CN109275911A - A kind of feeding probiotic tablet and preparation method thereof - Google Patents
A kind of feeding probiotic tablet and preparation method thereof Download PDFInfo
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- CN109275911A CN109275911A CN201811304794.5A CN201811304794A CN109275911A CN 109275911 A CN109275911 A CN 109275911A CN 201811304794 A CN201811304794 A CN 201811304794A CN 109275911 A CN109275911 A CN 109275911A
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- probiotics
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- 239000006041 probiotic Substances 0.000 title claims abstract description 128
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 128
- 230000000529 probiotic effect Effects 0.000 title claims abstract description 61
- 238000002360 preparation method Methods 0.000 title claims abstract description 20
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims abstract description 54
- 229920002472 Starch Polymers 0.000 claims abstract description 38
- HQKMJHAJHXVSDF-UHFFFAOYSA-L magnesium stearate Chemical compound [Mg+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O HQKMJHAJHXVSDF-UHFFFAOYSA-L 0.000 claims abstract description 38
- 239000008107 starch Substances 0.000 claims abstract description 38
- 235000019698 starch Nutrition 0.000 claims abstract description 38
- 239000003094 microcapsule Substances 0.000 claims abstract description 36
- 239000000546 pharmaceutical excipient Substances 0.000 claims abstract description 31
- 229920000747 poly(lactic acid) Polymers 0.000 claims abstract description 24
- 239000004626 polylactic acid Substances 0.000 claims abstract description 24
- WHNWPMSKXPGLAX-UHFFFAOYSA-N N-Vinyl-2-pyrrolidone Chemical compound C=CN1CCCC1=O WHNWPMSKXPGLAX-UHFFFAOYSA-N 0.000 claims abstract description 23
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims abstract description 22
- 229960000913 crospovidone Drugs 0.000 claims abstract description 22
- 235000013809 polyvinylpolypyrrolidone Nutrition 0.000 claims abstract description 22
- 229920000523 polyvinylpolypyrrolidone Polymers 0.000 claims abstract description 22
- 239000000661 sodium alginate Substances 0.000 claims abstract description 22
- 235000010413 sodium alginate Nutrition 0.000 claims abstract description 22
- 229940005550 sodium alginate Drugs 0.000 claims abstract description 22
- 239000011734 sodium Substances 0.000 claims abstract description 20
- 229910052708 sodium Inorganic materials 0.000 claims abstract description 20
- 229920001503 Glucan Polymers 0.000 claims abstract description 19
- 235000019359 magnesium stearate Nutrition 0.000 claims abstract description 19
- 229920000463 Poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol) Polymers 0.000 claims abstract description 18
- 229920000428 triblock copolymer Polymers 0.000 claims abstract description 17
- 239000001866 hydroxypropyl methyl cellulose Substances 0.000 claims abstract description 15
- 235000010979 hydroxypropyl methyl cellulose Nutrition 0.000 claims abstract description 15
- 229920003088 hydroxypropyl methyl cellulose Polymers 0.000 claims abstract description 15
- UFVKGYZPFZQRLF-UHFFFAOYSA-N hydroxypropyl methyl cellulose Chemical compound OC1C(O)C(OC)OC(CO)C1OC1C(O)C(O)C(OC2C(C(O)C(OC3C(C(O)C(O)C(CO)O3)O)C(CO)O2)O)C(CO)O1 UFVKGYZPFZQRLF-UHFFFAOYSA-N 0.000 claims abstract description 14
- 239000000463 material Substances 0.000 claims abstract description 10
- 239000002775 capsule Substances 0.000 claims abstract description 8
- 239000000843 powder Substances 0.000 claims description 43
- 230000001580 bacterial effect Effects 0.000 claims description 41
- 241000894006 Bacteria Species 0.000 claims description 20
- 239000006185 dispersion Substances 0.000 claims description 16
- 238000000034 method Methods 0.000 claims description 14
- 239000007788 liquid Substances 0.000 claims description 11
- 241000193468 Clostridium perfringens Species 0.000 claims description 10
- 239000012141 concentrate Substances 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 230000000243 photosynthetic effect Effects 0.000 claims description 9
- 239000002904 solvent Substances 0.000 claims description 8
- 229920001817 Agar Polymers 0.000 claims description 5
- 239000008272 agar Substances 0.000 claims description 5
- 238000000748 compression moulding Methods 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 5
- 239000001963 growth medium Substances 0.000 claims description 5
