CN109266711A - The method of soybean protein isolate extraction nutrition peptide and Functional Polypeptides - Google Patents
The method of soybean protein isolate extraction nutrition peptide and Functional Polypeptides Download PDFInfo
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- CN109266711A CN109266711A CN201810999708.0A CN201810999708A CN109266711A CN 109266711 A CN109266711 A CN 109266711A CN 201810999708 A CN201810999708 A CN 201810999708A CN 109266711 A CN109266711 A CN 109266711A
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- liquid
- white liquor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
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- Proteomics, Peptides & Aminoacids (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
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Abstract
The invention discloses a kind of methods that soybean protein isolate extracts nutrition peptide and Functional Polypeptides, comprising: 1) provides soybean separation protein white liquor；2) pH value for adjusting soybean separation protein white liquor is 6-6.5, and is separated from water the protein in soybean separation protein white liquor, excludes extra waste water, the conductivity of soybean separation protein white liquor is made to be adjusted to (14-17) × 20ms；3) nutrition peptide liquid of the alkalescent protease in 56-60 DEG C of enzymatic hydrolysis 60-110-30min, after coarse filtration is digested later is added into the soybean separation protein white liquor in step 2)；4) the nutrition peptide liquid of acquisition is made into inactivation processing, removes salts substances after later handling the nutrition peptide liquid refined filtration of acquisition, the nutrition peptide is obtained after spray drying；5) albumen not digested after coarse filtration in step 3) is adjusted to the protein liquid that pH value is 7-9, alkalescent protease is added and obtains Functional Polypeptides liquid in 60 DEG C of enzymolysis processings, the Functional Polypeptides liquid inactivation of acquisition is handled later, salts substances is removed after filtering, the Functional Polypeptides is obtained after spray drying.
Description
Technical field
The present invention is more particularly directed to a kind of methods that soybean protein isolate extracts nutrition peptide and Functional Polypeptides, belong to small peptide deep processing
Technical field.
Background technique
Soybean deep processing technology constantly improves in recent years, and wherein the small peptide of soybean preparation, which has, improves the immunity of the human body,
The really supplement necessary small protein of human body enhances physical efficiency, to the cell repair of human body, until make mankind's life-time dilatation,
Control the generation of various diseases.
Summary of the invention
The main purpose of the present invention is to provide a kind of soybean protein isolate extract nutrition peptide and Functional Polypeptides method, with gram
Take the deficiencies in the prior art.
For realization aforementioned invention purpose, the technical solution adopted by the present invention includes:
The embodiment of the invention provides a kind of methods that soybean protein isolate extracts nutrition peptide and Functional Polypeptides, comprising:
1) soybean separation protein white liquor is provided, the mass percent of solid content is 2-1-5% in the soybean separation protein white liquor,
The pH value value of the soybean separating liquid is 9-10；
2) pH value for adjusting soybean separation protein white liquor is 6-6.5, and makes the protein and moisture in soybean separation protein white liquor
From excluding extra waste water, the conductivity of soybean separation protein white liquor made to be adjusted to 14-17 × 20ms；
3) alkalescent protease is added into the soybean separation protein white liquor in step 2) in 56-60 DEG C of enzymatic hydrolysis 60-110-
30min, the nutrition peptide liquid after coarse filtration is digested later；
4) the nutrition peptide liquid of acquisition is made into inactivation processing, removes salt object after later handling the nutrition peptide liquid refined filtration of acquisition
Matter obtains the nutrition peptide after spray drying；
5) albumen not digested after coarse filtration in step 3) is adjusted to the protein liquid that pH value is 7-9, alkalescent albumen is added
Enzyme obtains Functional Polypeptides liquid in 60 DEG C of enzymolysis processings, later handles the Functional Polypeptides liquid inactivation of acquisition, removes salt object after filtering
Matter obtains the Functional Polypeptides after spray drying.
Further, which comprises be added into soybean separation protein white liquor in step 2) alkalescent protease in
56-60 DEG C of first time is stirred to react 20-60min, then stops being stirred to react 10-30min；It is stirred to react 20- for the second time
Then 60min stops being stirred to react 10-30min.
Further, the alkalescent protease in soybean separation protein white liquor amount ratio be 1000-2000ml: 3-7kg.
Further, after first set reaction, the mass percent of solid content is in the soybean separation protein white liquor
3-9%, for the second time after reaction, the mass percent of solid content is 3-9% in the soybean separation protein white liquor.
Further, the method includes the nutrition peptide liquid for obtaining coarse filtration handles 7-15min in 80-100 DEG C of inactivation.
Further, which comprises adjust the mass percent of solid content in inactivation treated nutrition peptide liquid
For 1-5%, nutrition peptide liquid will be obtained later in 20-50 DEG C or less refined filtration.
