CN109182542A - One kind SNP marker relevant to chicken Carcass Traits and its application - Google Patents
One kind SNP marker relevant to chicken Carcass Traits and its application Download PDFInfo
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Abstract
The invention discloses a kind of SNP marker relevant to chicken Carcass Traits and its applications in breeding, its described SNP marker corresponds to the g.526 G > A of the mutational site in chicken let-7b prosoma gene order, and the genotype of the SNP site is AA, GA and GG.SNP marker of the invention, g.526 G > A is related to chicken Carcass Traits in mutational site i.e. in chicken let-7b prosoma gene order, it is a new molecular labeling, by determining that the genotype of the chicken SNP site carries out Seedling selection to chicken Carcass Traits, production cost can be saved and accelerate genetic progress, it is preferably applied in a breed of chicken, with very big Economic Application value and scientific research value.
Description
Technical field
The present invention relates to technical field of gene detection, in particular to a kind of SNP marker relevant to chicken Carcass Traits
And its application.
Background technique
MicroRNA (miRNA) is that there are about the endogenous non-coding single stranded RNAs of 22 or so nucleotide for one kind.With molecule
The fast development of biological study technology and method, researcher have found more and more miRNA.MiRNA is by a segment length
For 70~80 nucleotide and comprising being generated after the shearing of the miRNA precursor (pre-miRNA) of one section of hairpin structure.MiRNA is logical
Crossing 3 ' end non-coding region (3-untranslated region, 3 ' UTR) complementations with its target gene mRNA leads to the mRNA
Translation be suppressed and realize regulation to gene.Although miRNA non-direct coding protein, the RNA that it is encoded is in biology
Entire life process in play an important role.Have now been found that miRNAs can participate in many adjusting approach, including skeletal muscle
Development, the differentiation of virus defense mechanism, hematopoiesis, orga- nogenesis, cell Proliferation and death, fat metabolism etc..
In recent years, with the rapid development of the technologies such as genomics and whole-genome association, miRNA already becomes
Popular domain in domestic and international Animal Genetics research.At home, Jin Jing etc. is using high throughput sequencing technologies to Holstein cow
Lactation period high milk-quality milk cow (H) and lactation period low milk-quality milk cow (L) breast tissue have carried out miRNA sequencing, pass through difference
Expression analysis screens group difference miRNAs, obtains 56 differential expression miRNA (P < 0.05), and to differential expression miRNA into
Row microRNA target prediction, by being screened to target gene, it was found that 4 have been reported the functional gene being closely related with lactoprotein, butterfat:
CSN3, SCD, LALBA and DGAT2.The biological function majority of target gene aggregation takes part in protein and fat metabolism, mammary gland hair
It educates and breaks up and immune function.Ling Yinghui etc. carries out Solexa sequencing to goat musculature and carries out bioinformatics point
Analysis identifies guard between 517 species and 2 distinctive miRNA of goat genome altogether.This seminar early period is pre- by target
It surveys software prediction and the target site that real GHR and IGF2BP3 is let-7b, let- is tested by Dual-Luciferase target checking system
The mrna expression amount that the overexpression of 7b will lead to GHR, IGF2BP3 is obviously lowered, it was demonstrated that let-7b has GHR, IGF2BP3 gene
There is negative regulation effect, meanwhile, discovery let-7b influences JAK-STAT signal path by mediating GHR gene, to regulate and control
The growth and development of skeletal muscle.There are many more reports to show that miRNAs widely participates in the growth and development, sexal maturity, mind of chicken embryo
In proliferation and a series of vital movements such as differentiation and cell migration [29-33] through cell and muscle cell.
