CN109238998A - A kind of phenols is the method for substrate detection oil peroxidation value - Google Patents
A kind of phenols is the method for substrate detection oil peroxidation value Download PDFInfo
- Publication number
- CN109238998A CN109238998A CN201811201102.4A CN201811201102A CN109238998A CN 109238998 A CN109238998 A CN 109238998A CN 201811201102 A CN201811201102 A CN 201811201102A CN 109238998 A CN109238998 A CN 109238998A
- Authority
- CN
- China
- Prior art keywords
- phenols
- peroxide
- peroxidation value
- solution
- oil peroxidation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title claims abstract description 21
- 238000001514 detection method Methods 0.000 title claims abstract description 17
- 150000002989 phenols Chemical class 0.000 title claims abstract description 14
- 239000000758 substrate Substances 0.000 title claims abstract description 13
- 238000005502 peroxidation Methods 0.000 title claims abstract description 12
- GHMLBKRAJCXXBS-UHFFFAOYSA-N resorcinol Chemical compound OC1=CC=CC(O)=C1 GHMLBKRAJCXXBS-UHFFFAOYSA-N 0.000 claims abstract description 26
- 150000002978 peroxides Chemical class 0.000 claims abstract description 20
- 238000002835 absorbance Methods 0.000 claims abstract description 8
- 238000000870 ultraviolet spectroscopy Methods 0.000 claims abstract description 3
- 239000000243 solution Substances 0.000 claims description 27
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims description 10
- 229910021578 Iron(III) chloride Inorganic materials 0.000 claims description 5
- 238000006243 chemical reaction Methods 0.000 claims description 5
- 150000001875 compounds Chemical class 0.000 claims description 5
- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 claims description 5
- 239000002184 metal Substances 0.000 claims description 5
- 229910052751 metal Inorganic materials 0.000 claims description 5
- 239000012266 salt solution Substances 0.000 claims description 5
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 claims description 4
- 238000007872 degassing Methods 0.000 claims description 4
- 230000035484 reaction time Effects 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 2
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 claims description 2
- 239000001301 oxygen Substances 0.000 claims description 2
- 229910052760 oxygen Inorganic materials 0.000 claims description 2
- 235000013305 food Nutrition 0.000 abstract description 6
- 239000004519 grease Substances 0.000 abstract description 6
- 238000010561 standard procedure Methods 0.000 abstract description 3
- 239000003921 oil Substances 0.000 description 8
- 238000007254 oxidation reaction Methods 0.000 description 7
- 230000003647 oxidation Effects 0.000 description 5
- 238000002798 spectrophotometry method Methods 0.000 description 3
- 238000004448 titration Methods 0.000 description 3
- 238000010668 complexation reaction Methods 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 239000008157 edible vegetable oil Substances 0.000 description 2
- FBAFATDZDUQKNH-UHFFFAOYSA-M iron chloride Chemical compound [Cl-].[Fe] FBAFATDZDUQKNH-UHFFFAOYSA-M 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 229910021645 metal ion Inorganic materials 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000004566 IR spectroscopy Methods 0.000 description 1
- 238000011088 calibration curve Methods 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- YQHLDYVWEZKEOX-UHFFFAOYSA-N cumene hydroperoxide Chemical compound OOC(C)(C)C1=CC=CC=C1 YQHLDYVWEZKEOX-UHFFFAOYSA-N 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 235000019625 fat content Nutrition 0.000 description 1
- KTWOOEGAPBSYNW-UHFFFAOYSA-N ferrocene Chemical compound [Fe+2].C=1C=C[CH-]C=1.C=1C=C[CH-]C=1 KTWOOEGAPBSYNW-UHFFFAOYSA-N 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 235000013622 meat product Nutrition 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 230000033116 oxidation-reduction process Effects 0.000 description 1
- 238000006479 redox reaction Methods 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/33—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using ultraviolet light
Landscapes
- Physics & Mathematics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The invention belongs to field of detection of food safety, in particular to a kind of phenols is the method that substrate detects oil peroxidation value: the peroxide in grease aoxidizes substrate resorcinol, using ultraviolet-visible spectrophotometry, absorbance change is detected at 273nm, can calculate peroxide value.Method of the invention is quick on the draw, easy to operate, as a result accurately, quick and easy relative to National Standard Method (thiocyanate-ferric).
