CN109329543A - A kind of production technology using lactic acid bacteria and enzyme preparation preservation fruit embryo - Google Patents

A kind of production technology using lactic acid bacteria and enzyme preparation preservation fruit embryo Download PDF

Info

Publication number
CN109329543A
CN109329543A CN201811257476.8A CN201811257476A CN109329543A CN 109329543 A CN109329543 A CN 109329543A CN 201811257476 A CN201811257476 A CN 201811257476A CN 109329543 A CN109329543 A CN 109329543A
Authority
CN
China
Prior art keywords
raw material
fresh fruit
lactic acid
acid bacteria
enzyme preparation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201811257476.8A
Other languages
Chinese (zh)
Inventor
陈宇
谢丹
郭敏佳
郭婷婷
杨丹娜
沈安妮
黄妙云
蔡醉冰
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to CN201811257476.8A priority Critical patent/CN109329543A/en
Publication of CN109329543A publication Critical patent/CN109329543A/en
Pending legal-status Critical Current

Links

Classifications

    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23GCOCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
    • A23G3/00Sweetmeats; Confectionery; Marzipan; Coated or filled products
    • A23G3/34Sweetmeats, confectionery or marzipan; Processes for the preparation thereof
    • A23G3/36Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds
    • A23G3/48Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds containing plants or parts thereof, e.g. fruits, seeds, extracts
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23BPRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
    • A23B7/00Preservation or chemical ripening of fruit or vegetables
    • A23B7/14Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
    • A23B7/153Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
    • A23B7/154Organic compounds; Microorganisms; Enzymes
    • A23B7/155Microorganisms; Enzymes; Antibiotics
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/20Removal of unwanted matter, e.g. deodorisation or detoxification
    • A23L5/21Removal of unwanted matter, e.g. deodorisation or detoxification by heating without chemical treatment, e.g. steam treatment, cooking
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/20Removal of unwanted matter, e.g. deodorisation or detoxification
    • A23L5/25Removal of unwanted matter, e.g. deodorisation or detoxification using enzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/30Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation
    • A23L5/32Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation using phonon wave energy, e.g. sound or ultrasonic waves
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/30Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation
    • A23L5/34Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation using microwaves
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Botany (AREA)
  • Inorganic Chemistry (AREA)
  • Preparation Of Fruits And Vegetables (AREA)
  • Storage Of Fruits Or Vegetables (AREA)

Abstract

The present invention proposes a kind of production technology using lactic acid bacteria and enzyme preparation preservation fruit embryo, comprising the following steps: selection fresh fruit raw material, cleaning, pretreatment, high temperature, which scald, to be boiled, rinses, configuring lactic acid bacteria soak, configuration enzyme preparation, compounding maceration extract, ultrasonic permeability dipping and freeze-day with constant temperature;The present invention proposes to can use the special physiological activity of lactic acid bacteria by lactic acid bacteria soak to keep the microecological balance of fruit preservation environment, it can inhibit raw material preserving process spoilage organisms growth and breeding, de- hardship can be carried out to fresh fruit raw material by the naringinase in enzyme preparation, improve the mouthfeel of fresh fruit raw material, depickling can be carried out to fresh fruit raw material by carboxypeptidase, the problem of avoiding fresh fruit acidity excessive growth spoilage organisms, being handled by ultrasonic permeability can be improved absorption of the fresh fruit to lactic acid bacteria soak and enzyme preparation, permeability is more preferable, carrying out freeze-day with constant temperature by microwave vacuum dryer can be improved drying efficiency, uniform drying, it is produced suitable for large batch of fruit embryo.

