CN109251280A - A kind of preparation method with anticoagulant property biology 3D printing material - Google Patents
A kind of preparation method with anticoagulant property biology 3D printing material Download PDFInfo
- Publication number
- CN109251280A CN109251280A CN201810784599.0A CN201810784599A CN109251280A CN 109251280 A CN109251280 A CN 109251280A CN 201810784599 A CN201810784599 A CN 201810784599A CN 109251280 A CN109251280 A CN 109251280A
- Authority
- CN
- China
- Prior art keywords
- parts
- printing material
- preparation
- anticoagulant property
- property biology
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
- C08F283/00—Macromolecular compounds obtained by polymerising monomers on to polymers provided for in subclass C08G
- C08F283/02—Macromolecular compounds obtained by polymerising monomers on to polymers provided for in subclass C08G on to polycarbonates or saturated polyesters
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B33—ADDITIVE MANUFACTURING TECHNOLOGY
- B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
- B33Y70/00—Materials specially adapted for additive manufacturing
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
- C08F251/00—Macromolecular compounds obtained by polymerising monomers on to polysaccharides or derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
- C08F289/00—Macromolecular compounds obtained by polymerising monomers on to macromolecular compounds not provided for in groups C08F251/00 - C08F287/00
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
- C08K13/00—Use of mixtures of ingredients not covered by one single of the preceding main groups, each of these compounds being essential
- C08K13/02—Organic and inorganic ingredients
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
- C08K3/00—Use of inorganic substances as compounding ingredients
- C08K3/18—Oxygen-containing compounds, e.g. metal carbonyls
- C08K3/20—Oxides; Hydroxides
- C08K3/22—Oxides; Hydroxides of metals
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
- C08K3/00—Use of inorganic substances as compounding ingredients
- C08K3/34—Silicon-containing compounds
- C08K3/346—Clay
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
- C08K5/00—Use of organic ingredients
- C08K5/04—Oxygen-containing compounds
- C08K5/09—Carboxylic acids; Metal salts thereof; Anhydrides thereof
- C08K5/098—Metal salts of carboxylic acids
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
- C08K5/00—Use of organic ingredients
- C08K5/04—Oxygen-containing compounds
- C08K5/10—Esters; Ether-esters
- C08K5/101—Esters; Ether-esters of monocarboxylic acids
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
- C08K3/00—Use of inorganic substances as compounding ingredients
- C08K3/18—Oxygen-containing compounds, e.g. metal carbonyls
- C08K3/20—Oxides; Hydroxides
- C08K3/22—Oxides; Hydroxides of metals
- C08K2003/2227—Oxides; Hydroxides of metals of aluminium
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Manufacturing & Machinery (AREA)
- Materials Engineering (AREA)
- Dispersion Chemistry (AREA)
- Materials For Medical Uses (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
The invention discloses a kind of preparation method with anticoagulant property biology 3D printing material, which passes through high speed ball milling for raw materials such as polycarbonate, ethylene carbonate, kaolin, aluminium oxide, chlorinated paraffin, butyl stearate, sulfonation glucan, hyaluronic acid, ethylene glycol diglycidylether, lauric acid zinc soaps respectively, sieving sorts, vacuum nitrogen is catalyzed reaction, vacuum filtration, wash repeatedly, drying, sonic oscillation disperses, cryospray drying and other steps are prepared with anticoagulant property biology 3D printing material.What is be prepared has anticoagulant property biology 3D printing material, and cost of material is low, light hardness is big, has significant anticoagulant functions, is suitable for the application of a variety of surgical instrument consumptive materials, hemostatic device etc..
Description
Technical field
The present invention relates to this technical fields of 3D printing material, are related specifically to a kind of with the 3D printing of anticoagulant property biology
The preparation method of material.
