CN109316268A - A kind of bioresorbable slide fastener scaffold and preparation method - Google Patents
A kind of bioresorbable slide fastener scaffold and preparation method Download PDFInfo
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- CN109316268A CN109316268A CN201811298374.0A CN201811298374A CN109316268A CN 109316268 A CN109316268 A CN 109316268A CN 201811298374 A CN201811298374 A CN 201811298374A CN 109316268 A CN109316268 A CN 109316268A
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- slide fastener
- bioresorbable
- structure body
- reticular structure
- mold
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
- A61F2/82—Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
- A61F2/86—Stents in a form characterised by the wire-like elements; Stents in the form characterised by a net-like or mesh-like structure
- A61F2/90—Stents in a form characterised by the wire-like elements; Stents in the form characterised by a net-like or mesh-like structure characterised by a net-like or mesh-like structure
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/04—Macromolecular materials
- A61L31/06—Macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/14—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/14—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L31/146—Porous materials, e.g. foams or sponges
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/14—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L31/148—Materials at least partially resorbable by the body
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2210/00—Particular material properties of prostheses classified in groups A61F2/00 - A61F2/26 or A61F2/82 or A61F9/00 or A61F11/00 or subgroups thereof
- A61F2210/0004—Particular material properties of prostheses classified in groups A61F2/00 - A61F2/26 or A61F2/82 or A61F9/00 or A61F11/00 or subgroups thereof bioabsorbable
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2240/00—Manufacturing or designing of prostheses classified in groups A61F2/00 - A61F2/26 or A61F2/82 or A61F9/00 or A61F11/00 or subgroups thereof
- A61F2240/001—Designing or manufacturing processes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
Abstract
The invention belongs to field of medical device, disclose a kind of bioresorbable slide fastener scaffold and preparation method, bioresorbable slide fastener scaffold is provided with reticular structure body, outside support, upside slide fastener, downside slide fastener, bracket buckle, internal stent, internal stent is laminated structure, is embedded in by reticular structure body；Reticular structure body is quadrangle and triangle shuffling, and upper and lower two sides are quadrilateral structure, and centre is triangular structure；Upside slide fastener and downside slide fastener are embedded in internal stent two sides, for raised slide fastener；Outside support is connected with internal stent, and there is bracket buckle on surface.Bracket buckle of the present invention is the button length of protrusion between 0.6-1mm, internal stent and outside support material generate new polymer etc. by the high molecular polymers such as polylactic acid, polycaprolactone and different polymer copolymerizations, blending etc. and constitute, there is good biocompatibility, bracket is transported to by lesion locations by transmission device.
Description
Technical field
The invention belongs to field of medical device more particularly to a kind of bioresorbable slide fastener scaffold and preparation methods.
Background technique
Currently, the prior art commonly used in the trade is such that
Bracket is generally implanted to the lesions narrow positions such as tracheae, society, blood vessel, can effectively maintain the unobstructed of official jargon.It passes
Bracket on system can be divided into self-expandable stent and balloon expandable stent, the former is mainly the high people containing memorial alloy from chemical combination
Object is pre-filled between conduit, maintains the unimpeded of official jargon by the expansion mechanism of itself after being imported into lesion；The latter's sheet
Body from swollen characteristic, is not preset on sacculus first, reaches lesion according to the guiding of director, is generated by the expansion of sacculus
Plasticity maintains the unobstructed of official jargon to be affixed on official jargon inner wall.
Biological absorbable support is fundamental characteristics and the requirement for meeting bracket, can gradually degrade and be inhaled in coronary artery
The bracket of receipts.Conventional metals bracket exist damage blood vessel endothelium, in-stent restenosis, thrombosis, influence auxiliary examination and after
The problems such as continuous treatment, influence the long term survival quality and survival rate of stenter to implant postoperative patient person, the appearance of biological absorbable support
New thinking is brought to solve these problems with development, also has new demand to material.Currently used for biological absorbable support
Material includes biologically absorbable polymer and metal alloy, the former mainly includes poly-lactic acid ester, polytyrosinecarbonates, polyanhydride ester
And polyorthoester, the latter mainly include magnesium alloy and ferroalloy.