Further, which comprises the solid content for being adjusted to the albumen not digested that coarse filtration in step 3) obtains
Mass percent be 3-9% protein liquid, pH value adjustment 7-9 is added at one time alkali protease and is stirred to react to solid
The mass percent of shape object is promoted to 11-5%, obtains the Functional Polypeptides liquid, later in 80-100 DEG C of processing 7-15min.
Further, which comprises adjust the mass percent of solid content in inactivation treated Functional Polypeptides liquid
For 1-5%, by the Functional Polypeptides liquid of acquisition in 20-50 DEG C or less refined filtration.
Further, the amount ratio of the protein liquid and alkali protease that do not digest is 5-10: 1-3.
Further, the mass percent of solid content is 9-10% in the nutrition peptide liquid after removing salts substances.
Further, the mass percent of solid content is 9-10% in the Functional Polypeptides liquid after removing salts substances.
Further, the molecular weight of the Functional Polypeptides is 2000-5000 dalton, and the molecular weight of the nutrition peptide is 100-
2000 dalton.
Compared with prior art, the invention has the advantages that method provided by the invention is simple, due to not using albumen point
It disembarks equipment, the protein content of peptide is up to 90% or more, and recovery rate is up to 95% or more, and production cost is decreased obviously.
Specific embodiment
In view of deficiency in the prior art, inventor is studied for a long period of time and is largely practiced, and is able to propose of the invention
Technical solution.The technical solution, its implementation process and principle etc. will be further explained as follows.
The embodiment of the invention provides a kind of methods that soybean protein isolate extracts nutrition peptide and Functional Polypeptides, comprising:
1) soybean separation protein white liquor is provided, the mass percent of solid content is 2-1-5% in the soybean separation protein white liquor,
The pH value value of the soybean separating liquid is 9-10；
2) pH value for adjusting soybean separation protein white liquor is 6-6.5, and makes the protein and moisture in soybean separation protein white liquor
From excluding extra waste water, the conductivity of soybean separation protein white liquor made to be adjusted to 14-17 × 20ms；
3) alkalescent protease is added into the soybean separation protein white liquor in step 2) in 56-60 DEG C of enzymatic hydrolysis 60-110-
30min, the nutrition peptide liquid after coarse filtration is digested later；
4) the nutrition peptide liquid of acquisition is made into inactivation processing, removes salt object after later handling the nutrition peptide liquid refined filtration of acquisition
Matter obtains the nutrition peptide after spray drying；
5) albumen not digested after coarse filtration in step 3) is adjusted to the protein liquid that pH value is 7-9, alkalescent albumen is added
Enzyme obtains Functional Polypeptides liquid in 60 DEG C of enzymolysis processings, later handles the Functional Polypeptides liquid inactivation of acquisition, removes salt object after filtering
Matter obtains the Functional Polypeptides after spray drying.
Further, which comprises be added into soybean separation protein white liquor in step 2) alkalescent protease in
56-60 DEG C of first time is stirred to react 20-60min, then stops being stirred to react 10-30min；It is stirred to react 20- for the second time
Then 60min stops being stirred to react 10-30min.
Further, the alkalescent protease in soybean separation protein white liquor amount ratio be 1000-2000ml:3-7kg.
Further, after first set reaction, the mass percent of solid content is in the soybean separation protein white liquor
3-9%, for the second time after reaction, the mass percent of solid content is 3-9% in the soybean separation protein white liquor.
Further, the method includes the nutrition peptide liquid for obtaining coarse filtration handles 7-15min in 80-100 DEG C of inactivation.
Further, which comprises adjust the mass percent of solid content in inactivation treated nutrition peptide liquid
For 1-5%, nutrition peptide liquid will be obtained later in 20-50 DEG C or less refined filtration.
Further, which comprises the solid content for being adjusted to the albumen not digested that coarse filtration in step 3) obtains
Mass percent be 3-9% protein liquid, pH value adjustment 7-9 is added at one time alkali protease and is stirred to react to solid
The mass percent of shape object is promoted to 11-5%, obtains the Functional Polypeptides liquid, later in 80-100 DEG C of processing 7-15min.
Further, which comprises adjust the mass percent of solid content in inactivation treated Functional Polypeptides liquid
For 1-5%, by the Functional Polypeptides liquid of acquisition in 20-50 DEG C or less refined filtration.
Further, the amount ratio of the protein liquid and alkali protease that do not digest is 5-10: 1-3.
Further, the mass percent of solid content is 9-10% in the nutrition peptide liquid after removing salts substances.
Further, the mass percent of solid content is 9-10% in the Functional Polypeptides liquid after removing salts substances.
Further, the molecular weight of the Functional Polypeptides is 2000-5000 dalton, and the molecular weight of the nutrition peptide is 100-
2000 dalton.
