CN109283145B - Ranunculus asiaticus flower color test period determination method and system - Google Patents
Ranunculus asiaticus flower color test period determination method and system Download PDFInfo
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Abstract
The invention discloses a method and a system for determining a testing period of a flower color of ranunculus asiaticus. The method comprises the following steps: determining a test area of the ranunculus asiaticus to be tested; measuring the brightness, the red-green degree and the yellow-blue degree of the test area by a color difference meter; calculating chroma and color value according to the brightness, the red-green degree and the yellow-blue degree; calculating the dynamic value of the change speed of the color value of each flowering stage of the ranunculus asiaticus to be detected according to the dynamic model of the change speed; determining a first time period; the first time period represents three continuous stages with minimum sum of absolute values of the dynamic values of the change speed; selecting the stage with the minimum absolute value of the dynamic value of the change speed from the first period as a second period; and the second period is the flower color test period of the ranunculus asiaticus to be tested. The invention overcomes the problem of lack of a DUS test sampling period of the flower color of the ranunculus asiaticus at present and provides a scientific and accurate method for testing the flower color and sampling period.
Description
Technical Field
The invention relates to the field of determination of a flower color test sampling period, in particular to a method and a system for determining a flower color test period of Ranunculus asiaticus.
Background
Ranunculus asiaticus belongs to the genus Ranunculus of the family Ranunculaceae, and is a flower used in potted plants, cut flowers and flower beds which have been developed rapidly in recent years. The ranunculus asiaticus flower color character expression range is wide, and the ranunculus asiaticus flower color has rare color lines such as green, brown, violet and the like besides common color lines; the same color system has light, medium and dark transition colors. Like other ornamental plants, buttercup flowers show a constant change in color throughout the flowering process. However, the Ranunculus DUS test guidelines do not specifically indicate a specific time period for the floral test. The flower color data obtained at different periods in the flowering process are obviously different, so that the problem of non-uniform flower color test data is caused. In the traditional practical operation, the method for fixing the flower color testing period as the pollen scattering period is also not scientific, on one hand, because the stigmas, anthers and other organs of some Ranunculus asiaticus varieties are degenerated, and some varieties are overweight petal varieties, the stigmas and anthers are difficult to observe in the whole flowering period. On the other hand, the pollen scattering period is not necessarily a period when the flower color is sufficiently expressed, and the obtained flower color is not necessarily representative. Therefore, the selection of which period in the flowering process is used as the period for testing the flower color of Ranunculus asiaticus becomes a troublesome problem in DUS testing of flower color of Ranunculus asiaticus. The key point of obtaining scientific and accurate flower color data and variety description is to establish effective variety information database to strike the break of the suit infringement.
In recent years, the research on the color change of carnation, peony and pansy in the flowering process has been greatly developed, and the research on the color change of buttercup has not been reported. To date, there has not been a method for determining the DUS test sampling period of Ranunculus flower color or a similar method.
Disclosure of Invention
The invention aims to provide a method and a system for determining a buttercup flower color test period, which overcome the problem that the current sampling period of a buttercup flower color DUS test is short and provide a scientific and accurate method for testing and sampling the flower color.
In order to achieve the purpose, the invention provides the following scheme:
a method for determining the timing of a test for a flower color of ranunculus asiaticus, said method comprising:
determining a test area of the ranunculus asiaticus to be tested;
measuring the brightness, the red-green degree and the yellow-blue degree of the test area by a color difference meter;
calculating chroma and color value according to the brightness, the red-green degree and the yellow-blue degree;
calculating the dynamic value of the change speed of the color value of each flowering stage of the ranunculus asiaticus to be detected according to the dynamic model of the change speed;
determining a first time period; the first time period represents three continuous stages with minimum sum of absolute values of the dynamic values of the change speed;
selecting the stage with the minimum absolute value of the dynamic value of the change speed from the first period as a second period; and the second period is the flower color test period of the ranunculus asiaticus to be tested.
Optionally, the test area is the central position of one third of the upper surface of the outer petals of the ranunculus asiaticus to be tested.
