CN109358018A - A method of it is traced to the source using infrared spectroscopy and identifies the six fort tea place of production of finished product - Google Patents
A method of it is traced to the source using infrared spectroscopy and identifies the six fort tea place of production of finished product Download PDFInfo
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- 241001122767 Theaceae Species 0.000 title claims abstract description 107
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 41
- 238000000034 method Methods 0.000 title claims abstract description 23
- 238000004566 IR spectroscopy Methods 0.000 title claims description 36
- IOLCXVTUBQKXJR-UHFFFAOYSA-M potassium bromide Chemical compound [K+].[Br-] IOLCXVTUBQKXJR-UHFFFAOYSA-M 0.000 claims abstract description 22
- 239000000843 powder Substances 0.000 claims abstract description 18
- 238000001035 drying Methods 0.000 claims abstract description 17
- 238000002405 diagnostic procedure Methods 0.000 claims abstract description 10
- 238000000227 grinding Methods 0.000 claims abstract description 8
- 238000001914 filtration Methods 0.000 claims abstract description 6
- 238000001228 spectrum Methods 0.000 claims description 49
- 239000000203 mixture Substances 0.000 claims description 15
- 238000005259 measurement Methods 0.000 claims description 6
- 238000012545 processing Methods 0.000 claims description 6
- 238000004364 calculation method Methods 0.000 claims description 4
- 230000001186 cumulative effect Effects 0.000 claims description 4
- 230000003595 spectral effect Effects 0.000 claims description 4
- 238000002156 mixing Methods 0.000 abstract description 4
- 238000007781 pre-processing Methods 0.000 abstract 1
- 239000002994 raw material Substances 0.000 description 6
- 238000002329 infrared spectrum Methods 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 235000009024 Ceanothus sanguineus Nutrition 0.000 description 3
- 240000003553 Leptospermum scoparium Species 0.000 description 3
- 235000015459 Lycium barbarum Nutrition 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 238000004451 qualitative analysis Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 238000002835 absorbance Methods 0.000 description 2
- 229910002092 carbon dioxide Inorganic materials 0.000 description 2
- 239000001569 carbon dioxide Substances 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 238000000513 principal component analysis Methods 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 238000012795 verification Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241000037488 Coccoloba pubescens Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 238000000862 absorption spectrum Methods 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
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- 150000001875 compounds Chemical class 0.000 description 1
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- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
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- 238000012827 research and development Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000010183 spectrum analysis Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
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Abstract
The present invention has studied a kind of method traced to the source using infrared spectrogram and identify the six fort tea place of production of finished product, includes the following steps: that six fort tea samples are smashed it through screen respectively and obtain tea powder to be determined by (1)；(2) using Background Samples are obtained after potassium bromide grinding filtering drying, dress sample tabletting after the tea powder one by one mixing of independent and Background Samples is obtained into tabletting to be detected；(3) tabletting to be detected is put in after being scanned in the sample bin of infrared spectrometer one by one and obtains infrared spectrogram to be determined；(4) infrared spectrogram to be determined is obtained after tabletting to be detected being put in infrared spectrometer scanning；(5) after pre-processing infrared spectrogram, certain wave bands in the characteristic wave of wave band are chosen as variable, the center of mass values in the six fort tea sample places of production to be determined is found out according to Weir this diagnostic method, determines and whether is grown in protection zone.It can effectively identify whether six fort tea tealeaves originate from specified protection zone using method of the invention, have the characteristics that recognition accuracy is high.
Description
Technical field
Trace to the source the present invention relates to one kind and identify tea-leaf producing area field, more specifically more particularly to it is a kind of utilize infrared light
Compose the method for identifying the six fort tea place of production of finished product of tracing to the source.
Background technique
Infrared spectroscopy is the classical way that structure elucidation and confirmation are carried out to pure compound, and infrared qualitative analysis can be by building
Whether the qualitative analysis model of vertical known substance, being prejudged to unknown sample is the substance having been established in qualitative analysis model.
When with middle infrared scan sample, when the component frequency of certain wavelength is identical as the molecular vibrational frequency in sample composition in incident light
RESONANCE ABSORPTION will occur, to form characteristic absorption peak in corresponding spectrum area, will form the true absorption spectrum of sample.To mixing
Object is measured, and various composition will form specific " finger-print " close with blend sample composition, when it is therein at
When point changing, finger-print also changes, and can identify different mixtures with " finger-print ".