- 239000002609 medium Substances 0.000 claims description 5
- 238000004132 cross linking Methods 0.000 claims description 2
- 229960003943 hypromellose Drugs 0.000 claims 1
- 229920000036 polyvinylpyrrolidone Polymers 0.000 claims 1
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 claims 1
- 229940069328 povidone Drugs 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 7
- 210000001035 gastrointestinal tract Anatomy 0.000 abstract description 3
- 238000012360 testing method Methods 0.000 description 12
- 241000193830 Bacillus <bacterium> Species 0.000 description 10
- 235000013305 food Nutrition 0.000 description 7
- 239000012530 fluid Substances 0.000 description 6
- 239000013558 reference substance Substances 0.000 description 6
- 229920002678 cellulose Polymers 0.000 description 4
- 239000001913 cellulose Substances 0.000 description 4
- 235000013365 dairy product Nutrition 0.000 description 4
- 230000002496 gastric effect Effects 0.000 description 4
- -1 hydroxypropyl Chemical group 0.000 description 4
- 238000004090 dissolution Methods 0.000 description 3
- 235000013618 yogurt Nutrition 0.000 description 3
- 241000186000 Bifidobacterium Species 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 235000013376 functional food Nutrition 0.000 description 2
- 210000004051 gastric juice Anatomy 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 239000006916 nutrient agar Substances 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- AZUYLZMQTIKGSC-UHFFFAOYSA-N 1-[6-[4-(5-chloro-6-methyl-1H-indazol-4-yl)-5-methyl-3-(1-methylindazol-5-yl)pyrazol-1-yl]-2-azaspiro[3.3]heptan-2-yl]prop-2-en-1-one Chemical compound ClC=1C(=C2C=NNC2=CC=1C)C=1C(=NN(C=1C)C1CC2(CN(C2)C(C=C)=O)C1)C=1C=C2C=NN(C2=CC=1)C AZUYLZMQTIKGSC-UHFFFAOYSA-N 0.000 description 1
- 235000005979 Citrus limon Nutrition 0.000 description 1
- 244000131522 Citrus pyriformis Species 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 240000001046 Lactobacillus acidophilus Species 0.000 description 1
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 description 1
- 241000235070 Saccharomyces Species 0.000 description 1
- 241000235342 Saccharomycetes Species 0.000 description 1
- 235000010443 alginic acid Nutrition 0.000 description 1
- 229960001126 alginic acid Drugs 0.000 description 1
- 239000000783 alginic acid Substances 0.000 description 1
- 229920000615 alginic acid Polymers 0.000 description 1
- 150000004781 alginic acids Chemical class 0.000 description 1
- 239000007900 aqueous suspension Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000009514 concussion Effects 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 235000015140 cultured milk Nutrition 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 230000007366 host health Effects 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 150000002576 ketones Chemical class 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 229940039695 lactobacillus acidophilus Drugs 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000011056 performance test Methods 0.000 description 1
- 235000013406 prebiotics Nutrition 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 235000021404 traditional food Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/03—Organic compounds
- A23L29/035—Organic compounds containing oxygen as heteroatom
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/03—Organic compounds
- A23L29/035—Organic compounds containing oxygen as heteroatom
- A23L29/04—Fatty acids or derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/30—Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
- A23P10/00—Shaping or working of foodstuffs characterised by the products
- A23P10/30—Encapsulation of particles, e.g. foodstuff additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention discloses a kind of feeding probiotic tablet and preparation method thereof, which includes the probiotic microcapsule and pharmaceutic adjuvant that mass ratio is 1:185 ~ 215；Pharmaceutic adjuvant includes that mass ratio is 100:70 ~ 80:55 ~ 65:2 ~ 4:3 ~ 5:3 ~ 5:0.2 ~ 0.4 starch, sodium carboxymethyl starch, oligomeric glucan, citric acid, PEO-PPO-PEO triblock copolymer, hydroxypropyl methylcellulose and magnesium stearate；Probiotic microcapsule includes probiotics and the capsule material that is wrapped in outside probiotics, and capsule material includes that mass ratio is 1:2 ~ 4:9 ~ 11:4.5 ~ 5.5 polylactic acid, crospovidone and sodium alginate, can keep activity in the gastrointestinal tract.
