CN109331061B - Kuh-seng flavonoid effective part with tyrosinase inhibitory activity and antibacterial activity, preparation method and application thereof - Google Patents
Kuh-seng flavonoid effective part with tyrosinase inhibitory activity and antibacterial activity, preparation method and application thereof Download PDFInfo
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- CN109331061B CN109331061B CN201811346769.3A CN201811346769A CN109331061B CN 109331061 B CN109331061 B CN 109331061B CN 201811346769 A CN201811346769 A CN 201811346769A CN 109331061 B CN109331061 B CN 109331061B
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Abstract
The invention discloses a kuh-seng flavonoid effective part with tyrosinase inhibitory activity and antibacterial activity and a preparation method thereof, wherein kuh-seng dregs are used as raw materials, waste materials are changed into things of value, ethanol is added for reflux extraction, filtration is carried out, filtrate is dried to obtain ethanol extract, after the ethanol extract is dissolved by methanol, the ethanol extract is loaded on AB-8 type macroporous adsorption resin, then gradient elution is carried out by ethanol with the concentration of 10%, 20%, 30%, 40%, 50%, 60%, 70% and 80%, and 50-70% of elution part is collected. The effective part prepared by the invention has obvious inhibitory activity on tyrosinase, has obvious antibacterial effect on staphylococcus aureus and streptococcus agalactiae, has good prevention and treatment effects on human body pigmentation diseases caused by excessive melanin, and has obvious and wide biological activity.
Description
Technical Field
The invention relates to a traditional Chinese medicine extract, in particular to a flavone effective part with tyrosinase inhibition and bacteriostasis functions in sophora flavescens decoction dregs, and belongs to the technical field of medicines.
Background
Radix Sophorae Flavescentis, a dry root of Sophoraflaviscens ait. of Leguminosae, is bitter in taste and cold in nature, and has effects of clearing heat, eliminating dampness, killing parasites, and promoting urination. The lightyellow sophora root is widely distributed and planted in most places in China; the main productions are Shanxi, Henan, Hebei, inner Mongolia and so on. Modern pharmacological studies show that alkaloid components in the radix sophorae flavescentis have biological activities of resisting tumors, hepatitis B viruses, inflammation, bacteria and insects and the like, and are widely applied to development of products such as pharmaceutical preparations, biological pesticides, veterinary drugs and the like. The market demand of the sophora flavescens as a common bulk Chinese medicinal material variety is increased year by year, and meanwhile, the quantity of solid wastes and byproducts generated in the preparation process is huge, and the solid wastes and the byproducts are discarded without an effective utilization way, so that the resource waste is caused, and the green and sustainable development of the sophora flavescens resource industry is not facilitated for a long time.
At present, alkaloid components in sophora flavescens are mostly used as raw materials in the related preparation industry of sophora flavescens, and water or acidic aqueous solution is mostly used as an extraction solvent when the alkaloid components are extracted. Because alkaloid components in the sophora flavescens are easy to dissolve in an acidic aqueous solution, while flavonoid components are poor in water solubility and are not easy to dissolve under an acidic condition, most of the alkaloid components are reserved in the herb residues after alkaloid extraction. Researches show that the flavonoids contained in radix sophorae flavescentis are mostly isovaleryl-substituted flavanone and flavanonol, and a small amount of flavonoids, isoflavones, pterocarpans and echinenone.
Tyrosinase is a key enzyme for melanin synthesis, and the expression and activity of tyrosinase are closely related to browning of fruits and vegetables and human body pigmentation diseases. Therefore, the tyrosinase inhibitor can be used as a melanin inhibitor to be applied to the fields of food, cosmetics, medicines and the like.
Disclosure of Invention
The purpose of the invention is: the radix sophorae flavescentis dregs after the alkaloid is extracted from the radix sophorae flavescentis are fully utilized, the effective parts of the total flavonoids in the radix sophorae flavescentis dregs are extracted through an optimal optimization process method, and experimental researches show that the effective parts of the total flavonoids in the radix sophorae flavescentis dregs prepared by the method have obvious tyrosinase inhibitory activity and bacteriostatic activity, and are high in total flavonoids content and few in impurities.
