CN109187875A - A kind of anti-mite effect testing method - Google Patents
A kind of anti-mite effect testing method Download PDFInfo
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- CN109187875A CN109187875A CN201811130577.9A CN201811130577A CN109187875A CN 109187875 A CN109187875 A CN 109187875A CN 201811130577 A CN201811130577 A CN 201811130577A CN 109187875 A CN109187875 A CN 109187875A
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Abstract
The invention belongs to anti-mite the field of test technology, specially a kind of anti-mite effect testing method that can be used for evaluating detergent product or the anti-mite effect of fabric product.The present invention carrys out the anti-mite effect of qualitative and/or quantitative assessment fabric or detergent by comparing the diffusion of culture front and back graininess acarid feed distribution area, easily operated, experimental period is short, does not need to carry out many and diverse artificial mite number statistics living, rapidly and efficiently, and it is reproducible.
Description
Technical field
The invention belongs to anti-mite the field of test technology more particularly to one kind can be used for evaluating detergent product or fabric product
The anti-mite effect testing method of anti-mite effect.
Background technique
Mite class is the general designation of all kinds in addition to tick class in Arthropoda Arachnoidea Acari, and figure is small, one
As at 0.5 millimeter or so.Mite class is widely distributed, and dust mite is main groups therein.Dust mite resides in pillow, bedding, carpet, fiber
Toy etc., with people's exuviae bits and mould etc. for food.Body, excreta and the ovum of mite have strong allergenicity, can cause
The diseases such as allergic rhinitis, asthma and allergic dermatitis seriously endanger human health.
Harm for acarid to human body, row expert mainly starts with from the fabric most frequent with human contact at present,
Mite-assistant fabrics product is developed, the harm of acarid is solved the problems, such as with this.At present to the anti-mite method of evaluating performance of mite-assistant fabrics product
Mainly have and drives mite method and suppression mite method.Regulation drives mite method suitable for institute in standard GB/T/T24253-2009 of textile field
There is textile product, suppression mite method is only applicable to the product infrequently washed.Detergent is as cleaning product used in everyday, for clear
All fabric products are washed, therefore drive mite method need to be used to carry out anti-mite effect assessment to it.
Mite evaluation method is driven in standard GB/T/T24253-2009 of textile field are as follows: 7 culture dishes are taken, by 1
Culture dish is placed on culture dish centered on center, remaining 6 culture dish is uniformly put in periphery, around center culture dish at petal-shaped
It sets, and respectively contacts two-by-two, contact position is clung with adhesive tape, is barricaded as bridge and is creeped for acarid；In 6 peripheral culture dishes respectively
Compartment of terrain is put into sample and control sample, is then put into 0.05g acarid feed in each peripheral culture dish center；In center culture dish
In be put into mite living as test test worm, after culture for 24 hours, count the work mite number in 6 peripheral culture dishes under the microscope.
Technical literature " test anti-mite fabric drive mite effectiveness new method " the study found that using standard GB/T/
When the drive mite evaluation method test of T24253-2009, following problems are found: 1, sample group examination parallel with 3 in blank control group
Test the poor repeatability of data；2, the region other than 7 culture dishes finds a large amount of acarids, and the work mite quantity in control group is bright
It shows on the low side, causes test result reliability low；3, the work mite quantity on some test specimen is more than the mite of corresponding blank control group
Borer population amount is added to 0.01% anti-mite agent with the sample and misfits.In addition, the present inventor in research process, it was also found that
The above device for driving mite evaluation method makes time-consuming, and is easy to tilt at adhesion, causes acarid can not be successfully and passes through, causes reality
Test failure；Count acarid number when, need under the microscope to periphery each culture dish surface and feed heap in mite population into
Pedestrian's work statistics, inefficiency.
Summary of the invention
The present invention provides a kind of reproducible, high reliablity, fastly for the above problem existing for existing drives mite evaluation method
The anti-mite effect of the fast anti-mite effect that can be used for qualitative and quantitative assessment detergent product or fabric product efficiently, easily to operate
Test method.
To achieve the above object, the present invention uses following technical scheme.
