiimide (1.19g, 0.0062mol), 100mL sodium bicarbonate aqueous solution (0.42wt%) obtains
The solution for being 1.0wt% to poly-aspartate-grafting-concentration of phenylalanine；45min is activated at room temperature；
2) by poly-aspartate-grafting-phenylalanine carboxyl and hydrophobic amino acid molar ratio 1:1.2, add tryptophan methyl ester
Hydrochloride (1.89g, 0.0074mol) aqueous solution, in room temperature the reaction was continued 60h；
3) 50mL concentration is added is the ethanol solution alkali cleaning of 5.0wt% sodium hydroxide, is stirred overnight at room temperature；
4) solution is fitted into the bag filter of molecular cut off 0.5kDa, is dialysed 3 days, freeze-drying obtains poly- asparagus fern ammonia afterwards for 24 hours
Acid-grafting-phenylalanine-grafting-benzyloxycarbonyl group lysine, yield is about 73%, and benzyloxycarbonyl group lysine grafting rate is
31.0%, structural formula is as follows:
Wherein, x+y=126；X+z=124.
(3) poly-aspartate-grafting-phenylalanine-grafting-valine preparation
1) it regard step 4) product poly-aspartate-grafting-phenylalanine (II) in (1) as raw material, is stirred equipped with magnetic force
In the 250mL single-necked flask for mixing son, poly-aspartate-grafting-phenylalanine (0.60g, the amount of carboxyl substance is added
0.0037mol), 1- (3- dimethylaminopropyl) -3- ethyl carbodiimide (0.36g, 0.0019mol), 100mL sodium bicarbonate
Aqueous solution (0.32wt%) obtains the solution that poly-aspartate-grafting-concentration of phenylalanine is 0.6wt%；It activates at room temperature
30min；
2) by poly-aspartate-grafting-phenylalanine carboxyl and hydrophobic amino acid molar ratio 1:0.4, add valine first
Ester hydrochloride (0.31g, 0.0015mol) aqueous solution, in room temperature the reaction was continued 48h；
3) 60mL concentration is added is the ethanol solution alkali cleaning of 3.0wt% sodium hydroxide, is stirred overnight at room temperature；
4) solution is fitted into the bag filter of molecular cut off 0.5kDa, dialyses 4 days, poly-aspartate-is obtained after freeze-drying
Grafting-phenylalanine-grafting-valine, yield is about 65%, and valine grafting rate is 37.1%, and structural formula is as follows:
X+y=126；X+z=148
(4) poly-aspartate-grafting-phenylalanine-grafting-isoleucine preparation
1) it regard step 4) product poly-aspartate-grafting-phenylalanine (II) in (1) as raw material, is stirred equipped with magnetic force
In the 250mL single-necked flask for mixing son, poly-aspartate-grafting-phenylalanine (0.90g, the amount of carboxyl substance is added
0.0056mol), 1- (3- dimethylaminopropyl) -3- ethyl carbodiimide (0.96g, 0.0050mol), 100mL sodium bicarbonate
Aqueous solution (0.40wt%) obtains the solution that poly-aspartate-grafting-concentration of phenylalanine is 0.9wt%；It activates at room temperature
50min；
2) by poly-aspartate-grafting-phenylalanine carboxyl and hydrophobic amino acid molar ratio 1:0.7, add isoleucine first
Ester hydrochloride (0.71g, 0.0039mol) aqueous solution, in room temperature the reaction was continued 48h；
3) 50mL concentration is added is the ethanol solution alkali cleaning of 4.8wt% sodium hydroxide, is stirred overnight at room temperature；
4) solution is fitted into the bag filter of molecular cut off 0.5kDa, is dialysed 5 days, freeze-drying obtains poly- asparagus fern ammonia afterwards for 24 hours
Acid-grafting-phenylalanine-grafting-isoleucine, yield is about 77%, and isoleucine grafting rate is 30.8%, and structural formula is such as
Under:
X+y=126；X+z=123
(5) performance test
Poly-aspartate-grafting-phenylalanine-grafting-benzyloxycarbonyl group lysine of preparation has pH responsiveness, in pH value
Clear solution when 7.4, polymer molecular chain are extended position, and when pH value is less than 5.9, solution becomes emulsion；Using Alamar
Blue kit carries out cytotoxicity experiment and all exists when polymer concentration is less than 0.1mg/mL to the survival rate of smooth muscle cell
80% or more, show that poly-aspartate-grafting-phenylalanine-grafting-benzyloxycarbonyl group lysine has good cytocompatibility
Property.