Description
Technical field
The invention belongs to field of detection of food safety, in particular to a kind of phenols is the side that substrate detects oil peroxidation value
Method.
Background technique
The oxidation of grease be food quality decline the reason of one of, especially to the fat contents such as edible oil, meat products compared with
High food.The peroxide generated after Oxidation of Fat and Oils is very unstable, is easily decomposed into many secondary oxidation products, can produce to human body
Raw certain harm, so the peroxide value (POV) of grease is the important quality indicator of the food such as edible oil.
The national standard method iodimetric titration of detection POV at present, but due to cumbersome, a large amount of organic solvents need to be protected from light and use,
Operating error is larger, is not suitable for real time monitoring, is especially not suitable for quickly detection.Spectrophotometry is quick, simple, easy
It operates, is at low cost, but detecting limit and precision and be no longer satisfied measurement requirement.Instrument analyzes (high performance liquid chromatography, gas phase color
Spectrum, infrared spectroscopy etc.) although high sensitivity, cost is generally relatively high, and operates relatively cumbersome.
102706872 A of patent CN discloses a kind of method of oxidation-reduction method detection grease, in redox reaction,
It generates complex compound and uses colorimetric determination complex compound, detection accuracy shortcoming later.Disclosed in 105241832 A of patent CN
Oxidation and complexation reaction occurs for cumyl hydroperoxide in solution of ferrocene and oil, generates complex compound, and examine using spectrophotometry
The method for surveying peroxide value, the temperature of Oxidation and complexation reaction is 50 DEG C, reaction time 60min, detection method of the invention, reaction
Time is short, and condition is simple.
Summary of the invention
The present invention by conjunction with oxidation reaction to phenols of phenols and the complex reaction of metal ion and peroxide, than
General spectrophotometry is simple, at low cost, detects quick and precisely, and selectivity with higher and sensitivity, for detection oil
The peroxide value of rouge provides a kind of fast and reliable method.
A kind of phenols is the method for substrate detection oil peroxidation value, which comprises the following steps:
Step 1: a certain amount of resorcinol solution is added and reacting metal salt solution generates complex compound；
Step 2.: peroxide solutions are added and are reacted；
Step 3.: using the absorbance after ultraviolet-uisible spectrophotometer detection reaction.
A kind of method of detection oil peroxidation value of this patent, the phenol solution in the step are resorcinol solution,
Metal salt solution is ferric chloride solution.
Water used in method carries out 10~30min of ultrasonic degassing in advance, the oxygen in air is avoided to influence.
Peroxide solutions in the step 2 can be hydrogen peroxide or other peroxide solutions, be also possible to contain
The oil sample of peroxide.
It is shaken up after peroxide solutions are added in step 2, this is quick on the draw quickly, and temperature can be room temperature, reaction
Several minutes of time.
Absorbance is detected at 273nm using ultraviolet-visible spectrophotometry in the step 3.
Further, phenol solution selects resorcinol solution in the step 1, and metal salt solution selects ferric chloride solution,
The concentration of resorcinol solution is 0.001g/mL.0.100g resorcinol solid accurately is weighed, after adding appropriate degasification distilled water to dissolve
It is transferred to 100mL volumetric flask, constant volume, configuration obtains the resorcinol solution of 0.001g/mL.It is solid accurately to weigh 0.100g iron chloride
Body, is transferred to 100mL volumetric flask, constant volume after adding appropriate degasification distilled water to dissolve, configuration obtains the ferric chloride solution of 0.001g/mL.
By experiment, the concentration ratio of resorcinol solution and ferric chloride solution is iron chloride: when resorcinol=5:1, there is preferable effect
Fruit.Resorcinol concentration is 0.01g/mL, 0.005g/mL, 0.004g/mL, 0.003g/mL, 0.002g/mL, 0.001g/mL
When, absorbance A is detected at 273nm273There is good linear relationship with concentration, corresponding calibration curve equation is Y=15130X+
0.0291, R2=0.9995.After adding hydrogen peroxide, absorbance reduces regularity, according to changing value.Addition 0.0001,
After the hydrogen peroxide of 0.0002~0.0009g/mL, absorbance change value and concentration have good linear relationship, corresponding mark
Directrix curve equation is Y=9916.7X+0.1389, R2=0.9904.With the Hydrogen peroxide standard product of same concentration, with national standard side
Method detection, obtains corresponding peroxide value (meq/kg), with A of the invention273Linear relationship equation: Y=125.9X- can be established
12.55 R2=0.9973.
The invention has the advantages that
(1) when for grease extraction in food, not enough or in grease peroxide content is too low, the result of titration measuring
Solution has deviation, and the present invention can be improved the accuracy of measurement.