Compared with prior art, method provided by the invention is simple, due to not using Protein Separation machine equipment, the albumen of peptide
Content is up to 90% or more, and recovery rate is up to 95% or more, and production cost is decreased obviously.
Embodiment 1:
A. washing separation albumen is with material-water ratio 5Kg protein isolate: 1000 liters of water, adjustment pH value value be 9.5,35 DEG C of water temperature,
The mass percent that solid content is sufficiently stirred reaches 2.5%, is 5.3 ± 0.02 with hydrochloric acid adjustment pH value, is sufficiently stirred 5 minutes
It is by agitator tank drain valve that limpid waste water drain tank is external after being only completely separated only to water and protein naturally later, equally
Method adds 1000 liters of water stirrings again, by agitator tank drain valve handle after natural subsidence to water and albumen are completely separated after five minutes
Waste water is released, and at this moment conductivity of waste water drops to 16 × 20ms.
B. enzymolysis process, the extraction of nutrition peptide
Using the alkalescent protease of Wuxi snow plum preparation production, 57 DEG C of regulatory protein liquid temperature, pH value value is 6.5, side
Stirring side disposably puts into 2000 milliliters of protease, and by being stirred to react for the first time 40 minutes, stopping in 20 minutes is stirred to react,
It is stirred to react for the second time 40 minutes, stopping in 20 minutes is stirred to react, and amounts to reaction 2 hours, and the mass percent of solid content rises
Stop reaction when to 6.5%, after through plate cabinet filtering coarse filtration obtain nutrition peptide liquid；
Nutrition peptide liquid is kept into inactivation processing in 10 minutes through 80-100 DEG C of excess temperature, uses deionization cooling water tune after inactivation
The solid quality percentage of whole nutrition peptide liquid is 3%, and temperature is adjusted to 20-50 DEG C hereinafter, being again introduced into plate cabinet filter press essence
Filtering removes salts substances then by anionic/cationic exchange column, and handled by reverse osmosis concentration makes nutrition peptide later
The solid quality percentage of liquid is 9%, and final spray drying obtains microparticle nutrition peptide, is packaged into finished product after sterilizing.
C. the extraction of Functional Polypeptides
The albumen not digested when by plate cabinet filter press coarse filtration readjusts the matter to form that pH value value is 7-9, solid content
The protein liquid that percentage is 6% is measured, temperature is added at one time the alkalescent of Wuxi Xue Mei preparation factory production after rising to 60 DEG C
2500 milliliters of protease, the mass percent that reaction is sufficiently stirred to solid content stops reaction when rising to 13%, then directly
Inactivation processing, 80-100 DEG C of deactivation temperature is kept for 10 minutes, is with deionization cooling water week whole solid quality percentage later
3%, the filtering of plate cabinet filter press essence is reentered at 20-50 DEG C of temperature or less, and anionic/cationic exchange column is entered after filtering,
Salts substances are removed, then the mass percent of reverse osmosis concentration processing to solid content is 9%, final spray drying treatment obtains micro-
Particle Functional Polypeptides are packaged into finished product after sterilizing.
The molecular weight for the Functional Polypeptides wherein extracted is 2000-5000 dalton, and the molecular weight of nutrition peptide is the road 100-2000
Er Dun.
Method provided by the invention is simple, due to not using Protein Separation machine equipment, the protein content of peptide be up to 90% with
On, recovery rate is up to 95% or more, and production cost is decreased obviously.
It should be appreciated that the technical concepts and features of above-described embodiment only to illustrate the invention, its object is to allow be familiar with this
The personage of item technology cans understand the content of the present invention and implement it accordingly, and it is not intended to limit the scope of the present invention.It is all
Equivalent change or modification made by Spirit Essence according to the present invention, should be covered by the protection scope of the present invention.
Claims (10)
1. a kind of method that soybean protein isolate extracts nutrition peptide and Functional Polypeptides, characterized by comprising:
1) soybean separation protein white liquor is provided, the mass percent of solid content is 2-1-5% in the soybean separation protein white liquor, described
The pH value of soybean separating liquid is 9-10；
2) pH value for adjusting soybean separation protein white liquor is 6-6.5, and is separated from water the protein in soybean separation protein white liquor, is arranged
Except extra waste water, the conductivity of soybean separation protein white liquor is made to be adjusted to (14-17) × 20ms；
3) alkalescent protease is added into soybean separation protein white liquor in step 2) in 56-60 DEG C of enzymatic hydrolysis 60-110-30min,
Nutrition peptide liquid after coarse filtration is digested later；
4) the nutrition peptide liquid of acquisition is made into inactivation processing, removes salts substances after later handling the nutrition peptide liquid refined filtration of acquisition,
The nutrition peptide is obtained after spray drying；
5) by the albumen not digested after coarse filtration in step 3) be adjusted to pH value be 7-9 protein liquid, be added alkalescent protease in
60 DEG C of enzymolysis processings obtain Functional Polypeptides liquid, later handle the Functional Polypeptides liquid inactivation of acquisition, remove salts substances after filtering, spray
The Functional Polypeptides are obtained after mist is dry.