Poly-aspartate-grafting-phenylalanine-grafting-tryptophan of preparation is 1.0mg/mL with concentration, is 4.9- in pH
Between 7.4 when self assembly, when pH is greater than 5.4, solution becomes clear solution from emulsion.Poly-aspartate-grafting-of preparation
Phenylalanine-grafting-tryptophan carries out cytotoxicity inspection using Alamar Blue cell Proliferation/citotoxicity detection kit
Experiment is surveyed, when concentration is less than 0.3mg/mL, smooth muscle cell survival rate is 80% or more.
Poly-aspartate-grafting-phenylalanine-grafting-valine of preparation is 1.0mg/mL with concentration, is 4.8- in pH
Between 7.4 when self assembly, when pH is greater than 5.0, solution becomes clear solution from emulsion.Poly-aspartate-grafting-of preparation
Phenylalanine-grafting-valine carries out cytotoxicity inspection using Alamar Blue cell Proliferation/citotoxicity detection kit
Experiment is surveyed, when concentration is less than 1.3mg/mL, smooth muscle cell survival rate is 80% or more.
Poly-aspartate-grafting-phenylalanine-grafting-isoleucine of preparation is 1.0mg/mL with concentration, is in pH
Between 4.6~7.4 when self assembly, when pH is greater than 5.2, solution becomes clear solution from emulsion.The poly-aspartate-of preparation
Grafting-phenylalanine-grafting-isoleucine using AB reagent carry out cytotoxicity test experience, when concentration be less than 1.7mg/mL,
Smooth muscle cell survival rate is 80% or more.
Embodiment 3:
(1) poly-aspartate-grafting-phenylalanine-grafting-benzyloxycarbonyl group lysine preparation
1) by poly-aspartate (molecular weight Mw=16800) (2.0g, the amount of carboxyl substance are 0.017mol) and 1- (3- bis-
Dimethylaminopropyl) -3- ethyl carbodiimide 2.3g, 0.012mol) it is dissolved in the bicarbonate that 100mL mass concentration is 0.54wt%
Sodium water solution obtains the solution that poly-aspartate concentration is 2.0wt%, stirs 50min；
2) poly-aspartate carboxyl and L-phenylalanine methyl ester hydrochloride molar ratio 1:0.9 are pressed, L-phenylalanine first is added
Ester hydrochloride (3.33g, 0.015mol) aqueous solution is in room temperature reaction 72h；
3) 50mL concentration is added is the ethanol solution alkali cleaning of 4.0wt% sodium hydroxide, is stirred overnight at room temperature；
4) solution is fitted into the bag filter of molecular cut off 1kDa, dialyses 3 days, is purified, freeze-drying is gathered afterwards for 24 hours
Aspartic acid-grafting-phenylalanine, phenylalanine grafting rate is 43.1%, as structural formula is as follows:
5) using poly-aspartate-grafting-phenylalanine of step 4) as raw material, in the 250mL that magnetic stir bar is housed
In single-necked flask, it is added poly-aspartate-grafting-phenylalanine (1.0g, the amount 0.0056mol of carboxyl substance), 1- (3- diformazan
Base aminopropyl) -3- ethyl carbodiimide (0.86g, 0.0045mol), 100mL sodium bicarbonate aqueous solution (0.63wt%) obtains
The solution for being 1.0wt% to poly-aspartate-grafting-concentration of phenylalanine；54min is activated at room temperature；
6) by poly-aspartate-grafting-phenylalanine carboxyl and hydrophobic amino acid molar ratio 1:1, add benzyloxycarbonyl group propylhomoserin
Methyl ester hydrochloride (2.28g, 0.0056mol) aqueous solution, in room temperature the reaction was continued 72h；
7) 50mL concentration is added is the ethanol solution alkali cleaning of 4.2wt% sodium hydroxide, is stirred overnight at room temperature；
8) it uses deionized water dialysis solution three days, poly-aspartate-grafting-phenylalanine-grafting-benzyloxy is obtained after freeze-drying
Carbonyl lysine, yield are about 69%, and benzyloxycarbonyl group lysine grafting rate is 23.6%, and structural formula is as follows:
Wherein, x+y=62；X+z=34.
