CN109187935A - A method of Ancient Silk Textile is detected based on microwave - Google Patents
A method of Ancient Silk Textile is detected based on microwave Download PDFInfo
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- CN109187935A CN109187935A CN201811055063.1A CN201811055063A CN109187935A CN 109187935 A CN109187935 A CN 109187935A CN 201811055063 A CN201811055063 A CN 201811055063A CN 109187935 A CN109187935 A CN 109187935A
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- 238000000034 method Methods 0.000 title claims abstract description 25
- 239000004753 textile Substances 0.000 title claims abstract description 18
- 108010022355 Fibroins Proteins 0.000 claims abstract description 48
- 238000001514 detection method Methods 0.000 claims abstract description 28
- 239000002096 quantum dot Substances 0.000 claims abstract description 20
- ZCCIPPOKBCJFDN-UHFFFAOYSA-N calcium nitrate Chemical compound [Ca+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ZCCIPPOKBCJFDN-UHFFFAOYSA-N 0.000 claims abstract description 18
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 claims abstract description 18
- 235000008708 Morus alba Nutrition 0.000 claims abstract description 15
- 240000000249 Morus alba Species 0.000 claims abstract description 15
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 claims abstract description 9
- 235000019253 formic acid Nutrition 0.000 claims abstract description 9
- 239000000872 buffer Substances 0.000 claims description 49
- 239000000243 solution Substances 0.000 claims description 27
- 238000002965 ELISA Methods 0.000 claims description 25
- 239000007788 liquid Substances 0.000 claims description 25
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 25
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 21
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims description 20
- 239000008367 deionised water Substances 0.000 claims description 14
- 229910021641 deionized water Inorganic materials 0.000 claims description 14
- 238000003756 stirring Methods 0.000 claims description 12
- 238000005119 centrifugation Methods 0.000 claims description 11
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 10
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 10
- 238000011534 incubation Methods 0.000 claims description 10
- 239000013642 negative control Substances 0.000 claims description 10
- 229910000029 sodium carbonate Inorganic materials 0.000 claims description 10
- 239000003643 water by type Substances 0.000 claims description 10
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims description 8
- 239000000843 powder Substances 0.000 claims description 8
- 108091003079 Bovine Serum Albumin Proteins 0.000 claims description 7
- 229940098773 bovine serum albumin Drugs 0.000 claims description 7
- UHYPYGJEEGLRJD-UHFFFAOYSA-N cadmium(2+);selenium(2-) Chemical class [Se-2].[Cd+2] UHYPYGJEEGLRJD-UHFFFAOYSA-N 0.000 claims description 7
- 238000000502 dialysis Methods 0.000 claims description 7
- 239000012460 protein solution Substances 0.000 claims description 7
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 6
- 239000011734 sodium Substances 0.000 claims description 6
- 229910052708 sodium Inorganic materials 0.000 claims description 6
- 235000017557 sodium bicarbonate Nutrition 0.000 claims description 6
- LMDZBCPBFSXMTL-UHFFFAOYSA-N 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide Chemical compound CCN=C=NCCCN(C)C LMDZBCPBFSXMTL-UHFFFAOYSA-N 0.000 claims description 5
- 239000001913 cellulose Substances 0.000 claims description 5
- 229920002678 cellulose Polymers 0.000 claims description 5
- 229920001577 copolymer Polymers 0.000 claims description 5
- 230000005284 excitation Effects 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 5
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 5
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 5
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 5
- 229920003229 poly(methyl methacrylate) Polymers 0.000 claims description 5
- 239000004926 polymethyl methacrylate Substances 0.000 claims description 5
- 239000013641 positive control Substances 0.000 claims description 5
- 239000001103 potassium chloride Substances 0.000 claims description 5
- 235000011164 potassium chloride Nutrition 0.000 claims description 5
- 239000002244 precipitate Substances 0.000 claims description 5
- 238000002360 preparation method Methods 0.000 claims description 5
- 238000012545 processing Methods 0.000 claims description 5
- 239000011780 sodium chloride Substances 0.000 claims description 5
- 239000006228 supernatant Substances 0.000 claims description 5
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical class [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims description 4
- 238000000746 purification Methods 0.000 claims description 4
- 239000002253 acid Substances 0.000 claims description 2
- 238000004108 freeze drying Methods 0.000 claims description 2
