CN109342381A - A kind of fluorescent dyeing reagent - Google Patents
A kind of fluorescent dyeing reagent Download PDFInfo
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- CN109342381A CN109342381A CN201811396133.XA CN201811396133A CN109342381A CN 109342381 A CN109342381 A CN 109342381A CN 201811396133 A CN201811396133 A CN 201811396133A CN 109342381 A CN109342381 A CN 109342381A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
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Abstract
The invention discloses a kind of fluorescent dyeing reagent, the fluorescent dye in reagent can mark leucocyte, fungi, bacterium, clues cell, cervical exfoliated cell, trichomonad in leukorrhea.Product of the present invention is easy to operate, only needs single stepping that dyeing can be completed；Dyeing time is short, can be observed within one minute；Compared to traditional Microscopical Method For Detection, sensibility and positive rate are higher；It is as a result more intuitive, accurate compared to dry chemical enzyme process, while can preferably solve the case where mixed type infects.Clinical use is more convenient, quick, greatly improves the diagnosis efficiency of vaginitis and cervicitis.
Description
Technical field
The invention belongs to biomedical diagnostic techniques fields, and in particular to a kind of leukorrhea fluorescent dyeing reagent.
Background technique
Vaginitis is current clinical in the common gynecological disease as caused by pathogenic bacteria, different according to the type of pathogen,
Clinic is typically divided into following three classes: bacterial vaginitis, monilial vaginitis, trichomonas vaginitis.Bacterial vaginitis
Patient is mainly shown as that vaginal fluid increases, and has a fish like smell, and especially aggravates after sexual intercourse, can itch with slight vulva convulsion or scorching hot
Sense.Mainly caused by the endogenous mixed infection caused by the reduction of intravaginal lactobacillus, Gardnerella and anaerobic bacteria etc. increase.It reads
Pearl bacterium property vaginitis shows as vulvar erythema, oedema, white membranoid substance.Secretion white stiff is in curdled milk or bean dregs sample.80% 1
90% pathogen is Candida albicans.Common inducement includes gestation, diabetes, widely applies immunosuppressor and broad-spectrum antibiotic.
Trichomonas vagina shows as scorching vagina strength film hyperemia, is dispersed in bleeding spot, and curved grand volume leukorrhea after " the careless sample of grass " uterine neck, is in sallow
Color, yellow-white wash or yellow green purulent secretion, normal show bubble.Main pathogenic bacteria is trichomonas vaginalis infection.
The main purpose that vaginal fluid pathogen checks is that whether there is or not monilial vaginitis, trichomonas vagina for Diagnosis of Female
Scorching and bacterial vaginosis BV.Traditional vaginal fluid detection of pathogens method mainly include direct Microscopical Method For Detection, Glan decoration method,
Isolated culture and Enzyme Assay Method.Traditional bacterial vaginitis, monilial vaginitis, trichomonad are enumerated separately below
Property vaginitis detection method.
Bacterial vaginitis: 1. clues cell inspections are dyed by ordinary optical microscope combination Glan, see clue under mirror
Cell can determine that as bacterial vaginitis.Clues cell is that the vagina squamous cell surface to fall off is covered with many clubs
Bacterium makes cell in mottled, grainy appearance, and cell edges are smudgy to be serrated.Disadvantage: positive rate is low, form not allusion quotation
Type, which is be easy to cause, fails to pinpoint a disease in diagnosis, and reviewer's subjective error is larger.2. occurring when sialidase detection method bacterial vaginosis BV some
Microorganism can produce sialidase, measure the activity of the enzyme in vaginal fluid by chromogenic reaction to diagnose BV indirectly.It lacks
Point: it needs to detect immediately after vaginal fluid materials, otherwise enzyme can lose activity;It is in detection process to need 37 DEG C of incubation processes more,
Operating process shows slightly cumbersome, and zymetology reaction result is not intuitive, be easy to cause false positive.
Colpomycosis: 1. direct Microscopical Method For Detection direct smear microscopies are that taken sample is placed directly on slide, with dye
Color adds 10%KOH solution, checks under light microscopic.But the contrast between fungi and histocyte is little, and it is very tired to identify fungi
It is difficult, it is also necessary to further culture identification.In addition, a possibility that directly microscopy feminine gender can not exclude fungal infection.2. separation training
The method of supporting cultivates fungi from clinical samples, it is therefore intended that fungi is separated from clinical samples, it is true to determine whether
Bacterium infection, especially when direct microscopy is negative.Disadvantage: although cultivation testing result accuracy is high, since fungi is raw
Long slow, culture often needs even several weeks a couple of days just to have as a result, can often be delayed conditions of patients.
