CN109240064A - Portable digital holographic microscope system based on AM335X processor - Google Patents
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- 238000005457 optimization Methods 0.000 claims abstract description 10
- 238000001093 holography Methods 0.000 claims description 14
- 238000009647 digital holographic microscopy Methods 0.000 claims description 10
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Classifications
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- G—PHYSICS
- G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
- G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
- G03H1/00—Holographic processes or apparatus using light, infrared or ultraviolet waves for obtaining holograms or for obtaining an image from them; Details peculiar thereto
- G03H1/0005—Adaptation of holography to specific applications
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/361—Optical details, e.g. image relay to the camera or image sensor
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/365—Control or image processing arrangements for digital or video microscopes
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- G—PHYSICS
- G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
- G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
- G03H1/00—Holographic processes or apparatus using light, infrared or ultraviolet waves for obtaining holograms or for obtaining an image from them; Details peculiar thereto
- G03H1/04—Processes or apparatus for producing holograms
- G03H1/0443—Digital holography, i.e. recording holograms with digital recording means
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- G—PHYSICS
- G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
- G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
- G03H1/00—Holographic processes or apparatus using light, infrared or ultraviolet waves for obtaining holograms or for obtaining an image from them; Details peculiar thereto
- G03H1/0005—Adaptation of holography to specific applications
- G03H2001/005—Adaptation of holography to specific applications in microscopy, e.g. digital holographic microscope [DHM]
Abstract
A kind of portable digital holographic microscope system based on AM335X processor, including optical path, power module and processing main plate, the processing main plate is connect with power module, the light path part uses the higher coaxial optical path of portability, collocation partially coherent LED light source and 100um micropore realize coherent light, the hologram obtained simultaneously using optical sensor record, is given data to ARM embedded platform by USB high-speed serial bus and carries out reconstruction processing；ARM embedded platform is realized using the angular spectrum algorithm after the LINUX operating system and optimization carried and is quickly rebuild, and the display of micro-image is then carried out by LCD liquid crystal display, while carrying the interaction that capacitive touch screen is convenient for user.Apparatus of the present invention are simple, device is general, cheap, have good portability, are suitble to promote the use of in production and living, provide amenities for the people.
Description
Technical field
The invention belongs to Digital holographic microscopy fields, and in particular to use AM335X embedded processing platform, LED point light
Source and cmos image sensor, LCD liquid crystal display screen and touch screen carry the hologram image note that LINUX operating system is realized
Record and the mobile type digital holographic microscope system reproduced.
Background technique
Digital micro-holography is microtechnic novel at present, for traditional microtechnic, has and protects
The characteristic of object information is deposited, convenient for later processing, while its characteristic easy to operate is but also it gradually replaces tradition micro-
Technology.Digital micro-holography is then to utilize calculating using the hologram of optical sensor (CDD or CMOS) record object
Machine simulates optical path characteristic and carries out holographic reconstruction to hologram, to obtain the strength information and phase information of object script, in turn
Obtain the blur-free imaging of object.
The optical path of the Digital Holography of mainstream is divided into off-axis digital holography and two kinds of coaxial digital holography at present: off-axis number
Word holography is separated object wave with reference wave using spectroscope, is then done again before optical sensor (CCD or CMOS)
It relates to, to record its hologram by optical sensor (CCD or CMOS).Since the optical path of off-axis digital holography is more complicated, because
This is present in laboratory substantially, can not good promotion and application.Coaxial digital holography then eliminates spectroscope, so that object wave
It propagates along the direction of optical path with reference wave, is then recorded by optical sensor (CCD or CMOS).Off-axis gaussian beam is compared, coaxially
Digital hologram optical path is simple, therefore is more suitable for the popularization and use in production and living.
But at present due to the complexity of the complexity of holographic light path and holographic reconstruction algorithm, digital micro-holography is basic
Computer PC platform is operated in, cannot be carried and used well.