Background technique
3D printing (3D printing) is one kind based on digital model file, with flow-like, powdered, silk
(stick) shape etc. is curable, bonds, alloying material, and the technology of object is constructed by way of successively solidifying, bonding, fuse.Often
Be used for modeling in fields such as mold manufacture, industrial designs, after be gradually available for the direct manufactures of some products, having had makes
The components made of the printing of this technology.The technology is in jewelry, footwear, industrial design, building, engineering and construction (AEC), vapour
Vehicle, aerospace, dentistry and medical industries, education, GIS-Geographic Information System, civil engineering, gun and other field are all
Using.3D printing technique appears in mid-term the 1990s, actually utilizes the newest of the technologies such as photocuring and paper layer be folded
Rapid molding device.It is essentially identical with common print working principle, and printer is provided with liquid or powder etc. " printed material ",
After being connect with computer, is controlled by computer and " printed material " is stacked up from level to level, finally the blueprint on computer is become
It is in kind.This printing technique is known as 3D three-dimensional printing technology.Traditional manufacture, which generally requires, to be cut raw material or is drilled, i.e.,
Subtract material manufacture, can be mass-produced；3D printing is that material is stacked to bonding, fusion, i.e. increasing material manufacturing from level to level；It can be achieved quick
Individual character manufacturing can produce the impossible shape of traditional manufacture.Based on biologic applications be directed toward 3D printing technique,
Printed material composition has more particular/special requirements compared with other field 3D printing material.Such as high temperature sintering, organic solvent,
The conditions such as ultraviolet irradiation and crosslinking agent must often avoid as far as possible during biological 3D printing.Specifically, according to application purpose
Must consider claimed below: a. printability, what the performances such as viscosity, rheology and the gelation of material directly influenced 3D printing can
Operability, it is resolved that print the room and time resolution ratio of product.B. biocompatibility includes biological safety and biological function
Property material does not require nothing more than very low toxicity and does not cause any adverse reaction of body, and material is required specifically to answer
With the corresponding function of middle excitation body.C. degradability includes the degradation speed controllability of material, with regeneration speed
With property, catabolite safety, the swelling of material and shrinkage character etc..D. structure and mechanical performance, the material of 3D printing is often
Have the function of supporting cell and tissue three-dimensional structure.For specific organization type, from skin, muscle, cartilage to os osseum,
Must have different mechanical strength and micro-structure, the especially hole of material after file printing.E. bionics characteristic, material are imitative
Raw characteristic of learning is conducive to stimulate cellular response.Biological active component is mixed in biomaterial to glue endogenous or foreign cell
Attached, migration, proliferation and functional expression generate positive effect.In addition, the modification of material surface property such as chemical group, roughness,
What hydrophilic and hydrophobic, micro nano structure etc. directly influenced cell sprawls shape, atomization, movement, orientation, the group of cytoskeleton
Dress, the even associated signal paths of cell interior.
Summary of the invention
In order to solve the above-mentioned technical problem, the invention discloses a kind of preparations with anticoagulant property biology 3D printing material
Method, the technique gather polycarbonate, ethylene carbonate, kaolin, aluminium oxide, chlorinated paraffin, butyl stearate, sulfonation Portugal
The raw materials such as sugar, hyaluronic acid, ethylene glycol diglycidylether, lauric acid zinc soap pass through high speed ball milling, sieving sorting, vacuum respectively
Nitrogen catalysis reaction, vacuum filtration, tool is prepared in washing, dry, sonic oscillation dispersion, cryospray drying and other steps repeatedly
There is anticoagulant property biology 3D printing material.What is be prepared has anticoagulant property biology 3D printing material, and cost of material is low, matter
Light hardness is big, has significant anticoagulant functions, is suitable for the application of a variety of surgical instrument consumptive materials, hemostatic device etc..