Timbering material should have degradation speed appropriate, and (6~12 months) drop in a predictive manner in limited period
Solution, without any residue.Timbering material and catabolite should have biocompatibility, nontoxic, and it is anti-will not to generate apparent inflammation
It answers.There is high-tensile and strain-failure force before timbering material degradation, so that can be made into has easily transmission and flexibility special
The lower shrinkage of point, balloon expandable stent；With enough radial support power and mechanical property to be supported during partial polymerization
Blood vessel.
Problem of the existing technology is in summary:
The prior art easily forms thrombus and needs long-term anti-platelet therapy；It is lifelong to be detained in vivo, life after being on sale throughout；It radiates
It is impacted when treatment.
In the prior art, since bioresorbable slide fastener scaffold is porous structure, it is not suitable for clinically repairing application；
Timbering material is single in the prior art, and biocompatibility and mechanical property wait to improve.
Summary of the invention
In view of the problems of the existing technology, the present invention provides a kind of bioresorbable slide fastener scaffold and preparation methods.
The invention is realized in this way a kind of preparation method of bioresorbable slide fastener scaffold, the slippery buckle biological can be inhaled
The preparation method of revenue and expenditure frame includes:
Polylactic acid or polycaprolactone high molecular polymer are woven into quadrangularly by yarn prepared by electrostatic spinning technique
With triangle shuffling reticular structure body, two sides are quadrilateral structure to reticular structure body up and down, and centre is triangular structure, for increasing
Strong plasticity and support degree；
Polylactic acid or polycaprolactone high molecular polymer are dissolved in organic solvent, stirring and dissolving, obtain outside support and
Internal stent solution；
Reticular structure body, outside support and internal stent solution are placed in mold, are prepared into using thermally induced phase separation
Bioresorbable slide fastener scaffold；
By mold by the upside slide fastener of the outside support two sides of bioresorbable slide fastener scaffold Tooth preparation casting protrusion and
Downside slide fastener；
Outside support surface casting bracket buckle.
Further, the yarn or be one in poly (glycolide-lactide) yarn PLGA, polyethylene terephtalate
Kind.
Further, the quality concentration expressed in percentage by volume of the outside support and internal stent solution is 8%-15%；
The organic solvent is tetrahydrofuran；The stirring and dissolving time is 3-4h, rate 300-450r/min, solution temperature
It is 65 DEG C.
Further, the mold is polytetrafluorethyletubular tubular mold；Mold include plug, mandrel, outside support hollow tube,
Internal stent hollow tube, upside slide fastener capillary hollow, downside slide fastener capillary hollow, bracket buckle capillary hollow；Mandrel is put
It is placed in internal stent hollow tube；Internal stent hollow tube is embedded in reticular structure body；It is carried out with the both ends that plug fills in mold shell
Sealing；Upside slide fastener capillary hollow, downside slide fastener capillary hollow are connected to mandrel；The external branch of bracket buckle capillary hollow connection
Frame hollow tube.
Further, the thermally induced phase separation specifically: -80 DEG C of freezing 18h take out bioresorbable slide fastener scaffold and are put into
It replaces solvent 1-4 days, is freeze-dried 1-4 days in water.
Further, the preparation method of reticular structure body includes: to pour into chitosan solution in collagen solution, with homogenizer ice
It is mixed under water bath condition, gel will be mixed and vacuumized, deflation makes bubble breaking；It is centrifuged later；The injection of bubble-free gel will be mixed
Two sides are quadrilateral structure up and down for quadrangle and triangle shuffling mold, quadrangle and triangle shuffling mold, and centre is triangle
Shape structure；And the copper plug to match with the mold internal diameter is filled in quadrangle and triangle shuffling mold one end；Quadrangle and
Triangle shuffling mold is packed into vertical drop frame；After being totally immersed into liquid nitrogen, quadrangle and triangle shuffling mold, removal are taken out
Copper plug；It is freeze-dried with freeze drier；The reticular structure body made is removed from the molds, the friendship of Geniposide ethanol solution is put into
Connection；After crosslinking, with distilled water repeated washing；The reticular structure body of cleaning is taken out, freeze-drying；Reticular structure body battle array is made.
Further, it is 3mg/ml that 0mg~250mg, which takes chitosan, 0mg~350mg type i collagen to be dissolved in the concentration of 5ml respectively,
In the acetum of~6mg/ml, 4 DEG C, dissolve 24 hours；Chitosan solution is poured into collagen solution, with homogenizer ice-water bath
Under the conditions of mix, mixing speed 2500rpm~3000rpm；Gel will be mixed and first vacuumize 5min with vacuum pump, deflation makes bubble
It is broken, it is repeated 4 times~5 times；Centrifuge 5000rpm is centrifuged 3min~8min later；Bubble-free gel injection internal diameter, which will be mixed, is
The quadrangle and triangle shuffling mold of 1.5~2.0mm.
Further, quadrangle and triangle shuffling mold are packed into vertical drop frame, under 1mm/min~6mm/min speed
Drop；It is freeze-dried 18h~36h with freeze drier, until dry；By the reticular structure body made from quadrangle and triangle shuffling
It is taken out in mold, is put into 4 DEG C of 1% Geniposide ethanol solution of 20ml and is crosslinked 4 hours~36 hours；After crosslinking, distilled water is used
Repetition washes 3 times~8 times, 1 hour/time；The reticular structure body of cleaning is taken out, -80 DEG C is put into and freezes 30 minutes~90 minutes, put
Enter to be pre-chilled in freeze dryer and be lyophilized 18 hours~36 hours；Reticular structure body is made.
Further, the button and length that the bracket buckle is protrusion are between 0.6-1mm；
Reticular structure body is equally distributed, mesh diameter 0.2-0.5mm, in circle, ellipse, quadrangle, triangle
One kind.
Another object of the present invention is to provide a kind of bioresorbable slide fastener scaffolds to be provided with reticular structure body；
The internal stent of reticular structure body insertion laminated structure；
The reticular structure body is quadrangle and triangle shuffling, and upper and lower two sides are quadrilateral structure, and centre is triangle
Structure, for enhancing plasticity and support degree；
Upside slide fastener and downside slide fastener are embedded in internal stent two sides；Outside support is connected with internal stent, and there is bracket on surface
Button；Bracket buckle and slide fastener are used to fix reticular structure body together.
In conclusion advantages of the present invention and good effect are as follows:
The present invention keeps its stronger plastic by the way that quadrangle and triangle shuffling can be increased to the support degree of bracket
Property, the division of labor can preferably be cooperated with slide fastener by increasing multiple bracket buckles, can make bracket according to the different position of official jargon to reach
Corresponding effect, bracket buckle are the button length of protrusion between 0.6-1mm, internal stent and outside support material by polylactic acid,
The high molecular polymers such as polycaprolactone and different polymer copolymerizations, blending etc. generate the composition such as new polymer, have very well
Biocompatibility.
It is 10mm-100mm that the present invention, which can prepare length by electrostatic spinning, and internal diameter is that the slippery buckle biological of 1mm-8mm can
Absorb bracket.It can promote biological absorbable and improve procedure efficiency.
The present invention combines knitting skill with Thermal inactive technology, and biocompatibility is prepared and mechanical property is excellent
Different bracket；And this has porous structure, can promote adherency, the growth of cell, promote the Regeneration and Repair of damaged tissues.
Detailed description of the invention
Fig. 1 is bioresorbable slide fastener scaffold structural schematic diagram provided in an embodiment of the present invention；
In figure: 1, reticular structure body；2, outside support；3, upside slide fastener；4, downside slide fastener；5, bracket buckle；6, internal branch
Frame
Fig. 2 is the preparation method of bioresorbable slide fastener scaffold provided in an embodiment of the present invention
Flow chart.
Specific embodiment
In order to further understand the content, features and effects of the present invention, the following examples are hereby given, and cooperate attached drawing
Detailed description are as follows.
