CN109342386A - A kind of quantum dot fluorescence detection device and method - Google Patents
A kind of quantum dot fluorescence detection device and method Download PDFInfo
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- CN109342386A CN109342386A CN201811604821.0A CN201811604821A CN109342386A CN 109342386 A CN109342386 A CN 109342386A CN 201811604821 A CN201811604821 A CN 201811604821A CN 109342386 A CN109342386 A CN 109342386A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6402—Atomic fluorescence; Laser induced fluorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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Abstract
The invention discloses a kind of quantum dot fluorescence detection device and methods, belong to fluorescence detection field, including test desk, the lower end four corners of test desk are fixedly connected to support column, the upper end of test desk is fixedly connected with a pair of of support base, the upper end of a pair of of support base is fixedly connected with attachment base, the left end of one support base is fixedly connected with master controller, the lower end of attachment base is fixedly connected sequentially from left to right the first cylinder and the second cylinder, the lower end of first cylinder and the second cylinder has been respectively fixedly connected with laser emitter and transmission electron microscope, the upper end of test desk is fixedly connected sequentially from left to right has four sides to lead to quartz cuvette pond and a pair of of support leg, the upper end of a pair of of support leg is fixedly connected with copper mesh, the upper end of test desk is fixedly connected sequentially from left to right the first mounting blocks, second mounting blocks and third mounting blocks, based on fluorescence detection On the basis of superiority, dopamine detection method a kind of simple, quick, sensitive and that selectivity is good is established.
Description
Technical field
The present invention relates to fluorescence detection fields, more specifically to a kind of quantum dot fluorescence detection device and method.
Background technique
In recent years, quantum dot is mentioned as the quick measurement that a kind of novel semiconductor nano luminescent material is various analytes
Supplied may, compared with traditional organic dyestuff, quantum dot due to high with quantum yield, photostability is strong, launch wavelength can
It adjusts, bleach-resistant ability is strong and is easy to the characteristics such as modification and is widely used in inorganic ions, small organic molecule and large biological molecule
Sensitive Detection, the quantum dot being widely used at present mainly has CdTe, the monokaryons type quantum dot such as CdSe, CdS, but monokaryon type quantum
Point there are surface defects it is more, fluorescence efficiency is lower, the stability of photooxidation is poor the deficiencies of, recent studies have shown that, in list
On the basis of caryogram quantum dot further after quantum dot coats and to form core-shell type quantum point, monokaryon type quantum dot can be effectively overcome
Deficiency, and optical property is more stable, can effectively increase the sensitivity to analysis analyte detection.
Dopamine as neurotransmitter important in mammalian central nervous system, maintain with adjust nervous system,
Cardiovascular system, renal function and hormonal secretion etc. play a significant role, the variation of machine intracorporal concentration with
The generation of many diseases is closely related, and internal dopamine secretion imbalance is to lead to neuromuscular disorders, schizophrenia, Parkinson
The major reason that a variety of mental disorders such as disease, Alzheimer's disease occur, internal DOPAMINE CONTENT IN RABBIT are also clinically spiritual
The important indicator of class disease detection, as a kind of cardiovascular drugs, dopamine have enhancing myocardial contraction, expansion visceral vessel,
The function of increasing renal blood flow is usually used in myocardial infarction, renal failure, bronchial asthma and blood loss, cardiogenic and sense
The treatment of metachromia shock, because dopamine directly affects in machine intracorporal concentration variation the cerebration of human body, drug injection with
Strict control dosage is not only needed when taking, and needs an accurate quantitative detecting method, currently, reported dopamine
Detection method mainly includes capillary electrophoresis analysis method, electrochemical methods, colorimetric analysis and flow injection analysis etc.,
In, fluorescence detection is received significant attention since simple and quick and detection limit is low, but existing fluorescence detection superiority
Poor, higher cost, operating procedure is relatively complicated, therefore the superiority based on fluorescence detection, explores a kind of simple and quick, clever
Quick and at low cost dopamine detection method is of great significance.
Summary of the invention
1. technical problems to be solved
Aiming at the problems existing in the prior art, the purpose of the present invention is to provide a kind of quantum dot fluorescence detection device and
Method, it is established a kind of simple, quick, sensitive and good selectivity more on the basis of the superiority based on fluorescence detection
Bar amine detection method.