(2) poly-aspartate-grafting-phenylalanine-grafting-tryptophan preparation
1) using poly-aspartate-grafting-phenylalanine of step 4) as raw material, in the 250mL that magnetic stir bar is housed
In single-necked flask, it is added poly-aspartate-grafting-phenylalanine (0.8g, the amount 0.0045mol of carboxyl substance), 1- (3- diformazan
Base aminopropyl) -3- ethyl carbodiimide (0.52g, 0.0027mol), 100mL sodium bicarbonate aqueous solution (0.36wt%) obtains
The solution for being 0.8wt% to poly-aspartate-grafting-concentration of phenylalanine；30min is activated at room temperature；
2) by poly-aspartate-grafting-phenylalanine carboxyl and hydrophobic amino acid molar ratio 1:0.7, additive color propylhomoserin first
Ester hydrochloride (0.80g, 0.0032mol) aqueous solution, in room temperature the reaction was continued 48h；
3) 50mL concentration is added is the ethanol solution alkali cleaning of 4.0wt% sodium hydroxide, is stirred overnight at room temperature；
4) solution is fitted into the bag filter of molecular cut off 1kDa, is dialysed 3 days, obtain poly-aspartate-after 48h is lyophilized
Grafting-phenylalanine-benzyloxycarbonyl group lysine, yield are about 71%, and benzyloxycarbonyl group lysine grafting rate is 37.5%, structural formula
It is as follows:
Wherein, x+y=62；X+z=54.
(3) poly-aspartate-grafting-phenylalanine-grafting-valine preparation
1) it regard step 4) product poly-aspartate-grafting-phenylalanine (II) in (1) as raw material, is stirred equipped with magnetic force
In the 250mL single-necked flask for mixing son, poly-aspartate-grafting-phenylalanine (0.60g, the amount of carboxyl substance is added
0.0037mol), 1- (3- dimethylaminopropyl) -3- ethyl carbodiimide (0.71g, 0.0037mol), 100mL sodium bicarbonate
Aqueous solution (0.41wt%) obtains the solution that poly-aspartate-grafting-concentration of phenylalanine is 0.6wt%；It activates at room temperature
45min；
2) by poly-aspartate-grafting-phenylalanine carboxyl and hydrophobic amino acid molar ratio 1:0.3, add valine first
Ester hydrochloride (0.19g, 0.0011mol) aqueous solution, in room temperature the reaction was continued 54h；
3) 60mL concentration is added is the ethanol solution alkali cleaning of 2.0wt% sodium hydroxide, is stirred overnight at room temperature；
4) solution is fitted into the bag filter of molecular cut off 1kDa, is dialysed 3 days, freeze-drying obtains poly- grafting-benzene afterwards for 24 hours
Alanine-grafting-valine, yield are about 68%, and valine grafting rate is 25.0%, and structural formula is as follows:
X+y=62；X+z=36
(4) poly-aspartate-grafting-phenylalanine-grafting-isoleucine preparation
1) it regard step 4) product poly-aspartate-grafting-phenylalanine (II) in (1) as raw material, is stirred equipped with magnetic force
In the 250mL single-necked flask for mixing son, poly-aspartate-grafting-phenylalanine (0.90g, the amount of carboxyl substance is added
