CN109364047A - 四氢姜黄素在制备治疗白癜风药物或化妆品中的应用 - Google Patents

四氢姜黄素在制备治疗白癜风药物或化妆品中的应用 Download PDF

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Publication number
CN109364047A
CN109364047A CN201811110020.9A CN201811110020A CN109364047A CN 109364047 A CN109364047 A CN 109364047A CN 201811110020 A CN201811110020 A CN 201811110020A CN 109364047 A CN109364047 A CN 109364047A
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China
Prior art keywords
cosmetics
tetrahydro curcumin
application
preparation treatment
melanin
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CN201811110020.9A
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Inventor
王艳
靳佳慧
杨珍
郭虹
庄朋伟
郭毅群
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Tianjin University of Traditional Chinese Medicine
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Tianjin University of Traditional Chinese Medicine
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Priority to CN201811110020.9A priority Critical patent/CN109364047A/zh
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/12—Ketones
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
    • A61K8/35—Ketones, e.g. benzophenone
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin

Abstract

本发明公开了四氢姜黄素在制备治疗白癜风药物或化妆品中的应用。实验证明，四氢姜黄素在避光条件下外用，可促进黑色素的生成，具有治疗白癜风的作用。

Description

四氢姜黄素在制备治疗白癜风药物或化妆品中的应用
技术领域
本发明涉及一种化合物的新用途，具体地涉及一种四氢姜黄素在制备治疗白癜风药物或化妆品中的应用。
背景技术
黑色素的产生是普遍的，是广泛分布于整个动物和植物界的吲哚聚合物，也可由微生物合成。黑色素有许多生物学功能，例如其可通过吸收宽范围的电磁波谱以防止光致损伤来表现对紫外光的抵抗力。因此，它被广泛用于化妆品和光防护乳膏中。
皮肤颜色主要由皮肤中黑色素的含量决定，黑色素由黑素细胞分泌，黑素细胞位于表皮的基底层，是皮肤中黑色素合成的场所。白癜风是一种后天性黑素细胞特发性损伤和功能异常而色素脱失的全身性皮肤病,主要表现为局部或泛发的色素脱失。作为毁容性皮肤病,白癜风不仅严重影响患者的心理健康与社交活动,而且还给患者及其家庭带来不小的经济压力,因此积极探寻能治疗白癜风的天然药物具有重要意义。
四氢姜黄素(Tetrahydrocurcumin，THC)是一种多酚类化合物，作为姜黄素的主要代谢产物，其具有与姜黄素几乎相似的生理和药理学性质，但THC在临床上比姜黄素具有更有利的性质。近年来，由于其广泛的生理活性、丰富的来源和低廉的价格，THC受到的关注越来越多。目前关于THC的报道多说明其可通过抑制酪氨酸酶的活性来减少黑色素的生成，用做皮肤美白剂，尚未有将THC用于促进表皮黑素细胞的黑色素生成的报道。
发明内容
本发明的目的是提供四氢姜黄素在制备治疗白癜风药物或化妆品中的应用。
本发明的技术方案概述如下：
四氢姜黄素在制备治疗白癜风药物或化妆品中的应用。
实验证明，四氢姜黄素在避光条件下外用，可促进黑色素的生成，具有治疗白癜风的作用。
附图说明
图1为未照射UVB，不同浓度的THC对PIG1细胞中黑色素含量的影响(与对照组比较，1)表示：P＜0.05，有统计学意义)。
具体实施方式
下面通过具体实施例对本发明作进一步的说明。
实施例1
1仪器与材料(细胞和试剂)
1.1实验仪器
实验仪器见表1
表1实验所用仪器一览表
1.2实验材料
实验所用材料见表2
表2实验所用材料一览表
表2所示的实验所用试剂，仅是举例，并不对本发明作任何限定。
2样品制备
完全M254培养基的配制：5％胎牛血清的M254培养基，加入HMGS-2，体积含量为0.5％(不含抗生素)。
四氢姜黄素溶液的制备：取四氢姜黄素适量，用DMSO(细胞级)溶解制成100μM的母液，取母液0.6、1.2、1.8mL分别加入到2.4、1.8、1.2mL的完全M254培养基中混匀，得到20、40、60μM的四氢姜黄素溶液。
细胞黑色素裂解液制备：取NaOH固体适量，用蒸馏水配制成1M的NaOH溶液45mL，取DMSO5mL，混匀，即用即配。
3实验方法
3.1人表皮黑色素细胞PIG1的培养
人表皮黑色素细胞PIG1(原代正常人皮肤黑素细胞PIG1)置于完全M254培养基中，于含37℃5％CO2恒温培养箱(下简称培养箱)中常规培养。用0.05％的胰酶消化细胞后，计数，接种于6孔板中，每孔个数为2.0×105个，置于培养箱中培养24h。弃掉培养基用PBS溶液(pH＝6.8)清洗2次，弃掉PBS溶液(pH＝6.8)后向对应的6孔板中分别加入3mL的完全M254培养基(对照组)、加入3mL的步骤2配制的四氢姜黄素溶液(实验组)，培养48h后进行后续实验。
3.2黑色素含量的检测
培养结束后，用0.05％的胰酶消化，计数，离心，加入细胞黑色素裂解液100μL，90℃水浴1h，在酶标仪中摇30s于405nm处测定OD值。
4实验结果
见图1
5结果
通过检测对照组和实验组PIG1细胞中的黑色素的含量，结果发现：实验组和对照组相比较，实验组能够显著增加黑色素含量。说明在不给予紫外照射的条件下，四氢姜黄素能够促进黑色素生成。
将本发明涉及到的四氢姜黄素按常规方法，加上药学可接受的辅料，制备成溶液剂、洗剂、搽剂、软膏剂、硬膏剂及贴剂等剂型，做为药物和化妆品，用于由于皮肤和毛囊内的黑素细胞活性降低以致黑色素合成含量降低造成的疾病白癜风的治疗。

Claims (1)

1.四氢姜黄素在制备治疗白癜风药物或化妆品中的应用。
CN201811110020.9A 2018-09-21 2018-09-21 四氢姜黄素在制备治疗白癜风药物或化妆品中的应用 Pending CN109364047A (zh)

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6306383B1 (en) * 1998-09-16 2001-10-23 Wilson T Crandall Method for topical treatment of scars with protein kinase C inhibitors
JP2005112786A (ja) * 2003-10-08 2005-04-28 Nikko Chemical Co Ltd クルクミン誘導体からなるメラニン産生促進剤及びその利用
CN101903034A (zh) * 2007-12-21 2010-12-01 Asac生物技术研究股份公司 提高类姜黄素及其类似物的治疗功效的方法

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6306383B1 (en) * 1998-09-16 2001-10-23 Wilson T Crandall Method for topical treatment of scars with protein kinase C inhibitors
JP2005112786A (ja) * 2003-10-08 2005-04-28 Nikko Chemical Co Ltd クルクミン誘導体からなるメラニン産生促進剤及びその利用
CN101903034A (zh) * 2007-12-21 2010-12-01 Asac生物技术研究股份公司 提高类姜黄素及其类似物的治疗功效的方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
PRAVIT ASAWANONDA等: "Tetrahydrocurcuminoid Cream Plus Targeted Narrowband UVB Phototherapy for Vitiligo: A Preliminary Randomized Controlled Study", 《PHOTOMEDICINE AND LASER SURGERY》 *

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Application publication date: 20190222