CN109187383B - Method for measuring phytic acid content in mixed solution and eliminating interference of tea polyphenol - Google Patents
Method for measuring phytic acid content in mixed solution and eliminating interference of tea polyphenol Download PDFInfo
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
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Abstract
The invention belongs to the technical field of spectral analysis, and particularly relates to a method for eliminating interference of tea polyphenol on determination of phytic acid content in a mixed solution by using a mathematical fitting method. The method for measuring the phytic acid content in the mixed solution and eliminating the interference of tea polyphenol comprises the following steps: preparing phytic acid standard solutions with different concentrations and a series of phytic acid standard solutions containing tea polyphenol with a certain concentration respectively, eliminating the interference of the tea polyphenol on the basis of obtaining phytic acid standard curves with different tea polyphenol concentrations, and determining the concentration of the phytic acid in the mixed solution of the tea polyphenol and the phytic acid. The method solves the problem of interference of tea polyphenol when the concentration of phytic acid in the mixed solution is determined by a ferric trichloride-sulfosalicylic acid color development method, can be applied to determination of each component in the aquatic product composite preservative, has the advantages of simple method and equipment, convenience in operation, easiness in condition control, low operation cost, high measurement accuracy and the like, and is suitable for popularization.
Description
Technical Field
The invention belongs to the technical field of spectral analysis, and particularly relates to a method for measuring the phytic acid content in a mixed solution and eliminating the interference of tea polyphenol.
Background
China has wide regions, various food types and large yield, so the country pays great attention to the quality and freshness preservation of the food. However, food products are susceptible to various microbial infections during processing, storage and consumption, which can lead to spoilage of the food products. At present, food preservation modes can be divided into two types: one is to keep food fresh by adopting ways of air conditioning, low temperature, irradiation and the like, but the ways have large investment, high cost and more required equipment, and are not suitable for keeping food fresh in small batches. The other type is to adopt the preservative to preserve the food, and the preservative plays an important role as an effective auxiliary means for preserving and processing the food. Aquatic products play an important role in food structure, and the characteristics of low fat, low calorie and high protein of the aquatic products are indispensable elements in reasonable dietary structure, so the aquatic products are very popular with consumers. With the continuous improvement of living standard of people, the consumption of aquatic products is increasing day by day, the requirement on the freshness of the aquatic products is higher and higher, and in view of the great changes of the varieties, the prices, the supply and sale systems and the like of the aquatic products, the preservation technology is well worked out, the effective supply is increased, and the improvement of the aquatic product preservation system has very important significance. However, the aquatic products carry a large amount of bacteria, and are easy to cause color change, flavor change and even putrefaction and deterioration in the processes of storage, transportation, processing and sale. Therefore, the preservation of aquatic products has become a focus of attention of researchers at home and abroad. At present, the preservation of aquatic products tends to a comprehensive application method of preservatives from different sources, so as to be beneficial to exerting the synergistic effect of the preservatives, thereby not only enhancing the bacteriostatic effect of the aquatic products, but also improving the application safety of the aquatic products.
Phytic acid (also known as Phytic acid) has a structure of an inositol derivative in which 6 hydroxyl groups of inositol are phosphorylated. It can be used as antioxidant, antistaling agent, water softener, metal anticorrosive agent, etc., and can be widely used in the fields of food, medicine, textile industry, polymer industry, etc. (the food industry is used for keeping fruits, vegetables and aquatic products fresh and protecting color), and has obvious inhibiting effect on gram-negative bacteria. Tea Polyphenols (Tea Polyphenols, abbreviated as TP), also known as Tea tannin and catechin, belong to Polyphenols, and are the general name of a class of polyhydroxylated phenol compounds contained in Tea leaves. The most important component in tea polyphenol is various catechins (cathins) of flavanols, which accounts for 60-80% of the total phenol content and mainly consists of Epicatechin (EC), Epigallocatechin (EGC), epicatechin gallate (ECG) and epigallocatechin gallate (EGCG), wherein the EGCG is the most important component with the highest content. The biological preservative is safe and nontoxic, is a pure natural biological preservative, has good antibacterial and antioxidant effects on aquatic products, and can effectively inhibit the growth of microorganisms, delay fat oxidation and prolong the shelf life of the aquatic products. The phytic acid and the tea polyphenol are food preservatives, and comprehensive use of the phytic acid and the tea polyphenol is beneficial to obtaining cheap and efficient products.
