CN109298100A - A kind of metabonomic analysis methods of Cultures of S. cerevisiae - Google Patents
A kind of metabonomic analysis methods of Cultures of S. cerevisiae Download PDFInfo
- Publication number
- CN109298100A CN109298100A CN201811433493.2A CN201811433493A CN109298100A CN 109298100 A CN109298100 A CN 109298100A CN 201811433493 A CN201811433493 A CN 201811433493A CN 109298100 A CN109298100 A CN 109298100A
- Authority
- CN
- China
- Prior art keywords
- cultures
- cerevisiae
- analysis methods
- metabonomic analysis
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
Abstract
The present invention relates to technical field of analytical chemistry, more particularly to a kind of metabonomic analysis methods of Cultures of S. cerevisiae, using the metabolism group ingredient of the method measurement yeast culture of GC/MS derivatization.Sample pre-treatments are carried out using silane derivatization.Design innovation of the present invention, establish the metabolism group ingredient of the method measurement yeast culture of GC/MS derivatization, sample pre-treatments are carried out using silane derivatization, institute's construction method can detect main metabolic species in yeast culture such as sugar, amino acid, alcohol, organic acid, alkane.The method is suitable for the metabolism group research of yeast culture, basis can be provided for its effective substance group i.e. research of its biomarker, and provide reference for the research and development of probiotics and development microbial metabolism group correlative study.
Description
Technical field
The present invention relates to analytical chemistry fields, more particularly to a kind of metabonomic analysis side of Cultures of S. cerevisiae
Method.
Background technique
Yeast culture is full of nutrition, complicated component, mainly most of by yeast metabolism product, viable yeast bacterium, culture medium 3
Composition.Many years scientific research and production application are it was verified that yeast culture is to adjust animal intestines and stomach by its metabolite
The quantity and balance of microbiota in road to ensure animal health and improve breeding performonce fo animals with this.However wherein institute
The metabolite for including is many kinds of, and in addition to the nutriment known to the minority, majority is with peace existing for various compound forms
The complete non-principal component of property, does not have this and definitely describes and explains, and impossible each ingredient be yeast culture it is effective at
Point, while also can be considered as its main or specific action component without any metabolite.Therefore yeast training
It supports which the main active that functions in object ingredient has, has how what kind of acts synergistically between them, be desirable
Further the problem of research confirmation.
Summary of the invention
It is an object of the present invention to overcome the above-mentioned technical problems, and to provide the metabolism group of a kind of Cultures of S. cerevisiae point
Analysis method can be fully solved above-mentioned technical problem.
The technical solution for solving above-mentioned technical problem is as follows:
A kind of metabonomic analysis methods of Cultures of S. cerevisiae are the method measurement yeast using GC/MS derivatization
The metabolism group ingredient of culture.
Before measurement, sample pre-treatments are carried out using silane derivatization.
