CN109298116A - A kind of detection method of nauclea officinalis medicinal extract piece - Google Patents
A kind of detection method of nauclea officinalis medicinal extract piece Download PDFInfo
- Publication number
- CN109298116A CN109298116A CN201811399693.0A CN201811399693A CN109298116A CN 109298116 A CN109298116 A CN 109298116A CN 201811399693 A CN201811399693 A CN 201811399693A CN 109298116 A CN109298116 A CN 109298116A
- Authority
- CN
- China
- Prior art keywords
- medicinal extract
- nauclea officinalis
- officinalis medicinal
- extract piece
- detection method
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/62—Detectors specially adapted therefor
- G01N30/74—Optical detectors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/86—Signal analysis
- G01N30/8675—Evaluation, i.e. decoding of the signal into analytical information
- G01N30/8686—Fingerprinting, e.g. without prior knowledge of the sample components
Landscapes
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Library & Information Science (AREA)
- Engineering & Computer Science (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
The invention belongs to pharmaceutical preparation detection fields, disclose a kind of detection method of nauclea officinalis medicinal extract piece, take nauclea officinalis medicinal extract piece to be measured that test solution is made, using high effective liquid chromatography for measuring finger-print, similarity-rough set is carried out with the standard finger-print of nauclea officinalis medicinal extract piece, the Quality estimation of nauclea officinalis medicinal extract piece is carried out according to similarity size.Compared with prior art, detection method is more simple and fast, stablize, precision is high, more there is specific aim to targeting active material, monitoring to product and evaluation more comprehensively, avoid and measure the chemical component at one, two peak only to determine the one-sidedness of total quality, stronger to the true and false sense of product.The present invention passes through the comparison of test sample map and standard diagram, and calculating takes similarity to be greater than 0.900, can effectively distinguish the nauclea officinalis medicinal extract piece true and false, evaluates nauclea officinalis medicinal extract piece superiority and inferiority, guarantees the consistency and validity of the kind.
Description
Technical field
The invention belongs to pharmaceutical preparation detection fields, and in particular to a kind of detection method of nauclea officinalis medicinal extract piece.
Background technique
Nauclea officinalis is subordinate to Rubiaceae Nauclea, and xylophyta is the famous southern medicine in China.Its character be yellow or brown color,
Crust yellowish-brown, it is coarse, it easily peels off, skin zone's section sepia or brown color, fibroid, matter is hard, and branch and bark are used as medicine, nothing
Smelly, bitter is cold in nature, return lung, large intestine, gallbladder, bladder meridian.With clearing heat and detoxicating, swelling and pain relieving and other effects, it is usually used in cold, fever,
Bronchitis, pneumonia, acute tonsillitis, sphagitis, mazoitis, cholecystitis, enteritis, bacillary dysentery, urinary tract infections, the ulcer of lower limb,
Ringworm of the foot infection, infection of burn, furuncle, eczema.
Nauclea officinalis medicinal extract piece is Chinese materia medica preparation made of being developed as nauclea officinalis single medicinal material, and it is acute that clinic, which is usually used in curing mainly,
Tonsillitis, acpuei pharyngitis, acute conjunctivitis and the infection of the upper respiratory tract.
For nauclea officinalis medicinal extract piece in existing state quality standard, detection method is relatively simple, and content is mainly with general flavone
Or based on alkaloid；Identifying is mainly thin-layer chromatography, only reduces accuracy and specificity by single chemical component identification,
It is unable to authenticity and consistency that effectively evaluating identifies nauclea officinalis medicinal extract piece, cannot reflect its inherent quality, not can solve adulterant
Or the problems such as substandard products doping.
Summary of the invention
In view of this, providing a kind of effective, reliable nauclea officinalis it is an object of the invention in view of the problems of the existing technology
The detection method of medicinal extract piece.
To achieve the purpose of the present invention, the present invention adopts the following technical scheme:
A kind of detection method of nauclea officinalis medicinal extract piece, takes nauclea officinalis medicinal extract piece to be measured that test solution is made, using efficient liquid phase
Chromatography determination finger-print carries out similarity-rough set with the standard finger-print of nauclea officinalis medicinal extract piece, according to similarity size into
The Quality estimation of row nauclea officinalis medicinal extract piece.
The detection method of nauclea officinalis medicinal extract piece of the present invention, using isovincoside lactone as the control of assay
Product establish the standard finger-print of nauclea officinalis medicinal extract piece, the standard finger-print according to product to be tested finger-print and nauclea officinalis medicinal extract piece
Compare and nauclea officinalis medicinal extract piece is detected.It is a kind of quantifiable chemical identification means of synthesis to finger-print, by feature
The appearance time at peak, peak area and relative scale analysis, reflect the chemical species of Chinese medicine and the characteristic of quantity,
Wherein, it is that nauclea officinalis medicinal extract piece to be measured is ground into powder that the specific method of test solution, which is made, in the nauclea officinalis medicinal extract piece to be measured
Membrane filtration after methanol dissolution is added in end.
Preferably, the mass volume ratio of the nauclea officinalis medicinal extract piece powder and methanol is 1:250 based on g/mL.
Preferably, the specific method of the methanol dissolution is power 150W, frequency 40kHz ultrasonication 30min.
Preferably, the filter sizes are 0.45 μm.
In some embodiments, 10 times of methanol dilutions are further included the steps that before the membrane filtration.
Preferably, the condition of high effective liquid chromatography for measuring finger-print described in detection method of the present invention is
Use octadecylsilane chemically bonded silica for chromatography column packing, with acetonitrile: water: phosphoric acid volume ratio is that 30:70:0.1 solution is
Mobile phase, Detection wavelength 226nm, flow velocity 1.0ml/min, sampling volume are 20 μ l, and column temperature is room temperature, and retention time is
70min, separating degree should be greater than 1.5, and 5000 should be not less than by calculating the theoretical number of plates by isovincoside lactone peak.
Preferably, each chromatographic peak in the standard finger-print of nauclea officinalis medicinal extract piece described in detection method of the present invention
Appearance time be followed successively by 5.12min, 8.95min, 14.35min, 19.25min, 28.33min, 36.56min, 39.88min,
42.55min,48.24min,59.34min,65.01min；The peak area of each chromatographic peak is followed successively by 145.33,301.12,
801.45、544.35、272.25、150.14、121.32、581.25、215.77、168.25、6026.14。
Preferably, similarity-rough set method described in detection method of the present invention is that test sample is obtained fingerprint image
Modal data imports Chinese Pharmacopoeia Commission's A editions " similarity evaluations " neutralization nauclea officinalis leachings in 2004
The standard finger-print of cream piece compares.
The standard of Quality estimation described in detection method of the present invention is similarity > 0.9, i.e., nauclea officinalis medicinal extract piece to be measured
Finger-print and nauclea officinalis medicinal extract piece standard finger-print similarity > 0.9 be used as nauclea officinalis medicinal extract piece clearance foundation.
As shown from the above technical solution, the present invention provides a kind of detection methods of nauclea officinalis medicinal extract piece, and nauclea officinalis to be measured is taken to soak
Test solution is made in cream piece, the standard finger-print using high effective liquid chromatography for measuring finger-print, with nauclea officinalis medicinal extract piece
Similarity-rough set is carried out, the Quality estimation of nauclea officinalis medicinal extract piece is carried out according to similarity size.Compared with prior art, of the invention
Using isovincoside lactone as the reference substance of assay, the standard finger-print of nauclea officinalis medicinal extract piece is established, according to be measured
Product finger-print detects nauclea officinalis medicinal extract piece compared with the standard finger-print of nauclea officinalis medicinal extract piece, and detection method is more simple
It is single quick, stablize, precision is high, more has specific aim to targeting active material；The standard fingerprint figure of nauclea officinalis medicinal extract piece
The effective quality for guaranteeing final products of the foundation of spectrum is stablized, and unanimously, monitoring and evaluation to product are more comprehensively avoided and only surveyed
The chemical component at fixed one, two peak determines the one-sidedness of total quality, stronger to the true and false sense of product.The present invention passes through
The comparison of test sample map and standard diagram, calculating take similarity to be greater than 0.900, can effectively distinguish that nauclea officinalis medicinal extract piece is true
Puppet evaluates nauclea officinalis medicinal extract piece superiority and inferiority, guarantees the consistency and validity of the kind.And subsequent detection only needs the mark with acquisition
Quasi- finger-print is compared, and no longer needs to each self manufacture standard finger-print.
