CN109355267A - A kind of liquid enzyme formulation and its preparation method and application - Google Patents
A kind of liquid enzyme formulation and its preparation method and application Download PDFInfo
- Publication number
- CN109355267A CN109355267A CN201810972705.8A CN201810972705A CN109355267A CN 109355267 A CN109355267 A CN 109355267A CN 201810972705 A CN201810972705 A CN 201810972705A CN 109355267 A CN109355267 A CN 109355267A
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- Prior art keywords
- liquid
- protease
- glutamine transaminage
- aqueous solution
- preparation
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- 102000004190 Enzymes Human genes 0.000 title claims abstract description 284
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 284
- 239000007788 liquid Substances 0.000 title claims abstract description 213
- 238000002360 preparation method Methods 0.000 title claims abstract description 130
- 238000009472 formulation Methods 0.000 title claims abstract description 125
- 239000000203 mixture Substances 0.000 title claims abstract description 125
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 claims abstract description 206
- 108091005804 Peptidases Proteins 0.000 claims abstract description 199
- 230000000694 effects Effects 0.000 claims abstract description 184
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 165
- 239000004365 Protease Substances 0.000 claims abstract description 156
- 235000019419 proteases Nutrition 0.000 claims abstract description 152
- 239000007864 aqueous solution Substances 0.000 claims abstract description 121
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 63
- 102000035195 Peptidases Human genes 0.000 claims abstract description 34
- 235000019833 protease Nutrition 0.000 claims abstract description 34
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims abstract description 25
- 229910052708 sodium Inorganic materials 0.000 claims abstract description 25
- 239000011734 sodium Substances 0.000 claims abstract description 25
- CIWBSHSKHKDKBQ-DUZGATOHSA-N D-araboascorbic acid Natural products OC[C@@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-DUZGATOHSA-N 0.000 claims abstract description 22
- 235000010350 erythorbic acid Nutrition 0.000 claims abstract description 22
- DINKXUCRJBUQAZ-UHFFFAOYSA-N tert-butyl 5-bromopyridine-3-carboxylate Chemical compound CC(C)(C)OC(=O)C1=CN=CC(Br)=C1 DINKXUCRJBUQAZ-UHFFFAOYSA-N 0.000 claims abstract description 22
- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 claims abstract description 21
- 229920001661 Chitosan Polymers 0.000 claims abstract description 21
- 229920002472 Starch Polymers 0.000 claims abstract description 21
- 239000005018 casein Substances 0.000 claims abstract description 21
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 claims abstract description 21
- 235000021240 caseins Nutrition 0.000 claims abstract description 21
- 239000001866 hydroxypropyl methyl cellulose Substances 0.000 claims abstract description 21
- 235000010979 hydroxypropyl methyl cellulose Nutrition 0.000 claims abstract description 21
- 229920003088 hydroxypropyl methyl cellulose Polymers 0.000 claims abstract description 21
- 239000000787 lecithin Substances 0.000 claims abstract description 21
- 235000010445 lecithin Nutrition 0.000 claims abstract description 21
- 229940067606 lecithin Drugs 0.000 claims abstract description 21
- 235000019698 starch Nutrition 0.000 claims abstract description 21
- 239000008107 starch Substances 0.000 claims abstract description 21
- 229920002774 Maltodextrin Polymers 0.000 claims abstract description 20
- 239000005913 Maltodextrin Substances 0.000 claims abstract description 20
- 229940035034 maltodextrin Drugs 0.000 claims abstract description 20
- JXKPEJDQGNYQSM-UHFFFAOYSA-M sodium propionate Chemical compound [Na+].CCC([O-])=O JXKPEJDQGNYQSM-UHFFFAOYSA-M 0.000 claims abstract description 20
- 108010073771 Soybean Proteins Proteins 0.000 claims abstract description 19
- 235000010334 sodium propionate Nutrition 0.000 claims abstract description 19
- 239000004324 sodium propionate Substances 0.000 claims abstract description 19
- 229960003212 sodium propionate Drugs 0.000 claims abstract description 19
- 235000019710 soybean protein Nutrition 0.000 claims abstract description 19
- 239000001814 pectin Substances 0.000 claims abstract description 18
- 235000010987 pectin Nutrition 0.000 claims abstract description 18
- 229920001277 pectin Polymers 0.000 claims abstract description 18
- 235000013305 food Nutrition 0.000 claims abstract description 4
- UFVKGYZPFZQRLF-UHFFFAOYSA-N hydroxypropyl methyl cellulose Chemical compound OC1C(O)C(OC)OC(CO)C1OC1C(O)C(O)C(OC2C(C(O)C(OC3C(C(O)C(O)C(CO)O3)O)C(CO)O2)O)C(CO)O1 UFVKGYZPFZQRLF-UHFFFAOYSA-N 0.000 claims abstract 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 46
- 239000000243 solution Substances 0.000 claims description 27
- 238000003756 stirring Methods 0.000 claims description 27
- 238000000034 method Methods 0.000 claims description 23
- 239000004615 ingredient Substances 0.000 claims description 10
- 108010010779 glutamine-pyruvate aminotransferase Proteins 0.000 claims description 8
- 108090000340 Transaminases Proteins 0.000 claims description 7
- 102000003929 Transaminases Human genes 0.000 claims description 7
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims description 4
- 239000003795 chemical substances by application Substances 0.000 claims description 4
- 229910021529 ammonia Inorganic materials 0.000 claims description 3
- 235000013399 edible fruits Nutrition 0.000 claims description 3
- 239000003292 glue Substances 0.000 claims description 3
- 229940079593 drug Drugs 0.000 claims description 2
- 239000003814 drug Substances 0.000 claims description 2
- 235000010469 Glycine max Nutrition 0.000 claims 1
- 244000068988 Glycine max Species 0.000 claims 1
- 101710094902 Legumin Proteins 0.000 claims 1
- 108010009736 Protein Hydrolysates Proteins 0.000 claims 1
- NEKNNCABDXGBEN-UHFFFAOYSA-L disodium;4-(4-chloro-2-methylphenoxy)butanoate;4-(2,4-dichlorophenoxy)butanoate Chemical compound [Na+].[Na+].CC1=CC(Cl)=CC=C1OCCCC([O-])=O.[O-]C(=O)CCCOC1=CC=C(Cl)C=C1Cl NEKNNCABDXGBEN-UHFFFAOYSA-L 0.000 claims 1
- 239000000047 product Substances 0.000 claims 1
- 239000003531 protein hydrolysate Substances 0.000 claims 1
- 238000003860 storage Methods 0.000 abstract description 64
- 229940088598 enzyme Drugs 0.000 description 257
- 230000000052 comparative effect Effects 0.000 description 21
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 19
- 238000012360 testing method Methods 0.000 description 17
- HSHXDCVZWHOWCS-UHFFFAOYSA-N N'-hexadecylthiophene-2-carbohydrazide Chemical compound CCCCCCCCCCCCCCCCNNC(=O)c1cccs1 HSHXDCVZWHOWCS-UHFFFAOYSA-N 0.000 description 16
- 235000015278 beef Nutrition 0.000 description 15
- 235000013372 meat Nutrition 0.000 description 9
- 235000013622 meat product Nutrition 0.000 description 7
- 238000010008 shearing Methods 0.000 description 7
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 238000001514 detection method Methods 0.000 description 6
- 235000018102 proteins Nutrition 0.000 description 5
- 102000004169 proteins and genes Human genes 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 4
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 4
- 230000003247 decreasing effect Effects 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 239000003002 pH adjusting agent Substances 0.000 description 4
- 150000001412 amines Chemical class 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- YIWGJFPJRAEKMK-UHFFFAOYSA-N 1-(2H-benzotriazol-5-yl)-3-methyl-8-[2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carbonyl]-1,3,8-triazaspiro[4.5]decane-2,4-dione Chemical compound CN1C(=O)N(c2ccc3n[nH]nc3c2)C2(CCN(CC2)C(=O)c2cnc(NCc3cccc(OC(F)(F)F)c3)nc2)C1=O YIWGJFPJRAEKMK-UHFFFAOYSA-N 0.000 description 2