When the fresh-keeping performance of the phytic acid and the tea polyphenol is researched, the accurate determination of the contents of the two components in the mixed substance is the precondition of working development. The phytic acid and the tea polyphenol can be measured by adopting a spectrophotometry after the color development treatment, wherein the dual-wavelength spectrophotometry is widely applied. Researches show that the phytic acid has no influence on the measurement result of the concentration of the tea polyphenol in the mixed solution, the content of the tea polyphenol has influence on molar absorption coefficients of the phytic acid under different wavelengths after color development treatment, and the accuracy of the measurement result of the dual-wavelength spectrophotometry is further seriously influenced. Therefore, it is necessary to find a simple and accurate method for measuring the phytic acid concentration, which can eliminate the interference of tea polyphenol.
Disclosure of Invention
The purpose of the invention is as follows:
the invention aims to simply and accurately determine the concentration of phytic acid in a mixed solution, overcomes the defects of a dual-wavelength spectrophotometry technology, and particularly relates to a method for eliminating the interference of tea polyphenol during the determination of the concentration of the phytic acid by using a mathematical fitting method.
The technical scheme is as follows:
the invention is implemented by the following technical scheme:
a method for measuring the phytic acid content in a mixed solution and eliminating the interference of tea polyphenol comprises the following specific steps:
the method comprises the following steps: preparing standard tea polyphenol solutions with different concentrations; treating the standard solution by ferrous tartrate developing method, measuring absorbance, and drawing a standard curve by taking the concentration of tea polyphenol as abscissa and the absorbance as ordinate; and (3) processing by adopting a mathematical method to obtain a standard curve simulation equation:
Atea polyphenols＝k1cTea polyphenols+b1 (1)
In the formula: a. theTea polyphenolsIs absorbance; c. CTea polyphenolsThe concentration of the tea polyphenol in the solution is mg/mL; k is a radical of1、b1Is a constant;
step two: respectively preparing phytic acid standard solutions with different concentrations and a series of phytic acid standard solutions containing tea polyphenol with certain concentration; processing the standard solution by adopting a ferric trichloride-sulfosalicylic acid color development method, then measuring the absorbance of the standard solution, respectively drawing standard curves of series phytic acid solutions with different tea polyphenol concentrations, and processing the standard curves by adopting a mathematical method to obtain a standard curve simulation equation:
Ageneral assembly＝kcPhytic acid+b (2)
In the formula: a. theGeneral assemblyIs absorbance, cPhytic acidThe concentration of the phytic acid in the solution is mg/mL; k. b is a constant.
Taking the concentration of the tea polyphenol as an abscissa, respectively drawing by taking k and b of each standard curve as an ordinate, and carrying out mathematical simulation on the curves to obtain the relationship between the concentration of the tea polyphenol in the mixed solution and k and b of a simulation equation of the phytic acid standard curve:
k＝a1c3 tea polyphenols+a2c2 Tea polyphenols+a3cTea polyphenols-a4 (3)
b＝r1c2 Tea polyphenols+r2cTea polyphenols+r3 (4)
In the formula: c. CTea polyphenolsThe concentration of the tea polyphenol in the solution is mg/mL; a is1、a2、a3And a4，r1、r2And r3The resulting coefficients were modeled.
Step three: measuring the content of phytic acid in the mixed solution of tea polyphenol and phytic acid, and eliminating the interference of tea polyphenol.
Taking a mixed solution of tea polyphenol with unknown concentration and phytic acid, treating by a ferrous tartrate chromogenic method, and determining the absorbance A of the solutionTea polyphenolsSubstituting into equation (1) to obtain cTea polyphenols(ii) a C is toTea polyphenolsSubstituting into the formula (3) And in the formula (4), obtaining k and b values; treating by adopting a ferric trichloride-sulfosalicylic acid color development method and measuring the absorbance A of the mixed solutionGeneral assemblyA isGeneral assemblyK and b are substituted into the formula (2), and c can be obtained by calculationPhytic acid。
The method for determining the phytic acid content in the mixed solution and eliminating the interference of tea polyphenol has the best detection range that the concentration of the phytic acid is 0-3.0 mg/mL.
The method for determining the phytic acid content in the mixed solution and eliminating the interference of the tea polyphenol has the best detection range that the concentration of the tea polyphenol is 0-0.3 mg/mL.
The advantages and effects are as follows:
(1) the method has convenient operation and lower requirements on used equipment, can reach the experimental conditions in the current common laboratory, can be used for carrying out experiments by common experimenters, does not need special training, has easily controlled operating conditions and low operating cost;
(2) the invention has high measuring accuracy and small error, the recovery rate can reach more than 97 percent, and the prior art can only reach about 90 percent;
(3) the invention can provide theoretical basis for the determination of other multi-component mixed substances;
(4) eliminating interference, namely eliminating the interference of the tea polyphenol during the determination of the phytic acid concentration by utilizing a mathematical fitting method.
Drawings
FIG. 1 is a standard curve of phytic acid when the concentrations of tea polyphenols in a mixed solution are different;
FIG. 2 is the relationship between the concentration of tea polyphenol in the mixed solution and the slope of the phytic acid standard curve;
FIG. 3 is the relationship between the concentration of tea polyphenols in the mixed solution and the intercept of the phytic acid standard curve.
Detailed Description
A method for measuring the phytic acid content in a mixed solution and eliminating the interference of tea polyphenol comprises the following specific steps:
the method comprises the following steps: preparing standard tea polyphenol solutions with different concentrations; treating the standard solution by ferrous tartrate developing method, measuring absorbance, and drawing a standard curve by taking the concentration of tea polyphenol as abscissa and the absorbance as ordinate; and (3) processing by adopting a mathematical method to obtain a standard curve simulation equation:
Atea polyphenols＝k1cTea polyphenols+b1 (1)
In the formula: a. theTea polyphenolsIs absorbance; c. CTea polyphenolsThe concentration of the tea polyphenol in the solution is mg/mL; k is a radical of1、b1Is a constant;
step two: respectively preparing phytic acid standard solutions with different concentrations and a series of phytic acid standard solutions containing tea polyphenol with certain concentration; processing the standard solution by adopting a ferric trichloride-sulfosalicylic acid color development method, then measuring the absorbance of the standard solution, respectively drawing standard curves of series phytic acid solutions with different tea polyphenol concentrations, and processing the standard curves by adopting a mathematical method to obtain a standard curve simulation equation:
Ageneral assembly＝kcPhytic acid+b (2)
In the formula: a. theGeneral assemblyIs absorbance, cPhytic acidThe concentration of the phytic acid in the solution is mg/mL; k. b is a constant.
Taking the concentration of the tea polyphenol as an abscissa, respectively drawing by taking k and b of each standard curve as an ordinate, and carrying out mathematical simulation on the curves to obtain the relationship between the concentration of the tea polyphenol in the mixed solution and k and b of a simulation equation of the phytic acid standard curve:
k＝1.0522c3 tea polyphenols-0.3044c2 Tea polyphenols+0.0938cTea polyphenols-0.3319 (5)
b＝-0.76c2 Tea polyphenols-0.2165cTea polyphenols+1.4612 (6)
In the formula: c. CTea polyphenolsThe concentration of the tea polyphenol in the solution is mg/mL; a is1、a2、a3And a4And r1、r2And r3The resulting coefficients were modeled.
Step three: measuring the content of phytic acid in the mixed solution of tea polyphenol and phytic acid, and eliminating the interference of tea polyphenol.
Taking a mixed solution of tea polyphenol with unknown concentration and phytic acid, treating by ferrous tartrate color development method, and determining absorbance A of the solutionTea polyphenolsSubstituting into equation (1) to obtain cTea polyphenols(ii) a C is toTea polyphenolsSubstituting into formula (3) and formula (4) to obtain k and b values; treating by adopting a ferric trichloride-sulfosalicylic acid color development method and measuring the absorbance A of the mixed solutionGeneral assemblyA isGeneral assemblyK and b are substituted into the formula (2), and c can be obtained by calculationPhytic acid。
The method for determining the phytic acid content in the mixed solution and eliminating the interference of tea polyphenol has the best detection range that the concentration of the phytic acid is 0-3.0 mg/mL.
The method for determining the phytic acid content in the mixed solution and eliminating the interference of the tea polyphenol has the best detection range that the concentration of the tea polyphenol is 0-0.3 mg/mL.
Namely, in the actual detection, if the phytic acid and the tea polyphenol content in the actual detection object are high, the phytic acid and the tea polyphenol can be diluted in proportion and then measured.
The invention is described in detail below with reference to the accompanying drawings:
example 1:
accurately absorbing 100 mu L of phytic acid standard solutions with different concentrations, adding 2mL of ferric trichloride-sulfosalicylic acid reaction solution, standing for 10min, measuring the absorbance of the solution at 490nm after color development is stable, drawing a standard curve by taking the phytic acid concentration as a horizontal coordinate and the absorbance as a vertical coordinate, and performing mathematical treatment to obtain a simulation equation of the standard curve.
And respectively measuring the standard curves of the phytic acid solution when the concentration of the tea polyphenol is 0.1mg/mL, 0.15mg/mL, 0.2mg/mL, 0.25mg/mL or 0.3mg/mL by adopting the ferric trichloride-sulfosalicylic acid color development method, and mathematically processing the curves to obtain a simulation equation of the standard curves. The standard curve is shown in fig. 1, and the simulation equation of the standard curve is shown in table 1.
TABLE 1 Linear regression equation of phytic acid in mixed solution when tea polyphenol concentration is different
The concentration c of tea polyphenols is plotted on the abscissa and the values of k and b are plotted on the ordinate, as shown in fig. 2 and fig. 3. To c-k goLine-third order polynomial simulation, correlation coefficient R20.9938, the simulation equation is: k-1.0522 c3-0.3044c2+0.0938 c-0.3319; performing second-order polynomial simulation on c-b, and obtaining a correlation coefficient R20.9944, the simulation equation is b-0.76 c2-0.2165c+1.4612。
Preparing a mixed solution with the tea polyphenol concentration of 0.140mg/mL and the phytic acid concentration of 0.300mg/mL, and firstly determining the tea polyphenol concentration of 0.141mg/mL by a ferrous tartrate chromogenic method. Substituting c 0.141mg/mL into the above formulas (5) and (6) gives k-0.3218, b 1.4156, i.e. the standard curve equation of phytic acid is aGeneral assembly＝-0.3218cPhytic acid+1.4156. Treating the mixed solution by ferric trichloride-sulfosalicylic acid color development method, and measuring the absorbance A of the solution at 490nmGeneral assembly1.322 by substituting the above equation to obtain cPhytic acidWhen the yield was 0.291mg/mL, the recovery rate was 96.92%.
Example 2:
preparing a mixed solution with the tea polyphenol concentration of 0.140mg/mL and the phytic acid concentration of 1.800mg/mL, and firstly determining the tea polyphenol concentration of 0.142mg/mL by a ferrous tartrate chromogenic method. Substituting c 0.142mg/mL into the formulas (5) and (6) to obtain k-0.3217, b 1.4151, namely the standard curve equation of phytic acid is AGeneral assembly＝-0.3217cPhytic acid+1.4151. Treating the mixed solution by ferric trichloride-sulfosalicylic acid color development method, and measuring the absorbance A of the solution at 490nmGeneral assemblyC is obtained by substituting the above formulaPhytic acidWhen the yield is 1.782mg/mL, the recovery rate is 98.97%.
