CN109294886A - A kind of scalable ultrasonic cell disruption instrument probe with automatic detection function - Google Patents
A kind of scalable ultrasonic cell disruption instrument probe with automatic detection function Download PDFInfo
- Publication number
- CN109294886A CN109294886A CN201811078221.5A CN201811078221A CN109294886A CN 109294886 A CN109294886 A CN 109294886A CN 201811078221 A CN201811078221 A CN 201811078221A CN 109294886 A CN109294886 A CN 109294886A
- Authority
- CN
- China
- Prior art keywords
- ultrasonic
- probe
- cell disruption
- disruption instrument
- ultrasonic cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
Abstract
The invention discloses a kind of probe of scalable ultrasonic cell disruption instrument with automatic detection function, it includes each model which, which is made of aluminum coating teflon material,.It is not only harmless to cell during clasmatosis, but also make to pop one's head in during clasmatosis and amplitude transformer front end is not easy adherent cell, secondly cost substantially reduces.
Description
Technical field
The present invention relates to biologies, pharmaceutical experiment device field, concretely, are related to a kind of ultrasonic cell disruption instrument spy
Head.
Background technique
Ultrasonic cell disruption instrument (also referred to as ultrasonic cell disruptor, ultrasonic emulsification machine) is a kind of to lead to electric energy
It crosses energy converter and is converted into sound energy, which becomes many heavy seeds by liquid medium, these heavy seeds are fried rapidly
It splits, the energy as bomb is generated, to play the role of the substances such as disrupting tissue.Ultrasonic cell disruption instrument can be used for more
The broken of cell, bacterium and animal vegetable tissue is planted, the fields such as biology, medicine, agronomy are widely used in.
Ultrasonic cell disruption instrument is so that liquid is generated cavitation using the dispersion effect of ultrasonic wave in a liquid, from
And it is crushed solid particle or cell tissue in liquid.Ultrasonic generator is by energy converter and amplitude transformer two large divisions's group
At amplitude transformer front end is equipped with probe.Energy converter provides the alternating voltage of 15-25Hz, and by alternating voltage with the frequency of 15-25Hz
Make concertina type elastic deformation, energy converter does longitudinal mechanical oscillation simultaneously.Vibration wave by immerse biological solution in amplitude transformer and
The probe that aluminum is coated with teflon material generates cavitation effect, the biological particle high vibration in exciting media.
Existing sonicator uses titanium alloy amplitude transformer and probe, its shortcoming is that, first in clasmatosis process
In be easy adherent cell；Secondly manufacturing cost is higher.
Summary of the invention
The present invention is popped one's head in using aluminum coating teflon material, not only harmless to cell, but also makes clasmatosis process
Middle probe and amplitude transformer front end are not easy adherent cell, and secondly cost substantially reduces.
The purpose of the present invention is achieved through the following technical solutions:
A kind of scalable band automatic detection function Probe Ultrasonic Searching wave cell crushing instrument of band, including cabinet, affiliated cabinet include that can rise
The electric push rod of drop, ultrasonic generator are fixedly connected by electric push rod with box house.It is characterized in that, described
There are a pallet in setting connection ultrasonic generator below electric push rod, lower section, and pallet is for placing beaker container.Ultrasonic wave
Generating device includes ultrasonic transducer, amplitude transformer, ultrasonic probe.Its ultrasonic probe is coated with teflon material using aluminium wire
It is made.Ultrasonic transducer is arranged on electric push rod, and amplitude transformer is arranged in ultrasonic transducer lower end, and ultrasonic probe is set
It sets in amplitude transformer lower end.Ultrasonic cell disruption instrument probe is divided into telescopic cylindrical probe and telescopic cone is visited
Head, ultrasonic cell disruption instrument probe band automatic detection function, the automatic detection container of the first step and is contained when starting to work every time
The volume of content, probe extends under liquid level or organization internal stops, and prevents probe from deeply causing the destruction of container.Probe
Length and diameter be all it is adjustable, length is from 5cm -25cm, and diameter is from 1mm -30mm.
Preferably, ultrasonic cell disruption instrument probe is divided into telescopic cylindrical probe and telescopic cone is visited
Head.
Preferably, ultrasonic cell disruption instrument probe band automatic detection function, the first step is examined automatically when starting to work every time
The volume of xylometer and contained content, probe extends under liquid level or organization internal stops, and prevents probe from deeply causing to hold
The destruction of device.The length and diameter of probe be all it is adjustable, length is from 5cm -25cm, and diameter is from 1mm -30mm.
Preferably, thermoregulator and temperature sensor are housed in box house surrounding, thermoregulator is divided into refrigeration
Device or heat-producing machine, for carrying out monitoring temperature to box house and adjusting.
Preferably, it is provided with semiconductor cooler in box house, ultrasonic wave can be made by semiconductor cooler
The inside of case carries out fast cooling, and avoiding the problem that internal temperature is excessively high leads to element function degeneration and aging circuit, increases
It the working time of ultrasonic cell disruption instrument, improves work efficiency and the service life of sonicator.