In food analysis, due to its various composition complicated composition, identification progress Qualitive test is separated one by one and is then needed
It takes a substantial amount of time and the research and development of technology, it is difficult to apply in actual operation.Using various different mixtures group not
Together, always there is a degree of difference in infrared spectroscopy, the identification that is directly consistent is carried out to mixture.
Six fort tea, usually the selection Wuzhou Cangwu County town Liu Bao protection zone in-group's kind, Guangxi masses' leaf kind machine
Separation, the kind of breeding, the fresh leaf of strain tea tree are raw material, carry out processing and fabricating by specific technique and form.With in recent years
The demand of six fort tea is increasing, and sales volume is become better and better, many Wuzhou Cangwu County town Liu Bao protection zones with for place
Start to plant the tea tree of the kind or strain successively, and is made six fort tea with its fresh leaf.Now six fort tea products in the market
Type is more and more, and for businessman in order to show the quality of its product, much all bragging about its six forts tea is to select Wuzhou Cangwu County six
The fresh leaf for the tea tree planted in the protection zone Bao Zhen is made for raw material, makes one the true and false that can not distinguish its affiliated place of production.Therefore,
Six fort tea place of production methods can effectively be identified by urgently inventing one kind.
Summary of the invention
The purpose of the present invention is to provide a kind of to be traced to the source the method for identifying the six fort tea place of production of finished product using infrared spectroscopy, is utilized
This method can effectively identify whether six fort tea tealeaves originate from specified protection zone, with the high spy of recognition accuracy
Point.
Technical scheme is as follows:
A method of it is traced to the source using infrared spectroscopy and identifies the six fort tea place of production of finished product, included the following steps:
(1) six fort tea samples to be determined are smashed it through into screen respectively and obtain tea powder to be determined；
(2) Background Samples are obtained after grinding filtering drying using the potassium bromide of spectroscopic pure grade；
(3) by tea powder to be determined one by one individually and Background Samples mix in proportion after fill sample tabletting obtain tabletting to be detected；
(4) tabletting to be detected is put in after being scanned in the sample bin of infrared spectrometer one by one and obtains infrared spectrogram to be determined；
(5) after being pre-processed infrared spectrogram to be determined, certain wave bands in the characteristic wave of wave band are chosen as change
Amount finds out the center of mass values in the six fort tea sample places of production to be determined according to Weir this diagnostic method, determines and whether be grown in protection zone
In domain.
Preferably, in the step (1), six fort tea samples to be determined are put into powder in high speed Universal pulverizer
It is broken, smash it through 100 mesh screens.
Preferably, in the step (2), the potassium bromide of the spectroscopic pure grade by grinding is crossed into 200 mesh screen below,
Drying and processing is carried out again, and drying temperature is 120 DEG C, and drying time is 4 hours or more.
Preferably, in the step (3), the mixed weight ratio of tea powder to be determined and Background Samples is 1:100-150.
Preferably, in the step (4), the infrared spectrometer is Fourier infrared spectrograph, spectral region
For 4000-400cm-1, scanning signal is 16 times cumulative, resolution ratio 4cm-1。
Preferably, in the step (5), following steps are specifically divided into:
(5.1) infrared spectroscopy pretreatment figure to be determined is obtained after being pre-processed infrared spectrogram to be determined；
(5.2) obtaining wave band in infrared spectroscopy to be determined pretreatment figure is 3000-2000cm-1、1700-1000cm-1、
850-600cm-1As fingerprint characteristic spectrum, the wave number of some wavelength is obtained respectively in the fingerprint characteristic spectrum, according to
This diagnostic method of Weir finds out the center of mass values in the six fort tea sample places of production to be determined, the calculation formula of center of mass values are as follows:
Y1=-0.185X1-0.333X2+0.32X3+0.45X4+0.016X5+0.477X6+0.016X7+0017X8++
0.016X9-0.008X10+0.05X11+0.007X12- 5.137,
Wherein, Y1It is the center of mass values in the six fort tea sample places of production to be determined, X1It is that the fingerprint characteristic spectrum medium wave is a length of
2924cm-1The wave number at place, X2It is a length of 2920cm of fingerprint characteristic spectrum medium wave-1The wave number at place, X3It is the fingerprint
The a length of 1647cm of characteristic spectrum medium wave-1The wave number at place, X4It is a length of 1452cm of fingerprint characteristic spectrum medium wave-1The wave at place
Value, X5It is a length of 1639cm of fingerprint characteristic spectrum medium wave-1The wave number at place, X6It is the fingerprint characteristic spectrum medium wavelength
For 1367cm-1The wave number at place, X7It is a length of 1325cm of fingerprint characteristic spectrum medium wave-1The wave number at place, X8It is the finger
The a length of 1146cm of line characteristic spectrum medium wave-1The wave number at place, X9It is a length of 1034cm of fingerprint characteristic spectrum medium wave-1The wave at place
Value, X10It is a length of 823cm of fingerprint characteristic spectrum medium wave-1The wave number at place, X11It is the fingerprint characteristic spectrum medium wavelength
For 762cm-1The wave number at place, X12It is a length of 609cm of fingerprint characteristic spectrum medium wave-1The wave number at place；
(5.3) according to the center of mass values Y in the six fort tea sample places of production to be determined1Judge whether six fort tea samples to be determined give birth to
It grows in protection zone.
