CN109170320A - A kind of laying cycle of laying hens premix and its application - Google Patents
A kind of laying cycle of laying hens premix and its application Download PDFInfo
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- CN109170320A CN109170320A CN201811057161.9A CN201811057161A CN109170320A CN 109170320 A CN109170320 A CN 109170320A CN 201811057161 A CN201811057161 A CN 201811057161A CN 109170320 A CN109170320 A CN 109170320A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/28—Silicates, e.g. perlites, zeolites or bentonites
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
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Abstract
The present invention provides a kind of laying cycle of laying hens premix and its application, main components are as follows: vitamin, microelement, amino acid, probiotics.The present invention improves dregs of beans protein utilization based on laying hen ideal amino acid profile, through make-up monomers amino acid, reduces use ratio of the dregs of beans in complete diet pellet.Animal experiment shows, the production performances such as laying hen egg size, laying rate and the feedstuff-egg ratio of the adequate diet raising prepared with premix of the present invention and conventional crude protein level daily ration are without significant difference, and since rate of ultilization of amino acid improves, the discharge of nitrogen significantly reduces in excrement, be conducive to improve environment of chicken house, it reduces environmental pollution, feed cost is effectively reduced, improve the overall economic efficiency of breeding layer chicken.
Description
Technical field
The present invention relates to a kind of laying hen premixes, and in particular to a kind of laying cycle of laying hens premix and its application.
Background technique
China's breeding layer chicken industry comes into transition and upgrade stage, feed and excrement after it experienced fast development at present
Two problems of this one-in-and-one-out seriously affect the sustainable development of the economic benefit and industry of breeding layer chicken.A large number of studies show that
Daily ration dregs of beans dosage is reduced, rate of ultilization of amino acid in dregs of beans is improved, the above problem can be effectively relieved by reducing the discharge of excrement ammonia nitrogen.
But if simply reducing daily ration dregs of beans dosage will affect the production performance of laying hen, mentioned using the high protein raw materials such as fish meal, meat meal tankage
Although high premix crude protein content can be to avoid the decline of production performance, there is no drop for total crude protein level in daily ration
It is low, it does so and not only increases feed cost, also will cause the waste of amino acid, increase the discharge of excrement ammonia nitrogen, pollute environment.
The present invention forms in conjunction with the amino acid of corn-soybean meal diet according to the ideal amino acid profile of laying hen, passes through benefit
These four essential amino acids of lysine, methionine, threonine, tryptophan are filled, daily ration amino acid composition is adjusted, makes daily ration lower
Meet the needs of laying cycle of laying hens is to amino acid in the case of dregs of beans dosage, and then improve Dietary Crude Protein utilization rate, is not reducing
While the production performances such as laying rate, egg size, the discharge of the odorous gas such as excrement ammonia nitrogen is reduced, while mitigating liver and kidney point
The burden of solution, excretion additional amino acid.For the digestibility for further increasing each ingredient in daily ration, the special addition composite microbial of the present invention
State preparation protects intestinal health while improving efficiency of feed utilization, enhances laying hen autoimmunity and anti-stress ability, reduces
The overall economic efficiency of breeding layer chicken stress be improved with the generation of disease.
Summary of the invention
It is special the purpose of the present invention is being formed according to the amino acid of laying hen ideal amino acid profile and corn-soybean meal diet
Point devises a kind of laying cycle of laying hens premix and its application by make-up monomers amino acid, while passing through lactobacillus plantarum
Effect saves protein raw materials, reduces fecal pollution, reduces laying hen liver kidney burden and enhancing immunity of layer chicken, supports to improve laying hen
The overall economic efficiency grown.
In order to achieve the above objectives, the present invention takes following technical scheme: the present invention provides a kind of laying cycle of laying hens premixs
Material, which is characterized in that its main component are as follows: vitamin, microelement, amino acid, lactobacillus plantarum.
The vitamin main component are as follows: vitamin A, vitamine D3, vitamin E, Vitamin K3, vitamin B1, dimension life
Two or more any compositions of plain B2, vitamin B6, vitamin B12, biotin, niacin, pantothenic acid, folic acid.
