CN109323908A - A kind of detection method for mass spectrum streaming technology - Google Patents
A kind of detection method for mass spectrum streaming technology Download PDFInfo
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- CN109323908A CN109323908A CN201811180002.8A CN201811180002A CN109323908A CN 109323908 A CN109323908 A CN 109323908A CN 201811180002 A CN201811180002 A CN 201811180002A CN 109323908 A CN109323908 A CN 109323908A
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- 238000001819 mass spectrum Methods 0.000 title claims abstract description 22
- 238000001514 detection method Methods 0.000 title claims abstract description 20
- 238000005516 engineering process Methods 0.000 title claims abstract description 14
- 238000004043 dyeing Methods 0.000 claims abstract description 18
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 11
- 230000003834 intracellular effect Effects 0.000 claims abstract description 9
- 230000000149 penetrating effect Effects 0.000 claims abstract description 9
- 239000003550 marker Substances 0.000 claims abstract description 8
- 210000004027 cell Anatomy 0.000 claims description 82
- 239000006228 supernatant Substances 0.000 claims description 30
- 238000005406 washing Methods 0.000 claims description 27
- 239000007788 liquid Substances 0.000 claims description 13
- 239000000203 mixture Substances 0.000 claims description 13
- 238000005119 centrifugation Methods 0.000 claims description 7
- 229910052751 metal Inorganic materials 0.000 claims description 6
- 239000002184 metal Substances 0.000 claims description 6
- 238000011084 recovery Methods 0.000 claims description 6
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 claims description 4
- 101710160107 Outer membrane protein A Proteins 0.000 claims description 3
- 239000000427 antigen Substances 0.000 claims description 3
- 102000036639 antigens Human genes 0.000 claims description 3
- 108091007433 antigens Proteins 0.000 claims description 3
- 238000005138 cryopreservation Methods 0.000 claims description 3
- 239000012153 distilled water Substances 0.000 claims description 3
- 238000007710 freezing Methods 0.000 claims description 3
- 230000008014 freezing Effects 0.000 claims description 3
- 239000011550 stock solution Substances 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 241000143437 Aciculosporium take Species 0.000 claims description 2
- 241000699800 Cricetinae Species 0.000 claims description 2
- 238000009472 formulation Methods 0.000 claims description 2
- 238000000034 method Methods 0.000 abstract description 22
- 239000000523 sample Substances 0.000 abstract description 10
- 239000000975 dye Substances 0.000 abstract description 8
- 238000002474 experimental method Methods 0.000 abstract description 4
- 230000004907 flux Effects 0.000 abstract description 3
- 239000012472 biological sample Substances 0.000 abstract description 2
- 238000007796 conventional method Methods 0.000 abstract description 2
- 238000011527 multiparameter analysis Methods 0.000 abstract description 2
- 210000003819 peripheral blood mononuclear cell Anatomy 0.000 description 7
- 238000004458 analytical method Methods 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 102100024222 B-lymphocyte antigen CD19 Human genes 0.000 description 2
- 101000980825 Homo sapiens B-lymphocyte antigen CD19 Proteins 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 229920001917 Ficoll Polymers 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 239000003146 anticoagulant agent Substances 0.000 description 1
- 229940127219 anticoagulant drug Drugs 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 238000000432 density-gradient centrifugation Methods 0.000 description 1