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims description 2
- 238000009777 vacuum freeze-drying Methods 0.000 claims description 2
- 238000005406 washing Methods 0.000 claims description 2
- DAJSVUQLFFJUSX-UHFFFAOYSA-M sodium;dodecane-1-sulfonate Chemical compound [Na+].CCCCCCCCCCCCS([O-])(=O)=O DAJSVUQLFFJUSX-UHFFFAOYSA-M 0.000 claims 1
- 230000035945 sensitivity Effects 0.000 abstract description 8
- 241000255789 Bombyx mori Species 0.000 abstract description 5
- 239000004744 fabric Substances 0.000 abstract description 5
- 230000000694 effects Effects 0.000 abstract description 4
- 238000005303 weighing Methods 0.000 description 4
- 241000283690 Bos taurus Species 0.000 description 3
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 3
- 238000013019 agitation Methods 0.000 description 3
- 239000007864 aqueous solution Substances 0.000 description 3
- 238000011010 flushing procedure Methods 0.000 description 3
- 229910052739 hydrogen Inorganic materials 0.000 description 3
- 239000001257 hydrogen Substances 0.000 description 3
- 238000000643 oven drying Methods 0.000 description 3
- 210000002966 serum Anatomy 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- BVKZGUZCCUSVTD-UHFFFAOYSA-M Bicarbonate Chemical class OC([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-M 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 150000001661 cadmium Chemical class 0.000 description 2
- BVKZGUZCCUSVTD-UHFFFAOYSA-N carbonic acid Chemical compound OC(O)=O BVKZGUZCCUSVTD-UHFFFAOYSA-N 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 150000002431 hydrogen Chemical class 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 230000010355 oscillation Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 description 1
- QXNVGIXVLWOKEQ-UHFFFAOYSA-N Disodium Chemical compound [Na][Na] QXNVGIXVLWOKEQ-UHFFFAOYSA-N 0.000 description 1
- SNRUBQQJIBEYMU-UHFFFAOYSA-N Dodecane Natural products CCCCCCCCCCCC SNRUBQQJIBEYMU-UHFFFAOYSA-N 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 125000003438 dodecyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 239000011368 organic material Substances 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 229910052711 selenium Inorganic materials 0.000 description 1
- 239000011669 selenium Substances 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
- 238000009941 weaving Methods 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/36—Textiles
- G01N33/365—Filiform textiles, e.g. yarns
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/588—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with semiconductor nanocrystal label, e.g. quantum dots
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- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Biochemistry (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Textile Engineering (AREA)
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- Peptides Or Proteins (AREA)
Abstract
The present invention relates to historical relic detection fields, disclose a kind of method based on microwave detection Ancient Silk Textile.The present invention first uses calcium nitrate, formic acid system to prepare Bombyx mori silk fibroin, then it is prepared for a kind of fibroin albumen antibody of quantum dot pearl label, fibroin albumen and silk fabric cultural relics sample are subjected to microwave Elisa detection therewith, can determine whether contain mulberry silk in historical relic sample by fluorescence intensity level.Amount of samples of the present invention is few, has quickly, accurately, the characteristics of high sensitivity, has good detection effect to the silk goods to addle.
Description
Technical field
The present invention relates to historical relic detection field more particularly to a kind of methods based on microwave detection Ancient Silk Textile.
Background technique
China is exactly textile big country since ancient times, and the textile type of production is abundant, and technique is exquisite, comfortable ventilating.Its
In most well-known textile be exactly China silk, therefore China is otherwise known as " state of silk ".The main component of silk is mulberry
Silk, mulberry silk are mainly made of fibroin albumen and silk gum two parts, and fibroin albumen is the chief component of silk, are accounted for about total
The 70% of weight.And mulberry silk, as a kind of high-molecular organic material, the influence vulnerable to light, heat, soda acid, microorganism etc. occurs
Degradation, so that the variation of the structures such as crystallinity, molecular weight and performance is caused, so conventional detection method sensitivity is low, by miscellaneous
Matter interference effect is big, is not suitable for detecting historical relic, it is therefore desirable to develop that a kind of sensitivity is good, the detection silk weaving of high specificity
The method of product.
