CN109248342A - A kind of porous bioglass bone renovating material and preparation method thereof, purposes - Google Patents
A kind of porous bioglass bone renovating material and preparation method thereof, purposes Download PDFInfo
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Abstract
The invention belongs to glass Bone Defect Repari technical field, a kind of porous bioglass bone renovating material and preparation method thereof, purposes, after porous bioglass bone renovating material obtained is implanted organism, good biocompatibility are disclosed;The bone renovating material of preparation is made of the inorganic material of natural macromolecular material and class bone component, its surface can form good interfacing relationship with cell, the position of any bone defect can be implanted into bone surgery, it is unrestricted using position, there is good biocompatibility, no cytotoxicity, without immunological rejection.Using bone renovating material prepared by method of the invention, there is good bone-inducting active, it can very fast bone induction and regeneration reconstruction;Using bone renovating material prepared by method of the invention, there is good degradation property, material is gradually degraded with the growth of area of new bone, and occupy-place is not present, does not interfere the reconstruction of bone;Shorten repairing efficiency.
Description
Technical field
The invention belongs to glass Bone Defect Repari technical field more particularly to a kind of porous bioglass bone renovating material and
Preparation method, purposes.
Background technique
Glass bone osteogenesis imperfecta congenita, lead by collagenous fibres lesion caused by being a kind of defect because of inborn genetic
Sclerotin crisp fritter is caused, is unable to undergo to collide glass-like, patient sneezes, turns over the possible fracture of a body, therefore can not normally transport
It is dynamic, whole-body muscle most likely atrophy.Clinical symptoms is that osteoporosis is fragile, is easily fractured.However, the glass bone of existing preparation
Repair materials poor biocompatibility;Meanwhile it is relatively low to the efficiency of Bone Defect Repari, need long time could be with bone densification
In conjunction with.
In conclusion problem of the existing technology is:
The glass bone renovating material poor biocompatibility of existing preparation;
Meanwhile it is relatively low to the efficiency of Bone Defect Repari, need long time that could combine with bone densification.
In the prior art, it is theoretically unsound for the application of porous bioglass bone renovating material.
Summary of the invention
In view of the problems of the existing technology, the present invention provides a kind of porous bioglass bone renovating material and its
Preparation method, purposes.
The invention is realized in this way a kind of porous bioglass bone renovating material component includes:
12 parts of calcium chloride, 8 parts of barium chloride, 12 parts of dehydrated alcohol, 6 parts of phosphorus pentoxide, 10 parts of ethylenediamine tetra-acetic acid, metatitanic acid
Four 30 parts of butyl ester lecithin, 25 parts of animal bone morphogenetic protein, 10 parts of chitosan, 5 parts of hydroxyapatite, gelatin part 10, ammonia
13 parts of water, 20 parts of deionized water.
Another object of the present invention is to provide a kind of preparation methods of porous bioglass bone renovating material, including
Following steps:
Step 1 takes lecithin and animal bone morphogenetic protein, is ground to uniformly mixed;Add into obtained mixture
Enter chitosan and hydroxyapatite, is ground to evenly dispersed;
Upper step mixture is added gelatin, calcium chloride, barium chloride, deionized water and mixed, then heated, prepare by step 2
At mixed solution;
Step 3 is fitted into sonic oscillation instrument after mixing mixed solution and ethylenediamine tetra-acetic acid, and sonic oscillation reaction obtains
It is spare to self-control complex liquid;
Step 4 is added in reaction kettle, first with the revolving speed of 220r/min after mixing dehydrated alcohol and phosphorus pentoxide
It is stirred 25min and obtains mixed liquor, then adjust pH of mixed to 9.3 for 20% ammonium hydroxide with mass fraction, obtain precursor liquid;
Step 5 instills spare self-control complex liquid in above-mentioned precursor liquid, and microwave irradiation processing, after treatment is stood
Ageing, is separated by filtration to obtain filter residue after ageing;
Butyl titanate and dehydrated alcohol are mixed, are stirred 35min with glass bar, obtain four fourth of metatitanic acid by step 6
Ester ethanol solution, then will above-mentioned filter residue and butyl titanate ethanol solution obtained mix after be fitted into conical flask, then by taper
Bottle is placed on shaking table, shaking table oscillating reactions 5h, closed conical flask and stands 28h after oscillating reactions;
Reactant after above-mentioned standing is poured into Buchner funnel, is filtered respectively with dehydrated alcohol and deionized water by step 7
13min is washed, the washing filter residue on Buchner funnel filter paper is obtained, washing filter residue is first put into oven drying, then wash dry
Filter residue moves into tube type resistance furnace, with the heating rate temperature programming of 10 DEG C/min to 1200 DEG C, discharges after high-temperature calcination 3h, i.e.,
Obtain porous bioglass bone renovating material.