Description

A kind of production technology using lactic acid bacteria and enzyme preparation preservation fruit embryo
Technical field
The present invention relates to preserved fruit production field more particularly to a kind of production works using lactic acid bacteria and enzyme preparation preservation fruit embryo Skill.
Background technique
Preserved fruit is Han nationality's traditional food with national characters, has more than 2000 years history so far, preserved fruit is by fresh Fruits and vegetables are raw material, and the food being processed into after marinated with sugar or honey, the presence of preserved foods solves fresh fruit of vegetables can not Long-term preservation leads to the problem of wasting of addling, and preserved fruit, which is made, in fresh fruit of vegetables can extend storage life, while the nutritive value of preserved fruit Also very high, it can promote to digest after edible, there is the effect of appetite-stimulating and indigestion-relieving.
Color protection can be added when to fresh fruit of vegetables pretreatment of raw material in process of production in production technology in existing preserved fruit Agent and preservative save the color of fresh fruit of vegetables raw material, but color stabilizer and preservative are chemical addition agent, can destroy new fresh fruit The nutritional benefits ingredient such as vitamin, tartaric acid inside vegetable raw material causes fruit embryo nutrition value and is lost, the fruit embryo containing additive Long-term consumption can endanger the health of people, while make after fresh fruit of vegetables pretreatment of raw material in existing preserved fruit production process At fruit embryo storage life it is short, often need to carry out sugaring processing immediately after fruit embryo is made, otherwise fruit embryo is apt to deteriorate, while fruit Embryo is higher to environmental requirement, easily growth harmful bacteria, can improve production cost, the economic benefit of generation is more low.Therefore, originally Invention proposes a kind of production technology using lactic acid bacteria and enzyme preparation preservation fruit embryo, to solve shortcoming in the prior art.
Summary of the invention
In view of the above-mentioned problems, the present invention propose by lactic acid bacteria soak can use the special physiological activity of lactic acid bacteria come The microecological balance for keeping fruit preservation environment, can inhibit the production of raw material preserving process spoilage organisms growth and breeding and spoilage product It is raw, de- hardship can be carried out to fresh fruit raw material by the naringinase in enzyme preparation, improve the mouthfeel of fresh fruit raw material, it can by carboxypeptidase To carry out depickling to fresh fruit raw material, the problem of avoiding fresh fruit acid excessive growth spoilage organisms, fresh fruit raw material is further increased Storage period.
The present invention proposes a kind of production technology using lactic acid bacteria and enzyme preparation preservation fruit embryo, comprising the following steps:
Step 1: selection fresh fruit raw material
The fresh fruit raw material for selecting fresh, mature, nothing to addle;
Step 2: cleaning
The light salt brine of fresh fruit raw material and 3% is put into fermentation vat and is carried out immersion 20~30 minutes, it is clear with flowing after immersion Water rinses fresh fruit raw material well;
Step 3: pretreatment
Fresh fruit raw material is carried out using perforator to prick hole processing, fresh fruit raw material is subjected to stoning processing again after pricking hole processing, Fresh fruit raw material is cut in half after stoning, fruit block is made;
Step 4: high temperature, which scalds, to be boiled
The pretreated fresh fruit raw material of above-mentioned steps three and clear water are added according to the ratio of mass ratio 1:3 and scalded in saucepan, It is heated, carries out quickly scalding after heating and boil 10~12 minutes, scalded taking-up fresh fruit raw material after boiling and carry out cooling treatment, until fresh fruit Raw material is cooled to room temperature;
Step 5: rinsing
Fresh fruit raw material after cooling treatment is taken out, is put into salt, citric acid and clear water according to the ratio of mass ratio 5:1:94 It is rinsed in rinsing liquid made of example configuration, rinses fresh fruit raw material with deionized water again after rinsing;
Step 6: configuration lactic acid bacteria soak
Lactic acid bacteria, clear water, salt and glucose are added according to the ratio of mass ratio 1:100:0.1:3~2:160:0.3:5 Enter in reaction vessel and cultivated, lactic acid bacteria soak is made;
Step 7: configuration enzyme preparation
By cellulase, carboxypeptidase, lactase and naringinase according to mass ratio 1:0.6:0.8:0.6~1.2:0.8:1: 0.8 ratio is added in culture bottle and is configured, and enzyme preparation is made;
Step 8: compounding maceration extract
Supersonic generator is added according to the ratio of mass ratio 20:1:5 in lactic acid bacteria soak, enzyme preparation and deionized water It inside carries out mixing 3~5 minutes, maceration extract is made;
Step 9: ultrasonic permeability dipping
Pretreated fresh fruit raw material is added in supersonic generator and is stirred 15~20 minutes, is surpassed after stirring Sound wave infiltration processing 20~25 minutes, it is sealed enzymatic hydrolysis and fermentation after ultrasonic permeability processing, dipping fresh fruit raw material is made, can grow Phase preservation;
Step 10: freeze-day with constant temperature