Microwave detection has the characteristics that high sensitivity, easy to operate, is widely used in various fields.But due to this
Invent it is to be detected for silk fabric cultural relics, it is different from conventional detection sample, it is therefore necessary to specifically for silk fabric cultural relics this
One special object improves microwave detection method.
Summary of the invention
In order to solve the above-mentioned technical problems, the present invention provides a kind of methods based on microwave detection Ancient Silk Textile.This
Invention is directed to this special test object of silk fabric cultural relics sample, has carried out many improvement to technique.Utilize the method for the present invention pair
Ancient Silk Textile is detected, and has the characteristics that intuitive, accurate, high sensitivity.
The specific technical proposal of the invention is: a kind of method based on microwave detection Ancient Silk Textile, in terms of μ g, g and mL,
The following steps are included:
A it) weighs 4-6g mulberry silk to be placed in sodium carbonate liquor of the 180-220mL containing 0.018-0.022M, water-bath 55-65min, water
Bath temperature is 75-85 DEG C, and taking-up is cleaned more than three times with deionized water, dry, obtains fibroin.
B formic acid 48-52mL is added in the fibroin for) taking 1.8-2.2g to dry, 2.3-2.7g calcium nitrate, stirs 80-100min,
Filtering addition sodium bicarbonate is in neutrality up to solution, and after dialysis freeze-drying, obtained fibroin albumen is ground into fibroin albumen
Powder, it is spare.
The present invention dissolves fibroin using calcium nitrate, formic acid system, not only can increase the solubility to fibroin, but also can reduce to silk
The destruction of plain strand, and the dissolution to fibroin can be completed in the system at normal temperature, without heating.
C 18-22mg cadmium selenide/ZnS quantum dots) are weighed, 118-122mg polymethyl methacrylate, 78-82mg's
The dodecyl into the chloroform of 1.8-2.2ml, with the 3mg/ml of 4.5-5.5ml is added in polymaleic anhydride-octadecylene copolymer
The mixing of sulfonic acid sodium water solution, the processing of ultrasonic wave homogenizing, then by chloroform evaporated；Then gained water-soluble quantum dot pearl is centrifuged pure
Change, cleans 2-4 times with deionized water and obtain quantum dot pearl.
Quantum dot pearl prepared by the present invention includes many cadmium selenide/ZnS quantum dots, and luminous intensity is single selenizing
Thousands of times of cadmium/ZnS quantum dots, plays the role of fluorescence signal amplification in the detection process, increase the sensitivity of detection.
D 1- (3- dimethylamino-propyl) -3- ethyl carbodiimide of 0.9-1.1 μ g, the step C of 0.1-0.14mg) are taken) institute
It obtains water-soluble quantum dot pearl to be added in 7.4 buffer of PBS of 2.5-3.1ml, 90-110 μ l is added dropwise when being slowly stirred
It is diluted to 1000 times of fibroin albumen antibody with 1wt% bovine serum albumin, places 35-45min at room temperature, is centrifuged, taking precipitate
It is resuspended with 600 μ l PBS, 7.4 buffer, it is then spare at 1-5 DEG C.
E it) takes 0.02-0.2g historical relic sample to be dissolved in 9.6 buffer of CB of 100ml, is mixed evenly, stand, take 80-
120 μ l supernatants are added to ELISA Plate a column, by silk fibroin powder obtained by step B) with 9.6 buffer of CB at 100 μ g/ml
Silk fibroin protein solution, take 80-120 μ l be added to ELISA Plate b, c column, b column be used as positive control, c column be used as blank control, take
7.4 buffer of 80-120 μ l PBS is added to ELISA Plate d column as negative control, and ELISA Plate is placed in micro-wave oven is low and middle-grade to incubate
2-4min is educated, liquid in hole is sucked out, is washed with 7.4 buffer of PBS.
F confining liquid 180-220 μ l) is added in every hole, is placed in micro-wave oven and is incubated for 2-4min, liquid in hole is sucked out, uses PBS
The washing of 7.4 buffers.
G step D) is taken) acquired solution 80-120 μ l is added to ELISA Plate a, and in b, d column, confining liquid 80-120 μ l is taken to be added
It into c column, is placed in micro-wave oven and is incubated for 2-4min, liquid in hole is sucked out, is washed with 7.4 buffer of PBS.