2. technical solution
To solve the above problems, the present invention adopts the following technical scheme that.
A kind of quantum dot fluorescence detection device and method, including test desk, the lower end four corners of the test desk are fixed
It is connected with support column, the upper end of the test desk is fixedly connected with a pair of of support base, and the upper end of a pair of support base is fixed to be connected
Be connected to attachment base, the left end of a support base is fixedly connected with master controller, the lower end of the attachment base from left to right according to
Secondary to be fixedly connected with the first cylinder and the second cylinder, the lower end of first cylinder and the second cylinder has been respectively fixedly connected with laser
Transmitter and transmission electron microscope, the upper end of the test desk is fixedly connected sequentially from left to right has four sides to lead to quartz cuvette pond and a pair
Support leg, the four sides leads to quartz cuvette pond and a pair of of support leg is respectively positioned between a pair of of support base, a pair of support leg
Upper end is fixedly connected with copper mesh, and the four sides, which leads to, is filled with aqueous solution in quartz cuvette pond, the four sides leads to quartz cuvette pond position
In the downside of laser emitter, the copper mesh is located at the downside of transmission electron microscope, and the upper end of the test desk is successively solid from left to right
Surely the first mounting blocks, the second mounting blocks and third mounting blocks are connected with, first mounting blocks are located at the right side of a pair of of support base,
The upper end of first mounting blocks, which is dug, multiple equally distributed first mounting grooves, and the inner end of first mounting groove is plugged with
Sepectrophotofluorometer, the upper end of second mounting blocks, which is dug, the second mounting groove, the inner end grafting of second mounting groove
Have a suction pipe, the upper end of the third mounting blocks, which is dug, multiple equally distributed third mounting grooves, the third mounting groove it is interior
End is plugged with colorimetric cylinder, on the basis of the superiority based on fluorescence detection, establishes a kind of simple, quick, sensitive and selects
The good dopamine detection method of property.
Further, the surface thermoprint of the colorimetric cylinder has black containing mark scale, the black containing mark scale
Lower limit be 0mL, the upper limit of the black containing mark scale is 10mL, and the capacity of the colorimetric cylinder is 10mL.
Further, the inner end of multiple first mounting grooves, the second mounting groove and multiple third mounting grooves passes through Buddhist nun
Dragon patch is bonded with foam-rubber cushion, and foam-rubber cushion has good protective effect, and nylon tape is easily installed foam-rubber cushion.
Further, the support column is coated with resilient cushion, and the resilient cushion is coated with silencing pad, institute
State silencing pad surface be coated with antistatic japanning, resilient cushion have good cushioning effect, silencing pad can effective noise reduction function, prevent
Electrostatic convergence painting has good anti-static effect.
Further, the master controller and external power supply are electrically connected, first cylinder, laser emitter and second
Cylinder is electrically connected at master controller, and the transmission electron microscope is electrically connected with 200kV high speed voltage source.
Further, the model Cary Eclipse sepectrophotofluorometer of the sepectrophotofluorometer, through ability
Field technique personnel it is on probation after, the sepectrophotofluorometer better effect of the model.
Further, detection method are as follows:
S1, the exterior appearance for obtaining CdSe/ZnS quantum dot is scanned under 200kV high speed voltage by transmission electron microscope, by it
Merging four sides leads in quartz cuvette pond, and CdSe/ZnS quantum dot aqueous solution is made；
S2, the first cylinder is started by control master controller forward direction, extends the length of the first cylinder, makes laser emitter
Output end protrudes into CdSe/ZnS quantum dot aqueous solution, the first cylinder is closed by control master controller, by controlling main control
Device starts laser emitter, ultrasonic 20min, through taking out suction pipe in the second mounting groove, by the CdSe/ZnS quantum dot after ultrasound
Aqueous solution draws several drops, is then scattered in the surface of copper mesh (12), through the dry 60min of natural cooling；
S3, the second cylinder is started by control master controller forward direction, the length of the second cylinder is extended, so that transmission electron microscope position
When close to the upside of copper mesh, the second cylinder is closed by control master controller, starting transmission electron microscope makes transmission electron microscope to filling
CdSe/ZnS quantum dot after dividing drying is measured；
S4, dopamine and water are taken, dopamine and water is made into the standard solution of final concentration of 10 μm of ol/L, installed through third
Colorimetric cylinder is taken out in slot, sequentially add 0.5mL concentration be 2.0 × 105 μm of ol/L phosphate buffer (PH6.5-8.0),
0.01mL concentration is the CdSe/ZnS quantum dot solution of 0.08 μm of ol/L, is settled to 5mL with water, shakes up, reacts at room temperature
After 20min, through taking out sepectrophotofluorometer in the first mounting groove, using sepectrophotofluorometer to a progress fluorescence detection.