Technical solution: to solve the above-mentioned problems, the invention discloses one kind to have anticoagulant property biology 3D printing material
Preparation method, comprising the following steps:
(1) by 12-15 parts of polycarbonate, 15-20 parts of ethylene carbonate, 2-7 parts of kaolin, 1-4 parts of aluminium oxide, chlorinated paraffin 2-
6 parts, 2-4 parts of butyl stearate are added in high speed ball mill, and ratio of grinding media to material 99:4 carries out ball milling, obtained mixture of powders sieving
Sorting, it is spare；
(2) the sieving powder of step (1) is added in vacuum reaction kettle, 2-5 parts of sulfonation glucan, hyaluronic acid 1-3 is added
Part, 2-5 parts of ethylene glycol diglycidylether, 1-4 parts of lauric acid zinc soap, 1-3 parts of initiator, 2-3 parts of auxiliary agent, are heated to 70-75
It is passed through nitrogen after DEG C and excludes oxygen, then temperature rises to 80-85 DEG C again, continues insulation reaction 6-10h, after reaction in furnace
Air pressure returns back to normal pressure, and reactant cooling is spare；
(3) vacuum filtration machine is added in the reactant of step (2), with sterile water washing 3 times, it is dry that suction filtration product is placed in 65 DEG C of vacuum
Dry 40-60min in dry case；
(4) it by 3-5 parts of the dried object of step (3), dispersing agent, is added in ultrasonator, carries out ultrasonic disperse；
(5) the ultrasonic disperse product of step (4) is injected into cryospray drying machine, carries out powdered material, drying, collection, packet
It fills, get product.
Preferably, the rotational speed of ball-mill in the step (1) is 3000r/min, Ball-milling Time 30-50min.
Preferably, the mesh size of crossing in the step (1) is 5000 mesh.
Preferably, the initiator in the step (2) is selected from azodiisobutyronitrile, azobisisoheptonitrile, benzoyl peroxide first
One or more of acyl, lauroyl peroxide.
Preferably, the auxiliary agent in the step (2) is in stearoylketene amine, oleamide, erucyl amide, zinc stearate
It is one or more of.
Preferably, the nitrogen pressure in the step (2) is 5MPa.
Preferably, the vacuum filtration pressure in the step (3) is 5*10-8Pa。
Preferably, the dispersing agent in the step (4) is in sodium pyrophosphate, more sodium metaphosphates, potassium citrate, sodium metasilicate
One or more.
Preferably, the sonic oscillation power in the step (4) is 500W, ultrasonic time 90min.
Preferably, it is -25 DEG C that the cryospray drying parameter in the step (5), which is cryogenic temperature, and condenser temperature is -75
DEG C, atomisation pressure 5BAR.
Compared with prior art, the present invention has the advantages that:
(1) a kind of preparation method with anticoagulant property biology 3D printing material of the invention by polycarbonate, ethylene carbonate,
Kaolin, aluminium oxide, chlorinated paraffin, butyl stearate, sulfonation glucan, hyaluronic acid, ethylene glycol diglycidylether, laurel
The raw materials such as sour zinc soap pass through high speed ball milling respectively, sieving sorting, vacuum nitrogen catalysis reaction, vacuum filtration, wash repeatedly, is dry
Dry, sonic oscillation dispersion, cryospray drying and other steps are prepared with anticoagulant property biology 3D printing material.It is prepared
Have anticoagulant property biology 3D printing material, cost of material is low, light hardness is big, have significant anticoagulant functions, fit
Together in the application of a variety of surgical instrument consumptive materials, hemostatic device etc..
(2) of the invention there is anticoagulant property biology 3D printing material feedstock to be easy to get, simple process, be suitable for large-scale industry
Change and uses, it is practical.
