CN109221093A - A kind of cornea tissue long-term preservation liquid and preparation method thereof - Google Patents
A kind of cornea tissue long-term preservation liquid and preparation method thereof Download PDFInfo
- Publication number
- CN109221093A CN109221093A CN201811440809.0A CN201811440809A CN109221093A CN 109221093 A CN109221093 A CN 109221093A CN 201811440809 A CN201811440809 A CN 201811440809A CN 109221093 A CN109221093 A CN 109221093A
- Authority
- CN
- China
- Prior art keywords
- term preservation
- long
- preservation liquid
- cornea
- sodium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of cornea tissue long-term preservation liquid and preparation method thereof, including following components and content: 15~25g/L of sodium chondroitin sulfate, 0.5~4g/L of Sodium Hyaluronate, 0.5~3g/L of Dextran 40,15~30g/L of glycerol, 0.001~0.3g/L of Sodium Pyruvate, 0.002~2g/L of vitamin C, 0.05~0.7g/L of gentamicin sulphate, 1.4~2.2g/L of sodium bicarbonate, 5.0~6.5g/L of 4- hydroxyethyl piperazineethanesulfonic acid, water for injection 1L.The present invention is obtained the preservation liquid that can be used for cornea tissue long-term preservation, is particularly conducive to maintain the original collagen fiber structure of corneal lens and the transparency for a long time using the compound system of sodium chondroitin sulfate collaboration Sodium Hyaluronate and dextran；After the long-term preservation liquid cornea preservation tissue, rewarming be can be used directly, easy to operate, be not required to rehydration.
Description
Technical field
The present invention relates to a kind of cornea tissue long-term preservation liquid and preparation method thereof.
Background technique
Keratonosus is China's second diseases causing blindness, the existing about 4,000,000 corneal blindness patient in China, and annual corneal blindness patient is new
Increase more than 15 ten thousand people, corneal transplantation is still the main means for restoring eyesight, saving eyeball, however China's corneal material is seriously deficient
It is weary, compared with huge demand, an utterly inadequate amount.
Femtosecond laser small notch corneal stroma lens removal surgery (Small Incision Lenticule Extraction,
SMILE it is) one of maximum development in cornea refractive surgery field in recent years, for the correction of myopia and astigmatism, has high
Safety, validity, stability and predictability.The tissue that this part is taken out in corneal stroma we be known as " cornea is saturating
Mirror ".SMILE cornea tissue lens source is very rich, and the domestic annual SMILE that carries out performs the operation about 1,000,000, however SMILE hand
The corneal lens taken out in art this " byproduct " is often abandoned, and is not effectively utilized.Corneal lens is expected available
In correction long sight and to treat keratoconus, corneal dystrophy, Perforation denaturation (immunity of periphery cornea, non-infectious
Disease), the disease of cornea such as corneal stroma is thinning, ulcer of the cornea, perforation of cornea, and construct tissue engineered biological angle using its
The carrier etc. of film.
Corneal storage medium currently on the market is appropriate only for penetrating keratoplasty or interior skin grafting dermepenthesis, the low temperature suitable for cornea
Mid-term preservation mainly saves the activity of endothelial cell, and complicated composition are expensive, but limits in corneal lens preservation
Extensive use.However corneal lens should more be concerned with collagen fiber structure and transparency protection.
Summary of the invention
Goal of the invention: in view of the above-mentioned problems, an object of the present invention is to provide a kind of cornea tissue long-term preservation liquid, by
It is suitable for long-term preservation corneal lens, and the second object of the present invention is to provide a kind of this preparation method for saving liquid.
Technical solution: a kind of cornea tissue long-term preservation liquid, including following components and content:
Further, the pH value of the long-term preservation liquid is 7.2~7.6, and appearance is colourless to yellowish clear liquid, and
No suspended substance, without precipitating.
Further, the bacterial endotoxin < 0.25EU/ml of the water for injection.
