CN109369234A - A kind of preparation method of composite bacteria biological organic fertilizer - Google Patents

A kind of preparation method of composite bacteria biological organic fertilizer Download PDF

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Publication number
CN109369234A
CN109369234A CN201811167792.6A CN201811167792A CN109369234A CN 109369234 A CN109369234 A CN 109369234A CN 201811167792 A CN201811167792 A CN 201811167792A CN 109369234 A CN109369234 A CN 109369234A
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organic fertilizer
fertilizer
biological organic
soil
bacteria
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CN201811167792.6A
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张云广
张棣信
张浩然
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Shandong Blue Green Source Biotechnology Co Ltd
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Shandong Blue Green Source Biotechnology Co Ltd
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05DINORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D1/00Fertilisers containing potassium

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  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Inorganic Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Fertilizers (AREA)

Abstract

The present invention provides a kind of preparation method of composite bacteria biological organic fertilizer, comprising the following steps: (1) screening of complex microorganism bacterial classification with separate;(2) the key process technology research of complex micro organism fungicide and organic fertilizer inoculation;(3) formulation of the target level of product quality of biological organic fertilizer;(4) industrialization production of biological organic fertilizer.The suitable microorganism of present invention selection, filter out the special microorganism bacterial strain for organic fertilizer, and then it is trained the biological organic fertilizer of high-efficiency environment friendly, it is China's microbial manure manufacturing enterprise, agricultural planting cooperative society and vast farmers provide the microorganism fungus kind for organic matter fermentation, turn waste into wealth, for the sustainable development of China's agricultural, agricultural product quality and safety are promoted, improves planting environment, it realizes environmental benefit, great function will be generated.The project meets the target of national " circular economy " and " low-carbon economy ".The project implementation has significant economic benefit, social benefit and ecological benefits.