- BVKZGUZCCUSVTD-UHFFFAOYSA-M Bicarbonate Chemical compound OC([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-M 0.000 description 2
- 108010004032 Bromelains Proteins 0.000 description 2
- DEOKFPFLXFNAON-UHFFFAOYSA-N N-α-Benzoyl-DL-arginine 4-nitroanilide hydrochloride Chemical compound Cl.C=1C=C([N+]([O-])=O)C=CC=1NC(=O)C(CCCN=C(N)N)NC(=O)C1=CC=CC=C1 DEOKFPFLXFNAON-UHFFFAOYSA-N 0.000 description 2
- 108090000526 Papain Proteins 0.000 description 2
- 102000057297 Pepsin A Human genes 0.000 description 2
- 108090000284 Pepsin A Proteins 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 235000019835 bromelain Nutrition 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000000151 deposition Methods 0.000 description 2
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 2
- 235000019834 papain Nutrition 0.000 description 2
- 229940055729 papain Drugs 0.000 description 2
- 229940111202 pepsin Drugs 0.000 description 2
- 239000002504 physiological saline solution Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M sodium bicarbonate Substances [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 2
- 235000017557 sodium bicarbonate Nutrition 0.000 description 2
- 229910000029 sodium carbonate Inorganic materials 0.000 description 2
- -1 trypsase Proteins 0.000 description 2
- 241000700199 Cavia porcellus Species 0.000 description 1
- 229920001353 Dextrin Polymers 0.000 description 1
- 239000004375 Dextrin Substances 0.000 description 1
- 108020004206 Gamma-glutamyltransferase Proteins 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- 206010054949 Metaplasia Diseases 0.000 description 1
- GXCLVBGFBYZDAG-UHFFFAOYSA-N N-[2-(1H-indol-3-yl)ethyl]-N-methylprop-2-en-1-amine Chemical compound CN(CCC1=CNC2=C1C=CC=C2)CC=C GXCLVBGFBYZDAG-UHFFFAOYSA-N 0.000 description 1
- 108060008539 Transglutaminase Proteins 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 230000002745 absorbent Effects 0.000 description 1
- 125000002252 acyl group Chemical group 0.000 description 1
- 150000001408 amides Chemical class 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 238000005336 cracking Methods 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 235000019425 dextrin Nutrition 0.000 description 1
- 238000001952 enzyme assay Methods 0.000 description 1
- 108010032995 epsilon-(gamma-glutamyl)-lysine Proteins 0.000 description 1
- JPKNLFVGUZRHOB-YUMQZZPRSA-N epsilon-(gamma-glutamyl)lysine Chemical compound OC(=O)[C@@H](N)CCCCNC(=O)CC[C@H](N)C(O)=O JPKNLFVGUZRHOB-YUMQZZPRSA-N 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000005187 foaming Methods 0.000 description 1
- 235000013373 food additive Nutrition 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- 102000006640 gamma-Glutamyltransferase Human genes 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 230000015689 metaplastic ossification Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 230000003014 reinforcing effect Effects 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 102000003601 transglutaminase Human genes 0.000 description 1
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1025—Acyltransferases (2.3)
- C12N9/104—Aminoacyltransferases (2.3.2)
- C12N9/1044—Protein-glutamine gamma-glutamyltransferase (2.3.2.13), i.e. transglutaminase or factor XIII
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/63—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6427—Chymotrypsins (3.4.21.1; 3.4.21.2); Trypsin (3.4.21.4)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6478—Aspartic endopeptidases (3.4.23)
- C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y203/00—Acyltransferases (2.3)
- C12Y203/02—Aminoacyltransferases (2.3.2)
- C12Y203/02013—Protein-glutamine gamma-glutamyltransferase (2.3.2.13), i.e. transglutaminase or factor XIII
-
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21004—Trypsin (3.4.21.4)
-
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/22—Cysteine endopeptidases (3.4.22)
- C12Y304/22002—Papain (3.4.22.2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/22—Cysteine endopeptidases (3.4.22)
- C12Y304/22004—Bromelain (3.4.22.4)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/23—Aspartic endopeptidases (3.4.23)
- C12Y304/23001—Pepsin A (3.4.23.1)
Abstract
The invention discloses a kind of liquid enzyme formulations and its preparation method and application, belong to food processing technology field.Liquid enzyme formulation of the invention (comprising glutamine transaminage aqueous solution that glutamine transaminage enzyme activity is 10~1000U/mL and accounts for the starch of glutamine transaminage aqueous solution quality 5~10% by glutamine transaminage liquid preparation, 3~5% pectin, 0.1~3% hydroxypropyl methylcellulose, 1~5% D-araboascorbic acid sodium, 0.5~2% soybean protein hydrolyate, 0.5~1% lecithin) and liquid of protease body preparation (comprising aqueous solution of protease that proteinase activity is 10~1000U/mL and account for the glycerol of aqueous solution of protease quality 15~30%, 3~5% chitosan, 0.1~1% casein, 0.5~1% sodium propionate, 3~8% maltodextrin) it constitutes；Liquid enzyme formulation of the invention is placed 8 months under room temperature, glutamine transaminage and proteinase activity storage rate remain at 75% or so.
Description
Technical field
The present invention relates to a kind of liquid enzyme formulations and its preparation method and application, belong to food processing technology field.
Background technique
Glutamine transaminage (EC.2.3.2.13, full name in English Amine γ-glutamyl-transferase, English
Abbreviation Transglutaminase, abridge entitled TG) it is a kind of protein-crosslinking enzyme, it can be with ε-ammonia of lysine in protein peptide chain
Base is acyl acceptor, catalytic proteins intramolecular and the intermolecular special-shaped peptide bond for forming ε-(γ-glutamyl) lysine, is improved
A variety of properties such as emulsibility, rheological characteristic, dissolubility, the foaming characteristic of protein assign the distinctive texture characteristic of protein and bonding
Property, the color, smell and taste of product are improved, there is very big market value.
Glutamine transaminage be earliest it is isolated from Guinea Pig Liver by Clarke et al., 1989 years by Japan
The Ando et al. of Amano company has invented Production by Microorganism Fermentation glutamine transaminage, and 1997 public by Japanese aginomoto
Department realizes the production of commercial size metaplasia, and glutamine transaminage is formally classified as food additives by China in 2001.
Currently, the form that glutamine transaminage is often made into powder formulation is used and is circulated, powder formulation
Also have been carried out large-scale production, and be widely applied in food-processing industry, but its production, transport, storage, in
There is also many problems.
For example, when be used to make meat products, since glutamine transaminage is difficult to and more coarse, meat is harder
Low-grade meat products react completely so that it can only play good result in the relatively good meat products of medium-to-high grade meat, and one
As the relatively good meat products of medium-to-high grade meat do not need excessive additional processing, the low-grade meat products coarse, meat is harder
The main body for needing processing is only in meat products production, this defect of glutamine transaminage undoubtedly limits it in meat products processing
In application.