The technical scheme is as follows: the invention adopts the following technical scheme:
an effective component of radix Sophorae Flavescentis flavonoid with tyrosinase inhibitory activity and antibacterial activity is extracted from radix Sophorae Flavescentis residue, and the effective component comprises flavonoid compound with the following structure:
the preparation method of the kuh-seng flavonoid effective part with tyrosinase inhibitory activity and antibacterial activity, which is disclosed by the invention, comprises the following steps of:
(1) drying and crushing the sophora flavescens dregs, adding 40-80% ethanol according to the material-liquid ratio of 1g to 10-50 ml, carrying out reflux extraction for 1-2 times, each time for 2 hours, and concentrating the extracting solution under reduced pressure to obtain a dry substance;
(2) dissolving the dried substance obtained in the step (1) with 95% ethanol, and filtering;
(3) and (3) concentrating the filtrate obtained in the step (2) under reduced pressure to obtain a dry substance, adding a small amount of methanol, ultrasonically dissolving, slowly adsorbing on a macroporous adsorption resin column, sequentially eluting with ethanol with volume concentration of 10%, 20%, 30%, 40%, 50%, 60%, 70% and 80% which is 5-8 times of the volume of the column, and collecting the 50-70% ethanol elution part.
As a preferred embodiment, the preparation method of the flavonoid effective fraction having tyrosinase inhibitory activity and antibacterial activity described above is characterized by comprising the following steps:
and (3) the macroporous adsorption resin column in the step (3) is AB-8 type, and finally, an ethanol elution part with the volume concentration of 60% is collected.
A whitening emulsion comprising the flavonoid effective fraction of Sophora flavescens Aiton having tyrosinase inhibitory activity and antibacterial activity according to claim 1.
An agent for inhibiting browning of fruits and vegetables, which comprises the flavonoid effective fraction of Sophora flavescens having tyrosinase inhibitory activity and antibacterial activity according to claim 1.
A medicament for preventing and treating pigmentation in a human body caused by melanin abnormality, which comprises the effective fraction of the flavonoid represented by claim 1 having tyrosinase inhibitory activity and antibacterial activity.
The kuh-seng flavonoid effective part with tyrosinase inhibitory activity and antibacterial activity is applied to preparing fruit and vegetable enzymatic browning inhibitors, whitening cosmetics and bacteriostats and preventing and treating human body pigmentation diseases or melanoma caused by melanin abnormality.
The above-mentioned application, the dosage form of the medicine or cosmetic includes solid powder, aqueous solution, alcoholic solution, emulsion, mask, cataplasm, injection, infusion solution, powder injection, granule, tablet, electuary, powder, oral liquid, sugar-coated agent, film-coated tablet, enteric-coated tablet, capsule, buccal agent, pill, paste, pellet, spray, drop pill, oral disintegrating agent or pellet.
The invention has the advantages that:
the method changes waste into valuable, and makes full use of the sophora flavescens dregs after the alkaloid is extracted from the sophora flavescens, and extracts the effective part with high content of total flavonoids in the sophora flavescens dregs by an optimal optimization process method. The extraction process of the invention is reasonable, the cost is low, and the invention can be used for industrial production.
Drawings
FIG. 1 is a UPLC-MS comparison of total ion current for different concentrations of ethanol elution sites;
in the figure, A: eluting with 10% ethanol; b: 20% ethanol elution fraction; c: eluting with 30% ethanol; d: eluting with 40% ethanol; e: eluting with 50% ethanol; f: eluting with 60% ethanol; g: eluting with 70% ethanol; h: 80% ethanol elution part.
Detailed Description
The substance and advantageous effects of the present invention will be described in further detail with reference to examples, which are provided only for illustrating the present invention and not for limiting the present invention. In addition, after reading the teaching of the present invention, those skilled in the art can make various changes or modifications to the invention, and these equivalents also fall within the scope of the claims appended to the present application.
EXAMPLE 1 preparation of effective fractions
Extracting 10 kg radix Sophorae Flavescentis residue (15 times volume of radix Sophorae Flavescentis residue obtained after extracting alkaloid by percolating with 1% acetic acid water solution) with 8 times of 80% ethanol under ultrasonic wave for 3 times, 1 hr each time, concentrating under reduced pressure to dry, dissolving the dried product with 95% ethanol, filtering, concentrating the filtrate under reduced pressure, drying, adding methanol for ultrasonic dissolution, loading onto macroporous resin AB-8 chromatographic column, eluting with 8 volumes of 10%, 20%, 30%, 40%, 50%, 60%, 70% and 80% ethanol in sequence, and collecting each eluted part.