Preferably, box house is also equipped with ultraviolet lamp, and ultraviolet lamp can carry out sterilizing processing to box house, avoid
Case Endophytic bacteria is influenced caused by Protein Extraction, reduces the quality and production capacity of protein.
Preferably, box house is also equipped with container clamping device, can be to the appearance of different-diameter by container clamping device
Device is fixedly clamped, and avoids effectively increasing the peace of cell extraction because vibration leads to splashing down for liquid in container in clasmatosis
Quan Xing.
Preferably, it is equipped with the all-in-one machine of display panel and control panel outside the enclosure.
Detailed description of the invention
Attached drawing is used to provide further understanding of the present invention, and constitutes a part of the invention, the embodiment with invention
It is used to explain the present invention, is not construed as limiting the invention together.
Fig. 1 is ultrasonic cell disruption instrument overall structure diagram of the invention.A is ultrasonic cell disruption instrument in Fig. 1
Switch；B is the all-in-one machine of control panel and display panel；C is tool housing；D is the part of transducing head connection amplitude transformer；e,f
It is ultraviolet lamp tube respectively；H is container clamping device；I is the outer radiator fan of cabinet of semiconductor cooler；G is supersonic cell
Broken instrument probe；K is the door of cabinet, the positive glass of door be it is transparent, facilitate observation clasmatosis situation；L is beaker container,
For holding tissue etc.；M is cabinet base.
Fig. 2 is ultrasonic probe schematic diagram of the invention.Wherein figure a is cylindrical scalable probe；Figure b is cone
Scalable probe.
Specific embodiment
With reference to the accompanying drawing, a specific embodiment of the invention is described in further detail.Following implementation is used for
Illustrate the present invention, but is not intended to limit the scope of the invention.
As shown in Figure 1, being ultrasonic cell disruption instrument overall structure diagram of the invention.Fig. 2 is that two kinds of exemplary ultrasonic waves are thin
Born of the same parents are crushed instrument probe schematic diagram.
As shown in Figure 1, ultrasonic cell disruption instrument probe of the invention is installed on the amplitude transformer front end of the instrument.Ultrasonic wave
Broken instrument includes that display panel and operation panel all-in-one machine, affiliated cabinet include liftable outside for several components and cabinet in cabinet
Electric push rod, ultrasonic generator is fixedly connected by electric push rod with box house.It is characterized in that, the electricity
There are a pallet in setting connection ultrasonic generator below dynamic push-pull rod, lower section, and pallet is for placing the containers such as beaker.Ultrasonic wave
Generating device includes ultrasonic transducer, amplitude transformer, ultrasonic probe.Ultrasonic transducer is arranged on electric push rod, becomes
The setting of width bar is arranged in ultrasonic transducer lower end, ultrasonic probe in amplitude transformer lower end.Its ultrasonic probe uses aluminium wire packet
It is made by teflon material.Ultrasonic cell disruption instrument probe band automatic detection function, the first step is automatic when starting to work every time
The volume of detection container and contained content, probe extends under liquid level or organization internal stops, and prevents probe from deeply causing
The destruction of container.The length and diameter of probe be all it is adjustable, for length from 5cm -25cm, diameter is equal from 1mm -30mm
It can.
Thermoregulator and temperature sensor are housed in box house surrounding, thermoregulator is divided into refrigerator or heating
Device, for carrying out monitoring temperature to box house and adjusting.It is provided with semiconductor cooler in box house, by semiconductor system
Cooler can make ultrasonic wave occur case inside carry out fast cooling, avoid internal temperature it is excessively high cause element function degenerate and
The problem of aging circuit, increases the working time of ultrasonic cell disruption instrument, improves work efficiency and ultrasonic disruption
The service life of instrument.Box house is also equipped with ultraviolet lamp, and ultraviolet lamp can carry out degerming antivirus processing to box house, avoid case
Endophytic bacteria is influenced caused by Protein Extraction, reduces the quality and production capacity of protein.Box house is also equipped with container clamping
Device can be fixedly clamped to the container of different-diameter by container clamping device, avoid in clasmatosis because vibration causes
Liquid splashes down in container, effectively increases the safety of cell extraction.It is equipped with display panel and control panel outside the enclosure.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, without departing from the technical principles of the invention, it can make several improvements and replace, these are improved and replacement is also answered
It is considered as protection scope of the present invention.
Claims (11)
1. a kind of scalable band of band detects the ultrasonic cell disruption instrument of the probe of position, including cabinet, affiliated cabinet packet automatically
Liftable electric push rod is included, ultrasonic generator is fixedly connected by electric push rod with cabinet inside；Electric push-and-pull
There are a pallet in setting connection ultrasonic generator below bar, lower section, and pallet is for placing beaker container；Ultrasonic cell-break
The ultrasonic generator of instrument includes ultrasonic transducer, amplitude transformer, ultrasonic probe；Ultrasonic probe is coated with special using aluminium wire
Fluorine dragon material is made.