But, scholar solves the problems, such as this, section Maohua et al. is by with protease and glutamine transaminage
Beef is first post-processed, the moisture of beef is significantly improved, is waterpower, water activity, shearing force and texture intensity (protease and TG
Enzyme is to the modified synergic technology of beef protein and application study, " Guizhou University ", 2008).Therefore, one kind is developed to contain simultaneously
The enzyme preparation of glutamine transaminage and protease has huge market potential.
But we have found in early-stage study, glutamine transaminage and protease effective object each other, by glutamy
When amine transaminase and protease put together preparation enzyme preparation, they can be consumed mutually, so that enzyme preparation glutamine turns
Adnosine deaminase and the enzyme activity of protease are remarkably decreased, until enzyme activity disappears.Therefore, it develops one kind and contains glutamine simultaneously
Transaminase and the enzyme preparation of protease still need to further study.
Summary of the invention
To solve the above problems, the present invention provides a kind of liquid enzyme formulations and its preparation method and application.This liquid enzymes
Preparation (is turned by glutamine transaminage liquid preparation comprising the glutamine that glutamine transaminage enzyme activity is 10~1000U/mL
Adnosine deaminase aqueous solution and account for glutamine transaminage aqueous solution quality 5~10% starch, 3~5% pectin, 0.1~3%
Hydroxypropyl methylcellulose, 1~5% D-araboascorbic acid sodium, 0.5~2% soybean protein hydrolyate, 0.5~1% lecithin)
And (comprising aqueous solution of protease that proteinase activity is 10~1000U/mL and to account for protease water-soluble for liquid of protease body preparation
The glycerol of liquid quality 15~30%, 3~5% chitosan, 0.1~1% casein, 0.1~1% sodium propionate, 3~8%
Maltodextrin) constitute；This liquid enzyme formulation is placed 8 months under room temperature, glutamine transaminage and proteinase activity are protected
The rate of depositing remains at 75% or so.
Technical scheme is as follows:
The present invention provides a kind of liquid enzyme formulation, the ingredient of the liquid enzyme formulation includes glutamine transaminage liquid
Preparation and liquid of protease body preparation；
The ingredient of the glutamine transaminage liquid preparation includes that glutamine transaminage enzyme activity is 10~1000U/mL
Glutamine transaminage aqueous solution and account for 5~10% starch of glutamine transaminage aqueous solution quality, 3~5% fruit
Glue, 0.1~3% hydroxypropyl methylcellulose, 1~5% D-araboascorbic acid sodium, 0.5~2% soybean protein hydrolyate, 0.5~
1% lecithin；
The ingredient of the liquid of protease body preparation include proteinase activity be 10~1000U/mL aqueous solution of protease with
And account for the glycerol of aqueous solution of protease quality 15~30%, 3~5% chitosan, 0.1~1% casein, 0.1~1%
Sodium propionate, 3~8% maltodextrin.
In one embodiment of the invention, the ingredient of the glutamine transaminage liquid preparation includes glutamine
Transaminase enzyme activity is the glutamine transaminage aqueous solution of 500U/mL and accounts for glutamine transaminage aqueous solution quality 8%
Starch, 3.5% pectin, 1% hydroxypropyl methylcellulose, 2% D-araboascorbic acid sodium, 1.5% soybean protein hydrolyate,
0.6% lecithin；
The ingredient of the liquid of protease body preparation includes the aqueous solution of protease and account for egg that proteinase activity is 400U/mL
Glycerol, 4.5% chitosan, 0.8% casein, 0.5% sodium propionate, 5% malt of white enzyme aqueous solution quality 20%
Dextrin.
In one embodiment of the invention, the liquid enzyme formulation glutamine transaminase liquid preparation and albumen
The volume ratio of enzyme solution body preparation is 5~30:1.
In one embodiment of the invention, the liquid enzyme formulation glutamine transaminase liquid preparation and albumen
The volume ratio of enzyme solution body preparation is 10:1.
In one embodiment of the invention, the pH of the liquid enzyme formulation is 5~7.
In one embodiment of the invention, the pH of the liquid enzyme formulation is 6.
In one embodiment of the invention, the pH of the pH liquid enzyme formulation can be by additional in liquid enzyme formulation
PH adjusting agent is added to be adjusted, the pH adjusting agent includes hydrochloric acid, sulfuric acid, acetic acid, sodium hydroxide, sodium carbonate or bicarbonate
One of sodium is a variety of.
In one embodiment of the invention, the protease include pepsin, trypsase, papain or
One of bromelain is a variety of.
The present invention provides a kind of above-mentioned preparation method of liquid enzyme formulation, the method is that glutamine is added in water
Transaminase is mixed, and the glutamine transaminage aqueous solution that glutamine transaminage enzyme activity is 10~1000U/mL is obtained；?
It is added in glutamine transaminage aqueous solution and accounts for 5~10% starch of glutamine transaminage aqueous solution quality, 3~5% fruit
Glue, 0.1~3% hydroxypropyl methylcellulose, 1~5% D-araboascorbic acid sodium, 0.5~2% soybean protein hydrolyate, 0.5~
1% lecithin stirs 5~10min under 80~150r/min revolving speed, obtains glutamine transaminage liquid preparation；
Protease is added in water to be mixed, the aqueous solution of protease that proteinase activity is 10~1000U/mL is obtained；
In aqueous solution of protease be added account for aqueous solution of protease quality 15~30% glycerol, 3~5% chitosan, 0.1~1%
Casein, 0.1~1% sodium propionate, 3~8% maltodextrin, under 80~150r/min revolving speed stir 5~10min,
Obtain liquid of protease body preparation；
Glutamine transaminage liquid preparation is mixed with liquid of protease body preparation according to certain volume ratio, is obtained
Liquid enzyme formulation.
In one embodiment of the invention, the method is that glutamine transaminage is added in water to be mixed,
Obtain the glutamine transaminage aqueous solution that glutamine transaminage enzyme activity is 500U/mL；In glutamine transaminage aqueous solution
Middle addition account for glutamine transaminage aqueous solution quality 8% starch, 3.5% pectin, 1% hydroxypropyl methylcellulose, 2%
D-araboascorbic acid sodium, 1.5% soybean protein hydrolyate, 0.6% lecithin, stir 8min under 100r/min revolving speed, obtain
To glutamine transaminage liquid preparation；
Protease is added in water to be mixed, the aqueous solution of protease that proteinase activity is 400U/mL is obtained；In albumen
The glycerol, 4.5% chitosan, 0.8% casein, 0.5% for accounting for aqueous solution of protease quality 20% are added in enzyme aqueous solution
Sodium propionate, 5% maltodextrin, stir 10min under 120r/min revolving speed, obtain liquid of protease body preparation.
In one embodiment of the invention, the liquid enzyme formulation glutamine transaminase liquid preparation and albumen
The volume ratio of enzyme solution body preparation is 5~30:1.
In one embodiment of the invention, the liquid enzyme formulation glutamine transaminase liquid preparation and albumen
The volume ratio of enzyme solution body preparation is 10:1.
In one embodiment of the invention, the pH of the liquid enzyme formulation is 5~7.
In one embodiment of the invention, the pH of the liquid enzyme formulation is 6.
In one embodiment of the invention, the pH of the pH liquid enzyme formulation can be by additional in liquid enzyme formulation
PH adjusting agent is added to be adjusted, the pH adjusting agent includes hydrochloric acid, sulfuric acid, acetic acid, sodium hydroxide, sodium carbonate or bicarbonate
One of sodium is a variety of.