Specifically includes the following steps:
(1) Cultures of S. cerevisiae is divided into five kinds according to fermentation time, be respectively as follows: A-12h, B-24h, C-36h, D-48h,
E-60h；Five kinds of each 50mL of Cultures of S. cerevisiae are taken, then 50mL culture medium is taken to be denoted as F- culture medium；It is desk-top to be placed in MIKRO-22R
Freeze-drying 48h is carried out in refrigerated centrifuge；
(2) after the completion of being lyophilized, six groups of samples respectively take 0.05g；Da Nongwei Yikang VXP powder sample 0.05g is weighed again, is denoted as
G-VXP；
(3) above-mentioned seven groups of samples are respectively placed in 5ml PE pipe, every group of 20mg/mL methoxamine hydrochloric acid for being added 100 μ L
Pyridine solution is placed in ultrasonic wave and shakes 30s, in 37 DEG C of oximation reaction 90min；
(4) it is taken out out of ultrasonic wave, the derivative reagent of the BSTFA (containing 1%TMCS) of every group of 200 μ L of addition is anti-in 70 DEG C
Answer 60min；
(5) every group of sample by step (4) after derivative is centrifuged 10min in 10000r/min, takes supernatant each
50vl is added n-hexane and quantitatively sets 0.5ml, is directly injected into gas chromatography-mass spectrometry instrument and analyzed；Obtaining TIC figure
Identification identification is carried out to each chromatographic peak ingredient afterwards；
Wherein, GC conditions are as follows: chromatographic column is HP-5MS (30m × 0.25mm × 0.25 μm) elastic quartz capillary tube
Column, 80 DEG C of column temperature (retain 3min), are warming up to 150 DEG C with 5 DEG C/min, keep 10min；280 DEG C are warming up to 10 DEG C/min, is protected
Hold 10min；Carrier gas is high-purity He (99.999%)；7.62psi, carrier gas flux 1.0mL/min are pressed before column；1 μ L of sample volume；It shunts
Than 10:1, solvent delay time 3min；
Mass Spectrometry Conditions are as follows: ion source is the source EI；230 DEG C of ion source temperature；150 DEG C of quadrupole rod temperature；Electron energy 70eV；It connects
280 DEG C of temperature of mouth；20~800amu of mass scan range.
It further says, the data prediction application software MSD of gas chromatography-mass spectrography described in step (5)
Chem station (E.02.01.1177, Agilent), including identify chemical combination according to retention time and quota ion pair and look for
Spectral peak carries out integral and carries out Data correction to integral result；Then pass through Xcalibur1.2 version software and standard spectra count
Metabolin is pointed out according to library NIST11 retrieval matching result, authenticating compound.
After compound identification, it is soft that metabolin title and chromatographic peak area the correction result of identification are imported into SIMCA.P+13.0
Part (Umetrics AB,Sweden), right using principal component analysis (principalcomponent analysis, PCA)
Sample distribution trend carries out PCA analysis.
The beneficial effects of the present invention are:
Design innovation of the present invention establishes the metabolism group ingredient of the method measurement yeast culture of GC/MS derivatization, uses
Silane derivatization carry out sample pre-treatments, institute's construction method to main metabolic species in yeast culture such as sugar, amino acid, alcohol,
Organic acid, alkane etc. can detect.The method is suitable for the metabolism group research of yeast culture, can for its effective substance group i.e. its
The research of biomarker provides basis, and provides for the research and development of probiotics and development microbial metabolism group correlative study
With reference to.
Detailed description of the invention
The present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.
Fig. 1 is the GC/MS total ion chromatogram (TIC) of seven kinds of samples of the invention；
Fig. 2 is the PCA scatterplot X-Y scheme of seven kinds of samples of the invention.
Specific embodiment
Embodiment 1:
Select material:
Bis- (trimethyl silane) trifluoroacetamides of N, O- (BSTFA contains 1%TMCS), (U.S. trim,ethylchlorosilane TMCS
Sigma-Aldrich company), pyridine (chromatographically pure, Shanghai Aladdin biochemical technology limited liability company), the n-hexane (U.S.
TEDIA company), methoxamine hydrochloride (Sigma-Aldrich).
Agilent7890A/5975C gas chromatograph-mass spectrometer (GC-MS) (Agilent company of the U.S.), MIKRO-22R is desk-top
Cryogenic freezing centrifuge (German Hettich company).