Further, the hydroxyapatite is as follows using chemical blowing process synthetic method:
Firstly, the nanometer hydroxyapatite and polyamide that ratio is 2:5 are mixed into composite powder;
Then, it is added the dehydrated alcohol of a certain amount of foaming agent, the ratio of composite powder and foaming agent is 50:1, is sufficiently stirred
It is uniformly mixed it, is put into 80 DEG C of vacuum drying oven and dries, dehydrated alcohol is allowed sufficiently to volatilize;
Finally, the drying sub-material of different quality is taken to be respectively put into 50mm × 30mm × 10mm grinding tool, it is put into plate sulphur
Change machine and the pressure for keeping 4MPa, constant temperature 1h under the conditions of 300 DEG C, are cooled to room temperature.
Further, the calcium chloride and barium chloride and the mass ratio of deionized water are 3:1:20, mixed solution and second two
The mass ratio 15:1 of amine tetraacethyl, the frequency of sonic oscillation reaction are 25~30kHz, and the time of sonic oscillation reaction is 1~2h.
Further, the mass ratio of the self-control complex liquid and precursor liquid is 1:3, and the temperature of microwave irradiation processing is 65 DEG C,
The power of microwave irradiation processing is 350W, and the time of microwave irradiation processing is 13h, and still aging temperature is 5 DEG C, still aging
Time be 25h.
Another object of the present invention is to provide porous bioglass bone renovating material performances described in a kind of verifying
Animal model constructing method, the animal model constructing method includes:
Step 1: taking different types of mouse and rat several are cultivated, weight 17g~110g, male and female dual-purpose;
Step 2: aseptically being extracted glass bone liquid, be diluted by a certain percentage with physiological saline, and experiment is taken to use
Mouse or rat press sterile working in its hind leg bone and inoculate mouse or rat glass bone sterile diluent, weigh after 24 hours
Grouping;
Step 3: being grouped into glass bone control group at random, the large, medium and small dosage group of test medicine, and every group 10, wherein glass
Animal 20 in glass bone control group, the daily 20mg/kgi.p of CTX, remaining each group inoculate daily;
Step 4: model control group gives isometric(al) aquae destillata, separately sets intact animal and is not inoculated with glass bone animal, inoculation etc.
Physiological saline is measured as false inoculation group 10, gavages isometric(al) aquae destillata daily as Normal group, once a day, continuous 30
It.
Further, it is discontinued next day, weight of weighing takes hind leg bone to detect, and t is examined between as a result organizing, and is carried out different groups and is compared.
Further, the method for taking hind leg bone to detect is carried out by image detection equipment, comprising:
Step 1, the transmission modeling of photon weak scattering hind leg bone;
Acquired projections data when step 2, light source and CCD camera staggered relatively;
Step 3 rebuilds hind leg bone attenuation coefficient;
Acquired projections data when step 4, light source and CCD camera are angularly offset placement;
Step 5 rebuilds hind leg bone scattering coefficient;
Step 6 calculates the absorption coefficient of hind leg bone.