Trichomonas vaginitis: vaginal fluid swab specimen is put into lml physiological saline test tube by 1. direct Microscopical Method For Detections, is mixed
Absorption one, which is dripped, after even searches trichomonad under glass slide, covered, microscope.Disadvantage: needing to detect immediately after specimen sampling,
Otherwise overlong time trichomonad is easy dead and is not easy to observe;There are certain technical requirements to reviewer, has biggish subjectivity accidentally
Difference.2. antigen detection method detects in vaginal fluid with the presence or absence of trichomonas vaginalis antigen to determine whether there is trichomonas vaginalis.
Trichomonas vaginalis antigen detecting agent, principle are immunochromatographic method, and sensibility is high, but result is not intuitive, and there are certain vacations
It is positive.
Vagina pathogen joint-detection: referred to by a variety of biochemical or zymetologys of measurement vaginal fluid and cervical exfoliated cell
Mark, including pH value, catalase, leucocyte lipase, the general enzyme of sialic acid, the general enzyme of second phthalein Glucosamine pass through these zymetologys
Index comprehensive judges the microorganism infection situation of patient.The disadvantage is that the judgement value of some indexs needs further to be proved, have
Index does not have specific scientific basis, and clinical actual use result is also undesirable.Particularly with sentencing for mixed type infection conditions
It is disconnected, there is very big error.
Summary of the invention
The present invention, can be same in view of the shortcomings of the prior art, develop a kind of leukorrhea rapid fluorescence dyeing liquor of one-pack type
Leucocyte, fungi, bacterium, clues cell, cervical exfoliated cell, trichomonad are detected on one glass slide simultaneously.Leukorrhea sample passes through
After this reagent dyeing, by single band fluorescence microscope carry out observation you can get it check conclusion.Product of the present invention is easy to operate,
Only need single stepping that dyeing can be completed；Dyeing time is short, can be observed within one minute;Compared to traditional microscopy
Method, sensibility and positive rate are higher；It is as a result more intuitive, accurate compared to dry chemical enzyme process, while can preferably solve mixed type
The case where infection.Clinical use is more convenient, quick, greatly improves the diagnosis efficiency of vaginitis and cervicitis.
Specific technical solution of the present invention is as follows:
A kind of fluorescent dyeing reagent, it is characterised in that include A group, B group, C group substance.
A group substance of the present invention includes one of following substance or a variety of: calcium fluorescent whitening agent, calcium fluorescent whitening agent
Sodium salt, calcium fluorescent whitening agent sylvite, calcium fluorescent whitening agent ammonium salt, using calcium fluorescent whitening agent as the derivative of parent nucleus, with calcium fluorescence
Brightening agent be parent nucleus derivative formed salt, anti-chitin antibody and its fluorescent material marker, anti-mannosan antibody and
Its fluorescent marker, propidium iodide and its derivative, ethidium bromide and its derivative, calcein (Calcein) and its derivative
Object, JC-1(5,5 ', 6,6 '-Tetrachloro-1,1 ', 3,3 '-tetraethylbenzimidazolylcarbocyanine
) and its derivative, JC-10(5,6-Dichloro-1,1 ', 3,3 '-tetraethyl-imidacarbocyanine iodide
Iodide) and its derivative, FCCP(Carbonyl cyanide 4- (trifluoromethoxy) phenylhydrazone)
And its derivative, staurosporine and derivative, phalloidine and its fluorescent marker, ethidium bromide list nitrine bromine and its derivative
Object, Ethidium Homodimer-1 (EthD-1) and its derivative, 7-aminoactinomycin D, Chromomycin A3 and
Its derivative, Sodium-binding Benzofuran Isophthalate and its derivative, 2-NBDG(2-Deoxy-2-
[(7-nitro-2,1,3-benzoxadiazol-4-yl) amino]-D-glucose) and derivative, the trip of D- firefly luciferin
From acid and its derivative, Carboxy-PTIO(2- (4-Carboxyphenyl) -4,4,5,5-
) and its anti-oxidant albumen Prx I& of derivative, Conoidin A tetramethylimidazoline-1-oxyl-3-oxide
II inhibitor and its derivative, DRAQ5 and its derivative, Ethidium Homodimer-III and its derivative, Hydroxystilbamidine
And its derivative, CytoMBrite Red Cytoplasmic Membrane Dye and its derivative, CytoMBrite
Orange Cytoplasmic Membrane Dye and its derivative, CytoMBrite Green Cytoplasmic
Membrane Dye and its derivative, CytoMBrite NIR680 and its derivative.More preferable DRAQ5.Preferred concentration is