Preferably, in the step (5.3), as the center of mass values Y in the six fort tea sample places of production of measurement1It is -5.451
When, determine that six fort tea samples to be determined are grown in protection zone, as the center of mass values Y in the six fort tea sample places of production of measurement1
When being 8.176, determine that six fort tea samples to be determined are grown in outside protection zone.
Compared with prior art, the device have the advantages that are as follows:
A kind of of the invention traced to the source using infrared spectroscopy identifies the method in the six fort tea place of production of finished product by six fort tea to be determined
Sample smashes it through screen respectively and obtains tea powder to be determined；Background sample is obtained after grinding filtering drying using the potassium bromide of spectroscopic pure grade
Product；By tea powder to be determined one by one individually and Background Samples mix in proportion after fill sample tabletting obtain tabletting to be detected；By pressure to be detected
Piece obtains infrared spectrogram to be determined after being put in the sample bin scanning of infrared spectrometer one by one；Infrared spectrogram to be determined is carried out pre-
After processing, certain wave bands in the characteristic wave of wave band are chosen as variable, this diagnostic method finds out six forts to be determined according to Weir
Whether the center of mass values in the tea sample place of production, determine and be grown in protection zone.It is of the invention a kind of to be traced to the source knowledge using infrared spectroscopy
The method in the other six fort tea place of production of finished product by will six fort tea samples crush tabletting after carry out infrared spectrum analysis, then obtain infrared light
Characteristic wave bands are analyzed in spectrogram, can effectively identify whether six fort tea tealeaves originate from specified protection zone, are had
The high feature of recognition accuracy.
Detailed description of the invention
Fig. 1 will be numbered as obtained infrared spectroscopy pair after this 30 parts infrared spectroscopy pretreatment figure overlappings to be determined of 1-30
Than figure；
Fig. 2 will be numbered as obtained infrared spectroscopy after this 20 parts infrared spectroscopy pretreatment figure overlappings to be determined of 31-50
Comparison diagram.
Fig. 3 will be numbered as the clustering distribution figure of six fort tea test sample of finished product inside and outside this 50 parts of protection zones 1-50；
Fig. 4 will be numbered as the scatter diagram of six fort tea test sample of finished product inside and outside this 50 parts of protection zones 1-50.
Specific embodiment
With reference to embodiment, technical solution of the present invention is described in further detail, but do not constituted pair
Any restrictions of the invention.
A kind of method for identifying the six fort tea place of production of finished product of being traced to the source using infrared spectroscopy of the invention, is included the following steps:
(1) six fort tea samples to be determined are smashed it through into screen respectively and obtain tea powder to be determined.Wherein, by be determined six
Fort tea sample, which is put into high speed Universal pulverizer, to be crushed, and 100 mesh screens are smashed it through, and filtered tea powder to be determined is put after collecting
Enter in drier and saves for use.
(2) Background Samples are obtained after grinding filtering drying using the potassium bromide (KBr) of spectroscopic pure grade.It wherein, will be by grinding
The potassium bromide of spectroscopic pure grade cross 200 mesh screen below, then carry out drying and processing, drying temperature is 120 DEG C, and drying time is
4 hours or more, again as spare after cooling in drier after drying.