In one embodiment of the invention, the protease include pepsin, trypsase, papain or
One of bromelain is a variety of.
The present invention provides apply a kind of above-mentioned liquid enzyme formulation that the preparation method of liquid enzyme formulation is prepared.
The present invention provides the preparation methods or above-mentioned system of a kind of above-mentioned liquid enzyme formulation or a kind of above-mentioned liquid enzyme formulation
Application of the standby obtained liquid enzyme formulation in terms of preparing food, drug, health care product.
The utility model has the advantages that
(1) present invention successfully solves in the liquid enzyme formulation simultaneously containing glutamine transaminage and protease, paddy ammonia
The problem of amide transaminase and protease mutually consume, liquid enzyme formulation of the invention is placed 6 months under room temperature, glutamy
Amine transaminase and proteinase activity storage rate remain at 85% or so, and liquid enzyme formulation of the invention is placed 8 under room temperature
A month, glutamine transaminage and proteinase activity storage rate remained at 80% or so；
(2) present invention successfully solves the defect for being difficult to ensure and depositing under the room temperature that glutamine transaminage itself has, this is sent out
Bright liquid enzyme formulation is placed 6 months under room temperature, and the enzyme activity storage rate of glutamine transaminage remains at 80% or so, will
Liquid enzyme formulation of the invention is placed 8 months under room temperature, and the enzyme activity storage rate of glutamine transaminage remains at 75% left side
It is right；
(3) liquid enzyme formulation using effect of the invention is good, handles beef 2h with liquid enzyme formulation of the invention, can be bright
Aobvious water activity, shearing force and the texture intensity for improving beef, can by the water activity of beef from 0.81Aw/% improve to
0.89Aw/%, shearing force are reduced to 15N from 38N, and texture intensity is from 18g/mm2It improves to 42g/mm2。
Specific embodiment
The present invention will be further elaborated combined with specific embodiments below.
Detection method of the present invention is as follows:
Glutamine transaminage enzyme activity determination method:
Enzyme activity determination condition: under the conditions of 37 DEG C, 40 μ L enzyme solutions, 100 μ L 30mM α-N-CBZ-GLN-GLY reaction
40 μ L terminator (3M HCl, 12% trichloroacetic acid, 5%FeCl are added in 10min3) reaction is terminated, extinction is measured at 525nm
Value draws standard curve by the mono- light amino acid of Pidolidone-γ-, calculates enzyme activity according to standard curve.
The enzyme activity of 1 unit is defined as: under conditions of 37 DEG C, be catalyzed α-N-CBZ-GLN-GLY per minute and synthesize 1 μm of ol
The mono- light amino acid of Pidolidone-γ-used in enzyme amount (U/mL).
Trypsase enzyme activity determination method:
Enzyme activity determination condition: at 37 DEG C, 100 μ L enzyme solutions (are dissolved in 900 μ L 10mM BAPNA solution
50mMpH8.0Tris-HCl buffer) in the reaction tank of optical path 0.5cm 10min is reacted, light absorption value is measured at 410nm, is led to
It crosses paranitroanilinum and draws standard curve, enzyme activity is calculated according to standard curve.
The enzyme activity of 1 unit is defined as: at 37 DEG C, be catalyzed BAPNA per minute and synthesize used in 1 μm of ol paranitroanilinum
Enzyme amount (U/mL).
(Enzyme activity assay of remaining protease can refer to People's Republic of China's professional standard SB/T10317-1999 protease
Vigour-testing method 2007)
It is hydraulic detection method:
Sample meat piece center sample (about 10mm is thick) is taken with circular hole sampler, claims sample with electronic induction balance, is placed on sense of rotation
On power device, the enough absorbent filters of sample underlay, reinforcing is kept removing pressure after 5min and claims sample again to 35kg.
Water activity detection method:
By sample HD-3A water activity detector test.
Shear force detection method:
Method of the sample perpendicular to muscle fibre direction, the sampling of standard circular hole sampler is sampled, thickness about 20mm uses C-
The detection of LMZ tender degree device.
Texture strength detecting method:
Sampling: beef is cut into the meat cubelets of 40mm*40mm*45mm with knife by identical lines；
Pressurization: meat cubelets being laid flat, then put a plate on meat cubelets, pressure is then gradually increased on plate, until meat cubelets
Until cannot restoring and cracking, obtained load values are its elastic critical point, texture intensity=load values/section
Area.
Embodiment 1: the preparation of liquid enzyme formulation
Specific step is as follows:
(1) glutamine transaminage is added in water to be mixed, obtaining glutamine transaminage enzyme activity is 500U/mL's
Glutamine transaminage aqueous solution；
(2) in glutamine transaminage aqueous solution be added account for glutamine transaminage aqueous solution quality 8% starch,
3.5% pectin, 1% hydroxypropyl methylcellulose, 2% D-araboascorbic acid sodium, 1.5% soybean protein hydrolyate, 0.6%
Lecithin stirs 8min under 100r/min revolving speed, obtains glutamine transaminage liquid preparation；
(3) protease is added in water to be mixed, obtains the aqueous solution of protease that proteinase activity is 400U/mL；
(4) in aqueous solution of protease be added account for aqueous solution of protease quality 20% glycerol, 4.5% chitosan,
0.8% casein, 0.5% sodium propionate, 5% maltodextrin stir 10min under 120r/min revolving speed, obtain albumen
Enzyme solution body preparation；
(5) glutamine transaminage liquid preparation is mixed with liquid of protease body preparation according to the volume ratio of 10:1,
And the HCl solution that concentration is 1M is added and adjusts pH to 6, obtain liquid enzyme formulation.
Obtained liquid enzyme formulation is placed in 25 DEG C, and is spaced one month and detects its glutamine transaminage and protease
Enzyme activity storage rate (the enzyme activity * 100% measured when the enzyme activity/0h measured when enzyme activity storage rate=xth h), testing result such as table
1。
Such as table 1, in liquid enzyme formulation treated by the present method, the enzyme activity of glutamine transaminage and protease saves good
It is good, it is saved 8 months under room temperature, glutamine transaminage enzyme activity storage rate is up to 86%, and proteinase activity storage rate is up to 89%.
The enzyme activity storage rate of the enzyme in liquid enzyme formulation under the conditions of 25 DEG C of table 1
Embodiment 2: the preparation of liquid enzyme formulation
Specific step is as follows:
(1) glutamine transaminage is added in water to be mixed, obtaining glutamine transaminage enzyme activity is 500U/mL's
Glutamine transaminage aqueous solution；
(2) in glutamine transaminage aqueous solution be added account for glutamine transaminage aqueous solution quality 5% starch,
3% pectin, 0.1% hydroxypropyl methylcellulose, 1% D-araboascorbic acid sodium, 0.5% soybean protein hydrolyate, 0.5%
Lecithin stirs 8min under 100r/min revolving speed, obtains glutamine transaminage liquid preparation；
(3) protease is added in water to be mixed, obtains the aqueous solution of protease that proteinase activity is 400U/mL；
(4) in aqueous solution of protease be added account for aqueous solution of protease quality 15% glycerol, 3% chitosan, 0.1%
Casein, 0.1% sodium propionate, 3% maltodextrin, stir 10min under 120r/min revolving speed, obtain protease liquid
Preparation；
(5) glutamine transaminage liquid preparation is mixed with liquid of protease body preparation according to the volume ratio of 10:1,
And the HCl solution that concentration is 1M is added and adjusts pH to 6, obtain liquid enzyme formulation.