0.1%-5%(W/V)。
B group substance of the present invention includes one of following substance or a variety of: (1,5-IAEDANS, 5- (((2-
Iodoacetyl) amino) ethyl) amino) Naphthalene-1-Sulfonic Acid) salt and derivative, 8- aniline-
The salt and derivative, 4- of 1-naphthalene sulfonic aicd (8-Anilino-1-naphthalenesulfonic acid)
The salt and derivative of Methylumbelliferone, the salt of carboxy-2,7-dichlorofluorescein and derivative,
The salt and derivative of Carboxyfluorescein, the salt of Carboxytetramethylrhodamine and derivative,
The salt and derivative of Hydroxy Tryptamine, the salt of carboxy-X-rhodamine and derivative, 6-
Salt and derivative, 6-JOE (6- carboxyl -4 ', 5 '-two chloro- 2 ', the 7 '-dimethoxyfluorescein ambers of Carboxyrhodamine
Imide ester) salt and derivative, the salt of 7-Amino-4-methylcoumarin and derivative, 7-Aminoactinomycin
Salt and derivative, the salt of 7-Hydroxy-4-methylcoumarin and derivative, 9-Amino-6- of D (7-AAD)
The salt and derivative of chloro-2-methoxyacridine, the salt of Acid Fuchsin and derivative, 9-Amino-6-
The salt and derivative of chloro-2-methoxyacridine, the salt of Acridine Orange and derivative, Acridine Red
Salt and derivative, the salt of Acridine Yellow and derivative, the salt of Acriflavin and derivative, Acriflavin
The salt and derivative, AFPs-of the salt and derivative of Feulgen SITSA, Aequorin (Photoprotein)
The salt of AutoFluorescent Protein-(Quantum Biotechnologies) see sgGFP, sgBFP and spread out
Biology, the salt of Alexa Fluor 350 and derivative, the salt of Alexa Fluor 430 and derivative, Alexa Fluor 488
Salt and derivative, the salt of Alexa Fluor 532 and derivative, the salt of Alexa Fluor 546 and derivative, Alexa
The salt and derivative of Fluor 568, the salt of Alexa Fluor 594 and derivative, the salt of Alexa Fluor 633 and derivative
Object, the salt of Alexa Fluor 647 and derivative, the salt of Alexa Fluor 660 and derivative, Alexa Fluor 680
Salt and derivative, the salt of Alizarin Complexon and derivative, the salt of Alizarin Red and derivative,
The salt and derivative of Allophycocyanin (APC), the salt of AMC (7- amino -4- methylcoumarin) and derivative,
The salt and derivative of Aminoactinomycin D, the salt of Aminocoumarin and derivative, Anilin Blue salt and spread out
The salt and derivative, APC-Cy7 of biology, the salt of Anthrocyl stearate and derivative, APC (Allophycocyanin)
Salt and derivative, the salt of Astrazon Brilliant Red 4G and derivative, Astrazon Orange R salt and spread out
Biology, the salt of Astrazon Red 6B and derivative, the salt of 7 GLL of Astrazon Yellow and derivative, Atabrine
Salt and derivative, the salt of ATTO-TAG CBQCA and derivative, the salt of ATTO-TAG FQ and derivative, Auramine salt and
Derivative, the salt of Aurophosphine G and derivative, the salt of Aurophosphine and derivative, BAO 9
(Bisaminophenyloxadiazole) salt and derivative, BCECF (low of salt and derivative, BCECF (high pH)
PH the salt and derivative, the salt of Beta Lactamase and derivative, BFP of salt and derivative, Berberine Sulphate)
The salt and derivative, BFP/ of the salt and derivative of blue shifted GFP (Y66H), Blue Fluorescent Protein
The salt and derivative of GFP FRET, the salt of Bimane and derivative, the salt of Bisbenzamide and derivative, Bisbenzimide
The salt and derivative, the salt of Blancophor SV and derivative of salt and derivative, Blancophor FFG (Hoechst),
The salt and derivative of BOBO-1, the salt of BOBO-3 and derivative, the salt of Bodipy 492/515 and derivative, Bodipy 493/
503 salt and derivative, the salt of Bodipy 500/510 and derivative, the salt of Bodipy 505/515 and derivative, Bodipy
530/550 salt and derivative, the salt of Bodipy 542/563 and derivative, the salt of Bodipy 558/568 and derivative,
The salt and derivative of Bodipy 564/570, the salt of Bodipy 576/589 and derivative, propidium iodide and its derivative,
The salt of the salt and derivative of Bodipy 581/591, the salt of Bodipy 630/650-X and derivative, Bodipy 650/665-X
And derivative, the salt of Bodipy 665/676 and derivative, the salt of Bodipy Fl and derivative, Bodipy FL ATP salt and
Derivative, the salt of Bodipy Fl-Ceramide and derivative, the salt of Bodipy R6G SE and derivative, Bodipy TMR
Salt and derivative, the salt of Bodipy TMR-X conjugate and derivative, the salt of Bodipy TMR-X, SE and derivative,
The salt and derivative of Bodipy TR, the salt of Bodipy TR ATP and derivative, the salt of Bodipy TR-X SE and derivative,