Analysis method specific steps are as follows:
(1) Cultures of S. cerevisiae is divided into five kinds according to fermentation time, be respectively as follows: A-12h, B-24h, C-36h, D-48h,
E-60h；Five kinds of each 50mL of Cultures of S. cerevisiae are taken, then 50mL culture medium is taken to be denoted as F- culture medium；It is desk-top to be placed in MIKRO-22R
Freeze-drying 48h is carried out in refrigerated centrifuge；
(2) after the completion of being lyophilized, six groups of samples respectively take 0.05g；Da Nongwei Yikang VXP powder sample 0.05g is weighed again；It is described
Da Nongwei Yikang VXP powder sample be the yeast culture for originating from Da Nongwei company of the U.S.；As object of reference, it is denoted as G-VXP；
(3) above-mentioned seven groups of samples are respectively placed in 5ml PE pipe, every group of 20mg/mL methoxamine hydrochloric acid for being added 100 μ L
Pyridine solution is placed in ultrasonic wave and shakes 30s, in 37 DEG C of oximation reaction 90min；
(4) it is taken out out of ultrasonic wave, the derivative reagent of the BSTFA (containing 1%TMCS) of every group of 200 μ L of addition is anti-in 70 DEG C
Answer 60min；
(5) every group of sample by step (4) after derivative is centrifuged 10min in 10000r/min, takes supernatant each
50vl is added n-hexane and quantitatively sets 0.5ml, is directly injected into gas chromatography-mass spectrometry instrument and analyzed；Obtaining TIC figure
Identification identification is carried out to each chromatographic peak ingredient afterwards；
Wherein, GC conditions are as follows: chromatographic column is HP-5MS (30m × 0.25mm × 0.25 μm) elastic quartz capillary tube
Column, 80 DEG C of column temperature (retain 3min), are warming up to 150 DEG C with 5 DEG C/min, keep 10min；280 DEG C are warming up to 10 DEG C/min, is protected
Hold 10min；Carrier gas is high-purity He (99.999%)；7.62psi, carrier gas flux 1.0mL/min are pressed before column；1 μ L of sample volume；It shunts
Than 10:1, solvent delay time 3min；
Mass Spectrometry Conditions are as follows: ion source is the source EI；230 DEG C of ion source temperature；150 DEG C of quadrupole rod temperature；Electron energy 70eV；It connects
280 DEG C of temperature of mouth；20~800amu of mass scan range.
The data prediction application software MSD Chem station of the gas chromatography-mass spectrography
(E.02.01.1177, Agilent), including identify compound chromatographic peak according to retention time and quota ion pair and integrated
And Data correction is carried out to integral result；Then it is examined by Xcalibur1.2 version software and standard mass spectrometry database NIST11
Rope matching result points out metabolin, authenticating compound.
In the embodiment, the analysis of GC/MS full scan, the result of yeast culture Metabolite analysis are carried out to seven groups of samples
It is as follows, the total ion of GC/MS of the yeast culture at each time point, yeast culture culture medium and Da Nongwei Yikang VXP product
Flow chromatography figure is as shown in Fig. 1.
By each peak of sample chromatogram figure after scanning of the mass spectrum, the parsing of gas chromatography-mass spectrum data is carried out, has detected 97 kinds
Compound, relatively really by NIST11 mass spectrometric data system retrieval, verification standard mass spectrogram and to base peak, relative abundance etc.
Recognize, identify 85 kinds of compounds altogether, the relative amount of each component is quantified using areas of peak normalization method, and each sample metabolism produces
The Components identification result of object is as shown in table 1 below.
1 seven kinds of sample metabolite identifications of table
Note: N/A indicates that the substance is not present in table.
After compound identification, it is soft that metabolin title and chromatographic peak area the correction result of identification are imported into SIMCA.P+13.0
Part (Umetrics AB,Sweden), using principal component analysis (principal component analysis, PCA)
PCA analysis is carried out to sample distribution trend；Scatter plot is as shown in attached drawing 2.
It is shown by Fig. 2, its metabolite of the yeast culture of differentiation culture is found out on (in PC1 axis) by result in gradually
The trend of variation, ABCDE group sample are closer, and illustrate that the metabolite composition of this 5 fermentation stages is close, but there are still
Different, culture medium F group farther out, illustrate that its constituent is obviously different from the yeast of 5 fermentation stages with remaining 5 groups distances
Culture ingredient.And B and E group sample is then apart from each other, illustrates that this two groups of metabolite compositions are dramatically different.G-VXP product with
Remaining each group distance is farthest, and the yeast culture composition for illustrating that the composition of product is cultivated with the present invention differs greatly.
Yeast culture is mainly produced by the yeast cells metabolism formed after nutrients, cell wall and the fermentation in yeast cells
Object composition, wherein intracellular nutriment mainly has protein, nucleic acid, amino acid, small peptide, vitamin, minerals etc., cell
Wall main component is beta glucan, manna oligosacchride, and products of cellular metabolism is mainly that trophism metabolin is (polypeptide, oligosaccharides, organic
Acid), flavour enhancing substance (nucleotide, amino acid, polypeptide), aromatic substance (esters, alcohols), enzyme and other unknown growth factor.
Broken wall treatment has also been made in the yeast culture that the present embodiment ferments during the fermentation, therefore its main component composition is also divided
For three parts, as shown in Table 1, the yeast culture ingredient of the present embodiment differentiation culture is mainly amino acid, organic acid, ester
Class, alcohols, oligosaccharide kind etc., and it is previously reported consistent.These nutriments provide nutriment abundant for animal growth,
Gastrointestinal micro-flora balance is adjusted, utilization of nutrients is improved.In addition, the yeast culture of the present embodiment fermentation output
For product compared with G-VXP product, the two contains common substance, predominantly short chain fatty acids (propionic acid, acetic acid), amino acid (figured silk fabrics
Propylhomoserin, alanine, leucine, glycine, serine, proline, asparatate), urea, organic acid (succinic acid, rich horse
Acid, palmitinic acid), inositol, sucrose etc..
Design innovation of the present invention establishes the metabolism group ingredient of the method measurement yeast culture of GC/MS derivatization, uses
Silane derivatization carry out sample pre-treatments, institute's construction method to main metabolic species in yeast culture such as sugar, amino acid, alcohol,
Organic acid, alkane etc. can detect.The method is suitable for the metabolism group research of yeast culture, can for its effective substance group i.e. its
The research of biomarker provides basis, and provides for the research and development of probiotics and development microbial metabolism group correlative study
With reference to.
The above is only presently preferred embodiments of the present invention, not does limitation in any form to the present invention, it is all according to
According to any simple modification to the above embodiments in technical spirit of the invention, equivalent variations, guarantor of the invention is each fallen within
Within the scope of shield.
Claims (10)
1. a kind of metabonomic analysis methods of Cultures of S. cerevisiae, which is characterized in that surveyed using the method for GC/MS derivatization
Determine the metabolism group ingredient of yeast culture.
2. the metabonomic analysis methods of Cultures of S. cerevisiae according to claim 1, which is characterized in that use silane
Derivatization carries out sample pre-treatments.
3. the metabonomic analysis methods of described in any item Cultures of S. cerevisiae, feature exist according to claim 1~2
In, comprising the following steps:
(1) Cultures of S. cerevisiae is divided into five kinds according to fermentation time, is respectively as follows: A-12h, B-24h, C-36h, D-48h, E-
60h；Five kinds of each 50mL of Cultures of S. cerevisiae are taken, then 50mL culture medium is taken to be denoted as F- culture medium；It is placed in tabletop refrigerated centrifuge
In carry out freeze-drying 48h；
(2) after the completion of being lyophilized, six groups of samples respectively take 0.05g；Da Nongwei Yikang VXP powder sample 0.05g, VXP powder is weighed again
Sample is denoted as G-VXP as reference；
(3) above-mentioned seven groups of samples are respectively placed in 5ml PE pipe, every group of 20mg/mL methoxamine pyridine hydrochloride for being added 100 μ L
Solution is placed in ultrasonic wave and shakes 30s, in 37 DEG C of oximation reaction 90min；
(4) it is taken out out of ultrasonic wave, the derivative reagent of the BSTFA of every group of 200 μ L of addition, in 70 DEG C of reaction 60min；
(5) every group of sample by step (4) after derivative is centrifuged 10min in 10000r/min, takes each 50vl of supernatant, add
It is quantitative to 0.5ml to enter n-hexane, is directly injected into gas chromatography-mass spectrometry instrument and is analyzed；To colors after obtaining TIC figure
Spectral peak ingredient carries out identification identification；
Wherein, GC conditions are as follows: chromatographic column is HP-5MS (30m × 0.25mm × 0.25 μm) fused-silica capillary column,
80 DEG C of column temperature (retains 3min), is warming up to 150 DEG C with 5 DEG C/min, keeps 10min；280 DEG C are warming up to 10 DEG C/min, is kept
10min；Carrier gas is high-purity He (99.999%)；7.62psi, carrier gas flux 1.0mL/min are pressed before column；1 μ L of sample volume；Split ratio
10:1, solvent delay time 3min；
Mass Spectrometry Conditions are as follows: ion source is the source EI；230 DEG C of ion source temperature；150 DEG C of quadrupole rod temperature；Electron energy 70eV；Interface temperature
280 DEG C of degree；20~800amu of mass scan range.
4. the metabonomic analysis methods of Cultures of S. cerevisiae according to claim 3, which is characterized in that step (5)
Described in gas chromatography-mass spectrography data prediction application software MSD Chem station (E.02.01.1177,
Agilent), including according to retention time and quota ion pair identify compound chromatographic peak carry out integral and to integral result into
Row Data correction；Then matching result is retrieved to generation by Xcalibur1.2 version software and standard mass spectrometry database NIST11
It thanks to object to be pointed out, authenticating compound.
5. the metabonomic analysis methods of Cultures of S. cerevisiae according to claim 4, which is characterized in that further include:
After compound identification, metabolin title and chromatographic peak area the correction result of identification are imported into SIMCA.P+13.0 software
(Umetrics AB,Sweden), right using principal component analysis (principal component analysis, PCA)
Sample distribution trend is analyzed.
6. the metabonomic analysis methods of Cultures of S. cerevisiae according to claim 3, which is characterized in that step (1)
Described in tabletop refrigerated centrifuge be Hettich company of Germany the desk-top cryogenic freezing centrifuge of MIKRO-22R.
7. the metabonomic analysis methods of Cultures of S. cerevisiae according to claim 3, which is characterized in that step (1)
A-12h, B-24h, C-36h, D-48h, E-60h and F- culture medium is that Jilin Agriculture University Ji Nongborui milk cow is raised
Expect that research and development centre provides.
8. the metabonomic analysis methods of Cultures of S. cerevisiae according to claim 3, which is characterized in that step (3)
Described in pyridine be Shanghai Aladdin biochemical technology limited liability company chromatographically pure grade pyridine；Methoxamine hydrochloride is the U.S.
The methoxamine hydrochloride of Sigma-Aldrich company.
9. the metabonomic analysis methods of Cultures of S. cerevisiae according to claim 3, which is characterized in that step (4)
Described in BSTFA be the N containing 1%TMCS, bis- (trimethyl silane) trifluoroacetamides of O-；The TMCS is U.S. Sigma-
The trim,ethylchlorosilane of Aldrich.
