CN109289952B - Preparation method of microfluidic chip with high-viscosity micron-sized channel - Google Patents
Preparation method of microfluidic chip with high-viscosity micron-sized channel Download PDFInfo
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- CN109289952B CN109289952B CN201811322667.8A CN201811322667A CN109289952B CN 109289952 B CN109289952 B CN 109289952B CN 201811322667 A CN201811322667 A CN 201811322667A CN 109289952 B CN109289952 B CN 109289952B
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- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- 239000002131 composite material Substances 0.000 claims abstract description 11
- 239000004205 dimethyl polysiloxane Substances 0.000 claims abstract description 9
- 235000013870 dimethyl polysiloxane Nutrition 0.000 claims abstract description 9
- CXQXSVUQTKDNFP-UHFFFAOYSA-N octamethyltrisiloxane Chemical compound C[Si](C)(C)O[Si](C)(C)O[Si](C)(C)C CXQXSVUQTKDNFP-UHFFFAOYSA-N 0.000 claims abstract description 9
- 238000004987 plasma desorption mass spectroscopy Methods 0.000 claims abstract description 9
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 claims abstract description 9
- 230000015572 biosynthetic process Effects 0.000 claims abstract description 7
- DCAYPVUWAIABOU-UHFFFAOYSA-N hexadecane Chemical compound CCCCCCCCCCCCCCCC DCAYPVUWAIABOU-UHFFFAOYSA-N 0.000 claims description 6
- FHVDTGUDJYJELY-UHFFFAOYSA-N 6-{[2-carboxy-4,5-dihydroxy-6-(phosphanyloxy)oxan-3-yl]oxy}-4,5-dihydroxy-3-phosphanyloxane-2-carboxylic acid Chemical compound O1C(C(O)=O)C(P)C(O)C(O)C1OC1C(C(O)=O)OC(OP)C(O)C1O FHVDTGUDJYJELY-UHFFFAOYSA-N 0.000 claims description 3
- NWGKJDSIEKMTRX-AAZCQSIUSA-N Sorbitan monooleate Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O NWGKJDSIEKMTRX-AAZCQSIUSA-N 0.000 claims description 3
- 229940072056 alginate Drugs 0.000 claims description 3
- 235000010443 alginic acid Nutrition 0.000 claims description 3
- 229920000615 alginic acid Polymers 0.000 claims description 3
- 238000010008 shearing Methods 0.000 claims description 3
- 239000007788 liquid Substances 0.000 abstract description 15
- 238000000034 method Methods 0.000 abstract description 7
- 238000006243 chemical reaction Methods 0.000 abstract description 4
- 238000003786 synthesis reaction Methods 0.000 abstract description 4
- 239000010410 layer Substances 0.000 description 26
- 238000004519 manufacturing process Methods 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 2
- 239000002105 nanoparticle Substances 0.000 description 2
- 239000012620 biological material Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/0241—Drop counters; Drop formers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
- B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
- B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Dispersion Chemistry (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Physical Or Chemical Processes And Apparatus (AREA)
Abstract
A preparation method of a microfluidic chip of a high-viscosity micron-sized channel relates to a preparation method of a micron-sized experimental apparatus, wherein the chip is made of PDMS, and the chip structure is divided into an upper layer and a lower layer; the micro-fluidic chip of the micro-channel is composed of an upper layer and a lower layer of PDMS chip, wherein the upper layer of the chip is provided with a cross structure which is in a micro-level and generates micro-liquid drops, the lower layer of the chip is provided with a long channel with a triangular top end, the channel is the same width and the same depth as the upper layer of the channel, then the lower layer of the channel is aligned to the A part of the upper layer of the chip under a microscope, and the structure of the micro-liquid drops is formed, and the micro-fluidic chip is sealed to form the composite double-layer chip. The micro-fluidic chip of the micro-scale channel which can be manufactured by the conventional laboratory can be used for preparing the high-viscosity micro-droplet, the method is simple and easy to implement, and the method can be widely applied to the fields of biology, pharmaceutical chemistry, chemical reaction, synthesis and the like in future.
Description
Technical Field
The invention relates to a preparation method of a micron-sized experimental apparatus, in particular to a preparation method of a microfluidic chip of a high-viscosity micron-sized channel.
Background
Microfluidic chip systems are capable of producing highly dispersed microdroplets efficiently, rapidly and controllably over a nano-or micro-diameter range. The specific surface area of the liquid drop is large, the heat and mass transfer distance is very short, the reaction is quick, and the liquid drop has certain advantages in chemical and biological application. In recent years, microfluidic micro-droplet technology is widely applied in fields of biology, pharmaceutical chemistry, chemical reaction, synthesis and the like, and along with the development of science, the demand for high-viscosity micro-droplets is increasing, such as high polymer solutions, biological materials for synthesis and the like, but due to the relatively large viscosity of the liquid, nano-sized channels are usually required for obtaining micro-sized droplets, and a series of special and expensive instruments and equipment are required for the nano-sized microfluidic chip, so that the conventional single-layer chip cannot generate stable and uniform micro-droplets, and the experiment needs cannot be met, so that the experiment is difficult to realize in a general conventional laboratory.