A kind of anti-mite effect testing method, comprising the following steps:
S10, fabric to be tested is placed in inner wall coated in the first culture dish of one layer of vaseline, and on the fabric
Graininess acarid feed is placed, the area that graininess acarid feed is distributed on the fabric at this time is test initial area；
S20, the acarid that predetermined amount is put into the first culture dish, after cultivating preset duration, graininess acarid feed at this time
The area being distributed on the fabric is product behind test cultures；
The difference of product and test initial area is test diffusion area behind S30, test cultures, passes through range estimation or apparatus measures
Obtain control diffusion area；By the anti-mite effect of the size evaluation fabric of test diffusion area, test diffusion area is smaller, described
The anti-mite effect of fabric is better；If testing diffusion area is equal to zero, the fabric has strong anti-mite effect.
Further, fabric to be tested described in the step S10 is the fabric without anti-mite function, and fabric is through containing
The aqueous solution of detergent to be tested is washed and is rinsed with clear water；In step S30, then it can be evaluated by the size of test diffusion area
The anti-mite effect of detergent, test diffusion area is smaller, and the anti-mite effect of the detergent is better；If test diffusion area is equal to
Zero, the detergent has strong anti-mite effect.
Further, the anti-mite effect testing method, further includes:
In step S10, the control fabric as negative control is placed in the first culture dish, and is put in control fabric
Graininess acarid feed is set, the area that graininess acarid feed is distributed in control fabric at this time is control initial area；It is described
Control fabric refer to fabric without anti-mite effect or only washed with clear water after fabric or be not added with mite-proof agent detergent
Aqueous solution wash and the fabric after being rinsed with clear water.
In step S20, after cultivating preset duration, the area that graininess acarid feed is distributed in control fabric at this time is pair
It is long-pending below according to culture；
S30, control culture is long-pending below and compares the difference of initial area as control diffusion area, passes through range estimation or apparatus measures
Obtain control diffusion area；If testing diffusion area is greater than or equal to control diffusion area, the fabric is without anti-mite effect；If surveying
It tries diffusion area and is less than control diffusion area, the fabric has anti-mite effect；If testing diffusion area is equal to zero, the fabric
With strong anti-mite effect.
Further, fabric to be tested described in the step S10 is the fabric without anti-mite function, and fabric is through containing
The aqueous solution of detergent to be tested is washed and is rinsed with clear water；In step S30, then the anti-mite effect of detergent can be evaluated, if
It tests diffusion area and is greater than or equal to control diffusion area, the detergent is without anti-mite effect；If test diffusion area be less than pair
According to diffusion area, the detergent has anti-mite effect；If testing diffusion area is equal to zero, the detergent has strong anti-mite effect
Fruit.
It further, further include calculating anti-mite value P in step S30, anti-mite value P=control diffusion area/test diffusion
Area；Without anti-mite effect if P < 1.5, anti-mite grade is 1 grade；There is weak anti-mite effect if 1.5≤P < 2, anti-mite grade is
2 grades；There is strong anti-mite effect if P >=2, anti-mite grade is 3 grades.
Preferably, above-mentioned test method, in step S10, first by fabric and control fabric to be tested (if there is control fabric
When) be individually placed in the second different culture dishes, the then placement respectively on fabric and control fabric to be tested
Granular acarid feed and the area that observation graininess acarid feed is distributed on fabric and control fabric to be tested respectively, then
Second culture dish is placed in the first culture dish.
Preferably, graininess acarid feed is placed on to the center of the second culture dish.
Preferably, the second culture dish is symmetrical in the first culture dish, and the center of the first culture dish is symmetrical centre.
It is furthermore preferred that placing the second culture dish of fabric to be tested and placing the second culture dish quantity phase of control fabric
Deng, alternately place, and with the first culture dish center for symmetrical centre, and the center of the first culture dish to it is all second culture
The line distance at the center of ware is consistent.
Preferably, in step S20, acarid is placed on to the center of the first culture dish.
Preferably, in step S20, no less than 500 acarids are put into the first culture dish.