Obtained liquid enzyme formulation is placed in 25 DEG C, and is spaced one month and detects its glutamine transaminage and protease
Enzyme activity storage rate (the enzyme activity * 100% measured when the enzyme activity/0h measured when enzyme activity storage rate=xth h), testing result such as table
2。
Such as table 2, in liquid enzyme formulation treated by the present method, the enzyme activity of glutamine transaminage and protease saves good
It is good, it is saved 8 months under room temperature, glutamine transaminage enzyme activity storage rate is up to 77%, and proteinase activity storage rate is up to 80%.
The enzyme activity storage rate of the enzyme in liquid enzyme formulation under the conditions of 25 DEG C of table 2
Embodiment 3: the preparation of liquid enzyme formulation
Specific step is as follows:
(1) glutamine transaminage is added in water to be mixed, obtaining glutamine transaminage enzyme activity is 500U/mL's
Glutamine transaminage aqueous solution；
(2) in glutamine transaminage aqueous solution be added account for glutamine transaminage aqueous solution quality 10% starch,
5% pectin, 3% hydroxypropyl methylcellulose, 5% D-araboascorbic acid sodium, 2% soybean protein hydrolyate, 1% lecithin
Rouge stirs 8min under 100r/min revolving speed, obtains glutamine transaminage liquid preparation；
(3) protease is added in water to be mixed, obtains the aqueous solution of protease that proteinase activity is 400U/mL；
(4) be added in aqueous solution of protease the glycerol, 5% that account for aqueous solution of protease quality 30% chitosan, 1%
Casein, 1% sodium propionate, 8% maltodextrin stir 10min under 120r/min revolving speed, obtain liquid of protease system
Agent；
(5) glutamine transaminage liquid preparation is mixed with liquid of protease body preparation according to the volume ratio of 10:1,
And the HCl solution that concentration is 1M is added and adjusts pH to 5.5, obtain liquid enzyme formulation.
Obtained liquid enzyme formulation is placed in 25 DEG C, and is spaced one month and detects its glutamine transaminage and protease
Enzyme activity storage rate (the enzyme activity * 100% measured when the enzyme activity/0h measured when enzyme activity storage rate=xth h), testing result such as table
3。
Such as table 3, in liquid enzyme formulation treated by the present method, the enzyme activity of glutamine transaminage and protease saves good
It is good, saved 8 months under room temperature, glutamine transaminage enzyme activity storage rate up to 87%, proteinase activity storage rate up to 88%,
But this scheme cost is excessively high, considers cost, unsuitable industrial implementation.
The enzyme activity storage rate of the enzyme in liquid enzyme formulation under the conditions of 25 DEG C of table 3
Embodiment 4: the preparation of liquid enzyme formulation
Specific step is as follows:
(1) glutamine transaminage is added in water to be mixed, obtaining glutamine transaminage enzyme activity is 500U/mL's
Glutamine transaminage aqueous solution；
(2) in glutamine transaminage aqueous solution be added account for glutamine transaminage aqueous solution quality 6% starch,
4% pectin, 0.8% hydroxypropyl methylcellulose, 1.5% D-araboascorbic acid sodium, 0.7% soybean protein hydrolyate, 0.6%
Lecithin, stir 10min under 100r/min revolving speed, obtain glutamine transaminage liquid preparation；
(3) protease is added in water to be mixed, obtains the aqueous solution of protease that proteinase activity is 400U/mL；
(4) in aqueous solution of protease be added account for aqueous solution of protease quality 18% glycerol, 4% chitosan, 0.5%
Casein, 0.3% sodium propionate, 4% maltodextrin, stir 10min under 120r/min revolving speed, obtain protease liquid
Preparation；
(5) glutamine transaminage liquid preparation is mixed with liquid of protease body preparation according to the volume ratio of 15:1,
And the HCl solution that concentration is 1M is added and adjusts pH to 7, obtain liquid enzyme formulation.
Obtained liquid enzyme formulation is placed in 25 DEG C, and is spaced one month and detects its glutamine transaminage and protease
Enzyme activity storage rate (the enzyme activity * 100% measured when the enzyme activity/0h measured when enzyme activity storage rate=xth h), testing result such as table
4。
Such as table 4, in liquid enzyme formulation treated by the present method, the enzyme activity of glutamine transaminage and protease saves good
It is good, it is saved 8 months under room temperature, glutamine transaminage enzyme activity storage rate is up to 81%, and proteinase activity storage rate is up to 84%.
The enzyme activity storage rate of the enzyme in liquid enzyme formulation under the conditions of 25 DEG C of table 4
Embodiment 5: the preparation of liquid enzyme formulation
Specific step is as follows:
(1) glutamine transaminage is added in water to be mixed, obtaining glutamine transaminage enzyme activity is 500U/mL's
Glutamine transaminage aqueous solution；
(2) in glutamine transaminage aqueous solution be added account for glutamine transaminage aqueous solution quality 10% starch,
3% pectin, 2% hydroxypropyl methylcellulose, 3% D-araboascorbic acid sodium, 1% soybean protein hydrolyate, 0.8% lecithin
Rouge stirs 10min under 100r/min revolving speed, obtains glutamine transaminage liquid preparation；
(3) protease is added in water to be mixed, obtains the aqueous solution of protease that proteinase activity is 400U/mL；
(4) in aqueous solution of protease be added account for aqueous solution of protease quality 25% glycerol, 2.5% chitosan,
0.6% casein, 0.8% sodium propionate, 6% maltodextrin stir 10min under 120r/min revolving speed, obtain albumen
Enzyme solution body preparation；
(5) glutamine transaminage liquid preparation is mixed with liquid of protease body preparation according to the volume ratio of 5:1, and
The HCl solution that concentration is 1M is added and adjusts pH to 5.5, obtains liquid enzyme formulation.
Obtained liquid enzyme formulation is placed in 25 DEG C, and is spaced one month and detects its glutamine transaminage and protease
Enzyme activity storage rate (the enzyme activity * 100% measured when the enzyme activity/0h measured when enzyme activity storage rate=xth h), testing result such as table
5。
Such as table 5, in liquid enzyme formulation treated by the present method, the enzyme activity of glutamine transaminage and protease saves good
It is good, it is saved 8 months under room temperature, glutamine transaminage enzyme activity storage rate is up to 83%, and proteinase activity storage rate is up to 86%.
The enzyme activity storage rate of the enzyme in liquid enzyme formulation under the conditions of 25 DEG C of table 5
Comparative example 1: the preparation of liquid enzyme formulation
Specific step is as follows:
(1) glutamine transaminage is added in water to be mixed, obtaining glutamine transaminage enzyme activity is 500U/mL's
Glutamine transaminage aqueous solution；
(2) protease is added in water to be mixed, obtains the aqueous solution of protease that proteinase activity is 400U/mL；
(3) glutamine transaminage liquid preparation is mixed with liquid of protease body preparation according to the volume ratio of 10:1,
And the HCl solution that concentration is 1M is added and adjusts pH to 6, obtain liquid enzyme formulation.
By obtained liquid enzyme formulation in 25 DEG C place, and respectively at 0h, 12h, for 24 hours, 36h, 48h, 60h, 7d detection
Its glutamine transaminage and proteinase activity storage rate (measure when the enzyme activity/0h measured when enzyme activity storage rate=xth h
Enzyme activity * 100%), testing result such as table 6.