(3) by tea powder to be determined one by one individually and Background Samples mixes in proportion after fill sample tabletting obtain tabletting to be detected, general
When tea powder to be determined and Background Samples mix, sample tabletting is filled after mixing rapidly by weight for 1:100-150.
(4) tabletting to be detected is put in after being scanned in the sample bin of infrared spectrometer one by one and obtains infrared spectrogram to be determined.
The infrared spectrometer is Spectrum GX Fourier infrared spectrograph (Perkin Elemer), spectral region 4000-
400cm-1, scanning signal cumulative 16 times, when scanning, has deducted the interference of water and carbon dioxide, resolution ratio 4cm-1。
(5) after being pre-processed infrared spectrogram to be determined, certain wave bands in the characteristic wave of wave band are chosen as change
Amount finds out the center of mass values in the six fort tea sample places of production to be determined according to Weir this diagnostic method, determines and whether be grown in protection zone
In domain.Wherein, following steps are specifically divided into:
(5.1) infrared spectroscopy pretreatment figure to be determined is obtained after being pre-processed infrared spectrogram to be determined, is such as used
The included analysis software of Spectrum100 type Fourier infrared spectrograph carries out smooth, normalization to primary light spectrogram and pre-processes.
(5.2) obtaining wave band in infrared spectroscopy to be determined pretreatment figure is 3000-2000cm-1、1700-1000cm-1、
850-600cm-1As fingerprint characteristic spectrum, the wave number of some wavelength is obtained respectively in the fingerprint characteristic spectrum, according to
This diagnostic method of Weir finds out the center of mass values in the six fort tea sample places of production to be determined, the calculation formula of center of mass values are as follows:
Y1=-0.185X1-0.333X2+0.32X3+0.45X4+0.016X5+0.477X6+0.016X7+0017X8++
0.016X9-0.008X10+0.05X11+0.007X12- 5.137,
Wherein, Y1It is the center of mass values in the six fort tea sample places of production to be determined, X1It is that the fingerprint characteristic spectrum medium wave is a length of
2924cm-1The wave number at place, X2It is a length of 2920cm of fingerprint characteristic spectrum medium wave-1The wave number at place, X3It is the fingerprint
The a length of 1647cm of characteristic spectrum medium wave-1The wave number at place, X4It is a length of 1452cm of fingerprint characteristic spectrum medium wave-1The wave at place
Value, X5It is a length of 1639cm of fingerprint characteristic spectrum medium wave-1The wave number at place, X6It is the fingerprint characteristic spectrum medium wavelength
For 1367cm-1The wave number at place, X7It is a length of 1325cm of fingerprint characteristic spectrum medium wave-1The wave number at place, X8It is the finger
The a length of 1146cm of line characteristic spectrum medium wave-1The wave number at place, X9It is a length of 1034cm of fingerprint characteristic spectrum medium wave-1The wave at place
Value, X10It is a length of 823cm of fingerprint characteristic spectrum medium wave-1The wave number at place, X11It is the fingerprint characteristic spectrum medium wavelength
For 762cm-1The wave number at place, X12It is a length of 609cm of fingerprint characteristic spectrum medium wave-1The wave number at place.Pass through identification model
Formula calculates the center of mass values of six fort tea samples to be determined, as the six fort tea sample and function center of mass point to be determined away from
From.
(5.3) according to the center of mass values Y in the six fort tea sample places of production to be determined1Judge whether six fort tea samples to be determined give birth to
It grows in protection zone.
After infrared spectroscopy to be determined to be pre-processed to wavelength information substitution this diagnostic method of Weir calculating acquired in figure, obtain
The center of mass values Y in the six fort tea sample places of production to be determined to be determined1, the center of mass values Y in the six fort tea sample places of production more to be determined1
It can judge whether it is grown in protection zone.This diagnostic method of Weir calculates the i.e. corresponding judgement knot of classification of gained center of mass values
Fruit with see Table 1 for details -1, table 1-2.