The salt and derivative of the salt and derivative of BO-PRO-1, the salt of BO-PRO-3 and derivative, Brilliant Sulphoflavin FF
Object, the salt of BTC-Ratio Dye Ca2+ and derivative, the salt of BTC-5N-atio Dye, Zn2+ and derivative,
The salt and derivative of Calcein, the salt of Calcein Blue and derivative, the salt of Calcium Crimson and derivative,
The salt and derivative, Calcium of the salt and derivative of Calcium Green, Calcium Green-1 Ca2+ Dye
The salt and derivative of Green-2 Ca2+, the salt of Calcium Green-5N Ca2+ and derivative, Calcium Green-
The salt and derivative of C18 Ca2+, the salt of Calcium Orange and derivative, the salt of Cascade Blue and derivative,
The salt and derivative of Cascade Yellow, the salt of Catecholamine and derivative, CCF2 (GeneBlazer) salt and
Derivative, CFDA(carboxyfluorescein diacetate) salt and derivative, CFP(Cyan Fluorescent Protein) salt
And derivative, the salt of CFP/YFP FRET and derivative, the salt of Chlorophyll and derivative, Chromomycin A salt and
The salt and derivative, CMFDA(5-Chloromethylfluorescein of derivative, CL-NERF (Ratio Dye, pH)
The salt and derivative of salt and derivative, Coelenterazine Ca2+ Dye bioluminescence Diacetate),
The salt and derivative, the salt of Coelenterazine f and derivative of Coelenterazine cp (Ca2+ Dye),
The salt and derivative of Coelenterazine fcp, the salt of Coelenterazine h and derivative, Coelenterazine
The salt and derivative of hcp, the salt of Coelenterazine ip and derivative, the salt of Coelenterazine n and derivative,
The salt and derivative of Coelenterazine O, the salt of Coumarin Phalloidin and derivative, C-phycocyanine
Salt and derivative, the salt of CPM Methylcoumarin and derivative, the salt of Cy2 and derivative, the salt of Cy3.1 8 and derivative
Object, the salt of Cy3.5 and derivative, the salt of Cy3 and derivative, the salt of Cy5.1 8 and derivative, the salt of Cy5.5 and derivative,
The salt and derivative of Cy5, the salt of Cy7 and derivative, the salt of Cyan GFP and derivative, cyclic AMP Fluorosensor
(FiCRhR) salt and derivative, the salt of CyQuant Cell Proliferation Assay and the salt of derivative, Dabcyl
And the salt and derivative of derivative, Dansyl, the salt of Dansyl Amine and derivative, Dansyl Cadaverine salt and
Derivative, the salt of Dansyl Chloride and derivative, the salt of Dansyl DHPE and derivative, Dansyl fluoride
Salt and derivative, the salt of DAPI (4', 6-Diamidino-2-Phenylindole, Dihydrochloride) and derivative,
The salt and derivative of Dapoxyl, the salt of Dapoxyl 2 and derivative, the salt of Dapoxyl 3 and derivative, DCFH
Salt and derivative, the DDAO [7-hydroxy-9H- (1,3- of (Dichlorodihydrofluorescein Diacetate)
Dichloro-9,9-dimethylacridin-2-one salt and derivative)], DHR (Dihydorhodamine 123)
The salt and derivative, DiA of salt and derivative, the salt of Di-4-ANEPPS and derivative, Di-8-ANEPPS (non-ratio)
The salt and derivative of (4-Di-16-ASP), the salt of Dichlorodihydrofluorescein Diacetate (DCFH) and spread out
The salt and derivative, DIDS(4 of biology, the salt of DiD-Lipophilic Tracer and derivative, DiD (DiIC18 (5)),
4'- diisothiocyanic acid base -2,2'- stilbenesulphonic acid) salt and derivative, the salt of DiI (DiIC18 (3)) and derivative,
The salt and derivative of Dinitrophenol, the salt of DiO (DiOC18 (3)) and derivative, DiR (DiIC18 (7)) salt and spread out
Biology, DNP(2,4-dinitropheno2,4- dinitrophenol) salt and derivative, the salt of Dopamine and derivative,
Salt and derivative, the DTAF((4,6-Dichlorotriazinyl of DsRed) aminofluorescein) salt and derivative,
The enhanced blue fluorescent protein of EBFP() salt and the enhanced cyan fluorescent protein of derivative, ECFP() salt and derivative,
EGFP(enhanced green fluorescence protein) salt and derivative, ELF 97(enzyme mark fluorescence 97) salt and derivative, Eosin salt
And the salt and derivative of derivative, Erythrosin, the salt of Erythrosin ITC and derivative, Ethidium Bromide
Salt and derivative, the salt of Ethidium homodimer -1 (EthD-1) and derivative, the salt of Euchrysin and derivative,
The salt and the enhanced yellow fluorescence of derivative, EYFP(of the salt and derivative of EukoLight, Europium (III) chloride
Albumen) salt and derivative, the salt of Fast Blue and derivative, FDA(fluorescein diacetate) salt and derivative,
The salt and derivative of Feulgen (Pararosaniline), FIF (Formaldehyd Induced Fluorescence)
Salt and derivative, the salt of Flazo Orange and derivative, Fluo-3(calcium ion fluorescent dye Fluo-3) salt and derivative,
Fluo-4(calcium ion fluorescent dye Fluo-4) salt and derivative, the salt of Fluorescein (FITC) and derivative,
