CN109342330A - A kind of optical fiber biosensor and its detection method - Google Patents
A kind of optical fiber biosensor and its detection method Download PDFInfo
- Publication number
- CN109342330A CN109342330A CN201811258174.2A CN201811258174A CN109342330A CN 109342330 A CN109342330 A CN 109342330A CN 201811258174 A CN201811258174 A CN 201811258174A CN 109342330 A CN109342330 A CN 109342330A
- Authority
- CN
- China
- Prior art keywords
- fiber
- optical fiber
- biosensor
- coreless
- antibody
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/255—Details, e.g. use of specially adapted sources, lighting or optical systems
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
Abstract
A kind of optical fiber biosensor and its detection method, the optical fiber biosensor include input transmission fiber, coreless fiber and output transmission fiber；The input transmission fiber, the coreless fiber and the output transmission fiber are sequentially connected, and the input transmission fiber is connected with wideband light source, and the output transmission fiber is connected with spectroanalysis instrument；The input transmission fiber and the output transmission fiber are single mode optical fiber, and the coreless fiber is tapered fiber.Optical fiber biosensor provided by the invention, biologic specificity to be measured first, which is modified, in its sensor surface identifies molecule, the sensor of modified is immersed into sample to be tested pond later, the measurement of sample to be tested concentration can be realized by detecting interference spectral changes, and the sensor of modified immerses behind sample to be tested pond in the microspheres solution for immersing modified again can be further improved detection sensitivity.Optical fiber biosensor provided by the invention have the advantages that structure it is simple, quickly, high sensitivity.
Description
Technical field
The present invention relates to technical field of optical fiber sensing more particularly to a kind of optical fiber biosensor and its detection methods.
Background technique
In recent years, the research of fiber-optic biosensor medical diagnosis on disease, environmental monitoring, in terms of have increasingly
Important research significance and application value.In order to widen the approach of bio-sensing detection, new sensor mechanism, development of new are explored
Senser element become extremely urgent.With the introducing of new information functional material-optical fiber, modern biotechnology sensing technology has again
New approach.Optical fiber has light-weight, small in size, high sensitivity, fast response time, electromagnetism interference, safety good, biological simultaneous
The features such as capacitive is strong becomes and develops one of most rapid sensing technology in the latest 20 years.
Realization is highly sensitive, label-free, high specific in the detection of body biomedicine based on Fibre Optical Sensor, and develops
More multifunction, integrated optical fibre bio medical sensor part, oneself becomes research hotspot and the development side in bio-sensing field
To.Therefore, need to research and develop a kind of optical fiber biosensor and its detection method.
Summary of the invention
In order to solve the above technical problems, first aspect present invention provides a kind of optical fiber biosensor, the optical fiber is raw
Object sensor includes input transmission fiber, coreless fiber and output transmission fiber；The input transmission fiber, the coreless fiber
Be sequentially connected with the output transmission fiber, the input transmission fiber is connected with wideband light source, the output transmission fiber and
Spectroanalysis instrument connection；The input transmission fiber and the output transmission fiber are single mode optical fiber, and the coreless fiber is to draw
Bore optical fiber.
Wherein, the length of the coreless fiber is 5~30mm, and the lumbar vertebrae length of the coreless fiber is 5~15mm, described
The lumbar vertebrae diameter of coreless fiber is 1~10 μm.
Preferably,
The length of the coreless fiber is 10mm, 15mm, 20mm, 25mm；
The lumbar vertebrae length of the coreless fiber is 6mm, 7mm, 8mm, 9mm, 10mm, 11mm, 12mm, 13mm, 14mm；The centreless
The lumbar vertebrae diameter of optical fiber is 2 μm, 3 μm, 4 μm, 5 μm, 6 μm, 7 μm, 8 μm, 9 μm.
Second aspect of the present invention provides a kind of optical fiber biosensor, and the optical fiber biosensor includes input transmission
Optical fiber, coreless fiber and output transmission fiber；The input transmission fiber, the coreless fiber and the output transmission fiber according to
Secondary connection, the input transmission fiber are connected with wideband light source, and the output transmission fiber is connected with spectroanalysis instrument；It is described defeated
Enter transmission fiber and the output transmission fiber is single mode optical fiber, the coreless fiber is tapered fiber；The optical fibre bio passes
Sensor further includes the microballoon for enhancing transducer sensitivity.
Wherein, the length of the coreless fiber is 5~30mm, and the lumbar vertebrae length of the coreless fiber is 5~15mm, described
The lumbar vertebrae diameter of coreless fiber is 1~10 μm.
Preferably,
The length of the coreless fiber is 10mm, 15mm, 20mm, 25mm；
The lumbar vertebrae length of the coreless fiber is 6mm, 7mm, 8mm, 9mm, 10mm, 11mm, 12mm, 13mm, 14mm；The centreless
The lumbar vertebrae diameter of optical fiber is 2 μm, 3 μm, 4 μm, 5 μm, 6 μm, 7 μm, 8 μm, 9 μm.