Specific embodiment
Embodiment 1
(1) by 12 parts of polycarbonate, 15 parts of ethylene carbonate, 2 parts of kaolin, 1 part of aluminium oxide, 2 parts of chlorinated paraffin, stearic acid fourth
It 2 parts of ester, is added in high speed ball mill, rotational speed of ball-mill 3000r/min, Ball-milling Time 30min, ratio of grinding media to material 99:4 carries out ball
Mill, obtained mixture of powders sieving sorting, crossing mesh size is 5000 mesh, spare；
(2) the sieving powder of step (1) is added in vacuum reaction kettle, 2 parts of sulfonation glucan, 1 part of hyaluronic acid, second is added
2 parts of Hexanediol diglycidyl ether, 1 part of lauric acid zinc soap, 1 part of azodiisobutyronitrile, 2 parts of stearoylketene amine, after being heated to 70-75 DEG C
It is passed through nitrogen and excludes oxygen, nitrogen pressure 5Mpa, then temperature rises to 80-85 DEG C again, continues insulation reaction 6h, reaction knot
Air pressure returns back to normal pressure in Shu Houlu, and reactant cooling is spare；
(3) vacuum filtration machine is added in the reactant of step (2), vacuum filtration pressure is 5*10-8Pa, with sterile water washing 3
It is secondary, it filters product and is placed in 65 DEG C of vacuum ovens dry 40min；
(4) it by 3 parts of the dried object of step (3), sodium pyrophosphate, is added in ultrasonator, carries out ultrasonic disperse, sonic oscillation function
Rate is 500W, ultrasonic time 90min；
(5) the ultrasonic disperse product of step (4) is injected into cryospray drying machine, cryospray drying parameter is cryogenic temperature
Be -25 DEG C, condenser temperature be -75 DEG C, atomisation pressure 5BAR, carry out powdered material, drying, collection, packaging, up at
Product.
Embodiment 2
(1) by 13 parts of polycarbonate, 17 parts of ethylene carbonate, 3 parts of kaolin, 2 parts of aluminium oxide, 4 parts of chlorinated paraffin, stearic acid fourth
It 3 parts of ester, is added in high speed ball mill, rotational speed of ball-mill 3000r/min, Ball-milling Time 40min, ratio of grinding media to material 99:4 carries out ball
Mill, obtained mixture of powders sieving sorting, crossing mesh size is 5000 mesh, spare；
(2) the sieving powder of step (1) is added in vacuum reaction kettle, 3 parts of sulfonation glucan, 2 parts of hyaluronic acid, second is added
3 parts of Hexanediol diglycidyl ether, 2 parts of lauric acid zinc soap, 2 parts of azobisisoheptonitrile, 2 parts of oleamide, after being heated to 70-75 DEG C
It is passed through nitrogen and excludes oxygen, nitrogen pressure 5Mpa, then temperature rises to 80-85 DEG C again, continues insulation reaction 7h, reaction knot
Air pressure returns back to normal pressure in Shu Houlu, and reactant cooling is spare；
(3) vacuum filtration machine is added in the reactant of step (2), vacuum filtration pressure is 5*10-8Pa, with sterile water washing 3
It is secondary, it filters product and is placed in 65 DEG C of vacuum ovens dry 50min；
(4) it by 4 parts of the dried object of step (3), more sodium metaphosphates, is added in ultrasonator, carries out ultrasonic disperse, sonic oscillation
Power is 500W, ultrasonic time 90min；
(5) the ultrasonic disperse product of step (4) is injected into cryospray drying machine, cryospray drying parameter is cryogenic temperature
Be -25 DEG C, condenser temperature be -75 DEG C, atomisation pressure 5BAR, carry out powdered material, drying, collection, packaging, up at
Product.