A kind of preparation method of above-mentioned cornea tissue long-term preservation liquid, comprising the following steps:
Step S10: it weighs: weighing each component respectively according to formula ratio；
Step S20: it prepares: including:
Step S201: being added partial syringe water in container, control 25~60 DEG C of water temperature, be then respectively adding chondroitin sulfate
Plain sodium, Sodium Hyaluronate, Dextran 40, Sodium Pyruvate, sodium bicarbonate, 4- hydroxyethyl piperazineethanesulfonic acid, then fill into injection
Water simultaneously stirs 30~120min；
Step S202: controlling 16~40 DEG C of temperature for the mixed liquor of step S201, vitamin C be added, and adds part note
It penetrates with water and stirs 5~30min；
Step S203: the mixed liquor of step S202 is controlled 16~40 DEG C of temperature, gentamicin sulphate is added, adds portion
Divide water for injection and stirs 5~30min；
Step S204: the mixed liquor of step S203 is controlled 16~40 DEG C of temperature, glycerol is added, adds the injection of surplus
With water constant volume and 10~30min is stirred, obtains intermediate product；
Step S30: intermediate product examine is surveyed: being sampled to intermediate product, is carried out pH detection and appearance detection, detection is needed as do not met
PH adjusting is carried out to intermediate product, does not meet still, scraps after adjusting；
Step S40: aseptic filtration: advanced by step S30 detection qualified intermediate product or qualified intermediate product after adjusting
Row pre-filtering, then be filtered by 0.2 μm of PES filter to get the cornea tissue long-term preservation liquid.
Further, in step S30, it is 7.2~7.6 that pH, which detects qualified index, appearance detect qualified index be it is colourless extremely
Yellowish clear liquid, and no suspended substance, without precipitating.
Further, in step S40, pH is carried out to intermediate product and is adjusted using hydrochloric acid or sodium hydroxide solution.
Further, the long-term preservation liquid using low borosilicate ampoule bottle or middle borosilicate ampoule bottle it is filling, and jumped a queue and
It is closed to roll lid.
The principle of the present invention is: using the compound system of sodium chondroitin sulfate collaboration Sodium Hyaluronate and dextran, most
The cornea preservation tissue of limits can especially maintain the original collagen fiber structure of corneal lens and the transparency for a long time.
Chondroitin sulfate is the key technology of mid-term preservation liquid development, it is considered to be one kind can be to extension under the conditions of 4 DEG C
The ingredient that the preservation of cornea time plays a crucial role, and this kind of preservation liquid there are the problem of one of be that each layer of cornea can absorb small point
Son amount chondroitin sulfate, so that moving the water to flow into matrix leads to corneal edema.But the addition of dextran then overcomes disadvantages mentioned above,
The loss for facilitating proteoglycan in reduction hypothallus, to maintain the transparency of cornea tissue.
Sodium chondroitin sulfate, Sodium Hyaluronate and Dextran 40 molecular structure contain a large amount of hydrophilic polar group,
Hydrogen bond association can be formed with hydrone, polymer molecule, so that cornea tissue, high molecular polymer and hydrone be made to organically combine
Together.In addition, sodium chondroitin sulfate and hyaluronic acid sodium molecule and cornea have special affinity, referred to as bio-adhesive
(Bioadhesion), good biological stretching can be presented in the cornea being processed.Therefore, sodium chondroitin sulfate, hyalomitome
The compounding combination of sour sodium, dextran, generated synergistic function are particularly conducive to maintain corneal lens original for a long time
Collagen fiber structure and the transparency.
The utility model has the advantages that the invention has the advantages that cooperateing with answering for Sodium Hyaluronate and dextran using sodium chondroitin sulfate
With system, the preservation liquid that can be used for cornea tissue long-term preservation is obtained, is particularly conducive to maintain the original glue of corneal lens for a long time
Fibrillar structure and the transparency；After the long-term preservation liquid cornea preservation tissue, rewarming be can be used directly, easy to operate, is not required to
Rehydration.
