CN109187696A - The acquisition of electrochemical sensor and density of hydrogen and the foundation of hydrogen treating mode - Google Patents
The acquisition of electrochemical sensor and density of hydrogen and the foundation of hydrogen treating mode Download PDFInfo
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- CN109187696A CN109187696A CN201810884819.7A CN201810884819A CN109187696A CN 109187696 A CN109187696 A CN 109187696A CN 201810884819 A CN201810884819 A CN 201810884819A CN 109187696 A CN109187696 A CN 109187696A
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Abstract
The preparation method for the density of hydrogen in electrochemical sensor and alimentary canal that the invention discloses a kind of for measuring the density of hydrogen in alimentary canal and the method for building up of hydrogen treating mode, comprising: shell;As the hydrogen in working electrode and alimentary canal oxidation reaction occurs for first electrode unit;Second electrode unit, as auxiliary electrode and the polarization circuit of first electrode unit group;Third electrode unit, as reference electrode and first electrode unit composition measurement control loop, with keep first electrode unit current potential it is constant;Wherein, first electrode unit, second electrode unit, third electrode unit main body be set in the housing.This kind is used to measure the electrochemical sensor of the density of hydrogen in alimentary canal, can be avoided the interference that external factor measures density of hydrogen, and the monitoring efficiency of density of hydrogen is higher.
Description
Technical field
The invention belongs to the design fields of Animal diseases treatment ancillary equipment, more particularly to one kind is for measuring in alimentary canal
The electrochemical sensor of density of hydrogen and the preparation method of the density of hydrogen in alimentary canal and hydrogen treating mode foundation side
Method.
Background technique
Hydrogen has permeability strong as a kind of very potential curative medical gas, safe and efficient, selectivity
It removes peroxide, influence the advantages of body antioxidant system itself, more and more zooperies have confirmed, hydrogen pair
All kinds of acute and chronic diseases all have good therapeutic value.
And the monitoring of the existing concentration to alimentary canal hydrogen is mostly using expiration measuring method, Nielsn describes human body within 1961 years
The relationship that hydrogen content and enteron aisle hydrogen generate in expiratory air, and establish measuring method --- the hydrogen of trace hydrogen in expiration
Breathing test.The step of existing method: the method for breath hydrogen test (BHT) is after measuring breath hydrogen on an empty stomach in the morning, to by
Examination person's oral test sugar, periodically collects terminal tidal air, hydrogen and carbon dioxide level in each sample is measured, with minimum breath hydrogen
Concentration is as baseline, and on an empty stomach, usually (10ppm), expiration hydrogen concentration is more than baseline or more prompt test sugar to hydrogen concentration in alveolar
It is fermented by intestinal bacteria.Expiration hydrogen content is in the generation of hydrogen, disperse, excretion and utilizes etc. on links by many factors shadow
It rings, the BHT testing time often needs 3,4 hours, easily occurs some parenteral influence factors in test, so should sentence using when BHT
Surely the standard state tested, the drug that should be disabled before clear stipulaties test, food and empty stomach time.
Since existing expiration measuring method is disturbed, factor is more, lacks the density of hydrogen in effective detection alimentary canal
Monitoring means.
Summary of the invention
The electrochemical sensor and digestion that the object of the present invention is to provide a kind of for measuring the density of hydrogen in alimentary canal
The preparation method of density of hydrogen in road and the method for building up of hydrogen treating mode, can be avoided external factor to density of hydrogen
The interference of measurement, and the monitoring efficiency of density of hydrogen is higher.
To solve the above problems, the technical solution of the present invention is as follows:
It is a kind of for measuring the electrochemical sensor of the density of hydrogen in alimentary canal, comprising:
Shell;
As the hydrogen in working electrode and the alimentary canal oxidation reaction occurs for first electrode unit;
Second electrode unit, as auxiliary electrode and the polarization circuit of first electrode unit group;
Third electrode unit, as reference electrode and first electrode unit composition measurement control loop, to keep
State the constant of the current potential of first electrode unit;
Wherein,
The first electrode unit, second electrode unit, third electrode unit main body be disposed in the housing.
An embodiment according to the present invention, the first electrode unit, second electrode unit, third electrode unit electrode tip
It is integrated to form hydrogen test section.
The size of an embodiment according to the present invention, the electrode tip of the first electrode unit is no more than 0.3mm;Described second
The size of the electrode tip of electrode unit is no more than 0.3mm;The size of the electrode tip of the first electrode unit is no more than 2mm.
An embodiment according to the present invention, the first electrode unit, second electrode unit, third electrode unit electrode tip
It is integrated to form hydrogen test section;The size span of the detection sectional plane of the hydrogen test section is no more than 2.6mm.
An embodiment according to the present invention, the first electrode unit and/or the second electrode unit are glass-carbon electrode, platinum
Electrode, gold electrode, silver electrode, any one in lead electrode.