Disclosure of Invention
The invention aims to provide a preparation method of a microfluidic chip with a high-viscosity micron-sized channel, and the composite double-layer chip prepared by the preparation method can generate uniform micro-droplets with the volume of only 1.1pL, and can stably obtain the micro-droplets with the high viscosity and the very small volume.
The invention aims at realizing the following technical scheme:
a method for preparing a microfluidic chip with a high-viscosity micron-sized channel, which comprises the following preparation processes:
the micron-sized micro-droplet manufacturing upper chip is in a cross channel shape, and is in a straight channel shape with the lower chip which is the same width and the same depth and is pointed at the top end, and the micro-sized micro-droplet manufacturing upper chip and the lower chip are sealed together at the position where the micro-droplet is formed to form a composite double-layer chip; namely: the chip is made of PDMS, and the chip structure is divided into an upper layer and a lower layer; the micro-fluidic chip of the micro-channel is composed of an upper layer and a lower layer of PDMS chip, wherein the upper layer of the chip is provided with a cross structure which is in a micro-level and generates micro-liquid drops, the lower layer of the chip is provided with a long channel with a triangular top end, the channel is the same width and the same depth as the upper layer of the channel, then the lower layer of the channel is aligned to the A part of the upper layer of the chip under a microscope, and the structure of the micro-liquid drops is formed, and the micro-fluidic chip is sealed to form the composite double-layer chip.
According to the preparation method of the microfluidic chip with the high-viscosity micron-sized channel, the upper chip is of a micron-sized cross structure for generating micro-droplets, the lower chip is of a long channel with a triangular top end, the long channel is the same as the upper channel in width and depth, and then the lower channel is aligned with the A position of the upper chip under a microscope for sealing.
The A part is a structure part for forming micro liquid drops; at the micro-droplet formation, the channel suddenly deepens and the shear force increases, causing the droplet to break.
According to the preparation method of the microfluidic chip of the high-viscosity micron-sized channel, hexadecane containing 3% (w/v) SPAN80 is used as a continuous phase, 2% alginate is used as a disperse phase to form micro-droplets, the flow rate condition is that the flow rate of the continuous phase is 5 mu L/min, and the flow rate of the disperse phase is 0.1 mu L/min, and uniform micro-droplets with the volume of 1.1pL are generated.
The invention has the advantages and effects that:
1. the composite double-layer chip prepared by the invention has the advantages that at the micro-droplet forming position, the channel suddenly deepens, the shearing force becomes large, the droplet is promoted to be broken and formed, the micro-droplet with very small volume and high viscosity can be stably obtained, the manufacturing method is very simple, the manufacturing can be performed in a conventional laboratory, and expensive and complex instruments and equipment are not needed.
2. The micro-fluidic chip of the micro-scale channel which can be manufactured by the conventional laboratory can be used for preparing the high-viscosity micro-droplet, the method is simple and easy to implement, and the method can be widely applied to the fields of biology, pharmaceutical chemistry, chemical reaction, synthesis and the like in future.
Drawings
FIG. 1 is a front view of an upper chip of a microfluidic chip according to the present invention;
FIG. 2 is a side view of an upper chip of the microfluidic chip of the present invention;
FIG. 3 is a perspective view of a top chip of the microfluidic chip of the present invention;
FIG. 4 is a front view of a lower chip of the microfluidic chip of the present invention;
FIG. 5 is a side view of a lower chip of the microfluidic chip of the present invention;
FIG. 6 is a perspective view of a lower chip of the microfluidic chip of the present invention;
FIG. 7 is a front view of a composite double-layer microfluidic chip of the present invention;
FIG. 8 is a side view of a composite double-layer microfluidic chip of the present invention;
fig. 9 is a perspective view of a composite double-layer microfluidic chip according to the present invention.
Detailed Description
The present invention will be described in detail with reference to examples.
The invention is a micro-fluidic chip method of micron-scale channel that can make the high-viscosity micro-droplet steadily, this chip is made of PDMS, or utilize this method can also choose other materials to prepare the chip, the chip structure is divided into upper and lower two-layer; the micro-fluidic chip of the micro-channel is composed of an upper layer and a lower layer of PDMS chip, wherein the upper layer of the chip is provided with a cross structure for generating micro-liquid drops, the lower layer of the chip is provided with a long channel with a triangular top end, the channel is the same width and the same depth as the upper layer of the channel, then the lower layer of the channel is aligned to the A part of the upper layer of the chip under a microscope to form a micro-liquid drop structure, and the micro-liquid drop structure is sealed, so that the channel suddenly deepens at the micro-liquid drop forming part, the shearing force is increased, and the liquid drops are caused to be broken and formed.