Optionally, the determining the brightness, the red-green degree, and the yellow-blue degree of the test area by the color difference meter specifically includes:
determining an initial measurement period of the colorimeter; determining the time for expanding the Japanese cypress of the buttercup to be detected as the initial detection period;
determining the measuring time of the color difference meter;
and measuring the brightness, the red-green degree and the yellow-blue degree of the test area at the measuring time at preset time intervals from the initial measuring period.
Optionally, the change speed dynamic model is:
Yjv＝sv(tj,tj+1)·λ(aj)；
wherein,
wherein, YjvRepresenting the dynamic value of the speed of change, sv(tj,tj+1) Represents the change speed state value, lambda (a), from the jth to the j +1 th flowering stagej) Represents the variation speed trend value of the jth flowering stage, tjDenotes the jth flowering stage, vjRepresenting the speed of the jth flowering stage, ajRepresents the acceleration of the jth flowering stage, ljRepresents the jth flowering stage measurement, 2.
A ranunculus flowerinus flower color test period determining system, comprising:
the test area determination module is used for determining a test area of the ranunculus asiaticus to be tested;
the measuring module is used for measuring the brightness, the red-green degree and the yellow-blue degree of the test area through a color difference meter;
the first calculation module is used for calculating the chroma and the color value according to the brightness, the red-green degree and the yellow-blue degree;
the second calculation module is used for calculating the change speed dynamic value of the color value of the ranunculus asiaticus to be detected in each flower forming stage according to the change speed dynamic model;
a first period determination module for determining a first period; the first time period represents three continuous stages with minimum sum of absolute values of the dynamic values of the change speed;
the test period determining module is used for selecting the stage with the minimum absolute value of the dynamic value of the change speed from the first period as a second period; and the second period is the flower color test period of the ranunculus asiaticus to be tested.
Optionally, the test area is the central position of one third of the upper surface of the outer petals of the ranunculus asiaticus to be tested.
Optionally, the determining module specifically includes:
an initial measurement period determination unit configured to determine an initial measurement period of the colorimeter; determining the time for expanding the Japanese cypress of the buttercup to be detected as the initial detection period;
a measurement time determining unit for determining a measurement time of the color difference meter;
and the measuring unit is used for measuring the brightness, the red-green degree and the yellow-blue degree of the test area at the measuring time at preset time intervals from the initial measuring period.
Optionally, the change speed dynamic model is:
Yjv＝sv(tj,tj+1)·λ(aj)；
wherein,
wherein, YjvRepresenting the dynamic value of the speed of change, sv(tj,tj+1) Represents the change speed state value, lambda (a), from the jth to the j +1 th flowering stagej) Represents the variation speed trend value of the jth flowering stage, tjDenotes the jth flowering stage, vjRepresenting the speed of the jth flowering stage, ajRepresents the acceleration of the jth flowering stage, ljRepresents the jth flowering stage measurement, 2.
Compared with the prior art, the invention has the following technical effects: the invention tracks the flower color expression state in real time, calculates the flower color change speed dynamic and the color saturation according to the color difference value, and determines the period of relatively stable and saturated flower color expression as the flower color sampling period. The problem of inconsistent test data caused by the fact that a DUS test guide of ranunculus asiaticus is not subjected to detailed test is solved, and the problems that in traditional practical operation, a loose powder stage is taken as a flower color sampling stage, a specific flower color test stage cannot be fixed by anther-free varieties, the loose powder stage is not necessarily a full flower color expression stage and the like are solved.
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In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without inventive exercise.
FIG. 1 is a flowchart of a method for determining the timing of a test for a flower color of Ranunculus asiaticus according to an embodiment of the present invention;
FIG. 2 is a schematic representation of the test area of Ranunculus asiaticus according to the embodiment of the present invention;
FIG. 3 is a block diagram showing the configuration of a system for determining the flower color test timing of Ranunculus asiaticus according to an embodiment of the present invention;
FIG. 4 is a schematic diagram of a dynamic value of a change speed corresponding to a measured value of a flower color of outer petals of a "beautiful and bright Paeonia suffruticosa brick" of a Ranunculus species in an embodiment of the present invention;
FIG. 5 is a diagram showing a dynamic value of a change speed corresponding to a measured value of a flower color of outer petals of a "brilliant-ocean peony magenta" ranunculus asiaticus variety in an embodiment of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Traits are genetically expressible features or characteristics that can be clearly identified, distinguished and described in plants. Any plant has many traits, some morphological features or characteristics, and some physiochemical features or characteristics. Traits are commonly used to describe and define plant varieties.
