CN109307673A - A kind of zinc-oxide nano enzyme and its application in preparation quickly detection milk in melamine test paper - Google Patents
A kind of zinc-oxide nano enzyme and its application in preparation quickly detection milk in melamine test paper Download PDFInfo
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- CN109307673A CN109307673A CN201811389073.9A CN201811389073A CN109307673A CN 109307673 A CN109307673 A CN 109307673A CN 201811389073 A CN201811389073 A CN 201811389073A CN 109307673 A CN109307673 A CN 109307673A
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- zinc
- quantum dot
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- oxide nano
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N2021/7756—Sensor type
- G01N2021/7759—Dipstick; Test strip
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- Chemical Kinetics & Catalysis (AREA)
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- Life Sciences & Earth Sciences (AREA)
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The present invention relates to a kind of zinc-oxide nano enzyme and its applications in preparation quickly detection milk in melamine test paper, the test paper includes blotting paper and reaction film, the reaction film is coated with the detection line of the above-mentioned zinc-oxide nano enzyme based on modified by graphene quantum dot, the material of the reaction film is cellulose membrane, preferably cellulose acetate film, nitrocellulose filter, glass fibre element film or polyester cellulose film.
Description
Technical field
The invention belongs to nano material and melamine detection fields, and in particular to a kind of zinc-oxide nano enzyme and its make
Application in standby quickly detection milk in melamine test paper.
Background technique
Melamine (Melamine) is a kind of triazines nitrogen heterocyclic ring organic compound, is slightly soluble in water, be soluble in methanol,
The content of glycerol, nitrogen accounts for about 66%, also higher than protein, and cheap, so, some illegal retailers are in order to seek benefit
Benefit is added into dairy products or food, causes the illusion of high protein.However, melamine is forbidden adding for food,
Long-term intake melamine not only results in reproduction, the damage of urinary system, failure, or even also will cause death.Therefore, accurately
The melamine being quickly detected from milk is for guaranteeing that food safety has very important significance.The present invention provides one kind
Zinc-oxide nano enzyme based on modified by graphene quantum dot can be used for preparing the test paper of melamine in quickly detection milk.
Summary of the invention
The present invention provides a kind of zinc-oxide nano enzyme based on modified by graphene quantum dot, it is characterised in that described to be based on stone
The preparation method of the zinc-oxide nano enzyme of black alkene quantum dot modification includes the following steps:
Graphene quantum dot solution is added into zinc nitrate solution, after stirring 10min, it is molten that alkali metal hydroxide is added
Liquid continues after stirring 10min, is warming up to 140 DEG C, reacts 12 hours, cooled to room temperature, filtering, precipitating deionized water,
After ethanol washing, 10h is dried in vacuo at 80 DEG C to get the zinc-oxide nano enzyme based on modified by graphene quantum dot.
The concentration of the zinc nitrate solution is 0.1mol/L, and the concentration of graphene quantum dot solution is 1.0mg/mL, alkali gold
The concentration for belonging to hydroxide solution is 0.1mol/L, and every mM of zinc nitrate uses graphene quantum dot solution 5mL, uses alkali gold
Belong to hydroxide solution 10mL.
The preferred sodium hydroxide of the alkali metal hydroxide or potassium hydroxide.It is described reaction preferably in a high pressure reaction kettle into
Row.
Another embodiment of the present invention provides the above-mentioned zinc-oxide nano enzyme based on modified by graphene quantum dot and is detecting
Application in melamine.Especially in the application of detection melamine in dairy products.
Another embodiment of the present invention provides the above-mentioned zinc-oxide nano enzyme based on modified by graphene quantum dot and is preparing
Quickly detect the application of melamine test paper in milk.
Another embodiment of the present invention provides one kind and quickly detects melamine test paper in milk, it is characterised in that described
Test paper includes blotting paper and reaction film, and the reaction film is coated with the above-mentioned zinc-oxide nano enzyme based on modified by graphene quantum dot
Detection line, the material of the reaction film is cellulose membrane, preferably cellulose acetate film, nitrocellulose filter, glass fibre element
Film or polyester cellulose film.
The blotting paper is blotting paper commonly used in the art, thickness 0.3-0.5mm, rate of water absorption 5-10s/4cm, water absorption
300-500mg/cm2。
Compared with the prior art, the advantages of the present invention are as follows: the present invention is based on modified by graphene quantum dot to obtain zinc oxide
Nano enzyme, the test paper of preparation can be used for directly quickly detecting the middle melamine of the dairy products such as milk, and detection limit reaches 25ppm.