Description
Technical field
The present invention relates to a kind of feeding probiotic tablets and preparation method thereof.
Background technique
" probiotics " is defined as microorganism living, and it is micro- that this microorganism can improve host intestine after sufficient amount by oral administration
The balance of biology, and then beneficial effect is generated to host health.Strain usually as probiotics is mostly lactic acid bacteria (such as acidophilus cream
Bacillus, Bifidobacterium etc.), some saccharomycete (such as Saccharomyces boullardii) are also classified as the scope of probiotics.
Probiotic food is to develop a field being exceedingly fast in functional food, to contain such as lactobacillus acidophilus and bifid
The exploitation of the dairy products and non-dairy food of the types probiotics such as bacillus has become a big research hotspot.If with probiotic food
Type divides the application of probiotics, then Yoghourt is undoubtedly most widely used probiotic food carrier.It is reported that in Europe
Continent, only probiotic yogurt just accounts for about the 65% of entire European functional food market.In addition to the acid as traditional food carrier
Outside milk, in recent years, probiotics is applied to more and more in non-dairy food system.
According to the universal of probiotics application effect, biology only living could generate prebiotic function.Therefore, probiotics
It must be living when being ingested, and keep activity in the gastrointestinal tract.To reach this activity for the probiotics in food
Required minimum viable count, there is various suggestions and standard.Usually, in consumption, the living cells of probiotics
Quantity needs to reach 106cfu/g.But in some cases, as long as probiotics viable count is not less than in effective period of food quality
105Cfu/g, which is recognized as, can reach probiotic effects.The official standard that several World of Food tissues propose is minimum 106~
107Cfu/g, for example, FIL/IDF (The Federation International e de Laiterie/
International Dairy Federation) standard be minimum in selling period, the food of Yoghourt one kind to contain
L.acidophilus107Cfu/g, and to contain Bifidobacterium 10 in fermented milk6cfu/g。
The one kind that is designed to provide of the application keeps active probiotic products in stomach.
Summary of the invention
The object of the present invention is to provide a kind of feeding probiotic tablets, can keep activity in the gastrointestinal tract.
Above-mentioned technical purpose of the invention has the technical scheme that
A kind of feeding probiotic tablet, the probiotic microcapsule and pharmaceutic adjuvant for being 1: 185~215 including mass ratio；
Pharmaceutic adjuvant includes starch, sodium carboxymethyl starch, oligomeric glucan, citric acid, PEO-PPO-PEO triblock copolymer
Object, hydroxypropyl methylcellulose and magnesium stearate；In pharmaceutic adjuvant, starch, sodium carboxymethyl starch, oligomeric glucan, citric acid,
The mass ratio of PEO-PPO-PEO triblock copolymer, hydroxypropyl methylcellulose and magnesium stearate be 100: 70~80: 55~65: 2~
4: 3~5: 3~5: 0.2~0.4；
Probiotic microcapsule includes probiotics bacterial powder and the capsule material that is wrapped in outside probiotics bacterial powder, capsule material include polylactic acid,
Crospovidone and sodium alginate；In probiotic microcapsule, probiotics bacterial powder, polylactic acid, crospovidone and sodium alginate
Mass ratio be 1: 2~4: 9~11: 4.5~5.5.