A plant population can be called a variety only if the plant population has specificity, consistency and stability. The specificity means that more than one character of a plant variety is obviously different from the known variety; the consistency refers to that the characteristics of one plant variety are consistent in character expression among individuals in a group except for predictable natural variation; stability means that the trait remains unchanged after repeated propagation of a plant variety or at the end of a particular propagation cycle.
The DUS test of the plant variety refers to a process of evaluating the specificity, consistency and stability of the variety to be tested by using the characters in the DUS test guide according to the DUS test guide of the corresponding crop species, and describing the variety. Therefore, in order to obtain scientific and accurate variety description, DUS tests for different traits should be performed by selecting a period when each trait is sufficiently expressed and the expression state is relatively stable, so that the obtained data is representative and repeatable.
Flowering is a phenomenon in which when pollen grains in the stamens and embryo sacs in the gynoecium mature, the calyx and petals open, exposing the pistils and stamens. In the process of blooming, the petals synthesize various pigments such as flavonoid, carotenoid and alkaloid, so that various colors are formed, and the pollination of insects is attracted. The flowering process is a dynamic process of petal expansion and enlargement, pigment enrichment and petal tissue change (such as thickness, villus and the like), so that finally presented flower color also changes dynamically. Similar to other characters, the flower color expression process also goes through an enrichment phase, a stable phase and a decline phase. Flower color is an important trait for DUS testing, particularly for ornamental plants, and almost all ornamental plants rank flower color as DUS-testing trait. In order to ensure the accuracy and comparability of the test result, the time period of the flower color DUS test should be selected to be the time period of sufficient and stable flower color expression.
The invention aims to provide a method and a system for determining a buttercup flower color test period, which overcome the problem that the current sampling period of a buttercup flower color DUS test is short and provide a scientific and accurate method for testing and sampling the flower color.
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in further detail below.
As shown in fig. 1, a method for determining the timing of a buttercup flower color test comprises:
step 101: and determining the test area of the ranunculus asiaticus to be tested. The central position of the upper third of the outer petal upper surface was determined as the test area according to the DUS test guidelines requirements, as shown in FIG. 2.
Step 102: and measuring the brightness, the red-green degree and the yellow-blue degree of the test area by a color difference meter. And obtaining L (brightness), a (red-green degree) and b (yellow-blue degree) of the flower color by using a color difference meter.
Step 103: and calculating the chroma and the color value according to the brightness, the red-green degree and the yellow-blue degree. By passing
Calculating chroma and color values, wherein C*Indicating chroma, E*A color value is represented.
Step 104: and calculating the dynamic value of the change speed of the color value of each flowering stage of the ranunculus asiaticus to be detected according to the dynamic model of the change speed. The change speed dynamic model is as follows:
Yjv＝sv(tj,tj+1)·λ(aj)；
wherein,
wherein, YjvRepresenting the dynamic value of the speed of change, sv(tj,tj+1) Represents the change speed state value, lambda (a), from the jth to the j +1 th flowering stagej) Represents the variation speed trend value of the jth flowering stage, tjDenotes the jth flowering stage, vjRepresenting the speed of the jth flowering stage, ajRepresents the acceleration of the jth flowering stage, ljRepresents the jth flowering stage measurement, 2.
The model is a common model for dynamic analysis, and the method is partially modified, namely a is calculatedjWhen using absolute values, rather than the original values. The reason for the modification is mainly that the positive and negative values of the a value are irrelevant to the color quality, but the absolute value is relevant to the acceleration or deceleration of the color change.
Step 105: determining a first time period; the first period represents three consecutive stages in which the sum of absolute values of the dynamic values of the change speed is minimum.