An embodiment according to the present invention, the third electrode are silver/silver chloride electrode;
Silver/the silver chloride electrode includes:
Surface is covered with the metallic silver of silver chloride layer;
Saturated potassium chloride solution;And
For storing the capsule of the saturated potassium chloride solution;
Wherein,
The first end of the capsule is the electrode tip of the silver/silver chloride electrode, and the end face of the first end of the capsule is
Porous septum;
The metallic silver that the surface is covered with silver chloride layer is placed in the saturated potassium chloride solution, and part stretches out in
The second end of the capsule.
An embodiment according to the present invention, the porous septum are porous ceramics.
A kind of preparation method of density of hydrogen in alimentary canal, uses electrochemical sensing described in above-mentioned any one embodiment
Device measures the density of hydrogen in alimentary canal;
Wherein, the density of hydrogen and the electric current of electrochemical sensor detection are in a linear relationship.
A kind of method for building up of hydrogen treating mode, the method for building up of the hydrogen treating mode are that hydrogen intervenes animal disease
The method for building up of the mode of sick drug treatment, includes the following steps:
A. the administration model of the Animal diseases treatment is established;
B. the administration time point in the administration model feeds hydrogen to the alimentary canal of the animal;
C. detection time length Δ T is set;
D. after to the alimentary canal of animal feeding hydrogen Δ T time length, the hydrogen in above-mentioned alimentary canal is used
The preparation method of gas concentration obtains the gastral density of hydrogen of the animal;
E. the morbid state of the feeding hydrogen front and back animal, influence of the analysis feeding hydrogen to the Animal diseases are compared;
F. the result in conjunction with step d and step e establishes the mode that the hydrogen intervenes Animal diseases drug treatment.
The present invention due to using the technology described above, makes it have the following advantages that and actively imitate compared with prior art
Fruit:
1) electrochemical sensor for measuring the density of hydrogen in alimentary canal that one embodiment of the invention provides can be straight
Connect within alimentary canal hydrogen carry out measurement of concetration, relative to existing expiration measuring method, avoid except alimentary canal because
Influence of the element to density of hydrogen, measurement result are more accurate;In addition, being measured using electrochemical sensor can be quickly obtained
Measurement result, thus it is also higher to the monitoring efficiency of density of hydrogen.
2) by integrating and to be formed the electrode tip of first electrode unit, second electrode unit, third electrode unit
Hydrogen test section, researcher more convenient in this way carry out density of hydrogen monitoring to alimentary canal.
3) preparation method of the density of hydrogen in a kind of alimentary canal that one embodiment of the invention provides is by using the present invention
Electrochemical sensor, the electric current detected using density of hydrogen and electrochemical sensor is in a linear relationship have been obtained in alimentary canal
Density of hydrogen can facilitate researcher to study influence of the density of hydrogen to animal pathological characters in animal alimentary canal.
4) method for building up for a kind of hydrogen treating mode that one embodiment of the invention provides is a kind of hydrogen intervention animal
The method for building up of the mode of disease drug treatment, by establishing the administration model of Animal diseases treatment, in corresponding administration time
Point feeds hydrogen to the alimentary canal of animal, passes through the front and back morbid state comparison to animal after a period of time and animal after a period of time
Density of hydrogen in alimentary canal can analyze influence of the feeding hydrogen to Animal diseases, and further establish hydrogen and intervene animal
The mode of disease drug treatment.The mode for intervening Animal diseases drug treatment by establishing hydrogen, can reasonably use hydrogen
Assist in the treatment of the disease of animal.
Detailed description of the invention
Fig. 1 is the configuration diagram of hydrogen gas electrochemical sensor of the invention;
Fig. 2 is a kind of flow chart of the method for building up of hydrogen treating mode of the invention.
Description of symbols:
1: first electrode unit;2: second electrode unit;3: third electrode unit;4: saturated potassium chloride solution;5: capsule;
6: porous septum.
Specific embodiment
Below in conjunction with the drawings and specific embodiments to proposed by the present invention a kind of for measuring the density of hydrogen in alimentary canal
Electrochemical sensor and alimentary canal in the preparation method of density of hydrogen and the method for building up of hydrogen treating mode make it is further
It is described in detail.According to following explanation and claims, advantages and features of the invention will be become apparent from.
It is a kind of for measuring the electrochemical sensor of the density of hydrogen in alimentary canal, comprising: shell referring to Fig. 1;First electricity
As the hydrogen in working electrode and alimentary canal oxidation reaction occurs for pole unit 1;Second electrode unit 2, as auxiliary electrode and
1 group of polarization circuit of first electrode unit;Third electrode unit 3, as reference electrode and the composition measurement control of first electrode unit 1
Circuit processed, with keep first electrode unit 1 current potential it is constant;Wherein, first electrode unit 1, second electrode unit 2, third
The main body of electrode unit 3 is set in the housing.