Further preferably are as follows: the probiotic microcapsule and pharmaceutic adjuvant for being 1: 200 including mass ratio；
In pharmaceutic adjuvant, starch, sodium carboxymethyl starch, oligomeric glucan, citric acid, PEO-PPO-PEO three block are total
The mass ratio of polymers, hydroxypropyl methylcellulose and magnesium stearate is 100: 75: 60: 3: 4: 4: 0.3；
In probiotic microcapsule, probiotics bacterial powder, polylactic acid, crospovidone and sodium alginate mass ratio be 1: 3:
10∶5.0。
Further preferably are as follows: probiotics used in the probiotics bacterial powder includes photosynthetic bacteria, bacillus and production gas
Bacillus capsulatus.
The object of the present invention is to provide a kind of preparation methods of feeding probiotic tablet.
Above-mentioned technical purpose of the invention has the technical scheme that
A kind of preparation method of feeding probiotic tablet, including following methods:
(1) probiotic microcapsule is prepared
Polylactic acid, crospovidone and sodium alginate are crushed to respectively and are crossed 100~150 meshes, polylactic acid, crosslinking is poly-
Ketone and sodium alginate mixing are tieed up, adds water dispersion uniformly to obtain aqueous dispersions, aqueous dispersions is sprayed on outside probiotics bacterial powder ,-
Drying eliminates solvent at 20~20 DEG C；In probiotic microcapsule, probiotics bacterial powder, polylactic acid, crospovidone and alginic acid
The mass ratio of sodium is 1: 2~4: 9~11: 4.5~5.5；
(2) pharmaceutic adjuvant is prepared
By starch, sodium carboxymethyl starch, oligomeric glucan, citric acid, PEO-PPO-PEO triblock copolymer, hydroxypropyl first
Cellulose and magnesium stearate crush respectively and cross 100~150 meshes, by starch, sodium carboxymethyl starch, oligomeric glucan, lemon
Acid, PEO-PPO-PEO triblock copolymer, hydroxypropyl methylcellulose and magnesium stearate are 100: 70~80: 55~65 in mass ratio:
2~4: 3~5: 3~5: 0.2~0.4 is uniformly mixed, and obtains pharmaceutic adjuvant；
(3) feeding probiotic tablet is prepared
By the pharmaceutic adjuvant in mass ratio 1: 185~215 of the probiotic microcapsule of step (1) preparation and step (2) preparation
It is uniformly mixed, compression molding.
Further preferably are as follows: the mass ratio of probiotic microcapsule and pharmaceutic adjuvant is 1: 200；
In pharmaceutic adjuvant, starch, sodium carboxymethyl starch, oligomeric glucan, citric acid, PEO-PPO-PEO three block are total
The mass ratio of polymers, hydroxypropyl methylcellulose and magnesium stearate is 100: 75: 60: 3: 4: 4: 0.3；
In probiotic microcapsule, probiotics bacterial powder, polylactic acid, crospovidone and sodium alginate mass ratio be 1: 3:
10∶5.0。
Further preferably are as follows: probiotics used in the probiotics bacterial powder includes photosynthetic bacteria, bacillus and production gas
Bacillus capsulatus.
Further preferably are as follows: the probiotics bacterial powder is prepared by the following method to obtain: by photosynthetic bacteria, bacillus and
The mixed bacteria of Bacillus perfringens is inoculated with by the 3~10% of culture volume, and ferment 20~30hr at 28~32 DEG C,
Probiotics fermention liquid is obtained, probiotics fermention liquid is obtained into concentrate in 4500~5500r/min centrifugal concentrating, by concentrate
Freeze-drying eliminates solvent at -40~-10 DEG C, obtains probiotics bacterial powder；
Culture medium uses wort agar medium.