Detailed description of the invention
Fig. 1 is the SEM figure of product A.
Specific embodiment
For the ease of a further understanding of the present invention, examples provided below has done more detailed description to it.But
It is that these embodiments are only not supposed to be a limitation to the present invention or implementation principle for better understanding invention, reality of the invention
The mode of applying is not limited to the following contents.
Embodiment 1
Graphene quantum dot solution (1.0mg/mL, 5mL) is added in zinc nitrate solution (0.1mol/L, 10mL), stirring
It after 10min, is added sodium hydroxide solution (0.1mol/L, 10mL), continues after stirring 10min, be warming up to 140 DEG C, reaction 12 is small
When, cooled to room temperature, filtering is precipitated with after deionized water, ethanol washing, and vacuum drying 10h is at 80 DEG C to get described
Zinc-oxide nano enzyme (78mg, hereinafter referred to as product A) based on modified by graphene quantum dot.
Embodiment 2
Graphene quantum dot solution (1.0mg/mL, 5mL) is added in zinc nitrate solution (0.1mol/L, 10mL), stirring
It after 10min, is added potassium hydroxide solution (0.1mol/L, 10mL), continues after stirring 10min, be warming up to 140 DEG C, reaction 12 is small
When, cooled to room temperature, filtering is precipitated with after deionized water, ethanol washing, and vacuum drying 10h is at 80 DEG C to get described
Zinc-oxide nano enzyme based on modified by graphene quantum dot (pattern is consistent with product A under scanning electron microscope).
Embodiment 3
Firstly, product A is sprayed on reaction film (nitrocellulose filter or polyester cellulose film) using film metal spraying mark machine is drawn
On detection line is made；Then reaction film and blotting paper are pasted, the test strips for needing specification are cut into dedicated cutting machine,
(such as 4.0 × 55mm).The blotting paper is blotting paper commonly used in the art, thickness 0.3-0.5mm, rate of water absorption 5-10s/
4cm, water absorption 300-500mg/cm2。
Embodiment 4
Fresh milk is taken, appropriate melamine is added, is configured to the sample to be tested that content of melamine is 200mg/L, then
Dilution is made into the sample to be tested that concentration is 100,50,25,12.5,6.25mg/L respectively.
The sample to be tested of various concentration and fresh milk (blank control) are added drop-wise to the test strips of the preparation of embodiment 3 respectively
On, observed after 30s, be added dropwise concentration be 200,100,50, occur clearly at the test strips detection line of 25mg/L sample to be tested
Blue detection line, only dropwise addition fresh milk (blank control) and concentration are the test strips detection of 12.5,6.25mg/L sample to be tested
Do not occur blue detection line at line.Be repeated 5 times test, be added dropwise concentration be 200,100,50,25,6.25mg/L sample to be tested and fresh
The testing result of the test strips of milk (blank control) is consistent；Wherein having 3 tests that concentration is added dropwise is 12.5mg/L sample to be tested
Indistinctly there is detection line at the detection line of test strips.The result shows that test strips of the present invention detect the detection of melamine in milk
It is limited to 25mg/L (being equivalent to 25ppm).
Claims (10)
1. a kind of zinc-oxide nano enzyme based on modified by graphene quantum dot, it is characterised in that described to be repaired based on graphene quantum dot
The preparation method of the zinc-oxide nano enzyme of decorations includes the following steps:
Graphene quantum dot solution is added into zinc nitrate solution, after stirring 10min, alkali hydroxide soln is added, after
After continuous stirring 10min, 140 DEG C are warming up to, is reacted 12 hours, cooled to room temperature, filtering, precipitating deionized water, ethyl alcohol
After washing, 10h is dried in vacuo at 80 DEG C to get the zinc-oxide nano enzyme based on modified by graphene quantum dot.
2. the zinc-oxide nano enzyme described in claim 1 based on modified by graphene quantum dot, it is characterised in that the zinc nitrate
The concentration of solution is 0.1mol/L, and the concentration of graphene quantum dot solution is 1.0mg/mL, alkali hydroxide soln it is dense
Degree is 0.1mol/L, and every mM of zinc nitrate uses graphene quantum dot solution 5mL, uses alkali hydroxide soln
10mL。
3. the described in any item zinc-oxide nano enzymes based on modified by graphene quantum dot of claim 1-2, it is characterised in that institute
State the preferred sodium hydroxide of alkali metal hydroxide or potassium hydroxide.
4. the described in any item zinc-oxide nano enzymes based on modified by graphene quantum dot of claim 1-3 are in detection melamine
In application.
