CN109187451B - Fluorescent sensor for detecting chemical substance steam - Google Patents
Fluorescent sensor for detecting chemical substance steam Download PDFInfo
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
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- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N21/643—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" non-biological material
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6432—Quenching
Abstract
The present invention provides a fluorescence sensor for detecting a chemical vapor, comprising: the device comprises an excitation light element, a tubular detection cavity, a detection layer coated on the inner wall of the tubular detection cavity and a fluorescence receiving element; the excitation light element is used for irradiating along the axial direction of the tubular detection cavity; the tubular detection cavity is used for coating the detection layer and limiting the gas flow direction of the detected substance; the detection layer reacts with the detected substance to change the intensity or spectrum of the fluorescence excited by the excitation light element; the fluorescence receiving element is used for receiving the light beam affected by the reaction between the detection layer and the detected substance and judging whether the intensity or the spectrum of the fluorescence of the light beam changes. The detection layer of the fluorescence sensor adopts sensitive fluorescent micromolecule nano-material with three-dimensional porous structure to improve the utilization rate of the detected substance and ensure the consistency and reliability of the detection result.
Description
Technical Field
The invention relates to the technical field of substance detection, in particular to a fluorescence sensor.
Background
A fluorescence sensor is a new type of chemical sensor that has been developed in recent years, and the sensor usually contains a sensitive fluorescence sensor material, and interacts with a substance to be detected to change the inherent fluorescence intensity or spectral composition. The fluorescent material has very high sensitivity, and for the detection of a standardized instrument, the sensor designed by the fluorescence principle can be ensured to embody the high sensitivity and the high reliability of the used material only by ensuring that the sensor material is in high-efficiency, uniform and controllable contact with the detected substance.
Most of the existing products adopt a planar fluorescence detection structure, and when the structure is used, the cross section of detected airflow needs to be the same as the macroscopic occupied area of a sensor material. If the cross-sectional area of the airflow is small, part of the material can not contact the gas to be detected, so that the response effect is influenced, and if the cross-sectional area is large, the gas to be detected can be wasted, so that the sensitivity is reduced.
In the plane structure, the air current is perpendicular to the sensor molecules or has a certain angle, which causes the blocking of the air circuit and may cause the local turbulent flow phenomenon, in addition, in the production process of the instrument, the consistency and high collimation of the air circuit are difficult to ensure, therefore, the distribution of the air current of the detection object with different directions and speeds exists, and the probability of actually contacting the sensor molecules is inconsistent on the plane, thereby affecting the sensitivity and reliability of the sensor.
In addition, in the planar detection structure, the areas of the excitation light and the fluorescence receiving element are designed according to the area of the sensor material, so that the universality of the instrument is poor; and the plane type structure does not have air tightness, an additional structural unit is needed to design an air-tight unit along the outer side of the fluorescence receiving plane to complete an air flow loop, the structure is complicated, and the volume of the detection structure is increased.
Disclosure of Invention
The present invention is directed to solving the problems described above. It is an object of the present invention to provide a fluorescence sensor that solves any of the above problems. Specifically, the present invention provides a fluorescent sensor capable of improving the airflow utilization rate and having a compact structure.
To solve the above technical problem, the present invention provides a fluorescence sensor for detecting a chemical vapor, the fluorescence sensor comprising: the device comprises an excitation light element, a tubular detection cavity, a detection layer coated on the inner wall of the tubular detection cavity and a fluorescence receiving element; the excitation light element is positioned at one end of the tubular detection cavity and is used for irradiating along the axial direction of the tubular detection cavity; the tubular detection cavity is used for coating the detection layer and limiting the gas flow direction of the detected substance; the detection layer reacts with the detected substance to change the intensity or spectrum of the fluorescence excited by the excitation light element; the fluorescence receiving element is positioned at the other end of the tubular detection cavity and used for receiving the light beam affected by the reaction between the detection layer and the detected substance and judging whether the intensity or the spectrum of the fluorescence of the light beam changes.