It is compared with the traditional method, the present invention is incubated for using microwave promotes antigen-antibody to accelerate reaction by the higher-order of oscillation, makes
Experimental period greatly shortens, and improves the repeatability of testing result.
H fluorescent value) is measured using fluorescence microplate reader, using+3 SD of OD mean value of negative control as cut-off value, if a
The cut-off value of the fluorescence average value > d column of column, then determine to contain mulberry silk in historical relic sample.
The present invention first uses calcium nitrate, formic acid system to prepare Bombyx mori silk fibroin, is then prepared for a kind of quantum dot pearl mark
Fibroin albumen and silk fabric cultural relics sample are carried out microwave detection therewith, pass through fluorescence intensity level by the fibroin albumen antibody of note
Judge whether contain mulberry silk in historical relic sample.Amount of samples of the present invention is few, has quickly, accurately, the characteristics of high sensitivity, to corruption
Bad silk goods have good detection effect.
Preferably, step A) in, the cellulose dialysis bag that acquired solution is 8000-10000 with molecular cut off is being gone
It dialyses 2-3 days in ionized water, and changes a water every 5-7h, by silk fibroin protein solution vacuum freeze drying 2-3 days.
Preferably, step C) in, centrifugation rate 8000-12000rpm, centrifugation time 8-12min.
Preferably, step E) in, 9.6 buffer method of CB are as follows: weigh 1.5 g sodium carbonate and 2.9 g bicarbonates
Sodium is added to uniform stirring in 800 mL deionized waters until with volumetric flask constant volume to 1000 mL after being completely dissolved, and adjusts solution
PH to 9.6.
Preferably, step D) and step E) in, the preparation method of 7.4 buffer of PBS are as follows: 0.2 g potassium chloride is weighed,
It is straight that 0.27 g potassium dihydrogen phosphate, 8 g sodium chloride and 1.42 g disodium hydrogen phosphates are added to uniform stirring in 800 mL deionized waters
To after being completely dissolved with volumetric flask constant volume to 1000 mL, the pH to 7.4 of solution is adjusted.
Preferably, step F) in, the confining liquid is 1wt% bovine serum albumin.
Preferably, step E) step F) step G) in, it is washed 3-5 times with 7.4 buffer of PBS, each 2-4min.
Preferably, step H) in, when fluorescence microplate reader detects fluorescence, excitation wavelength 360nm, launch wavelength is
500nm。
It is compared with the prior art, the beneficial effects of the present invention are:
(1) present invention can dissolve fibroin using calcium nitrate, formic acid system at normal temperature, both can increase the solubility to fibroin,
The destruction to fibroin strand can be reduced again.
(2) quantum dot pearl prepared by the present invention includes many cadmium selenide/ZnS quantum dots, and luminous intensity is single selenium
Thousands of times of cadmium/ZnS quantum dots, plays the role of fluorescence signal amplification in the detection process, increase the sensitive of detection
Degree.
(3) microwave used in the present invention promotes antigen-antibody to accelerate reaction by the higher-order of oscillation, makes experimental period significantly
Shorten, and improves the repeatability of testing result.
(4) amount of samples of the present invention is few, has quickly, accurately, the characteristics of high sensitivity, has well to the silk goods to addle
Detection effect.
Specific embodiment
The present invention will be further described with reference to the examples below.
Embodiment 1:
A it) weighs 5g mulberry silk to be placed in sodium carbonate liquor of the 180mL containing 0.018M, water-bath 55min, bath temperature is 80 DEG C, is taken
It is cleaned more than three times with deionized water out, is put into oven drying.
B formic acid 48mL is added in the fibroin for) taking 2g to dry, 2.3g calcium nitrate, and with magnetic agitation 80min, carbonic acid is added in filtering
Hydrogen sodium is dialysed 2 days up to solution is in neutrality with the cellulose dialysis bag that molecular cut off is 8000 in deionized water, and every
5h changes a water, and silk fibroin protein solution is freeze-dried 2 days in vacuum freeze drier, and obtained fibroin albumen is ground into
Powder is spare.
C 20mg cadmium selenide/ZnS quantum dots, 118mg polymethyl methacrylate, the polymaleic anhydride-of 78mg) are weighed
Octadecylene copolymer, is added in the chloroform of 1.8ml, uses after mixing with the sodium dodecyl sulfate aqueous solution of the 3mg/ml of 4.5ml
Ultrasonic homogenizer processing, later again by chloroform evaporated.Then by gained water-soluble quantum dot pearl centrifugal purification, centrifugation rate is
8000rpm, time 8min clean 2 times with deionized water and obtain quantum dot pearl.
