CN109179667A - A kind of method that sleeping stem red-spotted stonecrop-microbial association repairs Riparian Zone runoff rainwater - Google Patents
A kind of method that sleeping stem red-spotted stonecrop-microbial association repairs Riparian Zone runoff rainwater Download PDFInfo
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- CN109179667A CN109179667A CN201811099585.1A CN201811099585A CN109179667A CN 109179667 A CN109179667 A CN 109179667A CN 201811099585 A CN201811099585 A CN 201811099585A CN 109179667 A CN109179667 A CN 109179667A
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- Prior art keywords
- spotted stonecrop
- riparian zone
- repairs
- runoff rainwater
- sleeping
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/001—Runoff or storm water
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A20/00—Water conservation; Efficient water supply; Efficient water use
- Y02A20/40—Protecting water resources
- Y02A20/402—River restoration
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- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Organic Chemistry (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to the methods that a kind of sleeping stem red-spotted stonecrop-microbial association repairs Riparian Zone runoff rainwater.Complex microbial inoculum is put into riverbank topsoil, rotary tillage is puddled uniformly, is uniformly sprinkled into sleeping stem red-spotted stonecrop stem section later, and watering keeps soil conservation wet.Using method of the invention, the total nitrogen in Riparian Zone runoff rainwater, total phosphorus, five-day BOD, COD are repaired using plant and microorganism synergistic effect, Riparian Zone runoff rainwater restorative procedure effect of the invention is good, cheap convenience, is easily managed and operates, secondary pollution will not be generated, is with a wide range of applications.
Description
Technical field
The present invention relates to the methods that a kind of sleeping stem red-spotted stonecrop-microbial association repairs Riparian Zone runoff rainwater, belong to ambient water
Body pollution Treatment process field.
Background technique
The city river component part important as urban ecological system, is of great significance to the ecological construction in city.
As the rapid development of sociaty and economy, the middle and small river water pollution problems in city is got worse.Living consumption significantly improves,
Production, sanitary wastewater, waste discharge amount increase rapidly, and the pollution of area source as caused by rainfall runoff is also just even more serious.
The governing measure of removal water pollutant is broadly divided into chemical remediation technology, peripheral doses technology and biological prosthetic skill
Art.Bioremediation technology is the emerging technology for the cleaning ambient that new development is got up, it is using particular organisms to dirty in water body
Absorption, conversion or the degradation for contaminating object reach the biological control measure for slowing down or finally eliminating water pollution, restore water ecology function.
Polluted-water pollutant is mainly to pass through the metabolic activity of microorganism for its Degradation and Transformation.
Therefore, the concentration that runoff rain water pollutant is effectively reduced in a kind of bioremediation technology is found, prevents runoff rainwater dirty
Contaminating river water and underground water has realistic meaning.
Summary of the invention
In view of the deficiencies of the prior art, the present invention provides a kind of sleeping stem red-spotted stonecrop-microbial associations to repair Riparian Zone runoff rain
The method of water, the method are that one kind is easy to operate, low in cost, repairing effect is good, does not cause the biological restoration of secondary pollution,
The concentration that can be effectively reduced runoff rain water pollutant prevents runoff rainwater pollution river water and underground water.
To achieve the above object, the technical solution adopted by the present invention is that: a kind of sleeping stem red-spotted stonecrop-microbial association reparation pollution
The method of river water, comprises the following steps that
1) atomizing watering is carried out to Riparian Zone, keeps soil moisture content；
2) complex microbial inoculum is mixed into the soil of Riparian Zone surface layer 15~25cm depth, rotary tillage is puddled uniformly；
3) sleeping stem red-spotted stonecrop stem section is uniformly sowed, atomizing watering keeps the moisture content of soil.
The method that a kind of above-mentioned sleeping stem red-spotted stonecrop-microbial association repairs Riparian Zone runoff rainwater, the microorganism are multiple
The input amount of combined bacteria agent is 37~45g/m2。
The method that a kind of above-mentioned sleeping stem red-spotted stonecrop-microbial association repairs Riparian Zone runoff rainwater, described in step 3)
The length of sleeping stem red-spotted stonecrop stem section is 2-5cm.