- 238000007877 drug screening Methods 0.000 description 1
- 238000000684 flow cytometry Methods 0.000 description 1
- 229920000669 heparin Polymers 0.000 description 1
- ZFGMDIBRIDKWMY-PASTXAENSA-N heparin Chemical compound CC(O)=N[C@@H]1[C@@H](O)[C@H](O)[C@@H](COS(O)(=O)=O)O[C@@H]1O[C@@H]1[C@@H](C(O)=O)O[C@@H](O[C@H]2[C@@H]([C@@H](OS(O)(=O)=O)[C@@H](O[C@@H]3[C@@H](OC(O)[C@H](OS(O)(=O)=O)[C@H]3O)C(O)=O)O[C@@H]2O)CS(O)(=O)=O)[C@H](O)[C@H]1O ZFGMDIBRIDKWMY-PASTXAENSA-N 0.000 description 1
- 229960001008 heparin sodium Drugs 0.000 description 1
- 238000013394 immunophenotyping Methods 0.000 description 1
- 238000012083 mass cytometry Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000005259 peripheral blood Anatomy 0.000 description 1
- 239000011886 peripheral blood Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a kind of detection method for mass spectrum streaming technology, mainly includes fixation, Barcode, closing, extracellular dyeing, penetrating, intracellular dyes.Big with flux, accuracy is high, easy to operate, saves reagent, and reduce the error of human factor in experimentation.By fixing to cell, the key technologies details such as Barcode is preferably limited, and establishes the techniqueflow for stablizing specification.This method, which substantially increases sample single experiment, can detecte the quantity of marker, conventional method needs the dying operation that could repeatedly complete, different Barcode samples are mixed now, it is disposable to complete dyeing, efficiently to unicellular carry out multi parameter analysis, with least cell number, the maximum data volume of output.While improving amounts of specimen information, precious biological sample had not only been saved, but also has eliminated and makes a variation between pipe, and simplify workflow, has substantially shortened the manual operation time.
Description
Technical field
The present invention relates to biomedicine fields, and in particular to a kind of detection method for mass spectrum streaming technology.
Background technique
Mass spectrum streaming technology (Mass Cytometry) is integrated with the principle of mass spectrum and flow cytometer, realizes to slender
Born of the same parents carry out multi-parameter detection.It inherits the characteristics of high speed analysis of conventional flow cytometer, and the high score with Mass Spectrometer Method
It distinguishes the technical advantages such as ability and enriched data processing, is a kind of novel single cell analysis technology that developed recently gets up.Matter
Spectrum cell instrument compared with conventional fluorescent measured by flow cytometry parameter more than and channel between do not interfere with, and do not need carry out fluorescence
Compensation calculation has substantially expanded the data quantum of output and detection range of single sample, has made data more comprehensively, as a result more reliable, has become
For a new direction of single cell analysis.
Mass spectrum streaming technology can be used to carry out cell subsets fine immunophenotyping, analyze intracellular signal comprehensively
Access can be carried out the detection of high flux multiparameter to sample, at present in blood, stem cell, immune, cancer and drug screening etc.
Field has a wide range of applications.
Summary of the invention
It is an object of that present invention to provide a kind of detection methods for mass spectrum streaming technology.The detection method is different
The mixing with cells of Barcode coding carries out antibody dyeing together, has good dyeing effect, and method is easy, economical, real
With property is strong, conventional efficient and reliability are higher.