Preferably, in step S20, it is 25 ± 1 DEG C that the first culture dish, which is placed in temperature, the training that relative humidity is 80 ± 5 DEG C
It supports in case, is cultivated 1-3 days under conditions of no light.
Preferably, the fabric is fiber cloth.
It is furthermore preferred that the preferred black of the color of the fabric or blue or other dark colors.
Preferably, the shape of the fabric and area are identical as the shape of the bottom of the second culture dish and floor space.
Preferably, second culture dish is the round culture dish that diameter is 4-10cm.
Preferably, the diameter of first culture dish is greater than 2 times of the diameter of the second culture dish, and first culture dish
Diameter be 18-30cm.
Preferably, the partial size of the graininess acarid feed is less than 60 mesh.
Preferably, the graininess acarid feed is selected from rats and mice experimental animal feed, plant amylum, milk powder, Icing Sugar, ferment
At least one of female powder.
Preferably, the acarid is dust mite or dermatophagoides pteronyssinus.
Compared with prior art, the beneficial effects of the present invention are:
Because published be added in detergent with mite killing or the active compound of drive mite may not be able to be generated reality
Effect be not easy to observe and due to the individual very little of acarid, how anti-mite effect is that exploitation is anti-mite desired by effective evaluation
The premise of function detergent, and insoluble technical problem in the industry.The present invention is by comparing culture front and back graininess acarid
The diffusion of feed distribution area carrys out the anti-mite effect of qualitative and/or quantitative assessment fabric or detergent, easily operated, experiment
Period is short, does not need to carry out many and diverse artificial mite number statistics living, rapidly and efficiently, and it is reproducible.
Figure of description
Fig. 1 is the photo of each culture dish situation when cultivating 2 days during the anti-mite measure of merit of embodiment 1 is tested；
Fig. 2 is the photo of each culture dish situation when cultivating 2 days during the anti-mite measure of merit of embodiment 2 is tested；
Fig. 3 is the photo of each culture dish situation when cultivating 2 days during the anti-mite measure of merit of embodiment 3 is tested；
Fig. 4 is the photo of each culture dish situation when cultivating 2 days during the anti-mite measure of merit of embodiment 4 is tested；
Fig. 5 is the photo of each culture dish situation when cultivating 2 days during the anti-mite measure of merit of embodiment 5 is tested.
Specific embodiment
In order to more fully understand technology contents of the invention, combined with specific embodiments below to technical solution of the present invention
It is described further and illustrates, but the present invention is not limited to specific embodiments below.
Fabric used is general fibre cloth, i.e. the non-functional fiber cloth without anti-mite function in the embodiment of the present invention.
Term " detergent " of the invention refers to routinely detergent technique production, using surfactant as main component
Detergent；Term " standard detergent " refers to the detergent without the mite-proof agent artificially added.
Sample in the embodiment of the present invention for test includes test target detergent sample and standard detergent sample, is divided
It is not as follows:
Sample 1: standard detergent: 3%C10-C13 sodium n-alkylbenzenesulfonate, 6% ethoxylated fatty alcohol sulfate,
8% ethoxylized fatty alcohol, 1% sodium citrate, 0.8% alkali protease, 0.05% methylisothiazolinone, < 1% essence,
Surplus is water.
Sample 2: low concentration mite-proof agent X detergent: 3%C10-C13 sodium n-alkylbenzenesulfonate, 6% ethoxylated fat
Alcohol sulfate, 8% ethoxylized fatty alcohol, 0.05% geraniol, 1% sodium citrate, 0.8% alkali protease, 0.05% first
Base isothiazolinone, < 1% essence, surplus are water.
Sample 3: high concentration mite-proof agent X detergent: 3%C10-C13 sodium n-alkylbenzenesulfonate, 6% ethoxylated fat
Alcohol sulfate, 8% ethoxylized fatty alcohol, 5% geraniol, 1% sodium citrate, 0.8% alkali protease, 0.05% methyl are different
Thiazolinone, < 1% essence, surplus are water.
Sample 4: target detergent: 3%C10-C13 sodium n-alkylbenzenesulfonate, 6% ethoxylated fatty alcohol sulfate,
8% ethoxylized fatty alcohol, 1% Ergol, 0.5% dihydrojasmonate, 1% sodium citrate, 0.8% alkalinity
Protease, 0.05% methylisothiazolinone, < 1% essence, surplus are water.
