CN109266333A - A kind of preparation method and application of Fluorescent silver nanocluster probe - Google Patents
A kind of preparation method and application of Fluorescent silver nanocluster probe Download PDFInfo
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- CN109266333A CN109266333A CN201811235667.4A CN201811235667A CN109266333A CN 109266333 A CN109266333 A CN 109266333A CN 201811235667 A CN201811235667 A CN 201811235667A CN 109266333 A CN109266333 A CN 109266333A
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- C09K11/00—Luminescent, e.g. electroluminescent, chemiluminescent materials
- C09K11/08—Luminescent, e.g. electroluminescent, chemiluminescent materials containing inorganic luminescent materials
- C09K11/58—Luminescent, e.g. electroluminescent, chemiluminescent materials containing inorganic luminescent materials containing copper, silver or gold
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- B22F1/00—Metallic powder; Treatment of metallic powder, e.g. to facilitate working or to improve properties
- B22F1/07—Metallic powder characterised by particles having a nanoscale microstructure
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- B—PERFORMING OPERATIONS; TRANSPORTING
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- B22F9/00—Making metallic powder or suspensions thereof
- B22F9/16—Making metallic powder or suspensions thereof using chemical processes
- B22F9/18—Making metallic powder or suspensions thereof using chemical processes with reduction of metal compounds
- B22F9/24—Making metallic powder or suspensions thereof using chemical processes with reduction of metal compounds starting from liquid metal compounds, e.g. solutions
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y20/00—Nanooptics, e.g. quantum optics or photonic crystals
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y40/00—Manufacture or treatment of nanostructures
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N21/643—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" non-biological material
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
Abstract
The invention discloses a kind of preparation method and application of Fluorescent silver nanocluster probe, belong to fluorescent nano material.Probe is the Fluorescent silver nanocluster (PVP@AgNCs) using polyvinylpyrrolidone as ligand synthesizing water-solubility.The ag nano-cluster of the hair blue-fluorescence of synthesis has higher quantum yield and lesser partial size, while finding the fluorescent quenching that iron ion can make PVP AgNCs, therefore the probe can be used for highly sensitive, highly selective detection iron ion (Fe3+).This method raw material is easy to get extensively, and cost is relatively low, easy to operate；And Fluorescent silver nanocluster probe good water solubility obtained, stability are strong, can be applied to Fe in actual water sample3+Detection, and obtain imaging of the PVP@AgNCs in human hepatoma cell.
Description
Technical field
The present invention relates to a kind of preparation method and application of Fluorescent silver nanocluster probe, belong to fluorescent nano material neck
Domain.
Background technique
Metal nanometer cluster is a kind of extra small nanoparticle of the metallic core size less than 2 nm.Recent years, high fluorescence
Intensity, high stability metal nanometer cluster by intensive report as novel fluorescence nanocluster probe, it is a variety of for detecting
Object.One obvious characteristic of metal nanometer cluster is its strong luminescence generated by light, and has size small, nontoxic, water-soluble
Good, Stocks is displaced big, good light stability and the stronger feature of anti-light bleaching power, thus it is widely emerging to cause researcher
Interest.Ag nano-cluster is increasingly becoming the important component in metal nano material, and is widely used in chemical analysis, biology biography
The research fields such as sense, bio-imaging, catalysis.
Iron (Fe) is that most wide one of metal is distributed on the earth, accounts for about the 5.1% of earth crustal mass, has in production and living
It is widely applied.Wherein, iron is also microelement necessary to life entity, key player is play in many biochemical processes, such as
Cell metabolism, enzymatic, electronics transfer, oxidation reaction, O_2 transport, synthesis of DNA and RNA etc..However, in biosystem
The content of iron must be strictly controlled, and being raised and lowered for it will all cause certain physiological barriers.The shortage of iron will lead to anaemia, intelligence
Power decline, immunity and resistance infection reduce, and a variety of diseases such as heart disease, hepatopathy, diabetes and the content of iron are excessive
It is related.The content of normal human's Iron in Serum is about 30 μm of ol/L.Regulation is drunk in China " standards for drinking water quality "
Iron content is no more than 0.3 mg/L in water.Therefore, it is desirable to which a kind of simple, high sensitivity and selective good side can be looked for
Method detects Fe3+。
Polyvinylpyrrolidone (PVP) is used as a kind of synthesizing water-solubility high-molecular compound, has excellent solubility property
And physiological compatibility.Document (Synergistic anticancer activity of fluorescent copper
nanoclusters and cisplatin delivered through a hydrogel nanocarrier, R.