The invention adopts the following method to measure the flavone content of the eluent with different volume concentrations of ethanol (10 percent to 80 percent).
Detection conditions of UPLC-TOF-MS/MS:
the instrument comprises the following steps: AB Sciex
5600+ time-of-flight mass spectrometer, Shimadzu LC10A high performance liquid chromatograph
A chromatographic column: an ACQUITY UPLC BEH C18 column (100 mm. times.2.1 mm,1.7 μm),
mobile phase: 0.1% aqueous formic acid (a) -acetonitrile (B), gradient elution: 0 → 15min, 5% B → 50% B; 15 → 20min, 50% B → 80% B; 20 → 25min, 80% B → 80% B; the flow rate is 0.4mL/min, and the column temperature is 40 ℃; the amount of sample was 2. mu.L.
Mass spectrum conditions: a positive and negative ion mode; and (4) CUR: 35.000 psi; GS 1: 55.000 psi; GS 2: 55.000 psi; TEM: 550.000 deg.C; CE: 10.000V (negative ion mode); DP: 60.000V (negative ion mode); the scanning ranges m/z 100-.
The results are shown in FIG. 1: 10%, 20%, 30%, 40%, 50%, 60%, 70% and 80% of the ethanol eluates contain the following flavones:
the total flavone content of the 10% ethanol eluate was 12.5%. Mainly comprises the following components: 4-O- β -D-glucopyranoside, 3 '-hydroxy-kushenol O, 3',4 '-dihydroxy-isoflavone-7-O- β -D-glucopyranoside, 2, 3-dihydroxy-4' -methoxyflavanone 7-O- β -D-apiose- (1 → 6) - β -D-glucopyranoside;
the total flavone content of the 20% ethanol eluate was 18.4%. Mainly comprises the following components: cheilone-4-O- β -D-glucopyranoside, 3' -hydroxy-kushenol O, 3',4' -dihydroxy-isoflavone-7-O- β -D-glucopyranoside, 2, 3-dihydroxy-4 ' -methoxyflavanone 7-O- β -D-apiose- (1 → 6) - β -D-glucopyranoside, kuschecarin D, biochanin a, 7-methoxy-4 ' -hydroxyisoflavone, isoxanthohumol, homosophoricine;
the total flavone content of the 30% ethanol eluent is 16.3%. Mainly comprises the following components: mosoxanone-4-O-beta-D-glucopyranoside, 3 '-hydroxy-kushenol O, 3',4 '-dihydroxy-isoflavone-7-O-beta-D-glucopyranoside, 5,4' -dihydroxy-isoflavone-7-O-beta-D-xylose- (1 → 6) -beta-D-glucopyranoside, daidzein-7-O-beta-D-xylose- (1 → 6) -beta-D-glucopyranoside, 2-hydroxy-6- [2- (4-hydroxyphenyl) -2-carbonyl ] benzoic acid-3-O-beta-D-xylose- (1 → 6) -beta-D-glucopyranoside, kurarinol J, 7-hydroxy-4 '-methoxy-flavanone-3' -O- β -D-glucopyranoside, trifoliosid, 2, 3-dihydroxy-4 '-methoxy-flavanone-7-O- β -D-xylose- (1 → 6) - β -D-glucopyranoside, kurarinol O, biochanin a, kurarinol H, kurarinol K, 4' -hydroxy-isoflavone-7-O- β -D-apiose- (1 → 6) - β -D-glucopyranoside, garicin, kurarinol N, kurarinone, sophoricoside G;
the total flavone content of 40% ethanol eluate was 26.7%. Mainly comprises the following components: 3 '-hydroxy-kushenol O, 5,4' -dihydroxy-isoflavone-7-O-beta-D-xylose- (1 → 6) -beta-D-glucopyranoside, daidzein-7-O-beta-D-xylose- (1 → 6) -beta-D-glucopyranoside, kurarinol J, 7-hydroxy-4 '-methoxy-flavanone-3' -O-beta-D-glucopyranoside, pterocarpin, pseudoobtigenin-7-O-beta-D-xylose- (1 → 6) -beta-D-glucopyranoside, 2, 3-dihydroxy-4 '-methoxyflavanone-7-O-beta-D-xylose- (2, 3-dihydroxy-4' -methoxyflavanone-7-O-beta-D-xylopyranoside 1 → 6) - β -D-glucopyranoside, kurarinol O, 4' -methoxy-isoflavone-7-O- β -D-apiose- (1 → 6) - β -D-glucopyranoside, kushecarpin D, daidzein, biochanin a, kurarinol H, isodehydroicaritin, kurarinol W, kurarinol K, kurarinol P, kurarinol glycol, 7-methoxy-4 ' -hydroxyisoflavone, kurarinol G, naringenin, isoxanthohumol, kurarinol Q, homosophoricol, kurarinol N, kurarinone, kurarinol D, sophoricoside G, 2' -methoxykurarinone;