Test target detergent sample and standard detergent sample or clear water are directly compared test by the present invention, are used
The evaluation criterion of the following table 1 qualitatively judges the grade of the anti-mite effect of test target detergent sample, or using the following table 2
Evaluation criterion quantitatively judges the grade of the anti-mite effect of test target detergent sample.
The anti-mite effect qualitative evaluation standard of 1 detergent sample of table
The anti-mite effect quantitatively evaluation criterion of 2 detergent sample of table
The calculation formula of anti-mite value P are as follows:
In formula:
SC1: after default culture duration (usually for 24 hours, 48h, 72h), compare graininess acarid feed distribution in culture dish
The sum of area on control fiber cloth, i.e. control culture are long-pending below；
SC0: when 0h, compares graininess acarid feed in culture dish and be distributed in the sum of the area on control fiber cloth, that is, compare
Initial area；
St1: after default culture duration (usually for 24 hours, 48h, 72h), graininess acarid feed is distributed in test cultures ware
The sum of area in test fiber cloth, i.e., product behind test cultures；
St0: when 0h, graininess acarid feed is distributed in the sum of the area in test fiber cloth in test cultures ware, that is, tests
Initial area.
In triplicate, anti-mite value P takes the average value of retest three times for the test of each sample.
Embodiment 1
The anti-mite effect of standard detergent sample is observed using clear water as negative control
1, sample cloth is handled
Prepare the round culture dish A that 4 diameters are 5.5cm, black fiber cloth is cut into the circle that 4 pieces of diameters are 5.5cm
Shape fiber cloth A.The concentration for preparing 1L standard detergent (sample 1) is the water-detergent solution of 1wt.%, pours into simulation washer
In (salad spinner), after two pieces of fiber cloth A are washed 10min in simulation washer, rinsed 2 times, each 5min with clear water,
It dries, is respectively put into 2 round culture dish A, as test cultures ware A after rinsingt；By other two pieces of fiber cloths A clear water
It dries, is respectively put into other 2 round culture dish A, as control culture dish A after washingc.In test cultures ware AtAnd control
Culture dish AcCenter such as is respectively put at the isometric fine particle shape acarid feed (being put into 0.05g plant amylum) of weight.
2, experiment test and culture
Prepare the round culture dish B that a diameter is 20cm, by 2 test cultures ware AtWith 2 control culture dish AcAlternately
It is spaced apart in round culture dish B, keeps the line distance at the center round culture dish B to all circle centers culture dish A consistent,
It is centrosymmetric with the center of round culture dish B.
Vaseline is applied in the inner wall of round culture dish B, and is put into no less than 500 dust mites at its center, then will be justified
Shape culture dish B is placed in 28 DEG C, in the incubator under the conditions of relative humidity 80%, and no light culture 2 days.
3, experimental result
The result of culture 2 days is as shown in Figure 1, " standard detergent " refers to test cultures ware A in Fig. 1t, " clear water " refers to control training
Support ware Ac.It can be seen that the area that scatters of graininess acarid feed on standard detergent sample cloth greater than clear water from the result of Fig. 1
Compare the area that scatters of the graininess acarid feed on cloth, that is, test diffusion area is greater than control diffusion area, the present embodiment
In for test standard detergent without anti-mite effect.
Embodiment 2
Observe the anti-mite effect of the detergent of the X of mite-proof agent containing low concentration
1, sample cloth is handled
Prepare the round culture dish A that 4 diameters are 5.5cm, black fiber cloth is cut into the circle that 4 pieces of diameters are 5.5cm
Shape fiber cloth A.The concentration for preparing 1L low concentration mite-proof agent X detergent (sample 2) is 1wt.% water-detergent solution, pours into simulation
In washer (salad spinner), by two pieces of fiber cloth A simulation washer in wash 10min after, with clear water rinse 2 times, often
Secondary 5min dries after rinsing, is respectively put into 2 round culture dish A, as test cultures ware At；By other two pieces of fiber cloth A
After washing 10min with the water-detergent solution that the concentration of 1L standard detergent (sample 1) is 1wt.%, rinsed 2 times with clear water,
It dries, is respectively put into other 2 round culture dish A, as control culture dish Ac.In test cultures ware AtWith control culture dish
AcCenter such as is respectively put at the isometric fine particle shape acarid feed (being put into 0.05g plant amylum) of weight.