Ghosh, U. Goswami, S. Sankar Ghosh, A. Paul, A. Chattopadhyay, ACS Appl.
Mater. Interfaces, 2015,7,209-222) it reports using dihydrolipoic acid as raw material, it is poly- in conjunction with biocompatibility
It closes object (polyvinylpyrrolidone) and is used as stabilizer, the synthesis of red fluorescent monodisperse CuNCs in an aqueous medium.But this method
Synthesis process needs two kinds of templates, and synthesis step is cumbersome, therefore we synthesize simple, low-cost new side there is an urgent need to a kind of
Method.
Summary of the invention
The present invention is intended to provide a kind of preparation method of Fluorescent silver nanocluster probe and the probe are detecting practical water
Iron ion (Fe in sample3+) application and cell imaging.
Since the surface area of metal nanometre cluster is high, the valence link height unsaturation of outer layer atom keeps its surface free energy very high,
This results in trend of the nano-cluster with automatic agglomeration.So stable metal nanometre cluster is made, during the preparation process,
It is generally necessary to which protective agent is added to reduce surface free energy so that nanocluster uniform in size, that stability is good is made；The present invention with
For polyvinylpyrrolidone as the silver nanoclusters that protective agent and ascorbic acid are that fluorescence is made in reducing agent, this method is easy to operate,
Cost is relatively low, and raw material is easy to get extensively.
It is using polyvinylpyrrolidone as guarantor the present invention provides a kind of preparation method of Fluorescent silver nanocluster probe
Shield agent, ascorbic acid are reducing agent, are prepared by " one kettle way ".
A kind of preparation method of Fluorescent silver nanocluster probe provided by the invention, includes the following steps:
(1) polyvinylpyrrolidone the 0.5-2.0 mL, ultrasonic 8-15 min for preparing 0.01- 0.15g/mL make it equably
It is dispersed in water；
(2) silver nitrate and 0.10-0.20 mL 80-120 of 0.10-0.20 mL, 15-25 mmol/L are added into step (1)
The ascorbic acid of mmol/L；The molar ratio of ascorbic acid and silver nitrate is 5:3-15:1；
The volume ratio of silver nitrate solution and aqueous povidone solution is 5:1-10:1；
(3) continue to stir 3.5-10 h at 30-80 DEG C；
(4) mixed solution for obtaining step (3) finally obtains Fluorescent silver nanocluster probe solution by centrifugation.
Further, the concentration of the polyvinylpyrrolidonesolution solution in step (1) is preferably 0.1 g/mL；
The molar ratio of ascorbic acid and silver nitrate is 5:1 in step (2)；
Step stirs 7 h in (3) at 70 DEG C；
Centrifugation is to be centrifuged 8-15 min with 10000-13000 r/min revolving speed in step (4).
The present invention provides the Fluorescent silver nanocluster probes using above method preparation.
The present invention provides above-mentioned Fluorescent silver nanocluster probes in water sample and organism Fe3+Application in detection.
Fluorescent silver nanocluster probe is used for water sample Fe3+In detection, detected using standard addition method；With practical water
Sample compound concentration is respectively the Fe of 5 μm of ol/L, 20 μm of ol/L, 50 μm of ol/L3+Solution takes Fluorescent silver nanocluster probe molten
100 μ L of liquid, 10 μ LFe3+Solution is added separately in 900 μ LPBS buffer solutions, and fixed excitation wavelength is 360 nm, in room
Temperature is lower to carry out fluorescence spectrum detection, and records corresponding fluorescence intensity.