Wherein, the microballoon is magnetic polymer, and diameter is 0.1~2 μm.
Preferably, the diameter of the microballoon be 0.2 μm, 0.4 μm, 0.6 μm, 0.8 μm, 0.9 μm, 1.0 μm, 1.2 μm, 1.4
μm, 1.6 μm, 1.8 μm.
Third aspect present invention provides a kind of detection method of optical fiber biosensor, comprising the following steps:
S1: the optical fiber biosensor is immersed in the ethanol solution that silylating reagent content is 3~10% and is modified
10~15h, then the optical fiber biosensor after modification is placed in 8~15h in pure water, until the acid in the silylating reagent
Acid anhydride automatic hydrolysis completes the activation of the optical fiber biosensor at carboxyl；
S2: the optical fiber biosensor after the activation is immersed in I solution of antibody carries out antibody modification again, be eventually adding ox
Haemocyanin solution closes remaining carboxyl on optical fiber biosensor；
S3: the optical fiber biosensor after the progress antibody modification is placed in the biological tests sample cell for needing to detect, and
It connect, is detected with wideband light source and spectroanalysis instrument respectively.
Wherein, the optical fiber biosensor is by being separately connected a single-mode fiber as input at coreless fiber both ends
Transmission fiber and output transmission fiber, the input transmission fiber, the coreless fiber and the output transmission fiber are passed through
With drawing cone machine to carry out drawing cone to the coreless fiber after heat sealing machine welding, formed raw based on drawing cone single mode-centreless-single mode optical fiber
Object sensor.
Wherein, the length of the coreless fiber is 5~30mm, and the lumbar vertebrae length of the coreless fiber is 5~15mm, described
The lumbar vertebrae diameter of coreless fiber is 1~10 μm.
Preferably,
The length of the coreless fiber is 10mm, 15mm, 20mm, 25mm；
The lumbar vertebrae length of the coreless fiber is 6mm, 7mm, 8mm, 9mm, 10mm, 11mm, 12mm, 13mm, 14mm；The centreless
The lumbar vertebrae diameter of optical fiber is 2 μm, 3 μm, 4 μm, 5 μm, 6 μm, 7 μm, 8 μm, 9 μm.
Fourth aspect present invention provides a kind of detection method of optical fiber biosensor, comprising the following steps:
S1: the optical fiber biosensor is immersed in the ethanol solution that silylating reagent content is 3~10% and is modified
10~15h, then the optical fiber biosensor after modification is placed in 8~15h in pure water, until the acid in the silylating reagent
Acid anhydride automatic hydrolysis completes the activation of the optical fiber biosensor at carboxyl；
S2: the optical fiber biosensor after the activation is immersed in I solution of antibody carries out antibody modification again, be eventually adding ox
Haemocyanin solution closes remaining carboxyl on optical fiber biosensor；
S3: the optical fiber biosensor after the progress antibody modification is sequentially placed into the biological tests sample cell for needing to detect
And in the microspheres solution of the modification of antibody II, and it connect, is detected with wideband light source and spectroanalysis instrument respectively.
Wherein, the optical fiber biosensor is by being separately connected a single-mode fiber as input at coreless fiber both ends
Transmission fiber and output transmission fiber, the input transmission fiber, the coreless fiber and the output transmission fiber are passed through
With drawing cone machine to carry out drawing cone to the coreless fiber after heat sealing machine welding, formed raw based on drawing cone single mode-centreless-single mode optical fiber
Object sensor.
Wherein, the antibody I for modifying the optical fiber biosensor and the antibody II for modifying the microballoon can be same
Or two different antibody.
Wherein, the length of the coreless fiber is 5~30mm, and the lumbar vertebrae length of the coreless fiber is 5~15mm, described
The lumbar vertebrae diameter of coreless fiber is 1~10 μm.
Preferably,
The length of the coreless fiber is 10mm, 15mm, 20mm, 25mm；
The lumbar vertebrae length of the coreless fiber is 6mm, 7mm, 8mm, 9mm, 10mm, 11mm, 12mm, 13mm, 14mm；The centreless
The lumbar vertebrae diameter of optical fiber is 2 μm, 3 μm, 4 μm, 5 μm, 6 μm, 7 μm, 8 μm, 9 μm.
Wherein, the microballoon is magnetic polymer, and diameter is 0.1~2 μm.
Preferably, the diameter of the microballoon be 0.2 μm, 0.4 μm, 0.6 μm, 0.8 μm, 0.9 μm, 1.0 μm, 1.2 μm, 1.4
μm, 1.6 μm, 1.8 μm.