The method that a kind of above-mentioned sleeping stem red-spotted stonecrop-microbial association repairs Riparian Zone runoff rainwater, described in step 3)
The application rate of sleeping stem red-spotted stonecrop is 400-600g/m2。
The method that a kind of above-mentioned sleeping stem red-spotted stonecrop-microbial association repairs Riparian Zone runoff rainwater, step 1) and step 3),
The moisture content of the holding soil is 30~40%.
The method that a kind of above-mentioned sleeping stem red-spotted stonecrop-microbial association repairs Riparian Zone runoff rainwater, the microorganism are multiple
Combined bacteria agent is by bacillus subtilis, bacillus megaterium, Pseudomonas stutzeri, vesicular-arbuscular mycorrhiza, Trichoderma viride and thin Huang
Streptomycete is mixed to prepare.
The method that a kind of above-mentioned sleeping stem red-spotted stonecrop-microbial association repairs Riparian Zone runoff rainwater, in mass ratio, withered grass bud
Spore bacillus: bacillus megaterium: Pseudomonas stutzeri: vesicular-arbuscular mycorrhiza；Trichoderma viride: streptomyces microflavus=1:(1-3):
(1-3): (4-8): (2-4): (2-4).
The method that a kind of above-mentioned sleeping stem red-spotted stonecrop-microbial association repairs Riparian Zone runoff rainwater, the microorganism are multiple
Combined bacteria agent the preparation method is as follows:
1) in mass ratio, yeast extract: NaCl: peptone: water=1:1-4:1-4:15-25, take yeast extract,
NaCl, peptone and water are uniformly mixed, and liquid fermentation liquid is made；
2) in mass ratio, bacillus subtilis: bacillus megaterium: Pseudomonas stutzeri: vesicular-arbuscular mycorrhiza: green wood
Mould: streptomyces microflavus=1:1-3:1-3:4-8:2-4:2-4 takes bacillus subtilis, bacillus megaterium, Amur vacation unit cell
Bacterium, vesicular-arbuscular mycorrhiza, Trichoderma viride and streptomyces microflavus, are placed in liquid fermentation liquid and cultivate, and obtain the liquid of each microbial bacteria
Body microbial inoculum；By bacillus subtilis liquid bacterial agent, bacillus megaterium liquid bacterial agent, Pseudomonas stutzeri liquid bacterial agent, vesicle
Arbuscular mycorrhiza liquid bacterial agent, Trichoderma viride liquid bacterial agent, streptomyces microflavus liquid bacterial agent are uniformly mixed, and it is compound to obtain liquid microbe
Microbial inoculum.
3) liquid microbe composite bacteria agent is freeze-dried, obtains the complex microbial inoculum.
The beneficial effects of the present invention are:
1) method that compared with prior art, the present invention uses is easy to operate, environment influence is small, not will lead to pollutant and turns
Move or formed secondary pollution.
2) facility used in the present invention is simple, and operating cost is low, can be with large-scale promotion application.
3) present invention absorbs, degradation runoff rainwater can not only reduce pollution of the diameter rainwater to river water, can also reduce diameter
Flow pollution of the rainwater to underground water.
4) Riparian Zone runoff rainwater of the invention reparation application method phase repairing effect is good, expense is low, is easily managed and grasps
Make, pollutant concentration can be reduced to greatest extent, is with a wide range of applications.
Detailed description of the invention
Fig. 1 Riparian Zone rainwash cuts dirty imitative experimental appliance；
Wherein, A is that water-collecting tank water is leading, slot tap based on B, and slot based on C, D is catch basin.
Specific embodiment
The present invention is described in further details below by example.Test method in following embodiments is such as without special theory
It is bright, it is conventional method, experimental material used is unless otherwise specified to be commercially available from conventional biochemical reagent market.
Embodiment 1 crouches the method that stem red-spotted stonecrop-microbial association repairs Riparian Zone runoff rainwater
The present embodiment is for repairing Hunhe Riparian Zone runoff rainwater.
Take Hunhe Riparian Zone runoff rainwater in the bucket of sampling, by national quality standard of water environment, regulating water quality
Index, make contaminant water in it is light, in, three kinds of degree of severe, be respectively designated as 1#, 2#, 3#, water sample situation such as table 1, by original sample runoff
Rainwater is named as 0# as reference.