The detection layer is formed by coating sensitive fluorescent micromolecule nano materials.
The thickness of the detection layer is 10-5000 nm, and the coating length in the tubular detection cavity is 2-30 mm.
The thickness of the detection layer is 500-1000 nm, and the coating length in the tubular detection cavity is 10-20 mm.
Wherein, the internal diameter of tubular detection chamber is 0.2 ~ 2mm, and the external diameter is 2 ~ 10mm, and length is 5 ~ 300 mm.
Wherein, the internal diameter of tubular detection chamber is 0.5 ~ 1mm, and the external diameter is 4 ~ 7mm, and length is 15 ~ 50 mm.
The fluorescence sensor also comprises an optical filter, wherein the optical filter is positioned between the fluorescence receiving element and the tubular detection cavity and is used for reducing or eliminating the influence of exciting light on the fluorescence receiving element.
The fluorescence sensor also comprises a diaphragm, wherein the diaphragm is positioned between the excitation light element and the tubular detection cavity and is used for intercepting the excitation light which does not pass through the tubular detection cavity.
The fluorescence sensor also comprises a tubular structural part and a transition pipe, wherein the tubular structural part is hermetically connected with the inlet end of the tubular detection cavity, and the transition pipe is hermetically connected with the outlet end of the tubular detection cavity; the excitation light element is positioned in the tubular structural member; the wall of the transition pipe is provided with a plurality of through holes, so that the steam of the detected substance can be conveniently discharged.
The fluorescence sensor of the invention adopts the tubular detection cavity to improve the sensitivity and reliability of the sensor, and utilizes the parallel of the exciting light element and the irradiation of the tubular detection cavity to further simplify and reduce the product structure; the detection layer adopts sensitive fluorescent micromolecule nano material with three-dimensional porous structure, so that the utilization rate of the detected substance is improved, and the consistency and reliability of the detection result are ensured.
Other characteristic features and advantages of the invention will become apparent from the following description of exemplary embodiments, which is to be read in connection with the accompanying drawings.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of the specification, illustrate embodiments of the invention and together with the description, serve to explain the principles of the invention. In the drawings, like reference numerals are used to indicate like elements. The drawings in the following description are directed to some, but not all embodiments of the invention. For a person skilled in the art, other figures can be derived from these figures without inventive effort.
FIG. 1 schematically shows a structural view of a fluorescence sensor according to the invention;
FIG. 2 schematically illustrates a schematic view of the excitation light component illuminating perpendicular to the tubular detection chamber;
FIG. 3 is a graph illustrating the results of a computational simulation of an excitation light element illuminated perpendicular to a tubular detection chamber;
FIG. 4 schematically illustrates a schematic view of an excitation light element illuminating parallel to a tubular detection chamber;
FIG. 5 is a graph schematically illustrating the results of a computational simulation of an excitation light element illuminated parallel to a tubular detection chamber;
FIG. 6 schematically shows the chemical structure of a sensitive fluorescent small molecule species of the type employed in the detection layer;
FIG. 7 schematically shows the chemical structure of a detection layer material and its synthesis route;
fig. 8 schematically shows a nanostructure of a detection layer.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention. It should be noted that the embodiments and features of the embodiments in the present application may be arbitrarily combined with each other without conflict.
The inventor designs a detection cavity with a tubular structure to simplify the structure of a product, and simultaneously ensures that a detected substance can be fully contacted and acted with a detection layer, so that the sensitivity and the reliability of the sensor are improved; the exciting light element is used for irradiating in parallel to the axial direction of the tubular detection cavity, so that the exciting light is uniformly distributed, and the utilization rate of the material is further improved to improve the consistency of detection results; furthermore, a nano small-molecule fluorescent material with a large specific surface area is coated on the inner surface of the detection cavity to form a detection layer, the influence of the thickness of the detection layer on a detection result is small, and the processing and production of a product are easy.
The fluorescence sensor provided according to the present invention will be described in detail below with reference to the accompanying drawings.