For achieving the above object, the technical solution adopted by the present invention is that:
It is a kind of that for mass spectrum streaming technology, detection method includes the following steps:
A. take cell to be detected into EP pipe, centrifuge washing cell abandons supernatant；
B. it distinguishes cell anyway: dye life or death reagent is added, 4 DEG C are incubated for 5 minutes；
C. it washs: streaming buffer is added, cell is resuspended, centrifuge washing cell abandons supernatant；
D. it fixes: fixer is added, cell is resuspended, be incubated at room temperature 10 minutes；
E. it washs: streaming buffer is added, cell is resuspended, centrifuge washing cell abandons supernatant；
F. cell is resuspended with frozen stock solution, is transferred in cryopreservation tube, is put into gradient freezing storing box, -80 DEG C of refrigerators save；
G. recovery cell: from -80 DEG C of refrigerators take out after recovery cell；
H. cell is transferred in EP pipe, streaming buffer by centrifugation washs cell, abandons supernatant；
I.Barcode: configuration Barcode mix, it is thin that a kind of Barcoding mix resuspension is added in the sample of each EP pipe
Born of the same parents, added Barcoding mix is different in each sample；
J. wash: after streaming buffer resuspension cell is added, centrifuge washing cell abandons supernatant；
K. after different samples being mixed, centrifuge washing cell abandons supernatant；
L. close: after confining liquid is added, 4 DEG C are incubated for 20 minutes；
M. extracellular dyeing: being added the metal marker antibody of cell surface antigen, and 4 DEG C are incubated for 30 minutes；
N. it washs: streaming buffer is added, centrifuge washing cell abandons supernatant；
O. fixating reagent is configured, fixed dye DNA is put into 4 DEG C of refrigerator overnights；
P. it washs: penetrating liquid is added, centrifuge washing cell abandons supernatant；
Q. it is resuspended: Foxp3buffer is added, cell is resuspended, be incubated at room temperature 20 minutes；
R. it washs: penetrating liquid is added, centrifuge washing cell abandons supernatant；
S. dyeing intracellular: being added the metal marker antibody of antigen intracellular, and 4 DEG C are incubated for 30 minutes；
T. it washs: streaming buffer is added, centrifuge washing cell abandons supernatant；
U. machine on: sample is put into mass spectrum cell instrument after being washed with distilled water and is detected and is analyzed.
In above-mentioned technical proposal, it is respectively 0.5%BSA and 0.02% that the streaming buffer formulation, which is containing mass concentration,
1 × PBS of Sodium azide.
In step a, c, the centrifugal rotational speed is 400g, and centrifugation time is 5 minutes；Step e, h, j, k, n, p, r, t
In, the centrifugal rotational speed is 800g, and centrifugation time is 5 minutes.
Barcode mix is selected from 104Pd/105Pd, 104Pd/106Pd, 104Pd/108Pd, 104Pd/ in the step i
110Pd,105Pd/106Pd,105Pd/108Pd,105Pd/110Pd,106Pd/108Pd,106Pd/110Pd,108Pd/
110Pd。
The step l, confining liquid contain Human IgG, Mouse IgG, Hamster IgG and Rat IgG.
Compared with prior art, the device have the advantages that are as follows: the detection of mass spectrum streaming technology provided by the present application
Method, antibody dyeing is handled again by first carrying out cell after fixing process, and will be different sample mix after carry out simultaneously
, it can be achieved that detection flux is big, accuracy is high for antibody dyeing, and human factor influence is smaller.With following innovative point:
1) compared with conventional mass spectrum flow cytometer detection method, less cell number is needed, and is effectively reduced due to even
The loss of cell in continuous operating process；
2) a antibody can dye several groups of samples simultaneously, and the usage amount of antibody is greatly reduced, and reduce experimental cost；
3) experimental differences caused by different time Vertex Coloring are effectively prevented.
To sum up, by fixing to cell, the key technologies details such as Barcode is preferably limited the present invention, is established steady
The techniqueflow of set pattern model.This method, which substantially increases sample single experiment, can detecte the quantity of marker, and conventional method needs
The dying operation could repeatedly complete, mixes different Barcode samples now, disposable to complete dyeing, efficiently
To unicellular carry out multi parameter analysis, with least cell number, the maximum data volume of output.Improving the same of amounts of specimen information
When, precious biological sample was not only saved, but also eliminate and make a variation between pipe, and simplify workflow, and had substantially shortened behaviour manually
Make the time.
Therefore, the method for the present invention has a good application prospect and promotional value, has substantive distinguishing features outstanding and aobvious
The progress of work.
Detailed description of the invention
To make the objectives, technical solutions, and advantages of the present invention clearer, below in conjunction with attached drawing to the present invention into one
The detailed description of step:
Fig. 1 is using the method for the present invention Barcode analysis chart；
Fig. 2 is the mass spectrum streaming figure using the PBMC cell of the method for the present invention dyeing；
Fig. 3 is the mass spectrum streaming figure using the PBMC cell of Fluidigm company method dyeing.