Further, the pH value of the phosphate buffer is 7.35, and the pH value of the phosphate buffer takes PHS-3C type
PH meter is measured, through our company technical operation personnel's thousands of times experiment after, after measured pH value be 7.35 phosphate buffer effect
Most preferably, PHS-3C type pH meter measurement accuracy is high.
3. beneficial effect
Compared with the prior art, the present invention has the advantages that
(1) present invention is established a kind of simple, quick, sensitive and is selected on the basis of the superiority based on fluorescence detection
The good dopamine detection method of selecting property.
(2) inner end of multiple first mounting grooves, the second mounting groove and multiple third mounting grooves is bonded with by nylon tape
Foam-rubber cushion, foam-rubber cushion have good protective effect, and nylon tape is easily installed foam-rubber cushion.
(3) support column is coated with resilient cushion, and resilient cushion is coated with silencing pad, the surface of silencing pad is coated with
Antistatic japanning, resilient cushion have good cushioning effect, silencing pad can effective noise reduction function, antistatic japanning have it is good
Anti-static effect.
(4) the model Cary Eclipse sepectrophotofluorometer of sepectrophotofluorometer, through those skilled in the art
It is on probation after, the sepectrophotofluorometer better effect of the model.
(5) pH value of phosphate buffer is 7.35, and the pH value of phosphate buffer takes PHS-3C type pH meter to be measured,
After the experiment of our company technical operation personnel's thousands of times, while convenient for measurement, the phosphate buffer effect that pH value is 7.35 after measured
Most preferably, PHS-3C type pH meter measurement accuracy is high.
Detailed description of the invention
Fig. 1 is the structural diagram of the present invention；
Fig. 2 is the structural schematic diagram in Fig. 1 at A；
Fig. 3 is the transmission electron microscope picture of CdSe/ZnS quantum dot of the invention；
Fig. 4 is excitation and launching light spectrogram of the invention；
Fig. 5 is influence lab diagram of the various concentration dopamine to CdSe/ZnS quantum dot fluorescence emission spectrum.
Figure label explanation:
1 test desk, 2 support columns, 3 support bases, 4 attachment bases, 5 first cylinders, 6 laser emitters, 7 second cylinders, 8 transmissions
Electronic Speculum, 9 master controllers, 10 four sides lead to quartz cuvette pond, 11 support legs, 12 copper mesh, 13 first mounting blocks, 1,301 first installations
Slot, 1302 sepectrophotofluorometers, 14 second mounting blocks, 1,401 second mounting grooves, 1402 suction pipes, 15 third mounting blocks, 1501
Third mounting groove, 1502 colorimetric cylinders.
Specific embodiment
Technical solution in the embodiment of the present invention that following will be combined with the drawings in the embodiments of the present invention carries out clear, complete
Ground description.Obviously, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments, based on this
Embodiment in invention, every other reality obtained by those of ordinary skill in the art without making creative efforts
Example is applied, shall fall within the protection scope of the present invention.
In the description of the present invention, it should be noted that the instruction such as term " on ", "lower", "inner", "outside" " top/bottom end "
Orientation or positional relationship be based on the orientation or positional relationship shown in the drawings, be merely for convenience of description the present invention and simplification retouch
It states, rather than the device or element of indication or suggestion meaning must have a particular orientation, be constructed and operated in a specific orientation,
Therefore it is not considered as limiting the invention.In addition, term " first ", " second " are used for description purposes only, and cannot understand
For indication or suggestion relative importance.
In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, term " installation " " is set
Be equipped with ", " be arranged/connect ", " connection " etc., shall be understood in a broad sense, such as " connection ", may be a fixed connection, be also possible to removable
Connection is unloaded, or is integrally connected；It can be mechanical connection, be also possible to be electrically connected；It can be directly connected, it can also be in
Between medium be indirectly connected, can be the connection inside two elements.It for the ordinary skill in the art, can be specific
Situation understands the concrete meaning of above-mentioned term in the present invention.