As shown in Figure 1, bioresorbable slide fastener scaffold provided in an embodiment of the present invention, comprising: reticular structure body 1, outside
Bracket 2, upside slide fastener 3, downside slide fastener 4, bracket buckle 5, internal stent 6；
The internal stent 6 is laminated structure, is embedded in by reticular structure body 1；The reticular structure body 1 is quadrangle
With triangle shuffling, upper and lower two sides are quadrilateral structure, and centre is triangular structure, for enhancing plasticity and support degree；
The upside slide fastener 3 and downside slide fastener 4 are embedded in 6 two sides of internal stent, for raised slide fastener；The outside support 2 and internal stent 6
It is connected, there is bracket buckle 5 on surface；The bracket buckle 5 and slide fastener are used to fix bracket together.
Below with reference to concrete analysis, the invention will be further described.
The preparation method of bioresorbable slide fastener scaffold provided in an embodiment of the present invention, comprising:
S101: polylactic acid or polycaprolactone high molecular polymer are woven into four by yarn prepared by electrostatic spinning technique
Side shape and triangle shuffling reticular structure body, two sides are quadrilateral structure to reticular structure body up and down, and centre is triangular structure, is used
In enhancing plasticity and support degree；
S102: polylactic acid or polycaprolactone high molecular polymer being dissolved in organic solvent, stirring and dissolving, obtain external branch
Frame and internal stent solution；
S103: reticular structure body, outside support and internal stent solution are placed in mold, using Thermal inactive legal system
It is standby to obtain bioresorbable slide fastener scaffold；It is by mold that the outside support two sides casting of bioresorbable slide fastener scaffold Tooth preparation is convex
The upside slide fastener and downside slide fastener risen；Outside support surface casting bracket buckle.
The yarn is one of poly (glycolide-lactide) yarn PLGA, polyethylene terephtalate.
The quality concentration expressed in percentage by volume of the outside support and internal stent solution is 8%-15%；
The organic solvent is tetrahydrofuran；The stirring and dissolving time is 3-4h, rate 300-450r/min, solution temperature
It is 65 DEG C.
The mold is polytetrafluorethyletubular tubular mold；Mold includes plug, mandrel, outside support hollow tube, internal branch
Frame hollow tube, upside slide fastener capillary hollow, downside slide fastener capillary hollow, bracket buckle capillary hollow；In mandrel is placed into
Portion's bracket hollow tube；Internal stent hollow tube is embedded in reticular structure body；It is sealed with the both ends that plug fills in mold shell；On
Break away button capillary hollow, downside slide fastener capillary hollow connection mandrel；It is hollow that bracket buckle capillary hollow connects outside support
Pipe.
The thermally induced phase separation specifically: -80 DEG C of freezing 18h take out bioresorbable slide fastener scaffold and set into the water
It changes solvent 1-4 days, is freeze-dried 1-4 days.
The preparation method of reticular structure body includes: to pour into chitosan solution in collagen solution, with homogenizer ice-water bath item
It is mixed under part, gel will be mixed and vacuumized, deflation makes bubble breaking；It is centrifuged later；Bubble-free gel will be mixed and inject quadrangle
With triangle shuffling mold, two sides are quadrilateral structure up and down for quadrangle and triangle shuffling mold, and centre is triangular structure；
And the copper plug to match with the mold internal diameter is filled in quadrangle and triangle shuffling mold one end；Quadrangle and triangle are mixed
It compiles mold and is packed into vertical drop frame；After being totally immersed into liquid nitrogen, quadrangle and triangle shuffling mold are taken out, copper removal plug is removed；With
Freeze drier freeze-drying；The reticular structure body made is removed from the molds, the crosslinking of Geniposide ethanol solution is put into；Crosslinking
After, with distilled water repeated washing；The reticular structure body of cleaning is taken out, freeze-drying；Reticular structure body battle array is made.