Embodiment 3
(1) by 14 parts of polycarbonate, 19 parts of ethylene carbonate, 6 parts of kaolin, 3 parts of aluminium oxide, 5 parts of chlorinated paraffin, stearic acid fourth
It 3 parts of ester, is added in high speed ball mill, rotational speed of ball-mill 3000r/min, Ball-milling Time 45min, ratio of grinding media to material 99:4 carries out ball
Mill, obtained mixture of powders sieving sorting, crossing mesh size is 5000 mesh, spare；
(2) the sieving powder of step (1) is added in vacuum reaction kettle, sulfonation bextran 45 part, 3 parts of hyaluronic acid, second is added
4 parts of Hexanediol diglycidyl ether, 3 parts of lauric acid zinc soap, 2 parts of benzoyl peroxide, 3 parts of erucyl amide, after being heated to 70-75 DEG C
It is passed through nitrogen and excludes oxygen, nitrogen pressure 5Mpa, then temperature rises to 80-85 DEG C again, continues insulation reaction 9h, reaction knot
Air pressure returns back to normal pressure in Shu Houlu, and reactant cooling is spare；
(3) vacuum filtration machine is added in the reactant of step (2), vacuum filtration pressure is 5*10-8Pa, with sterile water washing 3
It is secondary, it filters product and is placed in 65 DEG C of vacuum ovens dry 45min；
(4) it by 4 parts of the dried object of step (3), potassium citrate, is added in ultrasonator, carries out ultrasonic disperse, sonic oscillation function
Rate is 500W, ultrasonic time 90min；
(5) the ultrasonic disperse product of step (4) is injected into cryospray drying machine, cryospray drying parameter is cryogenic temperature
Be -25 DEG C, condenser temperature be -75 DEG C, atomisation pressure 5BAR, carry out powdered material, drying, collection, packaging, up at
Product.
Embodiment 4
(1) by 15 parts of polycarbonate, 20 parts of ethylene carbonate, 7 parts of kaolin, 4 parts of aluminium oxide, 6 parts of chlorinated paraffin, stearic acid fourth
It 4 parts of ester, is added in high speed ball mill, rotational speed of ball-mill 3000r/min, Ball-milling Time 50min, ratio of grinding media to material 99:4 carries out ball
Mill, obtained mixture of powders sieving sorting, crossing mesh size is 5000 mesh, spare；
(2) the sieving powder of step (1) is added in vacuum reaction kettle, 5 parts of sulfonation glucan, 3 parts of hyaluronic acid, second is added
5 parts of Hexanediol diglycidyl ether, 4 parts of lauric acid zinc soap, 3 parts of lauroyl peroxide, 3 parts of zinc stearate, after being heated to 70-75 DEG C
It is passed through nitrogen and excludes oxygen, nitrogen pressure 5Mpa, then temperature rises to 80-85 DEG C again, continues insulation reaction 10h, reaction
After in furnace air pressure return back to normal pressure, reactant cooling is spare；
(3) vacuum filtration machine is added in the reactant of step (2), vacuum filtration pressure is 5*10-8Pa, with sterile water washing 3
It is secondary, it filters product and is placed in 65 DEG C of vacuum ovens dry 60min；
(4) it by 5 parts of the dried object of step (3), sodium metasilicate, is added in ultrasonator, carries out ultrasonic disperse, sonic oscillation power
For 500W, ultrasonic time 90min；
(5) the ultrasonic disperse product of step (4) is injected into cryospray drying machine, cryospray drying parameter is cryogenic temperature
Be -25 DEG C, condenser temperature be -75 DEG C, atomisation pressure 5BAR, carry out powdered material, drying, collection, packaging, up at
Product.
Comparative example 1
(1) it by 12 parts of polycarbonate, 15 parts of ethylene carbonate, 2 parts of kaolin, 1 part of aluminium oxide, 2 parts of butyl stearate, is added high
In fast ball mill, rotational speed of ball-mill 3000r/min, Ball-milling Time 30min, ratio of grinding media to material 99:4 carry out ball milling, obtained powder
Mixture sieving sorting, crossing mesh size is 5000 mesh, spare；
(2) the sieving powder of step (1) is added in vacuum reaction kettle, 1 part of hyaluronic acid, ethylene glycol diglycidyl is added
2 parts of ether, 1 part of lauric acid zinc soap, 1 part of azodiisobutyronitrile, 2 parts of stearoylketene amine are passed through nitrogen and exclude oxygen after being heated to 70-75 DEG C
Gas, nitrogen pressure 5Mpa, then temperature rises to 80-85 DEG C again, continues insulation reaction 6h, and air pressure is returned in furnace after reaction
Again to normal pressure, reactant cooling is spare；
(3) vacuum filtration machine is added in the reactant of step (2), vacuum filtration pressure is 5*10-8Pa, with sterile water washing 3
It is secondary, it filters product and is placed in 65 DEG C of vacuum ovens dry 40min；
(4) it by 3 parts of the dried object of step (3), sodium pyrophosphate, is added in ultrasonator, carries out ultrasonic disperse, sonic oscillation function
Rate is 500W, ultrasonic time 90min；
(5) the ultrasonic disperse product of step (4) is injected into cryospray drying machine, cryospray drying parameter is cryogenic temperature
Be -25 DEG C, condenser temperature be -75 DEG C, atomisation pressure 5BAR, carry out powdered material, drying, collection, packaging, up at
Product.