The microelement main component are as follows: cupric sulfate pentahydrate, ferrous sulfate, white vitriol, manganese sulfate monohydrate, acid iodide
Two or more any compositions of potassium, sodium selenite, zeolite powder.
The amino acid main component are as follows: two or more any compositions of lysine, methionine, threonine, tryptophan.
The present invention also provides a kind of application methods of laying cycle of laying hens premix, by laying cycle of laying hens premix and full price
Feed is used in mixed way according to mass ratio 5:95.
The lactobacillus plantarum of addition can secrete the amylase, protease and cellulase of high activity, promote diet nutrient
The digestion and absorption of substance.
The adequate diet crude protein being formulated with premix of the present invention according to recommended formula is 14.1%, it is therefore desirable to root
Methionine, lysine, threonine and tryptophan are additionally supplemented according to ideal protein model, first two is that laying hen first and second limits
Acidic amino acid participates in a variety of biochemical reactions of body, and threonine is related with laying hen immune system, and tryptophan is adjustable stress
The utilization rate of Dietary Crude Protein can be improved by the supplement of these four essential amino acids in the level of hormone, reduces excrement ammonia nitrogen
Discharge, while reducing feed cost improve pouity dwelling place environment, reduce the generation of respiratory disease.
Specific embodiment
The present invention is further illustrated below in conjunction with example.
The preparation of 1 microelement of embodiment
1, take 0.45 gram of cupric sulfate pentahydrate, 2.4 grams of ferrous sulfate be uniformly mixed, it is stand-by to be prepared into trace element premix agent.
2,0.45 gram of cupric sulfate pentahydrate, 3.5 grams of manganese sulfate monohydrate, 28 milligrams of Potassiumiodate, 7.5 milligrams of sodium selenite, micro- is taken
11.215 grams of secondary element carrier zeolite powder are uniformly mixed, and it is stand-by to be prepared into trace element premix agent.
3,3.5 grams of manganese sulfate monohydrate, 28 milligrams of Potassiumiodate, 7.5 milligrams of sodium selenite, trace vector zeolite powder are taken
11.215 grams are uniformly mixed, and it is stand-by to be prepared into trace element premix agent.
4, take 3.5 grams of manganese sulfate monohydrate, 7.5 milligrams of sodium selenite, 11.215 grams of trace vector zeolite powder mixing equal
It is even, it is stand-by to be prepared into trace element premix agent.
5,0.45 gram of cupric sulfate pentahydrate, 2.4 grams of ferrous sulfate, 7.4 grams of white vitriol, 3.5 grams of manganese sulfate monohydrate, iodine are taken
28 milligrams of sour potassium, 7.5 milligrams of sodium selenite, 11.215 grams of trace vector zeolite powder be uniformly mixed, be prepared into microelement
Pre-mixing agent is stand-by.
6,0.45 gram of cupric sulfate pentahydrate, 2.4 grams of ferrous sulfate, 3.5 grams of manganese sulfate monohydrate, 28 milligrams of Potassiumiodate, sub- selenium are taken
7.5 milligrams of sour sodium, 11.215 grams of trace vector zeolite powder be uniformly mixed, it is stand-by to be prepared into trace element premix agent
The preparation of 2 vitamin of embodiment:
1, vitamine D3: 110 milligrams, vitamin E: 280 milligrams, Vitamin K3: 25 milligrams, vitamin B1: 35 milligrams, vitamin
B2:110 milligrams, vitamin B6: 55 milligrams, vitamin B12: 0.3 milligram, biotin: 1.7 milligrams, niacin: 420 milligrams, general
Acid: 140 milligrams, folic acid: 12 milligrams, 3511 milligrams of vitamin carrier powdered rice hulls are uniformly mixed, and compound micro-ecological preparation is made and waits for
With.
2, take retinyl acetate: 300 milligrams, vitamine D3: 110 milligrams are uniformly mixed, and compound micro-ecological preparation is made
For use.