The salt and derivative of Fluorescein Diacetate, the salt of Fluoro-Emerald and derivative, Fluoro-Gold
(Hydroxystilbamidine) salt and derivative, Genacryl Brilliant of salt and derivative, Fluor-Ruby
The salt and derivative of Red B, the salt of Genacryl Brilliant Yellow 10GF and derivative, Genacryl Pink 3G
Salt and derivative, the salt of Genacryl Yellow 5GF and derivative, the salt of GeneBlazer (CCF2) and derivative,
The salt and derivative of Gloxalic Acid, the salt of Granular Blue and derivative, Haematoporphyrin salt and spread out
The salt and derivative of biology, the salt of Hoechst 33258 and derivative, Hoechst 33342.More preferable Fluo-4.It is preferred that dense
Degree is 0.1%-5% (W/V).
C group substance of the present invention includes one of following substance or a variety of: water, glycerol, DMSO(dimethyl sulfoxide),
KOH(potassium hydroxide), NaOH(sodium hydroxide).
Specific embodiment
Illustrate specific steps of the invention by the following examples, but is not limited by the example.
Term as used in the present invention generally there are those of ordinary skill in the art usually to manage unless otherwise indicated
The meaning of solution.
The present invention is described in further detail combined with specific embodiments below and referring to data.It should be understood that these embodiments are
In order to demonstrate the invention, it rather than limits the scope of the invention in any way.
In the examples below, the various processes and method being not described in detail are conventional methods as known in the art.
The preparation of 1 fluorescent dyeing reagent of embodiment
DRAQ5, Fluo-4 are add to deionized water, the concentration 0.2% (W/V), Fluo-4 for sufficiently making DRAQ5 are dissolved
Concentration is 0.1% (W/V).
The technical concepts and features of above embodiment only to illustrate the invention, its object is to allow be familiar with technique
People cans understand the content of the present invention and implement it accordingly, and it is not intended to limit the scope of the present invention.It is all according to the present invention
The equivalent transformation or modification that Spirit Essence is done, should be covered by the protection scope of the present invention.
Claims (5)
1. a kind of fluorescent dyeing reagent, which is characterized in that the fluorescent dye in reagent can mark leucocyte in leukorrhea, fungi,
Bacterium, clues cell, cervical exfoliated cell, trichomonad.
2. fluorescent dyeing reagent as described in claim 1, it is characterised in that include A group, B group, C group substance.
3. fluorescent dyeing reagent as claimed in claim 2, it is characterised in that the A group substance includes one in following substance
Kind or it is a variety of: calcium fluorescent whitening agent, calcium fluorescent whitening agent sodium salt, calcium fluorescent whitening agent sylvite, calcium fluorescent whitening agent ammonium salt, with calcium
Fluorescent whitening agent be parent nucleus derivative, using calcium fluorescent whitening agent as parent nucleus derivative formed salt, anti-chitin antibody and
Its fluorescent material marker, anti-mannosan antibody and its fluorescent marker, propidium iodide and its derivative, ethidium bromide and its
Derivative, calcein (Calcein) and its derivative, JC-1(5,5 ', 6,6 '-Tetrachloro-1,1 ', 3,3 '-tetr
Aethylbenzimidazolylcarbocyanine iodide) and its derivative, JC-10(5,6-Dichloro-1,1 ',
3,3 '-tetraethyl-imidacarbocyanine iodide) and its derivative, FCCP(Carbonyl cyanide 4-
(trifluoromethoxy) phenylhydrazone) and its derivative, staurosporine and derivative, phalloidine and its glimmering
Signal object, ethidium bromide list nitrine bromine and its derivative, Ethidium Homodimer-1 (EthD-1) and its derivative,
7-aminoactinomycin D, Chromomycin A3 and its derivative, Sodium-binding Benzofuran
Isophthalate and its derivative, 2-NBDG(2-Deoxy-2- [(7-nitro-2,1,3-benzoxadiazol-4-yl)
Amino]-D-glucose) and derivative, D- firefly luciferin free acid and its derivative, Carboxy-PTIO(2- (4-
Carboxyphenyl) -4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide) and its derivative,
The anti-oxidant albumen Prx I&II inhibitor of Conoidin A and its derivative, DRAQ5 and its derivative, Ethidium
Homodimer-III and its derivative, Hydroxystilbamidine and its derivative, CytoMBrite Red Cytoplasmic
Membrane Dye and its derivative, CytoMBrite Orange Cytoplasmic Membrane Dye and its derivative
Object, CytoMBrite Green Cytoplasmic Membrane Dye and its derivative, CytoMBrite NIR680
And its derivative.