2. according to the method described in claim 1, it is characterised by comprising: being added into the soybean separation protein white liquor in step 2)
Alkalescent protease is stirred to react 20-60min in 56-60 DEG C of first time, then stops being stirred to react 10-30min；It stirs for the second time
Reaction 20-60min is mixed, then stops being stirred to react 10-30min.
3. method according to claim 1 or 2, it is characterised in that: the alkalescent protease is in soybean separation protein white liquor
Amount ratio be 1000-2000ml: 3-7kg.
4. according to the method described in claim 2, it is characterized by: after first set reaction, the soybean separation protein white liquor
The mass percent of middle solid content is 3-9%, for the second time after reaction, the quality of solid content in the soybean separation protein white liquor
Percentage is 3-9%.
5. according to the method described in claim 1, it is characterized by: the nutrition peptide liquid that coarse filtration is obtained is at 80-100 DEG C of inactivation
Manage 7-15min.
6. method according to claim 1 or 5, characterized by comprising: will solid in inactivation treated nutrition peptide liquid
The mass percent of object is adjusted to 1-5%, will obtain nutrition peptide liquid later in 20-50 DEG C or less refined filtration.
7. according to the method described in claim 1, it is characterised by comprising: the albumen not digested that coarse filtration in step 3) is obtained
The mass percent for the solid content being adjusted to is the protein liquid of 3-9%, and pH value is adjusted to 7-9, is added at one time alkali protease
And be stirred to react to the mass percent of solid content and be promoted to 11-5%, the Functional Polypeptides liquid is obtained, later at 80-100 DEG C
Manage 7-15min.
8. method according to claim 1 or claim 7, characterized by comprising: will solid in inactivation treated Functional Polypeptides liquid
The mass percent of object is adjusted to 1-5%, by the Functional Polypeptides liquid of acquisition in 20-50 DEG C or less refined filtration.
9. method according to claim 1 or claim 7, it is characterised in that: the dosage of the protein liquid and alkali protease that do not digest
Than being 5-10: 1-3.
10. according to the method described in claim 1, it is characterized by: solid content in nutrition peptide liquid after removing salts substances
Mass percent is 9-10%；And/or the mass percent of solid content is 9- in the Functional Polypeptides liquid after removing salts substances
10%；And/or the molecular weight of the Functional Polypeptides is 2000-5000 dalton, the molecular weight of the nutrition peptide is the road 100-2000
Er Dun.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810999708.0A CN109266711A (en) | 2018-08-29 | 2018-08-29 | The method of soybean protein isolate extraction nutrition peptide and Functional Polypeptides |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810999708.0A CN109266711A (en) | 2018-08-29 | 2018-08-29 | The method of soybean protein isolate extraction nutrition peptide and Functional Polypeptides |
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| CN109266711A true CN109266711A (en) | 2019-01-25 |
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| CN201810999708.0A Pending CN109266711A (en) | 2018-08-29 | 2018-08-29 | The method of soybean protein isolate extraction nutrition peptide and Functional Polypeptides |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001018229A1 (en) * | 1999-09-09 | 2001-03-15 | Farmland Industries, Inc. | Processes for making protein hydrolysates from animal peptone and for preserving mucosa |
| CN1500881A (en) * | 2002-11-12 | 2004-06-02 | 陆晓民 | Process for producing soybean protein peptide using soybean as raw material |
-
2018
- 2018-08-29 CN CN201810999708.0A patent/CN109266711A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001018229A1 (en) * | 1999-09-09 | 2001-03-15 | Farmland Industries, Inc. | Processes for making protein hydrolysates from animal peptone and for preserving mucosa |
| CN1500881A (en) * | 2002-11-12 | 2004-06-02 | 陆晓民 | Process for producing soybean protein peptide using soybean as raw material |
Non-Patent Citations (4)
| Title |
|---|
| WANG ZHENGQUAN: "Small Peptides Isolated from Enzymatic Hydrolyzate of Fermented Soybean Meal Promote Endothelium-Independent Vasorelaxation and ACE Inhibition", 《JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY》 * |
| 刘松梅: "《生物化学 第二版》", 30 June 2017, 哈尔滨工业大学出版社 * |
| 江连洲: "《酶在大豆制品中的应用》", 31 August 2015, 中国轻工业出版社 * |
| 高福成: "《食品分离重组工程技术》", 31 July 1998, 中国轻工业出版社 * |
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