Specific embodiment
The invention discloses a kind of detection methods of nauclea officinalis medicinal extract piece.Those skilled in the art can use for reference present disclosure,
It is suitably modified realization of process parameters.In particular, it should be pointed out that all similar substitutions and modifications carry out those skilled in the art
Say it is it will be apparent that they are considered as being included in the present invention.Method and product of the invention has passed through preferred embodiment
It is described, related personnel can obviously not depart from the content of present invention, carried out in spirit and scope to method described herein
Change or appropriate changes and combinations, carry out implementation and application the technology of the present invention.
For a further understanding of the present invention, below in conjunction with the embodiment of the present invention, to the technical side in the embodiment of the present invention
Case is clearly and completely described, it is clear that and described embodiments are only a part of the embodiments of the present invention, rather than all
Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art institute without making creative work
The every other embodiment obtained, shall fall within the protection scope of the present invention.
Unless otherwise specified, reagent involved in the embodiment of the present invention is commercial product, can pass through business canal
Road purchase obtains.
Embodiment 1, a kind of detection method of nauclea officinalis medicinal extract piece
Comparison liquid preparation: 80 DEG C of isovincoside lactones for being dried under reduced pressure 3h of phosphorus pentoxide of learning from else's experience are appropriate, and precision claims
It is fixed, add methanol that the solution of every 1ml 0.1mg containing isovincoside lactone is made, shakes up to get stock solution；Precision measures deposit
Liquid 1ml, 2ml, 3ml, 4ml, 5ml, are respectively placed in 25ml volumetric flask plus methanol dilution is to scale, shake up.
Test sample preparation: nauclea officinalis medicinal extract piece 10 is taken, is ground into a powder, precision weighs 0.2g, is placed in stuffed conical flask, adds
Enter 50ml methanol, weighed weight is ultrasonically treated (power 150W, frequency 40kHz) 30min, lets cool again weighed weight, mended with methanol
The weight of sufficient less loss, shakes up, and filtration takes filtrate 5ml to set in the volumetric flask of 50ml, and with methanol dilution scale, shakes up, with 0.45
μm filter membrane filter to get test solution.
Chromatographic condition: the silent winged U3000 series chromatograph of match, containing double ternary high-pressure pumps, autosampler, column oven, DAD inspection
Device is surveyed, chromatographic column uses octadecylsilane chemically bonded silica for filler (C18), WondaCract ODS-2 (250mm ×
4.6mm, partial size are 5 μm).Acetonitrile-water-phosphoric acid (30:70:0.1) is mobile phase；Detection wavelength is 226nm；Flow velocity is 1.0ml/
min；Sampling volume is 20 μ l；Column temperature is room temperature, retention time 70min.
Applicability inspection: separating degree should be greater than 1.5, calculate C by isovincoside lactone peak26H30N2O8It calculates, it is theoretical
The number of plates should be not less than 5000.
Embodiment 2, linear relationship are investigated
The 20 μ l of comparison liquid of above-mentioned various concentration is taken to inject high performance liquid chromatograph, using the concentration of reference substance as abscissa,
Peak area is ordinate, draws standard curve, obtains regression equation Y=3.12 × 10-3X+4.86×10-2, r=0.9998, in good
Good linear relationship.
Embodiment 3, precision test
Take " linear relationship investigate item under concentration be 12 μ g/ml reference substance solutions, continuous sample introduction 5 times, measure different catharanthus roseus
Glycosides lactams peak area, as a result RSD is 0.14%, shows that instrument precision is good.
Embodiment 4, repetitive test
6 parts of test sample are taken, prepares test solution by 1 the method for embodiment, and carry out assay, the results showed that should
Method repeatability is good (table 1).
The repeated data of table 1
Embodiment 5, stability test
A test solution is taken, respectively in 0h, 2h, 4h, 8h, 16h, measures the peak face of isovincoside lactone for 24 hours
Product, the results showed that good (table 2) in internal stability for 24 hours.