2, experiment test and culture
Prepare the round culture dish B that 1 diameter is 20cm, by 2 test cultures ware AtWith 2 control culture dish AcAlternately
It is spaced apart in round culture dish B, keeps the line distance at the center round culture dish B to all circle centers culture dish A consistent,
It is centrosymmetric with the center of round culture dish B.
Vaseline is applied in the inner wall of round culture dish B, and is respectively put into no less than 500 powder in the center round culture dish B
Then round culture dish B is placed in 28 DEG C, in the incubator under the conditions of relative humidity 80% by dust mite, and no light culture 2 days.
3, experimental result
The result of culture 2 days is as shown in Fig. 2, " A1 " refers to test cultures ware A in Fig. 2t, " C1 " refers to control culture dish Ac.From figure
In can be seen that AtThe area that scatters of middle graininess acarid feed is greater than AcThe area that scatters of middle graininess acarid feed, i.e. test expand
It dissipates area and is greater than control diffusion area, the detergent (sample 2) of test is used in the present embodiment without anti-mite effect, anti-mite grade to be
1。
Embodiment 3
Observe the anti-mite effect of the detergent of the X of mite-proof agent containing high concentration
1, sample cloth is handled
Prepare the round culture dish A that 4 diameters are 5.5cm, black fiber cloth is cut into the circle that 4 pieces of diameters are 5.5cm
Shape fiber cloth A.The concentration for preparing 1L high concentration mite-proof agent X detergent (sample 3) is 1wt.% water-detergent solution, pours into mould
In quasi- washer (salad spinner), after two pieces of fiber cloth A are washed 10min in simulation washer, rinsed 2 times with clear water,
Each 5min dries after rinsing, is respectively put into 2 round culture dish A, as test cultures ware At；By other two blocks of fibers
After cloth A washs 10min with the water-detergent solution that the concentration of 1L standard detergent (sample 1) is 1wt.%, with clear water rinsing 2
It is secondary, it dries, is respectively put into other 2 round culture dish A, as control culture dish Ac.In test cultures ware AtIt is cultivated with control
Ware AcCenter such as is respectively put at the isometric fine particle shape acarid feed (being put into 0.05g plant amylum) of weight.
2, experiment test and culture
Prepare the round culture dish B that 1 diameter is 20cm, by 2 test cultures ware AtWith 2 control culture dish AcAlternately
It is spaced apart in round culture dish B, keeps the line distance at the center round culture dish B to all circle centers culture dish A consistent,
It is centrosymmetric with the center of round culture dish B.
Vaseline is applied in the inner wall of round culture dish B, and is respectively put into no less than 500 powder in the center round culture dish B
Then round culture dish B is placed in 28 DEG C, in the incubator under the conditions of relative humidity 80% by dust mite, and no light culture 2 days.
3, experimental result
The result of culture 2 days is as shown in figure 3, " A2 " refers to test cultures ware A in Fig. 3t, " C2 " refers to control culture dish Ac.Fig. 3
Show AtMiddle graininess acarid feed scatters in confused situation aobvious, AcMiddle graininess acarid feed scatters completely, i.e. test diffusion area
Greater than control diffusion area, the detergent (sample 3) in the present embodiment for test has strong anti-mite effect, and anti-mite grade is 3.
Can be illustrated by the test result of embodiment 2 and embodiment 3 can be effectively to washing according to test method provided by the invention
The anti-mite effect for washing agent is judged.