0.0056mol), 1- (3- dimethylaminopropyl) -3- ethyl carbodiimide (0.75g, 0.0039mol), 100mL sodium bicarbonate
Aqueous solution (0.35wt%) obtains the solution that poly-aspartate-grafting-concentration of phenylalanine is 0.9wt%；It activates at room temperature
60min；
2) by carboxyl and hydrophobic amino acid molar ratio 1:0.8, add Isoleucine methyl ester hydrochloride (0.81g, 0.0045mol)
Aqueous solution, in room temperature the reaction was continued 56h；
3) 50mL concentration is added is the ethanol solution alkali cleaning of 5.0wt% sodium hydroxide, is stirred overnight at room temperature；
4) solution is fitted into the bag filter of molecular cut off 1kDa, is dialysed 4 days, freeze-drying obtains poly-aspartate-afterwards for 24 hours
Grafting-phenylalanine-grafting-isoleucine, yield is about 74%, and isoleucine grafting rate is 28.5%, and structural formula is as follows:
X+y=62；X+z=41
(5) performance test
Poly-aspartate-grafting-phenylalanine-grafting-benzyloxycarbonyl group lysine of preparation has pH responsiveness, is in pH
Clear solution when 7.4, when pH is less than 5.8, solution becomes emulsion；It is real that cytotoxicity is carried out using Alamar Blue kit
It tests, when polymer concentration is less than 0.1mg/mL, to the survival rate of smooth muscle cell all 80% or more, shows poly-aspartate-
Grafting-phenylalanine-grafting-benzyloxycarbonyl group lysine has good cell compatibility.
Poly-aspartate-grafting-phenylalanine-grafting-tryptophan of preparation is 1.0mg/mL with concentration, is 4.8- in pH
Between 7.4 when self assembly, when pH is greater than 5.1, solution becomes clear solution from emulsion.Poly-aspartate-grafting-of preparation
Phenylalanine-grafting-tryptophan carries out cytotoxicity inspection using Alamar Blue cell Proliferation/citotoxicity detection kit
Experiment is surveyed, when concentration is less than 0.3mg/mL, smooth muscle cell survival rate is 80% or more.
Poly-aspartate-grafting-phenylalanine-grafting-valine of preparation is 1.0mg/mL with concentration, is 4.5- in pH
Between 7.4 when self assembly, when pH is greater than 4.9, solution becomes clear solution from emulsion.Poly-aspartate-grafting-of preparation
Phenylalanine-grafting-valine carries out cytotoxicity inspection using Alamar Blue cell Proliferation/citotoxicity detection kit
Experiment is surveyed, when concentration is less than 1.3mg/mL, smooth muscle cell survival rate is 80% or more.
Poly-aspartate-grafting-phenylalanine-grafting-isoleucine of preparation is 1.0mg/mL with concentration, is in pH
Between 4.6-7.4 when self assembly, when pH is greater than 4.8, solution becomes clear solution from emulsion.The poly-aspartate-of preparation
Grafting-phenylalanine-grafting-isoleucine using AB reagent carry out cytotoxicity test experience, when concentration be less than 1.7mg/mL,
Smooth muscle cell survival rate is 80% or more.
Embodiment 4:
(1) poly-aspartate-grafting-phenylalanine-grafting-benzyloxycarbonyl group lysine preparation
1) by poly-aspartate (molecular weight Mw=92300) (3.0g, the amount of carboxyl substance are 0.026mol) and 1- (3- bis-
Dimethylaminopropyl) -3- ethyl carbodiimide 3.49g, 0.018mol) it is dissolved in the carbonic acid that 200mL mass concentration is 0.54wt%
Hydrogen sodium water solution obtains the solution that poly-aspartate concentration is 1.5wt%, stirs 60min；
2) poly-aspartate carboxyl and L-phenylalanine methyl ester hydrochloride molar ratio 1:1.2 are pressed, L-phenylalanine first is added
Ester hydrochloride (6.73g, 0.031mol) aqueous solution is in room temperature reaction 72h；
3) 100mL concentration is added is the ethanol solution alkali cleaning of 4.5wt% sodium hydroxide, is stirred overnight at room temperature；
4) solution is fitted into the bag filter of molecular cut off 1kDa, dialyses 4 days, is purified, freeze-drying is gathered afterwards for 24 hours
Aspartic acid-grafting-phenylalanine, phenylalanine grafting rate is 51.5%, as structural formula is as follows:
5) using poly-aspartate-grafting-phenylalanine of step 4) as raw material, in the 250mL that magnetic stir bar is housed
In single-necked flask, it is added poly-aspartate-grafting-phenylalanine (1.5g, the amount 0.0079mol of carboxyl substance), 1- (3- diformazan
Base aminopropyl) -3- ethyl carbodiimide (1.5g, 0.0079mol), 150mL sodium bicarbonate aqueous solution (0.53wt%) obtains
Poly-aspartate-grafting-concentration of phenylalanine is the solution of 1.0wt%；60min is activated at room temperature；
6) by poly-aspartate-grafting-phenylalanine carboxyl and hydrophobic amino acid molar ratio 1:1.2, add benzyloxycarbonyl group
Propylhomoserin methyl ester hydrochloride (3.86g, 0.0095mol) aqueous solution, in room temperature the reaction was continued 60h；
7) 80mL concentration is added is the ethanol solution alkali cleaning of 4.5wt% sodium hydroxide, is stirred overnight at room temperature；
8) solution is fitted into the bag filter of molecular cut off 1kDa, is dialysed 4 days, freeze-drying obtains poly-aspartate-afterwards for 24 hours
Grafting-phenylalanine-grafting-benzyloxycarbonyl group lysine, yield is about 64%, and benzyloxycarbonyl group lysine grafting rate is 14.5%,
Structural formula is as follows:
Wherein, x+y=412；X+z=116.
(2) poly-aspartate-grafting-phenylalanine-grafting-tryptophan preparation
1) using poly-aspartate-grafting-phenylalanine of step 4) as raw material, in the 250mL that magnetic stir bar is housed
In single-necked flask, it is added poly-aspartate-grafting-phenylalanine (1.5g, the amount 0.0079mol of carboxyl substance), 1- (3- diformazan
Base aminopropyl) -3- ethyl carbodiimide (1.36g, 0.0071mol), 100mL sodium bicarbonate aqueous solution (0.40wt%) obtains
The solution for being 0.65wt% to poly-aspartate-grafting-concentration of phenylalanine；45min is activated at room temperature；
2) by carboxyl and hydrophobic amino acid molar ratio 1:0.6, add tryptophan methyl ester hydrochloride (1.21g, 0.0047mol) water
Solution, in room temperature the reaction was continued 56h；
3) 60mL concentration is added is the ethanol solution alkali cleaning of 4.8wt% sodium hydroxide, is stirred overnight at room temperature；
4) solution is fitted into the bag filter of molecular cut off 1kDa, is dialysed 4 days, freeze-drying obtains poly-aspartate-afterwards for 24 hours
Grafting-phenylalanine-grafting-benzyloxycarbonyl group lysine, yield is about 64%, and benzyloxycarbonyl group lysine grafting rate is 27.1%,
Structural formula is as follows:
Wherein, x+y=412；X+z=217.