Single nucleotide polymorphism (Single Nucleotide Polymorphisms, SNP) refers to living individual genome
The mutation that single nucleotide acid occurs in sequence.The frequency that SNPs occurs on DNA level is very high, high in all mutant forms
Up to ninety percent or more, but wherein, many mutation all concentrate on noncoding region, other seldom mutation are distributed in the code area of gene.Have
Evidence shows that SNP can have an impact RNA, DNA and protein level to the expression of gene and function, is to lead to individual and group
Between body includes disease, the key factor of medicaments insensitive and phenotypic difference etc., the SNP on mature body and precursor sequence
There is the target site for changing the mature body of its forming process even change or change the bond strength with target gene, causes miRNA's
Function changes, to cause the variation of animal character.Each miRNA has more than one target site, this means that
SNP on miRNA has great significance, therefore screens the SNPs in miRNA maturation body sequence and precursor sequence, discloses
Its function gets a good eye meaning.Current lookup screening SNP is carried out with sanger sequencing or PCR-RFLP method, by
Genechip technology and High-throughput lower in the efficiency of these technical methods, rapidly developing in recent years
Sequencing can carry out efficient screening mutational site at the genomic level.Research shows that being located at miRNA seed zone
SNP it is even more rare, and the SNP quantity that is present in miRNA prosoma is more is widely used in genetic diagnosis, tumor susceptibility
The fields such as property.
The gene polymorphic of MiRNA can influence to transcribe by changing the target site of miRNA and changing the stability of mRNA
Regulation after preceding and transcription then influences the expression of gene and the difference of phenotype.These polymorphic sites are expected as molecule mark
Note is for the assisted Selection in breeding.Zhao etc. research 313 be only positioned at sheep exon 2 RXRG gene mutation and its with it is double
It is more to analyze RXRG exon 1, exon 2, the gene of exon10 by PCR-SSCP for the relationship of tire character
State property, the results showed that, 3 kinds of P2 fragment genotypes are significant related (P < 0.05) to the twining trait in group.It is blue virtuous brave etc. to pass through
45 parts of Inner Mongolia White Cashmere Goat α-lactalbumin (LALBA) genes carry out sequencing and parting, and with cashmere amount, cashmere is thick, cashmere
Long and weight character is associated analysis.The results show that its exon 3 region there are 1 mutational site g.1897T > C.The position
There are significant related (P < 0.05), TC genotype individuals velour yield ratio TT genotype individuals to velour yield for point different genotype
Voluminous suede 142.68g, up to 26.21% (P < 0.05).Lei etc. (2010) experiments have shown that on miR27a gene T/C mutation with
The litter size of pig is related.Huang carries out 187 ducks using PCR restriction fragment length polymorphism and micro sequence method
SNP Genotyping.The hatching rate of CC genotype and TT genotype is respectively 79.59 ± 3.40 and 76.35 ± 1.77, significantly high
In CT genotype (65.77 ± 2.07) (P < 0.0 5).
Let-7 family is a kind of miRNA found by Reinhart et al. in the beginning of this century.Let-7 family member difference
On several different chromosomes.Research finds the gene of the let-7b targeting adjusting of one of let-7 family member in many
Playing an important role in signal path, many signal paths are related with the Proliferation and apoptosis of cell, therefore grind there are many researcher
Study carefully the relationship between let-7b and cell Proliferation.The researchs such as Sch μ Ltz discovery let-7b inhibits the cell of melanoma cells
Cycle progression and growth independent of anchoring.Zhao etc. find under study for action let-7b can by targeting stem cell adjust because
Sub- TLX and cell cycle regulatory factors cyclinD1 adjust the proliferation and differentiation of neural stem cell；Knockout then promotes nerve cord
Cell Proliferation promotes Neural Differentiation；And overexpression let-7b can reduce nerve stem cell proliferation.Chen Nan is in zebra fish is tested
It was found that the expression for inhibiting hypoxic inducing factor-1 (hif-1a) to will lead to let-7b is lowered, let-7b is overexpressed in ZF4 cell
Cell cycle process can be changed, G1 phase to the S phase is excessive in arresting cell cycle, stops or slow down the proliferation of cell；Also pass through
Experiment in vitro identifies that the target gene foxh1 of let-7b is also direct or indirect to take part in proliferation process.Qu Jie pass through by
MiRlet-7b and anti-miRlet-7b carries out scratch experiment after transfecting to MCF-7 Breast Cancer Cell, as the result is shown let-7b energy
The transfer ability of MCF-7 Breast Cancer Cell is enough significantly inhibited, and anti-miR let-7b group can remarkably promote breast cancer MCF-
The migration of 7 cells, it was demonstrated that let-7b is able to suppress the transfer ability of MCF-7 Breast Cancer Cell.
Summary of the invention
It is an object of the invention to determine in chicken let-7b prosoma gene order mutational site g.526G > genotype of A
To carry out Seedling selection to chicken Carcass Traits, saving production cost and to accelerate genetic progress, a kind of and chicken Carcass Traits are proposed
Relevant molecular labeling and its application.
The technical solution used in the present invention is: a kind of SNP marker relevant to chicken Carcass Traits, the SNP
Molecular labeling correspond to the mutational site in chicken let-7b prosoma gene order g.526G > A, the genotype of the SNP site is
AA, GA and GG.
Application of the SNP marker in chicken genetic breeding.
The method of one breeder Carcass Traits Seedling selection, according to the genotype of above-mentioned SNP site to the Carcass Traits of chicken
Seedling selection is carried out, is included the following steps:
1) chicken blood DNA to be measured is extracted；
2) chicken let-7b prosoma gene order is obtained from chicken blood DNA to be measured；
3) using DNA mix pond sequencing approach detection chicken let-7b prosoma gene order on SNP mutation site g.526G > A
Genotype；
4) Seedling selection is carried out based on Carcass Traits of the genotype of step 2) SNP site to chicken, wherein AA genotype
Body das Beinfleisch is great in GA and GG genotype individuals.
The beneficial effects of the present invention are: SNP marker of the invention, i.e., prominent in chicken let-7b prosoma gene order
Displacement point g.526G > A is related to chicken Carcass Traits, is a new molecular labeling, by the genotype for determining the chicken SNP site
Seedling selection is carried out to chicken Carcass Traits, production cost can be saved and accelerate genetic progress, be preferably applied for a breed of chicken
In, with very big Economic Application value and scientific research value.
Detailed description of the invention
Fig. 1 is primer specificity test result in SNP detection；
Fig. 2 is SNP site abrupt information in chicken let-7b prosoma gene order.
Specific embodiment
The present invention is specifically described below with reference to embodiment, in order to technical field personnel to of the invention
Understand.It is necessary to it is emphasized that embodiment is only intended to, the present invention will be further described herein, should not be understood as to this
The limitation of invention protection scope, fields person skilled in the art, the non-intrinsically safe that the present invention is made according to foregoing invention content
The modifications and adaptations of property, should still fall within protection scope of the present invention.Mentioned raw materials following simultaneously are unspecified, are
Commercial product；The processing step or preparation method not referred in detail be processing step known to a person skilled in the art or
Preparation method.
Embodiment
For the F2 that chicken to be measured is apricot bramble finch and recessive White Rock chicken giblets hand over for broiler chicken, quantity is 320, is supported using flat
Feeding manner, feeding meet the corn-soybean meal feed of international formula standard, and recessive White Rock chicken is a kind of fast large-scale broiler chicken,
And apricot bramble finch is the kind broiler chicken in Chinese Guangdong native country.Then measured respectively in 1~8 week old of chick its weight (BW,
BodyWeight, unit g).Carry out butchering sampling at 3 monthly age, record respectively they shin length, wide, chest breadth, chest depth,
Body length, chest angular width butcher weight, cortical thickness, remove internal organ weight, complete half net thorax weight, chest muscle leg flesh weight, plumage weight, abdominal fat weight, neck
Weight, heart weight, liver weight, stomach weight and small intestine are long.Its shoulder is measured with chest angle device；Subcutaneous fat depth shin is long, fat is wide, it is wide,
The long vernier caliper measurement of chest breadth, chest depth, body；The genomic DNA of all individuals is extracted according to conventional phenol/chloroform method, detects matter
It is diluted to 50ng/ μ L after amount and concentration, 4 DEG C save backup.