Fluorescent silver nanocluster probe is used in cytotoxicity experiment, Fluorescent silver nanocluster probe solution is to human liver
The toxicity of cancer cell SMMC7721 is assessed by the MTT method of standard: by SMMC7721 cell inoculation to 96 microwell plates, then with
The Fluorescent silver nanocluster probe solution that concentration is 0-500 μ g/mL is in 37 DEG C of 24 h of common incubation；Then culture medium is abandoned,
It is added the 0.5mg/mLMTT solution of 0.1 mL into each hole, after 37 DEG C of 4 h of culture, supernatant is given up, to each Kong Zhongjia
Enter the DMSO of 150 μ L, vibrates 10 min；Absorbance of each hole at 360 nm is finally measured using microplate reader；Calculate cell
Survival rate (VR): VR=(OD experimental group/OD control group) * 100%, wherein OD experimental group has respectively indicated and do not had with OD control group
There is the absorbance in the presence of Fluorescent silver nanocluster probe solution.
Beneficial effects of the present invention:
(1) using polyvinylpyrrolidone as template, preparation method is simple, low in cost, and the silver nanoclusters of generation are with higher
Quantum yield, quantum yield 14.8%.
(2) Fluorescent silver nanocluster probe prepared by the present invention can detect the Fe in actual water sample3+；Fluorescence silver nanoparticle group
Aggregate probe has good blue luminescent properties, is used for detecting Fe in building actual water sample3+Sensing system, can be to avoid
The interference of other metal ions.
(3) Fluorescent silver nanocluster probe size made from is small, photostability is strong, good water solubility, fluorescence intensity are high, poison
Small side effects can be used for bio-imaging to Fe3+Measurement.
Detailed description of the invention
Fig. 1 is the mechanism of action schematic diagram of Fluorescent silver nanocluster probe prepared by the embodiment of the present invention 1；
Fig. 2 is the ultraviolet figure of fluorescence-that embodiment 1 prepares green fluorescence ag nano-cluster probe solution, and a is ultraviolet-visible in figure
Abosrption spectrogram, b and c are fluorescence excitation and emission peak；
Fig. 3 is the variation of fluorescence peak intensity when different cations are added in 2 Fluorescent silver nanocluster probe solution of embodiment；
Fig. 4 is the variation of 3 Fluorescent silver nanocluster probe solution of embodiment fluorescence peak intensity at various ph values；
Fig. 5 is the fluorescence peak intensity of 4 Fluorescent silver nanocluster probe solution of embodiment with the variation of response time；
Fig. 6 is 5 Fluorescent silver nanocluster probe solution of embodiment with its fluorescence peak of the variation of ionic strength (concentration of sodium chloride)
The variation of intensity；
Fig. 7 is 6 Fluorescent silver nanocluster probe solution of embodiment with Fe3+The variation of its fluorescence peak intensity of the variation of concentration；
Fig. 8 is 7 Fluorescent silver nanocluster probe solution of embodiment and concentration range is 0-100 μm of ol/L Fe3+Between it is linear
Relationship；
Fig. 9 is the survival rate of 8 Fluorescent silver nanocluster concentration and probe concentration of embodiment.
Specific embodiment
The present invention is further illustrated below by embodiment, but is not limited to following embodiment.
Embodiment 1:
Using polyvinylpyrrolidone as the preparation of the Fluorescent silver nanocluster probe of template:
(1) 1.0 mL of polyvinylpyrrolidone of 0.10 g/mL is prepared, 10 min of ultrasound are dispersed in water it uniformly；
(2) 0.15 mL, the silver nitrate of 20 mmol/L and the Vitamin C of 0.15 mL, 100 mmol/L are added into step (1)
Acid, continuing stirring reacts solution sufficiently；
(3) continue to stir 7 h at 70 DEG C；
(4) mixed solution for obtaining step (3) is centrifuged 10 min with 13000 r/min revolving speed, finally obtains fluorescence silver and receives
Rice cluster probe solution.
The mechanism of action schematic diagram of the Fluorescent silver nanocluster probe of preparation is shown in Fig. 1.
The ultraviolet figure of fluorescence-of the Fluorescent silver nanocluster probe solution of preparation is shown in Fig. 2, shows the fluorescence silver nanoparticle group of preparation
For aggregate probe under the conditions of fixed excitation wavelength is 360 nm, emission peak positions are in 440 nm or so.