Description

A kind of preparation method of composite bacteria biological organic fertilizer
Technical field
The invention belongs to technical field of biological fertilizer, in particular to a kind of preparation method of composite bacteria biological organic fertilizer.
Background technique
The amount of application of China's chemical fertilizer has reached annual 40000 kiloton or more, and mainly based on single chemical fertilizer, such as nitrogenous fertilizer With phosphate fertilizer.Loss of nitrogenous fertilizer is serious, causes river, and lake pollution, the agricultural product nitrate such as vegetables is exceeded, the excessive use of phosphate fertilizer, The accumulation of the heavy metals such as substance and Cd in the soil is write in radiation in phosphorus ore, and will affect the edible safety of agricultural product, while mistake The abuse chemical fertilizer of amount, causes soil hardening, and the content of organic matter is low, and the Soil Loss content of organic matter is 7-10 times of China, root According to data, such as U.S. of developed country, Britain, Japan, which waited, has produced multiple-microorganism bacterial manure, it can be seen that China Fertilization Level far lag behind developed country.In recent years, relevant department advocates applying organic manure energetically, this is disliked to soil property is alleviated Change has certain effect, and the application of organic fertilizer promotes the breeding of edaphon, improves the physics and chemistry of soil to a certain extent Environment, but organic fertilizer must have a large amount of microorganism to participate in decomposing, and not beneficial microorganism fungus kind participates in movable organic fertilizer, It will be water without a source, the end of no sheet.Therefore, researching and developing new and effective that of fertilizer quality microbial bacterial agent is to solve the problems, such as this Key.
Biological organic fertilizer refers to microorganisms with specific functions and mainly with plant and animal residues such as feces of livestock and poultry, agricultural crop straw etc. For source and one kind for being combined through harmless treatment, decomposed organic materials has both microbial manure and organic fertilizer effect Fertilizer.Divide by the performance and type difference of its microorganism: the relatively simple biological organic fertilizer of function such as phosphobacteria biological organic Fertilizer, fixed nitrogen bacteria organic fertilizer and potassium decomposing bacteria organic fertilizer.Its action principle is increased in soil using its vital movement Nitrogen or available phosphorus, the content of potassium, or the substance that some crops in soil cannot directly be utilized, being converted to can be absorbed Nutriment, or improve the production excitor substance of crop, or inhibit the activity of phytopathogen, to increase soil fertility, Improve the nutritional condition of crop, improves crop yield.
Biological organic fertilizer produces organic fertilizer using the feces of livestock and poultry of farm, not only solves farm's pollution and cultivation peace Full problem greatly improves life and the working environment of numerous practitioners, also becomes one new growth engines of farm. Two or more mutually not biological organic fertilizer made of the microorganism fungus kind of antagonism is added, generally has type complete, compatibility Rationally, the advantages that functional, high financial profit, in crops using upper, to crop yield is improved, enhancing crops are resistance to Inverse property, disease and insect resistance, improves product quality and improvement soil property has positive effect.
Summary of the invention
In order to solve the above technical problem, the present invention provides a kind of preparation methods of composite bacteria biological organic fertilizer.This hair The suitable microorganism of bright selection, filters out the special microorganism bacterial strain for organic fertilizer, and then be trained the biology of high-efficiency environment friendly Organic fertilizer, is China's microbial manure manufacturing enterprise, and agricultural planting cooperative society and vast farmers are provided for organic matter fermentation Microorganism fungus kind is turned waste into wealth, and is the sustainable development of China's agricultural, is promoted agricultural product quality and safety, improves planting environment, It realizes environmental benefit, great function will be generated.The project meets the target of national " circular economy " and " low-carbon economy ".The project Implementation have significant economic benefit, social benefit and ecological benefits.
The present invention is achieved through the following technical solutions, and the present invention provides a kind of preparation of composite bacteria biological organic fertilizer Method, comprising the following steps:
(1) screening of complex microorganism bacterial classification with separate: separate effective bacterium from soil and plant rhizosphere, strain carried out after screening Optimum organization;
(2) complex micro organism fungicide and organic fertilizer inoculation key process technology research: based on workers and peasants produce in waste and House refuse carries out processing organic carrier by adding inorganic fertilizer, is inoculated with to effective bacterium and carrier, inquires into different fertilizer Organic matter, nitrogen, phosphorus element, potassium and microelement match on the active influence of composite bacteria, target be intended to determine be suitable for not With the formula of the organic fertilizer of strain;
(3) formulation of the target level of product quality of biological organic fertilizer: by the field trial of large area, to biological organic fertilizer and tradition Fertilizer compares and analyzes, and determines product standard index according to crop yield and quality;
(4) industrialization production of biological organic fertilizer.
Preferably, the screening of step (1) complex microorganism bacterial classification with separate, specifically include:
Soil sampling: from red soil, yellow earth, yellowish soil, drab soil, brown earth separates sulfide-oxidizing enzyme in rice soil and Shajiang black soil, solves Phosphorus bacterium, amonifying bacteria, nitrogen-fixing bacteria, rhizobium, actinomyces, potassium solubilizing bacteria etc..
Bacteria selection: through plate culture, shaking table culture, purification, then strain calibrating carries out rejuvenation, as inoculation bacterium Kind.
The microbial bacterial agent exploitation of multiple and different function strain compositions: by culture experiment, different strain is got clear in difference Coordinated growth ability under environmental condition, growth rhythm and antagonism, determine best antimicrobial composition.
Preferably, the key process technology research of step (2) composite microbial bacteria and organic fertilizer inoculation, specifically includes:
The processing of carrier: using in industrial and agricultural production waste and feces of livestock and poultry, stalk, house refuse etc. as organic matter material, Through high temperature harmless treatment, according to the organic matter nutrient content after processing, be added suitable inorganic fertilizer support different organic matters, The organic fertilizer of nutrient content, as biological organic fertilizer carrier.
The activity analysis of microbe inoculation: being inoculated with efficient bacterium on carrier, analyzes the pH of carrier, and moisture content saves Method determines suitable technique and method to the active influence of efficient bacterium.
Preferably, the formulation of step (3) bio-organic fertilizer product quality standard: choose efficient bacterium quantity, organic matter, The indexs such as nitrogen, phosphorus element, potassium and microelement, pedo relict nutrient validation amount, crop yield, make biological organic fertilizer Target level of product quality.
Preferably, the industrialization production of step (4) biological organic fertilizer: according to the standard index of product, collect workers and peasants and Waste in life carries out industry processing, batch machining is carried out according to production technology scheme, and establish Demonstration Base, for pushing away Extensively.