The invention seals the cross channel (upper chip) for manufacturing micro-liquid drop with the straight channel (lower chip) with same width and same depth and pointed top at the position of forming micro-liquid drop to form the composite double-layer chip.
The medium conditions are as follows: the composite double-layer chip has 3% (w/v) SPAN80 hexadecane as a continuous phase, 2% alginate as a disperse phase to form micro-droplets, and the flow rate condition is that the flow rate of the continuous phase is 5 mu L/min and the flow rate of the disperse phase is 0.1 mu L/min. Uniform micro-droplets with the volume of only 1.1pL are generated, micro-droplets with very small volume and high viscosity can be stably obtained, and the chip can be manufactured simply and conveniently in a laboratory without expensive and complex instruments.
Claims (1)
1. The preparation method of the microfluidic chip of the high-viscosity micron-sized channel is characterized by comprising the following preparation processes:
the chip is made of PDMS, and the chip structure is divided into an upper layer and a lower layer; the microfluidic chip of the micron-sized channel is composed of an upper layer PDMS chip and a lower layer PDMS chip, wherein the upper layer chip is provided with a micron-sized cross channel for generating micro-droplets, the lower layer chip is provided with a long channel with a triangular top end, the channel is as wide as the upper layer channel, the channel is as deep as the upper layer channel, then the lower layer channel is aligned to the A part of the upper layer chip under a microscope, the A part is the cross point of the cross channel and the structure part for forming the micro-droplets, the structure part of the micro-droplets is sealed into a composite double-layer chip, the channel suddenly deepens at the formation part of the micro-droplets, and the shearing force is increased to promote the breaking formation of the droplets;
the microdroplet contains 3% (w/v) SPAN80 in hexadecane as the continuous phase and 2% alginate as the dispersed phase to form microdroplet, wherein the flow rate condition is that the flow rate of the continuous phase is 5 μL/min and the flow rate of the dispersed phase is 0.1 μL/min, and uniform microdroplet with volume of 1.1pL is produced.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811322667.8A CN109289952B (en) | 2018-11-08 | 2018-11-08 | Preparation method of microfluidic chip with high-viscosity micron-sized channel |
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|---|---|---|---|
| CN201811322667.8A CN109289952B (en) | 2018-11-08 | 2018-11-08 | Preparation method of microfluidic chip with high-viscosity micron-sized channel |
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| Publication Number | Publication Date |
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| CN109289952A CN109289952A (en) | 2019-02-01 |
| CN109289952B true CN109289952B (en) | 2024-03-08 |
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| CN103611586A (en) * | 2013-12-06 | 2014-03-05 | 山东省科学院海洋仪器仪表研究所 | Preparation method of high-smoothness and low-deformation polymer micro-fluidic optical chip |
| CN106000485A (en) * | 2016-05-12 | 2016-10-12 | 武汉大学 | Hybrid microfluidic chip and method for preparing dish-shaped double-color microspheres by means of hybrid microfluidic chip |
| CN107126987A (en) * | 2017-05-19 | 2017-09-05 | 清华大学深圳研究生院 | Three-dimensional focal stream synthesis drop micro-fluidic chip and preparation method thereof |
| CN107206334A (en) * | 2014-12-02 | 2017-09-26 | 哈恩-希卡尔特应用研究学会公司 | Apparatus and method for producing drop |
| CN108160124A (en) * | 2016-12-07 | 2018-06-15 | 中国科学院大连化学物理研究所 | Micro-fluidic chip with gradual change microchannel height, its preparation template and method |
-
2018
- 2018-11-08 CN CN201811322667.8A patent/CN109289952B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1865924A (en) * | 2006-06-13 | 2006-11-22 | 清华大学 | Method for making micro-fluidic chip with Z-shape spectrophotometric detection cell |
| CN101609088A (en) * | 2008-06-16 | 2009-12-23 | 索尼株式会社 | Flow sending method in micro-fluidic chip and the micro-fluidic chip |
| CN103611586A (en) * | 2013-12-06 | 2014-03-05 | 山东省科学院海洋仪器仪表研究所 | Preparation method of high-smoothness and low-deformation polymer micro-fluidic optical chip |
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| CN106000485A (en) * | 2016-05-12 | 2016-10-12 | 武汉大学 | Hybrid microfluidic chip and method for preparing dish-shaped double-color microspheres by means of hybrid microfluidic chip |
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| CN109289952A (en) | 2019-02-01 |
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