3, vitamine D3: 110 milligrams, vitamin E: 280 milligrams, Vitamin K3: 25 milligrams, vitamin B1 is taken: 35 milligrams,
Vitamin B2: 110 milligrams, vitamin B6: 55 milligrams, vitamin B12: 0.3 milligram, biotin: 1.7 milligrams, niacin: 420 millis
Gram, pantothenic acid: 140 milligrams, 3511 milligrams of vitamin carrier powdered rice hulls be uniformly mixed, it is stand-by that compound micro-ecological preparation is made.
4, vitamine D3: 110 milligrams, vitamin E: 280 milligrams, Vitamin K3: 25 milligrams, vitamin B1 is taken: 35 milligrams,
Vitamin B2: 110 milligrams, niacin: 420 milligrams, pantothenic acid: 140 milligrams, folic acid: 12 milligrams, 3511 milli of vitamin carrier powdered rice hulls
Gram be uniformly mixed, it is stand-by that compound micro-ecological preparation is made.
5, retinyl acetate: 300 milligrams, vitamine D3: 110 milligrams, vitamin E: 280 milligrams, Vitamin K3 is taken:
25 milligrams, vitamin B1: 35 milligrams, vitamin B2: 110 milligrams, vitamin B6: 55 milligrams, vitamin B12: 0.3 milligram, life
Object element: 1.7 milligrams, niacin: 420 milligrams, pantothenic acid: 140 milligrams, folic acid: 12 milligrams, 3511 milligrams of vitamin carrier powdered rice hulls are mixed
It closes uniformly, it is stand-by that compound micro-ecological preparation is made.
The preparation of 3 amino acid of embodiment
1, rely 3 milligrams of ammonia, 2 milligrams of methionine, 0.5 milligram of threonine, 3 milligrams of tryptophan.
2, rely 3 milligrams of ammonia, 2 milligrams of methionine.
3,2 milligrams of methionine, 0.5 milligram of threonine, 3 milligrams of tryptophan.
4,2 milligrams of methionine, 3 milligrams of tryptophan.
5, rely 3 milligrams of ammonia, 0.5 milligram of threonine, 3 milligrams of tryptophan.
6, rely 3 milligrams of ammonia, 2 milligrams of methionine, 0.5 milligram of threonine.
4 laying cycle of laying hens premix of embodiment preparation
The premix preparing complete feed prepared using the above method, by the microelement prepared, vitamin, amino acid and plant
Object lactobacillus is combined according to mass ratio 1:1:1:1, is prepared into laying cycle of laying hens premix.
The preparation of 5 adequate diet of embodiment
The laying cycle of laying hens premix prepared and corn, dregs of beans etc. are mixed according to mass ratio 5:95, form full price day
Grain.
6 verification test of embodiment
Feeding experiment and verifying:
Test grouping and design:
Blue brown commercial generation laying hen 10000 of 35 week old sea is selected, is randomly divided into test group and two groups of control group, every group of 5 repetitions,
1000 chickens of each repetition, the laying period premix that test group feeding is prepared using premix of the present invention, control group fed are conventional
The daily ration of crude protein ratio, pre-feeding period on August 13, to 19 days 2015, totally 7 days, 2015 experimental periods August 20 days to September 30
Day, totally 42 days
Feeding management: raising is required according to extra large blue brown commercial generation feeding management handbook.
Testing index:
(1) performance test:
During test, lay eggs weight, egg number are recorded as unit of repetition daily, and calculate average egg weight, egg production and laying rate,
Feed consumption rate and egg material ratio are calculated as unit of repetition weekly.
(2) Egg Quality measures:
Egg sample, each repetition are collected as unit of the pre- last day for raising all last days, the 3rd week experimental period, the 6th week is by repetition
15 pieces of eggs, for measuring eggshell strength, shell thickness, albumen height, yolk color, hangh unit, egg shape index, yolk
The Egg Qualities indexs such as ratio, yolk moisture content.