Table 1-1 identification model discriminant equation verification result
The classification of table 1-2 identification model formula center of mass values
| Center of mass values | Corresponding affiliated area code | Corresponding growth district |
| -5.451 | 1 | In protection zone |
| 8.176 | 2 | Outside protection zone |
As shown in table 1-2, as the center of mass values Y of identification model1When being -5.451, it is corresponding shown in area code be 1, indicate to
Six fort tea samples of measurement are grown in protection zone, as the center of mass values Y of identification model1When being 8.176, corresponding shown region
Code is 2, indicates that six fort tea samples to be determined are grown in outside protection zone.
A kind of of the invention traced to the source using infrared spectroscopy identifies the method in the six fort tea place of production of finished product by by six fort tea samples
Infrared spectrum analysis is carried out after crushing tabletting, then obtains characteristic wave bands in infrared spectrogram and is analyzed, can effectively be identified
Whether six fort tea tealeaves originate from specified protection zone, have the characteristics that recognition accuracy is high.
Test example
Workshop and the storage of 6 enterprises that geographical sign uses are obtained from " six fort tea of geography symbol product " standard
Warehouse is collected the sample using six 30 six fort tea of fort tea finished tea made of raw material in growth self-shield region, is compiled respectively
Number be 1-30.Sample substantially covers the six fort tea finished products from 2014 Nian Houhuobiao enterprises using planting base raw material of putting on record.Together
When, stochastic buying tealeaves form and Guangxi large leaf or Cangwu group are sold in the fairground of six fort tea in the administrative region of Wuzhou
The different cause of body kind, the sample of 10 entitled six fort tea, number is 31-40 respectively.Again from non-using the six of local raw material
The sample of 10 six fort tea is acquired in Bao Cha manufacturing enterprise, number is 41-50 respectively, see Table 1 for details for the sample information of each six fort tea-
2。
The increased made tea sample message list of infrared spectroscopy inside and outside the protection zone table 1-3
50 six fort tea samples to be determined are respectively put into high speed Universal pulverizer and are crushed, the filter of 100 mesh is smashed it through
Sieve to obtain 50 parts of tea powders to be determined.The potassium bromide (potassium bromide) of spectroscopic pure grade is ground, the screen filtering below of 200 mesh is crossed and dries
Dry, drying temperature is 120 DEG C, and drying time is 4 hours or more, after drying again as after cooling in drier Background Samples.
By 50 parts of tea powders to be determined, independent and Background Samples are that 1:100-150 is filled rapidly after mixing by weight one by one
Sample tabletting obtains 50 parts of tablettings to be detected, when tea powder to be determined and Background Samples are mixed, mixes rapidly by weight for 1:100-150
Sample tabletting is filled after closing uniformly.
50 parts of tablettings to be detected are put in after being scanned in the sample bin of infrared spectrometer one by one and obtain 50 infrared lights to be determined
Spectrogram.The infrared spectrometer is Spectrum GX Fourier infrared spectrograph (Perkin Elemer), and spectral region is
4000-400cm-1, scanning signal cumulative 16 times, when scanning, has deducted the interference of water and carbon dioxide, resolution ratio 4cm-1。
50 parts of infrared spectroscopy pretreatment figures to be determined are obtained after 50 parts of infrared spectrograms to be determined are pre-processed respectively, point
It is not obtained infrared spectrum comparison diagram such as Fig. 1 institute after this 30 parts of 1-30 infrared spectroscopy pretreatment figure to be determined is overlapped by number
Show, is obtained infrared spectrum comparison diagram such as Fig. 2 after this 20 parts of 31-50 infrared spectroscopy pretreatment figure to be determined is overlapped by number
It is shown.It can be seen that the Tea Samples inside and outside protection zone are all different from wave number, intensity by Fig. 1 and Fig. 2, there are certain differences
It is different.Obtaining wave band in every part of infrared spectroscopy pretreatment figure to be determined is 3000-2000cm-1、1700-1000cm-1、850-
600cm-1As in fingerprint characteristic spectrum analysis region, tealeaves major absorbance peak in protection zone 2924,2853,1647,
1533、1451、1367、1325、1146、1034、823、762、609cm-1, and the verification sample major absorbance peak outside protection zone
It is 2920,2851,1647,1549,1452,1369,1325,1239,1147,1034,823,762,745cm in wave number-1, from suction
Receiving peak can analyze out, and the chemical composition part of the two is most of or identical, but because of the difference of raw material, respectively
Occur characteristic waves that are different, and analyzing appearance at characteristic absorption, find out difference therein, guarantor can be distinguished to reach
Protect the purpose of six fort tea of finished product within and outside the region.