Specific embodiment
Present invention will be further explained below with reference to the attached drawings and examples, it will be appreciated by those skilled in the art that
The following example is merely to illustrate the present invention, and is not construed as limiting the scope of the invention.Actual conditions are not specified in embodiment
Person carries out according to conventional conditions or manufacturer's recommended conditions.Reagents or instruments used without specified manufacturer, being can be with
The conventional products obtained by commercially available purchase.
The present embodiment carries out CD3, Ki67 to the PBMC cell of people using the detection method of mass spectrum streaming technology of the invention
It dyes with CD19, is compared using mass spectrum flow cytomery, and with Fluidigm company method.
Experimental subjects: the peripheral blood of volunteer
Experiment reagent: fixer, fixed/penetrating liquid, penetrating liquid, Barcode reagent, fixating reagent are Fluidigm public affairs
Take charge of product.FoxP3buffer is eBioscience Products.
Experimental facilities: mass spectrum streaming instrument is Fluidigm Products.
Experimental method the following steps are included:
A.EDTA or heparin sodium anticoagulant tube venous whole, Ficoll density-gradient centrifugation method separating peripheral blood mononuclear cells
(Peripheral Blood Mononuclear Cell, PBMC), and count, take suitable cell into EP pipe, centrifuge washing
Cell abandons supernatant；
B. it distinguishes cell anyway: dye life or death reagent is added, 4 DEG C are incubated for 5 minutes；
C. it washs: streaming buffer is added, cell is resuspended, centrifuge washing cell abandons supernatant；
D. it fixes: fixer is added, cell is resuspended, be incubated at room temperature 10 minutes；
E. it washs: streaming buffer is added, cell is resuspended, centrifuge washing cell abandons supernatant；
F. cell is resuspended with frozen stock solution, is transferred in cryopreservation tube, is put into gradient freezing storing box, -80 DEG C of refrigerators save；
G. recovery cell: from -80 DEG C of refrigerators take out after recovery cell；
H. cell is transferred in EP pipe, streaming buffer by centrifugation washs cell, abandons supernatant；
I.Barcode: configuration Barcode mix, a kind of Barcoding mix resuspension cell is added in each sample；
J. wash: after streaming buffer resuspension cell is added, centrifuge washing cell abandons supernatant；
K. after different samples being mixed, centrifuge washing cell abandons supernatant；
L. close: after confining liquid is added, 4 DEG C are incubated for 20 minutes；
M. extracellular dyeing: metal marker the antibody CD3 and CD19 of cell surface antigen is added, 4 DEG C are incubated for 30 minutes；
N. it washs: streaming buffer is added, centrifuge washing cell abandons supernatant；
O. fixating reagent is configured, fixed dye DNA is put into 4 DEG C of refrigerator overnights；
P. it washs: penetrating liquid is added, centrifuge washing cell abandons supernatant；
Q. it is resuspended: Foxp3buffer is added, cell is resuspended, be incubated at room temperature 20 minutes；
R. it washs: penetrating liquid is added, centrifuge washing cell abandons supernatant；
S. dyeing intracellular: the metal marker antibody Ki67 of antigen intracellular is added, 4 DEG C are incubated for 30 minutes；
T. it washs: streaming buffer is added, centrifuge washing cell abandons supernatant；
U. machine on: sample is put into mass spectrum cell instrument after being washed with distilled water and is detected and is analyzed.
Experimental result: the source of different samples, Fig. 1 can effectively be distinguished by Barcode using the method for the present invention
Shown, Patient A, B, C, D represent the cell colony of four patients, and this method and Fluidigm company method are (i.e. conventional
Mass spectrum cell instrument detection method) the mass spectrum fluidic cell figure of PBMC of dyeing is shown in Fig. 2, Fig. 3 respectively, as shown, two methods
Testing result is almost the same.