The principle of the present invention:
Optical fiber biosensor provided by the invention, input transmission fiber, coreless fiber and output transmission fiber pass through heat sealing machine
It carries out drawing cone to several microns to more than ten micron diameters after welding, stronger evanescent wave can be inspired in this way, in its optical fiber appearance
Sample to be tested specific recognition molecules layer is modified in face, is sequentially placed into sample to be tested pond and the table of food-borne pathogens solution later
Face modified specificity identifies in the microspheres solution of molecule.But it is eaten on the food-borne pathogens solution and fibre optical sensor of various concentration
When borne pathogen specific recognition molecules layer combines, cause the refraction index changing of fibre-optical probe outer surface, to generate spectrum
Peak/valley drift.After the optical fiber probe is again dipped into the microspheres solution for be modified with specific recognition molecules, it is attached to light
The microballoon after biomolecule to be measured and modification on fine probe can realize specific binding, therefore can cause outside optical fiber probe again
The change of surface refractive index, so that the peak/valley of spectrum is further drifted about, to increase the sensitivity of sensor.Pass through detection light
The variation of spectrometer peak/valley can realize the measurement of food-borne pathogenic bacteria concentration in food samples.
Beneficial effects of the present invention:
Optical fiber biosensor provided by the invention, sensor surface modify biologic specificity to be measured and identify molecule, later will
The sensor of modified successively immerses in the microspheres solution of sample to be tested pond and modified.Sample to be tested is special with sensor surface
Property identification molecule combine cause fibre optical sensor ambient refractive index to convert, what the microballoon after modification was captured with sensor again
Biomolecule to be measured combines the change again for causing sensor ambient refractive index, to be exaggerated detection signal.Detect interference light
Spectrum variation can realize the measurement of sample to be tested concentration, which has structure simple, quick, high sensitivity excellent
Point.
Detailed description of the invention
It, below will be simple to needing attached drawing to be used to make in embodiment in order to illustrate more clearly of technical solution of the present invention
Singly introduce, it should be apparent that, the accompanying drawings in the following description is only some embodiments of the present invention, corresponds to the general of this field
For logical technical staff, without creative efforts, it is also possible to obtain other drawings based on these drawings.
Fig. 1 is the overall structure diagram for the sensor that the embodiment of the present invention 1 provides；
Fiber-optic sensor probe structural schematic diagram in the sensor that Fig. 2 provides for the embodiment of the present invention 1；
Fig. 3 is that the sensor that present example 1 provides captures the schematic diagram after biology to be measured；
Fig. 4 is that the sensor that present example 2 provides captures the schematic diagram after biology to be measured；
Title corresponding to appended drawing reference in attached drawing are as follows: 1- wideband light source, 2- input transmission fiber, 3- coreless fiber, 4- output
Transmission fiber, 5- spectroanalysis instrument, 6- sample to be tested pond, 7- microballoon, 8- germ, 9- antibody I, 10- antibody II.
Specific embodiment
It is the preferred embodiment of the present invention below, it is noted that for those skilled in the art,
Various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also considered as this hair
Bright protection scope.
Embodiment 1
The present invention provides a kind of optical fiber biosensors, and as depicted in figs. 1 and 2, the optical fiber biosensor includes input
Transmission fiber 2, coreless fiber 3 and output transmission fiber 4；The input transmission fiber 2, the coreless fiber 3 and the output
Transmission fiber 4 is sequentially connected, and the input transmission fiber 2 and wideband light source 1 connect, the output transmission fiber 4 and spectrum point
Analyzer 5 connects；The input transmission fiber 2 and the output transmission fiber 4 are single mode optical fiber；The input transmission fiber 2, institute
It states coreless fiber 3 and the output transmission fiber 4 is tapered fiber；The length of the coreless fiber 3 is 20mm, the centreless light
The lumbar vertebrae length of fibre 3 is 5mm, and the lumbar vertebrae diameter of the coreless fiber 3 is 1 μm.
The detection method of optical fiber biosensor provided in this embodiment, comprising the following steps:
S1: the optical fiber biosensor is immersed in silylating reagent (dihydro -3- (3- (triethoxy silicon substrate) propyl) furan
Mutter -2,5- diketone) content be 5% ethanol solution in carry out modification 10h, then by the optical fiber biosensor after modification
It is placed in 12h in pure water, until the acid anhydrides automatic hydrolysis in the silylating reagent completes the optical fiber biosensor at carboxyl
Activation；
S2: the optical fiber biosensor after the activation is immersed in I 9 solution of antibody carries out antibody modification again, be eventually adding
Bovine serum albumen solution closes remaining carboxyl on optical fiber biosensor；
S3: the optical fiber biosensor after the progress antibody modification is placed in the biological tests sample cell 6 for needing to detect, and
It connect, is detected with wideband light source 1 and spectroanalysis instrument 5 respectively.
Fig. 3 is the schematic diagram immersed sensor after capturing biology to be measured in sample to be tested.
Optical fiber biosensor described in the present embodiment is made by being separately connected a single-mode fiber at 3 both ends of coreless fiber
For input transmission fiber 2 and output transmission fiber 4, the input transmission fiber 2, the coreless fiber 3 and the output are passed
Fibre 4 is lost by, with drawing cone machine to carry out drawing cone to the coreless fiber 3, being formed after heat sealing machine welding and being based on drawing cone single mode-centreless-
Single mode optical fiber biosensor.