Fig. 1 shows a schematic structural diagram of a specific embodiment of the fluorescence sensor of the present invention, and referring to fig. 1, the fluorescence sensor includes: the device comprises an excitation light element 1, a tubular detection cavity 2, a detection layer 3 coated on the inner wall of the tubular detection cavity 2 and a fluorescence receiving element 4. The fluorescence sensor of the present invention is used for detecting a chemical vapor, and for example, may detect a chemical gas, or may detect a vapor of a substance such as a chemical liquid or a solid.
Wherein, the exciting light component 1 is located at one end of the tubular detection cavity 2 and is used for irradiating along the axial direction of the tubular detection cavity 2 and providing a detected exciting light beam.
The tubular detection cavity 2 is used for coating the detection layer 3 and limiting the gas flow direction of the detected substance; the detected substance enters from one end of the tubular detection cavity 2 under the action of pressure, and the contact time of the detected substance and the detection layer 3 can be accurately controlled by controlling the air flow, so that the detection repeatability of the sensor is ensured.
The detection layer 3 reacts with the detected substance, including physical and/or chemical interaction, to change the intensity or spectrum of the fluorescence excited by the excited light element 1;
the fluorescence receiving element 4 is located at the other end of the tubular detection cavity 2, and is used for receiving the light beam emitted by the excitation light element 1 and affected by the reaction (including the physical and/or chemical interaction) between the detection layer 3 and the detected substance, and determining whether the intensity or spectrum of the fluorescence of the light beam changes.
According to the technical scheme adopted by the invention, the detection cavity is set to be of a tubular structure, and the exciting light element is arranged at one end of the tubular detection cavity and is irradiated along the axial direction of the detection cavity, so that the detection layer is uniformly irradiated, the size of the sensor can be reduced, and the structure is simplified.
Fig. 2 is a schematic view of an irradiation effect of an excitation light element irradiated perpendicular to the axial direction of the tubular detection chamber, fig. 3 is a graph of a calculation simulation result thereof, fig. 4 is a schematic view of an effect of the excitation light element irradiated from one end of the tubular detection chamber along the axial direction thereof, i.e., a schematic view of a use effect of the technical scheme of the present invention, and fig. 5 is a graph of a calculation simulation result of the scheme in fig. 4. As can be seen from comprehensive comparison, when the excitation light element irradiates perpendicularly to the axial direction of the tubular detection cavity, only one side of the tubular detection cavity facing the excitation light element obviously has light beams entering, and the other side of the tubular detection cavity is almost not irradiated by the light beams; however, as shown in fig. 4, when the excitation light element is irradiated from one end of the tubular detection cavity along the axial direction thereof (the light intensity of the light source and the nearest distance from the tubular detection cavity are not changed), the excitation light beam is uniformly distributed along the radial direction of the tubular detection cavity, so that the detection layer is uniformly irradiated and fluoresces, and the detection layer 3 is more fully utilized in the axial direction and the radial direction to verify whether the detected substance can affect the fluorescence of the detection layer.
In addition, the sensor design of the invention has many advantages in terms of the volume of the sensor cavity, and in the sensor structure of the invention, all elements are arranged along the axial direction of the fluorescence detection tube, so that any elements (such as an excitation light element, a fluorescence detection element and the like) are prevented from being arranged in the radial direction, and therefore, the connection or support structure of the elements can be completely eliminated, thereby simplifying the sensor structure; meanwhile, the invention integrates the exciting light element and the fluorescence detection element into the gas path system, compared with the technical scheme of the prior design, the volume of the sensor is further reduced, and the invention is beneficial to developing portable detection equipment such as handheld type and wearable type, and can develop a detection instrument aiming at small holes.
It should be noted that, in the fluorescence sensor of the present invention, the detection layer 3 is formed by coating sensitive fluorescent small molecule nano-material. The sensitive fluorescent small molecule nano material is a three-dimensional porous material, and the specific surface area of the material is larger than that of the traditional high molecular polymer. The inventor verifies through a large number of experiments that the organic micromolecule nano material is used as the detection layer, the detection effect of the organic micromolecule nano material is slightly influenced by the thickness of the detection layer, and the consistency requirement of large-scale production is better met.