It is 3mg/ml~6mg/ that 0mg~250mg, which takes chitosan, 0mg~350mg type i collagen to be dissolved in the concentration of 5ml respectively,
In the acetum of ml, 4 DEG C, dissolve 24 hours；Chitosan solution is poured into collagen solution, under the conditions of homogenizer ice-water bath
It mixes, mixing speed 2500rpm~3000rpm；Gel will be mixed and first vacuumize 5min with vacuum pump, deflation makes bubble breaking,
It is repeated 4 times~5 times；Centrifuge 5000rpm is centrifuged 3min~8min later；Will mix bubble-free gel injection internal diameter be 1.5~
The quadrangle and triangle shuffling mold of 2.0mm.
Quadrangle and triangle shuffling mold are packed into vertical drop frame, with the decline of 1mm/min~6mm/min speed；With cold
Lyophilizer is freeze-dried 18h~36h, until dry；By the reticular structure body made from quadrangle and triangle shuffling mold
It takes out, is put into 4 DEG C of 1% Geniposide ethanol solution of 20ml and is crosslinked 4 hours~36 hours；After crosslinking, repeat to wash with distilled water
3 times~8 times, 1 hour/time；The reticular structure body of cleaning is taken out, -80 DEG C is put into and freezes 30 minutes~90 minutes, be put into pre-cooling
It is lyophilized 18 hours~36 hours in freeze dryer；Reticular structure body is made.
The bracket buckle is raised button and length between 0.6-1mm；
Reticular structure body is equally distributed, mesh diameter 0.2-0.5mm, in circle, ellipse, quadrangle, triangle
One kind.
The above is only the preferred embodiments of the present invention, and is not intended to limit the present invention in any form,
Any simple modification made to the above embodiment according to the technical essence of the invention, equivalent variations and modification, belong to
In the range of technical solution of the present invention.
Claims (10)
1. a kind of preparation method of bioresorbable slide fastener scaffold, which is characterized in that the system of the bioresorbable slide fastener scaffold
Preparation Method includes:
Polylactic acid or polycaprolactone high molecular polymer are woven into quadrangularly and three by yarn prepared by electrostatic spinning technique
Angular shuffling reticular structure body, two sides are quadrilateral structure to reticular structure body up and down, and centre is triangular structure, can for enhancing
Plasticity and support degree；
Polylactic acid or polycaprolactone high molecular polymer are dissolved in organic solvent, stirring and dissolving obtains outside support and inside
Bracket solution；
Reticular structure body, outside support and internal stent solution are placed in mold, slide fastener is prepared into using thermally induced phase separation
Biological absorbable support；
Pass through the upside slide fastener and downside that mold is raised by the outside support two sides casting of bioresorbable slide fastener scaffold Tooth preparation
Slide fastener；
Outside support surface casting bracket buckle.
2. the preparation method of bioresorbable slide fastener scaffold as described in claim 1, which is characterized in that the yarn is poly-
One of second lactide yarn PLGA, polyethylene terephtalate.
3. the preparation method of bioresorbable slide fastener scaffold as described in claim 1, which is characterized in that
The quality concentration expressed in percentage by volume of the outside support and internal stent solution is 8%-15%；
The organic solvent is tetrahydrofuran；The stirring and dissolving time is 3-4h, rate 300-450r/min, solution temperature 65
℃。
4. the preparation method of bioresorbable slide fastener scaffold as described in claim 1, which is characterized in that the mold is poly- four
Vinyl fluoride tubular die；Mold includes plug, mandrel, outside support hollow tube, internal stent hollow tube, the hollow hair of upside slide fastener
Tubule, downside slide fastener capillary hollow, bracket buckle capillary hollow；Mandrel is placed into internal stent hollow tube；In internal stent
Blank pipe is embedded in reticular structure body；It is sealed with the both ends that plug fills in mold shell；Upside slide fastener capillary hollow, lower sideslip
It detains capillary hollow and is connected to mandrel；Bracket buckle capillary hollow connects outside support hollow tube.
5. the preparation method of bioresorbable slide fastener scaffold as described in claim 1, which is characterized in that
The thermally induced phase separation specifically: -80 DEG C of freezing 18h, taking-up bioresorbable slide fastener scaffold are replaced molten into the water
It agent 1-4 days, is freeze-dried 1-4 days.