Detection of the sensor provided in this embodiment suitable for germ content when more, using Escherichia coli as disease to be detected
Bacterium 8.
Embodiment 2
The present invention provides a kind of optical fiber biosensor, the optical fiber biosensor includes input transmission fiber 2, centreless light
Fibre 3 and output transmission fiber 4；The input transmission fiber 2, the coreless fiber 3 and the output transmission fiber 4 successively connect
It connects, the input transmission fiber 2 and wideband light source 1 connect, and the output transmission fiber 4 and spectroanalysis instrument 5 connect；It is described defeated
Enter transmission fiber 2 and the output transmission fiber 4 is single mode optical fiber；The input transmission fiber 2, the coreless fiber 3 and institute
Stating output transmission fiber 4 is tapered fiber；The length of the coreless fiber 3 is 20mm, and the lumbar vertebrae length of the coreless fiber 3 is
5mm, the lumbar vertebrae diameter of the coreless fiber 3 are 1 μm；The optical fiber biosensor further includes for improving transducer sensitivity
Microballoon 7, the microballoon 7 are magnetic polymer, and diameter is 1 μm.
The detection method of optical fiber biosensor provided in this embodiment, comprising the following steps:
S1: the optical fiber biosensor is immersed in silylating reagent (dihydro -3- (3- (triethoxy silicon substrate) propyl) furan
Mutter -2,5- diketone) content be 5% ethanol solution in carry out modification 10h, then by the optical fiber biosensor after modification
It is placed in 12h in pure water, until the acid anhydrides automatic hydrolysis in the silylating reagent completes the optical fiber biosensor at carboxyl
Activation；
S2: the optical fiber biosensor after the activation is immersed in I 9 solution of antibody carries out antibody modification again, be eventually adding
Bovine serum albumen solution closes remaining carboxyl on optical fiber biosensor；
S3: the optical fiber biosensor after the progress antibody modification is sequentially placed into the biological tests sample cell 6 for needing to detect
In and 7 solution of microballoon modified of antibody II 10 in, and connect, detected with wideband light source 1 and spectroanalysis instrument 5 respectively.
Fig. 4 is the original captured after biology to be measured in the sample to be tested for immerse sensor the microballoon modified containing antibody II
Reason figure.
Optical fiber biosensor described in the present embodiment is made by being separately connected a single-mode fiber at 3 both ends of coreless fiber
For input transmission fiber 2 and output transmission fiber 4, the input transmission fiber 2, the coreless fiber 3 and the output are passed
Fibre 4 is lost by, with drawing cone machine to carry out drawing cone to the coreless fiber 3, being formed after heat sealing machine welding and being based on drawing cone single mode-centreless-
Single mode optical fiber biosensor.
Sensor provided in this embodiment is less suitable for germ content, and the sensor that embodiment 1 provides can not be to germ
Further sensor is immersed in 7 solution of microballoon that antibody II 10 is modified when being monitored and is detected, with Escherichia coli
As germ 8 to be detected, the antibody I 9 for the modification optical fiber biosensor that the present embodiment uses and the antibody II 10 of modification microballoon 7
For two different antibody.
Embodiment 3
The present invention provides a kind of optical fiber biosensor, the optical fiber biosensor includes input transmission fiber 2, centreless light
Fibre 3 and output transmission fiber 4；The input transmission fiber 2, the coreless fiber 3 and the output transmission fiber 4 successively connect
It connects, the input transmission fiber 2 and wideband light source 1 connect, and the output transmission fiber 4 and spectroanalysis instrument 5 connect；It is described defeated
Enter transmission fiber 2 and the output transmission fiber 4 is single mode optical fiber；The input transmission fiber 2, the coreless fiber 3 and institute
Stating output transmission fiber 4 is tapered fiber；The length of the coreless fiber 3 is 10mm, and the lumbar vertebrae length of the coreless fiber 3 is
8mm, the lumbar vertebrae diameter of the coreless fiber 3 are 3 μm；The optical fiber biosensor further includes for improving transducer sensitivity
Microballoon 7, the microballoon 7 are magnetic polymer, and diameter is 0.5 μm.
The detection method of optical fiber biosensor provided in this embodiment, comprising the following steps:
S1: the optical fiber biosensor is immersed in silylating reagent (dihydro -3- (3- (triethoxy silicon substrate) propyl) furan
Mutter -2,5- diketone) content be 4% ethanol solution in carry out modification 12h, then by the optical fiber biosensor after modification
It is placed in 10h in pure water, until the acid anhydrides automatic hydrolysis in the silylating reagent completes the optical fiber biosensor at carboxyl
Activation；
S2: the optical fiber biosensor after the activation is immersed in I 9 solution of antibody carries out antibody modification again, be eventually adding
Bovine serum albumen solution closes remaining carboxyl on optical fiber biosensor；
S3: the optical fiber biosensor after the progress antibody modification is sequentially placed into the biological tests sample cell 6 for needing to detect
In and 7 solution of microballoon modified of antibody II 10 in, and connect, detected with wideband light source 1 and spectroanalysis instrument 5 respectively.