It is recognised that electrochemical sensor to tested gas by reacting and generating the electricity directlyed proportional to gas concentration
Signal carrys out work.Electrochemical sensor provided in this embodiment for measuring the density of hydrogen in alimentary canal can be offseted directly
Change the hydrogen progress measurement of concetration within road and avoids the factor except alimentary canal to hydrogen relative to existing expiration measuring method
The influence of gas concentration, measurement result are more accurate;In addition, measurement knot can be quickly obtained by being measured using electrochemical sensor
Fruit, thus it is also higher to the monitoring efficiency of density of hydrogen.It in other words, can be direct using the electrochemical sensor in the present embodiment
The density of hydrogen of alimentary canal privileged site is detected, " loss " will not occur in measurement process for the hydrogen in alimentary canal.
In a specific embodiment, the electrode of first electrode unit 1, second electrode unit 2, third electrode unit 3
It holds integrated to form hydrogen test section.It is appreciated that by the way that first electrode unit 1, second electrode unit 2, third is electric
The electrode tip of pole unit 3 integrates to form hydrogen test section, and researcher more convenient in this way carries out hydrogen to alimentary canal
Concentration monitor.
In particular it is required that the animal of monitoring is mouse, the small intestine diameter of mouse is 3mm, at this point, first electrode unit 1
The size of electrode tip is no more than 0.3mm;The size of the electrode tip of second electrode unit 2 is no more than 0.3mm;First electrode unit 1
Electrode tip size be no more than 2mm.The size span of the detection sectional plane of hydrogen test section is no more than 2.6mm.But it should anticipate
Know, above-mentioned size is not that can not change, and needs to be changed according to the gastral size of the animal monitored.
Specifically, first electrode unit 1 and/or second electrode unit 2 are glass-carbon electrode, platinum electrode, gold electrode, silver electricity
Any one in pole, lead electrode.
Specifically, third electrode is silver/silver chloride electrode;Silver/silver chloride electrode includes: that surface is covered with silver chloride layer
Metallic silver;
Saturated potassium chloride solution 4;And the capsule 5 for storing saturated potassium chloride solution 4;Wherein, the first end of capsule 5
For silver/silver chloride electrode electrode tip, the end face of the first end of capsule 5 is porous septum 6;
The metallic silver that surface is covered with silver chloride layer is placed in saturated potassium chloride solution 4, and part stretches out in capsule 5
Second end.Wherein, porous septum 6 is porous ceramics, for example, zirconia ceramic.Certainly, third electrode can also have other reality
Apply mode, for example, calomel electrode, Ag/AgCl electrode, copper/copper sulfate electrode, high-purity zinc electrode etc..
Based on inventive concept identical with above-described embodiment, the present invention also provides the density of hydrogen in a kind of alimentary canal
Preparation method measures the density of hydrogen in alimentary canal using the electrochemical sensor of above-described embodiment;Wherein, hydrogen is dense
Degree and the electric current of electrochemical sensor detection are in a linear relationship.Pass through the electric current detected using density of hydrogen and electrochemical sensor
The density of hydrogen in a linear relationship obtained in alimentary canal can facilitate researcher to study the density of hydrogen in animal alimentary canal
Influence to animal pathological characters.
Referring to Fig. 2, it is based on inventive concept identical with above-described embodiment, the present invention also provides a kind of hydrogen treating modes
Method for building up, the method for building up of the hydrogen treating mode is the foundation side for the mode that hydrogen intervenes Animal diseases drug treatment
Method includes the following steps:
A. the administration model of Animal diseases treatment is established;Such as disease can be necrotizing enterocolitis
(Necrotizing enterocolitis,NEC);
B. the administration time point in administration model feeds hydrogen to the alimentary canal of animal;
C. detection time length Δ T is set;
D. dense using the hydrogen in above-mentioned alimentary canal after to the alimentary canal of animal feeding hydrogen Δ T time length
The preparation method of degree obtains the gastral density of hydrogen of animal;
E. the morbid state of feeding hydrogen front and back animal, influence of the analysis feeding hydrogen to Animal diseases are compared;
F. the mode that hydrogen intervenes Animal diseases drug treatment is established in conjunction with the result of step d and step e.
It is appreciated that the method for building up of this kind of hydrogen treating mode, is a kind of hydrogen intervention Animal diseases drug treatment
Mode method for building up, by establish Animal diseases treatment administration model, in corresponding administration time point disappearing to animal
Change road and feed hydrogen, passes through the front and back morbid state comparison to animal after a period of time and the hydrogen after a period of time in animal alimentary canal
Gas concentration can analyze influence of the feeding hydrogen to Animal diseases, and further establish hydrogen and intervene Animal diseases drug treatment
Mode.Intervene the mode of Animal diseases drug treatment by establishing hydrogen, reasonably can assist in the treatment of animal using hydrogen
Disease.