Advantages of the present invention and good effect are as follows:
After porous bioglass bone renovating material produced by the present invention is implanted organism, good biocompatibility, and
And in organism motion process, bone renovating material can play piezoelectric effect to generating faint charge, these faint charges
The formation that can promote osteocyte body has more efficient repairing effect compared to simple calcium hydroxy phosphate bone renovating material,
It has a extensive future.Meanwhile bone renovating material prepared by the present invention is by the inorganic material of natural macromolecular material and class bone component
Composition, surface can form good interfacing relationship with cell, the position of any bone defect can be implanted into bone surgery, is made
It is unrestricted with position, there is good biocompatibility, no cytotoxicity, without immunological rejection;Using method of the invention
Prepared bone renovating material has good bone-inducting active, can very fast bone induction and regeneration reconstruction;Using method of the invention
Prepared bone renovating material has good degradation property, and material is gradually degraded with the growth of area of new bone, and there is no account for
Position, does not interfere the reconstruction of bone;Using bone renovating material prepared by method of the invention, with high porosity be connected
Pore morphology, and higher specific surface area is formed, space advantageous enough is provided for the growth of cell;Shorten repairing efficiency.
Animal model constructing method provided by the invention: taking different types of mouse and rat several are cultivated, body
Weight 17g~110g, male and female dual-purpose;Glass bone liquid is aseptically extracted, is diluted, is taken by a certain percentage with physiological saline
Laboratory mice or rat inoculate mouse or rat glass bone sterile diluent by sterile working in its hind leg bone, and 24 hours
Weighing grouping afterwards;It is grouped into glass bone control group at random, the large, medium and small dosage group of test medicine, every group 10, wherein glass bone
Animal 20 in control group, the daily 20mg/kgi.p of CTX, remaining each group inoculate daily;Model control group is steamed to isometric(al)
Water is slipped, intact animal is separately set and is not inoculated with glass bone animal, inoculation same amount of normal saline gavages daily as false inoculation group 10
Isometric(al) aquae destillata is as Normal group, once a day, continuous 30 days.It being discontinued next day, weight of weighing takes hind leg bone to detect,
As a result t is examined between organizing, and is carried out different groups and is compared.
The method for taking hind leg bone to detect is carried out by image detection equipment, comprising: the transmission modeling of photon weak scattering hind leg bone;
Acquired projections data when light source and CCD camera staggered relatively;Rebuild hind leg bone attenuation coefficient;Light source and CCD camera offset are certain
Acquired projections data when angle is placed;Rebuild hind leg bone scattering coefficient;Calculate the absorption coefficient of hind leg bone.
Porous bioglass bone renovating material performance detection obtained can be achieved, provide foundation for its purposes.
Detailed description of the invention
Fig. 1 is that the present invention implements porous bioglass bone renovating material provided and preparation method thereof, purposes process
Figure.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
Application principle of the invention is further described with reference to the accompanying drawing.
Porous bioglass bone renovating material component provided in an embodiment of the present invention includes:
12 parts of calcium chloride, 8 parts of barium chloride, 12 parts of dehydrated alcohol, 6 parts of phosphorus pentoxide, 10 parts of ethylenediamine tetra-acetic acid, metatitanic acid
Four 30 parts of butyl ester lecithin, 25 parts of animal bone morphogenetic protein, 10 parts of chitosan, 5 parts of hydroxyapatite, gelatin part 10, ammonia
13 parts of water, 20 parts of deionized water.