Optical fiber biosensor described in the present embodiment is made by being separately connected a single-mode fiber at 3 both ends of coreless fiber
For input transmission fiber 2 and output transmission fiber 4, the input transmission fiber 2, the coreless fiber 3 and the output are passed
Fibre 4 is lost by, with drawing cone machine to carry out drawing cone to the coreless fiber 3, being formed after heat sealing machine welding and being based on drawing cone single mode-centreless-
Single mode optical fiber biosensor.
Sensor provided in this embodiment is less suitable for germ content, and the sensor that embodiment 1 provides can not be to germ
Further sensor is immersed in 7 solution of microballoon that antibody II 10 is modified when being monitored and is detected, with Escherichia coli
As germ 8 to be detected, the antibody I 9 for the modification optical fiber biosensor that the present embodiment uses and the antibody II 10 of modification microballoon 7
For two different antibody.
Embodiment 4
The present invention provides a kind of optical fiber biosensor, the optical fiber biosensor includes input transmission fiber 2, centreless light
Fibre 3 and output transmission fiber 4；The input transmission fiber 2, the coreless fiber 3 and the output transmission fiber 4 successively connect
It connects, the input transmission fiber 2 and wideband light source 1 connect, and the output transmission fiber 4 and spectroanalysis instrument 5 connect；It is described defeated
Enter transmission fiber 2 and the output transmission fiber 4 is single mode optical fiber；The input transmission fiber 2, the coreless fiber 3 and institute
Stating output transmission fiber 4 is tapered fiber；The length of the coreless fiber 3 is 20mm, and the lumbar vertebrae length of the coreless fiber 3 is
15mm, the lumbar vertebrae diameter of the coreless fiber 3 are 8 μm；The optical fiber biosensor further includes for improving transducer sensitivity
Microballoon 7, the microballoon 7 be magnetic polymer, diameter be 1.5 μm.
The detection method of optical fiber biosensor provided in this embodiment, comprising the following steps:
S1: the optical fiber biosensor is immersed in silylating reagent (dihydro -3- (3- (triethoxy silicon substrate) propyl) furan
Mutter -2,5- diketone) content be 6% ethanol solution in carry out modification 13h, then by the optical fiber biosensor after modification
It is placed in 12h in pure water, until the acid anhydrides automatic hydrolysis in the silylating reagent completes the optical fiber biosensor at carboxyl
Activation；
S2: the optical fiber biosensor after the activation is immersed in I 9 solution of antibody carries out antibody modification again, be eventually adding
Bovine serum albumen solution closes remaining carboxyl on optical fiber biosensor；
S3: the optical fiber biosensor after the progress antibody modification is sequentially placed into the biological tests sample cell 6 for needing to detect
In and 7 solution of microballoon modified of antibody II 10 in, and connect, detected with wideband light source 1 and spectroanalysis instrument 5 respectively.
Optical fiber biosensor described in the present embodiment is made by being separately connected a single-mode fiber at 3 both ends of coreless fiber
For input transmission fiber 2 and output transmission fiber 4, the input transmission fiber 2, the coreless fiber 3 and the output are passed
Fibre 4 is lost by, with drawing cone machine to carry out drawing cone to the coreless fiber 3, being formed after heat sealing machine welding and being based on drawing cone single mode-centreless-
Single mode optical fiber biosensor.
Sensor provided in this embodiment is less suitable for germ content, and the sensor that embodiment 1 provides can not be to germ
Further sensor is immersed in 7 solution of microballoon that antibody II 10 is modified when being monitored and is detected, with Escherichia coli
As germ 8 to be detected, the antibody I 9 for the modification optical fiber biosensor that the present embodiment uses and the antibody II 10 of modification microballoon 7
For two different antibody.
Embodiment 5
The present invention provides a kind of optical fiber biosensor, the optical fiber biosensor includes input transmission fiber 2, centreless light
Fibre 3 and output transmission fiber 4；The input transmission fiber 2, the coreless fiber 3 and the output transmission fiber 4 successively connect
It connects, the input transmission fiber 2 and wideband light source 1 connect, and the output transmission fiber 4 and spectroanalysis instrument 5 connect；It is described defeated
Enter transmission fiber 2 and the output transmission fiber 4 is single mode optical fiber；The input transmission fiber 2, the coreless fiber 3 and institute
Stating output transmission fiber 4 is tapered fiber；The length of the coreless fiber 3 is 15mm, and the lumbar vertebrae length of the coreless fiber 3 is
12mm, the lumbar vertebrae diameter of the coreless fiber 3 are 5 μm；The optical fiber biosensor further includes for improving transducer sensitivity
Microballoon 7, the microballoon 7 be magnetic polymer, diameter be 1 μm.
