CN109276759B

CN109276759B - Bionic antibacterial abdominal wall repair material

Info

Publication number: CN109276759B
Application number: CN201811426674.2A
Authority: CN
Inventors: 李晓明; 曹广秀; 牛旭锋; 樊瑜波
Original assignee: Beihang University
Current assignee: Beihang University
Priority date: 2018-11-27
Filing date: 2018-11-27
Publication date: 2020-11-24
Anticipated expiration: 2038-11-27
Also published as: CN109276759A

Abstract

The invention discloses a bionic antibacterial abdominal wall repairing material, which comprises a porcine small intestine submucosa and a chitosan-elastin fiber net layer coated on the porcine small intestine submucosa; and the chitosan-elastin fiber net layer is an electrostatic spinning nano-scale chitosan-elastin fiber net. The antibacterial ability of the obtained abdominal wall repairing material is effectively improved by combining the chitosan-elastin fiber net layer with the porcine small intestine submucosa coating; meanwhile, the mechanical strength and the degradation performance of the abdominal wall repairing material meet the abdominal wall repairing requirement, can promote angiogenesis to facilitate tissue regeneration, is beneficial to solving the existing technical bottleneck of the abdominal wall repairing material, and effectively improves the treatment effect of abdominal wall defect repair.

Description

Bionic antibacterial abdominal wall repair material

Technical Field

The invention belongs to the technical field of medical consumables, and particularly relates to a bionic antibacterial abdominal wall repair material.

Background

The development and application of abdominal wall repair materials is an important direction in current hernia and abdominal wall surgical research. The existing abdominal wall repairing patch has a plurality of problems to be solved. Their main drawback is still the low biological activity, resulting in poor vascularization and uneven cellularity of the implant itself, eventually leading to lack of integration with the surrounding tissue and tissue regeneration. The recent use of acellular matrices in abdominal wall repair represents an effort to develop biomimetic implants characterized by higher biocompatibility and improved in vivo performance. After implantation, the graft undergoes one of the following fates: (i) neovascularization and new ECM deposition; (ii) fibrosis encapsulation; (iii) resorption/degradation. Thus, an ideal graft should facilitate early neovascularization, be fully biocompatible and biomimetic to promote regenerative immune responses, rather than inflammation that may lead to fibrotic encapsulation. Furthermore, it should allow the recruitment of stem cells and adult tissue-specific cells to eventually restore tissue function. Researchers have now studied biological heuristics for biomimetic patches for abdominal wall reconstruction.

This clinical need is addressed using the general principles of bionics to create materials that better resemble the composition, structure and mechanical properties of the target tissue. Collagen and elastin are the most abundant components of the extracellular matrix (ECM) in almost all tissues in the body, providing the necessary cues for cell attachment, migration and organization. Collagen and elastin are also two major components of the abdominal wall extracellular matrix, and changes in their proportion or metabolism are associated with abdominal wall hernia. The decellularized porcine small intestine submucosa is a natural biological material rich in active factors, mainly comprises collagen, a plurality of growth factors and signal molecules, has excellent biological activity, has more outstanding biological advantages when being applied to the repair of various tissue defects, and can obtain the reconstruction of tissue structures and functions. Elastin provides elasticity and deformability to the tissue. The properties of elastin are critical to specific tissue functions (e.g. dermis, blood vessels, muscle). In addition, some evidence suggests that soluble elastin facilitates tissue regeneration by promoting angiogenesis. Therefore, the porcine small intestine submucosa and elastin composite can be used for bionic repair of abdominal wall structure and composition.

At present, a hernia repair material which can actively resist bacteria and can be applied to polluted and infected wounds is urgently needed. Abdominal wall defects under the conditions of pollution and infection are very common under the conditions of war wounds, traffic injuries, intestinal tract operations and the like, at present, most of the abdominal wall defects are firstly debrided and secondly fixed by materials, so that the treatment time is prolonged, the patient is suffered from pain for a long time, and the loss of sanitary, economic and social labor force is caused. To solve the above problems, it is necessary to introduce an antibacterial material into the abdominal wall repair material. The chitosan is a degradable material, belongs to natural polycation biological polysaccharide, and has broad-spectrum antibacterial effect. There are mainly 2 views on the antibacterial mechanism of chitosan. One is a mechanism proposed based on the polycationic nature of chitosan, targeting bacterial negatively charged cell membranes, which indicates that protonated ammonium in chitosan molecules interact with cellular negatively charged cell membranes, adsorbing bacteria, causing them to sag, deform and leak; the other is an antibacterial mechanism targeting DNA in bacterial molecules, which indicates that chitosan enters the cell after penetrating the damaged cell wall, further interacting with the cytoplasm, disrupting transcription of the bacterial DNA molecule. The chitosan also has good biocompatibility and biodegradability, can obviously improve the proliferation and migration speed of fiber cells, can promote the synthesis of extracellular matrix, and can create a good healing environment for the defect part.

Disclosure of Invention

The invention aims to provide a bionic antibacterial abdominal wall repair material which is suitable for surgical repair of hernia and abdominal wall and effectively improves the treatment effect of clinical abdominal wall defect repair.

In order to solve the technical problems, the invention is specifically realized by the following technical scheme:

a bionic antibacterial abdominal wall repairing material comprises a porcine small intestine submucosa and a chitosan-elastin fiber net layer, wherein the chitosan-elastin fiber net layer is covered on one side of the porcine small intestine submucosa in a covering mode.