Such as table 6, liquid enzyme formulation is prepared if directly mixing glutamine transaminage and protease, between the two
It can consume mutually, so that enzyme preparation glutamine transaminase and the enzyme activity of protease are remarkably decreased.
The enzyme activity storage rate of the enzyme in liquid enzyme formulation under the conditions of 25 DEG C of table 6
Comparative example 2: the preparation of liquid enzyme formulation
Specific step is as follows:
(1) glutamine transaminage is added in water to be mixed, obtaining glutamine transaminage enzyme activity is 500U/mL's
Glutamine transaminage aqueous solution；
(2) protease is added in water to be mixed, obtains the aqueous solution of protease that proteinase activity is 400U/mL；
(3) in aqueous solution of protease be added account for aqueous solution of protease quality 20% glycerol, 4.5% chitosan,
0.8% casein, 0.5% sodium propionate, 5% maltodextrin stir 10min under 120r/min revolving speed, obtain albumen
Enzyme solution body preparation；
(4) glutamine transaminage liquid preparation is mixed with liquid of protease body preparation according to the volume ratio of 10:1,
And the HCl solution that concentration is 1M is added and adjusts pH to 6, obtain liquid enzyme formulation.
Obtained liquid enzyme formulation is placed in 25 DEG C, and is spaced one month and detects its glutamine transaminage and protease
Enzyme activity storage rate (the enzyme activity * 100% measured when the enzyme activity/0h measured when enzyme activity storage rate=xth h), testing result such as table
7。
Such as table 7, untreated glutamine transaminage preparation is mixed with the protease preparation handled through the method for the present invention
It is combined, the enzyme activity of glutamine transaminage is remarkably decreased, and the enzyme activity of protease saves good, it may be possible to which protease largely disappears
Glutamine transaminage is consumed.
The enzyme activity storage rate of the enzyme in liquid enzyme formulation under the conditions of 25 DEG C of table 7
Comparative example 3: the preparation of liquid enzyme formulation
Specific step is as follows:
(1) glutamine transaminage is added in water to be mixed, obtaining glutamine transaminage enzyme activity is 500U/mL's
Glutamine transaminage aqueous solution；
(2) in glutamine transaminage aqueous solution be added account for glutamine transaminage aqueous solution quality 8% starch,
3.5% pectin, 1% hydroxypropyl methylcellulose, 2% D-araboascorbic acid sodium, 1.5% soybean protein hydrolyate, 0.6%
Lecithin stirs 8min under 100r/min revolving speed, obtains glutamine transaminage liquid preparation；
(3) protease is added in water to be mixed, obtains the aqueous solution of protease that proteinase activity is 400U/mL；
(4) glutamine transaminage liquid preparation is mixed with liquid of protease body preparation according to the volume ratio of 10:1,
And the HCl solution that concentration is 1M is added and adjusts pH to 6, obtain liquid enzyme formulation.
Obtained liquid enzyme formulation is placed in 25 DEG C, and is spaced one month and detects its glutamine transaminage and protease
Enzyme activity storage rate (the enzyme activity * 100% measured when the enzyme activity/0h measured when enzyme activity storage rate=xth h), testing result such as table
8。
Such as table 8, untreated protease preparation is mixed with the glutamine transaminage preparation handled through the method for the present invention
It is combined, the enzyme activity of protease is remarkably decreased, and the enzyme activity of glutamine transaminage saves well, it may be possible to which glutamine turns
Adnosine deaminase acts on protease.
The enzyme activity storage rate of the enzyme in liquid enzyme formulation under the conditions of 25 DEG C of table 8
Comparative example 4: the preparation of liquid enzyme formulation
Specific step is as follows:
(1) glutamine transaminage is added in water to be mixed, obtaining glutamine transaminage enzyme activity is 500U/mL's
Glutamine transaminage aqueous solution；
(2) in glutamine transaminage aqueous solution be added account for glutamine transaminage aqueous solution quality 3.5% pectin,
1% hydroxypropyl methylcellulose, 2% D-araboascorbic acid sodium, 1.5% soybean protein hydrolyate, 0.6% lecithin, in
8min is stirred under 100r/min revolving speed, obtains glutamine transaminage liquid preparation；
(3) protease is added in water to be mixed, obtains the aqueous solution of protease that proteinase activity is 400U/mL；
(4) in aqueous solution of protease be added account for aqueous solution of protease quality 20% glycerol, 4.5% chitosan,
0.8% casein, 0.5% sodium propionate, 5% maltodextrin stir 10min under 120r/min revolving speed, obtain albumen
Enzyme solution body preparation；
(5) glutamine transaminage liquid preparation is mixed with liquid of protease body preparation according to the volume ratio of 10:1,
And the HCl solution that concentration is 1M is added and adjusts pH to 5.5, obtain liquid enzyme formulation.
Obtained liquid enzyme formulation is placed in 25 DEG C, and is spaced one month and detects its glutamine transaminage and protease
Enzyme activity storage rate (the enzyme activity * 100% measured when the enzyme activity/0h measured when enzyme activity storage rate=xth h), testing result such as table
9。
Such as table 9, in liquid enzyme formulation treated by the present method, the equal preservation effect of the enzyme activity of glutamine transaminage is general,
It is saved 8 months under room temperature, glutamine transaminage enzyme activity storage rate only has 28%, and the enzyme activity preservation effect of protease remains unchanged not
Difference has 76%.
The enzyme activity storage rate of the enzyme in liquid enzyme formulation under the conditions of 25 DEG C of table 9
Comparative example 5: the preparation of liquid enzyme formulation
Specific step is as follows:
(1) glutamine transaminage is added in water to be mixed, obtaining glutamine transaminage enzyme activity is 500U/mL's
Glutamine transaminage aqueous solution；
(2) in glutamine transaminage aqueous solution be added account for glutamine transaminage aqueous solution quality 8% starch,
3.5% pectin, 1% hydroxypropyl methylcellulose, 2% D-araboascorbic acid sodium, 1.5% soybean protein hydrolyate, 0.6%
Lecithin stirs 8min under 100r/min revolving speed, obtains glutamine transaminage liquid preparation；
(3) protease is added in water to be mixed, obtains the aqueous solution of protease that proteinase activity is 400U/mL；
(4) in aqueous solution of protease be added account for aqueous solution of protease quality 4.5% chitosan, 0.8% casein,
0.5% sodium propionate, 5% maltodextrin, stir 10min under 120r/min revolving speed, obtain liquid of protease body preparation；
(5) glutamine transaminage liquid preparation is mixed with liquid of protease body preparation according to the volume ratio of 10:1,
And the HCl solution that concentration is 1M is added and adjusts pH to 7, obtain liquid enzyme formulation.
Obtained liquid enzyme formulation is placed in 25 DEG C, and is spaced one month and detects its glutamine transaminage and protease
Enzyme activity storage rate (the enzyme activity * 100% measured when the enzyme activity/0h measured when enzyme activity storage rate=xth h), testing result such as table
10。
Such as table 10, in liquid enzyme formulation treated by the present method, the enzyme activity of glutamine transaminage and protease is saved
Effect is general, saves 8 months under room temperature, and glutamine transaminage enzyme activity storage rate only has 74%, and protease only has 48%.