the total flavone content of the 50% ethanol eluate was 35.1%. Mainly comprises the following components: 7-hydroxy-4 '-methoxy-flavanone-3' -O-beta-D-glucopyranoside, pseudooblitigin-7-O-beta-D-xylose- (1 → 6) -beta-D-glucopyranoside, 2, 3-dihydroxy-4 '-methoxy-flavanone-7-O-beta-D-xylose- (1 → 6) -beta-D-glucopyranoside, kurarinol O, 4' -methoxy-isoflavone-7-O-beta-D-apiose- (1 → 6) -beta-D-glucopyranoside, pterocarpin, luteolin, daidzein, biochanin A, kurarinol H, isodehydroicaritin, kurarinol W, kurarinol K, kurarinol P, 4 '-hydroxy-isoflavone-7-O-beta-D-apiose- (1 → 6) -beta-D-glucopyranoside, (2R,3R) -8-isopentenyl-7, 2',4 '-trihydroxy-5-methoxyflavanonol, kurarinol, pseudobigenin, luteolin, 7-methoxy-4' -hydroxyisoflavone, kurarinol G, naringenin, isoxanthohumol, kurarinol Q, rikanone G, quercetin, homosophoricol, sophorafinol, 5-methoxy-7, 2',4' -trihydroxy-8-isopentenyl flavone, kurarinol V, kurarinol N, sophoflavone B, kurarinone, 5-methyl kurarinol C, kurarinol X, kurarinol S, anhydroicaritin, neokurarinol, kurarinol R, kurarinol C, kurarinol D, sophoricone flavanone G, (2S) -7,4 '-dihydroxy-5-methoxy-8- (gamma, gamma-dimethylallyl) -flavanone, citrusinol, 8-lavandiol, kuraridine, norkurarinone, 5, 7-dihydroxy-8-lavandione, kurarinol E, richardnone A, kurarinol M, kurarinol A, 2' -methoxykurarinone;
the total flavone content of the 60% ethanol eluate was 41.2%. Mainly comprises the following components: luteolin, daidzein, biochanin A, kurarinol H, isodehydroicaritin, kurarinol W, kurarinol K, kurarinol P, 4 '-hydroxy-isoflavone-7-O-beta-D-apiose- (1 → 6) -beta-D-glucopyranoside, (2R,3R) -8-isopentenyl-7, 2',4 '-trihydroxy-5-methoxydihydroflavonol, kurarinol, pseudobiotinin, luteolin, 7-methoxy-4' -hydroxyisoflavone, kurarinol G, naringenin, isoxanthohumol, kurarinol Q, richardinone G, quercetin, homosophoricilin, sophorafinol, 5-methoxy-7, 2',4' -trihydroxy-8-isopentenyl flavone, kurarinol V, kurarinol N, alfalfa lactone, 5, 7-dihydroxy-8 (R, R-dimethylallyl) pyrone, sophorflavone B, kurarinone, 5-methylkurarinol C, kurarinol X, kurarinol S, anhydroicaritin, kurarinol F, neokurarinol, kurarinol R, kurarinol C, kurarinol D, sophoraflavanone G, sophoraflavanone B, kurarinol L, kurarinol U, (2S) -7,4 '-dihydroxy 5-methoxy-8- (gamma, gamma-dimethylallyl) -flavanone, citrinol, 8-lavandiol, kuraridine, norkurarinone, 5, 7-dihydroxy-8-lavandula chromone, neonol E, richardnone A, kurarinol M, kurarinol A, 2' -methoxykurarinone, kurarinol B;