Embodiment:
Please refer to Fig. 1 and Fig. 2, a kind of quantum dot fluorescence detection device and method, including test desk 1, the lower end of test desk 1
Four corners are fixedly connected to support column 2, and support column 2 plays support, and the upper end of test desk 1 is fixedly connected with a pair of of support
Seat 3, a pair of of support base 3 plays a part of to support and fix, and the upper end of a pair of of support base 3 is fixedly connected with attachment base 4, attachment base 4
Play a part of fixed and connect, the left end of a support base 3 is fixedly connected with master controller 9, and master controller 9 plays the work of control
With the lower end of attachment base 4 is fixedly connected sequentially from left to right the first cylinder 5 and the second cylinder 7, the first cylinder 5 and the second gas
Cylinder 7 can be extended and be shortened, and the lower end of the first cylinder 5 and the second cylinder 7 has been respectively fixedly connected with laser emitter 6 and transmission
Electronic Speculum 8, transmitting of the laser emitter 6 for ultrasound, transmission electron microscope 8 are used to that CdSe/ZnS quantum dot to be made, while can be to CdSe/
ZnS quantum dot carries out relevant detection, and the upper end of test desk 1 is fixedly connected sequentially from left to right has four sides to lead to quartz cuvette pond 10
With a pair of of support leg 11, four sides leads to quartz cuvette pond 10 and leads to quartz cuvette for CdSe/ZnS quantum dot aqueous solution, four sides to be made
Pond 10 and a pair of of support leg 11 are respectively positioned between a pair of of support base 3, and the upper end of a pair of of support leg 11 is fixedly connected with copper mesh 12, and four
Face, which leads to, is filled with aqueous solution in quartz cuvette pond 10, four sides leads to the downside that quartz cuvette pond 10 is located at laser emitter 6, copper mesh 12
Positioned at the downside of transmission electron microscope 8, the upper end of test desk 1 is fixedly connected sequentially from left to right the installation of the first mounting blocks 13, second
Block 14 and third mounting blocks 15, the first mounting blocks 13 are located at the right side of a pair of of support base 3, and the upper end of the first mounting blocks 13, which is dug, to be had
Multiple equally distributed first mounting grooves 1301, the inner end of the first mounting groove 1301 is plugged with sepectrophotofluorometer 1302, glimmering
Light spectrophotometer 1302 is used for the analysis of fluorescence emission spectrum, and the upper end of the second mounting blocks 14, which is dug, the second mounting groove
1401, the inner end of the second mounting groove 1401 is plugged with suction pipe 1402, and suction pipe 1402 takes CdSe/ZnS quantum dot aqueous solution for dripping,
The upper end of third mounting blocks 15, which is dug, multiple equally distributed third mounting grooves 1501, the inner end grafting of third mounting groove 1501
There is colorimetric cylinder 1502, colorimetric cylinder 1502 on the basis of the superiority based on fluorescence detection, establishes one for constant volume liquid to be made
The dopamine detection method that kind is simple, quick, sensitive and selectivity is good.
Further, the surface thermoprint of colorimetric cylinder 1502 has a black containing mark scale, under black containing mark scale
It is limited to 0mL, the upper limit of black containing mark scale is 10mL, and the capacity of colorimetric cylinder 1502 is 10mL.
Further, the inner end of multiple first mounting grooves 1301, the second mounting groove 1401 and multiple third mounting grooves 1501
Foam-rubber cushion is bonded with by nylon tape, foam-rubber cushion has good protective effect, and nylon tape is easily installed foam-rubber cushion.
Further, support column 2 is coated with resilient cushion, and resilient cushion is coated with silencing pad, silencing pad
Surface be coated with antistatic japanning, resilient cushion have good cushioning effect, silencing pad can effective noise reduction function, antistatic japanning tool
There is good anti-static effect.
Further, master controller 9 and external power supply are electrically connected, the first cylinder 5, laser emitter 6 and the second cylinder 7
It is electrically connected at master controller 9, transmission electron microscope 8 is electrically connected with 200kV high speed voltage source.