D 1- (3- dimethylamino-propyl) -3- ethyl carbodiimide of 0.9 μ g, the step C of 0.1mg) are taken) in quantum dot
Pearl is added in 2.5 mlPBS, 7.4 buffer, and 90 μ l 1wt% cow's serum egg is then added dropwise while being slowly stirred
The white fibroin albumen antibody for being diluted to 1000 times, places 35min at room temperature, centrifugation, and taking precipitate is slow with 600 μ l PBS 7.4
Fliud flushing is resuspended, and is then stored in spare in 4 DEG C of refrigerators.
E it) takes 0.02g historical relic sample to be dissolved in 9.6 buffer of 100mlCB, is mixed evenly, stand, take 80 μ l supernatants
ELISA Plate a column are added to, by the Bombyx mori silk fibroin powder in step B) with 9.6 buffer of CB at the silk of 100 μ g/ml
Fibroin solution takes 80 μ l to be added to ELISA Plate b, c column, and b column are used as positive control, and c column are used as blank control, take 80 μ l PBS
7.4 buffers are added to ELISA Plate d column as negative control, and ELISA Plate is placed in the low and middle-grade incubation 2min of micro-wave oven, hole is sucked out
Interior liquid is washed 3 times, each 2min with 7.4 buffer of PBS.
9.6 buffer of CB: weighing 1.5 g sodium carbonate and 2.9 g sodium bicarbonates are added to 800 mL deionized waters
Middle uniform stirring adjusts the pH to 9.6 of solution until with volumetric flask constant volume to 1000 mL after being completely dissolved.
F 180 μ l of 1wt% bovine serum albumin) is added in every hole, is placed in the low and middle-grade incubation 2min of micro-wave oven, liquid in hole is sucked out
Body is washed 3 times, each 2min with 7.4 buffer of PBS.
G) take step D) in 80 μ l of solution be added to ELISA Plate a, in b, d column, 80 μ l of confining liquid is taken to be added in c column,
The low and middle-grade incubation 2min of micro-wave oven are placed in, liquid in hole is sucked out, is washed 3 times with 7.4 buffer of PBS, each 2min.
The preparation of 7.4 buffer of PBS: 0.2 g potassium chloride, 0.27 g potassium dihydrogen phosphate, 8 g sodium chloride and 1.42 are weighed
G disodium hydrogen phosphate is added to uniform stirring in 800 mL deionized waters until with volumetric flask constant volume to 1000 after being completely dissolved
ML adjusts the pH to 7.4 of solution.
H fluorescent value, excitation wavelength 360nm, launch wavelength 500nm, by negative control) are measured using fluorescence microplate reader
+ 3 SD of OD mean value as cut-off value, if the cut-off value of the fluorescence average value > d column of a column, illustrates in historical relic sample
Contain mulberry silk.
Embodiment 2:
A it) weighs 5g mulberry silk to be placed in sodium carbonate liquor of the 200mL containing 0.02M, water-bath 60min, bath temperature is 80 DEG C, is taken
It is cleaned more than three times with deionized water out, is put into oven drying.
B formic acid 50mL is added in the fibroin for) taking 2g to dry, 2.5g calcium nitrate, and with magnetic agitation 90min, carbonic acid is added in filtering
Hydrogen sodium is dialysed 2.5 days up to solution is in neutrality with the cellulose dialysis bag that molecular cut off is 9000 in deionized water, and every
A water is changed every 6h, silk fibroin protein solution is freeze-dried 2.5 days in vacuum freeze drier, obtained fibroin albumen is ground
Grinds are spare.
C 20mg cadmium selenide/ZnS quantum dots, 120mg polymethyl methacrylate, the polymaleic anhydride-of 80mg) are weighed
Octadecylene copolymer, is added in the chloroform of 2ml, with ultrasound after mixing with the sodium dodecyl sulfate aqueous solution of the 3mg/ml of 5ml
Wave homogenizer processing, later again by chloroform evaporated.Then by gained water-soluble quantum dot pearl centrifugal purification, centrifugation rate is
10000rpm, time 10min.3 times, which are cleaned, with deionized water obtains quantum dot pearl.