The detection method of optical fiber biosensor provided in this embodiment, comprising the following steps:
S1: the optical fiber biosensor is immersed in silylating reagent (dihydro -3- (3- (triethoxy silicon substrate) propyl) furan
Mutter -2,5- diketone) content be 8% ethanol solution in carry out modification 14h, then by the optical fiber biosensor after modification
It is placed in 14h in pure water, until the acid anhydrides automatic hydrolysis in the silylating reagent completes the optical fiber biosensor at carboxyl
Activation；
S2: the optical fiber biosensor after the activation is immersed in I 9 solution of antibody carries out antibody modification again, be eventually adding
Bovine serum albumen solution closes remaining carboxyl on optical fiber biosensor；
S3: the optical fiber biosensor after the progress antibody modification is sequentially placed into the biological tests sample cell 6 for needing to detect
In and 7 solution of microballoon modified of antibody II 10 in, and connect, detected with wideband light source 1 and spectroanalysis instrument 5 respectively.
Optical fiber biosensor described in the present embodiment is made by being separately connected a single-mode fiber at 3 both ends of coreless fiber
For input transmission fiber 2 and output transmission fiber 4, the input transmission fiber 2, the coreless fiber 3 and the output are passed
Fibre 4 is lost by, with drawing cone machine to carry out drawing cone to the coreless fiber 3, being formed after heat sealing machine welding and being based on drawing cone single mode-centreless-
Single mode optical fiber biosensor.
Sensor provided in this embodiment is less suitable for germ content, and the sensor that embodiment 1 provides can not be to germ
Further sensor is immersed in 7 solution of microballoon that antibody II 10 is modified when being monitored and is detected, with Escherichia coli
As germ 8 to be detected, the antibody I 9 for the modification optical fiber biosensor that the present embodiment uses and the antibody II 10 of modification microballoon 7
For two different antibody.
The process of optical fiber biosensor fibre-optical probe modification provided by the invention is as follows:
Optical fiber material is silica, and surface contains a large amount of carboxyls, antibody modification to optical fiber surface is carried out in two steps:
The first step is by the way that optical fiber is immersed in silanization ethanol solution (dihydro -3- (3- (triethoxy silicon substrate) propyl) furans -
2,5- diketone) in modified, then the optical fiber after modification is placed in pure water to the acid anhydrides automatic hydrolysis in silylating reagent
At carboxyl.
Second step is to react to fix antibody by the amido bond between the carboxyl of optical fiber surface and the amino of antibody protein
On optical fiber, (1- (3- dimethylamino-propyl) -3- ethyl-carbodiimide hydrochloride)/N- hydroxy amber is specifically first passed through
Acid imide activates optical fiber surface carboxyl groups (generating active ester), and optical fiber is then immersed the optical fiber spy that antibody-solutions obtain antibody modification
Optical fiber is further immersed bovine serum albumen solution by needle, closes the remaining carboxyl of optical fiber surface, is reduced the non-specific of optical fiber and is inhaled
It is attached.
The method that the antibody II 10 being related in the present invention modifies microballoon 7 is the prior art, and details are not described herein.
Only several embodiments of the present invention are expressed for above embodiments, and the description thereof is more specific and detailed, but can not
Therefore limitations on the scope of the patent of the present invention are interpreted as.It should be pointed out that for those of ordinary skill in the art,
Without departing from the inventive concept of the premise, various modifications and improvements can be made, these are all to belong to protection of the invention
Range.Therefore, the scope of protection of the patent of the invention shall be subject to the appended claims.
Claims (10)
1. a kind of optical fiber biosensor, it is characterised in that: the optical fiber biosensor includes input transmission fiber, centreless light
Fine and output transmission fiber；The input transmission fiber, the coreless fiber and the output transmission fiber are sequentially connected, described
Input transmission fiber is connected with wideband light source, and the output transmission fiber is connected with spectroanalysis instrument；The input transmission fiber
It is single mode optical fiber with the output transmission fiber, the coreless fiber is tapered fiber.
2. a kind of optical fiber biosensor according to claim 1, it is characterised in that: the optical fiber biosensor also wraps
Include the microballoon for enhancing transducer sensitivity.
3. a kind of optical fiber biosensor according to claim 2, it is characterised in that: the microballoon is magnetic polymer,
Diameter is 0.1 ~ 2 μm.
4. a kind of optical fiber biosensor described in claim according to claim 1 ~ any one of 3, it is characterised in that: institute
The length for stating coreless fiber is 5 ~ 30mm, and the lumbar vertebrae length of the coreless fiber is 5 ~ 15mm, the lumbar vertebrae diameter of the coreless fiber
It is 1 ~ 10 μm.
