CN109270052A - A kind of uric acid detection method based on silver nanoparticle triangular plate - Google Patents
A kind of uric acid detection method based on silver nanoparticle triangular plate Download PDFInfo
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- CN109270052A CN109270052A CN201811362081.4A CN201811362081A CN109270052A CN 109270052 A CN109270052 A CN 109270052A CN 201811362081 A CN201811362081 A CN 201811362081A CN 109270052 A CN109270052 A CN 109270052A
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- triangular plate
- silver nanoparticle
- uric acid
- detection method
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- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 title claims abstract description 78
- 229910052709 silver Inorganic materials 0.000 title claims abstract description 63
- 239000004332 silver Substances 0.000 title claims abstract description 63
- 239000002105 nanoparticle Substances 0.000 title claims abstract description 62
- LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 title claims abstract description 41
- TVWHNULVHGKJHS-UHFFFAOYSA-N Uric acid Natural products N1C(=O)NC(=O)C2NC(=O)NC21 TVWHNULVHGKJHS-UHFFFAOYSA-N 0.000 title claims abstract description 41
- 229940116269 uric acid Drugs 0.000 title claims abstract description 41
- 238000001514 detection method Methods 0.000 title claims abstract description 23
- 238000010521 absorption reaction Methods 0.000 claims abstract description 44
- ZNNZYHKDIALBAK-UHFFFAOYSA-M potassium thiocyanate Chemical compound [K+].[S-]C#N ZNNZYHKDIALBAK-UHFFFAOYSA-M 0.000 claims abstract description 25
- 238000002360 preparation method Methods 0.000 claims abstract description 4
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims description 36
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 36
- SQGYOTSLMSWVJD-UHFFFAOYSA-N silver(I) nitrate Inorganic materials [Ag+].[O-]N(=O)=O SQGYOTSLMSWVJD-UHFFFAOYSA-N 0.000 claims description 34
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 13
- 229910052708 sodium Inorganic materials 0.000 claims description 13
- 239000011734 sodium Substances 0.000 claims description 13
- 229910021642 ultra pure water Inorganic materials 0.000 claims description 13
- 239000012498 ultrapure water Substances 0.000 claims description 13
- 239000012279 sodium borohydride Substances 0.000 claims description 11
- 229910000033 sodium borohydride Inorganic materials 0.000 claims description 11
- 239000005030 aluminium foil Substances 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 6
- 238000005286 illumination Methods 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 4
- 230000008859 change Effects 0.000 claims description 2
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 claims 2
- 229910052739 hydrogen Inorganic materials 0.000 claims 2
- 239000001257 hydrogen Substances 0.000 claims 2
- 239000007788 liquid Substances 0.000 claims 2
- 150000002978 peroxides Chemical class 0.000 claims 2
- 101710134784 Agnoprotein Proteins 0.000 claims 1
- 230000035945 sensitivity Effects 0.000 abstract description 2
- 238000000034 method Methods 0.000 description 13
- 230000000694 effects Effects 0.000 description 10
- 239000008279 sol Substances 0.000 description 9
- 239000002243 precursor Substances 0.000 description 7
- ZMZDMBWJUHKJPS-UHFFFAOYSA-N thiocyanic acid Chemical compound SC#N ZMZDMBWJUHKJPS-UHFFFAOYSA-N 0.000 description 6
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- 230000005540 biological transmission Effects 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 230000002255 enzymatic effect Effects 0.000 description 4
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 3
- 239000005457 ice water Substances 0.000 description 3
- 229910052700 potassium Inorganic materials 0.000 description 3
- 239000011591 potassium Substances 0.000 description 3
- 235000005979 Citrus limon Nutrition 0.000 description 2
- 201000005569 Gout Diseases 0.000 description 2
- 108010092464 Urate Oxidase Proteins 0.000 description 2
- 238000000862 absorption spectrum Methods 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000005530 etching Methods 0.000 description 2
- 230000029142 excretion Effects 0.000 description 2
- 238000004128 high performance liquid chromatography Methods 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 244000248349 Citrus limon Species 0.000 description 1
- 244000131522 Citrus pyriformis Species 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 238000013399 early diagnosis Methods 0.000 description 1
- 230000003628 erosive effect Effects 0.000 description 1
- 230000007717 exclusion Effects 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000002107 nanodisc Substances 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000002633 protecting effect Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000010189 synthetic method Methods 0.000 description 1
- 238000002371 ultraviolet--visible spectrum Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/33—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using ultraviolet light
Abstract
The uric acid detection method based on silver nanoparticle triangular plate that the invention discloses a kind of, comprising the following steps: preparation silver nanoparticle triangular plate；Sample to be tested is added in silver nanoparticle triangular plate, adds potassium rhodanide, if the absorption peak at 575nm is gone up, then illustrates to contain uric acid in sample to be tested at this point, the absorption peak at 400nm weakens.Detection method has higher sensitivity and selectivity.