One, apricot flower × recessive white F2 is extracted for resource population family full-sibs chicken blood DNA to be measured
(1) 100 μ L blood are extracted, 500 μ L buffer TE and 10 μ L Proteinase Ks are added, vibrate 30min, 2 μ L are added and split
55 DEG C of liquid digestion of solution are overnight；
(2) 600 μ L Tris saturated phenols are added, then vibrate 15s, 10000rpm is centrifuged 8min；
(3) supernatant is taken, isometric Tris saturated phenol is added, then vibrates 15s, 10000rpm is centrifuged 8min；
(4) supernatant is taken, it is phenol/chloroform/isoamyl alcohol=25:24:1 mixed liquor that isometric ratio, which is added, 10s is vibrated,
10000rpm is centrifuged 8min；
(5) supernatant is taken, isometric chloroform/isoamyl alcohol (24:1) is added, vibrates 10s, 10000rpm is centrifuged 8min；
(6) supernatant is taken, the dehydrated alcohol that 2 times of volumes pre-cool is added, 12000rpm is centrifuged 10min；
(7) supernatant is abandoned, 70% ethyl alcohol of 1mL is added and is rinsed, 12000rpm is centrifuged 10min；
(8) supernatant is abandoned, 2h is spontaneously dried, suitable buffer TE is added and is saved backup in 4 DEG C.
Two, SNP detection and DNA mix pond sequencing
1, SNP is detected:
From https: //www.ncbi.nlm.nih.gov/ finds let-7b prosoma gene order, and both wings sequence respectively expands
500bp is opened up, goes out primer pair, primer pair information (primer sequence 5 ' as shown in table 1 using 5.0 software design of Primer premier
→ 3 ') the primer pair sequence of design, is sent to the synthesis of Qing Ke biotech firm, and PCR is carried out to blood DNA with the primer pair, is surveyed
The specificity for trying primer, as a result shown in attached drawing 1.
The primer information of 1 let-7b prosoma SNP of table screening
| F:GTAAATCCACCTAGCGT |
| R:GCCCAGGAAATATGAA |
2, DNA mixes pond sequencing:
It selects 30 DNA samples at random from the DNA gross sample of extraction and constructs mixed pond, (each sample is about for every three samples
0.33 μ L) it is mixed into a mixing sample, amount to 10.Mixed pond sample is subjected to PCR amplification, the primer and above-mentioned SNP are examined
Primer pair used in survey is identical, the PCR product of acquisition is sent to the sequencing of Sheng Gong biotech firm, sequencing result detects to obtain chicken
G.526G > A, sequencing result analysis are as shown in Fig. 2 in SNP mutation site in let-7b prosoma gene order.
Three, the genotype of let-7b prosoma mutational site g.526G > A is determined
By all chickens to be measured of embodiment, all progress PCR specific amplification obtains let-7b precursor to totally 320 parts of blood DNAs
PCR product, used primer pair information are as shown in table 1), it is for statistical analysis available by the individual to each genotype
The genotype frequency and gene frequency in the site.G.526G the gene of > A and genotype frequency count in the SNP mutation site
Structure is as shown in table 2 below.Wherein:
(1) genotype frequency refers to that a certain genotype individuals number accounts for the ratio that genotype is summarized in group:
Genotype frequency=certain genotype sum/group sum × 100%；
(2) gene frequency refers to that a certain gene in group accounts for the ratio of its same site full gene:
Same locus gene sum × 100% in gene frequency=certain gene number/group.
G.526G the gene of > A and genotype frequency count in table 2 let-7b prosoma mutational site
AA genotype is chicken community superiority genotype to be measured it can be seen from 2 structure of table.
Four, the association analysis of SNP mutation site g.526G > A and the Carcass Traits of chicken
1, g.526G the Carcass Traits association analysis of the site > A is as shown in table 3 below for let-7b prosoma.By will g.526G >
A site mutation information and carcass data relation analysis, wherein the site is presented significant related again to das Beinfleisch.The result of multiple comparisons is aobvious
Show that AA genotype individuals das Beinfleisch is noticeably greater than GA and AA genotype individuals again, implies that the site may increase with muscle development and cell
It is related to grow function.
The g.526G site the > A Carcass Traits association analysis of 3 let-7b prosoma of table
| Carcass Traits | AA | GA | GG | P value |
| Slaughter weight (kg) | 1.51±0.02(248) | 1.44±0.04(37) | 1.52±0.10(5) | 0.3693 |
| Slaughter traits (g) | 1334.4±17.5(248) | 1297.3±44.1(37) | 1344.7±97.5(5) | 0.7035 |
| Subcutaneous fat depth (mm) | 4.03±0.13(248) | 4.01±0.32(37) | 3.14±0.71(5) | 0.4516 |
| Fatty bandwidth (mm) | 12.13±0.29(246) | 11.89±0.73(37) | 12.13±1.61(5) | 0.9523 |
| Half net thorax weight (g) | 1223.5±15.3(247) | 1149.1±38.0(37) | 1236.3±84.1(5) | 0.1639 |
| Complete net thorax weight (g) | 1058.5±13.6(247) | 997.2±34.0(37) | 1044.0±75.1(5) | 0.2578 |
| Brisket weight (g) | 91.1±1.2(248) | 89.4±3.0(37) | 97.3±6.7(5) | 0.467 |
| Das Beinfleisch weight (g) | 116.9±1.4a(247) | 108.2±3.6b(37) | 120.9±7.9ab(5) | 0.0445 |
| Wing weight (g) | 65.1±0.8(247) | 62.6±2.0(37) | 70.6±4.4(5) | 0.1361 |
| Abdominal fat weight (g) | 29.4±1.4(247) | 22.7±3.5(37) | 22.3±7.7(5) | 0.2184 |
| Head, neck weight (g) | 124.0±1.8(247) | 117.5±4.6(37) | 123.1±10.1(5) | 0.4255 |
| Shin, pawl weight (g) | 29.4±0.4(247) | 27.8±1.1(37) | 31.8±2.4(5) | 0.1232 |
| Conscience muscular stomach glandular stomach weight (g) | 68.3±0.8(246) | 65.9±2.1(37) | 69.9±4.6(5) | 0.4693 |
| Small intestinal length (cm) | 139.1±1.1(248) | 135.5±2.7(37) | 136.2±6.0(5) | 0.482 |
| Abdominal fat (%) | 2.198±0.097(248) | 1.823±0.244(37) | 1.623±0.540(5) | 0.329 |
| Leg flesh rate (%) | 8.738±0.078(248) | 8.332±0.197(37) | 8.923±0.436(5) | 0.1052 |
| Chest muscle rate (%) | 6.862±0.072(248) | 6.976±0.181(37) | 7.267±0.400(5) | 0.6009 |
| Leg chest ratio (%) | 1.285±0.011(248) | 1.210±0.028(37) | 1.255±0.062(5) | 0.0548 |
Above-described embodiment is the preferred embodiment of the present invention, all with similar technique of the invention and made equivalence changes,
It should belong to protection category of the invention.
Claims (4)
1. a kind of SNP marker relevant to chicken Carcass Traits, it is characterised in that: the SNP marker corresponds to chicken
Mutational site in let-7b prosoma gene order g.526G > A, the genotype of the SNP site is AA, GA and GG.
2. application of the SNP marker described in claim 1 in chicken genetic breeding.
3. the method for breeder growth Meat Traits Seedling selection, which is characterized in that SNP site according to claim 1
Genotype Seedling selection is carried out to the Carcass Traits, Meat Quality and body measurement trait of chicken.
4. the method for breeder Carcass Traits Seedling selection according to claim 3, which is characterized in that including walking as follows
It is rapid:
1) chicken blood DNA to be measured is extracted；
2) chicken let-7b prosoma gene order is obtained from chicken blood DNA to be measured；
3) using DNA mix in pond sequencing approach detection chicken let-7b prosoma gene order SNP mutation site g.526G > base of A
Because of type；
4) Seedling selection is carried out based on Carcass Traits of the genotype of step 2) SNP site to chicken, wherein AA genotype individuals leg
Meat is great in GA and GG genotype individuals.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112662782A (en) * | 2020-12-30 | 2021-04-16 | 华南农业大学 | Chicken slaughter trait related TMEM18 gene molecular marker and application |
| CN113789393A (en) * | 2021-10-12 | 2021-12-14 | 南昌师范学院 | Molecular marker related to chicken carcass traits and application thereof |
| CN114717331A (en) * | 2022-04-24 | 2022-07-08 | 华南农业大学 | Poultry SNP molecular marker selection and application thereof |