6. the preparation method of bioresorbable slide fastener scaffold as described in claim 1, which is characterized in that
The preparation method of reticular structure body includes: to pour into chitosan solution in collagen solution, under the conditions of homogenizer ice-water bath
It mixes, gel will be mixed and vacuumized, deflation makes bubble breaking；It is centrifuged later；Bubble-free gel will be mixed and inject quadrangle and three
Two sides are quadrilateral structure up and down for angular shuffling mold, quadrangle and triangle shuffling mold, and centre is triangular structure；And
The copper plug to match with the mold internal diameter is filled in quadrangle and triangle shuffling mold one end；Quadrangle and triangle shuffling mould
Tool is packed into vertical drop frame；After being totally immersed into liquid nitrogen, quadrangle and triangle shuffling mold are taken out, copper removal plug is removed；With freezing
Drying machine freeze-drying；The reticular structure body made is removed from the molds, the crosslinking of Geniposide ethanol solution is put into；Crosslinking finishes
Afterwards, with distilled water repeated washing；The reticular structure body of cleaning is taken out, freeze-drying；Reticular structure body battle array is made.
7. the preparation method of bioresorbable slide fastener scaffold as claimed in claim 6, which is characterized in that
0mg~250mg takes chitosan, 0mg~350mgI Collagen Type VI to be dissolved in the vinegar that the concentration of 5ml is 3mg/ml~6mg/ml respectively
In acid solution, 4 DEG C, dissolve 24 hours；Chitosan solution is poured into collagen solution, with being mixed under the conditions of homogenizer ice-water bath,
Mixing speed 2500rpm~3000rpm；Gel will be mixed and first vacuumize 5min with vacuum pump, deflation makes bubble breaking, repeats 4
It is secondary~5 times；Centrifuge 5000rpm is centrifuged 3min~8min later；It is 1.5~2.0mm that bubble-free gel injection internal diameter, which will be mixed,
Quadrangle and triangle shuffling mold.
8. the preparation method of bioresorbable slide fastener scaffold as claimed in claim 6, which is characterized in that quadrangle and triangle
Shuffling mold is packed into vertical drop frame, with the decline of 1mm/min~6mm/min speed；With freeze drier freeze-drying 18h~
36h, until dry；The reticular structure body made is taken out from quadrangle and triangle shuffling mold, is put into 20ml1% Geniposide
4 DEG C of ethanol solution are crosslinked 4 hours~36 hours；After crosslinking, repeated to wash 3 times~8 times with distilled water, 1 hour/time；It will be clear
The reticular structure body washed takes out, and is put into -80 DEG C and freezes 30 minutes~90 minutes, is put into pre-cooling freeze dryer and is lyophilized 18 hours~36
Hour；Reticular structure body is made.
9. the preparation method of bioresorbable slide fastener scaffold as described in claim 1, which is characterized in that the bracket buckle is convex
The button and length risen is between 0.6-1mm；
Reticular structure body is equally distributed, mesh diameter 0.2-0.5mm, for one in circle, ellipse, quadrangle, triangle
Kind.
10. a kind of bioresorbable slide fastener scaffold of the preparation method using bioresorbable slide fastener scaffold described in claim 1,
It is characterized in that, the bioresorbable slide fastener scaffold is provided with reticular structure body；
The internal stent of reticular structure body insertion laminated structure；
The reticular structure body is quadrangle and triangle shuffling, and upper and lower two sides are quadrilateral structure, and centre is triangular structure,
For enhancing plasticity and support degree；
Upside slide fastener and downside slide fastener are embedded in internal stent two sides；Outside support is connected with internal stent, and there is bracket buckle on surface；Branch
Frame is buckled and slide fastener is used to fix reticular structure body together.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
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| CN109893305A (en) * | 2019-04-16 | 2019-06-18 | 南京医科大学附属逸夫医院 | A kind of tissue engineering bracket with subregion |
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