2 stability data of table
Embodiment 6, mark-on reclaims test
Same a collection of 6 parts of powder of the nauclea officinalis medicinal extract piece of known isovincoside lactone content is taken, respectively about 0.2g, is placed in tool plug
In conical flask, it is separately added into a certain amount of different Changchun China glycosides lactams comparison liquid, it must be for according to above-mentioned sample solution preparation method
Test sample solution carries out assay.The wherein calculation formula of recovery of standard addition are as follows: recovery of standard addition=(measured amount-sample contains
Amount)/scalar quantity * 100%.
3 recovery of standard addition data of table
The results show that average recovery rate is 97.87%, RSD 1.49%, show that the method has good recycling
Rate.
Test sample assay: it takes 6 batches of nauclea officinalis medicinal extract pieces to carry out assay according to the above method, as a result measures average content
For 10.82mg/ piece.
Embodiment 7, the high performance liquid chromatograph finger-print for establishing nauclea officinalis medicinal extract piece.
Finger-print: 20 batch of nauclea officinalis medicinal extract piece test sample is constructed, it is molten that test sample is made according to 1 the method for embodiment
Liquid adds methanol that the solution of every 1ml 1.0mg containing isovincoside lactone is made as reference substance.Take above-mentioned test sample molten respectively
Liquid and each 20 μ l of reference substance solution inject high performance liquid chromatograph according to the chromatographic condition of embodiment 1, obtain the confession of 20 batches
The finger-print of test sample solution finger-print and isovincoside lactone reference substance reference peak.
The determination at shared peak: according to the testing result of the test sample of 20 batch nauclea officinalis medicinal extract pieces, compare chromatogram, selection figure
The chromatographic peak that peak area is larger in spectrum, peak type is preferably relatively stable, appearance time is more consistent is used as shared peak, wherein peak area
It is maximum consistent the most referring to peak with isovincoside lactone reference substance appearance time.
The retention time at shared peak and the retention time referring to peak: 20 batch nauclea officinalis medicinal extract piece chromatographies are obtained according to map and are protected
The time is stayed as a result, calculating on the basis of referring to peak retention time, obtains each shared peak retention time and referring to peak retention time
Ratio, it is substantially stationary referring to peak relative retention time ratio, meet the requirement of finger-print.
The peak area at shared peak and the ratio referring to peak: obtaining 20 batch nauclea officinalis medicinal extract piece peak areas according to map as a result,
It is calculated on the basis of referring to peak peak area, obtains each shared peak peak area and the ratio referring to peak peak area, referring to peak with respect to peak
Area ratio is substantially stationary, meets the requirement of finger-print.
It establishes standard finger-print: the nauclea officinalis medicinal extract built-in testing data of 20 batches is imported into Chinese Pharmacopoeia Commission 2004
It is fitted data processing in A editions " similarity evaluations ", the standard for obtaining nauclea officinalis medicinal extract piece refers to
Line map (is shown in Table 4).And the finger-print of this 20 batch and the similarity of standard diagram are all larger than 0.900.
Table 4 shares peak retention time and peak area information
Note: No. 11 peaks are isovincoside lactone referring to peak.
Determining fingerprint pattern method: measurement test sample obtains finger-print data, imports Chinese Pharmacopoeia Commission 2004 A editions
" similarity evaluation " neutralizes the standard finger-print having had been built up and compares, and passes through similarity
It calculates and can be used as nauclea officinalis medicinal extract piece clearance foundation greater than 0.900.Subsequent detection only need with obtain standard finger-print into
Row compares, and no longer needs to each self manufacture standard finger-print.
Claims (9)
1. a kind of detection method of nauclea officinalis medicinal extract piece, takes nauclea officinalis medicinal extract piece to be measured that test solution is made, using high-efficient liquid phase color
Spectrometry measures finger-print, carries out similarity-rough set with the standard finger-print of nauclea officinalis medicinal extract piece, carries out according to similarity size
The Quality estimation of nauclea officinalis medicinal extract piece.
