CN109337889B - 一种酶活提高的金属β-内酰胺酶突变体及其构建方法 - Google Patents
一种酶活提高的金属β-内酰胺酶突变体及其构建方法 Download PDFInfo
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- CN109337889B CN109337889B CN201811601230.8A CN201811601230A CN109337889B CN 109337889 B CN109337889 B CN 109337889B CN 201811601230 A CN201811601230 A CN 201811601230A CN 109337889 B CN109337889 B CN 109337889B
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
- C12N9/86—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in cyclic amides, e.g. penicillinase (3.5.2)
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- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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- C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
- C12Y305/02—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amides (3.5.2)
- C12Y305/02006—Beta-lactamase (3.5.2.6)
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Abstract
Description
菌号 | 氨基酸突变位点 | 相对活力(%) |
原始菌株 | —— | 100% |
金属β-内酰胺酶P151T | P151T | 452% |
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CN201811601230.8A CN109337889B (zh) | 2018-12-26 | 2018-12-26 | 一种酶活提高的金属β-内酰胺酶突变体及其构建方法 |
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CN201811601230.8A CN109337889B (zh) | 2018-12-26 | 2018-12-26 | 一种酶活提高的金属β-内酰胺酶突变体及其构建方法 |
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CN109337889A CN109337889A (zh) | 2019-02-15 |
CN109337889B true CN109337889B (zh) | 2020-10-02 |
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WO2024012487A1 (en) * | 2022-07-14 | 2024-01-18 | The Hong Kong Polytechnic University | Biosensor comprising a modified beta-lactamase and uses thereof |
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WO2001019971A1 (en) * | 1999-09-17 | 2001-03-22 | Smithkline Beecham Corporation | Novel imp-1 metallo beta-lactamase enzyme |
CN101139581A (zh) * | 2006-09-05 | 2008-03-12 | 上海生物芯片有限公司 | 突变的SHV型β-内酰胺酶、其基因序列及应用 |
JP6685925B2 (ja) * | 2014-04-17 | 2020-04-22 | シンセティック バイオロジクス,インコーポレイテッド | 治療用として改善された特性を有するβ−ラクタマーゼ |
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Effective date of registration: 20191009 Address after: 200000 d2666, No. 200, Huancheng Road, Jiading District, Shanghai Applicant after: Shanghai Junjie Biochemistry Technology Co., Ltd. Address before: 510070 Wanbao North Street, Panyu District, Guangzhou, Guangdong Province, No. 1 Applicant before: Guangzhou Baiyunshan Baidi Biotechnology Co., Ltd. |
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Effective date of registration: 20191029 Address after: 510000 No.1, North Wanbao street, Panyu District, Guangzhou City, Guangdong Province Applicant after: Guangzhou Baiyunshan Baidi Biotechnology Co., Ltd. Applicant after: Shanghai Junjie Biochemistry Technology Co., Ltd. Address before: 200000 d2666, No. 200, Huancheng Road, Jiading District, Shanghai Applicant before: Shanghai Junjie Biochemistry Technology Co., Ltd. |
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