4. fluorescent dyeing reagent as described in claim 1, it is characterised in that the B group substance includes one in following substance
Kind is a variety of: (1,5-IAEDANS, 5- (((2-Iodoacetyl) amino) ethyl) amino) Naphthalene-1-
Sulfonic Acid) salt and derivative, 8- aniline -1-naphthalene sulfonic aicd (8-Anilino-1-naphthalenesulfonic
Acid the salt and derivative, carboxy-2,7- of salt and derivative, 4-Methylumbelliferone)
The salt and derivative of dichlorofluorescein, the salt of Carboxyfluorescein and derivative,
The salt and derivative of Carboxytetramethylrhodamine, the salt of Hydroxy Tryptamine and derivative,
The salt and derivative of carboxy-X-rhodamine, the salt of 6-Carboxyrhodamine and derivative, 6-JOE (6- carboxyl-
4 ', 5 '-two chloro- 2 ', 7 '-dimethoxyfluorescein succinimide esters) salt and derivative, 7-Amino-4-
The salt and derivative, 7- of the salt and derivative of methylcoumarin, 7-Aminoactinomycin D (7-AAD)
The salt and derivative of Hydroxy-4-methylcoumarin, the salt of 9-Amino-6-chloro-2-methoxyacridine and
Derivative, the salt of Acid Fuchsin and derivative, the salt of 9-Amino-6-chloro-2-methoxyacridine and derivative
Object, the salt of Acridine Orange and derivative, the salt of Acridine Red and derivative, Acridine Yellow salt and
The salt and derivative, Aequorin of derivative, the salt of Acriflavin and derivative, Acriflavin Feulgen SITSA
(Photoprotein) salt and derivative, AFPs-AutoFluorescent Protein-(Quantum
The salt and derivative of Biotechnologies) see sgGFP, sgBFP, the salt of Alexa Fluor 350 and derivative,
The salt and derivative of Alexa Fluor 430, the salt of Alexa Fluor 488 and derivative, Alexa Fluor 532 salt and
Derivative, the salt of Alexa Fluor 546 and derivative, the salt of Alexa Fluor 568 and derivative, Alexa Fluor
594 salt and derivative, the salt of Alexa Fluor 633 and derivative, the salt of Alexa Fluor 647 and derivative, Alexa
The salt and derivative of Fluor 660, the salt of Alexa Fluor 680 and derivative, Alizarin Complexon salt and spread out
Biology, the salt of Alizarin Red and derivative, the salt of Allophycocyanin (APC) and derivative, AMC (7- amino -4-
Methylcoumarin) salt and derivative, the salt of Aminoactinomycin D and derivative, Aminocoumarin salt and spread out
Biology, the salt of Anilin Blue and derivative, the salt of Anthrocyl stearate and derivative, APC
(Allophycocyanin) salt and derivative of salt and derivative, APC-Cy7, Astrazon Brilliant Red 4G
The salt and derivative, Astrazon of salt and derivative, the salt of Astrazon Orange R and derivative, Astrazon Red 6B
The salt and derivative of 7 GLL of Yellow, the salt of Atabrine and derivative, the salt of ATTO-TAG CBQCA and derivative,
The salt and derivative of ATTO-TAG FQ, the salt of Auramine and derivative, the salt of Aurophosphine G and derivative,
The salt and derivative, BCECF of the salt and derivative of Aurophosphine, BAO 9 (Bisaminophenyloxadiazole)
The salt and derivative, the salt of Berberine Sulphate and derivative of the salt and derivative of (high pH), BCECF (low pH)
The salt and derivative, Blue of object, the salt of Beta Lactamase and derivative, BFP blue shifted GFP (Y66H)
The salt and derivative of Fluorescent Protein, the salt of BFP/GFP FRET and derivative, the salt of Bimane and derivative,
The salt and derivative of Bisbenzamide, the salt of Bisbenzimide (Hoechst) and derivative, Blancophor FFG
Salt and derivative, the salt of Blancophor SV and derivative, the salt of BOBO-1 and derivative, the salt of BOBO-3 and derivative,
The salt and derivative of Bodipy 492/515, the salt of Bodipy 493/503 and derivative, Bodipy 500/510 salt and spread out
The salt of biology, the salt of Bodipy 505/515 and derivative, the salt of Bodipy 530/550 and derivative, Bodipy 542/563
And derivative, the salt of Bodipy 558/568 and derivative, the salt of Bodipy 564/570 and derivative, Bodipy 576/589
Salt and derivative, propidium iodide and its derivative, the salt of Bodipy 581/591 and derivative, Bodipy 630/650-X
Salt and derivative, the salt of Bodipy 650/665-X and derivative, the salt of Bodipy 665/676 and derivative, Bodipy Fl
Salt and derivative, the salt of Bodipy FL ATP and derivative, the salt of Bodipy Fl-Ceramide and derivative, Bodipy