Embodiment 4 and embodiment 5
1, sample cloth is handled
Prepare the round culture dish A that 4 diameters are 5.5cm, black fiber cloth is cut into the circle that 4 pieces of diameters are 5.5cm
Shape fiber cloth A.The concentration for preparing 1L test target detergent (sample 4) is the water-detergent solution of 1wt.%, pours into simulation and washes
Wash in device (salad spinner), by two pieces of fiber cloth A simulation washer in wash 10min after, with clear water rinse 2 times, every time
5min dries after rinsing, is respectively put into 2 round culture dish A, as test cultures ware At；Other two pieces of fiber cloth A are used
After the water-detergent solution that the concentration of standard detergent (sample 1) is 1wt.% washs 10min, is rinsed 2 times, is dried with clear water,
It is respectively put into other 2 round culture dish A, as control culture dish Ac.In test cultures ware AtWith control culture dish AcCenter
It the isometric fine particle shape acarid feed (being put into 0.05g plant amylum) of weight such as is respectively put into, is distinguished using measuring tool
The area that metering graininess acarid feed is distributed on fiber cloth A is denoted as test initial area and control initial area respectively.
2, experiment test and culture
Prepare the round culture dish B that a diameter is 20cm, by 2 test cultures ware AtWith 2 control culture dish AcAlternately
Be placed in round culture dish B, keep the line distance at the center round culture dish B to all circle centers culture dish A consistent, i.e., with
The center of round culture dish B is centrosymmetric.
Vaseline is applied in the inner wall of round culture dish B, and is put into no less than 500 dust mites at its center, then will be justified
Shape culture dish B is placed in 28 DEG C, in the incubator under the conditions of relative humidity 80%, and no light culture 2 days.Use measuring tool point
Not Ji Liang the area that is distributed on fiber cloth A of graininess acarid feed, after being denoted as product behind test cultures respectively and compareing culture
Area.
The difference for calculating product and test initial area behind test cultures, is denoted as test diffusion area；After calculating control culture
Area and the difference for compareing initial area, are denoted as control diffusion area.
Experiment is repeated 3 times, and test diffusion area and control diffusion area take being averaged for three repeated experiments resulting value respectively
Value.
3, parallel laboratory test
Repetitive operation above-mentioned steps 1-2 is primary, is embodiment 5.
4, experimental result is evaluated
Fig. 4 be cultivated in embodiment 42 days as a result, Fig. 5 be embodiment 5 cultivate 2 days as a result, " S1 " refers to test in Fig. 4
Culture dish At, " CS1 " refers to control culture dish Ac, " S2 " refers to test cultures ware A in Fig. 5t, " CS2 " refers to control culture dish Ac.It uses
Ocular estimate, AcGraininess acarid feed on middle fiber cloth A scatters completely, AtFeed in middle fiber cloth situation of scattering is similar,
It scatters unobvious, illustrates that test target detergent (sample 4) has strong anti-mite effect, anti-mite grade is 3, twice parallel testing knot
Fruit is consistent.
According to using measuring tool to measure resulting test initial area, control initial area, product behind test cultures, right
It is long-pending below according to culture, it calculates separately to obtain test diffusion area and control diffusion area, then according to the calculation formula of anti-mite value P
Anti-mite value P is calculated, concrete outcome is shown in Table 3.
3 embodiment 4 of table and the anti-mite measure of merit result of the parallel laboratory test twice of embodiment 5
As shown in table 3, twice in parallel laboratory test, 3 times duplicate P value is both greater than 2, and repeatability is good, parallel laboratory test 1
P average value be 2.6, the P average value of parallel laboratory test 2 is 2.5, and the anti-mite grade of parallel laboratory test twice is 3, illustrates the sample
There is strong anti-mite effect, repeatability is good for parallel laboratory test twice.Range estimation and measurement method show replicate test result one twice
It causes, illustrates that the repeatability of this method and confidence level are very high.
It is described above that technology contents of the invention are only further illustrated with embodiment, in order to which reader is easier to understand,
But embodiments of the present invention are not represented and are only limitted to this, any technology done according to the present invention extends or recreation, is sent out by this
Bright protection.