In conclusion the invention has the following advantages:
By the way that capsule material is arranged outside probiotics bacterial powder, bacterium powder and external environment are isolated, reduce viable bacteria caused by directly contacting
The case where number decline；Using the special capsule material of the application, after being mixed with the auxiliary material in tablet, probiotics bacterial powder can be improved and exist
The survival rate of viable bacteria in simulated gastric fluid；
Micro-capsule is made in probiotics bacterial powder, tablet is made in micro-capsule and pharmaceutic adjuvant together, when dispersing in 37 DEG C of water not
Micro-capsule (i.e. without dissolution probiotics bacterial powder in 37 DEG C of water) are destroyed, and obtain the dispersion liquid for the wall that cannot not be uniformly dispersed viscously, can be reduced brewed
The loss of probiotics bacterial powder and loss of activity in the process, convenient for taking.
Specific embodiment
This specific embodiment is only explanation of the invention, is not limitation of the present invention, those skilled in the art
Member can according to need the modification that not creative contribution is made to the present embodiment after reading this specification, but as long as at this
All by the protection of Patent Law in the protection scope of invention.
Embodiment 1a: a kind of probiotics bacterial powder is prepared by the following method to obtain: by photosynthetic bacteria, bacillus and
The mixed bacteria of Bacillus perfringens is inoculated with by the 6% of culture volume, and ferment r for 24 hours at 30 DEG C, obtains probiotics hair
Probiotics fermention liquid is obtained concentrate in 5000r/min centrifugal concentrating by zymotic fluid, concentrate is lyophilized at -25 DEG C eliminate it is molten
Agent；Culture medium uses wort agar medium.
Embodiment 1b: a kind of probiotics bacterial powder is prepared by the following method to obtain: by photosynthetic bacteria, bacillus and
The mixed bacteria of Bacillus perfringens is inoculated with by the 3% of culture volume, and ferment 30hr at 28 DEG C, obtains probiotics hair
Probiotics fermention liquid is obtained concentrate in 4500r/min centrifugal concentrating by zymotic fluid, concentrate is lyophilized at -40 DEG C eliminate it is molten
Agent；Culture medium uses wort agar medium.
Embodiment 1c: a kind of probiotics bacterial powder is prepared by the following method to obtain: by photosynthetic bacteria, bacillus and
The mixed bacteria of Bacillus perfringens is inoculated with by the 10% of culture volume, and ferment 20hr at 32 DEG C, obtains probiotics
Probiotics fermention liquid is obtained concentrate in 5500r/min centrifugal concentrating, concentrate is lyophilized at -10 DEG C and is eliminated by fermentation liquid
Solvent；Culture medium uses wort agar medium.
Embodiment 2: a kind of feeding probiotic tablet is prepared by the following method to obtain:
(1) probiotic microcapsule is prepared
Polylactic acid, crospovidone and sodium alginate are crushed respectively and cross 150 meshes, by polylactic acid, crospovidone
It is mixed with sodium alginate, water dispersion is added uniformly to obtain aqueous dispersions, aqueous dispersions are sprayed on probiotics bacterial powder (from implementation
Example 1a) outside, drying eliminates solvent at -20 DEG C；In probiotic microcapsule, probiotics bacterial powder, polylactic acid, crospovidone and
The mass ratio of sodium alginate is 1: 3: 10: 5.0；
(2) pharmaceutic adjuvant is prepared
By starch, sodium carboxymethyl starch, oligomeric glucan, citric acid, PEO-PPO-PEO triblock copolymer, hydroxypropyl first
Cellulose and magnesium stearate crush respectively and cross 150 meshes, by starch, sodium carboxymethyl starch, oligomeric glucan, citric acid,
PEO-PPO-PEO triblock copolymer, hydroxypropyl methylcellulose and magnesium stearate are 100: 75: 60: 3: 4: 4: 0.3 mixed in mass ratio
It closes uniformly, obtains pharmaceutic adjuvant；
(3) feeding probiotic tablet is prepared
The pharmaceutic adjuvant in mass ratio 1: 200 of the probiotic microcapsule of step (1) preparation and step (2) preparation is mixed equal
It is even, compression molding.