Detailed description of the invention
Fig. 1 is in specific embodiment, for verifying the porcine cornea of preservation effect, the 0th day outside drawing；
Fig. 2 (a)-(c) is, for verifying the porcine cornea of preservation effect, to use long-term guarantor of the invention in specific embodiment
Liquid storage saves 1 month, 6 months, 12 months outside drawings respectively；
Fig. 3 (a)-(c) is, for verifying the porcine cornea of preservation effect, to save liquid point using control in specific embodiment
It Bao Cun not 1 month, 6 months, 12 months outside drawings；
Fig. 4 is in specific embodiment, for verifying the porcine cornea of preservation effect, the 0th day histotomy microscope figure；
Fig. 5 (a)-(c) is, for verifying the porcine cornea of preservation effect, to use long-term guarantor of the invention in specific embodiment
Liquid storage saves 1 month, 6 months, 12 months histotomy microscope figures respectively；
Fig. 6 (a)-(c) is, for verifying the porcine cornea of preservation effect, to save liquid point using control in specific embodiment
It Bao Cun not 1 month, 6 months, 12 months histotomy microscope figures；
The microscope magnifications of Fig. 4-Fig. 6 be 200 ×.
Specific embodiment
In the following with reference to the drawings and specific embodiments, the present invention is furture elucidated, these embodiments are merely to illustrate the present invention
Rather than it limits the scope of the invention.
Embodiment 1
A kind of cornea tissue long-term preservation liquid, including following components and content:
The preparation method of above-mentioned cornea tissue long-term preservation liquid, specifically includes the following steps:
Step S10: it weighs: weighing each component respectively according to formula ratio.
Step S20: it prepares: including:
Step S201: being added partial syringe water in container, control 59 ± 1 DEG C of water temperature, be then respectively adding chondroitin sulfate
Plain sodium, Sodium Hyaluronate, Dextran 40, Sodium Pyruvate, sodium bicarbonate, 4- hydroxyethyl piperazineethanesulfonic acid, then fill into injection
Water simultaneously stirs 40min, dissolves the above component；
Step S202: the mixed liquor of step S201 is controlled 39 ± 1 DEG C of temperature, vitamin C is added, adds partial syringe
With water and stir 10min；
Step S203: the mixed liquor of step S202 is controlled 39 ± 1 DEG C of temperature, gentamicin sulphate is added, adds portion
Divide water for injection and stirs 10min；
Step S204: the mixed liquor of step S203 is controlled 39 ± 1 DEG C of temperature, glycerol is added, adds the injection of surplus
With water constant volume and 20min is stirred, obtains intermediate product.
Step S30: intermediate product examine is surveyed: being sampled to intermediate product, is carried out pH detection and appearance detection, pH detects qualified index and is
7.2~7.6, it is colourless to yellowish clear liquid that appearance, which detects qualified index, and no suspended substance, without precipitating, such as pH is detected not
Meet, is then adjusted using hydrochloric acid or sodium hydroxide solution, do not meet still, scrap after adjusting.
Step S40: aseptic filtration: advanced by step S30 detection qualified intermediate product or qualified intermediate product after adjusting
Row pre-filtering, then be filtered by 0.2 μm of PES filter to get the cornea tissue long-term preservation liquid.
Can be filling using low borosilicate ampoule bottle or middle borosilicate ampoule bottle by long-term preservation liquid obtained, and jumped a queue and roll lid
It is closed.
Embodiment 2
A kind of cornea tissue long-term preservation liquid, including following components and content:
The preparation method of above-mentioned cornea tissue long-term preservation liquid, substantially the same manner as Example 1, difference is: step
In S201,50 ± 1 DEG C of water temperature are controlled, stirs 80min；In step S202,35 ± 1 DEG C of temperature are controlled, stirs 20min；Step
In S203,35 ± 1 DEG C of temperature are controlled, stirs 15min；In step S204,35 ± 1 DEG C of temperature are controlled, stirs 20min.