The chitosan-elastin fiber net layer is a nano-scale chitosan-elastin fiber net layer, the fiber diameter of the chitosan-elastin fiber net layer is 80-230 nanometers, and the thickness of the chitosan-elastin fiber net layer is 50-300 micrometers. The nano-scale chitosan-elastin fiber net is beneficial to better exerting the antibacterial effect of the chitosan-elastin fiber layer on the porcine small intestine submucosa repairing effect.

The chitosan-elastin fiber net layer is prepared by adopting an electrostatic spinning method, and is combined with a porcine small intestine submucosa covering film, so that the medical performance of the abdominal wall repairing material is further optimized.

The porcine small intestine submucosa is 4 layers of acellular porcine small intestine submucosa materials.

The invention has the beneficial effects that:

the chitosan-elastin fiber net layer is combined with the porcine small intestine submucosa coating, so that the obtained abdominal wall repairing material has bionic property, antibacterial property and biological activity. Meanwhile, the mechanical strength and the degradation performance of the abdominal wall repairing material meet the abdominal wall repairing requirement, can promote angiogenesis to facilitate tissue regeneration, is beneficial to solving the existing technical bottleneck of the abdominal wall repairing material, and effectively improves the treatment effect of abdominal wall defect repair.

Drawings

FIG. 1 is a schematic structural diagram of a bionic antibacterial abdominal wall repair material in example 1 of the present invention; wherein 1 is porcine small intestine submucosa, and 2 is chitosan-elastin fiber net layer;

FIG. 2 is a scanning electron micrograph of submucosa of small intestine of swine according to example 1 of the present invention, wherein FIG. 2(a) is a low magnification and FIG. 2(b) is a high magnification;

FIG. 3 is a cross-sectional view of a porcine small intestine submucosa in accordance with example 1 of the present invention;

FIG. 4 is a scanning electron microscope image of the bionic antibacterial abdominal wall repair material of example 1, wherein FIG. 4(a) is at low magnification and FIG. 4(b) is at high magnification;

FIG. 5 is a cross-sectional view of the bionic antibacterial abdominal wall repair material of example 1 of the present invention.

Detailed Description

The technical solutions of the present invention will be described clearly and completely with reference to specific embodiments of the present invention, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example 1

A bionic antibacterial abdominal wall repairing material is shown in figure 1, and comprises porcine small intestine submucosa and chitosan-elastin fiber net layer coated on one side of the porcine small intestine submucosa.

The thickness of the chitosan-elastin fiber net layer is 50 microns, the fiber diameter of the nano-scale chitosan-elastin fiber is 100 nanometers, and the nano-scale chitosan-elastin fiber net is prepared by adopting electrostatic spinning. As shown in fig. 2 and 3, the porcine small intestine submucosa is 4 layers of decellularized porcine small intestine submucosa material.

As shown in fig. 4 and 5, in the scanning electron microscope images of the prepared bionic antibacterial abdominal wall repair material, it can be seen from fig. 4 that the chitosan-elastin fibers in the bionic antibacterial abdominal wall repair material have smooth surfaces and nano-scale fiber diameters; as can be seen from FIG. 5, the two layers of the bionic antibacterial abdominal wall repair material are tightly combined.

Example 2

A bionic antibacterial abdominal wall repairing material comprises porcine small intestine submucosa and chitosan-elastin fiber net layer coated on one side of the porcine small intestine submucosa

In this embodiment, the thickness of the nano-sized chitosan-elastin fiber web layer is 100 microns, the fiber diameter of the nano-sized chitosan-elastin fiber is 150 nanometers, and the nano-sized chitosan-elastin fiber web is prepared by electrostatic spinning. The porcine small intestine submucosa in this embodiment is a 4-layer decellularized porcine small intestine submucosa material.

Example 3

A bionic antibacterial abdominal wall repairing material comprises a porcine small intestine submucosa and a nano chitosan-elastin fiber net layer coated on one side of the porcine small intestine submucosa.

In this embodiment, the thickness of the nano-sized chitosan-elastin fiber web layer is 150 microns, the fiber diameter of the nano-sized chitosan-elastin fiber is 200 nanometers, and the nano-sized chitosan-elastin fiber web is prepared by electrostatic spinning. The porcine small intestine submucosa in this embodiment is a 4-layer decellularized porcine small intestine submucosa material.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The bionic antibacterial abdominal wall repair material is characterized by comprising a porcine small intestine submucosa and a chitosan-elastin fiber net layer, wherein the chitosan-elastin fiber net layer is covered on one side of the porcine small intestine submucosa.

2. The biomimetic antibacterial abdominal wall repair material as recited in claim 1, wherein the chitosan-elastin fiber mesh layer is a nano-scale chitosan-elastin fiber mesh layer.

3. The bionic antibacterial abdominal wall repair material as claimed in claim 2, wherein the chitosan-elastin fiber net layer has a fiber diameter of 80-230 nm and a thickness of 50-300 μm.

4. The bionic antibacterial abdominal wall repair material as claimed in claim 3, wherein the chitosan-elastin fiber mesh layer is prepared by electrospinning.

5. The biomimetic antibacterial abdominal wall repair material according to claim 1, wherein the porcine small intestine submucosa is a 4-layer decellularized porcine small intestine submucosa material.