Embodiment 3: a kind of feeding probiotic tablet is prepared by the following method to obtain:
(1) probiotic microcapsule is prepared
Polylactic acid, crospovidone and sodium alginate are crushed respectively and cross 120 meshes, by polylactic acid, crospovidone
It is mixed with sodium alginate, water dispersion is added uniformly to obtain aqueous dispersions, aqueous dispersions are sprayed on probiotics bacterial powder (from implementation
Example 1b) outside, drying eliminates solvent at 0 DEG C；In probiotic microcapsule, probiotics bacterial powder, polylactic acid, crospovidone and sea
The mass ratio of mosanom is 1: 2: 9: 4.5；
(2) pharmaceutic adjuvant is prepared
By starch, sodium carboxymethyl starch, oligomeric glucan, citric acid, PEO-PPO-PEO triblock copolymer, hydroxypropyl first
Cellulose and magnesium stearate crush respectively and cross 120 meshes, by starch, sodium carboxymethyl starch, oligomeric glucan, citric acid,
PEO-PPO-PEO triblock copolymer, hydroxypropyl methylcellulose and magnesium stearate are 100: 70: 55: 2: 3: 3: 0.2 mixed in mass ratio
It closes uniformly, obtains pharmaceutic adjuvant；
(3) feeding probiotic tablet is prepared
The pharmaceutic adjuvant in mass ratio 1: 185 of the probiotic microcapsule of step (1) preparation and step (2) preparation is mixed equal
It is even, compression molding.
Embodiment 4: a kind of feeding probiotic tablet is prepared by the following method to obtain:
(1) probiotic microcapsule is prepared
Polylactic acid, crospovidone and sodium alginate are crushed and sieved with 100 mesh sieve respectively, by polylactic acid, crospovidone
It is mixed with sodium alginate, water dispersion is added uniformly to obtain aqueous dispersions, aqueous dispersions are sprayed on probiotics bacterial powder (from implementation
Example 1c) outside, drying eliminates solvent at 20 DEG C；In probiotic microcapsule, probiotics bacterial powder, polylactic acid, crospovidone and
The mass ratio of sodium alginate is 1: 4: 11: 5.5；
(2) pharmaceutic adjuvant is prepared
By starch, sodium carboxymethyl starch, oligomeric glucan, citric acid, PEO-PPO-PEO triblock copolymer, hydroxypropyl first
Cellulose and magnesium stearate are crushed and are sieved with 100 mesh sieve respectively, by starch, sodium carboxymethyl starch, oligomeric glucan, citric acid,
PEO-PPO-PEO triblock copolymer, hydroxypropyl methylcellulose and magnesium stearate are 100: 80: 65: 4: 5: 5: 0.4 mixed in mass ratio
It closes uniformly, obtains pharmaceutic adjuvant；
(3) feeding probiotic tablet is prepared
The pharmaceutic adjuvant in mass ratio 1: 215 of the probiotic microcapsule of step (1) preparation and step (2) preparation is mixed equal
It is even, compression molding.
Aqueous suspension performance test
(1) test sample: embodiment 1a-1c, embodiment 2-4, reference substance 1.
Reference substance 1: a kind of probiotic microcapsule is prepared referring to the embodiment 1 of CN104856924A.
(2) test content: taking a 100ml beaker, is put into test sample (being converted into probiotics bacterial powder is 3.0g) and 37 DEG C
Water 27.0ml, shake gently sample, shake and beaker is stood into 60s after 10s, observe its phenomenon；Testing example 2-4 and right
According to bacterium powder the amount of dissolution in product 1.
(3) test result:
It adds in the beaker of embodiment 1a-1c and reunites obviously, and be stained with sticky matter in walls of beaker；
It adds and is uniformly dispersed in the beaker of embodiment 2-4, and be still able to maintain finely dispersed state standing, in beaker almost
No probiotics bacterial powder dissolution；
It adds in the beaker of reference substance 1 and reunites obviously, and be stained with sticky matter in walls of beaker, probiotics bacterial powder is molten in beaker
It is obvious out.