the total flavone content of the 70% ethanol eluate was 37.3%. Mainly comprises the following components: biochanin A, kurarinol H, kurarinol K, kurarinol P, 4 '-hydroxy-isoflavone-7-O-beta-D-apiose- (1 → 6) -beta-D-glucopyranoside, kurarinol, pseudobiotinin, luteolin, 7-methoxy-4' -hydroxyisoflavone, kurarinol G, naringenin, isoxanthohumol, kurarinol Q, richinone G, quercetin, sophoricol, 5-methoxy-7, 2',4' -trihydroxy-8-isopentenyl flavone, kurarinol V, kurarinol N, medicago lactone, 5, 7-dihydroxy-8 (r, r-dimethylallyl) pyrone, sophoaflavone B, kurarinone, 5-methylkurarinol C, kurarinol X, kurarinol S, anhydroicaritin, kurarinol F, neokurarinol, kurarinol R, kurarinol C, kurarinol D, sophoricoside G, sophoricoside B, kurarinol L, kurarinol U, (2S) -7,4 '-dihydroxy-5-methoxy-8- (γ, γ -dimethylallyl) -flavanone, citrusinol, 8-lavandiol, kuraridine, norkurarinone, 5, 7-dihydroxy-8-lavandinone, kurarinol E, richardnone a, kurarinol M, kurarinol a, 2' -methoxykurarinone, kurarinol B;
the total flavone content of 80% ethanol eluent is 32.5%. Mainly comprises the following components: kurarinol K, kurarinol P, kurarinol, pseudobiotagenin, luteolin, 7-methoxy-4 '-hydroxyisoflavone, naringenin, isoxanthohumol, kurarinol Q, quercetin, garbinin, sophorafinol, 5-methoxy-7, 2',4 '-trihydroxy-8-prenylflavone, kurarinol V, kurarinol N, medicago lactone, 5, 7-dihydroxy-8 (R, R-dimethylallyl) pyrone, sophorafenone B, kurarinone, 5-methylkurarinol C, kurarinol X, kurarinol S, anhydroicaritin, kurarinol F, neokurarinol, kurarinol R, kurarinol C, kurarinol D, sophoricoside G, sophorico flavanone B, kurarinol L, kurarinol U, (2S) -7,4' -dihydroxy-5-methoxy-8- (gamma), gamma-dimethylallyl) -flavanone, citrusinol, 8-lavender kaempferol, kuraridine, norkurarinone, 5, 7-dihydroxy-8-lavender chromone, kurarinol E, rikainone A, kurarinol M, kurarinol A, 2' -methoxykurarinone, and kurarinol B.
Comparing the detection results to obtain: the 60% ethanol elution fraction is particularly preferred because the active ingredient of flavone contained in the 60% ethanol elution fraction is rich and high in content, and contains few impurities.
Example 2 evaluation of tyrosinase inhibitory Activity of the effective fraction of Sophora flavescens residue extract
Dissolving the 10-80% ethanol eluate prepared in example 1 in methanol respectively to prepare 5mg/mL solution, and diluting to 2.500, 1.250, 0.625, 0.312, 0.156, and 0.078 mg/mL-1Six concentrations. Preparation of 5mg/mL-1The L-tyrosine solution of (2), 0.1mol/L phosphate buffer solution, 15mmol/L L-tyrosine solution, 0.25 mg/mL-1Tyrosinase, etc. Placing each group of reaction solution in a test tube, keeping the temperature in a water bath at 37 ℃ for 10min, adding 0.50mL of L-tyrosine solution respectively, reacting for 15min, quickly transferring into a cuvette, measuring the absorbance of each group at 475nm, and calculating the inhibition rate of the extraction solution with different polarity parts on tyrosinase.
The enzyme activity inhibition rate was [ (A2-A1) - (B2-B1) ]/(A2-A1) × 100%
A1 is the absorption value of no inhibitor at 0 min; a2 is the absorption value after 20min without inhibitor;
b1 is the absorption value of inhibitor added at 0 min; b2 is the absorption value of the inhibitor added after 20 min.