Wave number in selected characteristic wave band passes through as variable by spss24.0 software to the correlation calculations of data
It can be obtained after Principal Component Analysis (PCA) processing, pc1 and pc2 add up total contribution rate to 75%, extract the first two factor as master
The factor is obtained principal component loading matrix according to what is calculated using spss24.0 software, calculates score of the sample in PC1, PC2,
Drop is drawn into the scatter plot of two-dimensional composition, scatter plot is as shown in Figure 4.Sample 1-30 is distributed in the right, sample 31-50 in scatter plot
It is distributed in the left side, it can be seen that the made tea sample inside and outside protection zone is to illustrate that the two classifications are can to obtain from two classes are divided into
To what is effectively distinguished.If principal component PC1 formula is F1, principal component PC2 formula is F2, if variable 2924cm-1(X1)、2920cm-1
(X2)、1647cm-1(X3)、1452cm-1(X4)、1639cm-1(X5)、1367cm-1(X6)、1325cm-1(X7)、1146cm-1(X8)、
1034cm-1(X9)、823cm-1(X10)、762cm-1(X11)、609cm-1(X12), calculating the score F1 and F2 obtained are as follows:
F1=0.359X1+0.353X2+0.722X3+0.865X4+0.399X5+0.344X6+0.710X7+0.547X8++
0.784X9+0.656X10+0.946X11+0.197X12
F2=0.914X1-0.927X2+0.239X3-0.043X4+0.855X5-0.932X6-0.416X7+0.209X8++
0.018X9-0.046X10-0.141X11+0.828X12
Obtaining wave band in every part of infrared spectroscopy pretreatment figure to be determined is 3000-2000cm-1、1700-1000cm-1、
850-600cm-1As fingerprint characteristic spectrum, the wave number of some wavelength is obtained respectively in the fingerprint characteristic spectrum, carry out
Identification model, a length of 2924cm of print characteristic spectrum medium wave are established in Fisher discriminant analysis-1、2920cm-1、1647cm-1、
1452cm-1、1639cm-1、1367cm-1、1325cm-1、1146cm-1、1034cm-1、823cm-1、762cm-1、609cm-1This is at 12
Wave number, substituted into the formula of identification model, find out the center of mass values Y of the infrared spectroscopy to be determined1, further according to Y1Value sentence
Whether the six fort tea samples to be determined that break are grown in protection zone.The corresponding obtained center of mass values Y of 50 six fort tea samples1With root
According to center of mass values Y1Judge areas case belonging to region and the six fort tea sample reality belonging to it see Table 1 for details -3.
Inside and outside the protection zone in 2017 table 1-4 six fort tea test sample (50) principal component scores figure of finished product and clustering away from
From value
Shown in table 1-3, a kind of method for identifying the six fort tea place of production of finished product of being traced to the source using infrared spectroscopy of the invention is passed through
Infrared spectrum analysis is carried out after six fort tea samples are crushed tabletting, then obtains characteristic wave bands in infrared spectrogram and is analyzed, energy
It is enough effectively to identify whether six fort tea tealeaves originate from specified protection zone, it can be obtained according to data in table, in 50 six fort tea
In the judging result of sample, judicious to have 50, misjudgment has 0, and returning for measurement sample is sentenced rate and reached
100%, recognition accuracy is higher more effective.
The foregoing is merely presently preferred embodiments of the present invention, all made any within the scope of the spirit and principles in the present invention
Modifications, equivalent substitutions and improvements etc., should all be included in the protection scope of the present invention.
Claims (7)
1. a kind of method for identifying the six fort tea place of production of finished product of being traced to the source using infrared spectroscopy, which is characterized in that include the following steps:
(1) six fort tea samples to be determined are smashed it through into screen respectively and obtain tea powder to be determined；
(2) Background Samples are obtained after grinding filtering drying using the potassium bromide of spectroscopic pure grade；
(3) by tea powder to be determined one by one individually and Background Samples mix in proportion after fill sample tabletting obtain tabletting to be detected；
(4) tabletting to be detected is put in after being scanned in the sample bin of infrared spectrometer one by one and obtains infrared spectrogram to be determined；
(5) after being pre-processed infrared spectrogram to be determined, certain wave bands in the characteristic wave of wave band are chosen as variable, root
Whether the center of mass values that the six fort tea sample places of production to be determined are found out according to Weir this diagnostic method, determine and be grown in protection zone.