Claims (10)
1. a kind of anti-mite effect testing method, comprising the following steps:
S10, fabric to be tested is placed in inner wall coated in the first culture dish of one layer of vaseline, and placed on the fabric
Graininess acarid feed, the area that graininess acarid feed is distributed on the fabric at this time are test initial area；
S20, the acarid that predetermined amount is put into the first culture dish, after cultivating preset duration, graininess acarid feed is being knitted at this time
The area being distributed on object is product behind test cultures；
The difference of product and test initial area is test diffusion area behind S30, test cultures, is obtained by range estimation or apparatus measures
Diffusion area is tested, by the anti-mite effect of the size qualitative evaluation fabric of test diffusion area, test diffusion area is smaller, described
The anti-mite effect of fabric is better；If testing diffusion area is equal to zero, the fabric has strong anti-mite effect.
2. anti-mite effect testing method according to claim 1, which is characterized in that the anti-mite effect testing method, also
Include:
In step S10, the control fabric as negative control is placed in the first culture dish, and the placement in control fabric
Granular acarid feed, the area that graininess acarid feed is distributed in control fabric at this time are control initial area；The control
Fabric refer to fabric without anti-mite effect or only washed with clear water after fabric or water through being not added with mite-proof agent detergent
Solution washs and the fabric after being rinsed with clear water.
In step S20, after cultivating preset duration, the area that graininess acarid feed is distributed in control fabric at this time is control training
Support product below；
In step S30, control culture is long-pending below and compares the difference of initial area as control diffusion area, is surveyed by range estimation or instrument
Amount obtains control diffusion area；If testing diffusion area is greater than or equal to control diffusion area, the fabric is without anti-mite effect；If
It tests diffusion area and is less than control diffusion area, the fabric has anti-mite effect；If test diffusion area is equal to zero, described to knit
Object has strong anti-mite effect.
3. anti-mite effect testing method according to claim 2, which is characterized in that further include that calculating is anti-mite in step S30
Value P, anti-mite value P=compare diffusion area/test diffusion area；For fabric without anti-mite effect, anti-mite grade is 1 if P < 1.5
Grade；Fabric has weak anti-mite effect if 1.5≤P < 2, and anti-mite grade is 2 grades；Fabric has strong anti-mite effect if P >=2, prevents
Mite grade is 3 grades.
4. anti-mite effect testing method according to claim 1-3, which is characterized in that described in step S10 to
The fabric of test is the fabric without anti-mite function, and the fabric is washed through the aqueous solution containing detergent to be tested and used clear
Water rinsing；Then in step S30, the evaluation conclusion of the evaluation anti-mite effect of fabric is suitable for evaluating the anti-mite effect of the detergent.
5. anti-mite effect testing method according to claim 1-3, which is characterized in that in step S20, by first
It is 25 ± 1 DEG C that culture dish, which is placed in temperature, in the incubator that relative humidity is 80 ± 5 DEG C, cultivates 1-3 under conditions of no light
It.
6. anti-mite effect testing method according to claim 1-3, in step S10, first by fabric to be tested
It is placed in the second culture dish, graininess acarid feed is then placed on fabric to be tested and observes graininess acarid feed
Second culture dish is then placed in the first culture dish by the area being distributed on fabric to be tested；
If when including control fabric, control fabric being placed in the second different culture dishes, then being placed in control fabric
Graininess acarid feed simultaneously observes the area that graininess acarid feed is distributed in control fabric, then puts second culture dish
It is placed in the first culture dish.
7. anti-mite effect testing method according to claim 6, the second culture dish is symmetrical in the first culture dish, with
The center of first culture dish is symmetrical centre；If when including control fabric, place control fabric the second culture dish and place to
Second culture dish quantity of the fabric of test is equal, and alternately places, and with the center of the first culture dish for symmetrical centre, and
The line distance at the first culture dish center to all second culture dishes center is consistent；In step S20, acarid is placed on the first culture
The center of ware.
8. anti-mite effect testing method according to claim 7, which is characterized in that in step S20, into the first culture dish
It is put into no less than 500 acarids.
9. anti-mite effect testing method according to claim 1-3, which is characterized in that the graininess acarid is raised
The partial size of material is less than 60 mesh.
10. anti-mite effect testing method according to claim 1-3, which is characterized in that the acarid is dust
Mite or dermatophagoides pteronyssinus.
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