In the prior art, a fluorescent sensor mostly adopts a high molecular polymer as a detection layer. The high molecular polymer can form a flat and compact film, the subunits forming the film structure are high molecular chain skeletons, the thickness of the skeletons is only 2-5 nm, and the skeletons have strong interaction, so the microstructure of the film shows aggregation and dispersion of the skeletons, the thickness shows fluctuation of a few nanometers, and the film has very small pores, and a target detection object is difficult to enter the interior of the high molecular film through the tiny pores. However, when excited by excitation light, the inner polymer still emits fluorescence, so the thickness of the polymer film has a great influence on the detection effect, and similar results are confirmed by the prior publications (references J.Am.chem.Soc.,1998,120(21), pp 5321-5322).
For sensitive fluorescent small molecule nanomaterials, small molecules first form nanostructure assemblies, such as nanowires, nanobelts, nanospheres, nanosheets, etc., through strong interactions (e.g., pi-pi interactions, hydrogen bonding interactions, etc.), with smaller dimensions typically tens of nanometers and longer dimensions of several microns or longer. These nanoassemblies are very rigid due to the strong intermolecular interactions. The structures form a low-density three-dimensional porous material through loose weak interaction, so that gas substances can diffuse for a long distance in the thickness direction of a detection layer formed by the sensitive fluorescent small-molecule nano material. Correspondingly, the small molecule nano material has an effective distance of dozens or even hundreds of nanometers in the thickness direction, even if thickness fluctuation of dozens or even hundreds of nanometers exists, the small molecule nano material does not cause obvious fluctuation to a response signal due to the high efficiency of contacting gas molecules. Therefore, by adopting the sensitive fluorescent small molecule nanostructure for detection, the contact between the substance to be detected and the two-dimensional surface of the high molecular polymer can be converted into the three-dimensional contact between the substance to be detected and the sensing material. Compared with the high requirement of the high-molecular polymer on the thickness of the film due to the response consistency, the sensitive fluorescent small-molecular nano material has great improvement and advantages.
Specifically, the detection layer 3 may be made of a specially designed nano material (nano dots, nano wires, nano strips, and secondary assembly structures thereof) composed of sensitive fluorescent small molecules, and the structure of the material is transferred to the position of the detection layer 3 by a specific method for fixing, thereby achieving the detection effect.
For example, when the fluorescence sensor of the present invention is used for detecting molecular vapor of explosives, sensitive small molecules having a chemical structure as shown in fig. 6, wherein n is 1-20, can be selected. Wherein R is1Is a carbon-carbon double bond (-CH ═ CH-, in both R and S configurations) or a carbon-carbon triple bond (-C ≡ C-); r2Is an alkyl structure formed by straight chain or branched chain carbon chains and has a general formula of-CmH2m+1May be the same or different from each other; r3Is flanked by substituted groups (including hydroxy or phenolic-OH, alkoxy-OC)mH2m+1carbonyl-COCmH2m+Ester group-COOCmH2m+1And (ii) a benzene ring, thiophene, furan, pyrrole group, wherein m is 1-20.
The small molecular substance can form a nanowire structure meeting the use requirement through a certain physical and chemical self-assembly process.
Specifically, a chloroform solution of the above molecules is prepared so that its concentration ranges from 0.01 to 1mg/mL (if necessary, allowed to be heated up to 50 ℃); then quickly injecting the solution into methanol with the volume ratio of 1: 1-1: 20, wherein the temperature range of the methanol is 0-50 ℃ according to needs; and standing the mixed solution for one day, and allowing white flocculent precipitate to appear at the bottom of the solution, thus obtaining the prepared sensitive fluorescent micromolecule nanowire. The material is directly absorbed and injected into the tubular detection cavity 2, and the coating preparation of the detection layer 3 can be completed with the assistance of the vibration of a certain frequency. The material is used for generating specific fluorescence quenching response to a wide variety of explosives (such as TNT), thereby realizing fluorescence detection.