2. a kind of method for identifying the six fort tea place of production of finished product of being traced to the source using infrared spectroscopy according to claim 1, feature
It is, in the step (1), six fort tea samples to be determined is put into high speed Universal pulverizer and are crushed, are smashed it through
100 mesh screens.
3. a kind of method for identifying the six fort tea place of production of finished product of being traced to the source using infrared spectroscopy according to claim 1, feature
It is, in the step (2), the potassium bromide of the spectroscopic pure grade by grinding is crossed into 200 mesh screen below, then dried
Processing, drying temperature are 120 DEG C, and drying time is 4 hours or more.
4. a kind of method for identifying the six fort tea place of production of finished product of being traced to the source using infrared spectroscopy according to claim 1, feature
It is, in the step (3), the mixed weight ratio of tea powder to be determined and Background Samples is 1:100-150.
5. a kind of method for identifying the six fort tea place of production of finished product of being traced to the source using infrared spectroscopy according to any one of claims 1 to 4,
It is characterized in that, in the step (4), with Spectrum GX Fourier infrared spectrograph (Perkin Elemer), light
Spectral limit 4000-400cm-1, scanning signal is 16 times cumulative, resolution ratio 4cm-1。
6. special according to claim 1 to a kind of method for identifying the six fort tea place of production of finished product of being traced to the source using infrared spectroscopy described in 4
Sign is, in the step (5), is specifically divided into following steps:
(5.1) infrared spectroscopy pretreatment figure to be determined is obtained after being pre-processed infrared spectrogram to be determined；
(5.2) obtaining wave band in infrared spectroscopy to be determined pretreatment figure is 3000-2000cm-1、1700-1000cm-1、850-
600cm-1As fingerprint characteristic spectrum, the wave number of some wavelength is obtained respectively in the fingerprint characteristic spectrum, according to Weir
This diagnostic method finds out the center of mass values in the six fort tea sample places of production to be determined, the calculation formula of center of mass values are as follows:
Y1=-0.185X1-0.333X2+0.32X3+0.45X4+0.016X5+0.477X6+0.016X7+0017X8++0.016X9-
0.008X10+0.05X11+0.007X12- 5.137,
Wherein, Y1It is the center of mass values in the six fort tea sample places of production to be determined, X1It is that the fingerprint characteristic spectrum medium wave is a length of
2924cm-1The wave number at place, X2It is a length of 2920cm of fingerprint characteristic spectrum medium wave-1The wave number at place, X3It is the fingerprint
The a length of 1647cm of characteristic spectrum medium wave-1The wave number at place, X4It is a length of 1452cm of fingerprint characteristic spectrum medium wave-1The wave at place
Value, X5It is a length of 1639cm of fingerprint characteristic spectrum medium wave-1The wave number at place, X6It is the fingerprint characteristic spectrum medium wavelength
For 1367cm-1The wave number at place, X7It is a length of 1325cm of fingerprint characteristic spectrum medium wave-1The wave number at place, X8It is the finger
The a length of 1146cm of line characteristic spectrum medium wave-1The wave number at place, X9It is a length of 1034cm of fingerprint characteristic spectrum medium wave-1The wave at place
Value, X10It is a length of 823cm of fingerprint characteristic spectrum medium wave-1The wave number at place, X11It is the fingerprint characteristic spectrum medium wavelength
For 762cm-1The wave number at place, X12It is a length of 609cm of fingerprint characteristic spectrum medium wave-1The wave number at place；
(5.3) according to the center of mass values Y in the six fort tea sample places of production to be determined1Judge whether six fort tea samples to be determined are grown in
In protection zone.
7. a kind of method for identifying the six fort tea place of production of finished product of being traced to the source using infrared spectroscopy according to claim 6, feature
It is, in the step (5.3), as the center of mass values Y in the six fort tea sample places of production of measurement1When being -5.451, determine to be measured
Six fixed fort tea samples are grown in protection zone, as the center of mass values Y in the six fort tea sample places of production of measurement1When being 8.176, really
Fixed six fort tea samples to be determined are grown in outside protection zone.
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