(2) present invention applies complex reaction and the peroxide pair of phenolic hydroxyl group and metal ion using phenols as substrate
The oxidation reaction of classification, with the titration of national standard or colorimetric method complex steps and impacted factor it is more compared with, it is low in cost,
Program is simple, convenient and efficient.
(3) detection method of the invention, the reaction time is short, and condition is simple, is suitable for quickly detection.
Detailed description of the invention
Fig. 1 is abosrption spectrogram of the resorcinol solution in 240~300nm of various concentration.
Claims (6)
1. a kind of phenols is the method that substrate detects oil peroxidation value, which comprises the following steps:
Step 1: a certain amount of resorcinol solution is added and reacting metal salt solution generates complex compound；
Step 2.: peroxide solutions are added and are reacted；
Step 3.: using the absorbance after ultraviolet-uisible spectrophotometer detection reaction.
2. a kind of phenols according to claim 1 is the method that substrate detects oil peroxidation value, which is characterized in that described
Phenol solution in step 1 is resorcinol solution, and metal salt solution is ferric chloride solution.
3. a kind of phenols according to claim 1 is the method that substrate detects oil peroxidation value, which is characterized in that method
Used in water, in advance carry out ultrasonic degassing 10-30min, avoid the oxygen in air from influencing.
4. a kind of phenols according to claim 1 is the method that substrate detects oil peroxidation value, which is characterized in that described
Peroxide solutions in step 2 can be hydrogen peroxide or other peroxide solutions, be also possible to the oil containing peroxide
Rouge sample.
5. a kind of phenols according to claim 1 is the method that substrate detects oil peroxidation value, which is characterized in that described
It shaking up, is quick on the draw quickly after peroxide solutions are added in step 2, temperature can be room temperature, and several minutes of the reaction time.
6. a kind of phenols according to claim 1 is the method that substrate detects oil peroxidation value, which is characterized in that described
Absorbance is detected at 273nm using ultraviolet-visible spectrophotometry in step 3.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811201102.4A CN109238998B (en) | 2018-10-16 | 2018-10-16 | Method for detecting peroxide value of grease by using phenols as substrates |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811201102.4A CN109238998B (en) | 2018-10-16 | 2018-10-16 | Method for detecting peroxide value of grease by using phenols as substrates |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109238998A true CN109238998A (en) | 2019-01-18 |
| CN109238998B CN109238998B (en) | 2021-09-03 |
Family
ID=65053597
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811201102.4A Active CN109238998B (en) | 2018-10-16 | 2018-10-16 | Method for detecting peroxide value of grease by using phenols as substrates |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109238998B (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MX163598B (en) * | 1987-12-22 | 1992-06-04 | Indspec Chemical Corp | OXIDATION AND SUBSEQUENT DECOMPOSITION OF DIHYDROPEROXIDE |
| CN101201316A (en) * | 2007-09-04 | 2008-06-18 | 广西师范大学 | Spectrophotometric method for detecting trace amount horseradish peroxidase |
| CN101490556A (en) * | 2006-07-11 | 2009-07-22 | 保罗·奈杰尔·布罗克韦尔 | Indicator system for determining analyte concentration |
| CN102128798A (en) * | 2010-12-10 | 2011-07-20 | 江南大学 | Determination of trace amount hydrogen peroxide based on aniline polymerization reaction |
| US20130008803A1 (en) * | 2011-01-03 | 2013-01-10 | The Regents Of The University Of Michigan | Methods and Systems for Measurement of Tear Glucose Levels |
| CN105241832B (en) * | 2015-10-12 | 2018-04-06 | 天津科技大学 | A kind of oil peroxidation value detection method |
-
2018
- 2018-10-16 CN CN201811201102.4A patent/CN109238998B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MX163598B (en) * | 1987-12-22 | 1992-06-04 | Indspec Chemical Corp | OXIDATION AND SUBSEQUENT DECOMPOSITION OF DIHYDROPEROXIDE |