2. ultrasonic cell disruption instrument according to claim 1, which is characterized in that ultrasonic generator includes ultrasonic wave
Energy converter, amplitude transformer, ultrasonic probe.
3. ultrasonic cell disruption instrument according to claim 1, which is characterized in that ultrasonic transducer setting is pushed away electronic
On pull rod, amplitude transformer setting is arranged in ultrasonic transducer lower end, ultrasonic probe in amplitude transformer lower end.
4. ultrasonic cell disruption instrument according to claim 1, which is characterized in that ultrasonic probe is coated with special using aluminium wire
Fluorine dragon material is made.
5. ultrasonic cell disruption instrument probe according to claim 1, which is characterized in that ultrasonic cell disruption instrument probe
It is divided into telescopic cylindrical probe and telescopic cone probe, the automatic detection function of ultrasonic cell disruption instrument probe band
Can, the automatic detection container of the first step and the volume of contained content when starting to work every time, probe extend in liquid level or tissue
Portion stops, and prevents probe from deeply causing the destruction of container.
6. ultrasonic cell disruption instrument probe according to claim 1, which is characterized in that the length of sonicator probe
Degree and diameter be all it is adjustable, length is from 5cm -25cm, and diameter is from 1mm -30mm.
7. a kind of ultrasonic cell disruption instrument according to claim 1, which is characterized in that in cabinet inside surrounding equipped with temperature
Degree adjuster and temperature sensor, thermoregulator are divided into refrigerator or heat-producing machine, for carrying out temperature prison to box house
Control and adjusting.
8. a kind of ultrasonic cell disruption instrument according to claim 1, which is characterized in that be provided in box house and partly lead
Chiller.
9. a kind of ultrasonic cell disruption instrument according to claim 1, which is characterized in that box house is also equipped with ultraviolet
Lamp.
10. a kind of ultrasonic cell disruption instrument according to claim 1, which is characterized in that box house is also equipped with container
Clamping device, the containers such as beaker for different-diameter are fixed, and prevent container during clasmatosis from shaking caused liquid
Drop is splashed, and the safety of cell extraction is effectively increased.
11. a kind of ultrasonic cell disruption instrument according to claim 1, which is characterized in that be equipped with display surface outside the enclosure
The all-in-one machine of plate and control panel.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811078221.5A CN109294886A (en) | 2018-09-17 | 2018-09-17 | A kind of scalable ultrasonic cell disruption instrument probe with automatic detection function |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811078221.5A CN109294886A (en) | 2018-09-17 | 2018-09-17 | A kind of scalable ultrasonic cell disruption instrument probe with automatic detection function |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109294886A true CN109294886A (en) | 2019-02-01 |
Family
ID=65163215
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811078221.5A Pending CN109294886A (en) | 2018-09-17 | 2018-09-17 | A kind of scalable ultrasonic cell disruption instrument probe with automatic detection function |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109294886A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006334268A (en) * | 2005-06-06 | 2006-12-14 | Shinshu Univ | Ultrasonic-driven scalpel actively curving and extending superelastic alloy vibration transmitter by using linking mechanism |
| CN201644188U (en) * | 2010-03-30 | 2010-11-24 | 陈元平 | Ultrasonic crusher with multiple transmitters |
| CN207287685U (en) * | 2017-08-29 | 2018-05-01 | 华夏源（上海）干细胞技术有限公司 | A kind of sub-cooled circulating ultrasonic crusher |
| CN107999249A (en) * | 2017-12-29 | 2018-05-08 | 武汉纤然纤维素有限公司 | A kind of Ultrasonic Intelligent crushes instrument |
| CN207619416U (en) * | 2017-12-12 | 2018-07-17 | 湛江中科技术服务有限公司 | A kind of ultrasonic cell disruption instrument |
-
2018
- 2018-09-17 CN CN201811078221.5A patent/CN109294886A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006334268A (en) * | 2005-06-06 | 2006-12-14 | Shinshu Univ | Ultrasonic-driven scalpel actively curving and extending superelastic alloy vibration transmitter by using linking mechanism |
| CN201644188U (en) * | 2010-03-30 | 2010-11-24 | 陈元平 | Ultrasonic crusher with multiple transmitters |
| CN207287685U (en) * | 2017-08-29 | 2018-05-01 | 华夏源（上海）干细胞技术有限公司 | A kind of sub-cooled circulating ultrasonic crusher |
| CN207619416U (en) * | 2017-12-12 | 2018-07-17 | 湛江中科技术服务有限公司 | A kind of ultrasonic cell disruption instrument |
| CN107999249A (en) * | 2017-12-29 | 2018-05-08 | 武汉纤然纤维素有限公司 | A kind of Ultrasonic Intelligent crushes instrument |
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Application publication date: 20190201 |