Table 1 is using the method for the present invention compared with the testing result that Fluidigm company method dyes
Conclusion: the method for the present invention encodes the cell of different people by Barcode, can be very good to distinguish in data analysis
Different patients, and there is good dyeing effect, it can be used for dyeing extracellular and intracellular.
Described above is to illustrate and describe the present invention to specific embodiment, be used to help to understand method of the invention and
Thought.Within the spirit of the invention and the scope of protection of the claims, any modifications and changes made to the present invention, both fall within
Within protection scope of the present invention.
Claims (5)
1. a kind of detection method for mass spectrum streaming technology, comprising the following steps:
A. take cell to be detected into EP pipe, centrifuge washing cell abandons supernatant；
B. it distinguishes cell anyway: dye life or death reagent is added, 4 DEG C are incubated for 5 minutes；
C. it washs: streaming buffer is added, cell is resuspended, centrifuge washing cell abandons supernatant；
D. it fixes: fixer is added, cell is resuspended, be incubated at room temperature 10 minutes；
E. it washs: streaming buffer is added, cell is resuspended, centrifuge washing cell abandons supernatant；
F. cell is resuspended with frozen stock solution, is transferred in cryopreservation tube, is put into gradient freezing storing box, -80 DEG C of refrigerators save；
G. recovery cell: from -80 DEG C of refrigerators take out after recovery cell；
H. cell is transferred in EP pipe, streaming buffer by centrifugation washs cell, abandons supernatant；
I.Barcode: configuration Barcode mix, a kind of Barcoding mix resuspension cell is added in the sample of each EP pipe, respectively
Added Barcoding mix is different in sample；
J. wash: after streaming buffer resuspension cell is added, centrifuge washing cell abandons supernatant；
K. after different samples being mixed, centrifuge washing cell abandons supernatant；
L. close: after confining liquid is added, 4 DEG C are incubated for 20 minutes；
M. extracellular dyeing: being added the metal marker antibody of cell surface antigen, and 4 DEG C are incubated for 30 minutes；
N. it washs: streaming buffer is added, centrifuge washing cell abandons supernatant；
O. fixating reagent is configured, fixed dye DNA is put into 4 DEG C of refrigerator overnights；
P. it washs: penetrating liquid is added, centrifuge washing cell abandons supernatant；
Q. it is resuspended: Foxp3buffer is added, cell is resuspended, be incubated at room temperature 20 minutes；
R. it washs: penetrating liquid is added, centrifuge washing cell abandons supernatant；
S. dyeing intracellular: being added the metal marker antibody of antigen intracellular, and 4 DEG C are incubated for 30 minutes；
T. it washs: streaming buffer is added, centrifuge washing cell abandons supernatant；
U. machine on: sample is put into mass spectrum cell instrument after being washed with distilled water and is detected and is analyzed.
2. detection method according to claim 1, it is characterised in that: the streaming buffer formulation is containing mass concentration
1 × PBS of respectively 0.5%BSA and 0.02% Sodium azide.
3. detection method according to claim 1, it is characterised in that: in step a, c, the centrifugal rotational speed is
400g, centrifugation time are 5 minutes；In step e, h, j, k, n, p, r, t, the centrifugal rotational speed is 800g, and centrifugation time is 5 points
Clock.