It is sealed by fermentation heel row and goes out the maceration extract in supersonic generator, dipping fresh fruit raw material taking-up is put into microwave vacuum and is done Freeze-day with constant temperature processing is carried out in dry device, dry extremely dipping fresh fruit moisture content of the raw material is 55%~65%, and fruit embryo is made.
Further improvement lies in that: the mass ratio of light salt brine and fresh fruit raw material is 2:1 in the step 2, in soaking process, Every immersion repeats 4~6 completion immersion after five minutes to carrying out in fermentation vat agitation 1~2 minute.
Further improvement lies in that: the fresh fruit raw material single fruit that hole density is 2.5~3.2 centimetres of diameter is pricked in the step 3 to be pricked Hole count 6~8,3.3~4.5 centimetres of diameter of fresh fruit raw material single fruit pricks hole count 10~12.
Further improvement lies in that: heating temperature is 125~130 degrees Celsius in the step 4, and cooling treatment uses ice water Propose leaching method, cooling treatment detailed process are as follows: the fresh fruit raw material scalded after boiling is pulled out out of boiling hot saucepan, is put into ice water and carries out at once Leaching is proposed, fresh fruit raw material is put into sterile cooler bin after mentioning leaching 5~6 times repeatedly and is cooled to room temperature.
Further improvement lies in that: detailed process in the step 5 are as follows: will be cooled to room temperature fresh fruit raw material be put into salt, Rinsing is carried out 15~20 minutes in rinsing liquid made of the proportional arrangement of citric acid and clear water according to mass ratio 5:1:94, it is rinsed Fresh fruit raw material is stirred in journey, fresh fruit raw material is allowed sufficiently to rinse, rinses fresh fruit raw material with deionized water after rinsing, washes fresh fruit original Expect the rinsing liquid of surface residual.
Further improvement lies in that: detailed process in the step 6 are as follows: lactic acid bacteria and clear water are added in reaction vessel, into Row stirring 3~5 minutes, control mixing speed are 30~40 revs/min, and reaction vessel is added in salt and glucose after stirring In, lactic acid bacteria, clear water, salt and glucose mass ratio be 1:100:0.1:3, controlling temperature in reaction vessel is 26~30 to take the photograph Family name's degree carries out culture 20~22 hours, and lactic acid bacteria soak is made.
Further improvement lies in that: detailed process in the step 7 are as follows: by cellulase, carboxypeptidase, lactase and naringin Enzyme is put into culture bottle according to the ratio of mass ratio 1:0.6:0.8:0.6, and culture bottle is put into constant temperature incubator, controls constant temperature Cultivating the temperature inside the box is 36~37 degrees Celsius, carries out configuration 30~36 hours, enzyme preparation is made.
Further improvement lies in that: detailed process in the step 8 are as follows: first lactic acid bacteria soak and enzyme preparation are added super It carries out mixing ultrasound infiltration 5~10 minutes in sonic generator, then deionized water is added in supersonic generator, lactic acid bacteria leaching Steeping liquid, enzyme preparation and deionized water quality ratio is 20:1:5, be mixed 5 minutes, carries out ultrasonic infiltration after stirring again 10 minutes, maceration extract is made.
Further improvement lies in that: detailed process in the step 9 are as follows: ultrasonic wave is added in pretreated fresh fruit raw material It is stirred in generator 15~20 minutes, control speed of agitator is 100~120 revs/min, and ultrasonic permeability is carried out after stirring Processing 20~25 minutes, it is 28~36 degrees Celsius that temperature in supersonic generator is controlled after ultrasonic permeability processing, and is controlled super Pressure is 0.5~0.8Mpa in sonic generator, is sealed enzymatic hydrolysis and fermentation 30 hours, and dipping fresh fruit raw material is made.
Further improvement lies in that: detailed process in the step 10 are as follows: be sealed by fermentation heel row and go out in supersonic generator Maceration extract, will dipping fresh fruit raw material taking-up be put into microwave vacuum dryer carry out freeze-day with constant temperature handle to dipping fresh fruit raw material contain Water rate is 55%~65%, and controlling temperature in microwave vacuum dryer is 55~65 degrees Celsius, and vacuum degree is 1.3~1.8Mpa, Fruit embryo is made.
The invention has the benefit that can use the special physiological activity of lactic acid bacteria by lactic acid bacteria soak to keep Nutritive value of food not only can be improved in the microecological balance of fruit preservation environment, improves flavour of food products, can also improve food Product keeping quality and added value, can inhibit the generation of raw material preserving process spoilage organisms growth and breeding and spoilage product, while can be with Nutriment is manufactured, the tissue development of fresh fruit raw material is stimulated, is actively made to be generated to nutritional status, the preservation process of fruit body With, de- hardship can be carried out to fresh fruit raw material by the naringinase in enzyme preparation, improve the mouthfeel of fresh fruit raw material, it can by carboxypeptidase To carry out depickling to fresh fruit raw material, the problem of avoiding fresh fruit acid excessive growth spoilage organisms, fresh fruit raw material is further increased Storage period is scalded by high temperature and proposes leaching with ice water after boiling and can carry out color protection to fresh fruit raw material, at the same ice water mention leaching can be improved it is fresh The reservation of beneficiating ingredient in fruit raw material improves the nutritive value of fresh fruit raw material, and being handled by ultrasonic permeability can be improved fresh fruit Absorption to lactic acid bacteria soak and enzyme preparation, permeability is more preferable, and carrying out freeze-day with constant temperature by microwave vacuum dryer can mention High drying efficiency, rate of drying is fast and uniform drying, can effectively shorten the period of entire fruit embryo production, be suitable for high-volume Fruit embryo production.