Artificial gastric juice resistance's test
(1) test sample: embodiment 1a-1c, embodiment 2-4, reference substance 1.
Reference substance 1: a kind of probiotic microcapsule is prepared referring to the embodiment 1 of CN104856924A.
(2) test content:
Different test samples (being converted into probiotics bacterial powder is 3.0g) are taken respectively, 37 DEG C of water 27.0ml are added, concussion is mixed
It is even；100 μ l mixing liquids are taken, are coated on nutrient agar panel, are coated with 3 plates in parallel, are cultivated at 25 DEG C after r for 24 hours with flat
Plate counting method detects viable count therein, as initial value Ao.
Different test samples (being converted into probiotics bacterial powder is 3.0g) are taken again, and 37 DEG C of simulated gastric fluid (1000ml water is added
PH=1.2 is adjusted to hydrochloric acid) 27.0ml, it is shaken on 37 DEG C of shaking tables；100 μ l are taken to mix every 30min in the sample of shaking
Liquid is coated on nutrient agar panel, is coated with 3 plates in parallel, is cultivated at 25 DEG C and is detected it with colony counting method after r for 24 hours
In viable count, as in simulated gastric fluid place after numerical value Ai.
Viable detection X:X=Ai × 100%/Ao is calculated as follows.
Test result is as shown in table 1.Table 1 shows that survival rate of the embodiment 1a-1c in 37 DEG C of simulated gastric fluids is low,
It loses activity after 2.0hr；Compared to embodiment 1a-1c, the time-to-live of embodiment 4-8 and reference substance is long, and the time that plays a role is long.
1 artificial gastric juice resistance of table test
The specific implementation of the invention is not to be limited to these illustrations for the above content, and technology belonging to the present invention is led
For the those of ordinary skill in domain, without departing from the inventive concept of the premise, a number of simple deductions or replacements can also be made,
It all shall be regarded as belonging to present invention scope of patent protection determined by the appended claims.
Claims (7)
1. a kind of feeding probiotic tablet, which is characterized in that including mass ratio be the probiotic microcapsules of 1:185 ~ 215 and medicinal
Auxiliary material；
Pharmaceutic adjuvant include starch, sodium carboxymethyl starch, oligomeric glucan, citric acid, PEO-PPO-PEO triblock copolymer,
Hydroxypropyl methylcellulose and magnesium stearate；In pharmaceutic adjuvant, starch, sodium carboxymethyl starch, oligomeric glucan, citric acid, PEO-
The mass ratio of PPO-PEO triblock copolymer, hydroxypropyl methylcellulose and magnesium stearate is 100:70 ~ 80:55 ~ 65:2 ~ 4:3 ~ 5:3
~5:0.2~0.4；
Probiotic microcapsule includes probiotics bacterial powder and the capsule material that is wrapped in outside probiotics bacterial powder, and capsule material includes polylactic acid, crosslinking
Povidone and sodium alginate；In probiotic microcapsule, probiotics bacterial powder, polylactic acid, the matter of crospovidone and sodium alginate
Amount is than being 1:2 ~ 4:9 ~ 11:4.5 ~ 5.5.
2. a kind of feeding probiotic tablet according to claim 1, which is characterized in that the benefit for being 1:200 including mass ratio
Raw bacteria microcapsule and pharmaceutic adjuvant；
In pharmaceutic adjuvant, starch, sodium carboxymethyl starch, oligomeric glucan, citric acid, PEO-PPO-PEO triblock copolymer,
The mass ratio of hydroxypropyl methylcellulose and magnesium stearate is 100:75:60:3:4:4:0.3；
In probiotic microcapsule, probiotics bacterial powder, polylactic acid, crospovidone and sodium alginate mass ratio be 1:3:10:
5.0。
3. a kind of feeding probiotic tablet according to claim 1 or 2, which is characterized in that make in the probiotics bacterial powder
Probiotics includes photosynthetic bacteria, bacillus and Bacillus perfringens.
4. a kind of preparation method of feeding probiotic tablet, which is characterized in that including following methods:
(1) probiotic microcapsule is prepared
Polylactic acid, crospovidone and sodium alginate are crushed respectively and cross 100 ~ 150 meshes, by polylactic acid, crospovidone
It is mixed with sodium alginate, adds water dispersion uniformly to obtain aqueous dispersions, aqueous dispersions are sprayed on outside probiotics bacterial powder, -20 ~ 20
Drying eliminates solvent at DEG C；In probiotic microcapsule, probiotics bacterial powder, polylactic acid, the matter of crospovidone and sodium alginate
Amount is than being 1:2 ~ 4:9 ~ 11:4.5 ~ 5.5；
(2) pharmaceutic adjuvant is prepared
By starch, sodium carboxymethyl starch, oligomeric glucan, citric acid, PEO-PPO-PEO triblock copolymer, hypromellose
Element and magnesium stearate crushes respectively and mistake 100 ~ 150 meshes, by starch, sodium carboxymethyl starch, oligomeric glucan, citric acid,
PEO-PPO-PEO triblock copolymer, hydroxypropyl methylcellulose and magnesium stearate are 100:70 ~ 80:55 ~ 65:2 ~ 4:3 in mass ratio
~ 5:3 ~ 5:0.2 ~ 0.4 is uniformly mixed, and obtains pharmaceutic adjuvant；
(3) feeding probiotic tablet is prepared
The pharmaceutic adjuvant 1:185 in mass ratio ~ 215 of the probiotic microcapsule of step (1) preparation and step (2) preparation is mixed equal
It is even, compression molding.
5. a kind of preparation method of feeding probiotic tablet according to claim 4, which is characterized in that
The mass ratio of probiotic microcapsule and pharmaceutic adjuvant is 1:200；
In pharmaceutic adjuvant, starch, sodium carboxymethyl starch, oligomeric glucan, citric acid, PEO-PPO-PEO triblock copolymer,
The mass ratio of hydroxypropyl methylcellulose and magnesium stearate is 100:75:60:3:4:4:0.3；
In probiotic microcapsule, probiotics bacterial powder, polylactic acid, crospovidone and sodium alginate mass ratio be 1:3:10:
5.0。
6. a kind of preparation method of feeding probiotic tablet according to claim 4, which is characterized in that the probiotics bacterial
Probiotics used in powder includes photosynthetic bacteria, bacillus and Bacillus perfringens.
7. a kind of preparation method of feeding probiotic tablet according to claim 4, which is characterized in that the probiotics bacterial
Powder is prepared by the following method to obtain:
The mixed bacteria of photosynthetic bacteria, bacillus and Bacillus perfringens is inoculated with by the 3 ~ 10% of culture volume,
Ferment 20 ~ 30hr at 28 ~ 32 DEG C, probiotics fermention liquid is obtained, by probiotics fermention liquid in 4500 ~ 5500r/min centrifugal concentrating
Concentrate is obtained, concentrate is lyophilized at -40 ~ -10 DEG C and eliminates solvent, obtains probiotics bacterial powder；
Culture medium uses wort agar medium.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1642437A (en) * | 2002-03-12 | 2005-07-20 | 雀巢产品有限公司 | Probiotic delivery system |
| CN101843337A (en) * | 2010-06-07 | 2010-09-29 | 陕西科技大学 | Method for preparing probiotics tablets |
| CN107647251A (en) * | 2017-08-24 | 2018-02-02 | 太和县三九药业有限公司 | A kind of preparation method of compound edible mushroom nutrient chewable tablet |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1642437A (en) * | 2002-03-12 | 2005-07-20 | 雀巢产品有限公司 | Probiotic delivery system |
| CN101843337A (en) * | 2010-06-07 | 2010-09-29 | 陕西科技大学 | Method for preparing probiotics tablets |
| CN107647251A (en) * | 2017-08-24 | 2018-02-02 | 太和县三九药业有限公司 | A kind of preparation method of compound edible mushroom nutrient chewable tablet |
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