The inhibition rate and IC50 value of each effective part of radix Sophorae Flavescentis residue extract at six concentrations are shown in Table 1,
TABLE 1 tyrosinase activity inhibition rate and IC50 value of six concentrations of effective components of radix Sophorae Flavescentis residue extract
Example 3 evaluation of bacteriostatic Activity of effective fractions of Sophora flavescens Aiton residue extract
Dissolving the 10-80% ethanol eluate prepared in example 1 in methanol respectively to prepare 5mg/mL solution, and diluting to 2.500, 1.250, 0.625, 0.312, 0.156, and 0.078 mg/mL-1Six concentrations. Preparing qualitative filter paper into 6mm round piece with a puncher, placing into a dry culture dish, sterilizing with high pressure steam at 121 deg.C for 20min, and placing the filter paper piece into a sterilized culture dish. Dripping 5 μ L of medicinal liquid into each paper sheet, and drying in a drying oven to remove solvent. And uniformly coating the diluted bacteria liquid on LB agar by using a sterilized cotton swab, taking sterilized paper sheets, sequentially clamping the sterilized paper sheets by using a sterilized forceps, sequentially dipping the sterilized paper sheets on the agar, standing for 30min, culturing in an incubator at 37 ℃ for 24h, observing the size of a bacteriostatic zone, calculating an average value, and calculating the minimum bacteriostatic concentration (MIC). The results of the inhibitory activity of different elution parts of the sophora flavescens decoction dregs on staphylococcus aureus and streptococcus agalactiae are shown in a table 2.
TABLE 2 inhibitory Activity of effective fractions of Sophora flavescens Aiton residue extract against Staphylococcus aureus and Streptococcus agalactiae
The experimental results in tables 1 and 2 show that the extract obtained by extracting the sophora flavescens dregs can be subjected to AB-8 type macroporous resin chromatography, and particularly eluted by using ethanol with the volume concentration of 60%, so that the total flavone containing 8 different structural types can be obtained, and the content of the total flavone can reach more than 80%. The tyrosinase inhibitory activity and the antibacterial activity show that 60 percent of ethanol eluted substance has the best activity at the same time, and very good technical effect is achieved!
Example 4 preparation of whitening emulsion
1. Prepared according to the formulation of table 3: mixing the extracts in phase A, adding into 70 deg.C heated polyethylene glycol 400, adding glycerol and deionized water, and mixing;
dissolving B phase at 80 deg.C, and mixing; adding phase A into phase B, homogenizing for 10min, cooling to 50 deg.C, adding phase C, homogenizing for 5min, aging for 24 hr, and packaging.
TABLE 3 whitening lotion formulation table
2. Effect on B16F10 cell proliferation
The influence of whitening emulsion on B16F10 cell proliferation is observed through MTT experiment, the dosage of the whitening emulsion is set to be 5, 10, 20 and 40ug/ml according to the effective part of sophora flavescens decoction dregs, and the dosage of the whitening emulsion is set to be 5, 10, 20 and 40ug/ml according to the contrast medicament: the MTT experimental result of Table 4 shows that the whitening emulsion has a strong inhibition effect on B16F10 cell proliferation, and has a good proportional relation within a dosage range of 5-80 ug/ml, and after the administration of the whitening emulsion is carried out for 24 hours, the cell activity of a normal cell group is obviously weakened along with the prolonging of the culture time, which shows that the traditional Chinese medicine compound extracting solution has a strong inhibition effect on melanoma cells.
Table 4 effect on B16F10 cell proliferation (n-5,
)
comparison with cell control group*P<0.05,**P<0.01
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (1)
1. The application of effective component of kuh-seng flavonoid with tyrosinase inhibitory activity and antibacterial activity; the application is the application in preparing whitening cosmetics, the application in preparing bacteriostatic agents and the application in preparing medicaments for preventing and treating human body pigmentation diseases or melanoma diseases caused by abnormal melanin;
the effective part of the kuh-seng flavonoid is prepared by the following method:
(1) drying and crushing the sophora flavescens dregs, adding 40-80% ethanol according to the material-liquid ratio of 1g to 10-50 ml, carrying out reflux extraction for 1-2 times, each time for 2 hours, and concentrating the extracting solution under reduced pressure to obtain a dry substance;
(2) dissolving the dried substance obtained in the step (1) with 95% ethanol, and filtering;
(3) and (3) concentrating the filtrate obtained in the step (2) under reduced pressure to obtain a dried substance, adding a small amount of methanol, ultrasonically dissolving, slowly adsorbing on an AB-8 type macroporous adsorption resin column, sequentially eluting with ethanol with volume concentration of 10%, 20%, 30%, 40%, 50%, 60%, 70% and 80% which is 5-8 times of the volume of the column, and collecting a 60% ethanol elution part.
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