Embodiment 3
A kind of cornea tissue long-term preservation liquid, including following components and content:
The preparation method of above-mentioned cornea tissue long-term preservation liquid, substantially the same manner as Example 1, difference is: step
In S201,40 ± 1 DEG C of water temperature are controlled, stirs 100min；In step S202,35 ± 1 DEG C of temperature are controlled, stirs 30min；Step
In S203,35 ± 1 DEG C of temperature are controlled, stirs 25min；In step S204,35 ± 1 DEG C of temperature are controlled, stirs 25min.
Embodiment 4
A kind of cornea tissue long-term preservation liquid, including following components and content:
The preparation method of above-mentioned cornea tissue long-term preservation liquid, it is same as Example 1.
Embodiment 5
A kind of cornea tissue long-term preservation liquid, including following components and content:
The preparation method of above-mentioned cornea tissue long-term preservation liquid, it is same as Example 1.
In the following, saving liquid using long-term preservation of the present invention liquid made from embodiment 3 and conventional aseptic glycerol as control, pass through
Porcine cornea verifies the preservation effect of cornea tissue long-term preservation liquid of the present invention.
Steps are as follows for the processing and preservation of porcine cornea piece:
Step 1: winning pig eyeball at slaughterhouse scene, rinsed with antibiotic sterile phosphate buffer；
Step 2: eyeball sclera portion is wrapped up with the wet yarn block containing antibiotic solution, to maintain the saturated with moisture in eyeball bottle
Environment (gauze piece must not contact cornea), cornea is put into eyeball bottle upwardly, drips antibiotic solution, lid to cornea face
Tightly；
Step 3: eyeball bottle is put into the transport case of ice cube, it need to be every avoiding ice cube temperature with material between eyeball bottle and ice cube
Spend it is low make cornea frostbite, temperature maintains 2~8 DEG C；
Step 4: after transporting laboratory back, eyeball is taken out on superclean bench, is fixed by tooth tweezer, with 10mm trepan in cornea
Surface rotates left and right, and does vertical drilling；After confirmation corneoscleral junction is kept completely separate with choroid below, corneal film is clamped
Limbus of sclera gently takes out, and is rinsed with antibiotic sterile phosphate buffer；
Step 5: by the corneal film after flushing be respectively put into the cryopreservation tube containing long-term preservation liquid of the present invention neutralize containing pair
According in the cryopreservation tube for saving liquid, it is totally submerged and saves liquid in long-term preservation liquid of the present invention and control, cover tightly bottle cap immediately, post mark
After label, it is put into program temperature reduction box and cools down, be finally put into liquid nitrogen and save；
Step 6: respectively after the 0th day, 1 month, 6 months and 12 months saves,
It is removed from liquid nitrogen the corneal film being stored in long-term preservation liquid of the present invention, is put into the constant temperature that temperature is 37 ± 2 DEG C
Rewarming 30min in shaking water bath pot then takes out and carries out appearance and histotomy Indexs measure；
It is removed from liquid nitrogen the corneal film being stored in control preservation liquid, is put into the isothermal vibration water that temperature is 37 ± 2 DEG C
Then rewarming 30min in bath rinses corneal film using sterile phosphate buffer, is put into phosphate isotonic buffer medium later
(sulfur acid chondroitin and dextran) 8~25min of rehydration then takes out and carries out appearance and histotomy Indexs measure.
1, appearance detects: corneal film is placed under natural light or fluorescent lamp, its appearance of visual observation:
By attached drawing 1 and attached drawing 2 (a)-(c) as it can be seen that under the conditions of liquid nitrogen, angle is saved respectively using long-term preservation liquid of the present invention
Diaphragm 1 month, 6 months, 12 months, corneal film appearance be center it is smooth, smooth, transparent, without oedema and turbid phenomenon, with the
0 day fresh cornea appearance indifference；
By attached drawing 1 with attached drawing 3 (a)-(c) as it can be seen that and use compares preservation liquid cornea preservation piece 1 month, appearance moderate hair
Huang, slight oedema thicken and muddy, and after 6 months, 12 months, the obvious oedema of corneal film is thickened and muddy.