Summary of the invention
In order to overcome Contemporary Digital holographic microphotography technology optical path complicated, equipment is huge, is unfavorable for used in production and living
Disadvantage, the present invention propose a kind of digital holographic microscopic imaging system based on coaxial optical path and embedded platform, which can
Entirely autonomous operation, does not assist by external devices, easy to carry and popularization.
The technical solution adopted by the present invention to solve the technical problems is: a kind of portable number based on AM335X processor
Word holographic microscope system, including optical path, power module and processing main plate；The processing main plate is connected with power module, the light
Road part is the acquisition and recording core of whole system, and the light path part includes:
LED section coherent point light sources, for providing light source required for holographic microphotography；
The micropore of 100um improves for cooperating point light source to increase the coherence of light source and rebuilds effect；
Loading plane, for placing to microorganism, while also, available resolution plate replaces facilitating debugging optical path；
COMS imaging sensor, for recording by microorganism and the hologram formed with reference to the interference of light, then by picture number
It is handled according to processing main plate is transferred to；
The processing main plate is the processing core of whole system, and the processing main plate includes:
COMS camera module passes through USB serial communication bus and microcontroller for cooperating record hologram data
It is communicated, the hologram data of acquisition is transferred to microcontroller；
Microcontroller, the data for will acquire are handled using the angular spectrum algorithm after optimization, after being rebuild
Micrograph, being touched as a result, being driven simultaneously by I2C interface after then driving the display of LCD liquid crystal display to rebuild by LCD drive module
It touches screen to interact with user, continues relevant treatment acording to the requirement of user, realize the interaction of whole system and user.
Further, in the light path part, cooperated using partially coherent point light source and 100um micropore, use in-line holographic
Optical path realizes the micromation of entire acquisition optical path, user-friendly.
Further, in the relevant red point light source of the LED section, there is partial ocoherence, while cooperating 100um micro-
Hole enhances its coherence, reaches requirement of its hologram to coherent source, realizes and preferably rebuilds effect.
Further, in the loading plane, it is adjusted at a distance from cmos image sensor and its is adjustable
The left-right and front-back position of loading plane realizes the multiple micro- reconstruction for building distance and with bigger reconstruction visual field, meets user
Many-sided demand.
The processing main plate utilizes ARM embedded main board AM335X combination flush type LINUX operating system, realizes that processing is flat
Embeddedization of platform reaches the micromation of holographic microphotography reconstruction, realizes the portability development of digital holographic microscopic imaging technology.
The angular spectrum algorithm is completed using QT and OPENCV, cooperates the library FFTW, realizes the quick operation of angular spectrum algorithm, can
Embedded platform is run on, realizes the micromation of digital micro-holography.
The interactive mode of LCD liquid crystal display combination touch screen: it realizes and realizes user's interaction using LCD display cooperation touch screen
Micromation.
Hologram acquisition part acquires hologram data using the camera of 1.4um pixel dimension size, by USB high
Speed data bus is transferred to embedded processing platform AM335X and carries out hologram processing.The principle of the present invention description: partially coherent
The light that LED light source issues forms spherical surface light by the miniature pin hole of 100um, and then the irradiation of spherical surface light is located at the sample in loading plane
On product, the Object light wave for having object information is formed, while having the reference wave for being directed through loading plane, reference wave and Object light wave exist
Optical sensor (CMOS) goes out to interfere, and the hologram of formation is interfered to be recorded to form number by optical sensor (CMOS)
The hologram of information, then data are sent to ARM embedded processing platform, calculate in conjunction with the angular spectrum after LINUX operating system and optimization
Method carries out holographic microphotography reconstruction to hologram, the object real image after being rebuild, it is carried out in real time by LCD liquid crystal display screen
It has been shown that, facilitates the direct observation of user, while if user needs to adjust some parameters, the capacitance touch on LCD liquid crystal display
Screen interacts operation to relevant parameter, obtains oneself conceivable optimal reconstruction effect.Meanwhile if user wants to check sample not
The position of sample is adjusted in the case where with position, user, and adjustable spiral knob carries out the adjusting of left-right and front-back, carries out full side
The observation of position.Further, since this system use LINUX operating system, developer can self-developing corresponding program, treated
The concentration of sample, the ASSOCIATE STATISTICSs such as quantity operation, facilitates this equipment to help the demand of different user group.