4. detection method according to claim 1, it is characterised in that: Barcode mix is selected from 104Pd/ in the step i
105Pd,104Pd/106Pd,104Pd/108Pd,104Pd/110Pd,105Pd/106Pd,105Pd/108Pd,105Pd/
110Pd,106Pd/108Pd,106Pd/110Pd,108Pd/110Pd。
5. detection method according to claim 1, it is characterised in that: the step l, confining liquid contain Human IgG,
Mouse IgG, Hamster IgG and Rat IgG.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110412287A (en) * | 2019-07-11 | 2019-11-05 | 上海宸安生物科技有限公司 | One kind being based on single celled immunocyte parting quantitative analysis method |
| CN110567861A (en) * | 2019-09-09 | 2019-12-13 | 浙江普罗亭健康科技有限公司 | kit for screening antigenic peptide with immunogenicity based on mass flow detection technology and detection method |
| CN110608991A (en) * | 2019-09-09 | 2019-12-24 | 浙江普罗亭健康科技有限公司 | Cell cycle detection kit based on mass flow detection technology and detection method |
| CN111189761A (en) * | 2020-01-10 | 2020-05-22 | 浙江普罗亭健康科技有限公司 | Cell signal channel detection kit based on mass spectrometry flow technology and detection method |
| CN113125733A (en) * | 2021-04-16 | 2021-07-16 | 浙江普罗亭健康科技有限公司 | 42 antibody kit for monitoring human immune state and application thereof |
| CN113588523A (en) * | 2021-07-26 | 2021-11-02 | 浙江大学 | Frame structure-based nano-particles for mass flow cytometry and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107314965A (en) * | 2017-04-26 | 2017-11-03 | 马鞍山易廷生物科技有限公司 | The Sample pretreatment method detected based on streaming combination ICP MS single cell proteins |
| CN107796748A (en) * | 2017-09-28 | 2018-03-13 | 上海交通大学 | A kind of detection method for unicellular mass spectrum flow cytometry |
| CN207379796U (en) * | 2017-10-24 | 2018-05-18 | 江苏立峰生物科技有限公司 | Full-automatic microcomputer controls film-making dyeing integration machine |
| CN108398560A (en) * | 2017-12-28 | 2018-08-14 | 兰州大学 | A kind of CD133 mass spectrums streaming antibody, preparation method and application |
-
2018
- 2018-10-10 CN CN201811180002.8A patent/CN109323908A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107314965A (en) * | 2017-04-26 | 2017-11-03 | 马鞍山易廷生物科技有限公司 | The Sample pretreatment method detected based on streaming combination ICP MS single cell proteins |
| CN107796748A (en) * | 2017-09-28 | 2018-03-13 | 上海交通大学 | A kind of detection method for unicellular mass spectrum flow cytometry |
| CN207379796U (en) * | 2017-10-24 | 2018-05-18 | 江苏立峰生物科技有限公司 | Full-automatic microcomputer controls film-making dyeing integration machine |
| CN108398560A (en) * | 2017-12-28 | 2018-08-14 | 兰州大学 | A kind of CD133 mass spectrums streaming antibody, preparation method and application |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110412287A (en) * | 2019-07-11 | 2019-11-05 | 上海宸安生物科技有限公司 | One kind being based on single celled immunocyte parting quantitative analysis method |
| CN110567861A (en) * | 2019-09-09 | 2019-12-13 | 浙江普罗亭健康科技有限公司 | kit for screening antigenic peptide with immunogenicity based on mass flow detection technology and detection method |
| CN110608991A (en) * | 2019-09-09 | 2019-12-24 | 浙江普罗亭健康科技有限公司 | Cell cycle detection kit based on mass flow detection technology and detection method |
| CN110567861B (en) * | 2019-09-09 | 2021-12-21 | 浙江普罗亭健康科技有限公司 | Kit for screening antigenic peptide with immunogenicity based on mass flow detection technology and detection method |
| CN110608991B (en) * | 2019-09-09 | 2022-04-29 | 浙江普罗亭健康科技有限公司 | Cell cycle detection kit based on mass flow detection technology and detection method |
| CN111189761A (en) * | 2020-01-10 | 2020-05-22 | 浙江普罗亭健康科技有限公司 | Cell signal channel detection kit based on mass spectrometry flow technology and detection method |
| CN113125733A (en) * | 2021-04-16 | 2021-07-16 | 浙江普罗亭健康科技有限公司 | 42 antibody kit for monitoring human immune state and application thereof |
| CN113588523A (en) * | 2021-07-26 | 2021-11-02 | 浙江大学 | Frame structure-based nano-particles for mass flow cytometry and preparation method thereof |
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