Egg Quality measuring method: assay balance claims shell egg, yolk and eggshell weight, and egg device separation yolk-egg white is simultaneously weighed,
Yolk mixes freeze-drying thoroughly, calculates yolk moisture content, while counting eggshell and yolk specific gravity.Eggshell strength uses eggshell strength analyzer
It is measured, shell thickness measures eggshell blunt end, tip and middle section with vernier caliper respectively and is averaged, and uses vernier caliper
Egg major diameter and minor axis are measured, egg shape index is calculated with draw ratio minor axis, dense albumen height is measured with albumen height analyzer, egg
Yellow color is fanned with colorimetric and is measured, and egg size, dense albumen height are brought into and are calculated according to formula by hangh unit.
(3) blood parameters measure:
It is raised week pre-, experimental period the 3rd week, 6 chickens similar in weight are chosen in the fixed cage position of repetition every respectively within the 6th week, and empty stomach wing is quiet
Arteries and veins blood sampling, is stored in heparin aluminium anticoagulant tube, and 10ul fully-automatic dry type Biochemical Analyzer is taken to measure whole blood ammonia density, remainder one
Half sample preparation blood plasma carries out Plasma Uric Acid Concentration Testing with phosphotungstic acid reduction method.
(4) measurement of fecal nitrogen and greenhouse gas emission:
Chicken slurry of feces and urine is collected daily in pre- feeding week, the repetition every respectively of the 6th week experimental period, is weighed and is recorded, 60-65 DEG C of baking
Case is dry to constant weight, surveys fecal nitrogen content with Kjeldahl's method.In test last day, 10 eggs of different location are chosen in every repetition
The measurement of chicken progress greenhouse gas emission.It is placed in same glass box per duplicate chicken, carbon dioxide is collected by gas outlet
(CO2), methane (CH4) gas, discharge amount is recorded with gas analyzer, measurement continues 24 hours.
(5) cause the measurement of stool odor substance:
In test the 6th week, every repetition randomly selected 3 cage chickens continuous two days and collects the sampling of chicken slurry of feces and urine daily, used sample
Bag acquisition excrement sample, weighs and records, respectively with dimethylaminobenzaldehyde colorimetric method for determining excrement sample indoles content, semi-micro-sample distillation
Measure excrement sample ammonia content, the way of distillation measures excrement sample volatile acid content.
Data statistical approach:
Test data carries out variance analysis using SPSS software, and carries out Multiple range test, and P < 0.05 is significance of difference standard.
Test result:
The influence of 1 performance in layers of table
Note: subscript difference represents significant difference, and no subscript is represented without significant difference, similarly hereinafter.
1 data of table show that test group and control group are in the production performances index such as laying rate, egg size, feed intake and feedstuff-egg ratio
Without significant difference, illustrate that laying period premix is capable of providing enough nutrition in laying cycle of laying hens, and then ensure that laying hen is normally laid eggs
Performance is unaffected.
Influence of the table 2 laying period premix to Egg Quality
2 data of table show that test group and control group are in eggshell strength, shell thickness, egg shape index, yolk color, hangh unit
With the index of the reflection egg Egg Quality such as yolk ratio without significant difference, illustrate that laying period premix is ensuring that laying hen normally lays eggs
In the case that performance is unaffected, there is no the Egg Qualities for reducing egg.
Influence of the table 3 laying period premix to Plasma of Laying Hens uric acid and blood ammonia
3 data of table show that the Blood Ammonia Concentration of test group and control group has the tendency that reducing blood ammonia without significant difference;Test group
Significantly reduce Plasma Uric Acid content.Uric acid is the metabolite of protein and nucleic acid, and Plasma Uric Acid and blood ammonia are to measure poultry
The important indicator of rate of ultilization of amino acid.High concentration uric acid can be deposited on laying hen joint and kidney, cause laying hen ventilation and kidney row
Let out burden.This test illustrates that laying period premix can effectively improve the utilization rate of feed amino acid, reduces Plasma Uric Acid content,
The waste of protein feed is reduced, and plays the role of liver protection shield kidney simultaneously.
Influence of the table 4 laying period premix to laying hen fecal nitrogen, greenhouse gases and stink substances
4 data of table show that test group and control group excrement volatile acid content are without limitation difference, carbon dioxide (CO2) and methane gas
Body (CH4) discharge difference it is not significant, but test group has the tendency that reducing carbon dioxide and methane emission (P=0.0528), examination
The nitrogen content and ammonia content, reduction stool odor substance indoles content of excrement dry matter can be significantly reduced by testing group.
Excrement ammonia, nitrogen content and stink substances seriously pollute environment, largely constrain holding for breeding layer chicken industry
Supervention exhibition, and the amino acid degradations such as the ammonia and nitrogenouz wastes of excessive amino acid degradation and tryptophan lead to the stinks object such as indoles
The generation of matter not only aggravates the metabolic burden of the organs such as liver, also has seriously polluted the environment.This is experiments have shown that laying period premix
The generation that can be effectively reduced excrement ammonia nitrogen discharge and stink substances improves henhouse ring while alleviating internal organ metabolic burden
Environmental pollution caused by excrement is reduced in border.
In conclusion the present invention provides one kind for preparing laying period premix, the present invention is according to Ideal Amino Acid Pattern
With the amino acid compositing characteristic of corn-soybean meal diet, designed by adding single amino acid in premix.Animal experiment
Show the laying period premix being formulated using premix of the invention, Layer Production Performance and Egg Quality are not generated
Negative effect, and feed amino acid utilization rate is improved, the discharge of excrement ammonia nitrogen and stink substances is reduced, it is negative to mitigate the metabolism of liver kidney
Kidney is protected in load, liver protection, improves environment, significantly reduces feed cost, improves the overall economic efficiency of breeding layer chicken.
Claims (5)
1. a kind of laying cycle of laying hens premix, which is characterized in that its main component are as follows: vitamin, microelement, amino acid, plant
Object lactobacillus.
2. laying cycle of laying hens premix according to claim 1, which is characterized in that the vitamin main component are as follows: dimension
Raw element A, vitamine D3, vitamin E, Vitamin K3, vitamin B1, vitamin B2, vitamin B6, vitamin B12, biotin,
Two or more any compositions of niacin, pantothenic acid, folic acid.
3. laying cycle of laying hens premix according to claim 1, which is characterized in that the microelement main component are as follows:
Any two kinds or more of cupric sulfate pentahydrate, ferrous sulfate, white vitriol, manganese sulfate monohydrate, Potassiumiodate, sodium selenite, zeolite powder
Kind composition.
4. laying cycle of laying hens premix according to claim 1, which is characterized in that the amino acid main component are as follows: rely
Two or more any compositions of propylhomoserin, methionine, threonine, tryptophan.
5. a kind of application method of laying cycle of laying hens premix, which is characterized in that by laying cycle of laying hens premix and complete feed
It is used in mixed way according to mass ratio 5:95.
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Cited By (1)
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CN114058538A (en) * | 2021-11-03 | 2022-02-18 | 吉林省凯博生物技术有限公司 | Lactobacillus plantarum LPJZ-658 and application thereof in improving laying performance and egg quality of laying hens |
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CN106615976A (en) * | 2017-01-09 | 2017-05-10 | 北京大伟嘉生物技术股份有限公司 | Compound premix for laying hens at laying stage used for formulating low-protein-content diet and application thereof |
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CN106615976A (en) * | 2017-01-09 | 2017-05-10 | 北京大伟嘉生物技术股份有限公司 | Compound premix for laying hens at laying stage used for formulating low-protein-content diet and application thereof |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114058538A (en) * | 2021-11-03 | 2022-02-18 | 吉林省凯博生物技术有限公司 | Lactobacillus plantarum LPJZ-658 and application thereof in improving laying performance and egg quality of laying hens |
CN114058538B (en) * | 2021-11-03 | 2023-05-23 | 吉林省凯博生物技术有限公司 | Lactobacillus plantarum LPJZ-658 and application thereof in improving laying hen productivity and egg quality |
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