The present invention proposes a kind of mobile type digital holographic microscope system based on embedded platform, and this system uses part phase
Dry light LED increases its coherence as light source, using micropore, and cmos sensor chip is as memorization COMS clip, ARM embedded platform
For AM335X processor as calculating center, optical path is simple, easy to carry, can be good at using and promoting in production and living.
The system thoroughly leaves the dependence to computer (PC), goes to complete using the computing platform and display platform of embedded platform oneself
Real mobile type digital holographic microscope system is realized in the calculating and display of Digital holographic microscopy.
It is compared with the prior art, the present invention has the following advantages:
Structure of the invention is light, realizes holographic microscopic imaging in lesser space using holographic microphotography technology, has good
Portability.Integrated design of the present invention eliminates dependence of the traditional technology to computer (PC), accomplishes real portability, shifting
Dynamic property.The present invention uses good display interaction platform, eliminates dependence of the traditional technology to peripheral hardware, the mankind is facilitated to use.This hair
The bright angular spectrum algorithm using after optimization, cooperation LINUX system realizes that quick holographic is rebuild, and traditional algorithm is embedded well
Change, realizes embeddedization of digital micro-holography.The present invention uses partially coherent LED light source, cooperates micropore, optical sensing
Device (CMOS) realizes that structure is simple, and component is universal, and cheap, holographic microphotography works well, and is conducive to Digital holographic microscopy
Popularization and application of the technology in production and living.
Detailed description of the invention
Fig. 1 is Digital holographic microscopy system light path figure.
Fig. 2 is Digital holographic microscopy system flow chart.
Fig. 3 is mobile type digital holographic microscope system overall structure diagram.
Fig. 4 is design of circuit system figure.
Specific embodiment
It is further described in detail with reference to the accompanying drawing.
Fig. 1 provides the Digital holographic microscopy index path of this system, and light path part mainly includes the following aspects: part phase
Dry light source led 1001,100um microwell plate 1002, transparent loading plane 1003 (being also possible to resolving power test target), optical sensor
(CMOS) module 1004.Partially coherent light source LED issued section coherent light, forms spherical wave after 100um micropore, and then one
It partially passes through sample and forms the Object light wave for carrying sample message, while another part is not passed through sample as reference wave, Object light wave
It is interfered on optical sensor (CMOS) with reference wave, optical sensor (CMOS) records its interference information (namely object
The hologram of body), by the conversion of optical sensor module, it is converted into being input to the tone by usb bus 4004 after digital signal
Connected ARM embedded platform mainboard 4000, in conjunction with LINUX system and optimization after angular spectrum algorithm to digital signal at
Reason, and then the strength information and phase information of sample are obtained, finally reconstructed results are shown by LCD signal bus 4009 again
On LCD4002 liquid crystal display, if desired obtain the quantity of sample, the information such as density, it is direct that developer can develop related application
To relevant information.
Total system using partially coherent LED light source, the miniature pin hole of 100um, loading plane, optical sensor (CMOS),
Embedded platform mainboard, LCD liquid crystal display and capacitive touch screen show a few part structures such as interaction platform and external protector
At.
(1) partially coherent LED light source: most of light source is partially coherent LED light source on the market, compared to laser light source
Have the characteristics that cheap, simultaneously because laser light source can generate a large amount of speckles, needs to carry out the processing of algorithm in system, it is right
For embedded platform, the system resource needed is excessive, therefore this system is using the micro- of partially coherent LED light source and 100um
Hole achievees the effect that relevant, not only save the cost but also had saved system resource, and had been suitable for embedded platform, and had had good portability.