Example 3:
preparing a mixed solution with the tea polyphenol concentration of 0.160mg/mL and the phytic acid concentration of 2.900mg/mL, and firstly determining the tea polyphenol concentration of 0.162mg/mL by a ferrous tartrate chromogenic method. Substituting c 0.162mg/mL into the formulas (5) and (6) to obtain k-0.3202, b 1.4062, namely the standard curve equation of phytic acid is AGeneral assembly＝-0.3202cPhytic acid+1.4062. Treating the mixed solution by ferric trichloride-sulfosalicylic acid color development method, and measuring the absorbance A of the solution at 490nmGeneral assembly0.497, substituting the above formula to obtain cPhytic acidWhen the yield was 2.839mg/mL, the recovery rate was 97.90%.
Example 4:
preparing a mixed solution with the tea polyphenol concentration of 0.040mg/mL and the phytic acid concentration of 0.400mg/mL, and firstly determining the tea polyphenol concentration of 0.039mg/mL by using a ferrous tartrate chromogenic method. Substituting c 0.039mg/mL into equations (5) and (6) yields k-0.3286, b 1.4516, the standard curve equation for phytic acid is AGeneral assembly＝-0.3286cPhytic acid+1.4516. Treating the mixed solution by ferric trichloride-sulfosalicylic acid color development method, and measuring the absorbance A of the solution at 490nmGeneral assembly1.322 by substituting the above equation to obtain cPhytic acidWhen the yield was 0.394mg/mL, the recovery rate was 98.59%.
Example 5:
preparing a mixed solution with the tea polyphenol concentration of 0.050mg/mL and the phytic acid concentration of 1.600mg/mL, and firstly measuring the tea polyphenol concentration of 0.051mg/mL by a ferrous tartrate chromogenic method. Substituting c 0.051mg/mL into the formulas (5) and (6) to obtain k-0.3278, b 1.4482, namely the standard curve equation of phytic acid is AGeneral assembly＝-0.3278cPhytic acid+1.4482. Treating the mixed solution by ferric trichloride-sulfosalicylic acid color development method, and measuring the absorbance A of the solution at 490nmGeneral assemblyC is obtained by substituting the above formula to 0.912Phytic acidThe yield was 102.24% at 1.636 mg/mL.
Example 6:
preparing a mixed solution with the tea polyphenol concentration of 0.050mg/mL and the phytic acid concentration of 2.800mg/mL, and firstly determining the tea polyphenol concentration of 0.080mg/mL by a ferrous tartrate chromogenic method. Substituting c 0.080mg/mL into equations (5) and (6) yields k-0.3258, b 1.4390, i.e., the standard curve equation for phytic acid is AGeneral assembly＝-0.3258cPhytic acid+1.4390. Treating the mixed solution by ferric trichloride-sulfosalicylic acid color development method, and measuring the absorbance A of the solution at 490nmGeneral assembly0.560, substituting the above equation to obtain cPhytic acidThe yield was 96.36% when 2.698mg/mL was obtained.
Example 7:
preparing a mixed solution with the tea polyphenol concentration of 0.280mg/mL and the phytic acid concentration of 0.500mg/mL, and firstly measuring the tea polyphenol concentration of 0.283mg/mL by a ferrous tartrate chromogenic method. C is equal to 0283mg/mL into equations (5) and (6) gives k-0.3059, b-1.3391, i.e. the standard curve equation for phytic acid is aGeneral assembly＝-0.3059cPhytic acid+1.3391. Treating the mixed solution by ferric trichloride-sulfosalicylic acid color development method, and measuring the absorbance A of the solution at 490nmGeneral assembly1.190, substituting the above formula to obtain cPhytic acidWhen the yield was 0.487mg/mL, the recovery rate was 97.46%.