The enzyme activity storage rate of the enzyme in liquid enzyme formulation under the conditions of 25 DEG C of table 10
Comparative example 6: the preparation of liquid enzyme formulation
Specific step is as follows:
(1) glutamine transaminage is added in water to be mixed, obtaining glutamine transaminage enzyme activity is 500U/mL's
Glutamine transaminage aqueous solution；
(2) in glutamine transaminage aqueous solution be added account for glutamine transaminage aqueous solution quality 8% starch,
1% hydroxypropyl methylcellulose, 2% D-araboascorbic acid sodium, 1.5% soybean protein hydrolyate, 0.6% lecithin, in
8min is stirred under 100r/min revolving speed, obtains glutamine transaminage liquid preparation；
(3) protease is added in water to be mixed, obtains the aqueous solution of protease that proteinase activity is 400U/mL；
(4) in aqueous solution of protease be added account for aqueous solution of protease quality 20% glycerol, 4.5% chitosan,
0.5% sodium propionate, 5% maltodextrin, stir 10min under 120r/min revolving speed, obtain liquid of protease body preparation；
(5) glutamine transaminage liquid preparation is mixed with liquid of protease body preparation according to the volume ratio of 10:1,
And the HCl solution that concentration is 1M is added and adjusts pH to 6, obtain liquid enzyme formulation.
Obtained liquid enzyme formulation is placed in 25 DEG C, and is spaced one month and detects its glutamine transaminage and protease
Enzyme activity storage rate (the enzyme activity * 100% measured when the enzyme activity/0h measured when enzyme activity storage rate=xth h), testing result such as table
11。
Such as table 11, in liquid enzyme formulation treated by the present method, the enzyme activity of glutamine transaminage and protease is saved
Effect is general, saves 8 months under room temperature, and glutamine transaminage enzyme activity storage rate only has 55%, and protease only has 61%.
The enzyme activity storage rate of the enzyme in liquid enzyme formulation under the conditions of 25 DEG C of table 11
Comparative example 7: the preparation of liquid enzyme formulation
Specific step is as follows:
(1) glutamine transaminage is added in water to be mixed, obtaining glutamine transaminage enzyme activity is 500U/mL's
Glutamine transaminage aqueous solution；
(2) in glutamine transaminage aqueous solution be added account for glutamine transaminage aqueous solution quality 8% starch,
3.5% pectin, 2% D-araboascorbic acid sodium, 1.5% soybean protein hydrolyate, 0.6% lecithin, in 100r/min
8min is stirred under revolving speed, obtains glutamine transaminage liquid preparation；
(3) protease is added in water to be mixed, obtains the aqueous solution of protease that proteinase activity is 400U/mL；
(4) in aqueous solution of protease be added account for aqueous solution of protease quality 20% glycerol, 0.8% casein,
0.5% sodium propionate, 5% maltodextrin, stir 10min under 120r/min revolving speed, obtain liquid of protease body preparation；
(5) glutamine transaminage liquid preparation is mixed with liquid of protease body preparation according to the volume ratio of 10:1,
And the HCl solution that concentration is 1M is added and adjusts pH to 6, obtain liquid enzyme formulation.
Obtained liquid enzyme formulation is placed in 25 DEG C, and is spaced one month and detects its glutamine transaminage and protease
Enzyme activity storage rate (the enzyme activity * 100% measured when the enzyme activity/0h measured when enzyme activity storage rate=xth h), testing result such as table
12。
Such as table 12, in liquid enzyme formulation treated by the present method, the enzyme activity of glutamine transaminage and protease is saved
Effect is general, saves 8 months under room temperature, and glutamine transaminage enzyme activity storage rate only has 57%, and protease only has 65%.
The enzyme activity storage rate of the enzyme in liquid enzyme formulation under the conditions of 25 DEG C of table 12
Comparative example 8: the preparation of liquid enzyme formulation
Specific step is as follows:
(1) glutamine transaminage is added in water to be mixed, obtaining glutamine transaminage enzyme activity is 500U/mL's
Glutamine transaminage aqueous solution；
(2) in glutamine transaminage aqueous solution be added account for glutamine transaminage aqueous solution quality 8% starch,
3.5% pectin, 1% hydroxypropyl methylcellulose, 2% D-araboascorbic acid sodium, 0.6% lecithin turn in 100r/min
Speed is lower to stir 8min, obtains glutamine transaminage liquid preparation；
(3) protease is added in water to be mixed, obtains the aqueous solution of protease that proteinase activity is 400U/mL；
(4) in aqueous solution of protease be added account for aqueous solution of protease quality 20% glycerol, 4.5% chitosan,
0.8% casein, 0.5% sodium propionate, stir 10min under 120r/min revolving speed, obtain liquid of protease body preparation；
(5) glutamine transaminage liquid preparation is mixed with liquid of protease body preparation according to the volume ratio of 10:1,
And the HCl solution that concentration is 1M is added and adjusts pH to 6, obtain liquid enzyme formulation.
Obtained liquid enzyme formulation is placed in 25 DEG C, and is spaced one month and detects its glutamine transaminage and protease
Enzyme activity storage rate (the enzyme activity * 100% measured when the enzyme activity/0h measured when enzyme activity storage rate=xth h), testing result such as table
13。
Such as table 13, in liquid enzyme formulation treated by the present method, the enzyme activity of glutamine transaminage and protease is saved
Effect is general, saves 8 months under room temperature, and glutamine transaminage enzyme activity storage rate only has 54%, and protease only has 70%.
The enzyme activity storage rate of the enzyme in liquid enzyme formulation under the conditions of 25 DEG C of table 13
Comparative example 9: the preparation of liquid enzyme formulation
Specific step is as follows:
(1) glutamine transaminage is added in water to be mixed, obtaining glutamine transaminage enzyme activity is 500U/mL's
Glutamine transaminage aqueous solution；
(2) in glutamine transaminage aqueous solution be added account for glutamine transaminage aqueous solution quality 8% starch,
3.5% pectin, 1% hydroxypropyl methylcellulose, 2% D-araboascorbic acid sodium, 1.5% soybean protein hydrolyate, in 100r/
8min is stirred under min revolving speed, obtains glutamine transaminage liquid preparation；
(3) protease is added in water to be mixed, obtains the aqueous solution of protease that proteinase activity is 400U/mL；
(4) in aqueous solution of protease be added account for aqueous solution of protease quality 20% glycerol, 4.5% chitosan,
0.8% casein, 5% maltodextrin, stir 10min under 120r/min revolving speed, obtain liquid of protease body preparation；
(5) glutamine transaminage liquid preparation is mixed with liquid of protease body preparation according to the volume ratio of 10:1,
And the HCl solution that concentration is 1M is added and adjusts pH to 6, obtain liquid enzyme formulation.
Obtained liquid enzyme formulation is placed in 25 DEG C, and is spaced one month and detects its glutamine transaminage and protease
Enzyme activity storage rate (the enzyme activity * 100% measured when the enzyme activity/0h measured when enzyme activity storage rate=xth h), testing result such as table
14。
Such as table 14, in liquid enzyme formulation treated by the present method, the enzyme activity of glutamine transaminage and protease is saved
Effect is general, saves 8 months under room temperature, and glutamine transaminage enzyme activity storage rate only has 48%, and protease only has 59%.
The enzyme activity storage rate of the enzyme in liquid enzyme formulation under the conditions of 25 DEG C of table 14
Embodiment 6: the application of liquid enzyme formulation
Specific step is as follows:
The beef of 15 pieces of 150*50*50mm is taken, the liquid enzyme formulation for first respectively obtaining 5mL physiological saline, embodiment 1-5
And the liquid enzyme formulation that comparative example 1-9 is obtained injects beef, then respectively obtains 5mL physiological saline, embodiment 1-5
Liquid enzyme formulation and the obtained liquid enzyme formulation of comparative example 1-9 be applied to beef surface；The beef elder generation tumbling that will be handled well
1h, then stand 2h.