Step 106: selecting the stage with the minimum absolute value of the dynamic value of the change speed from the first period as a second period; and the second period is the flower color test period of the ranunculus asiaticus to be tested. And determining the period with smaller absolute value of E change speed dynamic value and larger C change speed dynamic value as the DUS test period of the buttercup flower color.
The first embodiment is as follows:
selecting flower buds at the top of main stems of a buttercup heavy valve variety 'delicate beautiful Paeonia suffruticosa brick red', determining the time of expanding the pinus calyciformis as the first day, determining the central position of one third of the upper surface of the outer petals as a test area according to DUS test guidelines, measuring the color of the area by using a color difference meter during the period of 9:30am-10:00am as shown in figure 2, recording the values of L, a and b, measuring for 3 times, and taking the average value. Using the same colorimetric method, L, a, b values were measured and recorded every two days at a fixed time (9:30am-10:00am), with the time interval changing to each day from day seven. Sorting the values of L, a, b, in accordance with
Calculating chroma and color values C and E; according to one aspect Yjv＝sv(tj,tj+1)·λ(aj) Calculating to obtain dynamic value Y of change speed of EjvAs shown in table 1 and fig. 4. Wherein,
wherein, YjvRepresenting the dynamic value of the speed of change, sv(tj,tj+1) Represents the change speed state value, lambda (a), from the jth to the j +1 th flowering stagej) Indicates the jth flower formationTrend value of step change speed, tjDenotes the jth flowering stage, vjRepresenting the speed of the jth flowering stage, ajRepresents the acceleration of the jth flowering stage, ljRepresents the jth flowering stage measurement, 2.
And combining the dynamic value of the change speed and the chroma value C to determine the twelfth day as the test period of the flower color of the variety. The specific operation is as follows: and locking three periods with small absolute values of the continuous E change speed dynamic values into the eleventh, twelfth and thirteen days, selecting the twelfth day with the minimum absolute value from the three periods, wherein the twelfth day is higher in C, so that the period selection is correct, and the corresponding color difference value is the representative color of the variety. The absolute value of the change rate dynamics of L, a, b corresponding to the twelfth day was small relative to the others, as shown in fig. 4, and C was high, demonstrating that "delicate peony brick red" stably and sufficiently expresses the flower color during this period. In addition, the results of the method for measuring the color of the color card RHSCC obtained by the British Royal society of horticulture prove that the corresponding color of the color number obtained on the twelfth day has higher saturation relative to other periods, and the color number values of three consecutive days are fixed, thereby proving that the method is scientific and effective.
TABLE 1 measured values, C and E dynamic values of the flower color of outer petals of Ranunculus species "delicate and beautiful Paeonia suffruticosa brick red" during the period from bud expansion to outer petal loosening
Example two:
selecting bud at top of main stem of Ranunculus asiaticus, determining the time of expanding calyx Pini as the first day, determining the central position of one third of the upper surface of outer petal as the test area according to DUS test guideline, and using color difference meter during 9:30am-10:00am as shown in FIG. 2The color of the area was measured, and the values of L, a, b were recorded, measured 3 times, and averaged. Using the same colorimetric method, L, a, b values were measured and recorded every two days at a fixed time (9:30am-10:00am), with the time interval changing to each day from day seven. Sorting the values of L, a, b, in accordance with
Calculating chroma and color values C and E; according to one aspect Yjv＝sv(tj,tj+1)·λ(aj) Calculating to obtain dynamic value Y of change speed of EjvAs shown in table 2 and fig. 5. Wherein,
wherein, YjvRepresenting the dynamic value of the speed of change, sv(tj,tj+1) Represents the change speed state value, lambda (a), from the jth to the j +1 th flowering stagej) Represents the variation speed trend value of the jth flowering stage, tjDenotes the jth flowering stage, vjRepresenting the speed of the jth flowering stage, ajRepresents the acceleration of the jth flowering stage, ljRepresents the jth flowering stage measurement, 2.