5. a kind of detection method of optical fiber biosensor, which comprises the following steps:
S1: the optical fiber biosensor is immersed in the ethanol solution that silylating reagent content is 3~10% and is modified
10~15h, then the optical fiber biosensor after modification is placed in 8~15h in pure water, until the acid in the silylating reagent
Acid anhydride automatic hydrolysis completes the activation of the optical fiber biosensor at carboxyl；
S2: the optical fiber biosensor after the activation is immersed in I solution of antibody carries out antibody modification again, be eventually adding ox
Haemocyanin solution closes remaining carboxyl on optical fiber biosensor；
S3: the optical fiber biosensor after the progress antibody modification is placed in the biological tests sample cell for needing to detect, and
It connect, is detected with wideband light source and spectroanalysis instrument respectively.
6. a kind of detection method of optical fiber biosensor, which comprises the following steps:
S1: by the optical fiber biosensor be immersed in silylating reagent content be 3 ~ 10% ethanol solution in carry out modification 10 ~
15h, then the optical fiber biosensor after modification is placed in 8 ~ 15h in pure water, until the acid anhydrides in the silylating reagent is certainly
It is dynamic to be hydrolyzed into carboxyl, complete the activation of the optical fiber biosensor；
S2: the optical fiber biosensor after the activation is immersed in I solution of antibody carries out antibody modification again, be eventually adding ox
Haemocyanin solution closes remaining carboxyl on optical fiber biosensor；
S3: the optical fiber biosensor after the progress antibody modification is sequentially placed into the biological tests sample cell for needing to detect
And in the microspheres solution of the modification of antibody II, and it connect, is detected with wideband light source and spectroanalysis instrument respectively.
7. a kind of detection method of optical fiber biosensor according to claim 6, it is characterised in that: the microballoon is magnetic
Property polymer, diameter be 0.1 ~ 2 μm.
8. a kind of detection method of optical fiber biosensor according to claim 6, it is characterised in that: modify the optical fiber
The antibody I of biosensor and the antibody II for modifying the microballoon can be same or two different antibody.
9. a kind of detection method of optical fiber biosensor according to claim 5 or 6, it is characterised in that: the optical fiber
Biosensor is by being separately connected a single-mode fiber as input transmission fiber and output transmission light at coreless fiber both ends
The input transmission fiber, the coreless fiber and the output transmission fiber are drawn cone machine by using after heat sealing machine welding by fibre
Drawing cone is carried out to the coreless fiber, is formed and single mode-centreless-single mode optical fiber biosensor is bored based on drawing.
10. a kind of detection method of optical fiber biosensor according to claim 5 or 6, it is characterised in that: the centreless
The length of optical fiber is 5 ~ 30mm, and the lumbar vertebrae length of the coreless fiber is 5 ~ 15mm, and the lumbar vertebrae diameter of the coreless fiber is 1 ~ 10 μ
m。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811258174.2A CN109342330A (en) | 2018-10-26 | 2018-10-26 | A kind of optical fiber biosensor and its detection method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811258174.2A CN109342330A (en) | 2018-10-26 | 2018-10-26 | A kind of optical fiber biosensor and its detection method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109342330A true CN109342330A (en) | 2019-02-15 |
Family
ID=65312128
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811258174.2A Pending CN109342330A (en) | 2018-10-26 | 2018-10-26 | A kind of optical fiber biosensor and its detection method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109342330A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110160960A (en) * | 2019-06-22 | 2019-08-23 | 南昌航空大学 | Micro-nano fiber biosensor and preparation method thereof based on coupler structure |
| CN110530822A (en) * | 2019-08-30 | 2019-12-03 | 河南师范大学 | A method of hollow-core fiber structure measurement refractive index is cascaded using taper coreless fiber |
| CN110927878A (en) * | 2019-11-04 | 2020-03-27 | 桂林电子科技大学 | Non-contact single optical fiber optical tweezers and preparation method thereof |
| CN111257284A (en) * | 2020-02-27 | 2020-06-09 | 南京信息工程大学 | Optical fiber refractive index sensor and preparation method thereof |
| CN111504529A (en) * | 2020-04-24 | 2020-08-07 | 南昌航空大学 | Micro-nano optical fiber nano Newton level mechanical sensor |
| CN113029385A (en) * | 2021-04-14 | 2021-06-25 | 浙江师范大学 | Optical fiber structure for temperature detection, manufacturing method and temperature detection system |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101057134A (en) * | 2004-09-20 | 2007-10-17 | 3M创新有限公司 | Systems and methods for biosensing and microresonator sensors for same |