Moisture is carried out to the beef handled well, is that waterpower, water activity, shearing force and texture intensity, testing result are shown in Table
15。
Such as table 15, it is strong that the liquid enzyme formulation of embodiment 1-5 can obviously improve the water activity of beef, shearing force and texture
Degree, compared with blank control, can improve the water activity of beef to 0.89Aw/% from 0.81Aw/%, shearing force is reduced to from 38N
15N, texture intensity is from 18g/mm2It improves to 42g/mm2；And comparative example is ineffective.
Influence of the 15 different liquids enzyme preparation of table to beef quality
| Water activity | Shearing force | Texture intensity | |
| Embodiment 1 | 0.89Aw/% | 16N | 42g/mm2 |
| Embodiment 2 | 0.86Aw/% | 19N | 36g/mm2 |
| Embodiment 3 | 0.87Aw/% | 18N | 39g/mm2 |
| Embodiment 4 | 0.84Aw/% | 21N | 33g/mm2 |
| Embodiment 5 | 0.85Aw/% | 21N | 32g/mm2 |
| Comparative example 1 | 0.80Aw/% | 32N | 22g/mm2 |
| Comparative example 2 | 0.80Aw/% | 25N | 17g/mm2 |
| Comparative example 3 | 0.83Aw/% | 31N | 28g/mm2 |
| Comparative example 4 | 0.81Aw/% | 27N | 24g/mm2 |
| Comparative example 5 | 0.83Aw/% | 32N | 29g/mm2 |
| Comparative example 6 | 0.82Aw/% | 27N | 26g/mm2 |
| Comparative example 7 | 0.82Aw/% | 26N | 27g/mm2 |
| Comparative example 8 | 0.82Aw/% | 24N | 27g/mm2 |
| Comparative example 9 | 0.81Aw/% | 27N | 26g/mm2 |
| Blank control | 0.79Aw/% | 38N | 22g/mm2 |
Although the present invention has been described by way of example and in terms of the preferred embodiments, it is not intended to limit the invention, any to be familiar with this skill
The people of art can do various change and modification, therefore protection model of the invention without departing from the spirit and scope of the present invention
Enclosing subject to the definition of the claims.
Claims (9)
1. a kind of liquid enzyme formulation, which is characterized in that the ingredient of the liquid enzyme formulation includes glutamine transaminage liquid system
Agent and liquid of protease body preparation；
The ingredient of the glutamine transaminage liquid preparation includes the paddy that glutamine transaminage enzyme activity is 10~1000U/mL
Glutamine transaminase aqueous solution and account for 5~10% starch of glutamine transaminage aqueous solution quality, 3~5% pectin,
0.1~3% hydroxypropyl methylcellulose, 1~5% D-araboascorbic acid sodium, 0.5~2% soybean protein hydrolyate, 0.5~1%
Lecithin；
The ingredient of the liquid of protease body preparation includes the aqueous solution of protease and account for that proteinase activity is 10~1000U/mL
The glycerol of aqueous solution of protease quality 15~30%, 3~5% chitosan, 0.1~1% casein, 0.1~1% propionic acid
Sodium, 3~8% maltodextrin.
2. a kind of liquid enzyme formulation as described in claim 1, which is characterized in that the glutamine transaminage liquid preparation
Ingredient includes the glutamine transaminage aqueous solution and account for glutamine transaminage that glutamine transaminage enzyme activity is 500U/mL
The starch of aqueous solution quality 8%, 3.5% pectin, 1% hydroxypropyl methylcellulose, 2% D-araboascorbic acid sodium, 1.5% big
Legumin hydrolysate, 0.6% lecithin；
The ingredient of the liquid of protease body preparation includes the aqueous solution of protease and account for protease that proteinase activity is 400U/mL
The glycerol of aqueous solution quality 20%, 4.5% chitosan, 0.8% casein, 0.5% sodium propionate, 5% maltodextrin.
3. a kind of liquid enzyme formulation as claimed in claim 1 or 2, which is characterized in that the liquid enzyme formulation glutamine
The volume ratio of transaminase liquid preparation and liquid of protease body preparation is 5~30:1.
4. a kind of liquid enzyme formulation a method according to any one of claims 1-3, which is characterized in that the pH of the liquid enzyme formulation is 5
~7.
5. a kind of preparation method of liquid enzyme formulation as described in claim 1-4 is any, which is characterized in that the method be
Glutamine transaminage is added in water to be mixed, the glutamine that glutamine transaminage enzyme activity is 10~1000U/mL is obtained
Transaminase aqueous solution；It is added in glutamine transaminage aqueous solution and accounts for glutamine transaminage aqueous solution quality 5~10%
Starch, 3~5% pectin, 0.1~3% hydroxypropyl methylcellulose, 1~5% D-araboascorbic acid sodium, 0.5~2% soybean
Protolysate, 0.5~1% lecithin, under 80~150r/min revolving speed stir 5~10min, obtain glutamine and turn ammonia
Enzyme solution body preparation；
Protease is added in water to be mixed, the aqueous solution of protease that proteinase activity is 10~1000U/mL is obtained；In egg
Be added in white enzyme aqueous solution the glycerol, 3~5% that account for aqueous solution of protease quality 15~30% chitosan, 0.1~1% it is dry
Casein, 0.1~1% sodium propionate, 3~8% maltodextrin stir 5~10min under 80~150r/min revolving speed, obtain
Liquid of protease body preparation；
Glutamine transaminage liquid preparation is mixed with liquid of protease body preparation according to certain volume ratio, liquid is obtained
Enzyme preparation.
6. a kind of preparation method of liquid enzyme formulation as claimed in claim 5, which is characterized in that the method is to add in water
Enter glutamine transaminage to be mixed, it is water-soluble to obtain the glutamine transaminage that glutamine transaminage enzyme activity is 500U/mL
Liquid；It is added in glutamine transaminage aqueous solution and accounts for 8% starch of glutamine transaminage aqueous solution quality, 3.5% fruit
Glue, 1% hydroxypropyl methylcellulose, 2% D-araboascorbic acid sodium, 1.5% soybean protein hydrolyate, 0.6% lecithin, in
8min is stirred under 100r/min revolving speed, obtains glutamine transaminage liquid preparation；
Protease is added in water to be mixed, the aqueous solution of protease that proteinase activity is 400U/mL is obtained；In protease water
Be added in solution account for the glycerol of aqueous solution of protease quality 20%, 4.5% chitosan, 0.8% casein, 0.5% third
Sour sodium, 5% maltodextrin, stir 10min under 120r/min revolving speed, obtain liquid of protease body preparation.
7. such as a kind of preparation method of liquid enzyme formulation described in claim 5 or 6, which is characterized in that the liquid enzyme formulation
The volume ratio of glutamine transaminase liquid preparation and liquid of protease body preparation is 5~30:1.
8. a kind of preparation method of liquid enzyme formulation as described in claim 5-7 is any, which is characterized in that the liquid enzymes system
The pH of agent is 5~7.
9. a kind of any described liquid enzyme formulation of claim 1-4 or a kind of any liquid enzymes system of claim 5-7
Application of the preparation method of agent in terms of preparing food, drug, health care product.
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| CN115968925A (en) * | 2023-02-13 | 2023-04-18 | 中山市南方新元食品生物工程有限公司 | Chongqing special enzyme preparation for noodles and preparation method thereof |
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