D 1- (3- dimethylamino-propyl) -3- ethyl carbodiimide of 1 μ g, the step C of 0.12mg) are taken) in quantum dot pearl
It is added in 7.4 buffer of 2..8mlPBS, 100 μ l 1wt% cow's serum egg is then added dropwise while being slowly stirred
The white fibroin albumen antibody for being diluted to 1000 times, places 40min at room temperature, centrifugation, and taking precipitate is slow with 600 μ l PBS 7.4
Fliud flushing is resuspended, and is then stored in spare in 4 DEG C of refrigerators.
E it) takes 0.11g historical relic sample to be dissolved in 9.6 buffer of 100mlCB, is mixed evenly, stand, take 100 μ l supernatants
Liquid is added to ELISA Plate a column, by the Bombyx mori silk fibroin powder in step B) with 9.6 buffer of CB at 100 μ g/ml's
Silk fibroin protein solution takes 100 μ l to be added to ELISA Plate b, c column, and b column are used as positive control, and c column are used as blank control, take 100 μ l
7.4 buffer of PBS is added to ELISA Plate d column as negative control, and ELISA Plate is placed in the low and middle-grade incubation 3min of micro-wave oven, is inhaled
Portal interior liquid, is washed 4 times with 7.4 buffer of PBS, each 3min.
9.6 buffer of CB: weighing 1.5 g sodium carbonate and 2.9 g sodium bicarbonates are added to 800 mL deionized waters
Middle uniform stirring adjusts the pH to 9.6 of solution until with volumetric flask constant volume to 1000 mL after being completely dissolved.
F 200 μ l of 1wt% bovine serum albumin) is added in every hole, is placed in the low and middle-grade incubation 3min of micro-wave oven, liquid in hole is sucked out
Body is washed 4 times, each 3min with 7.4 buffer of PBS.
G) take step D) in 100 μ l of solution be added to ELISA Plate a, in b, d column, 100 μ l of confining liquid is taken to be added to c column
In, the low and middle-grade incubation 3min of micro-wave oven are placed in, liquid in hole is sucked out, is washed 4 times with 7.4 buffer of PBS, each 3min.
The preparation of 7.4 buffer of PBS: 0.2 g potassium chloride, 0.27 g potassium dihydrogen phosphate, 8 g sodium chloride and 1.42 are weighed
G disodium hydrogen phosphate is added to uniform stirring in 800 mL deionized waters until with volumetric flask constant volume to 1000 after being completely dissolved
ML adjusts the pH to 7.4 of solution.
H fluorescent value, excitation wavelength 360nm, launch wavelength 500nm, by negative control) are measured using fluorescence microplate reader
+ 3 SD of OD mean value as cut-off value, if the cut-off value of the fluorescence average value > d column of a column, illustrates in historical relic sample
Contain mulberry silk.
Embodiment 3:
A it) weighing 5g mulberry silk to be placed in sodium carbonate liquor of the 220mL containing 0.022M, water-bath 65min, bath temperature is 80 DEG C,
Taking-up is cleaned more than three times with deionized water, is put into oven drying；
B formic acid 52mL is added in the fibroin for) taking 2g to dry, 2.7g calcium nitrate, and with magnetic agitation 100min, bicarbonate is added in filtering
Sodium is dialysed 3 days up to solution is in neutrality with the cellulose dialysis bag that molecular cut off is 10000 in deionized water, and every 7h
A water is changed, silk fibroin protein solution is freeze-dried 3 days in vacuum freeze drier, obtained fibroin albumen is pulverized
It is spare；
C 20mg cadmium selenide/ZnS quantum dots, 122mg polymethyl methacrylate, the polymaleic anhydride-ten of 82mg) are weighed
Eight alkene copolymers, are added in the chloroform of 2.2ml, with super after mixing with the sodium dodecyl sulfate aqueous solution of the 3mg/ml of 5.5ml