And determining the sixth day as the flower color testing period of the variety by combining the change speed dynamic value E and the chroma value C, and specifically operating as follows: and locking three periods with small absolute values of the continuous E change speed dynamic values as eighth, ninth and tenth days, selecting the ninth day with the minimum absolute value from the three periods, wherein the ninth day is also higher in C, so that the period selection is correct, and the corresponding color difference value is the representative color of the variety. The absolute value of the change rate dynamics of L, a, b corresponding to the ninth day was small relative to the other periods, as shown in fig. 5, and C was high, demonstrating that the flower color expression of "delicate Paeonia suffruticosa magenta" was stable and sufficient in this period. In addition, the results of the method for measuring the color by using the color card RHSCC of the British Royal society of horticulture prove that the color number obtained on the ninth day has higher saturation relative to the color numbers obtained on other periods, and the color number values of three consecutive days are fixed, thereby proving that the method is scientific and effective.
TABLE 2 dynamic values of outer petal flower color, C and E change speed during the development of the "delicate and beautiful Paeonia suffruticosa magenta" bud of buttercup variety from outer petal to outer petal loosening
According to the specific embodiment provided by the invention, the invention discloses the following technical effects: the invention tracks the flower color expression state in real time, calculates the flower color change speed dynamic and the color saturation according to the color difference value, and determines the period of relatively stable and saturated flower color expression as the flower color sampling period. The problem of inconsistent test data caused by the fact that a DUS test guide of ranunculus asiaticus is not subjected to detailed test is solved, and the problems that in traditional practical operation, a loose powder stage is taken as a flower color sampling stage, a specific flower color test stage cannot be fixed by anther-free varieties, the loose powder stage is not necessarily a full flower color expression stage and the like are solved.
As shown in fig. 3, a ranunculus flower color test timing determination system includes:
a test area determining module 301, configured to determine a test area of the ranunculus asiaticus to be tested. The test area is the central position of one third of the upper surface of the outer petals of the ranunculus asiaticus to be tested.
A determination module 302, configured to determine the brightness, red-green degree, and yellow-blue degree of the test area through a color difference meter.
The determination module 302 specifically includes:
an initial measurement period determination unit configured to determine an initial measurement period of the colorimeter; determining the time for expanding the Japanese cypress of the buttercup to be detected as the initial detection period;
a measurement time determining unit for determining a measurement time of the color difference meter;
and the measuring unit is used for measuring the brightness, the red-green degree and the yellow-blue degree of the test area at the measuring time at preset time intervals from the initial measuring period.
The first calculating module 303 is configured to calculate the chroma and the color value according to the brightness, the red-green degree, and the yellow-blue degree.
The second calculating module 304 is configured to calculate a dynamic value of the change speed of the color value of each flower forming stage of the ranunculus asiaticus to be detected according to the dynamic model of the change speed. The change speed dynamic model is as follows:
Yjv＝sv(tj,tj+1)·λ(aj)；
wherein,
wherein, YjvRepresenting the dynamic value of the speed of change, sv(tj,tj+1) Represents the change speed state value, lambda (a), from the jth to the j +1 th flowering stagej) Represents the variation speed trend value of the jth flowering stage, tjDenotes the jth flowering stage, vjRepresenting the speed of the jth flowering stage, ajRepresents the acceleration of the jth flowering stage, ljRepresents the jth flowering stage measurement, 2.
A selection module 305 for determining a first time period; the first period represents three consecutive stages in which the sum of absolute values of the dynamic values of the change speed is minimum.
A test period determining module 306, configured to select, from the first period, a period with the smallest absolute value of the dynamic value of the change speed as a second period; and the second period is the flower color test period of the ranunculus asiaticus to be tested.
The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other. For the system disclosed by the embodiment, the description is relatively simple because the system corresponds to the method disclosed by the embodiment, and the relevant points can be referred to the method part for description.
The principles and embodiments of the present invention have been described herein using specific examples, which are provided only to help understand the method and the core concept of the present invention; meanwhile, for a person skilled in the art, according to the idea of the present invention, the specific embodiments and the application range may be changed. In view of the above, the present disclosure should not be construed as limiting the invention.