Comparative example 2
(1) by 15 parts of polycarbonate, 20 parts of ethylene carbonate, 7 parts of kaolin, 4 parts of aluminium oxide, 6 parts of chlorinated paraffin, stearic acid fourth
It 4 parts of ester, is added in high speed ball mill, rotational speed of ball-mill 3000r/min, Ball-milling Time 50min, ratio of grinding media to material 99:4 carries out ball
Mill, obtained mixture of powders sieving sorting, crossing mesh size is 5000 mesh, spare；
(2) the sieving powder of step (1) is added in vacuum reaction kettle, 5 parts of sulfonation glucan, 3 parts of hyaluronic acid, second is added
5 parts of Hexanediol diglycidyl ether, 4 parts of lauric acid zinc soap, 3 parts of lauroyl peroxide, 3 parts of zinc stearate, after being heated to 70-75 DEG C
It is passed through nitrogen and excludes oxygen, nitrogen pressure 5Mpa, then temperature rises to 80-85 DEG C again, continues insulation reaction 10h, reaction
After in furnace air pressure return back to normal pressure, reactant cooling is spare；
(3) it by 5 parts of the dried object of step (2), sodium metasilicate, is added in ultrasonator, carries out ultrasonic disperse, sonic oscillation power
For 500W, ultrasonic time 90min；
(4) the ultrasonic disperse product of step (3) is injected into cryospray drying machine, cryospray drying parameter is cryogenic temperature
Be -25 DEG C, condenser temperature be -75 DEG C, atomisation pressure 5BAR, carry out powdered material, drying, collection, packaging, up at
Product.
Will made from embodiment 1-4 and comparative example 1-2 have anticoagulant property biology 3D printing material carry out respectively density,
This several blood coagulation test, compression strength performance tests, test result are shown in Table 1.
Table 1
| Density/g.cm-1 | 30min is tested in blood coagulation | Compression strength/Mpa | |
| Embodiment 1 | 1.253 | Without blood coagulation | 625 |
| Embodiment 2 | 1.312 | Without blood coagulation | 620 |
| Embodiment 3 | 1.273 | Without blood coagulation | 630 |
| Embodiment 4 | 1.280 | Without blood coagulation | 635 |
| Comparative example 1 | 1.783 | Without blood coagulation | 265 |
| Comparative example 2 | 1.906 | Without blood coagulation | 315 |
A kind of preparation method with anticoagulant property biology 3D printing material of the invention is by polycarbonate, ethylene carbonate, height
Ridge soil, aluminium oxide, chlorinated paraffin, butyl stearate, sulfonation glucan, hyaluronic acid, ethylene glycol diglycidylether, lauric acid
The raw materials such as zinc soap pass through respectively high speed ball milling, sieving sorting, vacuum nitrogen catalysis reaction, vacuum filtration, wash repeatedly, be dry,
Sonic oscillation dispersion, cryospray drying and other steps are prepared with anticoagulant property biology 3D printing material.It is prepared
With anticoagulant property biology 3D printing material, cost of material is low, light hardness is big, has significant anticoagulant functions, is suitble to
In the application of a variety of surgical instrument consumptive materials, hemostatic device etc..Of the invention has anticoagulant property biology 3D printing material former
Material be easy to get, simple process, be suitable for heavy industrialization use, it is practical.