Sound wave homogenizer processing, later again by chloroform evaporated.Then by gained water-soluble quantum dot pearl centrifugal purification, centrifugation rate is
12000rpm, time 12min.4 times, which are cleaned, with deionized water obtains quantum dot pearl；
D 1- (3- dimethylamino-propyl) -3- ethyl carbodiimide of 1.1 μ g, the step C of 0.1-0.14mg) are taken) in quantum dot
Pearl is added in 7.4 buffer of 3.1mlPBS, and 110 μ l 1wt% cow's serum egg is then added dropwise while being slowly stirred
The white fibroin albumen antibody for being diluted to 1000 times, places 45min at room temperature, centrifugation, and taking precipitate is slow with 600 μ l PBS 7.4
Fliud flushing is resuspended, and is then stored in spare in 4 DEG C of refrigerators；
E it) takes 0.2g historical relic sample to be dissolved in 9.6 buffer of 100mlCB, is mixed evenly, stand, 120 μ l supernatants is taken to be added
It is arranged to ELISA Plate a, by the Bombyx mori silk fibroin powder in step B) with 9.6 buffer of CB at the fibroin egg of 100 μ g/ml
White solution takes 120 μ l to be added to ELISA Plate b, c column, and b column are used as positive control, and c column are used as blank control, take 120 μ l PBS
7.4 buffers are added to ELISA Plate d column as negative control, and ELISA Plate is placed in the low and middle-grade incubation 4min of micro-wave oven, hole is sucked out
Interior liquid is washed 5 times, each 4min with 7.4 buffer of PBS；
9.6 buffer of CB: weighing 1.5 g sodium carbonate and 2.9 g sodium bicarbonates are added in 800 mL deionized waters
Even stirring adjusts the pH to 9.6 of solution until with volumetric flask constant volume to 1000 mL after being completely dissolved.
F 220 μ l of 1wt% bovine serum albumin) is added in every hole, is placed in the low and middle-grade incubation 4min of micro-wave oven, liquid in hole is sucked out
Body is washed 5 times, each 4min with 7.4 buffer of PBS；
G) take step D) in 120 μ l of solution be added to ELISA Plate a, in b, d column, take 120 μ l of confining liquid to be added in c column, set
In the low and middle-grade incubation 4min of micro-wave oven, liquid in hole is sucked out, is washed 5 times with 7.4 buffer of PBS, each 4min；
The preparation of 7.4 buffer of PBS: 0.2 g potassium chloride, 0.27 g potassium dihydrogen phosphate, 8 g sodium chloride and 1.42 g phosphorus are weighed
Sour disodium hydrogen is added to uniform stirring in 800 mL deionized waters until with volumetric flask constant volume to 1000 mL after being completely dissolved, and adjusts
Save the pH to 7.4 of solution.
H fluorescent value, excitation wavelength 360nm, launch wavelength 500nm, by negative control) are measured using fluorescence microplate reader
+ 3 SD of OD mean value as cut-off value, if the cut-off value of the fluorescence average value > d column of a column, illustrates in historical relic sample
Contain mulberry silk.
Raw materials used in the present invention, equipment is unless otherwise noted the common raw material, equipment of this field；In the present invention
Method therefor is unless otherwise noted the conventional method of this field.
The above is only presently preferred embodiments of the present invention, is not intended to limit the invention in any way, it is all according to the present invention
Technical spirit any simple modification, change and equivalent transformation to the above embodiments, still fall within the technology of the present invention side
The protection scope of case.