Beneficial effects of the present invention:
The suitable microorganism of present invention selection, filters out the special microorganism bacterial strain for organic fertilizer, and then be trained Efficient Ring The biological organic fertilizer of guarantor, is China's microbial manure manufacturing enterprise, and agricultural planting cooperative society and vast farmers are provided for organic The microorganism fungus kind of matter fermentation, turns waste into wealth, and is the sustainable development of China's agricultural, promotes agricultural product quality and safety, improves Planting environment realizes environmental benefit, will generate great function.The project meets the mesh of national " circular economy " and " low-carbon economy " Mark.The project implementation has significant economic benefit, social benefit and ecological benefits.
Specific embodiment
It is noted that following detailed description is all illustrative, it is intended to provide further instruction to the application.Unless another It indicates, all technical and scientific terms used herein has usual with the application person of an ordinary skill in the technical field The identical meanings of understanding.
As background technology part is introduced, biological organic fertilizer produces organic fertilizer using the feces of livestock and poultry of farm, no Address only farm pollution and cultivation safety problem, greatly improve life and the working environment of numerous practitioners, also at For one new growth engines of farm.Adding two or more, mutually biology made of the microorganism fungus kind of antagonism does not have Machine fertilizer, generally has type complete, the advantages that compatibility is reasonable, functional, high financial profit, right in crops using upper Crop yield is improved, enhances crops resistance of reverse, disease and insect resistance, improving product quality and improvement soil property has actively Meaning.
The present invention provides a kind of preparation method of composite bacteria biological organic fertilizer, comprising the following steps:
(1) screening of complex microorganism bacterial classification with separate: separate effective bacterium from soil and plant rhizosphere, strain carried out after screening Optimum organization;
(2) complex micro organism fungicide and organic fertilizer inoculation key process technology research: based on workers and peasants produce in waste and House refuse carries out processing organic carrier by adding inorganic fertilizer, is inoculated with to effective bacterium and carrier, inquires into different fertilizer Organic matter, nitrogen, phosphorus element, potassium and microelement match on the active influence of composite bacteria, target be intended to determine be suitable for not With the formula of the organic fertilizer of strain;
(3) formulation of the target level of product quality of biological organic fertilizer: by the field trial of large area, to biological organic fertilizer and tradition Fertilizer compares and analyzes, and determines product standard index according to crop yield and quality;
(4) industrialization production of biological organic fertilizer.
The screening of step (1) complex microorganism bacterial classification with separate, specifically include:
Soil sampling: from red soil, yellow earth, yellowish soil, drab soil, brown earth separates sulfide-oxidizing enzyme in rice soil and Shajiang black soil, solves Phosphorus bacterium, amonifying bacteria, nitrogen-fixing bacteria, rhizobium, actinomyces, potassium solubilizing bacteria etc..
Bacteria selection: through plate culture, shaking table culture, purification, then strain calibrating carries out rejuvenation, as inoculation bacterium Kind.
The microbial bacterial agent exploitation of multiple and different function strain compositions: by culture experiment, different strain is got clear in difference Coordinated growth ability under environmental condition, growth rhythm and antagonism, determine best antimicrobial composition.
The key process technology research of step (2) composite microbial bacteria and organic fertilizer inoculation, specifically includes:
The processing of carrier: using in industrial and agricultural production waste and feces of livestock and poultry, stalk, house refuse etc. as organic matter material, Through high temperature harmless treatment, according to the organic matter nutrient content after processing, be added suitable inorganic fertilizer support different organic matters, The organic fertilizer of nutrient content, as biological organic fertilizer carrier.
The activity analysis of microbe inoculation: being inoculated with efficient bacterium on carrier, analyzes the pH of carrier, and moisture content saves Method determines suitable technique and method to the active influence of efficient bacterium.
The formulation of step (3) bio-organic fertilizer product quality standard: efficient bacterium quantity, organic matter, nitrogen, phosphorus are chosen The indexs such as plain, potassium and microelement, pedo relict nutrient validation amount, crop yield, make bio-organic fertilizer product matter Amount standard.
The industrialization production of step (4) biological organic fertilizer: it according to the standard index of product, collects useless in workers and peasants and life Object carries out industry processing, batch machining is carried out according to production technology scheme, and establish Demonstration Base, for promoting.
Embodiment 1
The application experiment of biological organic fertilizer
1. influence of the microbial-bacterial fertilizer to cucumber growth situation and yield
One piece of experimental plot is selected to apply biological organic fertilizer with field, experimental plot cucumber is compareed, common chemical fertilizer is applied in control field.
2. application test of the microbial-bacterial fertilizer on radish
In order to study application microbial-bacterial fertilizer to radish physiological property, the influence of yield and quality and planting environment is arranged at chemical fertilizer Reason, organic fertilizer processing are handled with microbial-bacterial fertilizer, 3 kinds of different fertilizer types.
3. preliminary test of the microbial-bacterial fertilizer in tomato in plastic greenhouse
The effect of in order to verify microbial manure, applies microbial-bacterial fertilizer to the tomato in plastic greenhouse of Shouguang ecological park.
This project aims to solve the problem that house refuse, town dweller's excrement, feces of livestock and poultry, agricultural crop straw pollution, and deodorizing is killed Bacterium removes moisture, decomposing organic matter, so that it becomes plant is easy the nutritional ingredient absorbed.With house refuse, town dweller's excrement, Feces of livestock and poultry, agricultural crop straw are efficient biofertilizer carrier.Efficient bacterium in soil is screened, is inoculated with after combination, determines different groups The carrier components content and processing technology of combined bacteria kind analyze the biological effect and environmental effect of product, establish Demonstration Base, carry out Industrialization production improves the utilization rate of pedo relict element the purpose is to increase soil trace element, and it is several to reduce environmental pollution Rate, and reduce cost.
Certainly, above description is also not limited to the example above, technical characteristic of the present invention without description can by or It is realized using the prior art, details are not described herein;It is not to this that the above examples are only used to illustrate the technical scheme of the present invention The limitation of invention, describes the invention in detail referring to preferred embodiment, and those skilled in the art should Understand, the variations, modifications, additions or substitutions that those skilled in the art are made within the essential scope of the present invention Without departure from spirit of the invention, it also should belong to claims of the invention.