10. the metabonomic analysis methods of Cultures of S. cerevisiae according to claim 3, which is characterized in that step (5)
Described in gas chromatography-mass spectrometry instrument be U.S. Agilent company Agilent7890A/5975C gas-chromatography-matter
Compose combined instrument；N-hexane is the n-hexane of U.S. TEDIA company.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811433493.2A CN109298100A (en) | 2018-11-28 | 2018-11-28 | A kind of metabonomic analysis methods of Cultures of S. cerevisiae |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811433493.2A CN109298100A (en) | 2018-11-28 | 2018-11-28 | A kind of metabonomic analysis methods of Cultures of S. cerevisiae |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109298100A true CN109298100A (en) | 2019-02-01 |
Family
ID=65143962
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811433493.2A Pending CN109298100A (en) | 2018-11-28 | 2018-11-28 | A kind of metabonomic analysis methods of Cultures of S. cerevisiae |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109298100A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110632227A (en) * | 2019-09-12 | 2019-12-31 | 中南林业科技大学 | Method for distinguishing Liuyang fermented soya beans, Yangjiang fermented soya beans and Yongchuan fermented soya beans |
| CN114720618A (en) * | 2022-03-30 | 2022-07-08 | 集美大学 | Analysis method of Phaffia yeast metabolite |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009134439A2 (en) * | 2008-05-02 | 2009-11-05 | Purdue Research Foundation | Group specific internal standard technology (gsist) for simultaneous identification and quantification of small molecules |
| CN101936971A (en) * | 2010-09-13 | 2011-01-05 | 天津大学 | Method for seeking biomarkers in penicillin fermentation process |
| CN103698451A (en) * | 2013-12-05 | 2014-04-02 | 柳州联海科技有限公司 | Method for detecting amino acid in fermenting liquid by using gas chromatography-mass spectrometry |
| CN103808841A (en) * | 2013-12-05 | 2014-05-21 | 陈东 | Method for detecting organic acid, amino acid and sugar in fermented liquid by gas chromatography-mass spectrometry |
| CN107561058A (en) * | 2017-09-12 | 2018-01-09 | 青岛啤酒股份有限公司 | A kind of industrial Lager yeast strains flavor substance metabolism method for evaluating similarity |
-
2018
- 2018-11-28 CN CN201811433493.2A patent/CN109298100A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009134439A2 (en) * | 2008-05-02 | 2009-11-05 | Purdue Research Foundation | Group specific internal standard technology (gsist) for simultaneous identification and quantification of small molecules |
| CN101936971A (en) * | 2010-09-13 | 2011-01-05 | 天津大学 | Method for seeking biomarkers in penicillin fermentation process |
| CN103698451A (en) * | 2013-12-05 | 2014-04-02 | 柳州联海科技有限公司 | Method for detecting amino acid in fermenting liquid by using gas chromatography-mass spectrometry |
| CN103808841A (en) * | 2013-12-05 | 2014-05-21 | 陈东 | Method for detecting organic acid, amino acid and sugar in fermented liquid by gas chromatography-mass spectrometry |
| CN107561058A (en) * | 2017-09-12 | 2018-01-09 | 青岛啤酒股份有限公司 | A kind of industrial Lager yeast strains flavor substance metabolism method for evaluating similarity |
Non-Patent Citations (5)
| Title |
|---|
| ELSUIDA KONDO 等: "Metabolic profiling of yeast culture using gas chromatography coupled with orthogonal acceleration accurate mass time-of-flight mass spectrometry: Application to biomarker discovery", 《ANALYTICA CHIMICA ACTA》 * |
| HIROKO KATO 等: "Widely targeted metabolic profiling analysis of yeast central metabolites", 《JOURNAL OF BIOSCIENCE AND BIOENGINEERING》 * |
| SEUNG-HO SEO 等: "Metabolite profiling of Makgeolli for the understanding of yeast fermentation characteristics during fermentation and aging", 《PROCESS BIOCHEMISTRY》 * |
| 李玉冬 等: "常见食源性致病菌胞外代谢轮廓分析", 《现代食品科技》 * |
| 韩晓江 等: "糖胁迫下鲁氏接合酵母的代谢指纹分析", 《食品科学》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110632227A (en) * | 2019-09-12 | 2019-12-31 | 中南林业科技大学 | Method for distinguishing Liuyang fermented soya beans, Yangjiang fermented soya beans and Yongchuan fermented soya beans |