Table 1
(1) preparation of complex microbial inoculum:
1) in mass ratio, yeast extract: NaCl: peptone: water=1:2:2:19, by yeast extract, NaCl, albumen
Peptone and water are uniformly mixed, and liquid fermentation liquid is made.
2) in mass ratio, bacillus subtilis: bacillus megaterium: Pseudomonas stutzeri: vesicular-arbuscular mycorrhiza: green wood
It is mould: streptomyces microflavus=1:2:2:6:3:3, respectively by bacillus subtilis, bacillus megaterium, Pseudomonas stutzeri, vesicle
Arbuscular mycorrhiza, Trichoderma viride and streptomyces microflavus are inoculated in liquid fermentation liquid by 5% inoculum concentration, are trained in 30-35 DEG C of fermentation
Support 1-2 days, respectively obtain the liquid bacterial agent of each microbial bacteria.
3) by bacillus subtilis liquid bacterial agent, bacillus megaterium liquid bacterial agent, Pseudomonas stutzeri liquid bacterial agent, bubble
Capsule arbuscular mycorrhiza liquid bacterial agent, Trichoderma viride liquid bacterial agent and streptomyces microflavus liquid bacterial agent are uniformly mixed, obtain the micro- life of liquid
Object composite bacteria agent.
4) liquid microbe composite bacteria agent is freeze-dried, obtains complex microbial inoculum.
(2) method
1, the preparation of experimental provision: experimental provision is made of organic glass, as shown in Figure 1, body groove C is in groove type, it is high
500mm, long 2500mm, width 250mm, the upper end body groove C two sides are respectively arranged with catch basin D, high 170mm, long 250mm, width
250mm, the top catch basin D are concordant with the top body groove C.Body groove lower end side is provided with body groove tap B, with body groove
Tap B, which is corresponded to, is provided with water-collecting tank water faucet A on the catch basin of side.During the experiment, the position of body groove tap
It will be lower than the upper surface of the soil added in body groove.
Lowest level is the thick gravel of 2cm or so in body groove C, and thick gravel upper berth geotextiles are to stop under particulate matter entrance
Layer, geotextiles top are the soil horizon of 45cm thickness.Soil is acquired from Hun River Shenyang Section.
2, atomizing watering is carried out into soil horizon, and soil moisture content is made to reach 35%.
4, complex microbial inoculum is mixed into the soil of about 20cm depth to upper soll layer, rotary tillage is puddled uniformly.Microorganism
The input amount of composite bacteria agent is 37~45g/m2。
5, taking length is the sleeping stem red-spotted stonecrop stem section of 3-4cm, and uniformly sowing carries out atomizing watering on soil after compacting,
The moisture content of soil is set to reach 40%.The application rate of sleeping stem red-spotted stonecrop stem section is 400-600g/m2。
(3) effect
1. 0#, 1#, 2#, 3# Wastewater Sample slowly are poured into the catch basin D without tap side of experimental provision respectively,
Enter sewage in a manner of overflow in body groove C, a part of sewage is seeped into soil under passing through, and shape interflow subsurface drainage is from body groove
The body groove tap B of bottom is discharged separating device；Another part forms rainwash on surface, and final overflow enters body groove
The other side C is equipped in the catch basin of catch basin fire hose A, from separating device from water-collecting tank water faucet A.It is taken below tap A, B
Sample simultaneously mixes, and every kind of Wastewater Sample samples 3 times.Water sample is put into ready cleaning plastic bottle, is saved under 4 degrees Celsius, respectively
It is detected, as a result such as table 2.
Table 2
As can be seen from Table 2, stem red-spotted stonecrop-microbial association reparation of crouching reaches the reduction rate of the water total nitrogen of serious pollution
67.0%, 73.7% is reached to the reduction rate of the total nitrogen of runoff rainwater；88.2% is reached to the total phosphorus reduction rate of serious pollution；It is right
The total phosphorus reduction rate of runoff rainwater reaches 93.9%, and the reduction rate of COD reaches 68.2% or so, needs to biochemistry on the five
The reduction rate of oxygen amount reaches 58.6% or so.
Claims (8)
1. the method that a kind of sleeping stem red-spotted stonecrop-microbial association repairs Riparian Zone runoff rainwater, which is characterized in that including walking as follows
It is rapid:
1) atomizing watering is carried out to Riparian Zone, keeps soil moisture content；
2) complex microbial inoculum is mixed into the soil of 15~25cm depth to Riparian Zone surface layer, rotary tillage is puddled uniformly；
3) sleeping stem red-spotted stonecrop stem section is uniformly sowed, atomizing watering keeps the moisture content of soil.