| CN101490556A (en) * | 2006-07-11 | 2009-07-22 | 保罗·奈杰尔·布罗克韦尔 | Indicator system for determining analyte concentration |
| CN101201316A (en) * | 2007-09-04 | 2008-06-18 | 广西师范大学 | Spectrophotometric method for detecting trace amount horseradish peroxidase |
| CN102128798A (en) * | 2010-12-10 | 2011-07-20 | 江南大学 | Determination of trace amount hydrogen peroxide based on aniline polymerization reaction |
| US20130008803A1 (en) * | 2011-01-03 | 2013-01-10 | The Regents Of The University Of Michigan | Methods and Systems for Measurement of Tear Glucose Levels |
| CN105241832B (en) * | 2015-10-12 | 2018-04-06 | 天津科技大学 | A kind of oil peroxidation value detection method |
Non-Patent Citations (4)
| Title |
|---|
| YONG-GUANG BI 等: "Dairy farming simulated organic wastewater of degradation Experiment by hydrogen peroxide", 《ADVANCED MATERIALS RESEARCH》 * |
| 刘典梅 等: "钡A-偶氮氯膦Ⅲ络合物探针分光光度法测定蛋白质", 《分析化学》 * |
| 张艳敏 等: "《有机化学》", 31 August 2015, 中国轻工业出版社 * |
| 赵丹 等: "《钢铁表面镀镍技术》", 31 July 2017, 冶金工业出版社 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109238998B (en) | 2021-09-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Chen et al. | A highly sulfite-selective ratiometric fluorescent probe based on ESIPT | |
| Ryan et al. | The kinetics and mechanisms of the complex formation and antioxidant behaviour of the polyphenols EGCg and ECG with iron (III) | |
| Fábos et al. | Bio-oxygenates and the peroxide number: a safety issue alert | |
| Szydłowska-Czerniak et al. | A silver nanoparticle-based method for determination of antioxidant capacity of rapeseed and its products | |
| Mutzel et al. | An improved method for the quantification of SOA bound peroxides | |
| CN104819970B (en) | A kind of method that super molecular complex fluorescence probe determines carbendazim in water | |
| WO2008149247A3 (en) | Kinetic determination of peracid and/or peroxide concentrations | |
| CN103760160A (en) | Colorimetric detection method of hydrogen peroxide | |
| Zyuzin et al. | Kinetics of methoxy-NNO-Azoxymethane hydrolysis in strong acids | |
| CN109238998A (en) | A kind of phenols is the method for substrate detection oil peroxidation value | |
| CN110174397B (en) | Application of colorimetric probe | |
| Mitić et al. | Analytical application of food dye Sunset Yellow for the rapid kinetic determination of traces of copper (II) by spectrophotometry | |
| CN105241832B (en) | A kind of oil peroxidation value detection method | |
| Afkhami et al. | Kinetic-spectrophotometric determination of trace amounts of As (III) based on its inhibitory effect on the redox reaction between bromate and hydrochloric acid | |
| CN201955298U (en) | Pocket chlorine dioxide colorimeter | |
| Adu et al. | Spectrophotometric determination of boron in food products by ester borate distillation into curcumin | |
| Morosanova et al. | Silica-titania xerogels: Solid-phase spectrophotometric and field test determination of hydrogen peroxide in disinfectants | |
| Bruno et al. | Fast and simple polarographic method for the determination of free and total sulphur dioxide in wines and other common beverages | |
| Birgani et al. | Development of a Dispersive Liquid–Liquid Microextraction Method Combined with UV-Visible Spectrophotometry for Determination of Trace Aluminum (III) in Water, Wastewater, Food, Biological, and Pharmaceutical Samples | |
| Lu et al. | A simple and highly selective 1, 2, 4, 5-tetrazine-based colorimetric probe for HSO 3− ion recognition in food | |
| Shariati-Rad et al. | Response Surface Methodology in Spectrophotometric Determination of Formaldehyde Using Ch romotropic Acid | |
| Zheng et al. | Chromo-chemodosimetric detection for Fe 2+ by Fenton reagent-induced chromophore-decolorizing of halogenated phenolsulfonphthalein derivatives | |
| CN108037117B (en) | Method and kit for rapidly detecting industrial hydrogen peroxide residue in food | |
| RU2386130C2 (en) | Method for catalytic spectrophotometric determination of cyanide anion | |
| Lim et al. | Fluorometric analysis of peracetic acid by the oxidative hydroxylation of a phenylboronic acid containing dye |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |