CN109369801A - A kind of preparation method of extremely light fishlike smell collagen peptide - Google Patents
A kind of preparation method of extremely light fishlike smell collagen peptide Download PDFInfo
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- CN109369801A CN109369801A CN201811460290.2A CN201811460290A CN109369801A CN 109369801 A CN109369801 A CN 109369801A CN 201811460290 A CN201811460290 A CN 201811460290A CN 109369801 A CN109369801 A CN 109369801A
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- collagen peptide
- extremely light
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- fish scale
- fishlike smell
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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- Meat, Egg Or Seafood Products (AREA)
Abstract
The present invention relates to a kind of preparation methods of extremely light fishlike smell collagen peptide.Have two step deodorization steps to fish scale: first step deodorization uses diluted green onion, ginger and garlic cooking wine, and second step deodorization uses lemon juice.This method carries out deodorization by the way that the green onion, ginger and garlic cooking wine bought in the market is added in preceding processing, to fish scale；It is added when then passing through post-processing in a small amount of lemon juice and alkaline fishy smell substance, has the function that fishlike smell can be greatly reduced.
Description
Technical field
The present invention relates to the preparation of collagen peptide, in particular to a kind of extremely light fishlike smell collagen peptide
Preparation.
Background technique
The universal appearance of collagen currently produced on the market is more yellow, there is heavier fishlike smell.Influence the receiving of consumer
Degree also above brings certain obstruction in application.Fishy smell in fish scale collagen is mainly to produce in itself and enzymolysis process
Raw substance, such as aldehyde material, Ester etc., trimethylamine oxide etc., existing deodorization technology pass through the high skills such as nanofiltration membrane
The separation of art means, invests larger, general factory is difficult to receive in actual production.
Summary of the invention
The present invention in view of the foregoing drawbacks, and it is an object of the present invention to provide a kind of rational technology, can effectively to fish scale fishy smell at
A kind of preparation method of extremely light fishlike smell collagen peptide of reason.
Thus the technical solution adopted by the present invention is that；A kind of preparation method of extremely light fishlike smell collagen peptide is right
Fish scale has two step deodorization steps: first step deodorization uses diluted green onion, ginger and garlic cooking wine, and second step deodorization uses lemon juice.
Pass through alkali process cleaning by degreasing before first step deodorization step.
Enzymolysis process operation is carried out between first step and second step, and the lemon juice is added after enzymatic hydrolysis inactivation.
The green onion, ginger and garlic cooking wine and water according to 1:5 ~ 10 times dilution proportion.
The feed weight ratio of diluted green onion, ginger and garlic cooking wine and fish scale is 1 ~ 2:1, and the fish scale is impregnated into diluted green onion, ginger and garlic
Among cooking wine, soaking time is 60 ~ 150 minutes.
The weight for pressing fish scale, the lemon juice of 0.5 ~ 1% weight of addition in enzymolysis liquid, stirring at normal temperature 30 ~ 60 minutes.
With lye to fish scale degreasing, with EDTA-2Na to fish scale decalcification, with diluted green onion, ginger and garlic cooking wine to fish scale deodorant, then
It is digested, is inactivated, lemon juice neutralizes, and filters, and concentration, spray drying obtains the collagen peptide of extremely light fishy smell.
Active carbon is added simultaneously when lemon juice is added.
Cleaning by degreasing is carried out using sodium carbonate liquor.
The invention has the advantages that the present invention is inspired by daily life, by using diluted green onion ginger before fish scale digests
Garlic cooking wine impregnates, and on the one hand decomposes fishy smell substance by functional component in green onion, ginger and garlic cooking wine；On the other hand, green onion, ginger and garlic cooking wine may be used also
To dissolve fishy smell organic chemicals, achieve the effect that remove fishy smell jointly；Meanwhile in the enzyme-deactivating later period, by being added on a small quantity
Lemon juice, a small amount of alkaline fishy smell substance generated when neutralizing enzymatic hydrolysis；By active carbon decoloration deodorization, finally make the product of product
Matter gets a promotion；This technique does not add extras, and easy to operate, cost of material also relative moderate, is suitable for industrial metaplasia
Produce application.
Specific embodiment
The present invention is the fish scale that will be cleaned up after degreasing decalcification, according to fish scale: 5 ~ 10 times of diluted green onion, ginger and garlic cooking wine weights
The ratio measured than 1:1 ~ 2 is uniformly mixed, and soak at room temperature is rinsed well after 60 ~ 150 minutes.According to normal enzymatic hydrolysis technological operation, enzyme
After inactivation, 0.5% ~ 1% lemon juice is added into enzymolysis liquid, active carbon decoloration deodorization filters, and is concentrated, and spray drying is made extremely light
The collagen peptide of fishlike smell.
Embodiment 1:
(1) commercially available green onion, ginger and garlic cooking wine is diluted to 5 times.
(2) degreasing: the fresh fish scale 0.15mol/L soaking in sodium carbonate solution of 30 times of weight is slowly stirred 6 hours,
It is rinsed with water to neutrality, filtering.
(3) decalcification: with the fish scale after the EDTA-2Na solution soak degreasing of the 5g/L of 30 times of weight, being slowly stirred 6h, punching
It is washed till neutrality, is filtered.
(4) deodorant: the dilution green onion, ginger and garlic cooking wine measured with 1 times impregnates fish scale after decalcification, is slowly stirred 2h, rinses to neutrality,
The fish scale that must be handled well.
(5) water of 20 times of amounts is added, 2% compound protease is added, 50 DEG C digest 6 hours, are warming up to 90 DEG C and keep the temperature 5 points
Clock, enzyme-deactivating.
(6) 0.5% Fresh Lemon juice is added into enzymolysis liquid for the weight for pressing fish scale, and 3% active carbon, 50 DEG C of guarantors are added
Temperature stirring 45 minutes.Filtering.
(7) being concentrated into solid content is 30%, and spray drying obtains product.
(8) product quality, yield is without significant change.The comparison of 10% solution is prepared, fishlike smell removal is obvious.
Embodiment 2:
(1) commercially available green onion, ginger and garlic cooking wine is diluted to 5 times.
(2) degreasing: the fresh fish scale 0.15mol/L soaking in sodium carbonate solution of 30 times of weight is slowly stirred 6 hours,
It is rinsed with water to neutrality, filtering.
(3) decalcification: with the fish scale after the EDTA-2Na solution soak degreasing of the 5g/L of 30 times of weight, being slowly stirred 6h, punching
It is washed till neutrality, is filtered.
(4) deodorant: the dilution green onion, ginger and garlic cooking wine measured with 2 times impregnates fish scale after decalcification, is slowly stirred 2h, rinses to neutrality,
The fish scale that must be handled well.
(5) water of 20 times of amounts is added, 2% compound protease is added, 50 DEG C digest 6 hours, are warming up to 90 DEG C and keep the temperature 5 points
Clock, enzyme-deactivating.
(6) 1.0% Fresh Lemon juice is added into enzymolysis liquid for the weight for pressing fish scale, and 3% active carbon, 50 DEG C of guarantors are added
Temperature stirring 45 minutes.Filtering.
(7) being concentrated into solid content is 30%, and spray drying obtains product.
(8) product quality, yield is without significant change.The comparison of 10% solution is prepared, fishlike smell removal is obvious.
Claims (9)
1. a kind of preparation method of extremely light fishlike smell collagen peptide, which is characterized in that have two step deodorizations step to fish scale
Rapid: first step deodorization uses diluted green onion, ginger and garlic cooking wine, and second step deodorization uses lemon juice.
2. a kind of preparation method of extremely light fishlike smell collagen peptide according to claim 1, which is characterized in that
Pass through alkali process cleaning by degreasing before first step deodorization step.
3. a kind of preparation method of extremely light fishlike smell collagen peptide according to claim 1, which is characterized in that
Enzymolysis process operation is carried out between first step and second step, and the lemon juice is added after enzymatic hydrolysis inactivation.
4. a kind of preparation method of extremely light fishlike smell collagen peptide according to claim 1, which is characterized in that institute
Green onion, ginger and garlic cooking wine and water are stated according to the dilution proportion of 1:5 ~ 10 times.
5. a kind of preparation method of extremely light fishlike smell collagen peptide according to claim 1, which is characterized in that dilute
The feed weight ratio of the green onion, ginger and garlic cooking wine and fish scale released is 1 ~ 2:1, and the fish scale is impregnated among diluted green onion, ginger and garlic cooking wine, is soaked
Steeping the time is 60 ~ 150 minutes.
6. a kind of preparation method of extremely light fishlike smell collagen peptide according to claim 3, which is characterized in that press
The weight of fish scale, the lemon juice of 0.5 ~ 1% weight of addition in enzymolysis liquid, stirring at normal temperature 30 ~ 60 minutes.
7. a kind of preparation method of extremely light fishlike smell collagen peptide according to claim 6, which is characterized in that use
Lye with diluted green onion, ginger and garlic cooking wine to fish scale deodorant, then digests fish scale degreasing with EDTA-2Na to fish scale decalcification,
Inactivation, lemon juice neutralize, and filter, and concentration, spray drying obtains the collagen peptide of extremely light fishy smell.
8. a kind of preparation method of extremely light fishlike smell collagen peptide according to claim 7, which is characterized in that
Active carbon is added when lemon juice is added simultaneously.
9. a kind of preparation method of extremely light fishlike smell collagen peptide according to claim 2, which is characterized in that adopt
Cleaning by degreasing is carried out with sodium carbonate liquor.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811460290.2A CN109369801A (en) | 2018-11-30 | 2018-11-30 | A kind of preparation method of extremely light fishlike smell collagen peptide |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811460290.2A CN109369801A (en) | 2018-11-30 | 2018-11-30 | A kind of preparation method of extremely light fishlike smell collagen peptide |
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| Publication Number | Publication Date |
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| CN109369801A true CN109369801A (en) | 2019-02-22 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201811460290.2A Pending CN109369801A (en) | 2018-11-30 | 2018-11-30 | A kind of preparation method of extremely light fishlike smell collagen peptide |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114668138A (en) * | 2022-03-31 | 2022-06-28 | 青岛蓝色康典海洋生物科技有限公司 | Preparation method and application of fishy smell removed fish scale glue |
-
2018
- 2018-11-30 CN CN201811460290.2A patent/CN109369801A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114668138A (en) * | 2022-03-31 | 2022-06-28 | 青岛蓝色康典海洋生物科技有限公司 | Preparation method and application of fishy smell removed fish scale glue |
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| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20190222 |
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| WD01 | Invention patent application deemed withdrawn after publication |