| CN114720618A (en) * | 2022-03-30 | 2022-07-08 | 集美大学 | Analysis method of Phaffia yeast metabolite |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Campbell et al. | Methionine metabolism alters oxidative stress resistance via the pentose phosphate pathway | |
| Almstetter et al. | Comprehensive two-dimensional gas chromatography in metabolomics | |
| Xiong et al. | 13C-tracer and gas chromatography-mass spectrometry analyses reveal metabolic flux distribution in the oleaginous microalga Chlorella protothecoides | |
| Treves et al. | Carbon flux through photosynthesis and central carbon metabolism show distinct patterns between algae, C3 and C4 plants | |
| Peltier et al. | The Oligomeric Stromal Proteome of Arabidopsis thaliana Chloroplasts* S | |
| Gregg et al. | Subsistence practices and pottery use in Neolithic Jordan: molecular and isotopic evidence | |
| Wang et al. | Metabolomic and proteomic profiles reveal the dynamics of primary metabolism during seed development of lotus (Nelumbo nucifera) | |
| Berkov et al. | Development and validation of a GC–MS method for rapid determination of galanthamine in Leucojum aestivum and Narcissus ssp.: A metabolomic approach | |
| Lv et al. | Metabolomic profiling of the astaxanthin accumulation process induced by high light in Haematococcus pluvialis | |
| Zachleder et al. | Stable isotope compounds-production, detection, and application | |
| Gemperline et al. | Multifaceted investigation of metabolites during nitrogen fixation in Medicago via high resolution MALDI-MS imaging and ESI-MS | |
| Schwechheimer et al. | Towards better understanding of industrial cell factories: novel approaches for 13C metabolic flux analysis in complex nutrient environments | |
| Mairinger et al. | Gas chromatography-quadrupole time-of-flight mass spectrometry-based determination of isotopologue and tandem mass isotopomer fractions of primary metabolites for 13C-metabolic flux analysis | |
| Xue et al. | A mass spectrometry‐based high‐throughput screening method for engineering fatty acid synthases with improved production of medium‐chain fatty acids | |
| CN106053674A (en) | Chromatographic detection method for simultaneously analyzing ammonium ions, amino acids and biogenic amine in tobacco leaves | |
| CN102033114A (en) | Method for quickly and simply analyzing microbial community structure of yeast for traditional brewage | |
| Min et al. | In-depth investigation of low-abundance proteins in matured and filling stages seeds of Glycine max employing a combination of protamine sulfate precipitation and TMT-based quantitative proteomic analysis | |
| CN109298100A (en) | A kind of metabonomic analysis methods of Cultures of S. cerevisiae | |
| Chung et al. | Fatty acids and stable isotope ratios in shiitake mushrooms (Lentinula edodes) indicate the origin of the cultivation substrate used: A preliminary case study in Korea | |
| Puzanskiy et al. | Analysis of metabolic profile of Chlamydomonas reinhardtii cultivated under autotrophic conditions | |
| Liu et al. | Culture conditions and nutrition requirements for the mycelial growth of Isaria farinosa (Hypocreales: Cordycipitaceae) and the altitude effect on its growth and metabolome | |
| Ghatnur et al. | Culture conditions for production of biomass, adenosine, and cordycepin from Cordyceps sinensis CS1197: Optimization by desirability function method | |
| Lv et al. | Analysis of metabolic responses of Dunaliella salina to phosphorus deprivation | |
| Wang et al. | The effect of intracellular amino acids on GSH production by high-cell-density cultivation of Saccharomyces cerevisiae | |
| Khoomrung et al. | Expanded metabolite coverage of Saccharomyces cerevisiae extract through improved chloroform/methanol extraction and tert-butyldimethylsilyl derivatization |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information | ||
| CB02 | Change of applicant information |
Address after: 130000 Changchun Changjiang Road Economic Development Zone, Jilin Province Applicant after: Changchun Borui Technology Co.,Ltd. Address before: 130000 Section 161, floor 5, No. 57, Changjiang Road, Changchun Economic Development Zone, Jilin Province Applicant before: CHANGCHUN BORUI AGRICULTURE AND ANIMAL HUSBANDRY GROUP CO.,LTD. |