| CN114807384A (en) * | 2022-04-14 | 2022-07-29 | 华南农业大学 | SNP molecular marker related to chicken carcass traits and application thereof |
| CN114908176A (en) * | 2022-05-18 | 2022-08-16 | 华南农业大学 | Molecular marker related to chicken carcass and growth traits and application thereof |
| CN115125308A (en) * | 2022-06-07 | 2022-09-30 | 华南农业大学 | SNP molecular marker for genetically improving chicken carcass traits |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105779607A (en) * | 2016-04-13 | 2016-07-20 | 南昌师范学院 | Application of chicken GnRHR-2 gene as molecular mark of chicken reproduction traits |
| CN106086229A (en) * | 2016-08-26 | 2016-11-09 | 广东省农业科学院动物科学研究所 | Molecular marker that chicken growth traits is relevant and discrimination method thereof and application |
| WO2016179530A1 (en) * | 2015-05-06 | 2016-11-10 | Seracare Life Sciences, Inc. | Liposomal preparations for non-invasive-prenatal or cancer screening |
| CN106636367A (en) * | 2016-11-25 | 2017-05-10 | 浙江省农业科学院 | Molecular genetic marker related with egg laying performance of hens and application |
-
2018
- 2018-10-17 CN CN201811207113.3A patent/CN109182542B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016179530A1 (en) * | 2015-05-06 | 2016-11-10 | Seracare Life Sciences, Inc. | Liposomal preparations for non-invasive-prenatal or cancer screening |
| CN105779607A (en) * | 2016-04-13 | 2016-07-20 | 南昌师范学院 | Application of chicken GnRHR-2 gene as molecular mark of chicken reproduction traits |
| CN106086229A (en) * | 2016-08-26 | 2016-11-09 | 广东省农业科学院动物科学研究所 | Molecular marker that chicken growth traits is relevant and discrimination method thereof and application |
| CN106636367A (en) * | 2016-11-25 | 2017-05-10 | 浙江省农业科学院 | Molecular genetic marker related with egg laying performance of hens and application |
Non-Patent Citations (4)
| Title |
|---|
| ENSEMBL: "rs10724042", 《ENSEMBL》 * |
| 岳孝亭等: "microRNA let-7b靶向IGF2BP3调控鸡成肌细胞增殖的研究", 《中国家禽》 * |
| 王乐乐: "鸡let-7a的组织表达、功能预测及其靶基因ADIPOR2遗传效应分析", 《中国优秀硕士学位论文全文库 农业科技辑》 * |
| 罗文: "miRNAs介导的信号通路对鸡成肌细胞增殖和分化的调控", 《中国博士学位论文全文库 农业科技辑》 * |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112662782A (en) * | 2020-12-30 | 2021-04-16 | 华南农业大学 | Chicken slaughter trait related TMEM18 gene molecular marker and application |
| CN112662782B (en) * | 2020-12-30 | 2023-03-14 | 华南农业大学 | Chicken slaughter trait related TMEM18 gene molecular marker and application thereof |
| CN113789393A (en) * | 2021-10-12 | 2021-12-14 | 南昌师范学院 | Molecular marker related to chicken carcass traits and application thereof |
| CN113789393B (en) * | 2021-10-12 | 2023-06-16 | 南昌师范学院 | Molecular marker related to chicken carcass traits and application thereof |
| CN114807384A (en) * | 2022-04-14 | 2022-07-29 | 华南农业大学 | SNP molecular marker related to chicken carcass traits and application thereof |
| CN114807384B (en) * | 2022-04-14 | 2022-11-25 | 华南农业大学 | SNP molecular marker related to chicken carcass traits and application thereof |
| CN114717331A (en) * | 2022-04-24 | 2022-07-08 | 华南农业大学 | Poultry SNP molecular marker selection and application thereof |
| CN114717331B (en) * | 2022-04-24 | 2024-07-02 | 华南农业大学 | Poultry SNP molecular marker selection and application thereof |
| CN114908176A (en) * | 2022-05-18 | 2022-08-16 | 华南农业大学 | Molecular marker related to chicken carcass and growth traits and application thereof |
| CN114908176B (en) * | 2022-05-18 | 2022-12-09 | 华南农业大学 | Molecular marker related to chicken carcass and growth traits and application thereof |
| CN115125308A (en) * | 2022-06-07 | 2022-09-30 | 华南农业大学 | SNP molecular marker for genetically improving chicken carcass traits |
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