(3) poly-aspartate-grafting-phenylalanine-grafting-valine preparation
1) it regard step 4) product poly-aspartate-grafting-phenylalanine (II) in (1) as raw material, is stirred equipped with magnetic force
In the 250mL single-necked flask for mixing son, poly-aspartate-grafting-phenylalanine (1.5g, the amount of carboxyl substance is added
0.0079mol), 1- (3- dimethylaminopropyl) -3- ethyl carbodiimide (1.51g, 0.0079mol), 100mL sodium bicarbonate
Aqueous solution (0.50wt%) obtains the solution that poly-aspartate-grafting-concentration of phenylalanine is 1.0wt%；It activates at room temperature
54min；
2) by poly-aspartate-grafting-phenylalanine carboxyl and hydrophobic amino acid molar ratio 1:0.3, add valine first
Ester hydrochloride (0.79g, 0.0047mol) aqueous solution, in room temperature the reaction was continued 60h；
3) 50mL concentration is added is the ethanol solution alkali cleaning of 3.0wt% sodium hydroxide, is stirred overnight at room temperature；
4) solution is fitted into the bag filter of molecular cut off 1kDa, is dialysed 3 days, freeze-drying obtains poly-aspartate-afterwards for 24 hours
Grafting-phenylalanine-grafting-valine, yield is about 71%, and valine grafting rate is 26.1%, and structural formula is as follows:
X+y=412；X+z=209
(4) poly-aspartate-grafting-phenylalanine-grafting-isoleucine preparation
1) it regard step 4) product poly-aspartate-grafting-phenylalanine (II) in (1) as raw material, is stirred equipped with magnetic force
In the 250mL single-necked flask for mixing son, poly-aspartate-grafting-phenylalanine (0.90g, the amount of carboxyl substance is added
0.0056mol), 1- (3- dimethylaminopropyl) -3- ethyl carbodiimide (0.91g, 0.0048mol), 100mL sodium bicarbonate
Aqueous solution (0.35wt%) obtains the solution that poly-aspartate-grafting-concentration of phenylalanine is 0.9wt%；It activates at room temperature
60min；
2) by poly-aspartate-grafting-phenylalanine carboxyl and hydrophobic amino acid molar ratio 1:0.9, add isoleucine
Methyl ester hydrochloride (0.92g, 0.0050mol) aqueous solution, in room temperature the reaction was continued 72h；
3) 80mL concentration is added is the ethanol solution alkali cleaning of 4.0wt% sodium hydroxide, is stirred overnight at room temperature；
4) solution is fitted into the bag filter of molecular cut off 1kDa, is dialysed 3 days, freeze-drying obtains poly-aspartate-afterwards for 24 hours
Grafting-phenylalanine-grafting-isoleucine, yield is about 76%, and isoleucine grafting rate is 29.5%, and structural formula is as follows:
X+y=412；X+z=236
(5) performance test
Poly-aspartate-grafting-phenylalanine-grafting-benzyloxycarbonyl group lysine of preparation has pH responsiveness, is in pH
Clear solution when 7.4, polymer molecular chain are extended position, and when pH is less than 5.7, solution becomes emulsion, polymer aggregational shape
At particle；Cytotoxicity experiment is carried out using Alamar Blue kit, when polymer concentration is less than 0.1mg/mL, to smooth
The survival rate of myocyte all 80% or more, shows poly-aspartate-grafting-phenylalanine-grafting-benzyloxycarbonyl group lysine tool
There is good cell compatibility.
Poly-aspartate-grafting-phenylalanine-grafting-tryptophan of preparation is 1.0mg/mL with concentration, is 4.5- in pH
Between 7.4 when self assembly, when pH is greater than 5.1, solution becomes clear solution from emulsion.Poly-aspartate-grafting-of preparation
Phenylalanine-grafting-tryptophan carries out cytotoxicity inspection using Alamar Blue cell Proliferation/citotoxicity detection kit
Experiment is surveyed, when concentration is less than 0.4mg/mL, smooth muscle cell survival rate is 80% or more.