The salt and derivative of R6G SE, the salt of Bodipy TMR and derivative, the salt of Bodipy TMR-X conjugate and derivative,
The salt and derivative of Bodipy TMR-X, SE, the salt of Bodipy TR and derivative, the salt of Bodipy TR ATP and derivative,
The salt and derivative of Bodipy TR-X SE, the salt of BO-PRO-1 and derivative, the salt of BO-PRO-3 and derivative,
The salt and derivative, BTC-5N of the salt and derivative of Brilliant Sulphoflavin FF, BTC-Ratio Dye Ca2+
The salt and derivative of-atio Dye, Zn2+, the salt of Calcein and derivative, the salt of Calcein Blue and derivative,
The salt and derivative of Calcium Crimson, the salt of Calcium Green and derivative, Calcium Green-1 Ca2+
The salt of the salt and derivative of Dye, the salt of Calcium Green-2 Ca2+ and derivative, Calcium Green-5N Ca2+
And derivative, the salt of Calcium Green-C18 Ca2+ and derivative, the salt of Calcium Orange and derivative,
The salt and derivative of Cascade Blue, the salt of Cascade Yellow and derivative, the salt of Catecholamine and derivative
Object, the salt of CCF2 (GeneBlazer) and derivative, CFDA(carboxyfluorescein diacetate) salt and derivative, CFP
The salt and derivative, the salt of CFP/YFP FRET and derivative of (Cyan Fluorescent Protein), Chlorophyll
Salt and derivative, the salt of Chromomycin A and derivative, the salt of CL-NERF (Ratio Dye, pH) and derivative,
CMFDA(5-Chloromethylfluorescein Diacetate) salt and derivative, Coelenterazine Ca2+
The salt and derivative of Dye bioluminescence, the salt of Coelenterazine cp (Ca2+ Dye) and derivative,
The salt and derivative of Coelenterazine f, the salt of Coelenterazine fcp and derivative, Coelenterazine h
Salt and derivative, the salt of Coelenterazine hcp and derivative, the salt of Coelenterazine ip and derivative,
The salt and derivative of Coelenterazine n, the salt of Coelenterazine O and derivative, Coumarin Phalloidin
Salt and derivative, the salt of C-phycocyanine and derivative, the salt of CPM Methylcoumarin and derivative, Cy2
The salt of salt and derivative, the salt of Cy3.1 8 and derivative, the salt of Cy3.5 and derivative, the salt of Cy3 and derivative, Cy5.1 8
And the salt and derivative, the salt of Cy7 and derivative, the salt of Cyan GFP and derivative of the salt and derivative of derivative, Cy5.5, Cy5
Object, the salt of cyclic AMP Fluorosensor (FiCRhR) and derivative, CyQuant Cell Proliferation
The salt and derivative of Assay, the salt of Dabcyl and derivative, the salt of Dansyl and derivative, Dansyl Amine salt and spread out
Biology, the salt of Dansyl Cadaverine and derivative, the salt of Dansyl Chloride and derivative, Dansyl DHPE
Salt and derivative, the salt of Dansyl fluoride and derivative, DAPI (4', 6-Diamidino-2-Phenylindole,
The salt and derivative, the salt of Dapoxyl 2 and derivative of salt and derivative, Dapoxyl Dihydrochloride),
The salt and derivative of the salt and derivative of Dapoxyl 3, DCFH (Dichlorodihydrofluorescein Diacetate)
Object, the salt of DDAO [7-hydroxy-9H- (1,3-dichloro-9,9-dimethylacridin-2-one)] and derivative,
Salt and derivative, the salt of Di-4-ANEPPS and derivative, Di-8-ANEPPS of DHR (Dihydorhodamine 123)
The salt and derivative of salt and derivative, DiA (4-Di-16-ASP) (non-ratio),
Salt and derivative, the DiD-Lipophilic of Dichlorodihydrofluorescein Diacetate (DCFH)
The salt and derivative of Tracer, the salt of DiD (DiIC18 (5)) and derivative, DIDS(4,4'- diisothiocyanic acid base -2,2'- two
Styrene sulfonic acid) salt and derivative, DiI (DiIC18 (3)) salt and derivative, the salt of Dinitrophenol and derivative
The salt and derivative, DNP(2,4- of object, the salt of DiO (DiOC18 (3)) and derivative, DiR (DiIC18 (7))
Dinitropheno2,4- dinitrophenol) salt and derivative, the salt of Dopamine and derivative, the salt of DsRed and derivative
Object, DTAF((4,6-Dichlorotriazinyl) aminofluorescein) salt and the enhanced blue of derivative, EBFP(
Fluorescin) salt and the enhanced cyan fluorescent protein of derivative, ECFP() salt and derivative, EGFP(enhanced green
Fluorescin) salt and derivative, ELF 97(enzyme mark fluorescence 97) salt and derivative, the salt of Eosin and derivative,