Example 8:
preparing a mixed solution with the tea polyphenol concentration of 0.280mg/mL and the phytic acid concentration of 1.500mg/mL, and firstly determining the tea polyphenol concentration of 0.285mg/mL by a ferrous tartrate chromogenic method. Substituting c 0.285mg/mL into the formulas (5) and (6) to obtain k-0.3055, b 1.3378, i.e. the standard curve equation of phytic acid is AGeneral assembly＝-0.3055cPhytic acid+1.3378. Treating the mixed solution by ferric trichloride-sulfosalicylic acid color development method, and measuring the absorbance A of the solution at 490nmGeneral assembly0.885, substituting the above equation to obtain cPhytic acidWhen the yield was 1.482mg/mL, the recovery rate was 98.79%.
Example 9:
preparing a mixed solution with the tea polyphenol concentration of 0.290mg/mL and the phytic acid concentration of 2.700mg/mL, and firstly determining the tea polyphenol concentration of 0.291mg/mL by using a ferrous tartrate chromogenic method. Substituting c 0.291mg/mL into the formulas (5) and (6) to obtain k-0.3045 and b 1.3338, namely the standard curve equation of phytic acid is AGeneral assembly＝-0.3045cPhytic acid+1.3338. Treating the mixed solution by ferric trichloride-sulfosalicylic acid color development method, and measuring the absorbance A of the solution at 490nmGeneral assembly0.516, substituting the above equation to obtain cPhytic acidWhen the yield was 2.686mg/mL, the recovery was 99.49%.
The principle is as follows:
the basis of spectrophotometry is the selective absorption of light by substances. Under the selected wavelength, the absorption degree and the concentration of the colored solution to be measured to the light obey the Lambert-beer law within a certain range: a ═ epsilon bc
In the formula: a-absorbance; epsilon-extinction coefficient, L.mol-1cm-1(ii) a b-optical path, cm; c-concentration of light-absorbing component in solution, mol/L.
The principle of the multi-component quantification method is to use the additivity of the absorbance of the multiple components, i.e. the total absorbance of the mixture is equal to the sum of the absorbance of the components in the mixture.
1. Treating the tea polyphenol solution with gradient concentration and the mixed solution of tea polyphenol and phytic acid by ferrous tartrate developing method, measuring the absorbance of the tea polyphenol solution and the mixed solution of tea polyphenol and phytic acid under a certain wavelength, and drawing a standard curve. The phytic acid developed by the ferrous tartrate has no absorbance, namely A is 0, which shows that the phytic acid has no influence on the determination of the tea polyphenol, and the concentration of the tea polyphenol can be determined by adopting a ferrous tartrate developing method in the presence of the phytic acid.
2. Treating the tea polyphenol solution with gradient concentration, the phytic acid solution and the mixed solution of the tea polyphenol and the phytic acid by adopting a ferric trichloride-sulfosalicylic acid color development method, and measuring and drawing a standard curve. It is found that the tea polyphenol treated by the ferric trichloride-sulfosalicylic acid color development method also develops color, and the tea polyphenol has influence on the color development capability of the phytic acid, but not simple absorbance summation.
3. The concentration of tea polyphenol has an influence on the slope and intercept of the phytic acid standard curve. In order to eliminate the interference of the tea polyphenol in the mixed solution on the color development capability of the phytic acid, the mathematical relationship between the tea polyphenol concentration and the slope and intercept of the phytic acid standard curve can be obtained through mathematical fitting, and the concentration of the phytic acid in the mixed solution can be obtained through calculation.