2, histotomy detects:
By attached drawing 4 and attached drawing 5 (a)-(c) as it can be seen that microscopically observation uses long-term guarantor of the invention under the conditions of liquid nitrogen
Liquid storage distinguishes cornea preservation piece 1 month, 6 months, 12 months, and hypothallus is without thickening and oedema, hypothallus Collagen fiber row
Column are neat close, and diameter is uniform, without branch, and epithelial layer and endothelial layer connect between each level of cornea tissue substantially without missing
Closely；
By attached drawing 4 with attached drawing 6 (a)-(c) as it can be seen that and use compare save liquid cornea preservation piece after, hypothallus is different degrees of
It thickens, be broken, between epithelial layer and endothelial layer and its organized layer adhered to, also occur being detached from various degree, lack.
Under the conditions of liquid nitrogen, after long-term preservation liquid cornea preservation tissue of the invention, rewarming be can be used directly, operation letter
It is single, it is not required to rehydration.And after using conventional aseptic glycerol cornea preservation tissue, cornea when in use, need to answer cornea tissue
Mild rehydration.Long-term preservation liquid of the invention can be used for cornea tissue long-term preservation, include total corneal, corneal lens and plate layer angle
Film.
Claims (7)
1. a kind of cornea tissue long-term preservation liquid, it is characterised in that including following components and content:
2. cornea tissue long-term preservation liquid according to claim 1, it is characterised in that: the pH value of the long-term preservation liquid is
7.2~7.6, appearance be colourless to yellowish clear liquid, and no suspended substance, without precipitating.
3. cornea tissue long-term preservation liquid according to claim 1, it is characterised in that: the bacterium endogenous toxic material of the water for injection
Plain < 0.25EU/ml.
4. a kind of preparation method of any cornea tissue long-term preservation liquid of claims 1 to 3, it is characterised in that including with
Lower step:
Step S10: it weighs: weighing each component respectively according to formula ratio；
Step S20: it prepares: including:
Step S201: being added partial syringe water in container, control 25~60 DEG C of water temperature, be then respectively adding chondroitin sulfate
Sodium, Sodium Hyaluronate, Dextran 40, Sodium Pyruvate, sodium bicarbonate, 4- hydroxyethyl piperazineethanesulfonic acid, then fill into water for injection
And stir 30~120min；
Step S202: controlling 16~40 DEG C of temperature for the mixed liquor of step S201, vitamin C be added, and adds partial syringe use
Water simultaneously stirs 5~30min；
Step S203: controlling 16~40 DEG C of temperature for the mixed liquor of step S202, gentamicin sulphate be added, and adds part note
It penetrates with water and stirs 5~30min；
Step S204: the mixed liquor of step S203 is controlled 16~40 DEG C of temperature, glycerol is added, adds the water for injection of surplus
Constant volume simultaneously stirs 10~30min, obtains intermediate product；
Step S30: intermediate product examine is surveyed: being sampled to intermediate product, is carried out pH detection and appearance detection, detection needs centering as do not met
Between product carry out pH adjusting, do not meet still, scrap after adjusting；
Step S40: aseptic filtration: step S30 detection qualified intermediate product or qualified intermediate product after adjusting first carry out pre-
Filtering, then be filtered by 0.2 μm of PES filter to get the cornea tissue long-term preservation liquid.
5. the preparation method according to claim 4, it is characterised in that: in step S30, pH detect qualified index be 7.2~
7.6, it is colourless to yellowish clear liquid that appearance, which detects qualified index, and no suspended substance, without precipitating.