The above description is only an embodiment of the present invention, is not intended to limit the scope of the invention, all to utilize this hair
Equivalent structure or equivalent flow shift made by bright description is applied directly or indirectly in other relevant technology necks
Domain is included within the scope of the present invention.
Claims (10)
1. a kind of preparation method with anticoagulant property biology 3D printing material, which comprises the following steps:
(1) by 12-15 parts of polycarbonate, 15-20 parts of ethylene carbonate, 2-7 parts of kaolin, 1-4 parts of aluminium oxide, chlorinated paraffin 2-
6 parts, 2-4 parts of butyl stearate are added in high speed ball mill, and ratio of grinding media to material 99:4 carries out ball milling, obtained mixture of powders sieving
Sorting, it is spare；
(2) the sieving powder of step (1) is added in vacuum reaction kettle, 2-5 parts of sulfonation glucan, hyaluronic acid 1-3 is added
Part, 2-5 parts of ethylene glycol diglycidylether, 1-4 parts of lauric acid zinc soap, 1-3 parts of initiator, 2-3 parts of auxiliary agent, are heated to 70-75
It is passed through nitrogen after DEG C and excludes oxygen, then temperature rises to 80-85 DEG C again, continues insulation reaction 6-10h, after reaction in furnace
Air pressure returns back to normal pressure, and reactant cooling is spare；
(3) vacuum filtration machine is added in the reactant of step (2), with sterile water washing 3 times, it is dry that suction filtration product is placed in 65 DEG C of vacuum
Dry 40-60min in dry case；
(4) it by 3-5 parts of the dried object of step (3), dispersing agent, is added in ultrasonator, carries out ultrasonic disperse；
(5) the ultrasonic disperse product of step (4) is injected into cryospray drying machine, carries out powdered material, drying, collection, packet
It fills, get product.
2. the preparation method according to claim 1 with anticoagulant property biology 3D printing material, which is characterized in that described
Rotational speed of ball-mill in step (1) is 3000r/min, Ball-milling Time 30-50min.
3. the preparation method according to claim 1 with anticoagulant property biology 3D printing material, which is characterized in that described
Mesh size of crossing in step (1) is 5000 mesh.
4. the preparation method according to claim 1 with anticoagulant property biology 3D printing material, which is characterized in that described
Initiator in step (2) is in azodiisobutyronitrile, azobisisoheptonitrile, benzoyl peroxide, lauroyl peroxide
It is one or more of.
5. the preparation method according to claim 1 with anticoagulant property biology 3D printing material, which is characterized in that described
Auxiliary agent in step (2) is selected from one or more of stearoylketene amine, oleamide, erucyl amide, zinc stearate.
6. the preparation method according to claim 1 with anticoagulant property biology 3D printing material, which is characterized in that described
Nitrogen pressure in step (2) is 5MPa.
7. the preparation method according to claim 1 with anticoagulant property biology 3D printing material, which is characterized in that described
Vacuum filtration pressure in step (3) is 5*10-8Pa。
8. the preparation method according to claim 1 with anticoagulant property biology 3D printing material, which is characterized in that described
Dispersing agent in step (4) is selected from one or more of sodium pyrophosphate, more sodium metaphosphates, potassium citrate, sodium metasilicate.
9. the preparation method according to claim 1 with anticoagulant property biology 3D printing material, which is characterized in that described
Sonic oscillation power in step (4) is 500W, ultrasonic time 90min.