5. application as claimed in claim 4, it is characterised in that the application in detection melamine in dairy products.
6. the described in any item zinc-oxide nano enzymes based on modified by graphene quantum dot of claim 1-3 are in preparation quickly detection
Application in milk in melamine test paper.
7. the test paper of melamine in a kind of quickly detection milk, it is characterised in that the test paper includes blotting paper and reaction film,
The reaction film is coated with the detection line of the above-mentioned zinc-oxide nano enzyme based on modified by graphene quantum dot.
8. test paper as claimed in claim 7, it is characterised in that the material of the reaction film is cellulose membrane, preferably cellulose acetate
Film, nitrocellulose filter, glass fibre element film or polyester cellulose film.
9. the described in any item test paper of claim 7-8, it is characterised in that the blotting paper is blotting paper commonly used in the art, thick
Spend 0.3-0.5mm, rate of water absorption 5-10s/4cm, water absorption 300-500mg/cm2。
10. the preparation method of the described in any item zinc-oxide nano enzymes based on modified by graphene quantum dot of claim 1-3.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811389073.9A CN109307673A (en) | 2018-11-20 | 2018-11-20 | A kind of zinc-oxide nano enzyme and its application in preparation quickly detection milk in melamine test paper |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811389073.9A CN109307673A (en) | 2018-11-20 | 2018-11-20 | A kind of zinc-oxide nano enzyme and its application in preparation quickly detection milk in melamine test paper |
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| Publication Number | Publication Date |
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| CN109307673A true CN109307673A (en) | 2019-02-05 |
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| CN201811389073.9A Pending CN109307673A (en) | 2018-11-20 | 2018-11-20 | A kind of zinc-oxide nano enzyme and its application in preparation quickly detection milk in melamine test paper |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102156118A (en) * | 2011-03-22 | 2011-08-17 | 中国科学院长春应用化学研究所 | Method for detecting melamine |
| US20120140216A1 (en) * | 2010-12-07 | 2012-06-07 | Sony Corporation | Method for evaluation of oxide semiconductor electrode, apparatus for evaluation of oxide semiconductor electrode, and apparatus for production of oxide semiconductor electrode |
| CN104098088A (en) * | 2014-06-17 | 2014-10-15 | 华南理工大学 | Preparation method for nano-zinc oxide modified graphene hybrid material |
| CN104941643A (en) * | 2015-06-16 | 2015-09-30 | 北京科技大学 | Preparation method of Ag-GQDs(Graphene Quantum Dots)/ZnO ternary photocatalyst |
| CN107321375A (en) * | 2017-07-13 | 2017-11-07 | 扬州工业职业技术学院 | A kind of SiO2/ZnO/g‑C3N4Nano material and its application in reduction of hexavalent chromium |
-
2018
- 2018-11-20 CN CN201811389073.9A patent/CN109307673A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120140216A1 (en) * | 2010-12-07 | 2012-06-07 | Sony Corporation | Method for evaluation of oxide semiconductor electrode, apparatus for evaluation of oxide semiconductor electrode, and apparatus for production of oxide semiconductor electrode |
| CN102156118A (en) * | 2011-03-22 | 2011-08-17 | 中国科学院长春应用化学研究所 | Method for detecting melamine |
| CN104098088A (en) * | 2014-06-17 | 2014-10-15 | 华南理工大学 | Preparation method for nano-zinc oxide modified graphene hybrid material |
| CN104941643A (en) * | 2015-06-16 | 2015-09-30 | 北京科技大学 | Preparation method of Ag-GQDs(Graphene Quantum Dots)/ZnO ternary photocatalyst |
| CN107321375A (en) * | 2017-07-13 | 2017-11-07 | 扬州工业职业技术学院 | A kind of SiO2/ZnO/g‑C3N4Nano material and its application in reduction of hexavalent chromium |
Non-Patent Citations (6)
| Title |
|---|
| XIANGFENG CHU ET AL.: "The acetic acid gas sensing properties of graphene quantum dots (GQDs)–ZnO nanocomposites prepared by hydrothermal method", 《J MATER SCI: MATER ELECTRON》 * |
| YING LI ET AL.: "Chemical sensors and biosensors for the detection of melamine", 《RSC ADVANCES》 * |
| 吴世敏 等: "《简明精细化工大辞典》", 30 June 1996, 辽宁科学技术出版社 * |
| 张俊: "石墨烯（量子点）—锌锡氧化物复合材料气敏性能研究", 《中国优秀硕士论文全文数据库 工程科技I辑》 * |
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