6. the preparation method according to claim 4, it is characterised in that: in step S40, carry out pH to intermediate product and adjust use
Hydrochloric acid or sodium hydroxide solution.
7. the preparation method according to claim 4, it is characterised in that: the long-term preservation liquid using low borosilicate ampoule bottle or
Middle borosilicate ampoule bottle is filling, and jumped a queue and roll lid it is closed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811440809.0A CN109221093A (en) | 2018-11-29 | 2018-11-29 | A kind of cornea tissue long-term preservation liquid and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811440809.0A CN109221093A (en) | 2018-11-29 | 2018-11-29 | A kind of cornea tissue long-term preservation liquid and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109221093A true CN109221093A (en) | 2019-01-18 |
Family
ID=65073699
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811440809.0A Pending CN109221093A (en) | 2018-11-29 | 2018-11-29 | A kind of cornea tissue long-term preservation liquid and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109221093A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109769799A (en) * | 2019-03-20 | 2019-05-21 | 江苏瑞思坦生物科技有限公司 | A kind of human fat tissue saves liquid and preparation method thereof |
| CN111066777A (en) * | 2019-12-19 | 2020-04-28 | 镇江雷音再生医学科技有限公司 | Corneal lens ultralow-temperature long-term storage method |
| CN111685101A (en) * | 2019-03-11 | 2020-09-22 | 广东博与再生医学有限公司 | Preservation and transportation fluid for acellular lamellar cornea |
| CN111802378A (en) * | 2020-07-24 | 2020-10-23 | 镇江雷音再生医学科技有限公司 | SMILE (Small Scale Integrated Circuit) -derived protective solution for human corneal lens and preparation method thereof |
| US11154052B2 (en) | 2018-09-14 | 2021-10-26 | University Of Miami | Dual-chamber vial for corneal graft preservation |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4695536A (en) * | 1984-01-10 | 1987-09-22 | Lindstrom Richard L | Corneal storage system |
| CN1162389A (en) * | 1997-04-16 | 1997-10-22 | 王丽娅 | Liquid for preserving cornea for medium period |
| CN101502257A (en) * | 2009-03-13 | 2009-08-12 | 厦门大学 | Corneal midterm preservation solution and preparation method thereof |
| CN103190392A (en) * | 2013-03-28 | 2013-07-10 | 北京大学第三医院 | Cornea mid-term preserving fluid and preparation method thereof |
| CN106212442A (en) * | 2016-07-01 | 2016-12-14 | 山东省眼科研究所 | A kind of de-cell cornea stand-by protection liquid |
| EP3170391A1 (en) * | 2014-07-18 | 2017-05-24 | Youvision Biotech Co., Ltd. | Lamellar cornea preserving solution |
-
2018
- 2018-11-29 CN CN201811440809.0A patent/CN109221093A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4695536A (en) * | 1984-01-10 | 1987-09-22 | Lindstrom Richard L | Corneal storage system |
| CN1162389A (en) * | 1997-04-16 | 1997-10-22 | 王丽娅 | Liquid for preserving cornea for medium period |
| CN101502257A (en) * | 2009-03-13 | 2009-08-12 | 厦门大学 | Corneal midterm preservation solution and preparation method thereof |
| CN103190392A (en) * | 2013-03-28 | 2013-07-10 | 北京大学第三医院 | Cornea mid-term preserving fluid and preparation method thereof |
| EP3170391A1 (en) * | 2014-07-18 | 2017-05-24 | Youvision Biotech Co., Ltd. | Lamellar cornea preserving solution |
| CN106212442A (en) * | 2016-07-01 | 2016-12-14 | 山东省眼科研究所 | A kind of de-cell cornea stand-by protection liquid |
Non-Patent Citations (3)
| Title |
|---|
| 范军华 等: "角膜保存的现状与展望", 《国际眼科杂志》 * |
| 蒋华 等: "透明质酸钠在甘油冷冻保存角膜中的应用", 《解放军医学杂志》 * |