2. detection method according to claim 1, the specific method of test solution is made in the nauclea officinalis medicinal extract piece to be measured
Membrane filtration after methanol dissolution is added is ground into a powder for nauclea officinalis medicinal extract piece to be measured.
3. detection method according to claim 2, based on g/mL, the quality volume of the nauclea officinalis medicinal extract piece powder and methanol
Than for 1:250；The specific method of the methanol dissolution is power 150W, frequency 40kHz ultrasonication 30min；The filter membrane
Aperture is 0.45 μm.
4. detection method according to claim 2 or 3,10 times of methanol dilutions are further included the steps that before the membrane filtration.
5. detection method according to claim 1, the condition of the high effective liquid chromatography for measuring finger-print is to use
Octadecylsilane chemically bonded silica is chromatography column packing, and using acetonitrile: water: phosphoric acid volume ratio is 30:70:0.1 solution as flowing
Phase, Detection wavelength 226nm, flow velocity 1.0ml/min, sampling volume are 20 μ l, and column temperature is room temperature, retention time 70min,
Separating degree should be greater than 1.5, and 5000 should be not less than by calculating the theoretical number of plates by isovincoside lactone peak.
6. detection method according to claim 1, the high-efficient liquid phase chromatogram process measuring method is that 20 μ l of test sample is taken to infuse
Enter high performance liquid chromatograph detection.
7. detection method according to claim 1, each chromatographic peak goes out in the standard finger-print of the nauclea officinalis medicinal extract piece
Peak time be followed successively by 5.12min, 8.95min, 14.35min, 19.25min, 28.33min, 36.56min, 39.88min,
42.55min,48.24min,59.34min,65.01min；The peak area of each chromatographic peak is followed successively by 145.33,301.12,
801.45、544.35、272.25、150.14、121.32、581.25、215.77、168.25、6026.14。
8. detection method according to claim 1, the similarity-rough set method is that test sample is obtained finger-print number
According to importing Chinese Pharmacopoeia Commission A editions " similarity evaluation " neutralization nauclea officinalis medicinal extract pieces in 2004
Standard finger-print compare.
9. detection method according to claim 1, the standard of the Quality estimation is similarity > 0.9.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811399693.0A CN109298116A (en) | 2018-11-22 | 2018-11-22 | A kind of detection method of nauclea officinalis medicinal extract piece |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811399693.0A CN109298116A (en) | 2018-11-22 | 2018-11-22 | A kind of detection method of nauclea officinalis medicinal extract piece |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109298116A true CN109298116A (en) | 2019-02-01 |
Family
ID=65143779
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811399693.0A Pending CN109298116A (en) | 2018-11-22 | 2018-11-22 | A kind of detection method of nauclea officinalis medicinal extract piece |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109298116A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111198244A (en) * | 2020-01-15 | 2020-05-26 | 海南森祺制药有限公司 | Thin-layer chromatography detection method for nauclea officinalis extract syrup |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1958002A (en) * | 2005-10-31 | 2007-05-09 | 海南制药厂有限公司 | Intermediate of fathead tree medicinal materials and quality control method of injection |
| CN103185714A (en) * | 2011-12-29 | 2013-07-03 | 贾晓斌 | Quality control method of nauclea officinalis Pierrc ex Pitard preparation with heat-clearing and detoxicating, antibacterial and anti-inflammatory efficacy |
| CN106074788A (en) * | 2016-06-29 | 2016-11-09 | 中国医学科学院药用植物研究所海南分所 | A kind of Lignum et Radix Naucleae total alkaloids extract and preparation method and application |
| CN108785416A (en) * | 2018-08-29 | 2018-11-13 | 海南森祺制药有限公司 | A kind of Lignum et Radix Naucleae extract lozenge and preparation method |
-
2018
- 2018-11-22 CN CN201811399693.0A patent/CN109298116A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1958002A (en) * | 2005-10-31 | 2007-05-09 | 海南制药厂有限公司 | Intermediate of fathead tree medicinal materials and quality control method of injection |