As shown in Figure 1, the preparation method of porous bioglass bone renovating material provided in an embodiment of the present invention, including
Following steps:
S101 takes lecithin and animal bone morphogenetic protein, is ground to uniformly mixed;It is added into obtained mixture
Chitosan and hydroxyapatite are ground to evenly dispersed;
Upper step mixture is added gelatin, calcium chloride, barium chloride, deionized water and mixed, then heated, be configured to by S102
Mixed solution;
S103 is fitted into sonic oscillation instrument after mixing mixed solution and ethylenediamine tetra-acetic acid, and sonic oscillation reaction obtains
Make complex liquid by oneself, it is spare;
S104 is added in reaction kettle after mixing dehydrated alcohol and phosphorus pentoxide, is first stirred with the revolving speed of 220r/min
It mixes mixing 25min and obtains mixed liquor, then adjust pH of mixed to 9.3 for 20% ammonium hydroxide with mass fraction, obtain precursor liquid;
S105 instills spare self-control complex liquid in above-mentioned precursor liquid, and microwave irradiation processing, after treatment stands old
Change, is separated by filtration to obtain filter residue after ageing;
Butyl titanate and dehydrated alcohol are mixed, are stirred 35min with glass bar, obtain butyl titanate by S106
Ethanol solution, then will above-mentioned filter residue and butyl titanate ethanol solution obtained mix after be fitted into conical flask, then by conical flask
It is placed on shaking table, shaking table oscillating reactions 5h, closed conical flask and stands 28h after oscillating reactions;
Reactant after above-mentioned standing is poured into Buchner funnel by S107, is filtered and is washed respectively with dehydrated alcohol and deionized water
13min is washed, the washing filter residue on Buchner funnel filter paper is obtained, washing filter residue is first put into oven drying, then dry washing is filtered
Slag moves into tube type resistance furnace, with the heating rate temperature programming of 10 DEG C/min to 1200 DEG C, after high-temperature calcination 3h discharging to get
Porous bioglass bone renovating material.
Hydroxyapatite provided by the invention is as follows using chemical blowing process synthetic method:
Firstly, the nanometer hydroxyapatite and polyamide that ratio is 2:5 are mixed into composite powder;
Then, it is added the dehydrated alcohol of a certain amount of foaming agent, the ratio of composite powder and foaming agent is 50:1, is sufficiently stirred
It is uniformly mixed it, is put into 80 DEG C of vacuum drying oven and dries, dehydrated alcohol is allowed sufficiently to volatilize;
Finally, the drying sub-material of different quality is taken to be respectively put into 50mm × 30mm × 10mm grinding tool, it is put into plate sulphur
Change machine and the pressure for keeping 4MPa, constant temperature 1h under the conditions of 300 DEG C, are cooled to room temperature.
Calcium chloride and barium chloride provided by the invention and the mass ratio of deionized water are 3:1:20, mixed solution and second two
The mass ratio 15:1 of amine tetraacethyl, the frequency of sonic oscillation reaction are 25~30kHz, and the time of sonic oscillation reaction is 1~2h.
The mass ratio of self-control complex liquid and precursor liquid provided by the invention is 1:3, and the temperature of microwave irradiation processing is 65 DEG C,
The power of microwave irradiation processing is 350W, and the time of microwave irradiation processing is 13h, and still aging temperature is 5 DEG C, still aging
Time be 25h.
Animal model constructing method provided in an embodiment of the present invention includes:
Step 1: taking different types of mouse and rat several are cultivated, weight 17g~110g, male and female dual-purpose;
Step 2: aseptically being extracted glass bone liquid, be diluted by a certain percentage with physiological saline, and experiment is taken to use
Mouse or rat press sterile working in its hind leg bone and inoculate mouse or rat glass bone sterile diluent, weigh after 24 hours
Grouping;
Step 3: being grouped into glass bone control group at random, the large, medium and small dosage group of test medicine, and every group 10, wherein glass
Animal 20 in glass bone control group, the daily 20mg/kgi.p of CTX, remaining each group inoculate daily;
Step 4: model control group gives isometric(al) aquae destillata, separately sets intact animal and is not inoculated with glass bone animal, inoculation etc.
Physiological saline is measured as false inoculation group 10, gavages isometric(al) aquae destillata daily as Normal group, once a day, continuous 30
It.