2. the method that a kind of sleeping stem red-spotted stonecrop according to claim 1-microbial association repairs Riparian Zone runoff rainwater, special
Sign is that the input amount of complex microbial inoculum is 37~45g/m in step 2)2。
3. the method that a kind of sleeping stem red-spotted stonecrop according to claim 1-microbial association repairs Riparian Zone runoff rainwater, step
3) length of the sleeping stem red-spotted stonecrop stem section described in is 2-5cm.
4. the method that a kind of sleeping stem red-spotted stonecrop according to claim 1-microbial association repairs Riparian Zone runoff rainwater, step
3) application rate of the sleeping stem red-spotted stonecrop stem section described in is 400-600g/m2。
5. the method that a kind of sleeping stem red-spotted stonecrop according to claim 1-microbial association repairs Riparian Zone runoff rainwater, special
Sign is that holding soil moisture content described in step 1) and step 3) is 30~40%.
6. the method that a kind of sleeping stem red-spotted stonecrop according to claim 1-microbial association repairs Riparian Zone runoff rainwater, special
Sign is, complex microbial inoculum described in step 2) be by bacillus subtilis, bacillus megaterium, Pseudomonas stutzeri,
Vesicular-arbuscular mycorrhiza, Trichoderma viride and streptomyces microflavus are mixed to prepare.
7. the method that a kind of sleeping stem red-spotted stonecrop according to claim 6-microbial association repairs Riparian Zone runoff rainwater, special
Sign is, in mass ratio, bacillus subtilis: bacillus megaterium: Pseudomonas stutzeri: vesicular-arbuscular mycorrhiza；Trichoderma viride:
Streptomyces microflavus=1:(1-3): (1-3): (4-8): (2-4): (2-4).
8. the method that a kind of sleeping stem red-spotted stonecrop according to claim 7-microbial association repairs Riparian Zone runoff rainwater, special
Sign is, the complex microbial inoculum the preparation method is as follows:
1) in mass ratio, yeast extract: NaCl: peptone: water=1:1-4:1-4:15-25, take yeast extract, NaCl,
Peptone and water are uniformly mixed, and liquid fermentation liquid is made；
2) in mass ratio, bacillus subtilis: bacillus megaterium: Pseudomonas stutzeri: vesicular-arbuscular mycorrhiza: Trichoderma viride:
Streptomyces microflavus=1:1-3:1-3:4-8:2-4:2-4 takes bacillus subtilis, bacillus megaterium, Pseudomonas stutzeri, bubble
Capsule arbuscular mycorrhiza, Trichoderma viride and streptomyces microflavus, are placed in liquid fermentation liquid and cultivate, and obtain the liquid bacteria of each microbial bacteria
Agent；By bacillus subtilis liquid bacterial agent, bacillus megaterium liquid bacterial agent, Pseudomonas stutzeri liquid bacterial agent, vesicle clump branch
Mycorhiza liquid bacterial agent, Trichoderma viride liquid bacterial agent, streptomyces microflavus liquid bacterial agent are uniformly mixed, and obtain liquid microbe compound bacteria
Agent；
3) liquid microbe composite bacteria agent is freeze-dried, obtains complex microbial inoculum.
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2018
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120024780A1 (en) * | 2010-06-21 | 2012-02-02 | Herman Carr | Water treatment plant for combined biomass and biogas production |
| CN102001752A (en) * | 2010-09-26 | 2011-04-06 | 江苏大学 | Method for building ecological interception ditch plant community by taking Phalaris canariensis as dominant species |
| CN102211820A (en) * | 2011-03-31 | 2011-10-12 | 北京师范大学 | Method for controlling area-source pollution |
| CN107724336A (en) * | 2017-11-16 | 2018-02-23 | 辽宁大学 | A kind of construction method of " phytomicroorganism " coupled mode landscape ecological shore protection suitable for north city inland river |
| CN107792946A (en) * | 2017-12-05 | 2018-03-13 | 郑州大学 | Solid shore spats component of vegetation type for micro- polluted river strengthened denitrification and preparation method thereof |
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Application publication date: 20190111 |