The organic silicon thin film has a width of 10-1000 nm and a length of 0.1-500 mu m, can form a loose three-dimensional net structure, and has a very large specific surface area. Meanwhile, when the gas flows on the surface of the material, the gas can permeate into a bulk phase along a three-dimensional network structure, so that the sensitive fluorescent micromolecule nanowire is efficiently utilized. Compared with the tens of nanometers of high molecular polymer materials, the material can be stacked and attached in the tubular detection cavity 2 in a thicker form, so that the sensitivity is improved, and the service life of the sensitive fluorescent material is prolonged.
In one exemplary embodiment, R1is-C ≡ C-, R2is-n-C8H17，R3Selecting from-o-C6H4-COOCH3And n is 2. Fig. 7 shows the chemical structure of the substance in this example and its synthetic route. When the nano-wire is prepared, the material can be obtained and used after 10mL of 0.2mg/mL solution is injected into 100mL of methanol at room temperature and is kept still for one day. Fig. 8 is a nanostructure view of the material obtained in this example.
In addition, the fluorescence sensor of the invention not only responds to explosives, but also can adopt different sensitive fluorescent micromolecule nano materials as detection layers to detect Volatile Organic Compounds (VOC), toxic industrial gas (TIC), oxidizing gas, reducing gas, gaseous active free radicals, air pollution and the like under the condition of keeping other structures unchanged.
Under general conditions, the inner diameter of the tubular detection cavity 2 is 0.2-2 mm, the outer diameter is 2-10 mm, and the length is 5-300 mm. Preferably, the inner diameter of the tubular detection cavity 2 is 0.5-1 mm, the outer diameter is 4-7 mm, and the length is 15-50 mm.
Suitably, the thickness of the detection layer 3 is 10-5000 nm, and the coating length in the tubular detection cavity 2 is 2-30 mm. Preferably, the thickness of the detection layer 3 can be set to be 500-1000 nm, and the coating length in the tubular detection cavity 2 is 10-20 mm. Sensitive fluorescent small molecule nano material is adopted as the detection layer 3, the thickness selection range is wide, the main reason of the selection upper limit is that the material can absorb exciting light strongly, and the excitation amplitude of the internal detection layer is weakened due to the excessively thick material, so that the overall sensitivity is affected, and the material utilization rate is reduced.
In an exemplary embodiment, the fluorescence sensor of the present invention may further include an optical filter 5, where the optical filter 5 is located between the fluorescence receiving element 4 and the tubular detection chamber 2, and is used for filtering the excitation light, filtering out a spectrum that is not affected by the reaction between the detected substance and the detection layer, and reducing or eliminating the influence of the excitation light on the fluorescence receiving element.
Specifically, the fluorescence sensor further comprises a light barrier 8, wherein the light barrier 8 is arranged between the excitation light element 1 and the tubular detection chamber 2 and is used for intercepting the excitation light which does not pass through the tubular detection chamber 2.
As shown in fig. 1, the fluorescence sensor further includes a tubular structure 9 and a transition tube 10, wherein the tubular structure 9 is connected to the inlet end of the tubular detection chamber 2 in an airtight manner, and the transition tube 10 is connected to the outlet end of the tubular detection chamber 2 in an airtight manner and is located between the optical filter 5 and the tubular detection chamber 2.
The excitation light element 1 and the diaphragm 8 are both located within the tubular structure 9. Specifically, the excitation light element 1 and the diaphragm 8 are both suspended within the tubular structure 9. In the present embodiment, the excitation light element 1 is fixed in the tubular structure 9 by the first support structure 6, the light barrier 8 is fixed in the tubular structure 9 by the second support structure 7, and both the excitation light element 1 and the light barrier 8 are located at the radial center of the tubular structure 9.
The wall of the transition tube 10 is provided with a plurality of through holes, for example, the transition tube 10 at least comprises a section of structure with small holes or a mesoporous structure, which is convenient for the gas of the detected substance to be discharged.
Further, a pipe member 11 for fixing the optical filter 5 and the fluorescence receiving element 4 is provided at the outlet end of the tubular detection chamber 2. The pipe fitting 11 is located at the outlet of the transition pipe 10 and is in airtight connection with the transition pipe 10, and therefore light leakage at the gap is avoided, and the detection result is not affected.
The above-described aspects may be implemented individually or in various combinations, and such variations are within the scope of the present invention.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.
Finally, it should be noted that: the above examples are only for illustrating the technical solutions of the present invention, and are not limited thereto. Although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (6)
1. A fluorescence sensor for detecting a chemical vapor, the fluorescence sensor comprising: the device comprises an excitation light element (1), a tubular detection cavity (2), a detection layer (3) coated on the inner wall of the tubular detection cavity (2) and a fluorescence receiving element (4);
the excitation light element (1) is positioned at one end of the tubular detection cavity (2) and is used for irradiating along the axial direction of the tubular detection cavity (2);
the tubular detection cavity (2) is used for coating the detection layer (3) and limiting the gas flow direction of a detected substance;
the detection layer (3) reacts with the detected substance to change the intensity or spectrum of the fluorescence excited by the excitation light element (1);
the fluorescence receiving element (4) is positioned at the other end of the tubular detection cavity (2) and is used for receiving the light beam affected by the reaction between the detection layer (3) and the detected substance and judging whether the intensity or spectrum of the fluorescence of the light beam changes or not;
the detection layer (3) is formed by coating sensitive fluorescent micromolecule nano materials, and the sensitive fluorescent micromolecule nano materials are low-density three-dimensional porous materials formed by loose weak interaction;
the inner diameter of the tubular detection cavity (2) is 0.2-2 mm, the outer diameter is 2-10 mm, and the length is 5-300 mm;
the fluorescence sensor further comprises a tubular structural part (9) and a transition pipe (10), wherein the tubular structural part (9) is connected with the inlet end of the tubular detection cavity (2) in an airtight mode, and the transition pipe (10) is connected with the outlet end of the tubular detection cavity (2) in an airtight mode;
the excitation light element (1) is positioned in the tubular structural part (9);
the wall of the transition pipe (10) is provided with a plurality of through holes, so that the steam of the detected substance can be conveniently discharged;
the outlet end of the tubular detection cavity (2) is further provided with a pipe fitting (11) for fixing the fluorescence receiving element (4), and the pipe fitting (11) is located at the outlet of the transition pipe (10) and is in airtight connection with the transition pipe (10).
2. The fluorescence sensor according to claim 1, wherein the thickness of the detection layer (3) is 10 to 5000nm and the coating length in the tubular detection chamber (2) is 2 to 30 mm.
3. The fluorescence sensor according to claim 1, wherein the thickness of the detection layer (3) is 500 to 1000nm and the coating length in the tubular detection chamber (2) is 10 to 20 mm.
4. The fluorescence sensor according to claim 1, wherein the tubular detection chamber (2) has an inner diameter of 0.5 to 1mm, an outer diameter of 4 to 7mm, and a length of 15 to 50 mm.
5. The fluorescence sensor according to claim 1, further comprising a filter (5), wherein the filter (5) is located between the fluorescence receiving element (4) and the tubular detection chamber (2) for reducing or excluding the effect of excitation light on the fluorescence receiving element (4).
6. The fluorescence sensor according to claim 1, wherein said fluorescence sensor further comprises a light barrier (8), said light barrier (8) being located between said excitation light element (1) and said tubular detection chamber (2) for intercepting excitation light not passing through said tubular detection chamber (2).
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| CN111380847B (en) * | 2020-03-31 | 2023-08-18 | 深圳大学 | Film double-sided fluorescence signal detection device and detection method |
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