(2) 100um micropore: since partially coherent light effect is undesirable, this system proposes the phase for increasing light source using micropore
Stemness compares laser, can be very good to eliminate speckle, saves system resource, while it is very big superfluous that the selection of light source is had
Yu Xing, suitable for the Digital holographic microscopy identification under various different situations.
(3) loading plane: loading plane is matched using transparent glass and helical rotation knob is realized, pullable, is being replaced
When micro- resource, loading plane is pulled out, is pushed into after then replacing, it is easy to operate, while can be turned by two spirals
Dynamic knob realizes that the left-right and front-back of loading plane is mobile, realizes various observations, rather than can only micro- fixation a part
(4) optical sensor (CMOS), this system optical sensor use 8,000,000 pixels, and the size of pixel is
1.4um can be realized the accurate reproduction of small items.
(5) embedded platform mainboard: this system uses ARM embeded processor AM335X platform motherboard, which has
Good portability and low power consumption characteristic cooperate LINUX embedded OS, can be realized entire digital micro-analysis imaging system
Acquisition hologram, reconstruction and reproducing processes, while the embedded platform mainboard cost is cheap, easy to carry is more suitable for producing
Popularization and use in life.
(6) show interaction platform: this system shows interaction platform using integrated LCD liquid crystal display and capacitive touch screen,
It does not need to can be realized as good interaction by extraneous any device, interaction logic is suitble to human manipulation, hand in convenience, simultaneously
Portability greatly increases, and is conducive to the popularization and use in production and living.
(7) external protector: external protector is constituted using 3D printing plastics, is mainly plastics using plastics protection
Lightweight, while can be good at the interference that Backup lightpath does not receive ambient light, reach good reconstruction effect, while in protecting
Portion's device does not receive damage, due to the lightweight of plastics, whole system is made to have better portability.
Fig. 2 be Digital holographic microscopy system flow chart, system operational process as shown, when system unlatching after, system root
Related initialization is carried out according to initial situation, mainly includes to prepare to rebuild calculative reconstruction two-dimensional array, so in initialization
Start the hologram information that acquisition is received by optical sensor (CMOS) afterwards, image information is embedded flat to ARM by USB transmission
Platform carries out holographic reconstruction processing, after processing according to require to see whether the statistical informations such as further progress concentration, quantity, at requirement
Reason, then continue to handle, and otherwise by the LCD micrograph that shows that treated, receives user later and interactively enters, to user's
It is required that being further processed, the micrograph after the operation real-time display reconstruction of front is repeated if no user input, last root
Judge whether system finishing according to conclusion button.
Fig. 3 mobile type digital holographic microscope system overall appearance structural representation, whole system is mainly by portions several in figure
It point constitutes, top is the in-line holographic light path part that LED point light source 3001 and 100um micropore 3002 form, and 3003 is carry in figure
Object piece, 3004 be imaging sensor, and the hologram of object mainly on acquisition micro slide, 3005 be embeded processor mainboard,
Cooperation 3006USB serial data bus obtains 3004 hologram datas transmitted, then carries out holographic reconstruction processing again.
3009 have collectively constituted display interactive system for touch screen and 3010LCD display screen, wherein 3007 be LCD bus, complete insertion
Display output on formula processor 3005 to LCD display 3010.3008 be I2C bus, complete embeded processor 3005 with
Data transmission between touch screen 3009.Whole device small volume, is convenient for carrying, and has good portability.
Fig. 4 is design of circuit system figure of the present invention, and 4003 be LED point light source corresponding in Fig. 1 1001 in figure, and cooperation is schemed
Micropore 1002 in one realizes the generation of coherent light.4001 be optical sensor (CMOS) mould corresponding in Fig. 1 1004 in figure
Group is connected by 4004USB high-speed serial bus in figure with ARM embedded processor platform, the hologram image number that will acquire
According to being transferred to ARM embedded processor platform, ARM embedded processor platform 4000 to hologram data by optimization after
Angular spectrum algorithm performs corresponding processing, and obtains micrograph, then gives LCD liquid crystal display by LCD parallel data bus line 4009
Screen 4002 is shown, while capacitive touch screen 4006 is interacted with user, obtains user's input, it is logical to also pass through I2C
Letter bus 4010 gives the processing of ARM embedded processor platform, realizes the real-time, interactive with user.User's debugging is provided simultaneously to connect
Mouth 4007 facilitates debugging efforts and the secondary development of developer.