The salt and derivative of Erythrosin, the salt of Erythrosin ITC and derivative, the salt of Ethidium Bromide and derivative
Object, the salt of Ethidium homodimer -1 (EthD-1) and derivative, the salt of Euchrysin and derivative, EukoLight
Salt and derivative, Europium (III) chloride salt and the enhanced yellow fluorescence protein of derivative, EYFP() salt
And derivative, the salt of Fast Blue and derivative, FDA(fluorescein diacetate) salt and derivative, Feulgen
(Pararosaniline) salt and derivative of salt and derivative, FIF (Formaldehyd Induced Fluorescence)
Object, the salt of Flazo Orange and derivative, Fluo-3(calcium ion fluorescent dye Fluo-3) salt and derivative, Fluo-4
The salt and derivative, Fluorescein of the salt and derivative of (calcium ion fluorescent dye Fluo-4), Fluorescein (FITC)
The salt and derivative of Diacetate, the salt of Fluoro-Emerald and derivative, Fluoro-Gold
(Hydroxystilbamidine) salt and derivative, Genacryl Brilliant of salt and derivative, Fluor-Ruby
The salt and derivative of Red B, the salt of Genacryl Brilliant Yellow 10GF and derivative, Genacryl Pink 3G
Salt and derivative, the salt of Genacryl Yellow 5GF and derivative, the salt of GeneBlazer (CCF2) and derivative,
The salt and derivative of Gloxalic Acid, the salt of Granular Blue and derivative, Haematoporphyrin salt and spread out
The salt and derivative of biology, the salt of Hoechst 33258 and derivative, Hoechst 33342.
5. fluorescent dyeing reagent as claimed in claim 2, it is characterised in that the C group substance includes one of following substance
It is or a variety of: water, glycerol, DMSO(dimethyl sulfoxide), KOH(potassium hydroxide), NaOH(sodium hydroxide).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811396133.XA CN109342381A (en) | 2018-11-22 | 2018-11-22 | A kind of fluorescent dyeing reagent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811396133.XA CN109342381A (en) | 2018-11-22 | 2018-11-22 | A kind of fluorescent dyeing reagent |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109342381A true CN109342381A (en) | 2019-02-15 |
Family
ID=65316827
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811396133.XA Withdrawn CN109342381A (en) | 2018-11-22 | 2018-11-22 | A kind of fluorescent dyeing reagent |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109342381A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110055300A (en) * | 2019-04-18 | 2019-07-26 | 北京大学 | A kind of fungal infection detection kit and its application |
| RU2725291C1 (en) * | 2019-05-27 | 2020-06-30 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Тихоокеанский государственный медицинский университет" Министерства здравоохранения Российской Федерации | Method of donozological non-invasive diagnostics of health of workers |
| CN111781177A (en) * | 2020-06-29 | 2020-10-16 | 安徽德莱康生物医疗科技有限公司 | Detection reagent and preparation method thereof |
| CN112924259A (en) * | 2021-02-01 | 2021-06-08 | 上海师范大学 | Observation method for specifically identifying different parts of pollen, corresponding dye and application |
| CN116256212A (en) * | 2023-05-15 | 2023-06-13 | 广州盛安医学检验有限公司 | Gynecological fluorescence staining solution and preparation method and application thereof |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110055300A (en) * | 2019-04-18 | 2019-07-26 | 北京大学 | A kind of fungal infection detection kit and its application |
| RU2725291C1 (en) * | 2019-05-27 | 2020-06-30 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Тихоокеанский государственный медицинский университет" Министерства здравоохранения Российской Федерации | Method of donozological non-invasive diagnostics of health of workers |
| CN111781177A (en) * | 2020-06-29 | 2020-10-16 | 安徽德莱康生物医疗科技有限公司 | Detection reagent and preparation method thereof |
| CN112924259A (en) * | 2021-02-01 | 2021-06-08 | 上海师范大学 | Observation method for specifically identifying different parts of pollen, corresponding dye and application |
| CN116256212A (en) * | 2023-05-15 | 2023-06-13 | 广州盛安医学检验有限公司 | Gynecological fluorescence staining solution and preparation method and application thereof |
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Application publication date: 20190215 |