Description
Technical field
The invention belongs to uric acid detection technique fields, specifically, being related to a kind of uric acid inspection based on silver nanoparticle triangular plate
Survey method.
Background technique
Under normal circumstances, about 1200 milligrams of intracorporal uric acid, newly-generated about 600 milligrams daily, while draining 600
Milligram, the state in balance.But if generation in vivo excessively has little time excretion or uric acid excretion mechanism is degenerated, then urinate in vivo
Acid is detained excessive, when blood uric acid concentration is greater than 7 milligrams/deciliter, leads to human body fluid souring, influences the normal function of human body cell
Can, gout will be caused for a long time by ignoring.In addition the excessively insufficient metabolism relative delay that also can lead to of fatigue or rest causes
Gout morbidity.
The detection of uric acid facilitates the early diagnosis of related disease.The detection method of uric acid is mainly enzymatic reagent method at present
And high performance liquid chromatography.Wherein enzymatic reagent used in enzymatic reagent method is expensive, and cost of determination is higher；Document (Uricase-
stimulated etching of silver nanoprisms for highly selective and sensitive
colorimetric detection of uric acid in human serum.Sensors and Actuators B:
Chemical 2015,221,1433-1440), used method is enzymatic reagent method, and uric acid generates under the action of uricase
Hydrogen peroxide, hydrogen peroxide cause silver nanoparticle triangular plate form to become disc from triangle silver nanoparticle triangular plate etching,
Blue shift occurs for absorption spectrum to detect to uric acid.This method main problem is to have used expensive uricase to lead
Cause the increase of testing cost.And although high performance liquid chromatography has the characteristics that easy to operate quick, the pre-treatment of sample is multiple
When incidentals, mobile phase use phosphate mostly, to chromatographic column damage it is larger.
Therefore, we are badly in need of the method for developing a kind of detection uric acid of high-efficient simple.
Summary of the invention
In view of this, the present invention provides a kind of uric acid detection method based on silver nanoparticle triangular plate.
In order to solve the above-mentioned technical problem, the uric acid detection method based on silver nanoparticle triangular plate that the invention discloses a kind of,
The following steps are included:
Step 1, preparation silver nanoparticle triangular plate: at room temperature by ultrapure water, AgNO3Solution, citric acid three sodium solution,
Hydrogen peroxide is vigorously stirred after round-bottomed flask is added；Then rapidly join NaBH4Solution continues to stir, and synthesizes colloidal sol, will synthesize
Good colloidal sol is transferred in beaker, is placed in illumination 48h under the LED light of 520nm with aluminium foil package beaker outer wall and is obtained bluish violet
Nano silver piece；Obtained silver nanoparticle triangular plate is stored loaded on brown bottle and under conditions of 4 DEG C；
Sample to be tested is added in the silver nanoparticle triangular plate that step 1 is prepared in step 2, adds potassium rhodanide, if this
When, the absorption peak at 400nm weakens, and the absorption peak at 575nm is gone up, then illustrates to contain uric acid in sample to be tested.
Optionally, the ultrapure water in the step 1: AgNO3Solution: citric acid three sodium solution: the ratio (L/ of hydrogen peroxide
It mmol/mmol/mL) is 20-28:3-8:45-55:55-65, the mass concentration of hydrogen peroxide is 25%-35%.
Optionally, the NaBH in the step 14With AgNO3The molar ratio of solution is 6:25-8:25.
Optionally, the ultrapure water in the step 1, AgNO3Solution, citric acid three sodium solution, hydrogen peroxide are vigorously stirred
Time is 1-3min；NaBH is added4Mixing time after solution is 20-30min.
Optionally, the molar ratio of potassium rhodanide and silver nanoparticle triangular plate is 35:2-35:6 in the step 2.
Compared with prior art, the present invention can be obtained including following technical effect:
1) silver nanoparticle triangular plate in the present invention is synthesized in conjunction with light radiation method using chemical method, and the synthetic method is quickly simple
Just, synthesis material is easily obtained；
2) the method for the present invention can be realized by the way that Nano silver piece to be simply mixed with determinand and potassium rhodanide, and operation is quick
The qualitative and quantitative detection to determinand can be realized by the Nano silver piece UV absorption after detection reaction in simplicity, without using
Valuable reagent or instrument；
3) detection method has higher sensitivity and selectivity.
Certainly, it implements any of the products of the present invention it is not absolutely required to while reaching all the above technical effect.
Detailed description of the invention
The drawings described herein are used to provide a further understanding of the present invention, constitutes a part of the invention, this hair
Bright illustrative embodiments and their description are used to explain the present invention, and are not constituted improper limitations of the present invention.In the accompanying drawings:
Fig. 1 be silver nanoparticle triangular plate of the present invention, silver nanoparticle triangular plate mixed with 500nM UA, silver nanoparticle triangular plate with
0.35mM KSCN effect, silver nanoparticle triangular plate are inhaled after mixing with 500nM UA with the ultraviolet-visible after 0.35mM KSCN effect
Receive spectrogram；
Fig. 2 is the UV-vis absorption spectrum figure of Nano silver piece of the present invention；
Fig. 3 is the TEM figure of Nano silver piece of the present invention；
Fig. 4 is difference KSCN concentration of the invention to Nano silver piece corrosiveness；
Fig. 5 is protecting effect of the various concentration UA of the present invention to silver nanoparticle triangular plate；Wherein, right side peak is from top to bottom successively
It is 2.00 μM corresponding, 1.00 μM, 0.90 μM, 0.80 μM, 0.70 μM, 0.60 μM, 0.50 μM, 0.40 μM, 0.30 μM, 0.20 μM,
0.10 μM, 0.05 μM, 0.01 μM, 0 μM；
Fig. 6 is the working curve of present invention detection UA；Wherein, A indicates absorption of the Nano silver piece at 400nm after reaction
Value, B indicate absorption value of the Nano silver piece at 575nm after reaction；
Fig. 7 is the selectivity of UA of the present invention.
Specific embodiment
Carry out the embodiment that the present invention will be described in detail below in conjunction with embodiment, whereby to the present invention how application technology hand
Section solves technical problem and reaches the realization process of technical effect to fully understand and implement.
The uric acid detection method based on silver nanoparticle triangular plate that the invention discloses a kind of, this method are based on potassium rhodanide
(KSCN) to silver nanoparticle triangular plate (Ag NPrs) with corrosiveness and uric acid (UA) to silver nanoparticle triangular plate have protective effect
Come what is detected indirectly to uric acid, comprising the following steps:
Step 1, preparation silver nanoparticle triangular plate (Ag NPrs): at room temperature by a certain amount of ultrapure water, AgNO3Solution,
Citric acid three sodium solution, hydrogen peroxide are vigorously stirred 1-3min after round-bottomed flask is added, wherein ultrapure water: AgNO3Solution: lemon
Lemon three sodium solutions of acid: the ratio (L/mmol/mmol/mL) of hydrogen peroxide is 20-28:3-8:45-55:55-65, hydrogen peroxide
Mass concentration is 25%-35%；Then rapidly join the NaBH of ice water Fresh4Continue to stir 20-30min, NaBH4With
AgNO3The molar ratio of solution is 6:25-8:25, obtains precursor sol；With NaBH4Addition, synthetic presoma is molten
Glue is transferred in beaker, and being placed in illumination 48h under the LED light of 520nm with aluminium foil package beaker outer wall can be obtained the silver of bluish violet
Nanometer sheet；Finally obtained silver nanoparticle triangular plate (Ag NPrs) stores loaded on brown bottle and under conditions of 4 DEG C, then to silver
Nanometer triangular plate is characterized；As shown in Fig. 2, the absorption peak is the characteristic absorption peak of silver nanoparticle triangular plate, maximum absorption band exists
At 575nm, it can be seen that by transmission electron microscope picture (Fig. 3)；It is synthesized go out silver nanoparticle triangular plate form be triangle and
Even dispersion.
Sample to be tested is added in the silver nanoparticle triangular plate that step 1 is prepared in step 2, adds potassium rhodanide,
In, the molar ratio of potassium rhodanide and silver nanoparticle triangular plate is 35:2-35:6, the purple after comparison silver nanoparticle triangular plate and KSCN effect
Outer absorption peak, if the absorption peak at 575nm is gone up, then is illustrated in sample to be tested at this point, the absorption peak at 400nm weakens
Contain uric acid.
Embodiment 1
At room temperature by a certain amount of ultrapure water (24mL), AgNO3Solution (1mL, 5mM), citric acid three sodium solution
(500 μ L, 100mM), hydrogen peroxide (60 μ L, 30wt.%) are vigorously stirred 2min after round-bottomed flask is added, and then rapidly join ice
The NaBH of water Fresh4(140 μ L, 100mM) continues to stir 25min, obtains precursor sol；With NaBH4Addition, will
Synthetic precursor sol is transferred in beaker, is placed in illumination 48h under the LED light of 520nm with aluminium foil package beaker outer wall
The Nano silver piece of bluish violet can be obtained；Finally obtained silver nanoparticle triangular plate (Ag NPrs) is loaded on brown bottle and in 4 DEG C of condition
Lower storage, then characterizes silver nanoparticle triangular plate；As shown in Fig. 2, the absorption peak is the characteristic absorption of silver nanoparticle triangular plate
Peak, maximum absorption band can be seen that at 575nm by transmission electron microscope picture (Fig. 3)；The shape of synthesized silver nanoparticle triangular plate out
State is triangle and evenly dispersed.Sample to be tested is added in the above-mentioned silver nanoparticle triangular plate being prepared, adds thiocyanic acid
The molar ratio of potassium, potassium rhodanide and silver nanoparticle triangular plate is 35:4；Ultraviolet suction after comparing silver nanoparticle triangular plate and KSCN effect
Peak is received then to illustrate to contain in sample to be tested if the absorption peak at 575nm is gone up at this point, the absorption peak at 400nm weakens
Uric acid.
Wherein, potassium rhodanide (KSCN) can corrode silver nanoparticle triangular plate at nanodiscs, as the KSCN of addition is dense
The increase of degree, maximum absorption band gradually decreases at 500nm to 700nm, final to disappear, and the peak that 330nm or so goes out also disappears therewith,
A new absorption peak is produced at 350nm to 450nm simultaneously, this absorption peak is the characteristic absorption peak of silver nanoparticle disk, this
It shows STNPs and gradually corrodes and become nano particle；As shown in figure 4, triangular plate is rotten as the amount that potassium rhodanide is added increases
The degree of erosion is also increasing, and as the amount 0.35mmol/L of addition, the extent of corrosion of silver nanoparticle triangular plate reaches maximum；By Fig. 5
It is found that the uric acid concentration with addition increases, the absorption peak at Nano silver piece 400nm is gradually decreased, and the absorption peak at 575nm
It gradually rises, absorption peak peak shape is also gradually gradually converted into the feature of silver nanoparticle triangular plate by the characteristic absorption peak shape of nanoparticle
Absorb peak shape.
It will be appreciated from fig. 6 that according to absorption value of the Nano silver piece after reaction at 400nm or the absorption value at 500nm and adding
The amount for entering uric acid fits a linear relationship；As shown in Figure 7, after according to Nano silver piece and uric acid or other material mixings
A value is obtained than the absorption value at upper 400nm with absorption value at the 575nm of the uv absorption spectra after KSCN effect, is passed through
The size of fiducial value, it can be determined that go out uric acid and the detection method is had good selectivity, detection is limited to 0.01 μM.
Embodiment 2
At room temperature by a certain amount of ultrapure water, AgNO3Round bottom is added in solution, citric acid three sodium solution, hydrogen peroxide
1min is vigorously stirred after flask, wherein ultrapure water: AgNO3Solution: citric acid three sodium solution: the ratio (L/ of hydrogen peroxide
It mmol/mmol/mL) is 20:8:45:65, the mass concentration of hydrogen peroxide is 25%；Then rapidly join ice water Fresh
NaBH4Continue to stir 30min, NaBH4With AgNO3The molar ratio of solution is 6:25, obtains precursor sol；With NaBH4Plus
Enter, synthetic precursor sol is transferred in beaker, is placed in light under the LED light of 520nm with aluminium foil package beaker outer wall
The Nano silver piece of bluish violet can be obtained according to 48h；Finally obtained silver nanoparticle triangular plate (Ag NPrs) is loaded on brown bottle and at 4 DEG C
Under conditions of store, then silver nanoparticle triangular plate is characterized；As shown in Fig. 2, the absorption peak is the spy of silver nanoparticle triangular plate
Absorption peak is levied, maximum absorption band can be seen that at 575nm by transmission electron microscope picture (Fig. 3)；Synthesized silver nanoparticle triangle out
The form of piece is triangle and evenly dispersed.Sample to be tested is added in silver nanoparticle triangular plate, adds potassium rhodanide, thiocyanic acid
The molar ratio of potassium and silver nanoparticle triangular plate is 35:2；Ultraviolet absorption peak after comparing silver nanoparticle triangular plate and KSCN effect, if this
When, the absorption peak at 400nm weakens, and the absorption peak at 575nm is gone up, then illustrates to contain uric acid in sample to be tested.
Embodiment 3
At room temperature by a certain amount of ultrapure water, AgNO3Round bottom is added in solution, citric acid three sodium solution, hydrogen peroxide
3min is vigorously stirred after flask, wherein ultrapure water: AgNO3Solution: citric acid three sodium solution: the ratio (L/ of hydrogen peroxide
It mmol/mmol/mL) is 28:3:55:55, the mass concentration of hydrogen peroxide is 35%；Then rapidly join ice water Fresh
NaBH4Continue to stir 20min, NaBH4With AgNO3The molar ratio of solution is 8:25, obtains precursor sol；With NaBH4Plus
Enter, synthetic precursor sol is transferred in beaker, is placed in light under the LED light of 520nm with aluminium foil package beaker outer wall
The Nano silver piece of bluish violet can be obtained according to 48h；Finally obtained silver nanoparticle triangular plate (Ag NPrs) is loaded on brown bottle and at 4 DEG C
Under conditions of store, then silver nanoparticle triangular plate is characterized；As shown in Fig. 2, the absorption peak is the spy of silver nanoparticle triangular plate
Absorption peak is levied, maximum absorption band can be seen that at 575nm by transmission electron microscope picture (Fig. 3)；Synthesized silver nanoparticle triangle out
The form of piece is triangle and evenly dispersed.Sample to be tested is added in silver nanoparticle triangular plate, adds potassium rhodanide, thiocyanic acid
The molar ratio of potassium and silver nanoparticle triangular plate is 35:6, the ultraviolet absorption peak after comparison silver nanoparticle triangular plate and KSCN effect, if this
When, the absorption peak at 400nm weakens, and the absorption peak at 575nm is gone up, then illustrates to contain uric acid in sample to be tested.
Above description has shown and described several preferred embodiments of invention, but as previously described, it should be understood that invention is not
It is confined to form disclosed herein, should not be regarded as an exclusion of other examples, and can be used for various other combinations, modification
And environment, and can be carried out within that scope of the inventive concept describe herein by the above teachings or related fields of technology or knowledge
Change.And changes and modifications made by those skilled in the art do not depart from the spirit and scope of invention, then it all should be in the appended power of invention
In the protection scope that benefit requires.
Claims (5)
1. a kind of uric acid detection method based on silver nanoparticle triangular plate, which comprises the following steps:
Step 1, preparation silver nanoparticle triangular plate: at room temperature by ultrapure water, AgNO3Solution, citric acid three sodium solution, peroxide
Change after round-bottomed flask is added in hydrogen and is vigorously stirred；Then rapidly join NaBH4Solution continues to stir, and synthesizes colloidal sol, will be synthetic
Colloidal sol is transferred in beaker, is placed in illumination 48h under the LED light of 520nm with aluminium foil package beaker outer wall and is obtained the silver of bluish violet
Nanometer sheet；Obtained silver nanoparticle triangular plate is stored loaded on brown bottle and under conditions of 4 DEG C；
Sample to be tested is added in the silver nanoparticle triangular plate that step 1 is prepared in step 2, adds potassium rhodanide, if at this point,
Absorption peak at 400nm weakens, and the absorption peak at 575nm is gone up, then illustrates to contain uric acid in sample to be tested.
2. uric acid detection method according to claim 1, which is characterized in that the ultrapure water in the step 1: AgNO3It is molten
Liquid: citric acid three sodium solution: the ratio (L/mmol/mmol/mL) of hydrogen peroxide is 20-28:3-8:45-55:55-65, peroxide
The mass concentration for changing hydrogen is 25%-35%.
3. uric acid detection method according to claim 1, which is characterized in that the NaBH in the step 14With AgNO3Solution
Molar ratio be 6:25-8:25.
4. uric acid detection method according to claim 1, which is characterized in that ultrapure water, AgNO in the step 13It is molten
Liquid, citric acid three sodium solution, hydrogen peroxide are vigorously stirred the time as 1-3min；NaBH is added4Mixing time after solution is 20-
30min。
5. uric acid detection method according to claim 1, which is characterized in that potassium rhodanide and silver nanoparticle in the step 2
The molar ratio of triangular plate is 35:2-35:6.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110411969A (en) * | 2019-08-02 | 2019-11-05 | 淮阴师范学院 | The method of uric acid content in determined by ultraviolet spectrophotometry poultry dung |
| CN113145170A (en) * | 2020-12-31 | 2021-07-23 | 东北电力大学 | Preparation method of visible light full-absorption saturated phosphomolybdate composite material with Keggin structure |
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