For the problem that the optical path of current experiment room digital micro-holography is complicated, expensive: present invention proposition is adopted
With coaxial digital holography optical path, this optical path can be very good to solve the problems, such as that optical path is complicated, be difficult to build, be difficult to be miniaturized, together
When this system using partially coherent LED as light source, its coherence is increased using micropore, reaches required by holographic recording relevant
Property light, since partially coherent LED is cheap, can be very good control equipment cost, convenient for promote the use of.
Aiming at the problem that conventional digital holographic microphotography technology depends on computer (PC) platform: (1) present invention proposes to use
Angular spectrum algorithm after optimization is as holographic reconstruction algorithm.At present there are mainly three types of the holographic reconstruction algorithms of mainstream: Fresnel is rebuild
Algorithm, convolution algorithm for reconstructing, angular spectrum algorithm for reconstructing, since holographic reconstruction needs biggish calculation amount, these types is calculated at present
Method is present in computer (PC) platform substantially.Fresnel algorithm for reconstructing only needs a Fourier transformation, rebuilds fast speed, but calculate
Fresnel approximation is taken in method, therefore there is limitation, is not suitable for rebuilding apart from lesser situation.It is needed in convolution algorithm reconstruction process
Will Fourier transformation three times, reconstruction time is longer, and lateral resolution can decline with the increase for rebuilding distance.Angular spectrum algorithm
A Fourier transformation and an inverse Fourier transform are needed in reconstruction process, and it is moderate to rebuild speed.And the algorithm scope of application
Extensively, preferable effect can be played in the diffraction of various different distances.Therefore the angular spectrum algorithm after optimization is used in the present system
It is reconstructed.And it is optimized in terms of speed, Fourier transformation is carried out using the library FFTW, accelerates Fourier
The speed of transformation and inverse transformation.There is it also in embedded platform and rebuilds effect and reconstruction speed well.It is disobeyed to realize
Rely traditional calculations platform (PC), makes its portability.(2) it is directed to the not portability of computer (PC) platform, present invention proposition is based on
The Digital holographic microscopy software systems of LINUX system, the system are based on embedded processor platform, have USB serial data total
Line interface, LCD bus interface module, LCD liquid crystal display screen, I2C interface, LCD touch screen, UART communication connects, LED light source drives
Dynamic model block etc., system drive USB obtains the hologram data of CMOS, then complete to what is got using LINUX platform algorithm
Breath figure carries out micro- reconstruction, and reconstructed results are shown in LCD liquid crystal display screen, while it is further to cooperate touch screen to complete user
Operating interactive, realize that being completely embedded into the operation of formula realizes real portability without external device (ED).
The external display and aiming at the problem that input unit since conventional digital holographic microscope system: the present invention proposes one kind
The user interaction scheme inputted with touch screen is shown based on embedded LCD liquid crystal display, LCD liquid crystal display and capacitive touch screen are direct
It is connected on embedded platform, facilitates the operation of user, without external tasks device, realize entire digital micro-analysis imaging system
The portability of system.
The present invention realizes innovation in a plurality of directions, completely removes conventional digital holographic microphotography to external equipment (as calculated
Machine, keyboard, mouse etc.) dependence, fully achieve independence, while the device takes simple and practical optical path, the weight after optimization
Algorithm is built, so that it still has in LINUX embedded platform rebuilds effect well.Device is cheap simultaneously, whole system
It is easy to carry, convenient for Digital holographic microscopy system popularization with it is practical.