Claims (3)
1. A method for measuring the phytic acid content in a mixed solution and eliminating the interference of tea polyphenol is characterized in that: the method comprises the following specific steps:
the method comprises the following steps: preparing standard tea polyphenol solutions with different concentrations; treating the standard solution by ferrous tartrate developing method, measuring absorbance, and drawing a standard curve by taking the concentration of tea polyphenol as abscissa and the absorbance as ordinate; and (3) processing by adopting a mathematical method to obtain a standard curve simulation equation:
Atea polyphenols＝k1cTea polyphenols+b1 (1)
In the formula: a. theTea polyphenolsIs absorbance; c. CTea polyphenolsThe concentration of the tea polyphenol in the solution is mg/mL; k is a radical of1、b1Is a constant;
step two: respectively preparing phytic acid standard solutions with different concentrations and a series of phytic acid standard solutions containing tea polyphenol with certain concentration; processing the standard solution by adopting a ferric trichloride-sulfosalicylic acid color development method, then measuring the absorbance of the standard solution, respectively drawing standard curves of series phytic acid solutions with different tea polyphenol concentrations, and processing the standard curves by adopting a mathematical method to obtain a standard curve simulation equation:
Ageneral assembly＝kcPhytic acid+b (2)
In the formula: a. theGeneral assemblyIs absorbance, cPhytic acidThe concentration of the phytic acid in the solution is mg/mL; k. b is a constant;
taking the concentration of the tea polyphenol as an abscissa, respectively drawing by taking k and b of each standard curve as an ordinate, and carrying out mathematical simulation on the curves to obtain the relationship between the concentration of the tea polyphenol in the mixed solution and k and b of a simulation equation of the phytic acid standard curve:
k＝a1c3 tea polyphenols+a2c2 Tea polyphenols+a3cTea polyphenols-a4 (3)
b＝r1c2 Tea polyphenols+r2cTea polyphenols+r3 (4)
In the formula: c. CTea polyphenolsThe concentration of the tea polyphenol in the solution is mg/mL; a is1、a2、a3And a4，r1、r2And r3The coefficients obtained for the simulation;
step three: measuring the content of phytic acid in the mixed solution of tea polyphenol and phytic acid, and eliminating the interference of tea polyphenol;
taking a mixed solution of tea polyphenol with unknown concentration and phytic acid, treating by ferrous tartrate color development method, and determining absorbance A of the solutionTea polyphenolsSubstituting into equation (1) to obtain cTea polyphenols(ii) a C is toTea polyphenolsSubstituting into formula (3) and formula (4) to obtain k and b values; treating by adopting a ferric trichloride-sulfosalicylic acid color development method and measuring the absorbance A of the mixed solutionGeneral assemblyA isGeneral assemblyK, b intoIn the formula (2), c is calculatedPhytic acid。
2. The method for determining the phytic acid content in the mixed solution and eliminating the interference of tea polyphenol according to claim 1, wherein the method comprises the following steps: the optimal detection range is that the concentration of the phytic acid is 0-3.0 mg/mL.
3. The method for determining the phytic acid content in the mixed solution and eliminating the interference of tea polyphenol according to claim 1, wherein the method comprises the following steps: the optimal detection range is 0-0.3 mg/mL of the concentration of the tea polyphenol.
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| CN103234934A (en) * | 2013-03-29 | 2013-08-07 | 江汉大学 | Method for determining fat soluble green tea polyphenol content |
| CN104359887A (en) * | 2014-11-25 | 2015-02-18 | 太原师范学院 | Method for determining raw materials of humic acid product by adopting fluorescence method and method for quickly detecting content of humic acid in humic acid product |
| CN106018337A (en) * | 2016-08-04 | 2016-10-12 | 浙江大学 | Method for determination of phytic acid content of cotton seed powder |
| CN106290341A (en) * | 2016-09-14 | 2017-01-04 | 郑州欧柯奇仪器制造有限公司 | Tea polyphenols method for quick in tea beverage |
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Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011077342A1 (en) * | 2009-12-23 | 2011-06-30 | Danisco A/S | Method |
| CN102033011A (en) * | 2010-11-26 | 2011-04-27 | 河北农业大学 | Soil phytic acid extracting solution and application thereof |
| CN103234934A (en) * | 2013-03-29 | 2013-08-07 | 江汉大学 | Method for determining fat soluble green tea polyphenol content |
| CN104359887A (en) * | 2014-11-25 | 2015-02-18 | 太原师范学院 | Method for determining raw materials of humic acid product by adopting fluorescence method and method for quickly detecting content of humic acid in humic acid product |
| CN106018337A (en) * | 2016-08-04 | 2016-10-12 | 浙江大学 | Method for determination of phytic acid content of cotton seed powder |
| CN106290341A (en) * | 2016-09-14 | 2017-01-04 | 郑州欧柯奇仪器制造有限公司 | Tea polyphenols method for quick in tea beverage |
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