Further, the model Cary Eclipse sepectrophotofluorometer of sepectrophotofluorometer 1302, through ability
Field technique personnel it is on probation after, 1302 better effect of sepectrophotofluorometer of the model.
Further, detection method are as follows:
S1, the exterior appearance for obtaining CdSe/ZnS quantum dot is scanned under 200kV high speed voltage by transmission electron microscope 8, it will
It is placed in four sides and leads in quartz cuvette pond 10, and CdSe/ZnS quantum dot aqueous solution is made；
S2, start the first cylinder 5 by the way that control master controller 9 is positive, extend the length of the first cylinder 5, make Laser emission
The output end of device 6 protrudes into CdSe/ZnS quantum dot aqueous solution, closes the first cylinder 5 by control master controller 9, passes through control
Master controller 9 starts laser emitter 6, ultrasonic 20min, through taking out suction pipe 1402 in the second mounting groove 1401, after ultrasound
CdSe/ZnS quantum dot aqueous solution draw several drops, be then scattered in the surface of copper mesh 12, it is dry through natural cooling
60min；
S3, pass through 9 the second cylinder 7 of positive starting of control master controller, the length of the second cylinder 7 of elongation, so that transmission electricity
When mirror 8 is located at close to the upside of copper mesh 12, the second cylinder 7 is closed by control master controller 9, starting transmission electron microscope 8 makes
Radio mirror 8 is measured the CdSe/ZnS quantum dot after abundant drying, and specific measurement result is detailed in Fig. 3 and Fig. 4, by Fig. 3
It can be seen that with Fig. 4, quantum dot particle is similar to ball-type, and size uniformity, overall scatter is good, and partial size is about 5nm；Quantum dot
Maximum excitation wavelength be located at 300nm (curve a), and maximum emission wavelength be located at 595nm (curve b), illustrate quantum dot send out
Optical property is good；
S4, dopamine and water are taken, dopamine and water is made into the standard solution of final concentration of 10 μm of ol/L, installed through third
Colorimetric cylinder 1502 is taken out in slot 1501, sequentially adds the phosphate buffer (PH6.5- that 0.5mL concentration is 2.0 × 105 μm of ol/L
8.0), 0.01mL concentration is the CdSe/ZnS quantum dot solution of 0.08 μm of ol/L, and the phosphoric acid buffer of various concentration is used through our company
After liquid experiment, referring specifically to Fig. 5, the results show that phosphate buffer, in pH5.5-6.5, dopamine is to CdSe/ZnS quantum
The fluorescent quenching intensity of point is remarkably reinforced with the increase of solution ph, and when between pH6.5-8.0, quenching intensity is relatively steady
Fixed, for the ease of measurement, it is the phosphate buffer (PH6.5-8.0) of 2.0 × 105 μm of ol/L that the present invention, which takes 0.5mL concentration,
Then it is settled to 5mL with water, shaken up, after reacting 20min at room temperature, through taking out fluorescence spectrophotometry in the first mounting groove 1301
Meter 1302, using sepectrophotofluorometer 1302 to a progress fluorescence detection.
Further, the pH value of phosphate buffer is 7.35, and the pH value of phosphate buffer takes PHS-3C type pH meter to carry out
Measurement, after the experiment of our company technical operation personnel's thousands of times, the phosphate buffer effect that pH value is 7.35 after measured is best,
PHS-3C type pH meter measurement accuracy is high.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
It improves design and is subject to equivalent substitution or change, should be covered by the scope of protection of the present invention.