Claims (8)
1. a kind of portable dynamic formula Digital holographic microscopy system based on AM335X processor, it is characterised in that: including
Optical path (1000), power module (2000) and processing main plate (4000)；The processing main plate (4000) and power module
(2000) it connects, the optical path (1000) is partially the acquisition and recording core of whole system, and the optical path (1000) partially includes:
LED section coherent point light sources (1001), for providing light source required for holographic microphotography；
The micropore (1002) of 100um improves for cooperating point light source (1001) to increase the coherence of light source and rebuilds effect；
Loading plane (1003), for placing to microorganism, while also, available resolution plate replaces facilitating debugging optical path；
COMS imaging sensor (1004), for recording by microorganism and the hologram formed with reference to the interference of light, then by image
Data are transferred to processing main plate (4000) and are handled；
The processing main plate (4000) is the processing core of whole system, and the processing main plate (4000) includes:
COMS camera module (4001) passes through USB serial communication bus and microcontroller for cooperating record hologram data
Device (4011) is communicated, and the hologram data of acquisition is transferred to microcontroller (4011)；
Microcontroller (4011), the data for will acquire are handled using the angular spectrum algorithm after optimization, after obtaining reconstruction
Micrograph, then by LCD drive module (4009) driving LCD liquid crystal display (4002) display rebuild after as a result, leading to simultaneously
It crosses I2C interface (4010) driving touch screen (4006) to interact with user, continues relevant treatment acording to the requirement of user, realize
The interaction of whole system and user.
2. the portable digital holographic microscope system according to claim 1 based on AM335X processor, which is characterized in that
In optical path (1000) part, cooperated using partially coherent point light source (1001) and 100um micropore (1002), using coaxial complete
Optical path is ceased, realizes the micromation of entire acquisition optical path, it is user-friendly.
3. the portable digital holographic microscope system according to claim 1 or 2 based on AM335X processor, feature exist
In the LED section is concerned in red point light source (1001), has partial ocoherence, while 100um micropore (1002) being cooperated to increase
Its strong coherence, reaches requirement of its hologram to coherent source, realizes and preferably rebuilds effect.
4. the portable digital holographic microscope system according to claim 1 or 2 based on AM335X processor, feature exist
In it is adjusted at a distance from cmos image sensor (1004) and its adjustable load in the loading plane (1003)
The left-right and front-back position of object plane realizes the multiple micro- reconstruction for building distance and with bigger reconstruction visual field, meets user's
Many-sided demand.
5. the portable digital holographic microscope system according to claim 1 or 2 based on AM335X processor, feature exist
In the processing main plate (4000) combines flush type LINUX operating system using ARM embedded main board AM335X (4000), real
Embeddedization of existing processing platform, reaches the micromation of holographic microphotography reconstruction, realizes the portable of digital holographic microscopic imaging technology
Change development.
6. the portable word holographic microscope system according to claim 1 or 2 based on AM335X processor, which is characterized in that
The angular spectrum algorithm is completed using QT and OPENCV, cooperates the library FFTW, is realized the quick operation of angular spectrum algorithm, can be run on embedding
Enter formula platform, realizes the micromation of digital micro-holography.
7. the portable digital holographic microscope system according to claim 1 or 2 based on AM335X processor, feature exist
In LCD liquid crystal display (4002) combines the interactive mode of touch screen (4006): realizing and realized using LCD display cooperation touch screen
The micromation of user's interaction.
8. the portable digital holographic microscope system according to claim 1 based on AM335X processor, which is characterized in that complete
It ceases figure collecting part and hologram data is acquired using the camera of 1.4um pixel dimension size, passed by USB high speed data bus
It is defeated to carry out hologram processing to embedded processing platform AM335X.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110308547A (en) * | 2019-08-12 | 2019-10-08 | 青岛联合创智科技有限公司 | A kind of dense sample based on deep learning is without lens microscopic imaging device and method |
| CN112712877A (en) * | 2020-12-07 | 2021-04-27 | 西安电子科技大学 | Large-view-field high-flux high-resolution pathological section analyzer |
| CN114660916A (en) * | 2022-03-16 | 2022-06-24 | 李�杰 | Multi-angle holographic image display system and method |
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