| 邱姣 等: "硫酸软骨素在长期甘油冷冻保存角膜中对内皮细胞活性的影响", 《广东医学》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11154052B2 (en) | 2018-09-14 | 2021-10-26 | University Of Miami | Dual-chamber vial for corneal graft preservation |
| CN111685101A (en) * | 2019-03-11 | 2020-09-22 | 广东博与再生医学有限公司 | Preservation and transportation fluid for acellular lamellar cornea |
| CN109769799A (en) * | 2019-03-20 | 2019-05-21 | 江苏瑞思坦生物科技有限公司 | A kind of human fat tissue saves liquid and preparation method thereof |
| CN111066777A (en) * | 2019-12-19 | 2020-04-28 | 镇江雷音再生医学科技有限公司 | Corneal lens ultralow-temperature long-term storage method |
| CN111802378A (en) * | 2020-07-24 | 2020-10-23 | 镇江雷音再生医学科技有限公司 | SMILE (Small Scale Integrated Circuit) -derived protective solution for human corneal lens and preparation method thereof |
| CN111802378B (en) * | 2020-07-24 | 2022-02-08 | 镇江雷音再生医学科技有限公司 | SMILE (Small Scale Integrated Circuit) -derived protective solution for human corneal lens and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109221093A (en) | A kind of cornea tissue long-term preservation liquid and preparation method thereof | |
| CN101502257B (en) | Corneal midterm preservation solution and preparation method thereof | |
| CA1187799A (en) | Ophthalmic irrigating solution | |
| Jacob et al. | Preliminary evidence of successful near vision enhancement with a new technique: PrEsbyopic Allogenic Refractive Lenticule (PEARL) corneal inlay using a SMILE lenticule | |
| EP2830627B1 (en) | Ocular treatment solutions, delivery devices and delivery augmentation methods | |
| CN103269686B (en) | Contact lens ophthalmic composition | |
| EP0205279A1 (en) | Non-toxic ophthalmic preparations and methods of preparing them | |
| CN106421908B (en) | A kind of preparation method of de- cell cornea | |
| CN102697713B (en) | Sodium hyaluronate eye drops and a preparation method thereof | |
| Vazirani et al. | Autologous serum eye drops in dry eye disease: Preferred practice pattern guidelines | |
| Hu et al. | Recent advances in small incision lenticule extraction (SMILE)-derived refractive lenticule preservation and clinical reuse | |
| CN104338181B (en) | A kind of method for removing cells of people's corneal stroma | |
| CN102357243B (en) | Recombinant cattle basic fibroblastic growth factor eye drops | |
| CN111066777A (en) | Corneal lens ultralow-temperature long-term storage method | |
| CN105617350A (en) | Extraction method of sheep sclera collagen, ophthalmic gel and preparation method thereof | |
| Kim et al. | Tissue adhesives in corneal cataract incisions | |
| CN106265744A (en) | Umbilical cord mesenchymal stem cells application in the stem cell medicine of preparation treatment corneal injury | |
| Martinez et al. | Endothelial graft failure after contralateral autologous corneal transplantation | |
| RU1803110C (en) | Method of ophthalmic drops preparation | |
| CN111657269B (en) | Method for protecting and treating fibrin glue before preservation of SMILE-derived human corneal lens | |
| CN102961399B (en) | Sodium chloride eye drops and preparation method thereof | |
| Van Rij et al. | Toxic keratopathy due to the accidental use of chlorhexidine, cetrimide and cialit | |
| CN107115299B (en) | Rapamycin nano micelle eye drops and preparation method thereof | |
| CN104606666A (en) | Recombinant bovine alkaline fibroblast growth factor eye drops | |
| CN111802378B (en) | SMILE (Small Scale Integrated Circuit) -derived protective solution for human corneal lens and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190118 |
|
| RJ01 | Rejection of invention patent application after publication |