Specific embodiment
In order to realize invention technological means, reach purpose and effect is easy to understand, below with reference to specific implementation Mode, the present invention is further explained.
Embodiment one
A kind of production technology using lactic acid bacteria and enzyme preparation preservation fruit embryo
Step 1: selection fresh fruit raw material
The Chinese olive for selecting fresh, mature, nothing to addle;
Step 2: cleaning
The light salt brine of Chinese olive and 3% is put into fermentation vat according to the ratio of mass ratio 2:1 and is impregnated, it is every to impregnate 5 points It to agitation 2 minutes is carried out in fermentation vat after clock, is repeated 6 times, carries out impregnating 30 minutes altogether, stir 12 minutes, with stream after immersion Dynamic clear water rinses Chinese olive well;
Step 3: pretreatment
Chinese olive is carried out using perforator to prick hole processing, the Chinese olive single fruit that hole density is 2.5 centimetres of diameter is pricked and pricks hole count 8 A, 3.3 centimetres of diameter of Chinese olive single fruit pricks hole count 12, and Chinese olive is carried out stoning processing again after pricking hole processing, will be green after stoning Fruit is cut in half, and fruit block is made;
Step 4: high temperature, which scalds, to be boiled
The pretreated Chinese olive of above-mentioned steps three and clear water are added according to the ratio of mass ratio 1:3 and scalded in saucepan, is carried out Heating, heating temperature are 130 degrees Celsius, and progress is quickly scalded and boiled 10 minutes after heating, scald taking-up Chinese olive after boiling and ice water is used to propose leaching Method carries out cooling treatment, and the Chinese olive scalded after boiling is pulled out out of boiling hot saucepan, is put into carries out proposing leaching in ice water at once, propose leaching 5 repeatedly Chinese olive is put into sterile cooler bin after secondary and is cooled to room temperature;
Step 5: rinsing
The Chinese olive that will be cooled to room temperature is put into salt, citric acid and clear water and forms according to the proportional arrangement of mass ratio 5:1:94 Rinsing liquid in carry out rinsing 18 minutes, Chinese olive is stirred in rinse cycle, Chinese olive is allowed sufficiently to rinse, is rushed after rinsing with deionized water Chinese olive is washed, the rinsing liquid of Chinese olive surface residual is washed;
Step 6: configuration lactic acid bacteria soak
Lactic acid bacteria and clear water are added in reaction vessel, are stirred 5 minutes, control mixing speed is 30 revs/min, is stirred Salt and glucose are added in reaction vessel after mixing, lactic acid bacteria, clear water, salt and glucose mass ratio be 1:100:0.1: 3, controlling temperature in reaction vessel is 28 degrees Celsius, carries out culture 22 hours, lactic acid bacteria soak is made;
Step 7: configuration enzyme preparation
Cellulase, carboxypeptidase, lactase and naringinase are put into culture according to the ratio of mass ratio 1:0.6:0.8:0.6 In bottle, culture bottle is put into constant temperature incubator, it is 36 degrees Celsius that control constant temperature, which cultivates the temperature inside the box, configuration 33 hours is carried out, Enzyme preparation is made;
Step 8: compounding maceration extract
First lactic acid bacteria soak and enzyme preparation are added in supersonic generator and mix ultrasound infiltration 8 minutes, then will Deionized water is added in supersonic generator, and lactic acid bacteria soak, enzyme preparation and deionized water quality ratio are 20:1:5, is mixed Stirring 5 minutes is closed, ultrasound infiltration 10 minutes is carried out after stirring again, maceration extract is made;
Step 9: ultrasonic permeability dipping
Will pretreated Chinese olive be added supersonic generator in be stirred 20 minutes, control speed of agitator be 100 turns/ Minute, ultrasonic permeability is carried out after stirring and is handled 25 minutes, and temperature in supersonic generator is controlled after ultrasonic permeability processing is 32 degrees Celsius, and controlling pressure in supersonic generator is 0.8Mpa, is sealed enzymatic hydrolysis and fermentation 30 hours, it is green that dipping is made Fruit;
Step 10: freeze-day with constant temperature
It is sealed by fermentation heel row and goes out the maceration extract in supersonic generator, dipping Chinese olive taking-up is put into microwave vacuum dryer Interior progress freeze-day with constant temperature processing, until dipping Chinese olive moisture content is 60%, controlling temperature in microwave vacuum dryer is 60 Celsius Fruit embryo is made in degree, vacuum degree 1.5Mpa.
Embodiment two
A kind of production technology using lactic acid bacteria and enzyme preparation preservation fruit embryo
Step 1: selection fresh fruit raw material
The plum for selecting fresh, mature, nothing to addle;
Step 2: cleaning
The light salt brine of plum and 3% is put into fermentation vat according to the ratio of mass ratio 2:1 and is impregnated, it is every to impregnate 5 points It to agitation 2 minutes is carried out in fermentation vat after clock, is repeated 6 times, carries out impregnating 30 minutes altogether, stir 12 minutes, with stream after immersion Dynamic clear water rinses plum well;