It is discontinued next day, weight of weighing takes hind leg bone to detect, and t is examined between as a result organizing, and is carried out different groups and is compared.
The method for taking hind leg bone to detect is carried out by image detection equipment, comprising:
Step 1, the transmission modeling of photon weak scattering hind leg bone;
Acquired projections data when step 2, light source and CCD camera staggered relatively;
Step 3 rebuilds hind leg bone attenuation coefficient;
Acquired projections data when step 4, light source and CCD camera are angularly offset placement;
Step 5 rebuilds hind leg bone scattering coefficient;
Step 6 calculates the absorption coefficient of hind leg bone.
The photon weak scattering hind leg bone transmission, which models, includes:
Indicate the upper incident photon of Γ-to positionAnd direction isWhen the distance propagated, then:
WhereinForThe radiancy at place indicates in unit solid angle, the unit time is interior, by perpendicular to unit side
To vectorUnit area on mean power flux density, dimension is W/ (m2.Sr);K0For the photon ballistic propagation of introducing
Operator,Indicate that hind leg bone existsTotal attenuation coefficient at place,Indicate the absorption system of hind leg bone
Number,Indicate scattering coefficient;
It re-defines:
Wherein K is the photon equilibrium state propagation operator introduced,For normalized scattering phase function, indicate photon from
DirectionIt scatters toThe probability in direction meetsD Ω ' expression unit direction vectorCorresponding solid angle
Infinitesimal;Define m0=K0gin,Then there is nn+1=Kmn(n >=0), thusTotal radiance at place are as follows:
Wherein mnIt indicates to scatter through n times and reachRadiancy component;When photon diffusion zone propagate when, the spectral radius of K
ρ (K) value is close to 1, when photon is in weak scattering regional spread, ρ (K) > > 1, in this case, as n → ∞,
Fast convergence;
Then, in output boundary Γ+The total amount of data g that upper description detector receivesout, i.e.,
To:
Wherein A is the matrix for describing photon transmission, A0、A1And A2Ballistic transport is described respectively, primary scattering transmits and multiple
Hop is scattered, g is defined0=A0gin, g1=A1ginRespectively indicate the ballistic transport component and primary scattering point in measurement numerical value
Amount, then know:
Incident light direction isIts direction is after primary scatteringThen in above formula aboutIntegral only one
There is value on special angle, takesThe value of coefficient k is by phase functionIt determines, together
Shi Dingyi Respectively indicate light
Attenuation after son scatters and before scattering, then have:
The acquisition data for projection includes:
Firstly, light source and detector is staggered relatively, then light source is irradiated hind leg bone with directional light and telecentricity is saturating
The CCD camera of mirror coupling receives parallel incident light;
Secondly, irradiation source carries out floor projection tomoscan to the hind leg bone being fixed in electronically controlled rotary table, irradiation source is adopted
With laser, and light is expanded using telecentric lens and irradiates hind leg bone for directional light;
Finally, using the data for projection of detector acquisition hind leg bone, one angle of the every rotation of the method hind leg bone of acquisition is adopted
Collect a data for projection, in a computer by the data for projection acquired every time record.
Attenuation coefficient is rebuild in the calculating
Using the directional light of space uniform distribution to hind leg bone g in OPT imaginginIt is irradiated, is acquired by CCD camera
The irradiation light that no hind leg bone blocks measures incident intensity;It is rightIt is left
Right both sides are the same as divided by ginAnd take negative logarithm, then:
Collect 360 degree of measurement data G0Afterwards, inverse Radon is realized using accurate efficient filter back-projection reconstruction algorithm
Transformation calculates attenuation coefficient, i.e. μt=FBP (G0)。
The acquired projections data that are angularly offset include:
The sensitive detection parts of OPT system are angularly offset θ to place, irradiation source is to the hind leg being fixed in electronically controlled rotary table
Bone carries out floor projection tomoscan, and irradiation source uses laser, and is expanded light for directional light irradiation using telecentric lens
Hind leg bone;
Using detector acquisition hind leg bone data for projection, every rotation one angle acquisition, one data for projection of hind leg bone,
In a computer by the data for projection acquired every time record.