Claims (5)

1. a kind of preparation method of composite bacteria biological organic fertilizer, it is characterised in that: the following steps are included:
(1) screening of complex microorganism bacterial classification with separate: separate effective bacterium from soil and plant rhizosphere, strain carried out after screening Optimum organization;
(2) complex micro organism fungicide and organic fertilizer inoculation key process technology research: based on workers and peasants produce in waste and House refuse carries out processing organic carrier by adding inorganic fertilizer, is inoculated with to effective bacterium and carrier, inquires into different fertilizer Organic matter, nitrogen, phosphorus element, potassium and microelement match on the active influence of composite bacteria, target be intended to determine be suitable for not With the formula of the organic fertilizer of strain;
(3) formulation of the target level of product quality of biological organic fertilizer: by the field trial of large area, to biological organic fertilizer and tradition Fertilizer compares and analyzes, and determines product standard index according to crop yield and quality;
(4) industrialization production of biological organic fertilizer.
2. a kind of preparation method of composite bacteria biological organic fertilizer according to claim 1, it is characterised in that: step (1) The screening of complex microorganism bacterial classification with separate, specifically include:
(1) soil sampling: from red soil, yellow earth, yellowish soil, drab soil, brown earth, separation vulcanization is thin in rice soil and Shajiang black soil Bacterium, phosphate solubilizing bacteria, amonifying bacteria, nitrogen-fixing bacteria, rhizobium, actinomyces, potassium solubilizing bacteria etc.;
(2) bacteria selection: through plate culture, shaking table culture, purification, then strain calibrating carries out rejuvenation, as inoculation bacterium Kind;
(3) the microbial bacterial agent exploitation of multiple and different function strain compositions: by culture experiment, different strain is got clear in different rings Coordinated growth ability under the conditions of border, growth rhythm and antagonism, determine best antimicrobial composition.
3. a kind of preparation method of composite bacteria biological organic fertilizer according to claim 1, it is characterised in that: step (2) The key process technology research of composite microbial bacteria and organic fertilizer inoculation, specifically includes:
(1) processing of carrier: using in industrial and agricultural production waste and feces of livestock and poultry, stalk, house refuse etc. as organic matter original It is different organic that suitable inorganic fertilizer support is added according to the organic matter nutrient content after processing through high temperature harmless treatment in material The organic fertilizer of matter, nutrient content, as biological organic fertilizer carrier;
(2) activity analysis of microbe inoculation: being inoculated with efficient bacterium on carrier, analyzes the pH of carrier, moisture content, preservation side Method determines suitable technique and method to the active influence of efficient bacterium.
4. a kind of preparation method of composite bacteria biological organic fertilizer according to claim 1, it is characterised in that: step (3) The formulation of bio-organic fertilizer product quality standard: efficient bacterium quantity, organic matter, nitrogen, phosphorus element, potassium and micro member are chosen The indexs such as element, pedo relict nutrient validation amount, crop yield, make bio-organic fertilizer product quality standard.
5. a kind of preparation method of composite bacteria biological organic fertilizer according to claim 1, it is characterised in that: step (4) The industrialization production of biological organic fertilizer: according to the standard index of product, the waste collected in workers and peasants and life carries out industry processing, Batch machining is carried out according to production technology scheme, and establishes Demonstration Base, for promoting.
CN201811167792.6A 2018-10-08 2018-10-08 A kind of preparation method of composite bacteria biological organic fertilizer Pending CN109369234A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112694996A (en) * 2021-01-13 2021-04-23 南昌大学 Method for artificially constructing plant growth-promoting complex functional flora

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CN101648827A (en) * 2009-09-04 2010-02-17 郭凯 Fermented biological organic fertilizer and processing method thereof
CN102584370A (en) * 2012-02-29 2012-07-18 王宝申 Technology for preparing bio-fertilizer from garbage

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1285401A (en) * 1999-08-20 2001-02-28 中国华联国际服务联合公司 Azotobacteria and azotogen, azotogen composition and application thereof
CN1600750A (en) * 2003-09-26 2005-03-30 中国科学院沈阳应用生态研究所 Method for producing multifunctional organic fertilizer of microbe
CN101648827A (en) * 2009-09-04 2010-02-17 郭凯 Fermented biological organic fertilizer and processing method thereof
CN102584370A (en) * 2012-02-29 2012-07-18 王宝申 Technology for preparing bio-fertilizer from garbage

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112694996A (en) * 2021-01-13 2021-04-23 南昌大学 Method for artificially constructing plant growth-promoting complex functional flora

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