Step 3: pretreatment
Plum is carried out using perforator to prick hole processing, the plum single fruit that hole density is 2.5 centimetres of diameter is pricked and pricks hole count 8 A, 3.3 centimetres of diameter of plum single fruit pricks hole count 12, plum is carried out stoning processing again after pricking hole processing, by Lee after stoning Son is cut in half, and fruit block is made;
Step 4: high temperature, which scalds, to be boiled
The pretreated plum of above-mentioned steps three and clear water are added according to the ratio of mass ratio 1:3 and scalded in saucepan, is carried out Heating, heating temperature are 130 degrees Celsius, and progress is quickly scalded and boiled 10 minutes after heating, scald taking-up plum after boiling and ice water is used to propose leaching Method carries out cooling treatment, and the plum scalded after boiling is pulled out out of boiling hot saucepan, is put into carries out proposing leaching in ice water at once, propose leaching 5 repeatedly Plum is put into sterile cooler bin after secondary and is cooled to room temperature;
Step 5: rinsing
The plum that will be cooled to room temperature is put into salt, citric acid and clear water and forms according to the proportional arrangement of mass ratio 5:1:94 Rinsing liquid in carry out rinsing 18 minutes, plum is stirred in rinse cycle, plum is allowed sufficiently to rinse, is rushed after rinsing with deionized water Plum is washed, the rinsing liquid of plum surface residual is washed;
Step 6: configuration lactic acid bacteria soak
Lactic acid bacteria and clear water are added in reaction vessel, are stirred 5 minutes, control mixing speed is 30 revs/min, is stirred Salt and glucose are added in reaction vessel after mixing, lactic acid bacteria, clear water, salt and glucose mass ratio be 1:100:0.1: 3, controlling temperature in reaction vessel is 28 degrees Celsius, carries out culture 22 hours, lactic acid bacteria soak is made;
Step 7: configuration enzyme preparation
Cellulase, carboxypeptidase, lactase and naringinase are put into culture according to the ratio of mass ratio 1:0.6:0.8:0.6 In bottle, culture bottle is put into constant temperature incubator, it is 36 degrees Celsius that control constant temperature, which cultivates the temperature inside the box, configuration 33 hours is carried out, Enzyme preparation is made;
Step 8: compounding maceration extract
First lactic acid bacteria soak and enzyme preparation are added in supersonic generator and mix ultrasound infiltration 8 minutes, then will Deionized water is added in supersonic generator, and lactic acid bacteria soak, enzyme preparation and deionized water quality ratio are 20:1:5, is mixed Stirring 5 minutes is closed, ultrasound infiltration 10 minutes is carried out after stirring again, maceration extract is made;
Step 9: ultrasonic permeability dipping
Will pretreated plum be added supersonic generator in be stirred 20 minutes, control speed of agitator be 100 turns/ Minute, ultrasonic permeability is carried out after stirring and is handled 25 minutes, and temperature in supersonic generator is controlled after ultrasonic permeability processing is 32 degrees Celsius, and controlling pressure in supersonic generator is 0.8Mpa, is sealed enzymatic hydrolysis and fermentation 30 hours, and dipping Lee is made Son;
Step 10: freeze-day with constant temperature
It is sealed by fermentation heel row and goes out the maceration extract in supersonic generator, dipping plum taking-up is put into microwave vacuum dryer Interior progress freeze-day with constant temperature processing, until dipping plum moisture content is 60%, controlling temperature in microwave vacuum dryer is 60 Celsius Fruit embryo is made in degree, vacuum degree 1.5Mpa.
Embodiment three
A kind of production technology using lactic acid bacteria and enzyme preparation preservation fruit embryo
Step 1: selection fresh fruit raw material
The red bayberry for selecting fresh, mature, nothing to addle;
Step 2: cleaning
The light salt brine of red bayberry and 3% is put into fermentation vat according to the ratio of mass ratio 2:1 and is impregnated, it is every to impregnate 5 points It to agitation 2 minutes is carried out in fermentation vat after clock, is repeated 6 times, carries out impregnating 30 minutes altogether, stir 12 minutes, with stream after immersion Dynamic clear water rinses red bayberry well;
Step 3: pretreatment
Red bayberry is carried out using perforator to prick hole processing, the red bayberry single fruit that hole density is 2.5 centimetres of diameter is pricked and pricks hole count 8 A, 3.3 centimetres of diameter of red bayberry single fruit pricks hole count 12, and fruit block is made;
Step 4: high temperature, which scalds, to be boiled
The pretreated red bayberry of above-mentioned steps three and clear water are added according to the ratio of mass ratio 1:3 and scalded in saucepan, is carried out Heating, heating temperature are 130 degrees Celsius, and progress is quickly scalded and boiled 10 minutes after heating, scald taking-up red bayberry after boiling and ice water is used to propose leaching Method carries out cooling treatment, and the red bayberry scalded after boiling is pulled out out of boiling hot saucepan, is put into carries out proposing leaching in ice water at once, propose leaching 5 repeatedly Red bayberry is put into sterile cooler bin after secondary and is cooled to room temperature;
Step 5: rinsing
The red bayberry that will be cooled to room temperature is put into salt, citric acid and clear water and forms according to the proportional arrangement of mass ratio 5:1:94 Rinsing liquid in carry out rinsing 18 minutes, red bayberry is stirred in rinse cycle, red bayberry is allowed sufficiently to rinse, is rushed after rinsing with deionized water Red bayberry is washed, the rinsing liquid of red bayberry surface residual is washed;
Step 6: configuration lactic acid bacteria soak
Lactic acid bacteria and clear water are added in reaction vessel, are stirred 5 minutes, control mixing speed is 30 revs/min, is stirred Salt and glucose are added in reaction vessel after mixing, lactic acid bacteria, clear water, salt and glucose mass ratio be 1:100:0.1: 3, control reaction vessel medium temperature
Degree is 28 degrees Celsius, carries out culture 22 hours, lactic acid bacteria soak is made;
Step 7: configuration enzyme preparation
Cellulase, carboxypeptidase, lactase and naringinase are put into culture according to the ratio of mass ratio 1:0.6:0.8:0.6 In bottle, culture bottle is put into constant temperature incubator, it is 36 degrees Celsius that control constant temperature, which cultivates the temperature inside the box, configuration 33 hours is carried out, Enzyme preparation is made;
Step 8: compounding maceration extract
First lactic acid bacteria soak and enzyme preparation are added in supersonic generator and mix ultrasound infiltration 8 minutes, then will Deionized water is added in supersonic generator, and lactic acid bacteria soak, enzyme preparation and deionized water quality ratio are 20:1:5, is mixed Stirring 5 minutes is closed, ultrasound infiltration 10 minutes is carried out after stirring again, maceration extract is made;
Step 9: ultrasonic permeability dipping
Will pretreated red bayberry be added supersonic generator in be stirred 20 minutes, control speed of agitator be 100 turns/ Minute, ultrasonic permeability is carried out after stirring and is handled 25 minutes, and temperature in supersonic generator is controlled after ultrasonic permeability processing is 32 degrees Celsius, and controlling pressure in supersonic generator is 0.8Mpa, is sealed enzymatic hydrolysis and fermentation 30 hours, and dipping poplar is made Plum;
Step 10: freeze-day with constant temperature
It is sealed by fermentation heel row and goes out the maceration extract in supersonic generator, dipping red bayberry is taken out and is put into microwave vacuum dryer Interior progress freeze-day with constant temperature processing, until dipping red bayberry moisture content is 60%, controlling temperature in microwave vacuum dryer is 60 Celsius Fruit embryo is made in degree, vacuum degree 1.5Mpa.
According to embodiment one, embodiment two and embodiment three, it can be concluded that, the method for the present invention is suitable for Chinese olive, plum and poplar The fruit embryo preservation of the fruit such as plum, the microecological balance of fruit preservation environment is kept by the special physiological activity of lactic acid bacteria, no Only can be improved nutritive value of food, improve flavour of food products, can also inhibit raw material preserving process spoilage organisms growth and breeding and The generation of spoilage product, naringinase can carry out de- hardship to fresh fruit raw material, improve the mouthfeel of fresh fruit raw material, can be with by carboxypeptidase The problem of depickling is carried out to fresh fruit raw material, avoids fresh fruit acidity excessive growth spoilage organisms.
It can use the special physiological activity of lactic acid bacteria by lactic acid bacteria soak to keep micro- life of fruit preservation environment State balance, not only can be improved nutritive value of food, improves flavour of food products, can also improve food preservation and added value, It can inhibit the generation of raw material preserving process spoilage organisms growth and breeding and spoilage product, while nutriment can be manufactured, stimulate The tissue development of fresh fruit raw material passes through the shaddock in enzyme preparation so that nutritional status, the preservation process to fruit body generate positive effect Glycosides enzyme can carry out de- hardship to fresh fruit raw material, improve the mouthfeel of fresh fruit raw material, can be taken off to fresh fruit raw material by carboxypeptidase Acid, further increases the Storage period of fresh fruit raw material, boils by the way that high temperature is boiling hot the problem of avoiding fresh fruit acid excessive growths spoilage organisms Color protection can be carried out to fresh fruit raw material by proposing leaching with ice water afterwards, while ice water mentions the guarantor for soaking and beneficiating ingredient in fresh fruit raw material can be improved It stays, improves the nutritive value of fresh fruit raw material, being handled by ultrasonic permeability can be improved fresh fruit to lactic acid bacteria soak and enzyme system The absorption of agent, permeability is more preferable, and carrying out freeze-day with constant temperature by microwave vacuum dryer can be improved drying efficiency, and rate of drying is fast And uniform drying, it can effectively shorten the period of entire fruit embryo production, be suitable for large batch of fruit embryo and produce.
The basic principles, main features and advantages of the invention have been shown and described above.The technical staff of the industry should Understand, the present invention is not limited to the above embodiments, and the above embodiments and description only describe originals of the invention Reason, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these changes and improvements It all fall within the protetion scope of the claimed invention.The claimed scope of the invention is by appended claims and its equivalent circle It is fixed.

Claims (10)

1. a kind of production technology using lactic acid bacteria and enzyme preparation preservation fruit embryo, it is characterised in that: the following steps are included:
Step 1: selection fresh fruit raw material
The fresh fruit raw material for selecting fresh, mature, nothing to addle;
Step 2: cleaning
The light salt brine of fresh fruit raw material and 3% is put into fermentation vat and is carried out immersion 20~30 minutes, it will with flowing clear water after immersion Fresh fruit raw material is rinsed well;
Step 3: pretreatment
Fresh fruit raw material is carried out using perforator to prick hole processing, fresh fruit raw material is subjected to stoning processing again after pricking hole processing, is enucleated Fresh fruit raw material is cut in half afterwards, fruit block is made;
Step 4: high temperature, which scalds, to be boiled
The pretreated fresh fruit raw material of above-mentioned steps three and clear water are added according to the ratio of mass ratio 1:3 and scalded in saucepan, is carried out Heating, progress is quickly scalded and is boiled 10~12 minutes after heating, is scalded and is taken out fresh fruit raw material progress cooling treatment after boiling, until fresh fruit raw material It is cooled to room temperature;
Step 5: rinsing
Fresh fruit raw material after cooling treatment is taken out, salt, citric acid and clear water is put into and matches according to the ratio of mass ratio 5:1:94 It is rinsed in rinsing liquid made of setting, rinses fresh fruit raw material with deionized water again after rinsing;
Step 6: configuration lactic acid bacteria soak
Lactic acid bacteria, clear water, salt and glucose are added instead according to the ratio of mass ratio 1:100:0.1:3~2:160:0.3:5 It answers in container and is cultivated, lactic acid bacteria soak is made;
Step 7: configuration enzyme preparation
By cellulase, carboxypeptidase, lactase and naringinase according to mass ratio 1:0.6:0.8:0.6~1.2:0.8:1:0.8's Ratio is added in culture bottle and is configured, and enzyme preparation is made;
Step 8: compounding maceration extract
By lactic acid bacteria soak, enzyme preparation and deionized water according to mass ratio 20:1:5 ratio be added supersonic generator in into Row is mixed 3~5 minutes, and maceration extract is made;
Step 9: ultrasonic permeability dipping
Pretreated fresh fruit raw material is added in supersonic generator and is stirred 15~20 minutes, ultrasonic wave is carried out after stirring Infiltration processing 20~25 minutes is sealed enzymatic hydrolysis and fermentation after ultrasonic permeability processing, and dipping fresh fruit raw material is made;
Step 10: freeze-day with constant temperature
It is sealed by fermentation heel row and goes out the maceration extract in supersonic generator, dipping fresh fruit raw material taking-up is put into microwave vacuum dryer Interior progress freeze-day with constant temperature processing, dry extremely dipping fresh fruit moisture content of the raw material is 55%~65%, and fruit embryo is made.
2. a kind of production technology using lactic acid bacteria and enzyme preparation preservation fruit embryo according to claim 1, it is characterised in that: The mass ratio of light salt brine and fresh fruit raw material is 2:1 in the step 2, and in soaking process, every immersion is after five minutes in fermentation vat It carries out agitation 1~2 minute, repeats 4~6 completions and impregnate.
3. a kind of production technology using lactic acid bacteria and enzyme preparation preservation fruit embryo according to claim 1, it is characterised in that: The fresh fruit raw material single fruit that hole density is 2.5~3.2 centimetres of diameter is pricked in the step 3 pricks hole count 6~8, diameter 3.3~4.5 Centimetre fresh fruit raw material single fruit prick hole count 10~12.
4. a kind of production technology using lactic acid bacteria and enzyme preparation preservation fruit embryo according to claim 1, it is characterised in that: Heating temperature is 125~130 degrees Celsius in the step 4, and cooling treatment proposes leaching method, cooling treatment detailed process using ice water Are as follows: the fresh fruit raw material scalded after boiling is pulled out out of boiling hot saucepan, is put into carries out proposing leaching in ice water at once, it will after mentioning leaching 5~6 times repeatedly Fresh fruit raw material is put into sterile cooler bin and is cooled to room temperature.
5. a kind of production technology using lactic acid bacteria and enzyme preparation preservation fruit embryo according to claim 1, it is characterised in that: Detailed process in the step 5 are as follows: the fresh fruit raw material that will be cooled to room temperature is put into salt, citric acid and clear water according to mass ratio It is carried out in rinsing liquid made of the proportional arrangement of 5:1:94 rinsing 15~20 minutes, fresh fruit raw material is stirred in rinse cycle, is allowed fresh Fruit raw material sufficiently rinses, and rinses fresh fruit raw material with deionized water after rinsing, washes the rinsing liquid of fresh fruit raw material surface residual.
6. a kind of production technology using lactic acid bacteria and enzyme preparation preservation fruit embryo according to claim 1, it is characterised in that: Detailed process in the step 6 are as follows: lactic acid bacteria and clear water are added in reaction vessel, are stirred 3~5 minutes, control stirring Speed is 30~40 revs/min, salt and glucose is added in reaction vessel after stirring, lactic acid bacteria, clear water, salt and grape The mass ratio of sugar is 1:100:0.1:3, and controlling temperature in reaction vessel is 26~30 degrees Celsius, carries out culture 20~22 hours, Lactic acid bacteria soak is made.
7. a kind of production technology using lactic acid bacteria and enzyme preparation preservation fruit embryo according to claim 1, it is characterised in that: Detailed process in the step 7 are as follows: by cellulase, carboxypeptidase, lactase and naringinase according to mass ratio 1:0.6:0.8: 0.6 ratio is put into culture bottle, and culture bottle is put into constant temperature incubator, and control constant temperature is cultivated the temperature inside the box and taken the photograph for 36~37 Family name's degree carries out configuration 30~36 hours, enzyme preparation is made.
8. a kind of production technology using lactic acid bacteria and enzyme preparation preservation fruit embryo according to claim 1, it is characterised in that: Detailed process in the step 8 are as follows: first lactic acid bacteria soak and enzyme preparation are added in supersonic generator and carry out mixing ultrasound Infiltration 5~10 minutes, then deionized water is added in supersonic generator, lactic acid bacteria soak, enzyme preparation and deionized water Amount be mixed 5 minutes than being 20:1:5, carries out ultrasound infiltration 10 minutes after stirring again, maceration extract is made.
9. a kind of production technology using lactic acid bacteria and enzyme preparation preservation fruit embryo according to claim 1, it is characterised in that: Detailed process in the step 9 are as follows: pretreated fresh fruit raw material is added in supersonic generator and is stirred 15~20 points Clock, control speed of agitator are 100~120 revs/min, and ultrasonic permeability is carried out after stirring and is handled 20~25 minutes, ultrasonic wave seeps Controlling temperature in supersonic generator after processing thoroughly is 28~36 degrees Celsius, and control in supersonic generator pressure be 0.5~ 0.8Mpa is sealed enzymatic hydrolysis and fermentation 30 hours, and dipping fresh fruit raw material is made.
10. a kind of production technology using lactic acid bacteria and enzyme preparation preservation fruit embryo according to claim 1, feature exist In: detailed process in the step 10 are as follows: be sealed by fermentation heel row and go out the maceration extract in supersonic generator, fresh fruit raw material will be impregnated Taking-up, which is put into microwave vacuum dryer, to be carried out freeze-day with constant temperature to handle to dipping fresh fruit moisture content of the raw material being 55%~65%, control Temperature is 55~65 degrees Celsius in microwave vacuum dryer, and vacuum degree is 1.3~1.8Mpa, and fruit embryo is made.
CN201811257476.8A 2018-10-26 2018-10-26 A kind of production technology using lactic acid bacteria and enzyme preparation preservation fruit embryo Pending CN109329543A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201811257476.8A CN109329543A (en) 2018-10-26 2018-10-26 A kind of production technology using lactic acid bacteria and enzyme preparation preservation fruit embryo

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201811257476.8A CN109329543A (en) 2018-10-26 2018-10-26 A kind of production technology using lactic acid bacteria and enzyme preparation preservation fruit embryo

Publications (1)

Publication Number Publication Date
CN109329543A true CN109329543A (en) 2019-02-15

Family

ID=65311921

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201811257476.8A Pending CN109329543A (en) 2018-10-26 2018-10-26 A kind of production technology using lactic acid bacteria and enzyme preparation preservation fruit embryo

Country Status (1)

Country Link
CN (1) CN109329543A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114568662A (en) * 2022-03-19 2022-06-03 佳木斯沐禾生物科技有限公司 Whole-fruit can of pulp fruits in cold region and processing technology of whole-fruit can

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2358730A1 (en) * 2009-10-15 2011-08-24 Purecircle SDN BHD High-purity rebaudioside d and applications
CN102630796A (en) * 2012-05-10 2012-08-15 陈鹏 Technology for processing glace fruit through biological method
CN103609819A (en) * 2013-12-06 2014-03-05 广西壮族自治区农业科学院农产品加工研究所 Low-sugar preserved mango and preparation method thereof
CN103652252A (en) * 2013-12-03 2014-03-26 云南大理洱宝实业有限公司 Flower plum and preparation method thereof
CN105076648A (en) * 2015-09-02 2015-11-25 付宏存 Blueberry tablet candy with beautifying and antibacterial efficacy and preparation method thereof
CN105230922A (en) * 2015-09-29 2016-01-13 明光市农源农作物专业合作社 Green tea-flavored sugar-free preserved kiwi fruit and preparation method thereof
CN106072104A (en) * 2016-06-07 2016-11-09 江苏省农业科学院 A kind of rich probiotic bacteria Fructus Mali pumilae crisp product and preparation method thereof
KR101753661B1 (en) * 2015-12-08 2017-07-05 샘표식품 주식회사 Method for improving flavor of ginseng
CN108576345A (en) * 2018-03-27 2018-09-28 靖西市秀美边城农业科技有限公司 A kind of pawpaw flavor low sugar shaddock ped preserved fruit and preparation method thereof

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2358730A1 (en) * 2009-10-15 2011-08-24 Purecircle SDN BHD High-purity rebaudioside d and applications
CN102630796A (en) * 2012-05-10 2012-08-15 陈鹏 Technology for processing glace fruit through biological method
CN103652252A (en) * 2013-12-03 2014-03-26 云南大理洱宝实业有限公司 Flower plum and preparation method thereof
CN103609819A (en) * 2013-12-06 2014-03-05 广西壮族自治区农业科学院农产品加工研究所 Low-sugar preserved mango and preparation method thereof
CN105076648A (en) * 2015-09-02 2015-11-25 付宏存 Blueberry tablet candy with beautifying and antibacterial efficacy and preparation method thereof
CN105230922A (en) * 2015-09-29 2016-01-13 明光市农源农作物专业合作社 Green tea-flavored sugar-free preserved kiwi fruit and preparation method thereof
KR101753661B1 (en) * 2015-12-08 2017-07-05 샘표식품 주식회사 Method for improving flavor of ginseng
CN106072104A (en) * 2016-06-07 2016-11-09 江苏省农业科学院 A kind of rich probiotic bacteria Fructus Mali pumilae crisp product and preparation method thereof
CN108576345A (en) * 2018-03-27 2018-09-28 靖西市秀美边城农业科技有限公司 A kind of pawpaw flavor low sugar shaddock ped preserved fruit and preparation method thereof

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
崔莉等: ""胡萝卜片中富集植物乳杆菌的工艺优化"", 《食品科学》 *
李媛等: ""基于乳酸菌发酵法的果坯保藏工艺"", 《食品工业科技》 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114568662A (en) * 2022-03-19 2022-06-03 佳木斯沐禾生物科技有限公司 Whole-fruit can of pulp fruits in cold region and processing technology of whole-fruit can

Similar Documents

Publication Publication Date Title
CN102342322B (en) Purple potato/corn particle lactobacillus milk beverage and preparation method thereof
CN105248746A (en) Lemon loquat flower tea capable of strengthening stomach and preparation method thereof
CN103445221B (en) Dried salted marine eel treatment process
CN102524618A (en) Method for making mango paste by using peel and flesh of mango
CN106213086A (en) A kind of composite probiotics ferment yellow peach clear-juice beverage and preparation method thereof
CN103535780B (en) Method for preparing instant soft-shell shrimps
CN109329543A (en) A kind of production technology using lactic acid bacteria and enzyme preparation preservation fruit embryo
CN100361588C (en) Method for preparing walnut sour milk
CN107129899A (en) Puerpera is with the preparation method and puerpera's fermented glutinous rice of fermented glutinous rice
CN106690164A (en) Preparation method of canned yellow peaches
CN107198161A (en) A kind of preparation method of dried radish
CN101690516A (en) Preparation method of Nostoc commune sour milk
CN109497446A (en) Using the meat products production technology of complex enzyme formulation
CN109924376A (en) A kind of Kiwi berry NFC fruit juice and preparation method thereof
CN106616619A (en) Preparation method of canned orange
CN106509733A (en) Preparation method of canned red bayberry fruits
CN103005519A (en) Salting process of freshwater fish
CN106666046A (en) Preserved kiwifruits and manufacture method thereof
CN105248741A (en) Bamboo leaf loquat flower tea capable of clearing heat, and preparation method thereof
CN110012931A (en) A kind of combination dehydration preparation method of Tomato Dry
RU2715332C1 (en) Smoked calmar production method
CN107549429A (en) A kind of preserved apricot processing method
CN106819950A (en) The preparation method of potato leaf sea cucumber soup
CN107410920A (en) A kind of processing method of the sandwich sauce of three delicacies flavor
CN106616865A (en) Preparation method of tomato meat paste

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication

Application publication date: 20190215

RJ01 Rejection of invention patent application after publication