Claims (8)
1. a kind of quantum dot fluorescence detection device, including test desk (1), which is characterized in that the lower end quadrangle of the test desk (1)
Place is fixedly connected to support column (2), and the upper end of the test desk (1) is fixedly connected with a pair of of support base (3), a pair of branch
The upper end of support seat (3) is fixedly connected with attachment base (4), and the left end of a support base (3) is fixedly connected with master controller
(9), the lower end of the attachment base (4) is fixedly connected sequentially from left to right the first cylinder (5) and the second cylinder (7), and described
The lower end of one cylinder (5) and the second cylinder (7) has been respectively fixedly connected with laser emitter (6) and transmission electron microscope (8), the measurement
The upper end of platform (1) is fixedly connected sequentially from left to right four sides logical quartz cuvette pond (10) and a pair of of support leg (11), and described four
Face leads to quartz cuvette pond (10) and a pair of of support leg (11) is respectively positioned between a pair of of support base (3), a pair of support leg (11)
Upper end be fixedly connected with copper mesh (12), the four sides lead to quartz cuvette pond (10) in be filled with aqueous solution, the four sides leads to stone
English colorimetric pool (10) is located at the downside of laser emitter (6), and the copper mesh (12) is located at the downside of transmission electron microscope (8), the survey
The upper end of amount platform (1) is fixedly connected sequentially has the first mounting blocks (13), the second mounting blocks (14) and third mounting blocks from left to right
(15), first mounting blocks (13) are located at the right side of a pair of of support base (3), and the upper end of first mounting blocks (13), which is dug, to be had
Multiple equally distributed first mounting grooves (1301), the inner end of first mounting groove (1301) are plugged with sepectrophotofluorometer
(1302), the upper end of second mounting blocks (14) is dug and is had the second mounting groove (1401), second mounting groove (1401)
Inner end is plugged with suction pipe (1402), and the upper end of the third mounting blocks (15), which is dug, multiple equally distributed third mounting grooves
(1501), the inner end of the third mounting groove (1501) is plugged with colorimetric cylinder (1502).
2. a kind of quantum dot fluorescence detection device according to claim 1, which is characterized in that the colorimetric cylinder (1502)
Surface thermoprint has black containing mark scale, and the lower limit of the black containing mark scale is 0mL, the black containing mark mark
The upper limit of ruler is 10mL, and the capacity of the colorimetric cylinder (1502) is 10mL.
3. a kind of quantum dot fluorescence detection device according to claim 1, which is characterized in that multiple first mounting grooves
(1301), the inner end of the second mounting groove (1401) and multiple third mounting grooves (1501) passes through nylon tape and is bonded with foam-rubber cushion.
4. a kind of quantum dot fluorescence detection device according to claim 1, which is characterized in that the table of the support column (2)
Bread is wrapped with resilient cushion, and the resilient cushion is coated with silencing pad, and the surface of the silencing pad is coated with antistatic japanning.
5. a kind of quantum dot fluorescence detection device according to claim 1, which is characterized in that the master controller (9) with
External power supply is electrically connected, and first cylinder (5), laser emitter (6) and the second cylinder (7) are electrically connected at main control
Device (9), the transmission electron microscope (8) are electrically connected with 200kV high speed voltage source.
6. a kind of quantum dot fluorescence detection device according to claim 1, which is characterized in that the sepectrophotofluorometer
(1302) model Cary Eclipse sepectrophotofluorometer.
7. a kind of quantum dot fluorescence detection method using any one of the claim 1-6 detection device, which is characterized in that institute
State fluorescence detection method are as follows:
S1, by transmission electron microscope (8), scanning obtains the exterior appearance of CdSe/ZnS quantum dot under 200kV high speed voltage, by it
Merging four sides leads in quartz cuvette pond (10), and CdSe/ZnS quantum dot aqueous solution is made；
S2, start the first cylinder (5) by the way that control master controller (9) is positive, extend the length of the first cylinder (5), send out laser
The output end of emitter (6) protrudes into CdSe/ZnS quantum dot aqueous solution, closes the first cylinder (5) by control master controller (9),
Pass through control master controller (9) starting laser emitter (6), ultrasonic 20min, through taking out suction pipe in the second mounting groove (1401)
(1402), the CdSe/ZnS quantum dot aqueous solution after ultrasound is drawn into several drops, is then scattered in the surface of copper mesh (12),
Through the dry 60min of natural cooling；
S3, pass through the positive starting the second cylinder (7) of control master controller (9), the length of elongation the second cylinder (7), so that transmission
When Electronic Speculum (8) is located at close to the upside of copper mesh (12), the second cylinder (7) are closed by control master controller (9), starting transmission electricity
Mirror (8) is measured transmission electron microscope (8) to the CdSe/ZnS quantum dot after abundant drying；
S4, dopamine and water are taken, dopamine and water is made into the standard solution of final concentration of 10 μm of ol/L, through third mounting groove
(1501) colorimetric cylinder (1502) are taken out in, sequentially add the phosphate buffer that 0.5mL concentration is 2.0 × 105 μm of ol/L
(PH6.5-8.0), 0.01mL concentration is the CdSe/ZnS quantum dot solution of 0.08 μm of ol/L, is settled to 5mL with water, shakes up, in
After reacting 20min at room temperature, through taking out sepectrophotofluorometer (1302) in the first mounting groove (1301), fluorescence spectrophotometer light is used
Degree meter (1302) is to a progress fluorescence detection.