Embodiment 2:
The influence of the fluorescence peak intensity for the Fluorescent silver nanocluster probe solution that metal ion prepares embodiment 1 is tested:
By metal ion Na+、K+、Al3+、Mg2+、Ca2+、Zn2+、Cu2+、Hg2+、Cd2+、Co2+、Ni2+、Mn2+、Pb2+、Fe2+、Fe3+
It is configured to the solution that concentration is 10 mmol/L, Fluorescent silver nanocluster probe solution prepared by embodiment 1 dilutes 10 times, takes
The above-mentioned solution containing different metal ions of 10 μ L is added in 1000 μ L Fluorescent silver nanocluster probe solutions after dilution, Gu
Determining excitation wavelength is 360 nm, carries out fluorescence spectrum detection at room temperature, and according to the fluorescence peak intensity of 440 nm or so, detection is not
Influence with metal ion to the fluorescence peak intensity of Fluorescent silver nanocluster probe solution.
Fig. 3 is shown in influence of the metal ion to the fluorescence peak intensity of Fluorescent silver nanocluster probe solution: exciting in 360 nm
Under, it is molten from the fluorescence intensity F and Fluorescent silver nanocluster probe of the Fluorescent silver nanocluster probe solution containing different metal ions
The fluorescence peak intensity F of liquid0Ratio (F/F0) obtain: Fe3+Variation is maximum, and the variation of other metal ions is relatively small, illustrates this
The Fluorescent silver nanocluster probe solution of invention preparation being capable of qualitative detection Fe3+。
Embodiment 3
The influence of the fluorescence intensity for the Fluorescent silver nanocluster probe solution that pH value prepares embodiment 1 is tested:
Fluorescent silver nanocluster probe solution prepared by 100 μ L embodiments 1 is added to the PBS of 900 μ L difference pH value respectively
In buffer solution, fixed excitation wavelength is 360 nm, fluorescence spectrum detection is carried out at room temperature, according to the fluorescence of 440 nm or so
Peak intensity detects influence of the pH value to the fluorescence intensity of Fluorescent silver nanocluster probe solution.
Fig. 4 is shown in influence of the pH value to the fluorescence intensity of Fluorescent silver nanocluster probe solution: under 360 nm excitation, fluorescence
After pH reaches 4, fluorescence intensity increases the fluorescence intensity of ag nano-cluster probe solution with pH, and variation is little.Thus, it examines
The environment considered in human body is 7.4, condition of the Fluorescent silver nanocluster probe solution selection prepared by the present invention in pH=7.4
Lower detection Fe3+。
Embodiment 4
The Fluorescent silver nanocluster probe solution and addition Fe that response time prepares embodiment 13+Fluorescent silver nanocluster afterwards
The influence of the fluorescence intensity of solution is tested:
Fluorescent silver nanocluster probe solution and addition Fe prepared by 100 μ L embodiments 13+Fluorescent silver nanocluster afterwards is molten
Liquid is added separately in 900 μ L PBS buffer solutions (pH=7.4), and fixed excitation wavelength is 360 nm, at room temperature 0-10
Fluorescence spectrum detection is carried out in min, according to the fluorescence peak intensity of 440 nm or so, detection time is to Fluorescent silver nanocluster probe
Solution and addition Fe3+The influence of the fluorescence intensity of Fluorescent silver nanocluster solution afterwards
Time is to Fluorescent silver nanocluster probe solution and Fe is added3+The fluorescence intensity of Fluorescent silver nanocluster solution afterwards
Fig. 5 is shown in influence: in 10 min, Fluorescent silver nanocluster probe solution and addition Fe3+Fluorescent silver nanocluster solution afterwards
Fluorescence intensity is held essentially constant, and illustrates Fe3+Response time it is very short, reaction quickly.
Embodiment 5
The influence of the fluorescence intensity for the Fluorescent silver nanocluster probe solution that ionic strength prepares embodiment 1 is tested:
By Fluorescent silver nanocluster probe solution prepared by 100 μ L embodiments 1 be added to 900 μ L PBS buffer solutions (pH=
7.4) in, fixed excitation wavelength is 360 nm, and the sodium chloride solution (0 ~ 500 mmol/L) of various concentration is added, according to
The fluorescence intensity of 440nm or so detects influence of the ionic strength to the fluorescence intensity of Fluorescent silver nanocluster probe solution.
Fig. 6 is shown in influence of the ionic strength to the fluorescence intensity of Fluorescent silver nanocluster probe solution: under 360 nm excitation,
Fluorescent silver nanocluster probe solution is in sodium chloride solution (0 ~ 500 mmol/L) range of various concentration, fluorescence intensity base
This is constant, illustrates that the anti-ion interference of Fluorescent silver nanocluster probe solution prepared by the present invention is strong.
Embodiment 6
Fluorescent silver nanocluster probe solution prepared by embodiment 1 is to Fe3+The experiment of detection:
100 μ L of Fluorescent silver nanocluster probe solution prepared by embodiment 1 is added in 900 μ LPBS buffer solutions, is added dropwise
The Fe of various concentration3+Into the solution, fixed excitation wavelength is 360 nm, fluorescence spectrum detection is carried out at room temperature, according to 440
The fluorescence intensity of nm or so detects Fe3+Influence to the fluorescence intensity of ag nano-cluster probe solution.
Fe3+Fig. 7 is shown in influence to the fluorescence intensity of Fluorescent silver nanocluster probe solution: under 360 nm excitation, fluorescence
Ag nano-cluster probe solution is in the Fe that various concentration is added3+Afterwards, fluorescence intensity is gradually reduced, and last fluorescence peak tends to be flat substantially
It is sliding；Wherein 0-150 μm of ol/L is 0,1,2,3,4,10,15,20,25,30,35,40,45,50 respectively,
55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 105, 110, 115, 120, 125130, 135,
The Fe of 140,145,150 μm of ol/L3+On the fluorescence spectra that Fluorescent silver nanocluster probe solution fluorescence intensity influences, say
Bright Fluorescent silver nanocluster probe solution prepared by the present invention can be realized to Fe3+Detection.
In addition, variation and the Fe of the fluorescence intensity of Fluorescent silver nanocluster probe solution prepared by the present invention3+Concentration be in
Linear relationship, as shown in figure 8, Fe3+Linear equation is F/F0= -0.00781[Fe3+]+0.990, the range of linearity is 1-100 μ
Mol/L, detection are limited to 384 nmol/L (signal-to-noise ratio is S/N=3).
Embodiment 7
Fluorescent silver nanocluster probe solution prepared by embodiment 1 is for Fe in actual water sample3+Test experience:
Using standard addition method for embodiment 1 prepare Fluorescent silver nanocluster probe solution in actual water sample Fe3+Detection
The experiment of application.It as shown in table 1, is respectively the Fe of 5,20,50 μm of ol/L with actual water sample compound concentration3+Solution will take reality
Apply Fluorescent silver nanocluster probe solution 100 the μ L, 10 μ LFe of the preparation of example 13+Be added separately to 900 μ LPBS buffer solutions
In, fixed excitation wavelength is 360 nm, carries out fluorescence spectrum detection at room temperature, and record corresponding fluorescence intensity.
Fe in actual water sample is calculated using the linear equation in Fig. 83+The rate of recovery.Multiple groups are measured in parallel simultaneously in this experiment
Calculate Fe3+The rate of recovery, as shown in table 1, the rate of recovery measured with this method be 97.4-103.6%, illustrate embodiment 1 make
Standby Fluorescent silver nanocluster probe solution can be used in Fe in water sample3+Detection.