The reconstruction scattering coefficient is by formulag1Contain OPT
The influence scattered in imaging, as the angle acquisition data g from a certain determination1When,WithScattering angle determine, coefficient k one
The constant of a determination;Both sides are the same as divided by kgin, then have:
Known by above formulaProlong for scattering coefficientThe weighting Radon in direction is converted, institute weighted value ω1(t) and ω2(t)
It is function related with attenuation coefficient, it willDiscretization is simultaneously expressed as follows with a matrix type:
Wμs=G1;
Wherein W indicates the weight matrix after discretization, μsAnd G1Scattering coefficient vector sum different angle is respectively indicated to measure
The AVHRR NDVI vector arrived establishes following objective function using the weighted least-squares criterion with penalty function:
Wherein the first item of expression formula is the approximate expression form of likelihood function, Section 2 R (μs) it is regular terms, usual root
It is constructed according to the prior information of image, β is regularization factors, and Matrix C is covariance matrix;With niIndicate ccd detector inspection
The scattered photon number measured, corresponding covariance matrix indicate are as follows:
Using optimal method to Φ (μs) objective function solve, that is, find out scattering coefficient:
μs=argmin Φ (μs)。
The calculated result for calculating absorption coefficient and utilizing step 3 and step 5, calculates the absorption coefficient of hind leg bone, benefit
With relational expression μt=μa+μsCalculate the absorption coefficient μ of hind leg bonea
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Made any modifications, equivalent replacements, and improvements etc., should all be included in the protection scope of the present invention within mind and principle.
Claims (8)
1. a kind of porous bioglass bone renovating material, which is characterized in that the porous bioglass Bone Defect Repari material
Expect component by mass parts by:
12 parts of calcium chloride, 8 parts of barium chloride, 12 parts of dehydrated alcohol, 6 parts of phosphorus pentoxide, 10 parts of ethylenediamine tetra-acetic acid, four fourth of metatitanic acid
30 parts of ester lecithin, 25 parts of animal bone morphogenetic protein, 10 parts of chitosan, 5 parts of hydroxyapatite, gelatin part 10, ammonium hydroxide 13
Part and 20 parts of deionized water compositions.
2. a kind of preparation method of porous bioglass bone renovating material as described in claim 1, which is characterized in that described
The preparation method of porous bioglass bone renovating material the following steps are included:
Step 1 takes lecithin and animal bone morphogenetic protein, is ground to uniformly mixed;Shell is added into obtained mixture
Glycan and hydroxyapatite are ground to evenly dispersed;
Upper step mixture is added gelatin, calcium chloride, barium chloride, deionized water and mixed, then heated, be configured to mix by step 2
Close solution;
Step 3 is fitted into sonic oscillation instrument after mixing mixed solution and ethylenediamine tetra-acetic acid, and sonic oscillation reaction obtains certainly
Complex liquid processed, it is spare;
Step 4 is added in reaction kettle after mixing dehydrated alcohol and phosphorus pentoxide, is first stirred with the revolving speed of 220r/min
Mixing 25min obtains mixed liquor, then adjusts pH of mixed to 9.3 for 20% ammonium hydroxide with mass fraction, obtains precursor liquid;
Step 5 instills spare self-control complex liquid in above-mentioned precursor liquid, and microwave irradiation processing, after treatment stands old
Change, is separated by filtration to obtain filter residue after ageing;
Butyl titanate and dehydrated alcohol are mixed, are stirred 35min with glass bar, obtain butyl titanate second by step 6
Alcoholic solution, then be fitted into conical flask after above-mentioned filter residue and butyl titanate ethanol solution obtained are mixed, then conical flask is put
On shaking table, shaking table oscillating reactions 5h closed conical flask and stands 28h after oscillating reactions;
Reactant after above-mentioned standing is poured into Buchner funnel by step 7, distinguishes filtering and washing with dehydrated alcohol and deionized water
13min obtains the washing filter residue on Buchner funnel filter paper, washing filter residue is first put into oven drying, then wash filter residue for dry
It moves into tube type resistance furnace, with the heating rate temperature programming of 10 DEG C/min to 1200 DEG C, discharging is after high-temperature calcination 3h to get more
Hole bioactive glass bone renovating material.