Poly-aspartate-grafting-phenylalanine-grafting-valine of preparation is 1.0mg/mL with concentration, is 4.4- in pH
Between 7.4 when self assembly, when pH is greater than 4.5, solution becomes clear solution from emulsion.Poly-aspartate-grafting-of preparation
Phenylalanine-grafting-valine carries out cytotoxicity inspection using Alamar Blue cell Proliferation/citotoxicity detection kit
Experiment is surveyed, when concentration is less than 1.6mg/mL, smooth muscle cell survival rate is 80% or more.
Poly-aspartate-grafting-phenylalanine-grafting-isoleucine of preparation is 1.0mg/mL with concentration, is in pH
Between 4.6~7.4 when self assembly, when pH is greater than 4.8, solution becomes clear solution from emulsion.The poly-aspartate-of preparation
Grafting-phenylalanine-grafting-isoleucine using AB reagent carry out cytotoxicity test experience, when concentration be less than 2.1mg/mL,
Smooth muscle cell survival rate is 80% or more.
Illustrative description has been done to the present invention above, it should explanation, the case where not departing from core of the invention
Under, any simple deformation, modification or other skilled in the art can not spend the equivalent replacement of creative work,
Fall into protection scope of the present invention.
Claims (8)
1. a kind of pH responsiveness poly-aspartate of grafted hydrophobic acidic amino acid, it is characterised in that structural formula are as follows:
In formula (I), R NεBenzyloxycarbonyl group lysine or tryptophan or valine or isoleucine；N=39~800；
X >=0, y >=0, z >=0 and x, y and z cannot be 0 simultaneously；n>x+y>0,n>x+y+z>0.
2. the preparation method of the pH responsiveness poly-aspartate of grafted hydrophobic acidic amino acid as described in claim 1, feature
It is: using poly-aspartate-grafting-phenylalanine and with hydrophobic amino acid as raw material, by coupling reaction and alkali cleaning, is produced
Object.
3. method according to claim 2, it is characterized in that hydrophobic amino acid includes NεBenzyloxycarbonyl group lysine benzyl ester hydrochloric acid
Salt, tryptophan methyl ester, valine methyl ester or Isoleucine methyl ester.
4. method according to claim 2, it is characterized in that poly-aspartate-grafting-phenylalanine preparation method is: with poly-
Aspartic acid and L-phenylalanine methyl ester hydrochloride are raw material, pass through 1- (3- dimethylaminopropyl) -3- ethyl carbodiimide
It is catalyzed after reacting, the poly-aspartate-grafting-phenylalanine being prepared through alkali cleaning.
5. method according to claim 2, it is characterized in that the carboxyl of poly-aspartate rubs with what L-phenylalanine methyl esters fed intake
You are than being 1:0.6~1.2；The molar ratio of poly-aspartate carboxyl and 1- (3- dimethylaminopropyl) -3- ethyl carbodiimide
For 1:0.5~1.0.
6. method according to claim 2, it is characterized in that poly-aspartate-grafting-phenylalanine carboxyl and hydrophobic amino
The molar ratio that acid methyl ester hydrochloride salt feeds intake is 1:0.5~1.2；Wherein hydrophobic amino acid methyl ester hydrochloride connects with poly-aspartate-
Branch-phenylalanine carries out reacting to obtain formula (I) structure.
7. method as claimed in claim 6, characterization step is as follows:
1) it takes water as a solvent, configuration quality concentration is the sodium bicarbonate aqueous solution of 0.21~0.63wt%, then by poly- asparagus fern ammonia
The carboxyl-content and 1- (3- dimethylaminopropyl) -3- ethyl carbodiimide of acid are dissolved in carbonic acid according to molar ratio 1:0.5~1.0
In hydrogen sodium water solution, the solution that poly-aspartate concentration is 1~2wt% is obtained, 30~60min is stirred；
2) poly-aspartate carboxyl and L-phenylalanine methyl ester hydrochloride molar ratio 1:0.6~1.2 are pressed, L-phenylalanine first is added
Ester hydrochloride aqueous solution is reacting at room temperature 48~72h；
3) concentration is added is the ethanol solution alkali cleaning of 2.0~5.0wt% sodium hydroxide, is stirred overnight at room temperature, wherein sodium hydroxide
The volume fraction of ethanol solution relative response system is 50~80%；
4) solution is fitted into the bag filter of 0.5~1kDa molecular cut off, with deionized water dialysis 3~5 days, is purified,
Poly-aspartate-grafting-phenylalanine is obtained after freeze-drying, such as formula (II).