| CN103185714A (en) * | 2011-12-29 | 2013-07-03 | 贾晓斌 | Quality control method of nauclea officinalis Pierrc ex Pitard preparation with heat-clearing and detoxicating, antibacterial and anti-inflammatory efficacy |
| CN106074788A (en) * | 2016-06-29 | 2016-11-09 | 中国医学科学院药用植物研究所海南分所 | A kind of Lignum et Radix Naucleae total alkaloids extract and preparation method and application |
| CN108785416A (en) * | 2018-08-29 | 2018-11-13 | 海南森祺制药有限公司 | A kind of Lignum et Radix Naucleae extract lozenge and preparation method |
Non-Patent Citations (5)
| Title |
|---|
| KUI SU: "Study on Chemical Composition of Nauclea Officinalis Leaves", 《INTERNATIONAL JOURNAL OF CHEMISTRY》 * |
| 丁琳 等: "HPLC法测定胆木注射液中异长春花苷内酰胺的含量", 《中医药学刊》 * |
| 国家食品药品监督管理局: "《国家药品标准 YBZ13472006-2009Z》", 9 April 2009 * |
| 王月 等: "胆木及其制剂研究进展", 《亚太传统医药》 * |
| 黄有兴 等: "HPLC法测定胆木浸膏胶囊中异长春花苷内酰胺的含量", 《中国热带医学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111198244A (en) * | 2020-01-15 | 2020-05-26 | 海南森祺制药有限公司 | Thin-layer chromatography detection method for nauclea officinalis extract syrup |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102138985B (en) | Quality control method of total glycosides single preparation of white paeony roots | |
| CN103364506A (en) | Quality control method for effective parts of pomelo flavedo and pomelo flavedo preparation | |
| CN106198782A (en) | A kind of can realize the content analysis of 18 components in Folium Ilicis and the method for quality control of similarity evaluation simultaneously | |
| CN103869003A (en) | Establishing method of double-solvent fused HPLC fingerprint of medicinal phellodendron and standard fingerprint of medicinal phellodendron | |
| CN107796892A (en) | The finger-print of Macrophylla dragon capsule and its application in quality control and constituent analysis | |
| CN107356691A (en) | Build the detection method of bent finger-print | |
| CN105372350B (en) | A kind of low-sugar type intensified loquet distillate fingerprint control method | |
| CN109374789A (en) | The construction method and detection method of Cortex Phellodendri medicinal material HPLC characteristic spectrum | |
| CN104316613B (en) | A kind of method for building up of dispelling wind detoxicating capsule finger printing | |
| CN102650626A (en) | Standard fingerprint of fleabane phenol bulk drug and preparation, establishment method and application | |
| CN108872410A (en) | A kind of method for building up and its finger-print of lung-nourishing semifluid extract finger-print | |
| CN105223300A (en) | The method of quality control of lichee polyphenol and products thereof | |
| CN103969350A (en) | Establishment method of HPLC fingerprint of andrographispaniculata and indian stringbush root anti-inflammatory capsule | |
| CN110243988A (en) | The method for building up of strong medicine white flower nine inner bright HPLC finger-print | |
| CN110031564A (en) | The quality determining method of natural plants anticoccidial feed addictive based on HPLC finger-print | |
| CN109298116A (en) | A kind of detection method of nauclea officinalis medicinal extract piece | |
| CN109324141A (en) | The characteristic fingerprint pattern and its construction method of a kind of wide dragon or its processed product and application | |
| CN103575821A (en) | Detection method of 14 chemical components in Tangminling preparation | |
| CN104849375A (en) | Method for detecting exocarpium citrus grandis Tanke compound | |
| CN102552515A (en) | Quality detection method for blood-nourishing Chinese angelica syrup | |
| CN102323343B (en) | Method for establishing Pericarpium Juglantis high-performance liquid chromatography (HPLC) fingerprint chromatogram and application method thereof | |
| CN105954311A (en) | Method for constructing <1>H-NMR fingerprint spectra of Periplaneta americana and extract of Periplaneta americana | |
| CN103926350A (en) | Inspection method of rehabilitation liquid formulation fingerprint and standard fingerprint | |
| CN109596744A (en) | A kind of HPLC detection method of Chinese materia medica preparation | |
| CN113607855B (en) | Fingerprint spectrum of medicinal preparation of snakegourd fruit, allium macrostemon and pinellia ternata decoction, and establishing method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190201 |