3. the preparation method of porous bioglass bone renovating material as claimed in claim 2, which is characterized in that the hydroxyl
Base apatite is as follows using chemical blowing process synthetic method:
Firstly, the nanometer hydroxyapatite and polyamide that ratio is 2:5 are mixed into composite powder;
Then, it is added the dehydrated alcohol of a certain amount of foaming agent, the ratio of composite powder and foaming agent is 50:1, and being sufficiently stirred makes it
It is uniformly mixed, is put into 80 DEG C of vacuum drying oven and dries, dehydrated alcohol is allowed sufficiently to volatilize;
Finally, the drying sub-material of different quality is taken to be respectively put into 50mm × 30mm × 10mm grinding tool, it is put into vulcanizing press
And the pressure of 4MPa is kept, constant temperature 1h under the conditions of 300 DEG C is cooled to room temperature.
4. the preparation method of porous bioglass bone renovating material as claimed in claim 2, which is characterized in that the chlorine
Change calcium and barium chloride and the mass ratio of deionized water be 3:1:20, the mass ratio 15:1 of mixed solution and ethylenediamine tetra-acetic acid,
The frequency of sonic oscillation reaction is 25~30kHz, and the time of sonic oscillation reaction is 1~2h.
5. the preparation method of porous bioglass bone renovating material as claimed in claim 2, which is characterized in that it is described from
The mass ratio of complex liquid and precursor liquid processed is 1:3, and the temperature of microwave irradiation processing is 65 DEG C, and the power of microwave irradiation processing is
350W, the time of microwave irradiation processing are 13h, and still aging temperature is 5 DEG C, and the still aging time is 25h.
6. a kind of animal model building side for verifying porous bioglass bone renovating material performance as claimed in claim 2
Method, which is characterized in that the animal model constructing method includes:
Step 1: taking different types of mouse and rat several are cultivated, weight 17g~110g, male and female dual-purpose;
Step 2: glass bone liquid is aseptically extracted, is diluted by a certain percentage with physiological saline, takes laboratory mice
Or rat inoculates mouse or rat glass bone sterile diluent by sterile working in its hind leg bone, weighing point after 24 hours
Group;
Step 3: being grouped into glass bone control group at random, the large, medium and small dosage group of test medicine, and every group 10, wherein glass bone
Animal 20 in control group, the daily 20mg/kgi.p of CTX, remaining each group inoculate daily;
Step 4: model control group gives isometric(al) aquae destillata, separately sets intact animal and is not inoculated with glass bone animal, inoculation equivalent is raw
Salt water is managed as false inoculation group 10, gavages isometric(al) aquae destillata daily as Normal group, once a day, continuous 30 days.
7. animal model constructing method as claimed in claim 6, which is characterized in that be discontinued next day, weight of weighing takes hind leg bone
Detection, t is examined between as a result organizing, and is carried out different groups and is compared.
8. animal model constructing method as claimed in claim 7, which is characterized in that the method for taking hind leg bone to detect passes through image
Detection device carries out, comprising:
Step 1, the transmission modeling of photon weak scattering hind leg bone;
Acquired projections data when step 2, light source and CCD camera staggered relatively;
Step 3 rebuilds hind leg bone attenuation coefficient;
Acquired projections data when step 4, light source and CCD camera are angularly offset placement;
Step 5 rebuilds hind leg bone scattering coefficient;
Step 6 calculates the absorption coefficient of hind leg bone.
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