| CN103245639A (en) * | 2013-04-22 | 2013-08-14 | 中国科学院苏州生物医学工程技术研究所 | WGM (Whispering Gallery Mode) system silicon-based optomagnetic biosensor |
| CN207779880U (en) * | 2018-02-07 | 2018-08-28 | 深圳百奥捷生物科技有限公司 | A kind of photosensitive sensor based on nanometer magnetic bead |
| CN108613950A (en) * | 2018-04-16 | 2018-10-02 | 暨南大学 | Sensitizing type cytochrome c fibre-optical sensing device and method |
-
2018
- 2018-10-26 CN CN201811258174.2A patent/CN109342330A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101057134A (en) * | 2004-09-20 | 2007-10-17 | 3M创新有限公司 | Systems and methods for biosensing and microresonator sensors for same |
| CN103245639A (en) * | 2013-04-22 | 2013-08-14 | 中国科学院苏州生物医学工程技术研究所 | WGM (Whispering Gallery Mode) system silicon-based optomagnetic biosensor |
| CN207779880U (en) * | 2018-02-07 | 2018-08-28 | 深圳百奥捷生物科技有限公司 | A kind of photosensitive sensor based on nanometer magnetic bead |
| CN108613950A (en) * | 2018-04-16 | 2018-10-02 | 暨南大学 | Sensitizing type cytochrome c fibre-optical sensing device and method |
Non-Patent Citations (2)
| Title |
|---|
| 代方: "磁性微球标记纳米光纤生物传感器研究", 《中国优秀硕士学位论文全文数据库 信息科技辑》 * |
| 李明 等: "基于金纳米棒放大的高灵敏度纳米光纤生化传感器", 《光学学报》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110160960A (en) * | 2019-06-22 | 2019-08-23 | 南昌航空大学 | Micro-nano fiber biosensor and preparation method thereof based on coupler structure |
| CN110530822A (en) * | 2019-08-30 | 2019-12-03 | 河南师范大学 | A method of hollow-core fiber structure measurement refractive index is cascaded using taper coreless fiber |
| CN110927878A (en) * | 2019-11-04 | 2020-03-27 | 桂林电子科技大学 | Non-contact single optical fiber optical tweezers and preparation method thereof |
| CN110927878B (en) * | 2019-11-04 | 2022-03-22 | 桂林电子科技大学 | Non-contact single optical fiber optical tweezers and preparation method thereof |
| CN111257284A (en) * | 2020-02-27 | 2020-06-09 | 南京信息工程大学 | Optical fiber refractive index sensor and preparation method thereof |
| CN111504529A (en) * | 2020-04-24 | 2020-08-07 | 南昌航空大学 | Micro-nano optical fiber nano Newton level mechanical sensor |
| CN113029385A (en) * | 2021-04-14 | 2021-06-25 | 浙江师范大学 | Optical fiber structure for temperature detection, manufacturing method and temperature detection system |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109342330A (en) | A kind of optical fiber biosensor and its detection method | |
| Golden et al. | An evanescent wave biosensor. II. Fluorescent signal acquisition from tapered fiber optic probes | |
| Beres et al. | Tapered plastic optical fiber-based biosensor–tests and application | |
| CN101819136B (en) | Human glucose concentration continuous monitoring device based on optical fiber surface plasmon resonance | |
| Rijal et al. | Detection of pathogen Escherichia coli O157: H7 AT 70 cells/mL using antibody-immobilized biconical tapered fiber sensors | |
| CN107085116A (en) | It is a kind of to detect kit of calprotectin and preparation method thereof in human faecal mass sample | |
| CN108572141A (en) | Composite enhanced optical fiber biosensor and bioprotein molecular concentration detection method | |
| CN106896066A (en) | Optical fiber surface plasmon resonance body immune sensing probe and preparation method thereof | |
| CN107741409A (en) | A kind of cancer markers detection means and method based on slant Bragg grating | |
| CN101871881B (en) | Method for detecting protein content in solution | |
| Li et al. | Selective and sensitive Escherichia coli detection based on a T4 bacteriophage‐immobilized multimode microfiber | |
| Socorro et al. | Fiber-optic immunosensor based on an etched SMS structure | |
| CN101126714A (en) | Optical fiber biosensor application method | |
| Salama et al. | Chemiluminescent optical fiber immunosensor for detection of autoantibodies to ovarian and breast cancer-associated antigens | |
| CN101246119A (en) | Optical fiber biological sensing system | |
| Li et al. | Tapered microfiber MZI biosensor for highly sensitive detection of Staphylococcus aureus | |
| CN104359870B (en) | A kind of preparation method of surface plasma body resonant vibration (SPR) bio-sensing chip | |
| CN110160960A (en) | Micro-nano fiber biosensor and preparation method thereof based on coupler structure | |
| Kumar et al. | Detection of botulinum toxin using an evanescent wave immunosensor | |
| Han et al. | SARS-CoV-2 spike protein detection using slightly tapered no-core fiber-based optical transducer | |
| CN201060167Y (en) | Microstructure optical fibre sensing probe | |
| CN109612967A (en) | Micro-nano fiber biosensor and preparation method thereof based on Mach-Zehnder interference | |
| Li et al. | Gold/zinc oxide nanoparticles functionalized tapered SMF-MMF-SMF-based sensor probe for uric acid detection | |
| CN207147972U (en) | A kind of Detection of immuno-protein system based on optical fiber surface plasmon resonance | |
| CN109342716A (en) | Lack mould-single mode structure D-type optical fiber sensor based on single mode- |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190215 |