8. a kind of quantum dot fluorescence detection method according to claim 7, which is characterized in that the PH of the phosphate buffer
Value is 6.5-8.0, and the pH value of the phosphate buffer takes PHS-3C type pH meter to be measured.
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Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080305047A1 (en) * | 2005-12-19 | 2008-12-11 | Raymo Francisco M | Chemosensors Based on Quantum Dots and Oxazine Compounds |
| CN102012358A (en) * | 2010-10-15 | 2011-04-13 | 济南大学 | Study and application of quantum dot molecular imprinting microsphere quartz fluorescent sensor for detecting trace multicomponent food additives quickly on site |
| CN102288763A (en) * | 2011-07-06 | 2011-12-21 | 清华大学深圳研究生院 | Immunofluorescence method and special kit for detecting ractopamine based on quantum dots |
| CN103411935A (en) * | 2013-07-19 | 2013-11-27 | 中国科学院合肥物质科学研究院 | Method for visualized detection of organophosphorus pesticide residue by doped quantum dot ratio fluorescence technique |
| CN205450006U (en) * | 2016-03-23 | 2016-08-10 | 周口职业技术学院 | A full-automatic routine urine analyzer |
| CN106047342A (en) * | 2016-06-23 | 2016-10-26 | 南京理工大学 | Carbon quantum dot/aurum cluster ratiometric fluorescent probe for detection of cadmium ion and ascorbic acid |
| CN207056620U (en) * | 2017-06-20 | 2018-03-02 | 莫秀林 | A kind of clinical examination multi-function test stand |
| CN107794039A (en) * | 2017-10-27 | 2018-03-13 | 江苏大学 | A kind of preparation and its application for being used to detect the boric acid carbon quantum dot of fluorine ion |
| CN109012785A (en) * | 2017-06-08 | 2018-12-18 | 天津晟俊恒新材料科技有限公司 | New material physical and chemical performance research experiment platform |
-
2018
- 2018-12-26 CN CN201811604821.0A patent/CN109342386A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080305047A1 (en) * | 2005-12-19 | 2008-12-11 | Raymo Francisco M | Chemosensors Based on Quantum Dots and Oxazine Compounds |
| CN102012358A (en) * | 2010-10-15 | 2011-04-13 | 济南大学 | Study and application of quantum dot molecular imprinting microsphere quartz fluorescent sensor for detecting trace multicomponent food additives quickly on site |
| CN102288763A (en) * | 2011-07-06 | 2011-12-21 | 清华大学深圳研究生院 | Immunofluorescence method and special kit for detecting ractopamine based on quantum dots |
| CN103411935A (en) * | 2013-07-19 | 2013-11-27 | 中国科学院合肥物质科学研究院 | Method for visualized detection of organophosphorus pesticide residue by doped quantum dot ratio fluorescence technique |
| CN205450006U (en) * | 2016-03-23 | 2016-08-10 | 周口职业技术学院 | A full-automatic routine urine analyzer |
| CN106047342A (en) * | 2016-06-23 | 2016-10-26 | 南京理工大学 | Carbon quantum dot/aurum cluster ratiometric fluorescent probe for detection of cadmium ion and ascorbic acid |
| CN109012785A (en) * | 2017-06-08 | 2018-12-18 | 天津晟俊恒新材料科技有限公司 | New material physical and chemical performance research experiment platform |
| CN207056620U (en) * | 2017-06-20 | 2018-03-02 | 莫秀林 | A kind of clinical examination multi-function test stand |
| CN107794039A (en) * | 2017-10-27 | 2018-03-13 | 江苏大学 | A kind of preparation and its application for being used to detect the boric acid carbon quantum dot of fluorine ion |
Non-Patent Citations (1)
| Title |
|---|
| 申晨凡等: "基于CdSe/ZnS量子点构建多巴胺荧光检测方法的研究", 《分析测试学报》 * |
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