Claims (8)
1. A method for determining the timing of a test for a flower color of ranunculus asiaticus, comprising:
determining a test area of the ranunculus asiaticus to be tested;
measuring the brightness, the red-green degree and the yellow-blue degree of the test area by a color difference meter;
calculating chroma and color value according to the brightness, the red-green degree and the yellow-blue degree;
calculating the dynamic value of the change speed of the color value of each flowering stage of the ranunculus asiaticus to be detected according to the dynamic model of the change speed;
determining a first time period; the first time period represents three continuous stages with minimum sum of absolute values of the dynamic values of the change speed;
selecting the stage with the minimum absolute value of the dynamic value of the change speed from the first period as a second period; and the second period is the flower color test period of the ranunculus asiaticus to be tested.
2. The method for determining a test timing of a petal of Ranunculus asiaticus according to claim 1, wherein the test area is a central position of a third of an upper surface of an outer petal of Ranunculus asiaticus to be tested.
3. The method for determining the timing of a buttercup flower color test of claim 1, wherein the determining the brightness, red-green and yellow-blue of the test area by a color difference meter comprises:
determining an initial measurement period of the colorimeter; determining the time for expanding the Japanese cypress of the buttercup to be detected as the initial detection period;
determining the measuring time of the color difference meter;
and measuring the brightness, the red-green degree and the yellow-blue degree of the test area at the measuring time at preset time intervals from the initial measuring period.
4. The method for determining the timing of a buttercup flower color test of claim 1, wherein the dynamic model of rate of change is:
Yjv＝sv(tj,tj+1)·λ(aj)；
wherein,
wherein, YjvRepresenting the dynamic value of the speed of change, sv(tj,tj+1) Represents the change speed state value, lambda (a), from the jth to the j +1 th flowering stagej) Represents the variation speed trend value of the jth flowering stage, tjDenotes the jth flowering stage, vjRepresenting the speed of the jth flowering stage, ajRepresents the acceleration of the jth flowering stage, ljRepresents the jth flowering stage measurement, 2.
5. A ranunculus flowerinus flower color test period determining system, comprising:
the test area determination module is used for determining a test area of the ranunculus asiaticus to be tested;
the measuring module is used for measuring the brightness, the red-green degree and the yellow-blue degree of the test area through a color difference meter;
the first calculation module is used for calculating the chroma and the color value according to the brightness, the red-green degree and the yellow-blue degree;
the second calculation module is used for calculating the change speed dynamic value of the color value of the ranunculus asiaticus to be detected in each flower forming stage according to the change speed dynamic model;
a first period determination module for determining a first period; the first time period represents three continuous stages with minimum sum of absolute values of the dynamic values of the change speed;
the test period determining module is used for selecting the stage with the minimum absolute value of the dynamic value of the change speed from the first period as a second period; and the second period is the flower color test period of the ranunculus asiaticus to be tested.
6. The ranunculus flower color test timing determination system according to claim 5, wherein the test area is a central position of one third of the upper surface of the outer petals of the ranunculus.
7. The ranunculus asiaticus flower color test time period determination system according to claim 5, wherein said measurement module comprises:
an initial measurement period determination unit configured to determine an initial measurement period of the colorimeter; determining the time for expanding the Japanese cypress of the buttercup to be detected as the initial detection period;
a measurement time determining unit for determining a measurement time of the color difference meter;
and the measuring unit is used for measuring the brightness, the red-green degree and the yellow-blue degree of the test area at the measuring time at preset time intervals from the initial measuring period.
8. The ranunculus anthocyanin timing test period determination system of claim 5, wherein the variation speed dynamic model is:
Yjv＝sv(tj,tj+1)·λ(aj)；
wherein,
wherein, YjvRepresenting the dynamic value of the speed of change, sv(tj,tj+1) Represents the change speed state value, lambda (a), from the jth to the j +1 th flowering stagej) Represents the variation speed trend value of the jth flowering stage, tjDenotes the jth flowering stage, vjRepresenting the speed of the jth flowering stage, ajRepresents the acceleration of the jth flowering stage, ljRepresents the jth flowering stage measurement, 2.
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