T is poly-aspartate-grafting-phenylalanine number of repeat unit, x+y=t in formula (II)；n>t>0；N=39~800.
5) regard the poly-aspartate of step 4)-grafting-phenylalanine (II) as raw material, by the carboxyl-content of poly-aspartate and
1- (3- dimethylaminopropyl) -3- ethyl carbodiimide 1:0.5~1.0 in molar ratio；Being dissolved in sodium bicarbonate mass concentration is
In the sodium bicarbonate aqueous solution of 0.21~0.63wt%, obtain poly-aspartate-grafting-concentration of phenylalanine be 0.5~
The solution of 1.0wt%；30~60min is activated at room temperature；
6) by poly-aspartate-grafting-phenylalanine carboxyl and hydrophobic amino acid molar ratio 1:0.5~1.2, add hydrophobic amino
Acid methyl ester hydrochloride salt (NεBenzyloxycarbonyl group lysine methyl ester hydrochloride, tryptophan methyl ester hydrochloride, valine methyl ester hydrochloride or
Isoleucine methyl ester hydrochloride) aqueous solution, in room temperature the reaction was continued 48~72h；
7) concentration is added is the ethanol solution alkali cleaning of 2.0~5.0wt% sodium hydroxide, is stirred overnight at room temperature, wherein sodium hydroxide
The volume fraction of ethanol solution relative response system is 50~80%；
8) it uses deionized water dialysis solution 3~5 days, obtains the poly-aspartate of grafted hydrophobic acidic amino acid after 24~48h is lyophilized,
Such as formula (I).
8. the poly-aspartate of the grafted hydrophobic acidic amino acid of claim 1 has pH responsiveness, pharmaceutical carrier material can be used as
Material application.
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| CN113087903A (en) * | 2021-03-17 | 2021-07-09 | 西安交通大学 | High-temperature-resistant modified polyaspartic acid scale inhibitor and preparation method and use method thereof |
| CN113307863A (en) * | 2021-05-25 | 2021-08-27 | 华南农业大学 | Preparation method and application of polyaspartic acid and salt antibody thereof |
| CN113321808A (en) * | 2021-06-16 | 2021-08-31 | 苏州美瑞姿生物科技有限公司 | Multifunctional polyaspartic acid derivative and preparation process thereof |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113087903A (en) * | 2021-03-17 | 2021-07-09 | 西安交通大学 | High-temperature-resistant modified polyaspartic acid scale inhibitor and preparation method and use method thereof |
| CN113087903B (en) * | 2021-03-17 | 2022-05-20 | 西安交通大学 | High-temperature-resistant modified polyaspartic acid scale inhibitor and preparation method and use method thereof |
| CN113307863A (en) * | 2021-05-25 | 2021-08-27 | 华南农业大学 | Preparation method and application of polyaspartic acid and salt antibody thereof |
| CN113307863B (en) * | 2021-05-25 | 2022-12-16 | 华南农业大学 | Preparation method and application of polyaspartic acid and salt antibody thereof |
| CN113321808A (en) * | 2021-06-16 | 2021-08-31 | 苏州美瑞姿生物科技有限公司 | Multifunctional polyaspartic acid derivative and preparation process thereof |
| CN113321808B (en) * | 2021-06-16 | 2023-03-07 | 苏州美瑞姿生物科技有限公司 | Multifunctional polyaspartic acid derivative and preparation process thereof |
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