Table 1 is Fluorescent silver nanocluster probe solution prepared by the present invention for Fe in actual water sample3+Detection
Embodiment 8
Fluorescent silver nanocluster probe solution prepared by embodiment 1 is used for cytotoxicity experiment
To realize cell imaging, Fluorescent silver nanocluster probe solution prepared by embodiment 1 is needed with hypotoxicity and preferable
The advantages that biocompatibility, so Fluorescent silver nanocluster probe solution passes through the toxicity of human hepatoma cell (SMMC7721)
The MTT method of standard is assessed.Simply, by the fluorescence silver in SMMC7721 cell inoculation to 96 microwell plates, then with various concentration
Nanocluster probe solution (0-500 μ g/mL) is in 37 DEG C of 24 h of common incubation.Then culture medium is abandoned, to each Kong Zhongjia
Enter the MTT solution (0.5mg/mL, solvent DMEM) of 0.1 mL, after 37 DEG C of 4 h of culture, supernatant is given up, to each Kong Zhongjia
Enter the DMSO of 150 μ L, vibrates 10 min.Absorbance (OD) of each hole at 360 nm is finally measured using microplate reader.It calculates
Cell survival rate (VR): VR=(OD experimental group/OD control group) * 100%, wherein OD experimental group has been respectively indicated with OD control group
With the absorbance in the presence of no Fluorescent silver nanocluster probe solution.
Assessment result is carried out using cytotoxicity of the MTT experiment to Fluorescent silver nanocluster probe solution and sees Fig. 9:
After the Fluorescent silver nanocluster probe solution of SMMC7721 cell and various concentration is incubated for 24 h, when Fluorescent silver nanocluster probe
When solution concentration is 200 μ g/mL, cell survival rate is still maintained at 80% or more, this illustrates that Fluorescent silver nanocluster probe is molten
Liquid has hypotoxicity, can further apply cell imaging.
Embodiment 9
Fluorescent silver nanocluster probe solution prepared by embodiment 1 is tested for cell imaging
Experiment human liver cancer cell SMMC7721 used is used and contains 10% (volume ratio) foetal bovine cleer and peaceful 1% (volume ratio) disk Buddhist nun
XiLin-streptomysin DMEM culture medium is placed in 37 DEG C, 5% CO2It is cultivated in incubator.Then 200 μ g/ are added into culture medium
ML Fluorescent silver nanocluster probe solution solution siphons away extra culture medium after 37 DEG C of 1 h of incubation, slow with the PBS of pH=7.4
Fliud flushing is washed 3 times, observes imaging contexts in fluorescence co-focusing flying-spot microscope.In addition to this, Fe is used3+At (50 μm of ol/L)
Reason contains Fluorescent silver nanocluster probe solution SMMC7721 cell, they are placed in the training containing 1 mL PBS (pH 7.4)
It supports in ware and cultivates about 2 min.Observe its imaging contexts.410-500 nm emission band is had collected in the case where excitation wavelength is 405 nm
Blue-fluorescence.
SMMC7721 cell Fluorescent silver nanocluster probe solution and contain Fe3+Fluorescent silver nanocluster probe solution mark
Note, SMMC7721 cell containing 200 μ g/mL Fluorescent silver nanocluster probe solution in cultivate 1 h after confocal fluorescent
Micro-image can be seen that SMMC7721 cellular morphology is good, is evenly distributed, and Fluorescent silver nanocluster probe solution can be with
Into in cytoplasm, bright blue-fluorescence is presented.SMMC7721 cell is containing 50 μm of ol/L Fe3+Fluorescent silver nanocluster
Confocal fluorescent imaging in probe solution is as can be seen that Fe3+Addition weaken fluorescence intensity, illustrate that the experiment can will be glimmering
Light ag nano-cluster probe solution is applied to intracellular Fe3+Detection.
Claims (10)
1. a kind of preparation method of Fluorescent silver nanocluster probe, it is characterised in that: using polyvinylpyrrolidone as protective agent,
Ascorbic acid is reducing agent, is prepared by one kettle way.
2. the preparation method of Fluorescent silver nanocluster probe according to claim 1, it is characterised in that: including walking as follows
It is rapid:
(1) polyvinylpyrrolidone the 0.5-2 mL, ultrasonic 8-12 min for preparing 0.01- 0.15g/mL divide it equably
It dissipates in water；
(2) silver nitrate and 0.10-0.20 mL 80-120 of 0.10-0.20 mL, 15-25 mmol/L are added into step (1)
The ascorbic acid of mmol/L；The molar ratio of ascorbic acid and silver nitrate is 5:3-15:1；
The volume ratio of silver nitrate solution and aqueous povidone solution is 5:1-10:1；
(3) continue to stir 3.5-10 h at 30-80 DEG C；
(4) mixed solution for obtaining step (3) finally obtains Fluorescent silver nanocluster probe solution by centrifugation.