10. the preparation method according to claim 1 with anticoagulant property biology 3D printing material, which is characterized in that institute
It is -25 DEG C that state the cryospray drying parameter in step (5), which be cryogenic temperature, and condenser temperature is -75 DEG C, and atomisation pressure is
5BAR。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810784599.0A CN109251280A (en) | 2018-07-17 | 2018-07-17 | A kind of preparation method with anticoagulant property biology 3D printing material |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810784599.0A CN109251280A (en) | 2018-07-17 | 2018-07-17 | A kind of preparation method with anticoagulant property biology 3D printing material |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109251280A true CN109251280A (en) | 2019-01-22 |
Family
ID=65048631
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810784599.0A Pending CN109251280A (en) | 2018-07-17 | 2018-07-17 | A kind of preparation method with anticoagulant property biology 3D printing material |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109251280A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109771701A (en) * | 2019-02-11 | 2019-05-21 | 赵延延 | A kind of preparation method of soft biological 3D printing material |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108136496A (en) * | 2015-10-09 | 2018-06-08 | 赛丹思科大学 | 3d printing raw material and its application |
-
2018
- 2018-07-17 CN CN201810784599.0A patent/CN109251280A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108136496A (en) * | 2015-10-09 | 2018-06-08 | 赛丹思科大学 | 3d printing raw material and its application |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109771701A (en) * | 2019-02-11 | 2019-05-21 | 赵延延 | A kind of preparation method of soft biological 3D printing material |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Zhao et al. | Fabrication of scaffolds in tissue engineering: A review | |
| Shi et al. | Cryogenic free-form extrusion bioprinting of decellularized small intestinal submucosa for potential applications in skin tissue engineering | |
| Jiang et al. | Preparation of cellulose nanocrystal/oxidized dextran/gelatin (CNC/OD/GEL) hydrogels and fabrication of a CNC/OD/GEL scaffold by 3D printing | |
| Jiang et al. | Lignin‐based materials for additive manufacturing: chemistry, processing, structures, properties, and applications | |
| Tian et al. | Applications of adhesives in textiles: A review | |
| CN109251280A (en) | A kind of preparation method with anticoagulant property biology 3D printing material | |
| FI128467B (en) | Three dimensional printing with biomaterial | |
| Deng et al. | Simultaneous enhancement of elasticity and strength of Al2O3-based ceramics body from cellulose nanocrystals via gel-casting process | |
| Hafez et al. | Comprehensive insight into foams made of thermomechanical pulp fibers and cellulose nanofibrils via microwave radiation | |
| Chen et al. | 3D bioprinting of complex biological structures with tunable elastic modulus and porosity using freeform reversible embedding of suspended hydrogels | |
| CN109052368A (en) | A method of carbon quantum dot film being prepared using hydrothermal technique using garlic as raw material | |
| SHAO et al. | Nanocomposite “Xuan Paper” made from ultralong hydroxyapatite nanowires and cellulose fibers and its anti-mildew properties | |
| Madbouly | Bio-based castor oil and lignin sulphonate: aqueous dispersions and shape-memory films | |
| CN108553689B (en) | Silk fibroin porous microsphere with nanofiber microstructure and preparation method thereof | |
| CN108485405A (en) | A kind of preparation method of polyvinyl alcohol Stripable paint | |
| CN106519529A (en) | Medical biomaterial with high toughness and high surface hardness, and preparation method thereof | |
| Schiraldi | Green polymer aerogels | |
| CN108841161A (en) | A kind of preparation method of safe and non-stimulating biometric print material | |
| CN109180168A (en) | A kind of preparation method of high-strength ceramic biology 3D printing material | |
| CN109181236A (en) | A kind of preparation method of quick-setting biological 3D printing material | |
| CN105749341B (en) | A kind of three-dimensional rack and preparation method thereof of chitosan nano micrometer fibers structure | |
| CN109762284A (en) | A kind of preparation method of the low temperature resistant composite biological material of high temperature resistant | |
| CN108752605A (en) | A kind of preparation method of the high slow release hydrogel of high intensity | |
| Rajan et al. | Development of microwave absorbers from biopolymer composites | |
| Shyeed et al. | Additive Manufacturing of Hydrogels in Tissue Engineering |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20190122 |