Claims (8)
1. a kind of method based on microwave detection Ancient Silk Textile, which is characterized in that in terms of μ g, g and mL, comprising the following steps:
A it) weighs 4-6g mulberry silk to be placed in sodium carbonate liquor of the 180-220mL containing 0.018-0.022M, water-bath 55-65min, water
Bath temperature is 75-85 DEG C, and taking-up is cleaned more than three times with deionized water, dry, obtains fibroin；
B formic acid 48-52mL is added in the fibroin for) taking 1.8-2.2g to dry, 2.3-2.7g calcium nitrate, stirs 80-100min, filtering
Addition sodium bicarbonate is in neutrality up to solution, and after dialysis freeze-drying, obtained fibroin albumen is ground into silk fibroin powder, standby
With；
C 18-22mg cadmium selenide/ZnS quantum dots, 118-122mg polymethyl methacrylate, the poly- horse of 78-82mg) are weighed
Carry out acid anhydrides-octadecylene copolymer, the dodecyl sodium sulfonate into the chloroform of 1.8-2.2ml, with the 3mg/ml of 4.5-5.5ml is added
Sodium water solution mixing, the processing of ultrasonic wave homogenizing, then by chloroform evaporated；Then it by gained water-soluble quantum dot pearl centrifugal purification, uses
Deionized water cleans 2-4 times and obtains quantum dot pearl；
D 1- (3- dimethylamino-propyl) -3- ethyl carbodiimide of 0.9-1.1 μ g, the step C of 0.1-0.14mg) are taken) gained water
Dissolubility quantum dot pearl is added in 7.4 buffer of PBS of 2.5-3.1ml, and 90-110 μ l use is added dropwise when being slowly stirred
1wt% bovine serum albumin is diluted to 1000 times of fibroin albumen antibody, places 35-45min at room temperature, and centrifugation, taking precipitate is used
600 μ l PBS, 7.4 buffer is resuspended, then spare at 1-5 DEG C；
E it) takes 0.02-0.2g historical relic sample to be dissolved in 9.6 buffer of CB of 100ml, is mixed evenly, stand, take 80-120 μ l
Supernatant is added to ELISA Plate a column, by silk fibroin powder obtained by step B) with 9.6 buffer of CB at the silk of 100 μ g/ml
Fibroin solution takes 80-120 μ l to be added to ELISA Plate b, c column, and b column are used as positive control, and c column are used as blank control, take 80-
120 μ l PBS, 7.4 buffer is added to ELISA Plate d column as negative control, and ELISA Plate is placed in the low and middle-grade incubation 2- of micro-wave oven
4min is sucked out liquid in hole, is washed with 7.4 buffer of PBS；
F confining liquid 180-220 μ l) is added in every hole, is placed in micro-wave oven and is incubated for 2-4min, liquid in hole is sucked out, with PBS 7.4
Buffer washing；
G step D) is taken) acquired solution 80-120 μ l is added to ELISA Plate a, and in b, d column, confining liquid 80-120 μ l is taken to be added to c column
In, it is placed in micro-wave oven and is incubated for 2-4min, liquid in hole is sucked out, is washed with 7.4 buffer of PBS；
H fluorescent value) is measured using fluorescence microplate reader, using+3 SD of OD mean value of negative control as cut-off value, if what a was arranged
The cut-off value of fluorescence average value > d column, then determine to contain mulberry silk in historical relic sample.
2. a kind of method based on microwave detection Ancient Silk Textile as described in claim 1, which is characterized in that step A) in,
The cellulose dialysis bag that acquired solution is 8000-10000 with molecular cut off is dialysed 2-3 days in deionized water, and every 5-
7h changed a water, by silk fibroin protein solution vacuum freeze drying 2-3 days.
3. a kind of method based on microwave detection Ancient Silk Textile as described in claim 1, which is characterized in that step C) in,
Centrifugation rate is 8000-12000rpm, centrifugation time 8-12min.
4. a kind of method based on microwave detection Ancient Silk Textile as described in claim 1, which is characterized in that step E) in,
9.6 buffer method of CB are as follows: weigh 1.5 g sodium carbonate and 2.9 g sodium bicarbonates are added in 800 mL deionized waters
Uniform stirring adjusts the pH to 9.6 of solution until with volumetric flask constant volume to 1000 mL after being completely dissolved.
5. a kind of method based on microwave detection Ancient Silk Textile as described in claim 1, which is characterized in that step D) and step
Rapid E) in, the preparation method of 7.4 buffer of PBS are as follows: weigh 0.2 g potassium chloride, 0.27 g potassium dihydrogen phosphate, 8 g sodium chloride
Uniform stirring in 800 mL deionized waters is added to 1.42 g disodium hydrogen phosphates until after being completely dissolved extremely with volumetric flask constant volume
1000 mL adjust the pH to 7.4 of solution.
6. a kind of method based on microwave detection Ancient Silk Textile as described in claim 1, which is characterized in that step F) in,
The confining liquid is 1wt% bovine serum albumin.
7. a kind of method based on microwave detection Ancient Silk Textile as described in claim 1, which is characterized in that step E) step
F) step G) in, it is washed 3-5 times with 7.4 buffer of PBS, each 2-4min.
8. a kind of method based on microwave detection Ancient Silk Textile as described in claim 1, which is characterized in that step H) in,
When fluorescence microplate reader detects fluorescence, excitation wavelength 360nm, launch wavelength 500nm.
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