3. the preparation method of Fluorescent silver nanocluster probe according to claim 2, it is characterised in that: in step (1)
Polyvinylpyrrolidonesolution solution concentration be 0.1 g/mL.
4. the preparation method of Fluorescent silver nanocluster probe according to claim 2, it is characterised in that: in step (2)
The molar ratio of ascorbic acid and silver nitrate is 5:1.
5. the preparation method of Fluorescent silver nanocluster probe according to claim 2, it is characterised in that: in step (3)
7 h are stirred at 70 DEG C.
6. the preparation method of Fluorescent silver nanocluster probe according to claim 2, it is characterised in that: in step (4)
Centrifugation is to be centrifuged 10 min with 10000-13000 r/min revolving speed.
7. a kind of using Fluorescent silver nanocluster probe made from the described in any item preparation methods of claim 1 ~ 6.
8. a kind of Fluorescent silver nanocluster probe as claimed in claim 7 is in water sample and organism Fe3+Application in detection.
9. application according to claim 8, it is characterised in that: Fluorescent silver nanocluster probe is used for water sample Fe3+Detection
In, it is detected using standard addition method；It is respectively 5 μm of ol/L, 20 μm of ol/L, 50 μm of ol/ with actual water sample compound concentration
The Fe of L3+Solution takes 100 μ L of Fluorescent silver nanocluster probe solution, 10 μ LFe3+Solution is added separately to 900 μ LPBS
In buffer solution, fixed excitation wavelength is 360 nm, carries out fluorescence spectrum detection at room temperature, and it is strong to record corresponding fluorescence
Degree.
10. application according to claim 8, it is characterised in that: Fluorescent silver nanocluster probe is used for cytotoxicity reality
In testing, Fluorescent silver nanocluster probe solution assesses the toxicity of human hepatoma cell SMMC7721 by the MTT method of standard:
By in SMMC7721 cell inoculation to 96 microwell plates, then the Fluorescent silver nanocluster probe solution for being 0-500 μ g/mL with concentration
It is incubated for jointly for 24 hours at 37 DEG C；Then culture medium is abandoned, is added the 0.5 mg/mLMTT solution of 0.1 mL into each hole, 37
After DEG C 4 h of culture, supernatant is given up, is added the DMSO of 150 μ L into each hole, vibrates 10 min；Finally use microplate reader
Measure absorbance of each hole at 360 nm；Cell survival rate (VR): VR=(OD experimental group/OD control group) * 100% is calculated,
Wherein, OD experimental group and OD control group respectively indicate and without the absorbances in the presence of Fluorescent silver nanocluster probe solution.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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| CN111208099A (en) * | 2020-01-08 | 2020-05-29 | 同济大学 | Silver nano-cluster fluorescent probe, preparation method thereof and application thereof in detection of active oxygen species |
| CN112175608A (en) * | 2020-10-22 | 2021-01-05 | 江南大学 | Blue fluorescent silver nanocluster and preparation method and application thereof |
| CN112175608B (en) * | 2020-10-22 | 2021-11-02 | 江南大学 | Blue fluorescent silver nanocluster and preparation method and application thereof |
| CN113369489A (en) * | 2021-05-13 | 2021-09-10 | 山西大学 | Luminescent silver nanocluster and preparation method and application thereof |
| CN113369489B (en) * | 2021-05-13 | 2023-03-07 | 山西大学 | Luminescent silver nanocluster and preparation method and application thereof |
| CN114790216A (en) * | 2022-03-09 | 2022-07-26 | 山西大学 | Red fluorescent silver nanocluster and preparation method and application thereof |
| CN114790216B (en) * | 2022-03-09 | 2023-09-22 | 山西大学 | Red fluorescent silver nanocluster and preparation method and application thereof |
| CN114672305A (en) * | 2022-03-23 | 2022-06-28 | 中电华创电力技术研究有限公司 | Preparation method of copper nanocluster fluorescent probe and detection method of hypochlorite in water environment |
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| CN115780823B (en) * | 2022-12-16 | 2024-03-15 | 琼台师范学院 | Preparation method and application of fluorescent silver nanocluster |
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