Specifically, the hydrogen of animal small intestine position can be tested using the method for electrochemistry, passes through neonate rat
NEC model, the density of hydrogen within different dosing time point, rating model animal alimentary canal tissue can establish hydrogen and digest
Road intracellular metabolite equation.And hydrogen saturation pure water preparation formula cream is attempted, new hydrogen mode of administration is established, for the use of clinical hydrogen
Theoretical foundation is provided, and provides technical guarantee for the metabolism of further genralrlization hydrogen research.
Embodiments of the present invention are explained in detail above in conjunction with attached drawing, but the present invention is not limited to above-mentioned implementations
Mode.Even if to the present invention, various changes can be made, if these variations belong to the model of the claims in the present invention and its equivalent technologies
Within enclosing, then still fall within the protection scope of the present invention.
Claims (9)
1. a kind of for measuring the electrochemical sensor of the density of hydrogen in alimentary canal characterized by comprising
Shell;
As the hydrogen in working electrode and the alimentary canal oxidation reaction occurs for first electrode unit;
Second electrode unit, as auxiliary electrode and the polarization circuit of first electrode unit group;
Third electrode unit, as reference electrode and first electrode unit composition measurement control loop, to keep described the
The current potential of one electrode unit it is constant;
Wherein,
The first electrode unit, second electrode unit, third electrode unit main body be disposed in the housing.
2. as described in claim 1 for measuring the electrochemical sensor of the density of hydrogen in alimentary canal, which is characterized in that institute
It is integrated to form hydrogen test section to state first electrode unit, second electrode unit, the electrode tip of third electrode unit.
3. as described in claim 1 for measuring the electrochemical sensor of the density of hydrogen in alimentary canal, which is characterized in that institute
The size for stating the electrode tip of first electrode unit is no more than 0.3mm;The size of the electrode tip of the second electrode unit is no more than
0.3mm;The size of the electrode tip of the first electrode unit is no more than 2mm.
4. as claimed in claim 3 for measuring the electrochemical sensor of the density of hydrogen in alimentary canal, which is characterized in that institute
It is integrated to form hydrogen test section to state first electrode unit, second electrode unit, the electrode tip of third electrode unit;It is described
The size span of the detection sectional plane of hydrogen test section is no more than 2.6mm.
5. as described in claim 1 for measuring the electrochemical sensor of the density of hydrogen in alimentary canal, which is characterized in that institute
First electrode unit and/or the second electrode unit are stated as in glass-carbon electrode, platinum electrode, gold electrode, silver electrode, lead electrode
Any one.
6. as described in claim 1 for measuring the electrochemical sensor of the density of hydrogen in alimentary canal, which is characterized in that institute
Stating third electrode is silver/silver chloride electrode;
Silver/the silver chloride electrode includes:
Surface is covered with the metallic silver of silver chloride layer;
Saturated potassium chloride solution;And
For storing the capsule of the saturated potassium chloride solution;
Wherein,
The first end of the capsule is the electrode tip of the silver/silver chloride electrode, and the end face of the first end of the capsule is porous
Diaphragm;
The metallic silver that the surface is covered with silver chloride layer is placed in the saturated potassium chloride solution, and is partially stretched out in described
The second end of capsule.
7. as claimed in claim 6 for measuring the electrochemical sensor of the density of hydrogen in alimentary canal, which is characterized in that institute
Stating porous septum is porous ceramics.
8. a kind of preparation method of the density of hydrogen in alimentary canal, which is characterized in that using such as claim 1-7 any one institute
The electrochemical sensor stated measures the density of hydrogen in alimentary canal;
Wherein, the density of hydrogen and the electric current of electrochemical sensor detection are in a linear relationship.
9. a kind of method for building up of hydrogen treating mode, which is characterized in that the method for building up of the hydrogen treating mode is hydrogen
The method for building up for intervening the mode of Animal diseases drug treatment, includes the following steps:
A. the administration model of the Animal diseases treatment is established;
B. the administration time point in the administration model feeds hydrogen to the alimentary canal of the animal;
C. detection time length Δ T is set;
D. after to the alimentary canal of animal feeding hydrogen Δ T time length, using in alimentary canal according to any one of claims 8
The preparation method of density of hydrogen obtain the gastral density of hydrogen of the animal;
E. the morbid state of the feeding hydrogen front and back animal, influence of the analysis feeding hydrogen to the Animal diseases are compared;
F. the result in conjunction with step d and step e establishes the mode that the hydrogen intervenes Animal diseases drug treatment.
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2018
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Application publication date: 20190111 |